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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Q. Monounsatuated fats required for dyslipidemia? Monounsatuated fats (MUFA): These are liquid at room temperature, the highest food source being olive oil, canola oil, rapesee
Viscosity The viscosity at temperatures above its gelation point is relatively constant at pH values of 4.5 to 9.0 and is not greatly affected by age or ionic strength within
Manifestations of Hypertension • Renal Failure • Left Ventricular Failure • Myocardial Infarction • Cerebral Haemorrhage
Palaeobiology : This deals with the study of fossils and remains an impressions of the past organisms found in the rock of different ages. Paleobiology is a growing or comparativel
What are compound eyes? Arthropods have compound eyes made of many visual units called ommatidia. Every ommatidium transmits visual information by the optic nerve to the brain,
what process are nematocysts formed by the cnidoblast?
Define Carbohydrates Requirements in Human Body? Carbohydrates: High carbohydrate diets are beneficial at HA. The advantage of high carbohydrate diet is that respiratory quo
Life S cience is originated from latin word scientia , which means knowledge . Biology is a branch deals with living beings. Biology word is originated from t
Explain the Periapical surgery - Endodontic Surgery a) Curretage 1 b) Root-end ressection 2 c) Root-end preparation 3 d) Root-end filling 4
Animals in the Community - Nature and Structure of Community The animals in the community are directly or indirectly dependent on the plants also time their activities in such
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