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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Echinococcosis (hydatidosis) Echinococcosis, also called hydatidosis, is a global problem particularly in countries where sheep and cattle raising forms the major animal husba
Primary Egg Envelopes These are the egg envelopes which develop in the ovary between oocyte and follicle cells in the space occupied by the interdigitating microvilli. Such e
How can the blood coagulation (clotting) process be described? Blood clotting encompasses a sequence of chemical reactions whose respective products are enzymes that catalyze t
What is eutrophication? Eutrophication is the process of excessive enhancing of nutrients, like phosphate and nitrate, in water because of direct deposit of non treated sewage.
are viruses cellular organisms
Fatty acids have four main biological roles: 1. They are used to create sphingolipids and glycerophospholipids which are necessary elements of biological membranes; 2. Nume
Comparing toxicity and the requirement for dilution in water how different are the ureotelic and the uricotelic excretions? What are some examples of animals that present these res
Avian adenovirus infections EDS-76, an adenovirus, has been etiologically associated with drop in egg production. Other adenoviruses affecting chicken and other birds include
Which of the following statements concerning enzymes is false? a. They function as catalysts b. The active site of an enzyme has a shape that is specific for its c. Many e
Exchange of oxygen, nutrients, carbon dioxide and waste products from mother to fetus takes place through diffusion across the: a) Chorionic villi (pron: core-e-on-ick villi)
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