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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
We have no antibodies against any blood group when we are born still we say that some blood groups are incompatible for us.why?
Some species of plant are strongly adapted to pollination by certain insects. Characteristics which are regarded as adaptations to pollination by bees are: ( a) white o
Where do the two neuronal pathways diverge For both the neuronal pathways i.e. light reflex and near reflex the efferent fibres go to are 3rd cranial nerve and then to sphinct
What is telokinesis and how is it different in animal then plant cells?
In the scientific competition against fixism what are the main arguments that favor evolutionism? The major arguments in favor of evolutionism are paleontological, from the stu
Explain brefiely the chemical equations? Chemical Equations : A chemical equation is a shorthand way of describing a chemical reaction. For example, in the formation of wate
What is Form Sense Form Sense is the faculty which enables us to perceive the shape of objects in the outer world. Here the cones play predominant part, and the form
justify the claim that paramecium is the highly evolved protozoan basing on the morphological and physiological features .
Q. Explain Spoilage by yeasts? Yeasts dominate in the spoilage of fruit products which contain high acid content due to their ability to tolerate high acid environment. Yeast
How does brain recognize difference between high and low c and soft and loud sounds?
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