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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Disease occurrence due to climate change The movement of animal diseases across physical and political boundaries threatens food security and creates a global public co
Q. What are the uses of National parks ? National Park is an area dedicated to conserve environment, natural and historical objects and to conserve the wild life therein. Natio
What are the kinds of plant geotropisms? Why do the root and the stem present opposite geotropisms? The kinds of geotropisms are the positive geotropism, that in which the plan
Is dwarf pampas grass invasive? No. the root system is shallow and extends only as far as the plant is large. Though if the irrigation system is installed poorly and the pipes
What is Life threatening haemorrhage Life threatening haemorrhage has been reported when a drill perforates the sublingual region of the mandible and injures the sublingual arte
Explain the Stool weight and laxation? The amount of stool excreted varies markedly from individual to individual and in an individual over a period of time. Faeces are complex
Define Paediatric Problems and Nutritional Management? The process of accepting and digesting food in adequate amounts to meet nutrition needs is termed as feeding. Certain gro
In yeast, a new mutation called suc was recently discovered. The suc mutant cannot use sucrose as a source of carbon, and will not grow if the culture medium contains only sucrose
What are the principal sources of of excessive nitrate and phosphatebin rivers and lakes?
Q. Show the types of oesophagitis conditions? The two types of oesophagitis conditions: 1. Acute Oesophagitis - It is characterized by substantial pain on swallowing. It is
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