Define thin layer chromatography, Biology

Assignment Help:

Define Thin Layer Chromatography?

This is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel, alumina, cellulose, Kieselguhr, modified celluloses and polyacrylamide on glass plate or plastic sheet. This facilitates the adhesion of the adsorbent to the plate, binding agents such as calcium sulphate is incorporated into the slurry. Sometimes, the slurry is mixed with fluorescent dye for compounds to fluoresce under UV light. The method of running the chromatography is almost the same as that of paper. The advantage of TLC over paper chromatography is the time required for development is considerably less and secondly the fineness of the adsorbent powder ensures very rapid capillary flow of the solvent along the layer of adsorbent and hence considerable sharpness of the spots from original.

The methods for detecting the spots are same as paper, with an added advantage of spraying with 25% H2SO4 or keeping the plate in iodine vapours. Both the methods are useful for qualitative and quantitative purposes.


Related Discussions:- Define thin layer chromatography

Pyramid of numbers - ecological pyramids, Pyramid of Numbers - Ecological P...

Pyramid of Numbers - Ecological Pyramids A graphic representation of the total number of individuals of different species belonging to each trophic level in an ecosystem is kn

Explain the importance of the auxin, a) How is the milk production regulate...

a) How is the milk production regulated by hormones in human female? Define. b) Explain the importance of the auxin / cytokinin ratio in plant tissue culture.

Sutures are also classified by thread diameter, Sutures are also classified...

Sutures are also classified by thread diameter: Thread materials range in diameter from 1 to 10 with the higher number corresponding to the thinner, more delicate thread. In

Define higher order structure, Which kind of weak interaction can define hi...

Which kind of weak interaction can define higher order structure and in the same time DNA.

Define regulation of malice enzyme by starvation & refeeding, Define regula...

Define regulation of malice enzyme by starvation & refeeding? ME catalyzes the oxidative decarboxylation of malate to pyruvate and CO 2 simultaneously generating the reduced

Kingdom animalia, critera for the animal classification

critera for the animal classification

Muscle cells, explain why it is essential that muscle cells, during anaerob...

explain why it is essential that muscle cells, during anaerobic respiration, convert pyruvic acid(pyruvate) into lactic acid, even though the conversion results in an accumulation

Central carbons participate in the union between amino acids, Q. Do the -R ...

Q. Do the -R groups bound to the central carbons participate in the union between amino acids? The peptide bond attaches the nitrogen of the amine group of one amino acid to th

Name the group of fungi which belong to fermentation, Truffles, morels and ...

Truffles, morels and the many yeasts that are basic to fermentation processes belong to this group of fungi. These fung are termed as: a) Phycomycetes b) Ascomycetes c)

What are antioxidants, What are antioxidants? To put it simply, antioxi...

What are antioxidants? To put it simply, antioxidants are important naturally occurring nutrients, (vitamins, minerals) which help to protect body from certain types of cancers

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd