Define thin layer chromatography, Biology

Assignment Help:

Define Thin Layer Chromatography?

This is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel, alumina, cellulose, Kieselguhr, modified celluloses and polyacrylamide on glass plate or plastic sheet. This facilitates the adhesion of the adsorbent to the plate, binding agents such as calcium sulphate is incorporated into the slurry. Sometimes, the slurry is mixed with fluorescent dye for compounds to fluoresce under UV light. The method of running the chromatography is almost the same as that of paper. The advantage of TLC over paper chromatography is the time required for development is considerably less and secondly the fineness of the adsorbent powder ensures very rapid capillary flow of the solvent along the layer of adsorbent and hence considerable sharpness of the spots from original.

The methods for detecting the spots are same as paper, with an added advantage of spraying with 25% H2SO4 or keeping the plate in iodine vapours. Both the methods are useful for qualitative and quantitative purposes.


Related Discussions:- Define thin layer chromatography

Acute bronchitis, Acute Bronchitis: Acute bronchitis is inflammation  ...

Acute Bronchitis: Acute bronchitis is inflammation  of one or more bronchi  and occurs specifically  in children younger than  four year?.  It is usually associated with previ

What is the function of the antidiuretic hormone, What is the function of t...

What is the function of the antidiuretic hormone? Where is it made and which are the stimuli that enhance or decrease its secretion? The antidiuretic hormone is secreted by the

Biotic potential of a bacterial population, Q. How different is the growth ...

Q. How different is the growth according to the biotic potential of a viral population from the growth according to the biotic potential of a bacterial population? The growth c

Suspensor - pollen biology, Suspensor - Pollen Biology The structure a...

Suspensor - Pollen Biology The structure and function of suspensor have not been given adequate attention. During the last decade, however, investigations have produced notewo

#title.zoology., #question.why obelia is considered of special interest in ...

#question.why obelia is considered of special interest in zoology.

Late recurrence of artgirza--recurrence of angina, Late recurrence of a...

Late recurrence of artgirza: This is a reflection of progress of disease in the native coronary arteries distal to the grafts or narrowing or blockage of one or more of the g

Simple proteins, SIMPL E PROTEINS The proteins are made of amino acids...

SIMPL E PROTEINS The proteins are made of amino acids only. Additional chemicals are absent. These are of two types -    (i) Fibrous        (ii) Globular ( i )

Artificial selection, ARTIFICIA L SELECTION - Man selects the indiv...

ARTIFICIA L SELECTION - Man selects the individuals having the desired traits and separates them from those, which do not possess such characters. The selected ones are

What is contact plate method - food microbiology, What is Contact Plate Met...

What is Contact Plate Method? Contact Plate (RODAC - Replicate Organism Direct Agar Contact) Method - Contact plates are special petri plates containing suitable agar media wit

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd