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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
descirbe the role of allied health profession all providing pulmonary rehabilitation during treatment of emphysema
distinguish between striated and cardic muscles
A d v a nce d vaccine technology: Genetic engineering technology is the major scientific revolution of 20th Century. This has helped in developing vaccine
A patient has a low red blood cell count, and microscopic examination reveals an abnormally high proportion of circulating reticulocytes. Upon subsequent examination, the patient i
Other Complications : Bioprosthetic valves have a tendency for degeneration, calcification or cusp perforation. Current models have overcome many of these problems. Calcification
The terms ecosystem was introduction by Sir Arthur tensely in 1935. An ecosystem can be defined s " a structural and function unit of biosphere consisting of living organisms and t
prove oxygen is produced during photosynthesis in the presence of light
Q. Plant classification according to Ancient Greeks and Romans? Hippocrates, "The Father of Medicine" (460-377 B.C.) is reputed to have been one of Democritus 's disciples. He
Explain Full Liquid Diet Full Liquid Diet: This diet provides foods and fluids that are liquid or semi liquid at room temperature. The type of food provided may vary dependi
Define Conditions associated with increased need for Vitamin A? Conditions and populations associated with increased need for vitamin A includes young children particularly t
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