Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define the Principle behind Cyanmethemoglobin Method?
The principle behind the cyanmethemoglobin method is as follow:
Principle The haemoglobin is treated with a reagent containing potassium cyanide and sodium carbonate. With haemoglobin ferricyanide forms methaemoglobin which is converted to cyanmethaemoglobin or haemiglobincyanide (HiCN) with cyanide. The absorbance is measured at 540 nm. The reaction is presented herewith:
Haemoglobin + potassium ferricyanide ------→ Methaemoglobin
Methaemoglobin + potassium cyanide ------→ Cyanmethaemoglobin (HiCN)
in solution the ferrous ions of the haemoglobin are oxidized to the ferric state by potassium fericyanide to form methaemoglobin. In turn methaemoglobin reacts with cyanide ions (CN-) provided by potassium cyanide to form HiCN. The time required for full colour development is shortened by if dihydrogen potassium phosphate is substituted for sodium bicarbonate in the classic Drabkin's reagent. The colour is stable for more than 24 hours in well stoppered tubes kept in dark. An HiCN standard can be used to calibrate the spectrophotometer, The concentration of HiCN in the sample is calculated from the absorbance in a calibrated spectrophotometer or alternatively with the help of a standard curve.
The terms ecosystem was introduction by Sir Arthur tensely in 1935. An ecosystem can be defined s " a structural and function unit of biosphere consisting of living organisms and t
Explain The nutritional care process The nutritional care process is a systematic and logical approach to ensure effective and successful nutrition and intervention. The Ame
Q. What are the most important structures within the cell nucleus? Within the cell nucleus the most important structures are the nucleolus, an optically dense region, spherical
Define the Reaction to Food Additives? Food additives such as preservatives, flavour enhancers and colouring agents have been linked to adverse food reactions. Additives implic
In one study of a heavily polluted area near Birmingham, England, a researcher observed a frequency of 87% melanic Biston betlularia. Calculate the frequency of the dominant allele
Disease Y Study: Let us consider the Disease Y study, a brief description of the study is as follows. A case-control study was conducted of 75 cases with a rare genetic disorder "
How is the responsible slromal swelling pressure for corneal hydration? Slromal Swelling Pressure Stromal Swelling Pressure (SSP) is the pressure exerted by GAG (Glycosa
A mother has brought her 2-week-old infant to the emergency department due to the baby's persistent and increasing jaundice. Blood testing reveals that the infant's unconjugated bi
The different organs of the gastrointestinal system Mouth or buccal cavity - Teeth 32 Permanent and - Tongue Pharynx Oesophagus Stomach Small Intestine
Q. What about the fluid and other nutrient intake during constipation? The fluid intake should be at least 2 litres daily. This includes fluid as foods and beverage besides wa
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd