Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define the Streak Plate Method - Pure Culture Techniques?
The method was developed by Loeffler and Gaffkey in the laboratory of Robert Koch. The streak plate method depends on spatial separation of single cells. The mixed microbial culture is transferred to the edge of an agar plate and then a series of parallel non-overlapping streaks are made in some specific pattern over the surface of the nutrient medium with the help of inoculating loop.
As the microbes are rubbed off the loop on to the medium, there is a continuous reduction in the number of microbes till the last cells to be rubbed off the loop are far enough apart to form isolated/ discrete colonies. The isolated colony can be picked and restreaked on nutrient agar plate to get a pure culture. Streak plate method is the most commonly used method to isolate pure cultures. As discussed above, principle of streak plate method is continuous dilution of the microbes, resulting in separation of individual cells. These cells then form isolated colonies.
Repeated picking and restreaking of isolated colony ultimately results in a pure culture. Different patterns can be used for streaking. Common ones are quadrant (four way streaks) and full plate streak methods. There are other variants of streaking pattern also.The method works well when the organism to be isolated is present in large amounts in a mixture. However, when the amount of desired organism is less, its level has to be increased by using specific enrichment culture before performing streaking.
DISORDE R RELATED TO NUTRITION AND DIGESTIVE SYSTEM Malnutrition Malnutrition is improper intake of food. Malnutrion covers both undernutrition and overnutrition. Und
Explain the term Brown-Sequard Syndrome Brown-Sequard syndrome is a rare spinal disorder that results from an injury to one side of the spinal cord in which the spinal cord is
DIFFERENCES BETWEEN PROKARYOTIC & EUKARYOTIC CELLS S . N o . CHA R AC T ERS P R O K AR Y O T I C CELL
Radiant Warmer A radiant warmer is a device used to maintain the body temperature of the newborn and thus play an essential role in influencing oxygen consumption, apnea and
In biology, a species is smallest and basic unit of classification. Each species is placed within a single genus. For example biological name of human being is homosapiens. Here ho
What is the significance of Pronephric? The first, or ancestral, kidney that appears in the anterior part of the coelomic cavity and is connected to the archinephric duct. In a
Q. Illustrate Physiological Evidence ? Physiological and biochemical evidence is providing data of increasing importance to plant systematic. Recently, it has become apparent t
Catastrophism Once-popular belief which events in the history of earth had occurred in the past a sudden events and by processes different from those operating today. The periods
Explain the Photosensitive Detectors? A wide range of detectors varying in senstivity and cost are available. They contain a light sensitive surface that releases electrons in
CONCLUSION OF PHYLUM ANNELIDA
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd