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Define Thin Layer Chromatography?
This is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel, alumina, cellulose, Kieselguhr, modified celluloses and polyacrylamide on glass plate or plastic sheet. This facilitates the adhesion of the adsorbent to the plate, binding agents such as calcium sulphate is incorporated into the slurry. Sometimes, the slurry is mixed with fluorescent dye for compounds to fluoresce under UV light. The method of running the chromatography is almost the same as that of paper. The advantage of TLC over paper chromatography is the time required for development is considerably less and secondly the fineness of the adsorbent powder ensures very rapid capillary flow of the solvent along the layer of adsorbent and hence considerable sharpness of the spots from original.
The methods for detecting the spots are same as paper, with an added advantage of spraying with 25% H2SO4 or keeping the plate in iodine vapours. Both the methods are useful for qualitative and quantitative purposes.
Saturated fatty acids General formula of saturated fatty acids is CH3 (CHI), COOH. It has a polar end with a free XOOH group and a non-polar end with a hydrocarbon chain, as
Agro-industrial by-products (AIBP) refer to the by-products derived due to processing of the main crop products and allied industries. They are in comparison to crop residues, are
The benefits of HACCP can be summarized as under. a) Benefits to Consumers Reduced risk of food borne diseases Increased confidence in food supply Increased awar
Some species of plant are strongly adapted to pollination by certain insects. Characteristics which are regarded as adaptations to pollination by bees are: ( a) white o
Increase in population size is known as population growth. It depends upon number of persons added to the population and number of persons lost from the population. Addition in pop
Under which forms is the Trypanosoma cruzi found in its hosts? In the definitive hosts as well as in triatomine bugs (middle hosts) the protozoan that causes Chagas' disease ch
Standardisation Research of Luria Nebraska There are published manuals for the Luria Nebraska that define the battery in detail and provide information pertinent to validity,
Why should you not apply cosmetics while doing a lab experiment in class?
Why do some trees lose their green color in the autumn? In autumn days become shorter and nights longer therefore there is a reduction of the photosynthesis rate and some plant
Explain the salient features biochemical origin of life.
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