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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Q. Explain about Peptic Ulcer? Peptic ulcers are one of the more serious forms of gastrointestinal disorders of the gastrointestinal tract particularly the stomach. The prevale
J-shape d - It shows 2 phases - (i) Lay phase (ii) Exponential phase
Kidney impairment is one of the long term complications of diabetes mellitus. Measurement and monitoring of kidney function parameters such as urea and creatinine, in addition to u
respiration
Four major groups of lipoproteins help in transport of lipids. These include: 1. Chylonzicrons-function is to carry dietary triacylglycerols 2. Very low density lipoprot
Rice milling Rice milling involves the following processing steps: rough rice (paddy rice) → hull removal → brown rice → polishing to remove the bran coats (fruits and seed
Define Physical and Physiological Changes? Every stage has its unique requirements due to different changing needs. With respect to nutrition and health, four different basic a
Central Nervous System (CNS) Covering of the brain and spinal cord is called Meanings (membranes which cover and protect brain and spinal cord are called meanings) There is flu
Define the Skeletal muscle A. Binding of calcium ion to its receptor site on the actin molecule blocks the attachment of the head of the tropomyosin molecule to its binding sit
What are the functional divisions of the nervous system? Functionally the nervous system can be separated into the somatic nervous system and visceral nervous system. The so
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