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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
What are some examples of phenotypical characteristics that present two or more varieties and of phenotypical features that do not vary? In relation to the genes correspondent to t
Define Functions of Copper? Copper serves as a co-factor, as well as, an allosteric component of enzymes. In many enzymes copper functions as an intermediate in electrno transf
description of first five phylum
Third Week - Embryonic Development Throughout the third week of development the ICM separates from the uophoblast and forms the flattened embryonic disc, which at first consi
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give an example invertebrate phylum/phyla for protoplasmic grade organization
Define cause of vitamin a deficiency - Poverty and ignorance? Low purchasing power of the communities and their consequent inability to meet the nutrient requirements and tradi
How does temperature affect the action of enzymes upon their substrates? There are explained temperature ranges under which enzymes operate and there is a specific temperature
What is the difference between alpha glucose and beta glucose? Why are they different?
disadv of protozoa
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