Define the principle behind cyanmethemoglobin method, Biology

Assignment Help:

Define the Principle behind Cyanmethemoglobin Method?

The principle behind the cyanmethemoglobin method is as follow:

Principle The haemoglobin is treated with a reagent containing potassium cyanide and sodium carbonate. With haemoglobin ferricyanide forms methaemoglobin which is converted to cyanmethaemoglobin or haemiglobincyanide (HiCN) with cyanide. The absorbance is measured at 540 nm. The reaction is presented herewith:

Haemoglobin + potassium ferricyanide ------→ Methaemoglobin

Methaemoglobin + potassium cyanide ------→ Cyanmethaemoglobin (HiCN)

in solution the ferrous ions of the haemoglobin are oxidized to the ferric state by potassium fericyanide to form methaemoglobin. In turn methaemoglobin reacts with cyanide ions (CN-) provided by potassium cyanide to form HiCN. The time required for full colour development is shortened by if dihydrogen potassium phosphate is substituted for sodium bicarbonate in the classic Drabkin's reagent. The colour is stable for more than 24 hours in well stoppered tubes kept in dark. An HiCN standard can be used to calibrate the spectrophotometer, The concentration of HiCN in the sample is calculated from the absorbance in a calibrated spectrophotometer or alternatively with the help of a standard curve.


Related Discussions:- Define the principle behind cyanmethemoglobin method

Show major complications of hypertension, Q. Show Major complications of hy...

Q. Show Major complications of hypertension? High blood pressure is one of the leading causes of kidney failure, also commonly called end-stage renal disease (ESRD).  Major

Requirements and recommended dietary allowances (rda), Define Requirements ...

Define Requirements and Recommended Dietary Allowances (RDA)? What do we mean by requirements and RDA? The requirement level is the amount of nutrient needed to be absorbed to

Mitosis, What is the role of mitosis in growth?

What is the role of mitosis in growth?

POPULATION, WHAT ARE THE CHARACTERISTICS OF POPULATION IN ANIMAL

WHAT ARE THE CHARACTERISTICS OF POPULATION IN ANIMAL

Procedure of hormone act, Procedure of Hormone Act All plant hormones ...

Procedure of Hormone Act All plant hormones show extraordinary varied complex effects in controlling plant growth and development, Extrapolation from how an animal hormone wor

Names and uses of the various laboratory tools, What are the names and uses...

What are the names and uses of the various laboratory tools? Tools are beakers, microscopes, tweezers, hot plates, lasers, test tubes, voltmeters, Erlenmeyer flasks, thermomete

What is the difference between taeniasis and cysticercosis, What is the dif...

What is the difference between taeniasis and cysticercosis? Taeniasis is the parasitic disease caused by the adult tapeworm installed within the human intestine. Cysticercos

Explain alterations occurring in milk and milk products, Alterations occurr...

Alterations occurring in milk and milk products In the dairy industry, milk is commonly given heat treatment for a wide variety of purposes. Depending on the heating temperatur

What is the function of a control group in an experiment, What is the funct...

What is the function of a control group in an experiment? The function of a control group in an experiment is to be a stable to account for any effects a given environment has

Physical map, Physical map is the map of locations of the identifiable lan...

Physical map is the map of locations of the identifiable landmarks on DNA (for example restriction of the enzyme cutting sites, genes), regardless of their inheritance. Distance i

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd