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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Describe a health issue or disease and recommend an intervention (you may utilize the health issue . Justify the intervention level (primary, secondary and tertiary) and setting ch
What are the lateral lines of fishes? The lateral lines of bony fishes are sense organs that extend along both sides of the animal body. They make contact with the environment
ASPIRIN - It is acetylsalicyclic acid (C 6 H 4 (OCOCH) 3 CH 3 ). It changes in salicyclic acid in body. It is analgesic, antipyratic (fever reducing), anti inflamma
Define Thermal Coagulation and Film Formation? 1. Thermal Coagulation and Film Formation: Concentrated soy proteins can be thermally coagulated on a flat metallic surface, such
A double-stranded piece of DNA, 100 base pairs in size, is exposed to a high pH environment. This causes all non-valent interactions to be disrupted. What will result from this tre
Explain the concpet of Dressing Dressing: Means medicine wound covering; a bandage or other sterile covering is put on a wound to protect it from infection or further damage.
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State the similarity between Shigella and Salmonella There are many points of similarity between Shigella and Salmonella. They dwell primarily in the gastrointestinal tract, wi
Over the last three decades medical imaging has developed to be an important tool for medical condition diagnosis, treatment planning and surgery. The insight provided by informati
What are plankton, benthos and nekton? Plankton, benthos and nekton are the three groups into which aquatic living beings may be divided. The plankton is formed by algae and
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