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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Respiratory System in Living Organisms Respiration is a necessary physiological process in all living organisms by which they obtain energy for carrying out all the metabolic
Explain theTechnique of total anomalous Surgery ? Corrective surgery is undertaken as an emergency procedure after diagnosis has been confirmed by two-dimensional echocardiogra
Q. What are the parathyroids? Where are they located and what are the hormones secreted by these glands? The parathyroids are four small glands embedded two in each posterior f
Sulphur is one of important of proteins vitamins and hormones. It plays a very crucial role in health of both plants and animals. Most of earth sulphur is tied up in rocks and salt
What are the main degenerative diseases of the heart? The major degenerative diseases of the heart are heart failure, arrhythmias, valvular heart diseases, coronary insufficie
What are the differences between blood retinal barrier and blood aqueous barrier? Blood Retinal Barrier: Blood retinal barrier is present in retina. In retina there are t
Q. How is the finding of ribosomes inside mitochondria and chloroplasts explained? It is a strong hypothesis that chloroplasts and mitochondria were prokaryotes that associated
Q. Can Obesity causes the cardiovascular disease? As you know obesity or excessive weight is the primary cause of cardiovascular disease. It is an independent risk factor for h
Penicillin, discovered by the Scottish doctor Alexander Fleming, in 1928, is a drug that inhibits enzymes essential for the synthesis of peptidoglycans, a constituent of the bacter
Q. Can High blood pressure cause coronary sclerosis? High blood pressure causes coronary sclerosis (hardening in early stages, fatty lesions in the inner surface of [he artery
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