Denaturing gel, Biology

Assignment Help:

Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel. 


Related Discussions:- Denaturing gel

Explain various types of activities of a cell, The main arena of various ty...

The main arena of various types of activities of a cell is: 1. Plasma membrane 2. Mitochondrian 3. cytoplasm 4. Nucleus Cytoplasm

Define the figure of 8 and mattress suturing techniques, Define The figure ...

Define The figure of 8 and Mattress suturing techniques The figure of 8:  is placed similarly to the simple loop on the buccal aspect; however, on the lingual aspect, the needl

Define nutritional needs during pregnancy, Define Nutritional Needs during ...

Define Nutritional Needs during Pregnancy? It must be evident by now that several physiological changes occur in the body of an expectant mother and that malnutrition can' adve

Modification of mitosis, MODIFICATION OF MITOSIS 1 .      CRYPTOMITOS...

MODIFICATION OF MITOSIS 1 .      CRYPTOMITOSIS OR PROMITOSIS : Primitive type of mitosis. In this mitosis nuclear membrane not disappear (remain intact throughout the div

Eutrophication - effect on water bodies, Eutrophication - Effect on Water B...

Eutrophication - Effect on Water Bodies Moderate quantities of sewage decay naturally in a water body and water gets purified after some time. But the problem arises when the

Explain the membrane equilibria, Explain the Membrane Equilibria? A sem...

Explain the Membrane Equilibria? A semipermeable membrane used to separate two liquid phases can, in principle, be permeable to certain species and impermeable to others. A mem

Pangenesis theory - pre-mendelian theory, Pangenesis Theory (i)        ...

Pangenesis Theory (i)         Charles Darwin propounded pangenesis theory. (ii)         According to this theory every cell, of animal body produces many minute particles kn

Explain the basic working of cellular functioning, Q Which is the biologica...

Q Which is the biological molecule that holds the genetic information that is transmitted hereditarily and controls the cellular functioning? The hereditary molecule that contr

Zone of polarizing activity (zpa), Zone of Polarizing Activity (ZPA) T...

Zone of Polarizing Activity (ZPA) The A-P axis is the 1 st axis to be fixed in the presumptive limb mesoderm at a very early stage. On the basis of results of a series of cur

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd