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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Q. Show Classification of englearn dPrantls system? The outline and basis of classification of englearn d Prantl's system are given below: 1) Plant Kingdom has been divided,
general characteristics of sponges
Similarities in cloning and selective breeding cloning (not transgenesis) and selective breeding both transfer whole genome both selective breeding and cloning have t
What is the approximate pH of the salivary secretion? Is it an acid or basic fluid? What are the main functions of saliva? The saliva pH is approximately 6.8. It is therefore a
Q. What do you mean by Binomial nomenclature? Binomial nomenclature is the naming of the individual entity and the group so produced through classification, according to the in
What are compound eyes? Arthropods have compound eyes made of many visual units called ommatidia. Every ommatidium transmits visual information by the optic nerve to the brain,
Define Food quality factors and their measurement? Appearance; textural, flavour, nutritional, sanitary and keeping factors; quality standards, objective and organoleptic evalu
Q. Discuss in brief distant and contact osteogenesis? Three terms are used to describe individual aspects of bone formation process that occur at implants. They are Osteocondu
Hydroxyapatite coating Hydroxyapatite coating has been recommended for clinical use. These implants are superior with respect to the degree and rate of fixation in the bone as
#question.what are the reason that arthropodans are abundant in nature.
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