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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
What is the stage of cellular respiration during which carbon dioxide is liberated? In aerobic cellular respiration the release of carbon dioxide occurs in the transformation o
Define Carbohydrates needs in Postoperative Nutritional Care? Carbohydrates ensure the use of protein for tissue synthesis and energy required for increased metabolic demands.
Define Polysaccharides (DP: > 9) - carbohydrates? Polysaccharides are high molecular weight polymers of monosaccharide units formed by glycosidic bonding. They may be long unbr
Cancer Lung Incidence of cancer lung is more in persons who smoke. Environmental and industrial pollution is also a risk factor in the increase in incidence. Clinic
Q. Differentiate between sterilization and disinfection? 1) Sterilization is defined as freeing the object or substance from all life of any kind. It is the process by which a
What process causes oxygen to pass from the alveoli into the lung capillaries? Diffusion is the process by which oxygen passes from the alveoli to the lung capillaries.
Define Risk assessment Risk assessment has been described as the process of organizing information concerning a particular risk in a systematic way so that the dec
Q. What are the main risk factors for hypertension? The major risks factors for hypertension are tobacco smoking, stress, obesity, sedentary lifestyle and alcoholism.
Illustrating how neuropsychologists evaluate young preschool children, older children, youngster adults, and elderly adults. The brain is an evolving organ and functions very
composition of aquatic animals what are their groupings
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