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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Calculate the molar concentration of illinic acid and of its conjugate base illinate ion (in moles/L; M) in a 0.002 M illinate buffer at pH 12.0. The pKa of illinic acid is 6.7. En
Explain carbon and oxygen aspect of using phase rule? Consider a system containing solid carbon (graphite) and a gaseous mixture of O 2 , CO, and CO 2 . There are four species
Name the corrosion resistant metals Most of the corrosion resistant metals or alloys used for implants have a layer of oxides on their surface. The thin (approximately 5 nm) la
Explain the Methodology for seliwanoff test? Take 5 ml of seliwanoff's reagent in a test tube. Add 5-6 drops of the glucose solution and heat the mixture to boiling for about 3
why is obelia considered to of special interest in Zoology as an animal showing an intermediate grade of organisation?
The second category of isolating mechanism that may permit interspecific mating but ensures reduced viability is called post-mating isolating mechanism. This category could be subd
Explain Precautions for Gram Staining of Bacterial Cultures? 1. Heat fix the smear, otherwise cells would be washed off. 2. Fresh 24 hrs old culture should be used. Gram var
Neural Basis of Responses to Sex Pheromones The ability of insects to detect and discriminate between a variety of pheromones with great sensitivity depends on the presence of
What is the karyotype found in Down syndrome? Down syndrome is the aneuploidy that is a numeric alteration of chromosomes within the cells compared to the normal number of chro
what is role of macro and micro nutrients
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