Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define the Principle behind Cyanmethemoglobin Method?
The principle behind the cyanmethemoglobin method is as follow:
Principle The haemoglobin is treated with a reagent containing potassium cyanide and sodium carbonate. With haemoglobin ferricyanide forms methaemoglobin which is converted to cyanmethaemoglobin or haemiglobincyanide (HiCN) with cyanide. The absorbance is measured at 540 nm. The reaction is presented herewith:
Haemoglobin + potassium ferricyanide ------→ Methaemoglobin
Methaemoglobin + potassium cyanide ------→ Cyanmethaemoglobin (HiCN)
in solution the ferrous ions of the haemoglobin are oxidized to the ferric state by potassium fericyanide to form methaemoglobin. In turn methaemoglobin reacts with cyanide ions (CN-) provided by potassium cyanide to form HiCN. The time required for full colour development is shortened by if dihydrogen potassium phosphate is substituted for sodium bicarbonate in the classic Drabkin's reagent. The colour is stable for more than 24 hours in well stoppered tubes kept in dark. An HiCN standard can be used to calibrate the spectrophotometer, The concentration of HiCN in the sample is calculated from the absorbance in a calibrated spectrophotometer or alternatively with the help of a standard curve.
Define Fraction in which Calcium in plasma is Present? Calcium in plasma is present in three major fractions: Ionized calcium Protein bound calcium Complexed c
Spermiogenesis - Spermatogenesis At the end of the meiosis the spermatids appear as simple spherical cells with a centrally located nucleus. Their differentiation into sperm r
Q. Which kind of polarity do fat-soluble and water-soluble substances respectively have? Ans. Water-soluble substances are behave as polar molecules, i.e., they have e
Define Overall positive health Effect of Carbohydrate? Non-glyceinic carbohydrates including non-starch polysaccharides are beneficial for the functions and physiology of gastr
Q. Define cerebral hemisphere tissue? CEREBRAL WHITE MATTER - The remaining cerebral hemisphere tissue is the deeper cerebral white matter (60% of cerebral volume) which includ
Explain about Stacking faults Stacking faults happen in a number of crystal structures, but the common example is in close-packed structures. Face-centered cubic (fcc) struct
mechanisms of reciprocal inhibition
What are the inheritance and dominance patterns of the Rh blood system? An inheritance pattern of the Rh blood system is autosomal dominant that is the heterozygous manifests a
Define Paediatric Problems and Nutritional Management? The process of accepting and digesting food in adequate amounts to meet nutrition needs is termed as feeding. Certain gro
What are plankton, nekton and benthos? Plankton, nekton and benthos are the three groups into which aquatic living beings may be separated. The plankton is formed by the alg
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd