Define the principle behind cyanmethemoglobin method, Biology

Assignment Help:

Define the Principle behind Cyanmethemoglobin Method?

The principle behind the cyanmethemoglobin method is as follow:

Principle The haemoglobin is treated with a reagent containing potassium cyanide and sodium carbonate. With haemoglobin ferricyanide forms methaemoglobin which is converted to cyanmethaemoglobin or haemiglobincyanide (HiCN) with cyanide. The absorbance is measured at 540 nm. The reaction is presented herewith:

Haemoglobin + potassium ferricyanide ------→ Methaemoglobin

Methaemoglobin + potassium cyanide ------→ Cyanmethaemoglobin (HiCN)

in solution the ferrous ions of the haemoglobin are oxidized to the ferric state by potassium fericyanide to form methaemoglobin. In turn methaemoglobin reacts with cyanide ions (CN-) provided by potassium cyanide to form HiCN. The time required for full colour development is shortened by if dihydrogen potassium phosphate is substituted for sodium bicarbonate in the classic Drabkin's reagent. The colour is stable for more than 24 hours in well stoppered tubes kept in dark. An HiCN standard can be used to calibrate the spectrophotometer, The concentration of HiCN in the sample is calculated from the absorbance in a calibrated spectrophotometer or alternatively with the help of a standard curve.


Related Discussions:- Define the principle behind cyanmethemoglobin method

What are the main cellular functions of potassium, What are the main cellul...

What are the main cellular functions of potassium? Besides being significant for the osmotic regulation and for the acid-base equilibrium (pH) potassium is basic for the excita

Define importance of body composition assessment in athletes, Define Import...

Define Importance of Body composition assessment in athletes? Body composition and weight are two of the many factors that contribute to optimal exercise performance. Body weig

What are similarities between reptiles and birds, Q. What similarities do r...

Q. What similarities do reptiles and birds share regarding external coverage, reproduction and excretion? Regarding external coverage, birds are similar to reptiles as they pre

How have brain-imaging methods such as pet and mri, How have brain-imaging ...

How have brain-imaging methods such as PET and MRI affected neuroscience research and clinical care? Techniques which capture pictures of the living human brain at work have be

Solid waste management, All Solid Semi-Solid Wastes Arising From Human And ...

All Solid Semi-Solid Wastes Arising From Human And Animals Activities, Except Human Excreta And Sullage (Liquid Wastes From Bathrooms, Kitchens Etc.) are discarded as useless or un

Explain the components of heart sounds, Explain the Components of heart sou...

Explain the Components of heart sounds? The first major component is associated with mitral valve closure (M 1) and is due to abrupt arrest of the leaflet motion when the cusps

Explain food lipids, Explain Food lipids It is either consumed in the ...

Explain Food lipids It is either consumed in the form of "visible" fats, which have been separated from the original plant or animal sources, such as vegetable oil and butter,

Solution of ampicillin, How much ampicillin (sodium sal, mw=371.40) would y...

How much ampicillin (sodium sal, mw=371.40) would you dissolve in 400 mL of water to make 80 mg/ml solution of ampicillin?

Do fats with one or more ''kinky'' tail fatty acids, Do fats with one or mo...

Do fats with one or more 'kinky' tail fatty acids tend to be solid or liquid at room temperature? These are found in triglycerides forming what? Solid fats or oils? Is it opposite

In which phase of the cell cycle does the dna duplicate, Q How are the conc...

Q How are the concepts of chromosome, chromatids and chromatin related? In which phase of the cell cycle does the DNA duplicate? Chromatin is a set of filamentous the DNA molec

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd