Define thin layer chromatography, Biology

Assignment Help:

Define Thin Layer Chromatography?

This is an improvement over paper chromatography and uses thin layers of slurried absorbents like silica gel, alumina, cellulose, Kieselguhr, modified celluloses and polyacrylamide on glass plate or plastic sheet. This facilitates the adhesion of the adsorbent to the plate, binding agents such as calcium sulphate is incorporated into the slurry. Sometimes, the slurry is mixed with fluorescent dye for compounds to fluoresce under UV light. The method of running the chromatography is almost the same as that of paper. The advantage of TLC over paper chromatography is the time required for development is considerably less and secondly the fineness of the adsorbent powder ensures very rapid capillary flow of the solvent along the layer of adsorbent and hence considerable sharpness of the spots from original.

The methods for detecting the spots are same as paper, with an added advantage of spraying with 25% H2SO4 or keeping the plate in iodine vapours. Both the methods are useful for qualitative and quantitative purposes.


Related Discussions:- Define thin layer chromatography

What do you understand by pharynx, What do you understand by Pharynx? T...

What do you understand by Pharynx? The region of digestive tract between the mouth and esophagus. In most animals it's muscular and forces food into the digestive tract which l

How hermaphrodite species present cross-fecundation, Is it possible for a h...

Is it possible for a hermaphrodite species to present cross-fecundation? There are hermaphrodite species of animals and plants that present cross-fecundation mainly because of

Nutritional management of food allergies & food intolerance, Define Nutriti...

Define Nutritional Management of Food Allergies and Food intolerance? In this unit we will be discussing the adverse reactions associated with food. You may have read or heard

What is the difference among dna and rna, Concerning the nitrogen-containin...

Concerning the nitrogen-containing bases that participate in nucleotides, what is the difference among DNA and RNA? In DNA nucleotides can be formed of adenine (A), thymine (T)

What are the disadvantages of mucoperiosteal flap, What are the Disadvantag...

What are the Disadvantages of mucoperiosteal flap More traumatic - flap is raised  Sutures are required and the next procedure of impression is delayed till afterthe suture

Prodigality of nature, By prodigality of nature it is meant that organisms ...

By prodigality of nature it is meant that organisms have an enormous potential to reproduce. A carp or salmon is known to lay over a million eggs. A frog may lay Bs many as 12,000

Optical rotation in organice compounds, how the optical rotaion occurs in g...

how the optical rotaion occurs in glucose and ribose?

Show the model of behaviour change, Behavior Change Communication (BCC) int...

Behavior Change Communication (BCC) interventions that address diabetes mellitus are designed to promote behaviors that prevent diabetes and complications of diabetes. Behavior cha

To study if bacteria grow better where it is warm or cold, To study if bact...

To study if bacteria grow better where it is warm or cold Again inoculate two sterile dishes. Label one 'warm'  and the other 'cold'. Place the 1st dish in a dark warm place an

Describe briefly about vitamin B2, Vitamin B 2 (Riboflavin) Riboflavin...

Vitamin B 2 (Riboflavin) Riboflavin is a yellow to orange-yellow, crystalline powder of faint odour and intensely bitter taste. Its solubility in water is very slight and depe

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd