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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
On a given microscope, the numerical aperture of the objective is 1.5. The microscope is provided with a filter that selects a wavelength of 500 nm. What is the limit of resolution
VERTEBR A T E HEART Heart = Cardia. Most important organ of body. Main function is to pump the blood. Study of heart is cardiology. In heart auricles and ventricles pr
a) Which region of the retina gives the most accurate interpretation of the image? b) What type of light-sensitive cell is present in this region? (a) The fove
Explain about the Glycemia or Insulinemia? Preliminary research evidence indicates that there may be beneficial effects in terms of decreased fasting blood glucose/hepatic gluc
what is phylum protozoa
Ac ute heart failure The acute heart failure is characterized by sudden loss of consciousness and falling with or without convulsions. The mucous membranes become pale followe
Q. What are the segments that form the body of the tapeworm called? What is their function? The body of the tapeworm is made of segments called as proglottids. The proglottids
A simple osmometer Remove the bottom from a small glass bottle about 2.5 cm in diameter. Fit a one-hole stopper tightly into the bottom and put a 50 cm length of glass tubing o
BLOO D CORPUSCLES (= BLOOD CELLS) - Blood corpuscles are of the following three types: Erythrocytes, Leucocytes and Thrombocytes. (A ) Erythrocytes (Red Blood Corpusc
Some plants and animals originate food contain toxic substances. Some pulses and legumes contain a number of toxic substances such as protease inhibitors, lathyrogens, and flavism
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