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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Define Future Projections in the Field of Public Nutrition? We discussed earlier that the field of public nutrition has existed for a long time, although not by this name. A he
Q. What is H shaped incision and what is it's purpose? This type of incision is especially useful for: The anterior maxilla or mandible. Accurately identified implant
What is the best microscope to get a detailed view of the parts inside of a preserved plant cell? Transmission electron microscope Scanning electron microscope Compound li
The Starch Granule - Structure Starch is a polysaccharide, a chain of many glucose molecules. There are two types of glucose chains in starch. One is a simple chain called am
PHOSPHOLIPIDS Most abundant lipid present in cell membrane, also called membrane lipid. It is made up of lipid & phosphoric acid. The basic phospholipid is phosphat
Q. What are the dietary guidelines for gastritis? Energy: Give adequate calories through frequent feedings or else proteins would be utilized for energy of repair work. P
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Which one of the following does not follow the central dogma of molecular biology? 1. Pea 2. Mucor 3. Chlamydomonas 4. HIV HIV is the central dogma of molecular bi
Source of Energy and Protein The by-products obtained from grain processing (brans), oil seed processing (oil meals) and pulses processing industries are the major and importan
Computed Tomograpy Scan Developments in cross-sectional imaging techniques such as spiral tomography and reformatted computerized tomograms have become increasingly popular in
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