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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Strawberries are quick frozen at a rate of 6500 kg/h. The fruit enters the freezer at a temperature of 15°C and is frozen to a final temperature of -20°C. Calculate the rate of hea
Define Foods Effect on Drug Transport? Many drugs are transported in blood bound lo plasma proteins. Severe malnutrition or diseases affecting the synthesis of plasma proteins
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It is the process of separating colloidal and suspended impurities from water by passing it through a porous bed made of fine sand and other proper sized granular materials. Filtra
Define respiration in animals
Discuss about the function of Tear Film in short. Functions of Tear Film The normal function of the eye is dependent on sufficient supply of tear fluid- a secretion
State the Classification of nervous system Neurological disorders can be categorised according to the primary location affected, the primary type of dysfunction involved, or th
Q. Clinical Features of tricuspid regurgitation? Patients with severe primary tricuspid regurgitation will have symptoms of fatigue, dyspnoea and effort intolerance, abdominal
Use of Chlamydia in Pregnancy Doxycycline, other tetracyclines and the flour quinolones generally should not be used during pregnancy. Azithromycin appears to be safe in pregn
Tricuspid Excision : For tricuspid valve endocarditis with vegetations in drug users, valve excision is done by removing three cusps and chordae and papillary muscles. Later
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