Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define the Principle behind Cyanmethemoglobin Method?
The principle behind the cyanmethemoglobin method is as follow:
Principle The haemoglobin is treated with a reagent containing potassium cyanide and sodium carbonate. With haemoglobin ferricyanide forms methaemoglobin which is converted to cyanmethaemoglobin or haemiglobincyanide (HiCN) with cyanide. The absorbance is measured at 540 nm. The reaction is presented herewith:
Haemoglobin + potassium ferricyanide ------→ Methaemoglobin
Methaemoglobin + potassium cyanide ------→ Cyanmethaemoglobin (HiCN)
in solution the ferrous ions of the haemoglobin are oxidized to the ferric state by potassium fericyanide to form methaemoglobin. In turn methaemoglobin reacts with cyanide ions (CN-) provided by potassium cyanide to form HiCN. The time required for full colour development is shortened by if dihydrogen potassium phosphate is substituted for sodium bicarbonate in the classic Drabkin's reagent. The colour is stable for more than 24 hours in well stoppered tubes kept in dark. An HiCN standard can be used to calibrate the spectrophotometer, The concentration of HiCN in the sample is calculated from the absorbance in a calibrated spectrophotometer or alternatively with the help of a standard curve.
Define Advantages and Disadvantages of Measurement of Cell Mass? Advantage 1. It is a useful technique for estimating fungal and actinomycetes growth. Disadvantages 1.
Q. What are the consequences of shifting the chemical equilibrium of the formation of bicarbonate from water and carbon dioxide towards the consumption of products of the reverse r
Q. What are the major events of the final mitotic period? The final mitotic phase is the telophase. In the telophase the following events occur decondensation of chromosomes ea
Q. What is Primary Motor Cortex? Located in precentral gyrus of frontal lobe (anterior to central sulcus, which separates frontal and parietal lobes. Neurons called pyramidal
Determine about the scales developed by golden Several other scales have been developed by Golden and various collaborators, all of which are based on different ways of scoring
Shape and size of protiens Fibrous proteins for example: keratin in hair, actin and myosin in muscles, and collagen. Globular proteins, for example enzymes and antibo
Over the past decade, the conceptual understanding of heart failure has changed significantly. Several large clinical trials have demonstrated that non-pharmacological and pharmaco
What are gibberellins? Where are they produced? Gibberellins are plant hormones that stimulate plant growth, flowering and fruit formation (also parthenocarpy) and the germina
HMP shunt in erythrocytes HMP shunt in erythrocytes is of importance due to the generation of NADPH, which maintains the glutathione (G-SH) in the reduced state by glutathi
a soil surface of 10cm2 may contain what type of hyphae?
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd