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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
There are two types of Doppler examination: 1) Pulse wave Doppler 2) Continuous wave Doppler Both these modalities are necessary part of Doppler echocardiography and pro
what is the process of nitrogen fixation in soil by bacteria
Q. How is the mesoderm (third germ layer) of triploblastic animals formed? The mesoderm shows from differentiation of endodermal cells that cover the dorsal region of the arche
Interferons are small glycoproteins formed by virus-infected cells that inhibit viral infection. They are heterogeneous. Gamma interferons induce MHC class II antigens in macrophag
Q. What is the formula of the net primary production (NPP)? How does NPP relate to the energy pyramids? The Net primary production is the gross primary productivity less the or
what is a vaso-constriction? what are the effects of vaso-constriction in the skin?
How many cells are in the average human brain? Roughly, 6 trillion are in the average human brain.
Carbohydrates are the next important nutrients as they have to perform a variety of functions such as: 1) Provide major source of energy 2) Breakdown of fatty acids and preve
Rectum check for anal patency and passage of meconium and observe whether meconium is passed or not. Confirm its patency then pass a catheter or gloved finger check if
Differance between Descending Thoracic or Abdominal Aneurysm ? Descending Thoracic Aortic and Thoraco-Abdominal Aneurysm : The approach is through n left postero-lat
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