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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Define Carbohydrate Metabolism of manganese? Mn is required for carbohydrate metabolism. Enzymes pyruvate carboxylase and phosphoenol pyruvate carboxy kinase involved in glucon
What is Asexual Reproduction? Asexual reproduction occurs in prokaryotes through the process of binary fission. Primitive eukaryotes and plants also reproduce by various t
why are life forms significant?
In the human body, the potassium ion can pass easily through cell membranes, yet the potassium ion concentration is higher inside many cells than it is outside these cells. Could t
Enumerate the Sizes and shapes of eukaryotic and prokaryotic cells It is thus possible, due to these similarities, to generalise about the structures and functions of cells. In
Explain sol -gel transformation During sol -gel transformation, a three dimensional network is formed by the interlocking of dispersed particles. The liquid phase is entrapped
(a) What are the two main pollutants that contribute to acid rain? (b) Where do these pollutants come from? (c) What direct or indirect effect is 'acid rain
Define Proteins as biological buffers? Proteins have the ability to accept or donate hydrogen ions and by doing so they serve as biological buffers. In blood, there are three i
Q. What are the respective functions of the micronucleus and of the macronucleus in the paramecium? The macronucleus is properly the cell nucleus it has RNA and DNA and acts as
Blotting is the technique for detecting one RNA within the mixture of RNAs (a Northern blot) or one type of the DNA within of a mixture of DNAs (a Southern blot). A blot can prove
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