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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Define the Fats requirement to avoid underweight problem? We know that fats are concentrated source of energy (1g = 9 Kcals). Fats are capable of increasing the energy val
cutaneous respiration in musquito
WHAT ARE THE DIFFERENT CULTURE MEDIAS USED FOR ISOLATION OF ACTINOMYCETES?
Discuss the example of Comprehensive neuropsychology Example of these include deficits of visuo-spatial perception hampering performance on construction tasks, poor comprehe
pour plate method
Explain Fungi - Nutritional Types of Microorganisms? Fungi are filamentous, eukaryotic microorganisms, ubiquitous in nature. These grow best in dark and moist habitats. Their h
ELECTRON TRANSPORT CHAIN All processes require energy. In living cells, we constantly use energy for a number of biochemical reactions e.g. muscular movements, synthesis of ne
I have a six page assignment, which involves barely any writing, just labeling some plant structures etc. Can someone do this?
Q. What are some examples of biological activities in which osmosis plays an significant role? Hemolysis destruction of red blood cells by entrance of water, the hydric regulat
Q. What do you mean by Recovery Period? At the instant exercise is discontinued, the ECG recorded is turned on and left running for a few seconds while the blood pressure is re
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