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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Explain the Features of Alternaria? 1. Alternaria colony is wooly and compact. Underside is very dark coloured. Colony colour is grayish green or black with gray edges rapidly
Q. What is the treatment process of mitral stenosis? Presence of symptoms or any complications is an indication for treatment. Apart from penicillin prophylaxis no other treatm
Relation between taxonomy and ecology
Each ribosome having of two subunits a small subunit and a large subunit, every of that is a multi component complex of rRNAs (ribosomal RNAs) and ribosomal proteins t
Q. What Treatment should be used for cardiac tamponade? Percutaneous Pericardiocentesis Cardiac tamponade is an acute emergency and percutaneous pericardiocentesis must b
Explain the Objectives of Taxonomy Let us summarise the objectives of taxonomy: 1. To inventory the world flora and fauna 2. To provide a method for identification and co
Thorax and Lungs: The lungs, a pair of conical-shaped organs lie in the thoracic cavity, protected by the bony thorax composed of the sternum and ribs a interiorly and r
Difference between unconditioned and conditioned reflex action- S.NO . 1. UNCONDITIONED It is inborn CONDITIONED
This is concerned more with nature's services which also make vital contributions to the welfare of society and to ecological processes without which our planet would be uninhabita
Explain the Periodontal consideration Periodontal consideration would include: Hard Calculus Build up: Any index assessing the amount of calculus build up can be used .
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