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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
The vascular tone increases as the arterial size decreases. The tone at any time reflects the effects of excitory and inhibitory pathways. Neurotransmitters, physical forces like s
Shows evidence of the ability to see the interaction between environment / ecology and genetics / evolution Some candidates may recognise that evolution can result in less no
SKIN - Largest organ of body. It is covered by hairs or pelage i.e. pillifera. Study of skin is dermatology. Outermost covering of body. Skin is elastic and
Define Secondary prevention- preventive strategies for food allergy? Secondary Prevention: Attempts to inhibit expression of the disease despite sensitization. These are used f
Pyramid of Numbers - Ecological Pyramids A graphic representation of the total number of individuals of different species belonging to each trophic level in an ecosystem is kn
Autogamy - Patterns of Sexual Reproduction It is known to take place in many ciliate protozoans including Paramecium. Autogamy involves same nuclear behavior like in conjugati
Differentiate between spread plating and streaking?
Hydrogen bonds are important for all of the following except:: a) Allowing carbohydrates to dissolve in water b) Stabilizing the three-dimensional shape of proteins. c) Ma
What is Active site Active site : The site on the surface of an enzyme to which substrate or substrates bind.
Sensory Receptors Sensory information received by these receptors is transmitted through sensory fibres of peripheral nerves to sensory areas at different levels of spinal cord
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