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Define Thin Layer Chromatography?
This is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel, alumina, cellulose, Kieselguhr, modified celluloses and polyacrylamide on glass plate or plastic sheet. This facilitates the adhesion of the adsorbent to the plate, binding agents such as calcium sulphate is incorporated into the slurry. Sometimes, the slurry is mixed with fluorescent dye for compounds to fluoresce under UV light. The method of running the chromatography is almost the same as that of paper. The advantage of TLC over paper chromatography is the time required for development is considerably less and secondly the fineness of the adsorbent powder ensures very rapid capillary flow of the solvent along the layer of adsorbent and hence considerable sharpness of the spots from original.
The methods for detecting the spots are same as paper, with an added advantage of spraying with 25% H2SO4 or keeping the plate in iodine vapours. Both the methods are useful for qualitative and quantitative purposes.
T r ad i tional Uses of dung Dung has an astonishing and myriad variety of uses which have been developed over a period of time. Dung patties ( gootee ) are mainly used by
The quartenary structure of a holoenzyme contains four long polypeptide subunits composed of non-polar, polar and charged amino acids. All subunits are properly folded and function
What is Micro-Endodontic Surgery? Combines of the magnification and illumination provided by the microscope with proper use of new micro-instruments.
When the Balance Sheet column totals don't agree on the first attempt work backward through the process used in preparing the work sheet exclusively take the following steps until
sanguivarous mode of feeding in annelida
Carbon paper leaf prints Cover the vein side of a leaf with a very thin layer of lard or vaseline. Place the greased leaf vein side up on various layers of newspaper and cover
Define the Process of Digestion of Fats? The enzyme in human gut which is responsible for fat digestion is 'lipase', Lipase is secreted by both stomach and pancreas. In stomach
Explain Procedure for Capsule Staining in a Culture? We have learnt that Capsule staining can be undertaken either by negative staining or Anthony method. Now carry out the exe
Exon are those parts of a genomic DNA sequence which will be represented in final, mature mRNA t A contiguous segment of the genomic DNA which codes for a polypeptide in the gene.
What are the similarities between chlorplasts and prokaryotic cells?
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