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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Explain the diagnostic set-up surgical stent The patient should understand the procedure and be warned of any complications. They should have agreed with the treatment plan, t
Diagnostic Models Study models made from impressions of the arches are invaluable for diagnosis and treatment planning. The model must be mounted on an adjustable or semi adjus
Explain Post Myocardial Infarction Surgery Indications ? Indications for Surgery : Presence of ventricular septal rupture is an indication for surgery. Timing of surgery is
Distinguish among the terms 'pesticide', 'insecticide' and 'herbicide. A pesticide is a compound which destroys or controls any organism which is considered to be harmful
ISOZYMES Sometimes an enzyme present in the same organism is found to have different molecular forms but catalyzing the same reaction. These are called isozymes. Th
What is an argument that shows that the emergence of photosynthetic beings was crucial for life to reach the marine surface and later the dry land? The Ultraviolet radiation fr
Q. What do you mean by Ulcerative Colitis? Let us understand clearly about ulcerative colitis by reading the following case. Varun, a 48-year-old male, had a very successful
Why is drosophila a convenient animal for the study of linked genes? The fruit fly drosophila is appropriate for the study of Genetics because it presents many distinct traits
Protection of protein meals or bypass protein The protein of oil cakes, having higher protein degradability, such as groundnut cake, mustard cake are utilized in the rumen les
What are the major terrestrial biomes? The main terrestrial biomes are: tundra, taigas (or boreal forest), tropical forests, temperate forests, grasslands and deserts.
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