Define thin layer chromatography, Biology

Assignment Help:

Define Thin Layer Chromatography?

This is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel, alumina, cellulose, Kieselguhr, modified celluloses and polyacrylamide on glass plate or plastic sheet. This facilitates the adhesion of the adsorbent to the plate, binding agents such as calcium sulphate is incorporated into the slurry. Sometimes, the slurry is mixed with fluorescent dye for compounds to fluoresce under UV light. The method of running the chromatography is almost the same as that of paper. The advantage of TLC over paper chromatography is the time required for development is considerably less and secondly the fineness of the adsorbent powder ensures very rapid capillary flow of the solvent along the layer of adsorbent and hence considerable sharpness of the spots from original.

The methods for detecting the spots are same as paper, with an added advantage of spraying with 25% H2SO4 or keeping the plate in iodine vapours. Both the methods are useful for qualitative and quantitative purposes.


Related Discussions:- Define thin layer chromatography

Development and differentiation of a flowering plant, Development and Diffe...

Development and Differentiation of a flowering plant The developmental phases of a flowering plant involve seed germination, vegetative growth, flowering, fruiting and senesce

Long-term treatment of maple syrup urine disease, Q. Long-term treatment of...

Q. Long-term treatment of maple syrup urine disease? Special feeds containing energy and proteins without the branched chain amino acids can be given to the infants. In some in

Liver function test, Liver function test should be done for all diabetic pa...

Liver function test should be done for all diabetic patients. This will help the doctor in the choice of drugs for the treatment of diabetes since liver abnormalities influence the

What are the two main types of endocytosis, What are the two main types of ...

What are the two main types of endocytosis? Endocytosis can be divided as pinocytosis or phagocytosis. In pinocytosis small particles on the external surface of the membrane ex

Explain the flow phase of stress response, Explain the Flow Phase of Stress...

Explain the Flow Phase of Stress Response? This is a neuro-endocrine response to physiological stress following the ebb phase. This phase is characterized by: Normal or

Krebs cycle, Krebs Cycle The acetyl  group  of acetyl   co A  is now co...

Krebs Cycle The acetyl  group  of acetyl   co A  is now completey  degraded  stepwise  into  carbon  and hydrogen atoms  in the mitochondria  by a cyclic  sequence of  eight ma

Determine the food source for magnesium, Determine the food source for Magn...

Determine the food source for Magnesium? Magnesium is widely distributed in variety of foods and beverages. In plants it is associated with chlorophyll. Thus, green leafy veget

What are the muscle of the diaphragm, Would you expect the muscle fibers of...

Would you expect the muscle fibers of the tongue to be striated or smooth? What about the muscle of the diaphragm/ Explain your answer.

Nutritional requirement and dietary modifications in diet, Define Nutrition...

Define Nutritional Requirement and Dietary Modifications in the Diet of the Elderlu? The nutritional status influences the age-related rate of functional decline in many organ

Define methods for determining amount of microbes in food, Define Methods f...

Define Methods for Determining the Amount of Microbes in Food? Following methods can be employed for determining the amount of microbes in the food samples and analyzing their

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd