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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
What ions must a plant obtain from the soil in order to make (a) ATP, (b) chlorophyll? (a) To create ATP (adenosine triphosphate) a plant requires a supply of phosphate io
Q. Investigation of aortic regurgitation by Serial Testing? Serial testing is indicated in asymptomatic patients with severe AR and preserved LV function since LV dysfunction m
Q. Treatment of angina pectoris? Proper and careful treatment of the underlying cause (usually dyslipidemia, advanced atherosclerosis or sever chronic hypertension) is imperati
What is Protein Synthesis? Protein Synthesis : Protein synthesis begins in the nucleus, with the formation of mRNA from a DNA template through transcription. The function of
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Q. Various types of microorganisms? Microbes are single-cell organisms so tiny that millions can fit into the eye of a needle. They are the oldest form of life on earth. Micro
The ideal clinical conditions of teeth which we know already include: - the absence of pain. - less than 0.1 mm initial hori-ontal mobility under lateral forces of less than
DIGESTION OF FOOD MATERIALS Digestion, you know, involves the mixing of food, its movement through the digestive tract and chemical breakdown of the large ntolecules of food
put the following events in the most probable order. A. Dead algae decomposed by bacteria B.excess nitrate and phosphate discharge into rivers C. Fish die of suffocation D.bacteria
Q. In which chloroplast structure are chlorophyll molecules found? Chlorophyll molecules are placed in an organized manner in order to improve the exposure to light on the thyl
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