Denaturing gel, Biology

Assignment Help:

Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel. 


Related Discussions:- Denaturing gel

Why is sub-culturing essential in plant tissue culture, Why is sub-culturin...

Why is sub-culturing essential in plant tissue culture? Explain the process of heterosis. How is it dissimilar from inbreeding depression? Where and how is urea produced in

What are the cytokinins, What are the cytokinins? And where are they made? ...

What are the cytokinins? And where are they made? The Cytokinins are phytohormones active in the promotion of cellular division they slow down the aging of tissues and act toge

Determine the procedure of armamentarium, Determine the Procedure of Armame...

Determine the Procedure of Armamentarium 1. Make an impression of the mandibular ridge and pour the cast in type 3 gypsum 2. Apply separating medium and allow it to dry 3

Biotechnology in characterization of animal biodiversity, Use of biotechnol...

Use of biotechnology in characterization of animal biodiversity. Genetic uniqueness of populations is measured by the relative genetic distances of such populations from each o

Describe briefly tyndall effect, Tyndall Effect One of the best ways to...

Tyndall Effect One of the best ways to distinguish a solution from a colloidal dispersion is to use a strong beam of intense light. As the beam passes through a colloidal dispe

How different are the actions of antibodies against bacteria, How different...

How different are the actions of antibodies against bacteria and against virus? Why is the cellular immune response activated in case of chronic viral infection? The antibodies

What is revisions, Q. What is Revisions? Revision accounts for only a s...

Q. What is Revisions? Revision accounts for only a section of a genus or for the elements as restricted to a continent or smaller geographical area. Some revisions are based so

Smoke print, Write a brief theory about smock print?

Write a brief theory about smock print?

Osmotic strength of the fluids, Osmotic Strength of the Fluids in Immediate...

Osmotic Strength of the Fluids in Immediate Surrounding The availability of soluble mineral salts varies widely from habitat to habitat. In fact, a big chunk of land in our

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd