Denaturing gel, Biology

Assignment Help:

Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel. 


Related Discussions:- Denaturing gel

Briefly explain about archenteron and blastopore, Q. What are the archenter...

Q. What are the archenteron and the blastopore? What is the stage of the embryonic development in which these structures are formed? What are the destinations of the archenteron an

Oxidative decarboxylation of pyruvate, Oxidative decarboxylation of pyruvat...

Oxidative decarboxylation of pyruvate Oxidative decarboxylation ofpyruvate : This is an important step in tissues with high oxidative capacity such as cardiac muscle, whereby

How are the change in the ageing lens occurred in metabolism, How are the c...

How are the change in the ageing lens occurred in the metabolism? Changes in the Ageing Lens 1) Physical changes: Lens weight increases, light transmission decreases, li

Pathology of aortic regurgitation, Q. Pathology of aortic regurgitation? ...

Q. Pathology of aortic regurgitation? In Post Inflammatory situations scarring and retraction of valve cusps lead to aortic regurgitation. Varying degrees of commissural fusion

Explain benefits of protein, Explain benefits of Protein Protein: Once ...

Explain benefits of Protein Protein: Once the energy requirements have  been estimated, protein requirements can be addressed. The aim is to achieve nitrogen balance. There are

Explain the term phytates, Explain the term Phytates? We are familiar w...

Explain the term Phytates? We are familiar with phytates as an inhibitor of mineral absorption (calcium, iron etc.) especially in the vegetarian diets that are cereal-based. Th

Explain the chemotherapeautic rinses, Explain the Chemotherapeautic rinses ...

Explain the Chemotherapeautic rinses Chemotherapeautic rinses:  The use of  0.12  per cent chlorhexidine has been proven to be of therapeutic significance in maintaining period

What is an open circulatory system, What is an open circulatory system? ...

What is an open circulatory system? Open circulatory system is the one in which blood does not circulate only in blood vessels but it also falls in cavities that irrigate tissu

How many are the pulmonary veins, Q. What and how many are the pulmonary ve...

Q. What and how many are the pulmonary veins? The pulmonary veins are part of the pulmonary circulation they are vessels that carry oxygen-rich (arterial) blood from the lungs

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd