Define the utilization of most probable number techniques, Biology

Assignment Help:

Define the Utilization of Most Probable Number Techniques?

The MPN technique (multiple tube technique) can be used to determine the presumptive coliform count in the food. The sample is homogenized adequately and decimally diluted in saline solution or peptone water. Measured portion (e.g. 1 ml) of the solution and dilutions is transferred into multiple tubes (3 or 5) of selective and differential medium e.g. LST and incubated at 35°C for 48 hours.

Production of gas in inverted Durham tube by lactose fermentation indicates coliforms. By using standard MPN tables, as given herewith, MPN/ml or MPN/g of food samples is derived from number of positive tubes at 3 successive dilutions.The method was given by Mc Crady in 1915 and is statistically based. Three tubes or five tubes method can be used by inoculating 3 serial dilution of the sample into 9 or 15 tubes of appropriate medium respectively. The number of organisms in the original sample is determined by using standard MPN table as we have already studied in the last practical.


Related Discussions:- Define the utilization of most probable number techniques

Cutaneous respiration in earthworm., Cutaneous respiration in earthworm. ...

Cutaneous respiration in earthworm. The respiration performed by the skin is called cutaneous respiration  In earthworm skin is the only organ of respiration. The skin

Heart and lungs, what are the main functions of lungs and heart

what are the main functions of lungs and heart

Explain the features of alternaria, Explain the Features of Alternaria? ...

Explain the Features of Alternaria? 1. Alternaria colony is wooly and compact. Underside is very dark coloured. Colony colour is grayish green or black with gray edges rapidly

What is the production of the rna, Q. What is the production of the RNA cal...

Q. What is the production of the RNA called and what is the enzyme that catalyzes the procedure? The making of the RNA from information contained in the DNA is called transcrip

Does thermal inversion occur in the winter or in the summer, Does thermal i...

Does thermal inversion occur in the winter or in the summer? Pollutant low altitude thermal inversion happens in the winter. In this period of the year the sun heats the soil l

Dietary management during congestive cardiac failure, Q. Dietary Management...

Q. Dietary Management during congestive cardiac failure? Nutritional care is a little difficult in congestive cardiac failure. This is because oedema complicates the nutritiona

Intermediate products of anaerobic respiration, (a) What are the intermedia...

(a) What are the intermediate products of anaerobic respiration in an active muscle? (b) Which of them is associated with oxygen debt? (c) In what way is th

Porifera, method of digestion in porifera

method of digestion in porifera

Explain fats, Explain Fats Fats: As you are already aware,  fats are ...

Explain Fats Fats: As you are already aware,  fats are reservoir  of  calories. In  addition  to carbohydrates,  fats can  also be  an important  source of  needed calories.

Explain how will you perform ward radiography?, Question 1 Explain how ...

Question 1 Explain how will you perform ward radiography? Question 2 Discuss the radiographic projections recommended for imaging the first to third cervical vertebr

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd