Define thin layer chromatography, Biology

Assignment Help:

Define Thin Layer Chromatography?

This is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel, alumina, cellulose, Kieselguhr, modified celluloses and polyacrylamide on glass plate or plastic sheet. This facilitates the adhesion of the adsorbent to the plate, binding agents such as calcium sulphate is incorporated into the slurry. Sometimes, the slurry is mixed with fluorescent dye for compounds to fluoresce under UV light. The method of running the chromatography is almost the same as that of paper. The advantage of TLC over paper chromatography is the time required for development is considerably less and secondly the fineness of the adsorbent powder ensures very rapid capillary flow of the solvent along the layer of adsorbent and hence considerable sharpness of the spots from original.

The methods for detecting the spots are same as paper, with an added advantage of spraying with 25% H2SO4 or keeping the plate in iodine vapours. Both the methods are useful for qualitative and quantitative purposes.


Related Discussions:- Define thin layer chromatography

Can you illustrate chylomicrons, Q. What is the special route that lipids f...

Q. What is the special route that lipids follow during digestion? What are chylomicrons? Triglycerides emulsified by the bile within micelles suffer the action of lipases that

Dietary guidelines fur hyperlipidemic patients, Q. Dietary Guidelines fur h...

Q. Dietary Guidelines fur hyperlipidemic patients? • Calories: to maintain ideal body weight. • Carbohydrates should constitute 55-65% of calories with emphasis on • polysacc

Symptoms of dyspepsia, Q. Symptoms of dyspepsia? The life style modific...

Q. Symptoms of dyspepsia? The life style modification in terms of work schedules, eating behaviour like consuming meals when extremely tired, quitting alcohol and smoking would

Define dietary management of sepsis with or without mods, Define Dietary Ma...

Define Dietary Management of Sepsis with or without MODS? Before we begin with the dietary management of patients suffering from sepsis with or without MODS let us read a case

What are possible treatments available, What are possible treatments availa...

What are possible treatments available for a herniated disc injury?

Contamination due to pathogens - water pollution, Contamination due to Path...

Contamination due to Pathogens - Water Pollution Sewage is a source of pathogenic viruses, bacteria, worms and other parasites. The extent of contamination by these pathogens

Use of syphilis in pregnancy, Syphilis in Pregnancy Syphilis in pregna...

Syphilis in Pregnancy Syphilis in pregnant women should be treated with penicillin in doses appropriate to the stage of the disease. When pregnant women with syphilis are alle

Reproductive system of asteroidea, Reproductive System of Asteroidea S...

Reproductive System of Asteroidea Sexes are separate in several asteroids. There are ten (10) gonads, two in each arm. They emerge tuft-like or as cluster of grapes. Mature go

How is gas exchange done in flatworms, Q. How is gas exchange done in flatw...

Q. How is gas exchange done in flatworms? Platyhelminthes exchange gases exclusively by diffusion through their body surface. This is only possible because all cells are locali

D-glucose exists in all of the forms except, As a result of mutarotation, D...

As a result of mutarotation, D-glucose exists in all of the following forms EXCEPT: Select one: a. L-glucopyranose. b. alpha-anomer. c. free aldehyde (linear) d. bet

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd