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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Q. What is signifying when it is said that a bacteria is an obligate anaerobe? Obligate anaerobes are those living beings that do not survive in the presence of oxygen. For exa
Beta-blockers have traditionally been considered contraindicated in patients with heart failure because they may block the compensatory actions of the sympathetic nervous system wi
What is Ascending Aortic Aneurysm ? Ascending Aortic Aneurysm : In aneurysms confined to ascending aorta well below the innominate artery origin, the technique used is
Define Overall positive health Effect of Carbohydrate? Non-glyceinic carbohydrates including non-starch polysaccharides are beneficial for the functions and physiology of gastr
Q Why after the passage of animals from the aquatic to the terrestrial habitat does the abandonment of the ammoniotelic excretion occur? Ammonia is a highly toxic molecule if n
Benefit of the new brain imaging technology Benefit from the new brain imaging technology, notably the CT scan, and the application of high speed computer technologies, permitt
Phosphodiesterase Inhibitors and Other Agents Amrinone and Milrinone are prototypes of type III PDE inhibitors. They decrease the breakdown of cyclic AMP in the myocardium and i
structural adaptation of mammalian alimentary canal
describe food procurement and digestion in ciliates.
Q. What is polyembryony? Polyembryony is the phenomenon in which a single embryo in its initial embryonic stage divides itself forming many new individuals of genetically ident
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