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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
It is that energy which lies, embedded within earth's crust. In some places heated water comes out through the surface of earth as hot spring or steams. This hot geothermal water o
57. While planning for an artificial hybridisation programme involving dioecious plants, which of the following steps will be irrelevant A usting of pollen on stigma B Collection
Q. What are the main interspecific ecological interactions? The major harmonious interspecific ecological interactions are: protocooperation, commensalism and mutualism. The ma
SERENDIPIDIT Y - Sometimes discoveries are made unexpectedly matter of chance. This is serendipidity. Examples - 1. Structural formula of benzene - It was dreamt by
Equine viral arteritis Equine viral arteritis (EVA) is a highly contagious disease of equines and is caused by equine arteritis virus (EAV), a RNA virus belonging to the genus
Explain the molecular difference between fats and oils. How do the differences affect their physical properties?
Define Buffers and Buffer Solutions? Solutions containing both weak acid and their salts or solutions containing weak hydroxides and their salts are referred to as buffer solut
What are the main interspecific ecological interactions? The major harmonious interspecific ecological interactions are: protocooperation, mutualism and commensalism. The major
Q. What is Rounded ST Depression? A rounded ST-segment depression pattern in the CM5 lead, as well as in the other leads, is common and is often associated with ischaemia. This
Heterochromatin: Compact, gene-poor areas of a genome, which are enriched in the simple sequence repeats. As it may be impossible to clone, heterochromatin is generally ignored wh
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