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Define the Utilization of Most Probable Number Techniques?
The MPN technique (multiple tube technique) can be used to determine the presumptive coliform count in the food. The sample is homogenized adequately and decimally diluted in saline solution or peptone water. Measured portion (e.g. 1 ml) of the solution and dilutions is transferred into multiple tubes (3 or 5) of selective and differential medium e.g. LST and incubated at 35°C for 48 hours.
Production of gas in inverted Durham tube by lactose fermentation indicates coliforms. By using standard MPN tables, as given herewith, MPN/ml or MPN/g of food samples is derived from number of positive tubes at 3 successive dilutions.The method was given by Mc Crady in 1915 and is statistically based. Three tubes or five tubes method can be used by inoculating 3 serial dilution of the sample into 9 or 15 tubes of appropriate medium respectively. The number of organisms in the original sample is determined by using standard MPN table as we have already studied in the last practical.
Determination of Limb Polarities The limb pattern is characterized by three axes (or polarities) as displayed in diagram: (1) The direction from the shoulder (or hip) to t
Q. What is Tyrosinemia? There are two forms of hereditary tyrosinemia. They are tyrosinemia Type I and tyrosinemia Type II. Type I was thought to be due to a deficiency of para
A molecule that acts by transferring a phosphate group from an ATP molecule to another molecule would be called: A) hydrolase. B) lipase. C) transferase. D) ligase. E) kinase.
NERV E FIBRES - Axon or dendrite of a nerve cell covered with one, two or three sheaths is called nerve fibre. Dendrites are surrounded only by one sheath. An axon may b
Mountains and their forest ecosystems are regarded as the water towers of the world. A good example is the extraordinarily massive Himalayan Mountains which have shaped the climate
what are the disadvantages of protozoa?
Describe TR Murmur and VSD murmur in details? TR Murmur : Characteristic: Holosystolic murmur, loudest at left lower-sternal border and becomes louder with inspiration (Carv
Strawberry Slain: It signfies the dilatation of deeper vessels to form cavernous spaces like those seen in the erectile tissue of the penis. The lesion is well-defined
What is Photosynthesis ? Photosynthesis is the method by which plants trap radiant energy from the sun and convert the energy into a biochemical form. This biochemical energy
harmful and beneficial usesa of phylum porifera
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