Define thin layer chromatography, Biology

Assignment Help:

Define Thin Layer Chromatography?

This is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel, alumina, cellulose, Kieselguhr, modified celluloses and polyacrylamide on glass plate or plastic sheet. This facilitates the adhesion of the adsorbent to the plate, binding agents such as calcium sulphate is incorporated into the slurry. Sometimes, the slurry is mixed with fluorescent dye for compounds to fluoresce under UV light. The method of running the chromatography is almost the same as that of paper. The advantage of TLC over paper chromatography is the time required for development is considerably less and secondly the fineness of the adsorbent powder ensures very rapid capillary flow of the solvent along the layer of adsorbent and hence considerable sharpness of the spots from original.

The methods for detecting the spots are same as paper, with an added advantage of spraying with 25% H2SO4 or keeping the plate in iodine vapours. Both the methods are useful for qualitative and quantitative purposes.


Related Discussions:- Define thin layer chromatography

Food and digestion, what is the pH inside the stomach?Why is there a need t...

what is the pH inside the stomach?Why is there a need to keep that pH level? How is it maintained?

Potassium ion can pass easily through cell membranes, In the human body, th...

In the human body, the potassium ion can pass easily through cell membranes, yet the potassium ion concentration is higher inside many cells than it is outside these cells. Could t

What is mitosis, What is mitosis? What is the importance of mitosis? Mi...

What is mitosis? What is the importance of mitosis? Mitosis is the process in which one eukaryotic cell separates into two cells identical to the parent cell (generally identic

Explain the bioavailability of nicotinic acid, Explain the Bioavailability ...

Explain the Bioavailability of Nicotinic Acid? We have already learnt earlier that niacin is provided in the diet primarily as the pyridine nucleotides-NAD and NADP. In additio

Taxonomy, Agroup of realated genera are classified as-?

Agroup of realated genera are classified as-?

Etiologial microorganisms, Viridans Streptococci These streptococci, whi...

Viridans Streptococci These streptococci, which cause 30 to 65 per cent of NVE case unrelated to drug abuse, are normal inhabitants of the oropharynx, characteristically produce

What is the name of the larval stage of polychaetes, Q. Is the embryonic de...

Q. Is the embryonic development in earthworms indirect or direct? The embryonic development is direct because in earthworms there is no larval stage. Q. What is the name of

What are the techniques of rastelli operations, What are the Techniques of ...

What are the Techniques of rastelli operations ? After median sternotomy pericardial patch is harvested and then cardiopulmonary bypass instituted using aortic and bicaval cann

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd