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Define Thin Layer Chromatography?
This is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel, alumina, cellulose, Kieselguhr, modified celluloses and polyacrylamide on glass plate or plastic sheet. This facilitates the adhesion of the adsorbent to the plate, binding agents such as calcium sulphate is incorporated into the slurry. Sometimes, the slurry is mixed with fluorescent dye for compounds to fluoresce under UV light. The method of running the chromatography is almost the same as that of paper. The advantage of TLC over paper chromatography is the time required for development is considerably less and secondly the fineness of the adsorbent powder ensures very rapid capillary flow of the solvent along the layer of adsorbent and hence considerable sharpness of the spots from original.
The methods for detecting the spots are same as paper, with an added advantage of spraying with 25% H2SO4 or keeping the plate in iodine vapours. Both the methods are useful for qualitative and quantitative purposes.
Explain the Dietary Management of Sepsis and MODS? Patients suffering from sepsis and/or resultant multiple system organ dysfunctions are critically ill and admitted in the int
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Syphilis in Pregnancy Syphilis in pregnant women should be treated with penicillin in doses appropriate to the stage of the disease. When pregnant women with syphilis are alle
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Degrade a monoglyceride that has an 18-carbon fatty acid attached to it by Ester bonds. You will have to degrade the glycerol component followed by the fatty acid in presence of O2
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Rituals: The social life of the earliest human groups or tribes revolved around food gathering. To begin with, they must have collected anything they could eat-seeds, nuts, f
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