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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
What are immunoglobulins? Immunoglobulin is the exchange name given to antibody. Immunoglobulins are complex proteins having an invariable portion and a variable portion and ma
Define Nervous System and the it's related disorders? In this unit, we learnt about nervous system and the related disorders, which are termed as 'neurological disorders'. Neur
For Dalmation dog, the spotted condition is dominant to non-spotted. a) Using a Punnet square, show a cross between two heterozygous parents. b) A spotted female Dalmation dog is m
Define Affluence and abundant availability of food as factor for obesity? With increasing affluence, increase in purchasing power and abundance of food, people tend to eat more
Now try to pull the strands apart at the "origin of replication." What happens to the DNA molecule when the ends are restricted
Bacterial food poisoning Food poisoning is an acute gastro-enteritis or intestinal disorder resulting from the ingestion of foods contaminated with either living microorganism
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Etiology Fevers can be caused due to 1. Internal (endogenous) factors: This could be caused within the body. Examples are antigen-antibody reactions, malignant cancer, graf
What Physical fitness tests examine? Physical fitness tests examine the components of physical fitness such as endurance, speed, strength, flexibility and body composition. The
Explain the Techniques of Culturing? In the last practical we learnt how to prepare culture media. This practical has been introduced to make you aware of the technique of sub-
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