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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Hom o sapiens fossilis (CRO-MAGNO N MAN) - Fossils found from Cro-Magnon, France by Mac Gregor (1868) Replaced Neanderthal man about 35.000 years ago Sharp nose
Q. What are the main respective constituents of cell walls in bacteria, protists, fungi and plants? In bacteria the cell wall is made of peptidoglycans, amongst protists algae
How does the sexual arousal mechanism in women facilitate fecundation? During sexual arousal in women the vagina secretes substances to neutralize its acidity therefore allowin
The nutrition care process acknowledges the common dimensions of practice by the following: Defining a common language that allows nutrition practice to be more measurable,
Alkaline slant - Observation for Carbohydrate Utilization Pattern Test Alkaline slant (red) and acid butt (yellow) with or without gas production - This indicates fermentation
Functional properties of proteins These are those physico-chemical properties that enable the proteins to contribute to the desirable characteristics of the food Potential f
Q. What is the main molecule responsible for the absorption of photic energy for photosynthesis? And where is that molecule located in photosynthetic cells? The chlorophyll mol
SCLEROTI C - White of eye. Outer thick, made up of fibres connective tissue. Protective layer. Give definite shape. Its anterior 1/6 part is thin, transparent known as
During our discussion of replication origins we took a step-by-step look at the method of cloning DNA fragments into plasmids . Based on your knowledge of this discussion which
Draw decanol, plamitic acid (C16:0 fatty acid) and the resulting wax ester generated by a dehydration reaction between both molecules
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