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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Telenursing Telenursing refers to the exchange of expertise in various areas like nursing diagnosis data over a distance. It is divided into the following categories: a)
Q. What is the defense mechanism that begins to work when inflammation fails to stop an infection? If the inflammatory attack is not sufficient to halt the infectious process t
Similarities between autotrophic succession and heterotrophic succession
Explain Precautions for preparation of general purpose media 1. Dissolve ingredients one by one in distilled water. 2. Check and set the medium to appropriate pH. 3. Auto
Rifaximin (Xifaxan) An anon-absorbed oral antibiotic, was about as effective as ciprofloxacin for treatment of travelers' diarrhea, mostly caused by E. coli. It is not effecti
Q. Dietary Recommendations during Diarrhoea? The diet should take into account the normal RDI and various adjustments made to the quantity and quality of the foods to be given.
AMPHE T AMINE S - Commonly called pep pills or antisleep drugs. They increase physical & mental performance. Taken by truck drivers, student & night workers to keep
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Q. What is the binding between two amino acids called? The chemical bond between two amino acids is called a peptide bond.
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