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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Determine the Functions of Organ during pregnancy? Dramatic changes occur in renal functions to eliminate the nitrogenous and other waste products of maternal and foetal metabo
Functions of Endosperm The tissue of young endosperm is rich in food materials and various growth hormones. It regulates the precise mode of embryo development and nourishes
Of which type of defense cell do viral infections stimulate the multiplication? The major leukocytes that generally multiply and participate in the defense against viral infect
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Define Nutrition, Diet and Cancer? In this, we will discuss the common forms of cancer, how they develop, relevant etiological factors, pathological/metabolic changes and other
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Of which type of tissue is the heart made? How is this tissue oxygenated and nutrified? The heart is made of striated cardiac muscle tissue. The heart muscle is known as the my
Question 1 Write a short note on the following- Transfection Synthetic seeds Transgenic crops Agrometerology Question 2 Describe any 5 screening techniques use
ECOSYSTEM Plants, animals and human beings live in association with a wide variety of other plants and animals. These communities of organisms are not mere ad boc collections of
Match each of the methods or vectors below to an appropriate form of gene therapy, assigning two methods or vectors to each form. Use each method or vector only once. Methods/vec
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