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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
How many regulatory enzyme types known in metabolic pathways, explain in detail by giving examples.
#question.sir please explain amoeba as in example of protozoa
Structural Features of Viruses Size of Viruses Virus particles vary widely in size. The smallest viruses e.g. virus of foot and mouth disease measuring about 20 mill mic
Water is considered a universal solvent because- Select one: a. it can dissolve polar and non-polar compounds b. it can dissolve both positively and negatively charged ions a
Role of citrate Citrate in addition to being an intermediate of citric acid cycle provides a source of acetyl CoA for the cytosolic synthesis of fatty acids. Citrate inhi
Describe how the International Astronomical Union defines a planet. Also include why Pluto is no longer considered a planet.
Define Changes in Food due to Concentration Process? Obviously concentration that exposes food to 100°C or higher temperatures for prolonged periods can cause major changes in
Explain the Transfer from liquid medium to solid media? Here the steps involved include: 1. Inoculating loop is sterilized by incineration and allowed to cool, as described
What are the special considerations required to be taken in maintenance of implants? The procedures for maintenance of patients with implants are similar to those with natural
What are the major plant tissues that form the rings observed on stem sections of some trees? The rings observed on a stem cross section of the dicot trees are made of conducti
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