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Denaturing Gel is an agarose or the acrylamide gel run under conditions which destroy the secondary or tertiary protein or RNA. For protein, this generally means the inclusion of 2-ME (which reduces disulfide bonds amongs cysteine residues) and SDS and/or urea in the acrylamide gel. For RNA, this commonly means that the inclusion of formaldehyde or glyoxal to destroy the higher ordered RNA. In DNA sequencing gels, urea is included for denaturing dsDNA to ssDNA strands. In denaturing process of gels, macromolecules tend to be separated on the basis of size and charge, while shape and oligomerization of the molecules are not significant. Contrast with the Native Gel.
Define the Maintenance of Weight Loss? Once an individual has managed to lose weight to a desirable level, it must not be assumed that the weight loss will be maintained automa
What is the role of mitosis in growth?
Why are the recombinant DNA technology and the nucleus transplantation technology still dangerous? The recombinant DNA technology and the nucleus transplantation technology (cl
Determine the change in the amount of intracellular chloride Complete motor neuron is removed from a frog and placed in a large volume of normal physiological saline. The neur
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Divisions of Meiosis: Meiosis is characterized by two nuclear divisions, meiosis I and meiosis II. • The first division is a reduction division. • The second meiotic divis
Q. How can the binding of two amino acids for the peptide formation be explained? A peptide is formed when a carbon from the carboxyl group of one amino acid is connected to th
What is prophylaxis? Prophylaxis are measures taken to stop diseases. For instance, the use of condoms in sexual relations is a prophylaxis against contamination by agents that
Q. What is the ADP phosphorylation? What respectively are oxidative phosphorylation and photophosphorylation? ADP phosphorylation is the addition of one inorganic phosphate in
First Systematic Theory in Psychiatric Nursing: Hildegard Peplau, one of the nurse-theorist, published a book on Interpersonal Relations in Nursing (1952) and gave-the introd
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