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Define Thin Layer Chromatography?
This is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel, alumina, cellulose, Kieselguhr, modified celluloses and polyacrylamide on glass plate or plastic sheet. This facilitates the adhesion of the adsorbent to the plate, binding agents such as calcium sulphate is incorporated into the slurry. Sometimes, the slurry is mixed with fluorescent dye for compounds to fluoresce under UV light. The method of running the chromatography is almost the same as that of paper. The advantage of TLC over paper chromatography is the time required for development is considerably less and secondly the fineness of the adsorbent powder ensures very rapid capillary flow of the solvent along the layer of adsorbent and hence considerable sharpness of the spots from original.
The methods for detecting the spots are same as paper, with an added advantage of spraying with 25% H2SO4 or keeping the plate in iodine vapours. Both the methods are useful for qualitative and quantitative purposes.
Oxidised starch Oxidised starch finds a number of uses in the food industry where a neutral tasting, low viscosity 'body builder' is required as in lemon curd manufacture, in s
Define Advantages of using Bacteria as a source of protein? Advantages of using Bacteria as a source of protein are as follow: 1. High yield of protein (60-80%) 2.
Ovule The ovule, also known as the megasporangium is the forerunner of the seed. It consists of a central mound of tissue called the nucellus, which is enveloped by one or tw
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R e troviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transf
Deoxyribonucleic Acid (DNA) - polymer of nucleotides containing genetic information that codes for proteins Nucleotide - a monomer of DNA consisting of a ribose/deoxyribose sug
Chelating agents Oxalates: Only a few plants contain sufficient amounts of sodium and potassium oxalate to be considered toxic. Moreover, ruminants that consume these plants
Results of CABG: In most centres where this operation is routinely conducted the mortality is 1-2 per cent. In high-risk group of patients this may go up to 5 to 10 per cent.
Double Vessel Disease (DVD) : Percutaneous intervention with stetting is usually advised for these patients. However if it is left main equivalent disease or when block is close
Q. Describe Induced Ventricular Arrhythmias? The prognostic significance of ventricular ectopy provoked by stress testing after an MI is controversial. Complex ventricular arrh
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