Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define the Principle behind Cyanmethemoglobin Method?
The principle behind the cyanmethemoglobin method is as follow:
Principle The haemoglobin is treated with a reagent containing potassium cyanide and sodium carbonate. With haemoglobin ferricyanide forms methaemoglobin which is converted to cyanmethaemoglobin or haemiglobincyanide (HiCN) with cyanide. The absorbance is measured at 540 nm. The reaction is presented herewith:
Haemoglobin + potassium ferricyanide ------→ Methaemoglobin
Methaemoglobin + potassium cyanide ------→ Cyanmethaemoglobin (HiCN)
in solution the ferrous ions of the haemoglobin are oxidized to the ferric state by potassium fericyanide to form methaemoglobin. In turn methaemoglobin reacts with cyanide ions (CN-) provided by potassium cyanide to form HiCN. The time required for full colour development is shortened by if dihydrogen potassium phosphate is substituted for sodium bicarbonate in the classic Drabkin's reagent. The colour is stable for more than 24 hours in well stoppered tubes kept in dark. An HiCN standard can be used to calibrate the spectrophotometer, The concentration of HiCN in the sample is calculated from the absorbance in a calibrated spectrophotometer or alternatively with the help of a standard curve.
Q. What are the major representatives of the pteridophytes? Is this plant group phanerogamic or cryptogamic? The better known pteridophytes are the ferns and the maidenhairs, f
SOIL TEXTURE AND STRUCTURE We have seen that soils are predominantly composed of particles of various sizes derived from the disintegration or decomposition of parent rock materi
EXPLAIN THE CONSTITUENT OF FOOD GROUPS AND THEIR SOURCES
The Accident or Incident Theory - Accident Causation Peterson when reiterating the significance of human factors added such factors as ergonomic traps, the decision to err and
Describe the Capillarity Theory in respect of ascent of water in plants. Name the tissue included. Determine the initiation of muscle contraction. What is the role of Sarcoplas
Explain the term-Diffusion Diffusion occurs from the side of greater concentration to the side of lesser concentration. In aqueous humour this occurs from the plasma side in th
Regression of Primitive Streak As the inward migration of cells by the primitive streak ends progressively along its anterior to posterior regions the streak slowly regre
What is the importance of vitamin A The importance of vitamin A is undisputable. You may already be aware about the functions/role of vitamin A in our body. Vitamin A is absol
Adsk question #Minimum 100 words accepted#saliva enzyme
Are sperms being attacked by the body since they are foreign?
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd