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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
A group working at the University of California, Berkeley has developed "nanothermometers," little nanoparticles that can be injected into cells to measure the temperature in vario
Which of the following serves as a sensor, or as part of a sensor, that functions in a negative feedback system? A. CaSRs (Calcium-Sensing Receptors) located in the plasma memb
Unlike DNA binding domains and dimerization domains, no general structural motifs have since been identified in the activation domains of diverse transcription factors.
Classification of plants and animals
Which of the following statements concerning enzymes is false? a. They function as catalysts b. The active site of an enzyme has a shape that is specific for its c. Many e
Question 1: (a) Describe the maturity stages in fish. (b) "k" and "r" selection strategies are commonly used to describe the response of fish to biotic and abiotic cond
SIMPL E PROTEINS The proteins are made of amino acids only. Additional chemicals are absent. These are of two types - (i) Fibrous (ii) Globular ( i )
what are the phylum of kingdom protozoan
Are hormones only proteins? Some hormones are proteins, such as insulin, glucagon and ADH, others are derived from proteins (changed amino acids), like adrenaline and noradrena
Oxidized starch Coating and sealing agent, emulsifier, dough conditioner for bread, binding agent as gum Arabic replacer, lemon curd manufacture, salad cream, mayonnaise.
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