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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Define the Recommended Dietary Allowances of Vitamin K? Recommended dietary intakes have not been suggested for different age groups or gentler. The safe levels of intake have
Define about the Paracellular Route - Calcium? It involves passive Ca transport through the tight junctions between mucosal cells. The salient features include: It is in
Explain Enzymes Enzymes are the proteins that act as catalysts, speeding the rate at which biochemical reactions proceed but not altering the direction or nature of th
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What are abiotic factors? Abiotic factors are the nonliving elements that constitute a given environment, as light, temperature, minerals, gases, water, atmospheric pressure, e
Alcohol Related Emergencies: Alcohol is one of the most serious health problems. Alcoholism is defined as a primary or chronic disease with genetic, psychological and environ
Describe the terms diffusion, osmosis and filtration. Explain why each process is important for the human body.
in what aspect are the cnidarians similar to the protozoans?
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GER M PLASM THEORY - It was proposed by Weismann. According to it, two types of cells are formed during embryonic development viz - Germ cell & Somatic cell. The germ ce
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