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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Discuss about the Common migraine This is the most frequent type, occurring in more than 80% of migraine sufferers. There is no clear aura as there is in classic migraine, but
Define Nutritional Requirements of Fats and Oils during Pregnancy and Lactation? ICMR (1990) recommends that linoleic acid requirements should be raised to 4.5 en% during pregn
(a) What effect does alcohol have on reaction time? (b) What other short-term effects does alcohol have? (c) What long-term effects can result from an exce
What phrase best describes oxidation- reduction reaction? A. phosphoryrylation energezing endergounic reactions b. transferring of electrons from one molecule to another c. shar
Apex locator in retreatment cases: - Electronic apex locators may give misread for working length when gutta-percha is initially being removed. - Due to that file beging
What are the chemical elements that form most of living biological matter? The elements are:- a) oxygen (O), b) carbon (C), c) hydrogen (H) and d) Nitrogen (N).
Types of Axons Axons are of two types Myelinated and Non-myelinated In the case of myelinated axons the neurilemma is ensheathed by another cover
Precautions It is a common observation that BP recordings are variable when taken by different individuals. To decrease this variability it is important that certain precautio
Right coronary artery arises from the anterior aortic sinus. It runs down along the anterior part of the coronary sulcus, reaches the lower border of heart turns round the lower bo
Suppose many of the atoms of nitrogen in a double-stranded molecule of DNA, of isotope N-14, were replaced with the isotope N-15. What afffect would this have? a) The nitrogeneo
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