Define ziehl-neelsen staining technique, Biology

Assignment Help:

Define Ziehl-Neelsen Staining Technique?

Ziehl-Neelsen staining is used for staining these bacteria. The technique was discovered first by Robert Koch during his pioneering investigations on tuberculosis and was the fore-runner of Ziehl-Neelsen stain used today for diagnosis of these organisms in laboratory. Acid fast bacteria are called so because once these cells get stained with basic fuchsin, these resist decolourization with acid alcohol as can be seen in margin illustiation. This acid fastness red stain is due to high lipid content particularly of mycolic acid in their cell wall. Mycolic acid, is a group of branched chain hydroxyl lipids, which bind covalently to peptidoglycan of cell wall giving the cell surface a waxy, hydrophobic consistency.


Related Discussions:- Define ziehl-neelsen staining technique

Tolerance range, organism that have wide tolerance range

organism that have wide tolerance range

Anaphase, Anaphase This  phase is of shortest duration. It  begins  wit...

Anaphase This  phase is of shortest duration. It  begins  with  a sudden  separation  of the two chromatids of each   due  to splitting   of its centromere and  then a slow mov

What is the auditory tube and its function, Q. What is the auditory tube an...

Q. What is the auditory tube and its function? Auditory tube (Eustachian tube) connects each middle ear to the throat. This tube conducts air between tympanic cavity and outsid

Biotic balance of nature, Biotic Balance of Nature A nineteenth century...

Biotic Balance of Nature A nineteenth century Biologist is reported to have expressed, Glory of Britain is due to its old maids He reasoned Healthy Britons are nourished by roa

Biochemistry, tertiary structure of protein

tertiary structure of protein

Explain the mechanisms suggested for bile acid adsorption, Explain the Mech...

Explain the Mechanisms suggested for bile acid adsorption? Mechanisms suggested for bile acid adsorption are: 1. Hydrophobic interactions between lignin and bile acids, 2

Show the value of embryology, Q. Show the value of embryology? The valu...

Q. Show the value of embryology? The value of embryology in solving taxonomic problems can be appreciated by studying some specific cases. Amongst the classical examples cited

Temperate deciduous forest - ecosystem, Temperate deciduous forest - Ecosys...

Temperate deciduous forest - Ecosystem The temperate forests are characterised by a moderate climate and broad-leafed deciduous trees, which shed their leaves in fall, are bar

Define denaturation, Denaturation: With respect to the nucleic acids, refe...

Denaturation: With respect to the nucleic acids, refers to the conversion from the double-stranded to single-stranded state, many times achieved by heating or alkaline conditions.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd