Denaturing gel, Biology

Assignment Help:

Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel. 


Related Discussions:- Denaturing gel

Post-operative care of cardiac surgical patients, POST-OPERATIVE CARE OF PA...

POST-OPERATIVE CARE OF PATIENTS The first few days following cardiac operations are the most critical in terms of the patient's survival. The safety with which a patient can

Nitrogen fixation, Nitrogen Fixation Nitrogen is an essential constitue...

Nitrogen Fixation Nitrogen is an essential constituent of living organisms and there is an inexhaustible supply of it in the atmosphere in the free form. Majority of living o

How the visual images are projected, Since the visual images are projected ...

Since the visual images are projected in an inverted manner on the retina why don't we see things upside down? As the crystalline lens is a convex spherical lens it forms inver

Nutrient availability, Nutrient Availability You have seen that cation ...

Nutrient Availability You have seen that cation exchange plays an important part in nutrient availability to plants. Cation exchange functions in two ways as nutrients are rele

Define secondary and tertiary structure of a protein, Is it expected that a...

Is it expected that a change in the primary, in the secondary or in the tertiary structure of a protein will produce more functional consequences? Any change of the protein str

Explain what is molds , Explain what is Molds ? Zygomycota, or molds, a...

Explain what is Molds ? Zygomycota, or molds, are a type of fungus made of filamentous hyphae that grow on decomposing material. The molds actually aid in the decomposition pro

Risk during crown removal-endodontics principles, Risk during crown removal...

Risk during crown removal -Tooth inadvertently extracted using a crown\bridge remover . -Endodontic therapy was performed , and the tooth was replanted , a procedure known

Difference between ectoparasite and endoparasite, Q. What is the difference...

Q. What is the difference between ectoparasite and endoparasite? The Ectoparasites are parasites that explore the external surface of the host (like, for instance, mites that p

Origin and evolution of metazoa, Origin and Evolution of Metazoa Most ...

Origin and Evolution of Metazoa Most of the early metazoans were soft bodied and so their fossils are rare. The extremely fragmented fossil record does not shed any specific l

Sulphur (s) - macronutrients, Sulphur (S) - Macronutrients Sulphur is ...

Sulphur (S) - Macronutrients Sulphur is present in the soil in inorganic and organic forms. In most soils organically bounds is the major S reservoir. The inorganic forms of S

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd