Define thin layer chromatography, Biology

Assignment Help:

Define Thin Layer Chromatography?

This is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel, alumina, cellulose, Kieselguhr, modified celluloses and polyacrylamide on glass plate or plastic sheet. This facilitates the adhesion of the adsorbent to the plate, binding agents such as calcium sulphate is incorporated into the slurry. Sometimes, the slurry is mixed with fluorescent dye for compounds to fluoresce under UV light. The method of running the chromatography is almost the same as that of paper. The advantage of TLC over paper chromatography is the time required for development is considerably less and secondly the fineness of the adsorbent powder ensures very rapid capillary flow of the solvent along the layer of adsorbent and hence considerable sharpness of the spots from original.

The methods for detecting the spots are same as paper, with an added advantage of spraying with 25% H2SO4 or keeping the plate in iodine vapours. Both the methods are useful for qualitative and quantitative purposes.


Related Discussions:- Define thin layer chromatography

What are immunoglobulins, What are immunoglobulins? Immunoglobulin is t...

What are immunoglobulins? Immunoglobulin is the exchange name given to antibody. Immunoglobulins are complex proteins having an invariable portion and a variable portion and ma

Developmental disorders, DEVELOPMENTAL DISORDERS - 1 .      AMNIONITI...

DEVELOPMENTAL DISORDERS - 1 .      AMNIONITIS- Inflammation of amnion due to infection. 2 .      ABORTION- Escape of a embryo prior to the stage of viability (ab

Name the endocytosis, Name the endocytosis?   A.  During endocytosis of...

Name the endocytosis?   A.  During endocytosis of GLUT4 transporters in fat cells, there is removal of GLUT4 transporters from plasma membranes.   B.  During endocytosis of

Define transport and uptake of zinc by cells, Define Transport and uptake o...

Define Transport and uptake of Zinc by Cells? After absorption, zinc is bound to albumin and transported to the liver. In liver, it is concentrated and then transported to diff

Why does dna twist, Why does DNA twist? If it did not twist, would you...

Why does DNA twist? If it did not twist, would you expect it to fit into the miniscule cell? As we all know, if we join all of the DNA molecules from a person's body end to en

Describe about cardiomyopathy due to persistent tachycardia, Q. Describe ab...

Q. Describe about Cardiomyopathy due to Persistent Tachycardia? In occasional cases, particularly in children recurrent or incessant episodes of supraventricular or ventricular

Determine the term poliomyelitis - brian diseases, Determine the term Polio...

Determine the term Poliomyelitis - Brian diseases Poliomyelitis is an acute infectious disease caused by a virus that has a special affinity for the motor neurons of the spinal

Explain the leydig cells, In an adult male, which of the following is true?...

In an adult male, which of the following is true? A. The plasma membranes of Leydig cells contain LH receptors. B. The plasma membranes of Sertoli cells contain FSH receptor

How to increase the levels of physical activity, How to increase the levels...

How to increase the levels of physical activity? The benefits of physical fitness should be spread among the public through mass media. 1. Physical activity should be encour

Functions of the bacterial flora within the human gut, Q. What are the majo...

Q. What are the major functions of the bacterial flora within the human gut? Bacteria that live inside the gut have great importance in digestion. Several polysaccharides like

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd