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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Single Cell Proteins (SCP) The term SCP was coined by Prof. Caroll Wilson (MIT) in 1966. It means the proteins obtained from microbial sources, i.e. algae, fungi, bacteria,
Cell culture products: Animal cell cultures are used to generate virus vaccines, as well as different kind of useful biochemical which are mainly high molecular weight prote
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Explain Personal Risk Factors in CAD? The personal risk factors are the factors that are specific and characteristic for a particular individual and are non-modifiable in natur
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