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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
What is myoglobin? What is the function of this molecule in the muscle tissue? Myoglobin is a pigment same to hemoglobin and present in muscle fibers. Myoglobin has a great af
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Medical Nutrition Therapy Medical Nutrition Therapy (MNT) is defined as the assessment of the nutritional status of a client followed by nutrition therapy ranging Pon
Q. Explain about Malnutrition Related Diabetes Mellitus? This type of diabetes has been categorized as a separate entity. 'if is 'Men seen commonly in developing countries, 1n
The water expulsion vesicle (also known as a contractile vesicle) is an important osmoregulatory organelle in freshwater protists, which are hyperosmotic to surrounding water. Wate
Q. Chordate identity card. How are they characterized according to examples of representing beings, type of symmetry, basic morphology, germ layers and coelom, digestive system, ci
DIFFERENCE S BETWEEN MAN & RABBIT - S.NO . 1. M A N Brain round RABBIT Elongated 2. Olfa
Q. What are the hydrophobic molecules (or hydrophobic molecular regions)? What are the hydrophilic molecules? How can they be characterized in relation to their polarity? Hydro
definition of larva
Results : Pericardiectomy used to have a mortality of 10-15 per cent in the earlier era. At present it is around 3 to 5 per cent and does not approach 0 per cent even though it
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