Define thin layer chromatography, Biology

Assignment Help:

Define Thin Layer Chromatography?

This is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel, alumina, cellulose, Kieselguhr, modified celluloses and polyacrylamide on glass plate or plastic sheet. This facilitates the adhesion of the adsorbent to the plate, binding agents such as calcium sulphate is incorporated into the slurry. Sometimes, the slurry is mixed with fluorescent dye for compounds to fluoresce under UV light. The method of running the chromatography is almost the same as that of paper. The advantage of TLC over paper chromatography is the time required for development is considerably less and secondly the fineness of the adsorbent powder ensures very rapid capillary flow of the solvent along the layer of adsorbent and hence considerable sharpness of the spots from original.

The methods for detecting the spots are same as paper, with an added advantage of spraying with 25% H2SO4 or keeping the plate in iodine vapours. Both the methods are useful for qualitative and quantitative purposes.


Related Discussions:- Define thin layer chromatography

Zoonoses disease-ehrlichiosis, Ehrlichiosis The disease is caused by a ric...

Ehrlichiosis The disease is caused by a rickettisia, called Ehrlichia canis. Dogs and man are the reservoir hosts. The infection is transmitted through bite of infected tick, Rhip

What are steroid hormones , Cholesterol is the precursor of the five major...

Cholesterol is the precursor of the five major classes of steroid hormones.The synthesis of steroid hormones is initiated by the removal of a six-carbon unit  from  carbon  20  of

What do you understand by open circulatory system, What do you understand b...

What do you understand by Open circulatory system? A circulatory system in which circulating fluid (blood) flows into vessels or tubes not connected to each other by small capi

Disbeliefs about insulin injection, Some people have disbeliefs regarding i...

Some people have disbeliefs regarding insulin injection. You have to find out if there are any disbeliefs and try to explain the importance of insulin injection. Do not force the p

What is the dissociation and double dissociations, Dissociation and double ...

Dissociation and double dissociations Neuropsychologists typically try to design studies that provide evidence of the differential performance of brain damaged and control subj

Proteins Requirement for nutrients, Q. Requirement for nutrients? Ulcer...

Q. Requirement for nutrients? Ulcer is a form of wound which if not healed on time call get perforated and bleed. Adequate protein intake ensures synthesis of new tissues esse

Protozoa, What is meant by encystment

What is meant by encystment

Agro industrial-evaluation of feedstuff, Evaluation of feedstuff In th...

Evaluation of feedstuff In the feed manufacturing process evaluation of the feed ingredients is very important in achieving consistent quality throughout the year. If one is a

Pyramid of biomass - ecological pyramids, Pyramid of Biomass - Ecological P...

Pyramid of Biomass - Ecological Pyramids The pyramid of numbers is not a very good method to use if the organisms at different trophic levels are of greatly differing sizes. T

Phylum, description of first five phylum

description of first five phylum

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd