Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define the Principle behind Cyanmethemoglobin Method?
The principle behind the cyanmethemoglobin method is as follow:
Principle The haemoglobin is treated with a reagent containing potassium cyanide and sodium carbonate. With haemoglobin ferricyanide forms methaemoglobin which is converted to cyanmethaemoglobin or haemiglobincyanide (HiCN) with cyanide. The absorbance is measured at 540 nm. The reaction is presented herewith:
Haemoglobin + potassium ferricyanide ------→ Methaemoglobin
Methaemoglobin + potassium cyanide ------→ Cyanmethaemoglobin (HiCN)
in solution the ferrous ions of the haemoglobin are oxidized to the ferric state by potassium fericyanide to form methaemoglobin. In turn methaemoglobin reacts with cyanide ions (CN-) provided by potassium cyanide to form HiCN. The time required for full colour development is shortened by if dihydrogen potassium phosphate is substituted for sodium bicarbonate in the classic Drabkin's reagent. The colour is stable for more than 24 hours in well stoppered tubes kept in dark. An HiCN standard can be used to calibrate the spectrophotometer, The concentration of HiCN in the sample is calculated from the absorbance in a calibrated spectrophotometer or alternatively with the help of a standard curve.
LIGHT All of us know that the sun is the ultimate source of energy for all activities in our biosphere. The electromagnetic radiations from the sun supply energy which warms up t
Q. What is the difference between spermatocyte I and spermatogonium? The male germ cells are the spermatogonia (diploid cells, 2n) situated in the testicles and they mature an
What is the most accepted hypothesis about the origin of life on earth? How does it compare to the other main hypotheses? The heterotrophic hypothesis is the strongest and most
Define the Sun and Solar Drying Method? The make use of solar energy to preserve the food items is mainly the oldest form of food reservation and is still practiced today for f
Explain colloidal state Foods contain a high percentage of water in which other nutrients present are dispersed. The existence of the colloidal state was first recognized by T
Q. What happens when the oxygen supply is inadequate to maintain aerobic cellular respiration during muscle exercise? If oxygen from myoglobin or hemoglobin is not enough for t
What is the photoperiod? The Photoperiod is the daily time period of light exposure of a living being and the photoperiod may differ according to the period of the year.
Meiosis becomes significant for the following reasons. Constant Number of Chromosomes: It brings about a reduction in the chromosome number from a diploid (2n) condition to
Permeability Permeability is the ability of a soil to transmit water or air. Permeability or infiltration rate is measured in terms of the rate of water flow through the soil i
Role of Wound Epidermis and Apical Epidermal Cap Earlier described that amputation, the stump epidermal cells at the wound edge migrate over and quickly cover the wound to fro
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd