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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Explain citric acid cycle In the citric acid cycle, the oxaloacetate is first condensed with acetyl CoA, and then regenerated as the cycle is comp!eted. But these react
Eucaryotic Cell Organelles A eucaryotic cell has excessive foldings of intrace!:u:;ir cembrane as compared to procaryotic cell. The eucaryotic cell has a number of'organelles s
Q What is the usefulness of fungi for some industries? Fungi are industrially used in the production of fermented beverage, cheese, bread, and so on. A few fungi are very impor
Classify phylum mollusca?
Question 1 Write short note on inflammatory response 2 Give an account of attenuated vaccines 3 What is innate immunity? Explain its characteristic features 4 What adjuvants? E
This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or oth
Role of Pancreas in Digestion Pancreatic juices secreted from the pancreas aid in digestion of the food. About 600-800 ml of fluid i.e. the pancreatic juice is
Explain about the Calcium and Hypertension? Chronic inadequate intake of calcium may play some role in etiologies of hypertension. Calcium deficiency has been linked to hyperte
Primitive Arthropods The primitive arthropods, Onychophora (for example Peripatus) have a series of paired legs which are not jointed but have a ringed appearance because of t
Q. Why are most ammoniotelic beings aquatic animals? Aquatic animals like crustaceans, amphibian larvae and bony fishes generally are ammoniotelic since ammonia diffuses more e
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