Denaturing gel, Biology

Assignment Help:

Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel. 


Related Discussions:- Denaturing gel

Zygote - embryogenesis, Zygote - Embryogenesis The fertilized egg or z...

Zygote - Embryogenesis The fertilized egg or zygote is situated at the micropylar end/pole of the embryo sac, its basal (micropylar) end is attached to the embryo sac wall and

Embryology, what is the fertilization?

what is the fertilization?

Defence mechanisms of the host, Defence mechanisms of the host The hea...

Defence mechanisms of the host The healthy animal is protected from pathogenic organisms present in the environment by virtue of the effective immune mechanism present from bi

What are the differences between astral and anastral mitosis, What are the ...

What are the differences between astral and anastral mitosis? Astral mitosis is that in which there is structure of the aster, a structure made by the centrioles. Anastral mito

Results-types of surgery in ar, Results: Largely depends on the LV functi...

Results: Largely depends on the LV function. In most centres surgical mortality is 3 to 4 percent. For patients with severe LV dysfunction the immediate and late results of sur

Define the term - soil heterogeneity, Define the term - soil heterogeneity ...

Define the term - soil heterogeneity The ratings seem rather arbitrary and empirical but are meant to get a reasonable yield. Outputs for different doses of fertilizers are obt

Dormant vegetative structures, Dormant Vegetative Structures You have ...

Dormant Vegetative Structures You have learnt that light a period of chilling or application of gibberellin often breaks seed dormancy and stimulates seed germination. Vegetat

What are the major human diseases caused by roundworms, Q. What are the maj...

Q. What are the major human diseases caused by roundworms? The major human diseases caused by nematodes are ancylostomiasis, ascariasis (hookworm infection) and filariasis (com

Define the contraceptive morbidity, Define the Contraceptive Morbidity ...

Define the Contraceptive Morbidity Contraceptive morbidity, which covers any condition that result from efforts (other than abortion) to limit fertility, whether they are tradi

What is the morphological feature of molluscs, Q What is the morphological ...

Q What is the morphological feature of molluscs after which the phylum is named? The word "mollusc" imply "soft thing". Molluscs have soft bodies and this feature describes the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd