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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Evaluation of the programme or agency: It can be based on following: i) Audit or review of programme or agency: This can be continuously done by the staff through in
Explain Arterial Switch Operation Surgery? This is the operation of choice for simple transposition of the great arteries as it ensures anatomical correction. The approach is t
Do moulds grow better where it is dark or light? This time leave one culture dish in a warm place where it receives light all the time. Place the other dish in a warm dark plac
Importance of Forests - Habitat and Food Forests provide habitat, and food as well as protection to wildlife species against extremes of climate and help in balancing carbon d
A glycosidic bond would be present in: Select one: a. acetone. b. methyl-alpha-D-glucose. c. 2-deoxy-beta-D-ribose. d. glucose-6-phosphate. e. fructose-1, 6-bisphosp
Q. What is the ecological importance of fungi? Fungi are decomposers and heterotrophs they break down dead beings and they actively participate in the recycling of organic mate
What is the difference between chromosomes and chromatin?
are viruses cellular organisms
Define Microscopical Examination of Starch Granules? Take a slide and make a thin film of a suspension of starch granules from different sources and examine under a microscope.
At 1:00AM, Neuron A is at rest with membrane potential equal to -70 millivolts; it is producing no action potentials. The threshold for an action potential in neuron A is -55 mill
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