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Define Thin Layer Chromatography?
This is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel, alumina, cellulose, Kieselguhr, modified celluloses and polyacrylamide on glass plate or plastic sheet. This facilitates the adhesion of the adsorbent to the plate, binding agents such as calcium sulphate is incorporated into the slurry. Sometimes, the slurry is mixed with fluorescent dye for compounds to fluoresce under UV light. The method of running the chromatography is almost the same as that of paper. The advantage of TLC over paper chromatography is the time required for development is considerably less and secondly the fineness of the adsorbent powder ensures very rapid capillary flow of the solvent along the layer of adsorbent and hence considerable sharpness of the spots from original.
The methods for detecting the spots are same as paper, with an added advantage of spraying with 25% H2SO4 or keeping the plate in iodine vapours. Both the methods are useful for qualitative and quantitative purposes.
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The dietary principles underlying the enteric diet include: High calorie High protein High carbohydrate Moderate fat High fluid Low fibre and soft diet
CL A W S - In rabbit and other animals claws present, made up of keratine or S. corneum. Dorsal plate is unguise & ventral plate is sub unguise . Helpful in digging
lest the characteristics of Protozoa
Question 1: Show applications of Bioinformatics in pharmacogenomics. Expand FASTA and BLAST. List out any 10 applications of Bioinformatics in pharmacogenomics Expand
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Q. Protein Requirement during congestive cardiac failure? Protein: The protein requirements remain the &me as healthy adult men and women, About 0.8 - lg of protein per kg
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