Denaturing gel, Biology

Assignment Help:

Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel. 


Related Discussions:- Denaturing gel

Define the high blood plasma levels of progesterone, Z is a healthy adult f...

Z is a healthy adult female who ovulates in each menstrual cycle; Z is not pregnant.  During Z's menstrual cycle, high blood plasma levels of progesterone   A. Occur just prior

What is oligosaccharides , Oligosaccharides are short chains of m...

Oligosaccharides are short chains of monosaccharides linked together by glycosidic bonds. In case of oligosaccharides  linked to proteins (glycoproteins)  or lipids (glycolipids) o

Systolic dysfunction onset of symptoms, Q. Systolic dysfunction onset of sy...

Q. Systolic dysfunction onset of symptoms? In asymptomatic patients with normal LV function, the rate of progression to symptoms and/or LV Systolic dysfunction is about 4 per c

Antibodies, Antibodies Antibodies are important.tools for detecting and...

Antibodies Antibodies are important.tools for detecting and localising specific molecules in the cells due to their high specificity. The first requirement for this is to produ

What is the disease caused by dietary iodine deficiency, Q. What are few ex...

Q. What are few examples of mineral salts from the diet that act as coenzymes? Magnesium, copper and zinc are examples of biological coenzymes. Q. What is the disease cause

Define categories of fatty acids, Define Categories of Fatty Acids? The...

Define Categories of Fatty Acids? The fatty acids can be discussed under following heads: Saturated and Unsaturated, Short chain, medium chain and long chain,

Tricuspid valve - heart, It is made up of three cusps, anterior, posterior ...

It is made up of three cusps, anterior, posterior and septal. The septal cusp is attached to the medial margin. The anterior cusp is attached to the superior-lateral part of the ma

Explain the control of air pollution, Explain the Control of Air Pollution ...

Explain the Control of Air Pollution a. Air belongs to everyone Need it to treat air pollution as public problem   b. Air pollution is an inevitable concomitan

Nutrion, what are the different modes of nutrition

what are the different modes of nutrition

Phimosis, Phimosis Phimosis may be congenital or acquired. The foreski...

Phimosis Phimosis may be congenital or acquired. The foreskin in young infants cannot be retracted, because at that gge it is adherent to the underlying glans. Wlen an attemp

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd