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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Moss Stage - Xerarch The accumulation of soil, particularly in the crevices and depressions of rock favours the growth of certain xerophytic mosses, e.g., species of Polytrich
Define Nutritional Requirements and Recommended Allowances for Proteins and Amino Acids? The FAO/WHO Committee (1973) expressed the protein requirements in terms of egg or milk
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Describe Respiratory System? The respiratory system is responsible for the transport of oxygen and carbon dioxide from outside the body to and from the blood. This process is c
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