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Define Thin Layer Chromatography?
This is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel, alumina, cellulose, Kieselguhr, modified celluloses and polyacrylamide on glass plate or plastic sheet. This facilitates the adhesion of the adsorbent to the plate, binding agents such as calcium sulphate is incorporated into the slurry. Sometimes, the slurry is mixed with fluorescent dye for compounds to fluoresce under UV light. The method of running the chromatography is almost the same as that of paper. The advantage of TLC over paper chromatography is the time required for development is considerably less and secondly the fineness of the adsorbent powder ensures very rapid capillary flow of the solvent along the layer of adsorbent and hence considerable sharpness of the spots from original.
The methods for detecting the spots are same as paper, with an added advantage of spraying with 25% H2SO4 or keeping the plate in iodine vapours. Both the methods are useful for qualitative and quantitative purposes.
Explain lipids? Lipids : Lipids function as energy-storing molecules such as fats and oils, protective waxes, digestive tract lubricants, heat insulation such as whale blubber
Andalusian chickens may be either black, white, or gray. The gene for black is not dominant over the gene for white, nor is the gene for white dominant over the gene for black. Whe
Shell - body form Protozoans Majority of protozoans posses an exoskeleton in the form of a shell, also called test. The shell forms a loose covering around the body of the ani
Some ecosystems possess relatively large number of species as compared to others. This is particularly true of tropical forests which exhibit a very rich-biodiversity. For example,
15 character of protozoa
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Two gene loci, A and B, assort independently, and alleles A and B are dominant over alleles a and b. Indicate the probability of producing: An aB phenotype from a cross AaBb x AaBB
Researchers (Helle et al., 2004) analyzed rates of twin births in the Sami population of Northern Scandinavia during the eighteenth and nineteenth centuries. They found that (1) a
Q. What is the lasting form in the gametophyte, pteridophytes or the sporophyte? How can it be compared to bryophytes? The lasting form in pteridophytes is the diploid (2n) spo
two types of amoebas waste and how they get rid of them
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