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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Geometric isomers: Select one: a. Have the same molecular weight b. Have the same connectivity c. Have a different configuration about a double bond d. All of the above
how many kingdom and phylum are there?
Agriculture, industry, and growing populations of cities all compete, through political influence, for water. If you were in charge of water resources in an arid region, what would
What key idea, contained in Malthus's essay on populations, helped Darwin formulate his theory of natural selection?
How is a disease-resistant plant selected for successful breeding? Why is the process of fertilisation in a flowering plant referred to as double fertilisation? Explain. Nam
phyla under protozoans
In During translation, the series of an mRNA molecule is examined from its 5' end by ribosomes that then synthesize an appropriate polypeptide. Together in prokaryotes and in eukar
Dystocia Dystocia or difficult calving is a condition where help is required as providing traction, repositioning of fetus, foetotomy or caesarotomy. Generally, ease of calvin
What is aneuploidy? What are the conditions caused by the aneuploidies? The Aneuploidy is an abnormal number of chromosomes in the cells of an individual. The major aneuploi
Pupillary reflexes There are two types of reflexes which control the pupillary reaction-the light reflex and near reflex. When light is shown on one eye, there is a constrictio
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