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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
explain the different types of symmetry in metazoans.
wine turns sour beause of the action of which bacteria? aerobic or anerobic?
Define Categories of Indicators of Malnutrition? The indicators may fall under the following categories: 1. Macro indicators for sector-wide monitoring and evolution, 2.
what is Algae
genral charecter and classificatin of porifera
Define Flow Phase in Physiological Response to injury? It is characterized by hyper metabolism, increased cardiac output, increased urinary nitrogen losses, altered glucose met
Explain about the Nutritional Support Management? Tube feeding is usually started. If tube feeding is not possible, parenteral nutrition through peripheral vein or through the
Q. Use of Examination Gloves? Examination Gloves - latex, vinyl, nitrile, neoprene Gloves are worn whenever contact with blood, saliva, mucous membranes or blood/saliva-cont
Osmotic Function - Essential Elements Plant cells generally contain mineral ions 10 to 1000 times higher in concentration than the surrounding soil. That is why water enters t
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