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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Define about the Lactose? Lactose has a molecular structure consisting of galactose and glucose. It is of interest because it is associated with lactose intolerance which is th
What is Interspecific Competition in ecology explain? Populations in nature usually share their surrounding environment in time and space with populations of other species. If
Why can it be said that the enzymatic action is highly specific? The enzymatic action is highly specific because only exact substrates of one enzyme bind to the activation cent
Measurement of Nitrogenase Activity There are various methods to find out whether an organism is a N 2 -fixer or not. If an organism can grow in normal atmosphere without any
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Q. What is Interpretation of Stress Echocardiography? The standard approach to interpretation is qualitative. The attendant need for training and problems posed for reproducibi
How are the excretory systems of the three main arthropod classes constituted? In crustaceans a pair of excretory organs known as green glands exists. The green glands collect
OBSESSIVE COMPULSIVE DISORDER (OCD): OCD is another common neurotic disorder seen in the hospital. Definition Obsessive Compulsive Disorder is the disorder characte
Multiplication Phas - Stages of spermatogenesis The initial cells in the germ line arc known as primordial germ cells (PCC). The PGC, which arise at some distance from the pro
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