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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
SYNAPSE - The point where 2 neurons combine is synapse. It is of 2 types - (i) Axosomic (between axon & cyton) (ii) Axodendric (between axon & dendron) Axon is term
A brief history of heart surgery : The dawn of Paediatric cardiac surgery was on August 26, 1938 when Robert, Gross, at the boston Children's Hospital, ligated ductus arteriosus
Define Inadequate Dietary Intake - cause of anaemia is dietary? The commonest cause of anaemia is dietary inadequacy of iron. The dietary intakes arc usually half of the recomm
Acute Myocardial Infarction with Haemodynamic Deterioration : These patients may require urgent insertion of intra aortic balloon pump (IABP) followed by PTCA or surgery. 50 per
Why can it be said that each glucose molecule runs the Krebs cycle twice? Every glucose molecule "cycles" the Krebs cycle twice because after glycolysis each used glucose has g
Define Non-Dietary Treatment of Burns? While good nutritional care should be provided to the patient as soon as feasible it is equally imperative and at times critical to provi
Echocardiography is a very important tool in diagnosis of cardiovascular diseases and is used virtually in all categories of cardiovascular diseases. There are certain areas where
Arteries - Circulation The arteries deliver blood from the heart. Figure shows the structure of arteries and the different layers of the vessel wall. The thick walls of these
A new drug named ANTAG-CaSR has been developed that is an antagonist at calcium-binding sites of CaSRs (Calcium-Sensing Receptors) in the plasma membranes of parathyroid gland cell
If carbon 1 is the carbonyl group of a 6-carbon aldose (aldohexose), which carbon determines if the sugar is a D- or L- stereoisomer? Select one: a. 1 b. 2 c. 3 d. 4
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