Denaturing gel, Biology

Assignment Help:

Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel. 


Related Discussions:- Denaturing gel

What is phylum porifera in classification of the animal king, What is Phylu...

What is Phylum Porifera in Classification of the Animal King? Sponges are the simplest form of animals. Their bodies are not organized in any specific pattern other than masses

Muscle cells, explain why it is essential that muscle cells, during anaerob...

explain why it is essential that muscle cells, during anaerobic respiration, convert pyruvic acid(pyruvate) into lactic acid, even though the conversion results in an accumulation

Social determinants of health - unemployment, Social Determinants of Health...

Social Determinants of Health - Unemployment That unemployment gives rise to social insecurity and psychological stress is well recognised. Fears and uncertainties are known t

Digestion of carbohydrates, Digestion of Carbohydrates Digestion of foo...

Digestion of Carbohydrates Digestion of food starts in the mouth  itself by the action  of  enzyme  salivary  amylase  and  the  carbohydrates present  in  the food, particular

Leucosolenia, characteristics of leucosolenia?

characteristics of leucosolenia?

How are fishes characterized, Q. Fish identity card. How are fishes charact...

Q. Fish identity card. How are fishes characterized according to examples of representing beings, skin, basic morphology, circulation, nitrogen waste, respiration, thermal control

Describe the implant in an infected socket, Placement of the Implant in an ...

Placement of the Implant in an Infected Socket or a Pathologic Lesion Dental implants may fail due to: 1) Placement of the implant fixture into an infected socket (during im

Cell coat and its functions, CELL COAT (GLYCOCALYX) Present in Proti...

CELL COAT (GLYCOCALYX) Present in Protists and animal cells. Usually made of oligosaccharide side chains of glycolipids and glycoproteins. Cell coat has very charged t

Cancer, Cancer ;  Cell  division , like  all other  biological  processes,...

Cancer ;  Cell  division , like  all other  biological  processes,  is under  genetic control. Certain  genes must regulate the processes  of cell growth  and division  in respons

Differnces, difference between protonephridia and metanephridia

difference between protonephridia and metanephridia

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd