Denaturing gel, Biology

Assignment Help:

Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel. 


Related Discussions:- Denaturing gel

Calculate the frequencies of heterozygotes, If dimples may be considered a ...

If dimples may be considered a single-gene dominant trait, and 64% of the population features dimples, calculate the frequencies of heterozygotes and both types of homozygotes, ass

Indications for coronary artery bypass surgery, Indications for Coronary Ar...

Indications for Coronary Artery Bypass Surgery :  The modalities of treatment for coronary artery disease iuc: (1) medical, (2) angioplasty and stenting, and (3) coronary arte

Define proline, Define Proline It is a prominent amino acid found in fi...

Define Proline It is a prominent amino acid found in fish and may contribute to sweetness. The sugars ribose, glucose and glucose-6-phosphate are flavour contributors, as is 5-

#virus, how do viruses reproduce

how do viruses reproduce

What is glycogen metabolism, Glycogen is a multibranched polysaccharide whi...

Glycogen is a multibranched polysaccharide which serves as a part of energy storage in fungi and animals. In humans the glycogen is recognized and stored primarily in the cells of

Induction in metamorphosis, Induction in Metamorphosis Some of the mo...

Induction in Metamorphosis Some of the morphogenetic changes throughout metamorphosis are found fo be quite independent of hormone action. For instance, generally during meta

Regulation of the citric acid cycle, Regulation of the Citric Acid Cycle ...

Regulation of the Citric Acid Cycle The citric acid cycle is regulated by certain enzymes and by the availability of ADP.

Outbreak of staphylococcal food poisoning, Q. Outbreak of Staphylococcal f...

Q. Outbreak of Staphylococcal food  poisoning? Conditions necessary for outbreak of Staphylococcal food poisoning: • Presence of viable staphylococcal bacteria in the food

What do protozoans eat, What do protozoans "eat"? Do they move in search fo...

What do protozoans "eat"? Do they move in search for food? Protozoans are heterotroph beings, i.e., they do not make their own food and therefore they require to search for it

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd