Denaturing gel, Biology

Assignment Help:

Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel. 


Related Discussions:- Denaturing gel

Clinical presentation and investigation of hypertension, Any elevation in B...

Any elevation in BP is accompanied by increased risk of cardiovascular and cerebrovascular events and renal damage. The most distinctive pathological consequence

Scientific method, SCIENTIFI C METHOD - The scientific approach to exp...

SCIENTIFI C METHOD - The scientific approach to explain a particular phenomenon require a series of organized steps based on common sense. It is called scientific method. A

Stereochemistry of amino acids - optical properties, Stereochemistry of Ami...

Stereochemistry of Amino Acids: Optical Properties All  amino acids (except glycine) rotate the plane of polarized light because of  the presence of an asymmetric center at C-2

Write the meaning of hyperglycemia, Q. Write the meaning of Hyperglycemia? ...

Q. Write the meaning of Hyperglycemia? Hyperglycemia is a Greek term: hyper -meaning excessive; glyc - meaning sweet; and emia- means "of the blood". It is a condition in whic

Bjork-shiley valve (b-s)-types of valves, Bjork-Shiley Valve (B-S) : This...

Bjork-Shiley Valve (B-S) : This is one of the earliest types of tilting disc valves introduced in early 70's. The earlier models like concavo-convex disc valves were withdrawn fr

Protection of plants against toxic substances, Protection of Plants against...

Protection of Plants against Toxic Substances Plants produce several toxic substances themselves and may also encounter the ones produced by other plants or present in the env

Consequences on the bony structures, Q. Consequences on the bony structures...

Q. Consequences on the bony structures? Basal bone forms the dental skeletal structure. Wolff's law states that the bone remodels in relationship to the forces applied. With a

Serendipidity, SERENDIPIDIT Y - Sometimes discoveries are made unexpec...

SERENDIPIDIT Y - Sometimes discoveries are made unexpectedly matter of chance. This is serendipidity. Examples - 1.       Structural formula of benzene - It was dreamt by

Explain the structural and functional changes in cancer , Identify and brie...

Identify and briefly explain the structural and functional changes that occur in the large bowel when colorectal cancer develops.

Cell, what is cell and what are the components of cell

what is cell and what are the components of cell

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd