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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
1 ." Most of the genome does not code for protein, therefore 99% of the human genome is junk that serves no purpose". With specific reference to examples long and short non-coding
Various acute complications of diabetes mellitus are described in this unit. Acute complications develop within a short period of time. These are due to improper treatment of diabe
A woman who is heterozygous for a particular X-linked recessive trait marries a phenotypically normal man. What percentage of their sons will show the recessive phenotype?
What important changes will you find in the tissue of the fat person from those of a lean and thin person
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Protozoa classification
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