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Define Thin Layer Chromatography?
This is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel, alumina, cellulose, Kieselguhr, modified celluloses and polyacrylamide on glass plate or plastic sheet. This facilitates the adhesion of the adsorbent to the plate, binding agents such as calcium sulphate is incorporated into the slurry. Sometimes, the slurry is mixed with fluorescent dye for compounds to fluoresce under UV light. The method of running the chromatography is almost the same as that of paper. The advantage of TLC over paper chromatography is the time required for development is considerably less and secondly the fineness of the adsorbent powder ensures very rapid capillary flow of the solvent along the layer of adsorbent and hence considerable sharpness of the spots from original.
The methods for detecting the spots are same as paper, with an added advantage of spraying with 25% H2SO4 or keeping the plate in iodine vapours. Both the methods are useful for qualitative and quantitative purposes.
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Explain primary structure of proteins The primary structure of a protein having of the order in which amino acids are bonded to one another by a peptide bond.
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Tissue Valves : Tissue valves mounted on a frame with a sewing ring are inserted by the same technique as used in the case of prosthetic valves.
Define Proteins as biological buffers? Proteins have the ability to accept or donate hydrogen ions and by doing so they serve as biological buffers. In blood, there are three i
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