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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Explain Adverse Effects of ganciclovir Ganciclovir is teratogenic, carcinogenic and mutagenic and causes aspermatogenesis in animals. Granulocytopenia and thrombocytopenia, whi
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Define Sit and Reach Flexibility component in the humans? The subject sits on a mat with legs extended straight ahead. Legs should be at right angles to a taped the r box, with
Q. How does vitamin C act in the body? What is the harm caused by insufficiency of vitamin C? Why was this deficiency also known as "sailors' disease"? Vitamin C, or ascorbic a
Which of the following is true for a toe motor neuron that excites a toe muscle that moves the big toe in the right foot? A. The cell body of the toe motor neuron is located in
Define Water Distribution and Compartments of Body Water? Each one of us has a veritable 'sea within', held in place by multiple membranes and our protective envelope of skin.
Explain the Regulation of blood pressure? The renin-angiotensin system regulates the blood pressure. The synthesis of renin is decreased by calcitriol through its interaction
A woman who is heterozygous for a particular X-linked recessive trait marries a phenotypically normal man. What percentage of their sons will show the recessive phenotype?
compare between their phenotypes
Q. Illustrate Hypertrophic cardiomyopathy? It is a genetic disorder due to mutations in the gene that encodes for β-Cardiac myosin heavy chain (Localised to chromosome 14). It
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