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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Q. Do protozoans have a cellular nucleus? All protozoans, as eukaryotes have nucleus, some species like the paramecium have two nuclei the micronucleus and the macronucleus.
Maximum and minimum range of an animal with the factors of Ph Salinity Pressure Relative human
Q. Analyze the Regional Wall Motions? Ans. Various attempts have been made in the past to try and quantify the ischemic myocardium, however till date the best and most wid
Q. Modern infection control recommendations? Concern about transmission of disease has been expressed for thousand of years. Written guidelines for "disease control" are found
extensive note on the excretory system of amphibian
How can a great biological diversity protect an ecosystem from environmental damage? Why are less biodiverse ecosystems at risk of suffering deep biological harm if submitted to ev
State one benefit of including vegetable fibre (roughage) in the diet Vegetable fibre retains water (keeping the faeces soft and bulky), stops constipation, decreases the
Conformational Protection - Azotobacter In conformational protection a Fe-S redox protein provides protection to the enzyme. The protein gets oxidised in the presence of oxyge
How is light from the sun transformed into chemical energy to be used by the living beings on earth? Light from the sun is transmitted into chemical energy contained in organic
What are the hormones that promote the release of the female gamete from the follicle and at which day of the menstrual cycle does this phenomenon happen? What is this event called
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