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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Restricted Food Items Foods to be avoided by diabetic patient are carbohydrate and fatty food. Carbohydrates are classified into sugars and starches. As you have studied in und
Gaseous fuels are increasingly used in industrial as well as domestic uses. Gaseous fuels have very high calorific value and comparatively higher thermal efficiency than solid and
L iq uid manure handling This involves use of lot of water to flush the manure in to collection tank. Storage capacity depends on type of farm, irrigation facility, storage t
what is the genetic code of life
Define Plate Counts and Colony Forming Unit? Plate counts is the most frequently used method to measure microbial numbers. The technique, however, can be used only for those mi
Q. Besides the skin what are the other coverings of the body? Besides the skin there are other covering tissues made of epithelium over other tissue layers. They are the tissue
Explain the Pipettes - Food Microbiology? Sterile glass pipettes or disposable pipettes can be used for transferring the known volume of liquid or culture aseptically. Steriliz
1. By direct measurement of the activity of the thyroid gland in several patients who had received 131 I for diagnostic purposes, it was observed that the biological elimination h
Explain the Penicillium - Fungi? Classification Kingdom - Mycetae Division - Amastigomycota Class - Ascomycetes Subclass - Plectomycetidae Order - Eurotiales
AGA R - AGAR It is cell wall mucopolysaccharide of some red algae (e.g. Gelidium, Gelidiella, Gracillaria). Agar is used as culture medium, thickener, stabilizer and as lax
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