Define thin layer chromatography, Biology

Assignment Help:

Define Thin Layer Chromatography?

This is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel, alumina, cellulose, Kieselguhr, modified celluloses and polyacrylamide on glass plate or plastic sheet. This facilitates the adhesion of the adsorbent to the plate, binding agents such as calcium sulphate is incorporated into the slurry. Sometimes, the slurry is mixed with fluorescent dye for compounds to fluoresce under UV light. The method of running the chromatography is almost the same as that of paper. The advantage of TLC over paper chromatography is the time required for development is considerably less and secondly the fineness of the adsorbent powder ensures very rapid capillary flow of the solvent along the layer of adsorbent and hence considerable sharpness of the spots from original.

The methods for detecting the spots are same as paper, with an added advantage of spraying with 25% H2SO4 or keeping the plate in iodine vapours. Both the methods are useful for qualitative and quantitative purposes.


Related Discussions:- Define thin layer chromatography

How is the lac operon regulated by the sigma factor, 1. Many bacterial gene...

1. Many bacterial genes show adaptive regulation of their transcription. a) How is the lac operon regulated by the sigma factor? b) How is the lac operon regulated by lactose

Define tubular-dorsal nervous system, Q. How can the tubular-dorsal nervous...

Q. How can the tubular-dorsal nervous system in chordates be compared to the nervous pattern present in invertebrates? In chordates the nervous system is dorsal and highly ceph

What is the rh factor, What is the Rh factor? The RH factor is a protei...

What is the Rh factor? The RH factor is a protein of the red blood cell plasma membrane that behaves as antigen in blood transfusions triggering a humoral (antibody-based) immu

Explain H - shaped incision, Q. Explain H - Shaped Incision? This type ...

Q. Explain H - Shaped Incision? This type of incision is especially useful for: The anterior maxilla or mandible. Accurately identified implant position. Exposure o

What is lyophobic, What is lyophobic If the affinity of the dispersed p...

What is lyophobic If the affinity of the dispersed phase to go into or to remain in colloidal dispersion is slight, the dispersed phase is said to be lyophobic (solvent repelli

MEGASPOROGENESIS, WHAT IS MEGASPOROGENESIS?wHERE IT OCCUR?hOW IT OCCURS?

WHAT IS MEGASPOROGENESIS?wHERE IT OCCUR?hOW IT OCCURS?

Procedures for diagnosis - restriction enzyme analysis, R e striction...

R e striction enzyme analysis: The restriction endonuclease analysis of viral DNA fragments or DNA fingerprinting discovered in 1970s has revolutionized the study o

Delimitation and limitation, Delimitation and Limitation: There may  b...

Delimitation and Limitation: There may  be many  aspects of the problem that need  to be  explored, but  it  is difficult to  cover all aspects in  a single research study bec

Excretion, what are the excretory organs of birds

what are the excretory organs of birds

Organisms contain enzymes, Some organisms contain enzymes that condense a f...

Some organisms contain enzymes that condense a fatty alcohol and a fatty acid to generate wax esters. Draw decanol, plamitic acid (C16:0 fatty acid) and the resulting wax ester gen

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd