Define thin layer chromatography, Biology

Assignment Help:

Define Thin Layer Chromatography?

This is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel, alumina, cellulose, Kieselguhr, modified celluloses and polyacrylamide on glass plate or plastic sheet. This facilitates the adhesion of the adsorbent to the plate, binding agents such as calcium sulphate is incorporated into the slurry. Sometimes, the slurry is mixed with fluorescent dye for compounds to fluoresce under UV light. The method of running the chromatography is almost the same as that of paper. The advantage of TLC over paper chromatography is the time required for development is considerably less and secondly the fineness of the adsorbent powder ensures very rapid capillary flow of the solvent along the layer of adsorbent and hence considerable sharpness of the spots from original.

The methods for detecting the spots are same as paper, with an added advantage of spraying with 25% H2SO4 or keeping the plate in iodine vapours. Both the methods are useful for qualitative and quantitative purposes.


Related Discussions:- Define thin layer chromatography

What is the approximate ph of the salivary secretion, What is the approxima...

What is the approximate pH of the salivary secretion? Is it an acid or basic fluid? What are the main functions of saliva? The saliva pH is approximately 6.8. It is therefore a

Why mosquito bites and it causes itching, Why mosquito bites and it causes ...

Why mosquito bites and it causes itching? A mosquito does not really bite you, of course. It sucks your blood. To help enable effective blood sucking, it firstly injects ant

Define essential parts of photocolorimeter - light source, Define Essential...

Define Essential Parts of Photocolorimeter - Light Source? The light source is usually a tungsten lamp, for wavelength in the visible range (320-700 nm) and a deuterium or hydr

Effects of smoking on oesophagitis, Q. Effects of smoking on oesophagitis? ...

Q. Effects of smoking on oesophagitis? 1. A decrease in pyloric sphincter pressure which may predispose an individual to duodenal ulcer. 2. An alteration in the nature of ga

Define drug effects on vitamin and mineral metabolism, Define Drug effects ...

Define Drug effects on Vitamin and mineral metabolism? Vitamin and mineral metabolism: Micronutrients are required as cofactors or coenzymes in many metabolic pathways, includi

What is a biologic failure, What is A biologic Failure A biologic Fail...

What is A biologic Failure A biologic Failure can be defined as the inadequacy of the host to establish or to maintain osseointegration. The inability to establish osseoint

Temperate deciduous forests, Going south of taiga you would reach the tempe...

Going south of taiga you would reach the temperate regions of the planet. These regions experience moderate temperatures on average that change during four distinct seasons. They h

Coding sequence, Coding sequence is the portion of the gene or the mRNA wh...

Coding sequence is the portion of the gene or the mRNA which actually codes for a protein. Introns are not coding for the sequences; nor are the 5' or 3' untranslated regions (or

Ecology, What is the concept of minimum and maximum way of tolerance

What is the concept of minimum and maximum way of tolerance

Hypocalcaemia, H y po c a l c a e m i a It is also known as ...

H y po c a l c a e m i a It is also known as milk fever or parturient paresis. The disease occurs due to imbalance in input and output of calcium and is characterize

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd