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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Q. What is the difference between diastoleSystole and systole and diastole are the two stages into which the cardiac cycle is divided Diastole is the stage of the cardiac cycle
Q. In which areas of the globe is gymnosperm abundance noteworthy? These plants are the typical vegetation of cold regions like the boreal forest or taiga of the northern hemis
Explain Indications for Surgery in Aortic Aneurysm? Indications for Surgery : Thoracic aortic aneurysms are classified by the portion of aorta involved - ascending, arch,
Can someone explain how is cancer related to ras and p53 gene? I know that the ras protein regulate cell growth and its mutation will cause cancer. How is the mutation caused?
Q. Why is it not correct to assert that the DNA self- replicates? The DNA is not completely self-sufficient in its duplication process because the replication does not occur wi
What is the essential condition for a protein to be identical to another protein? For a protein to be identical to another protein it is essential for the sequence of amino aci
Palpate the radial or brachial artery pulsation while inflating the cuff to a level of 30 mm Hg above the point at which the brachial or radial artery pulsation disappears. Reinfla
Impulses reaching the AV node from the atria are delayed a little as they pass through the trunk and crura. The impulses first reach the papillary muscles and their contraction clo
Explain in detail about the optic nerve The optic nerve contains more than one million axons that initiate in the ganglion cell layer of the retina. This structure originatcs a
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