Define thin layer chromatography, Biology

Assignment Help:

Define Thin Layer Chromatography?

This is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel, alumina, cellulose, Kieselguhr, modified celluloses and polyacrylamide on glass plate or plastic sheet. This facilitates the adhesion of the adsorbent to the plate, binding agents such as calcium sulphate is incorporated into the slurry. Sometimes, the slurry is mixed with fluorescent dye for compounds to fluoresce under UV light. The method of running the chromatography is almost the same as that of paper. The advantage of TLC over paper chromatography is the time required for development is considerably less and secondly the fineness of the adsorbent powder ensures very rapid capillary flow of the solvent along the layer of adsorbent and hence considerable sharpness of the spots from original.

The methods for detecting the spots are same as paper, with an added advantage of spraying with 25% H2SO4 or keeping the plate in iodine vapours. Both the methods are useful for qualitative and quantitative purposes.


Related Discussions:- Define thin layer chromatography

Why degree of dispersion of protein solution is decreased, Degree of disper...

Degree of dispersion of protein solution is decreased. It is decreased because of certain reasons:- Association: refers to changes occuring at subunit or molecular leve

Define vegetable as a rich source of protein, Define Vegetable as a rich so...

Define Vegetable as a rich source of protein? Fresh vegetables are not considered to be a very good source of proteins. On fresh weight basis, the average protein content of so

DNA, where does the free nucleotides present in the nucleus come from

where does the free nucleotides present in the nucleus come from

Differences and similarities between nematodes and annelids, Q. What are th...

Q. What are the morphological differences and similarities between nematodes and annelids? Nematodes, like annelids, have a cylindrical elongated body. Annelids differentiate f

What are cilia and flagella, What are cilia and flagella? Cilia and fla...

What are cilia and flagella? Cilia and flagella are structures found in prokaryotes as well in eukaryotic cells. They play defense, nutrition and movement roles for the cell. I

Explain about the protein isolates, Explain about the Protein Isolates? ...

Explain about the Protein Isolates? Before we begin our discussion on protein isolates, let us first get to know how protein concentrates differ from isolates. Basically, the t

Types of ecosystem, Ecosystems are so varied in form and structure that wha...

Ecosystems are so varied in form and structure that whatever has a distinct community of its own, can be called an ecosystem eg. A crop field, grass land, barks etc. all the ecosys

Endocrine glands of an insect, ENDOCRINE GLANDS OF AN INSECT - The endo...

ENDOCRINE GLANDS OF AN INSECT - The endocrine glands of cockroach are as follows. 1.       Intercerebral gland cells. These cells lie in the brain. They secrete brain horm

Define carcinogenic - dietary factors, Define Carcinogenic - Dietary Factor...

Define Carcinogenic - Dietary Factors? Dietary constituents can also be carcinogenic. But to what extent diet is one of the contributing factors to cancer development is not kn

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd