Define the principle behind cyanmethemoglobin method, Biology

Assignment Help:

Define the Principle behind Cyanmethemoglobin Method?

The principle behind the cyanmethemoglobin method is as follow:

Principle The haemoglobin is treated with a reagent containing potassium cyanide and sodium carbonate. With haemoglobin ferricyanide forms methaemoglobin which is converted to cyanmethaemoglobin or haemiglobincyanide (HiCN) with cyanide. The absorbance is measured at 540 nm. The reaction is presented herewith:

Haemoglobin + potassium ferricyanide ------→ Methaemoglobin

Methaemoglobin + potassium cyanide ------→ Cyanmethaemoglobin (HiCN)

in solution the ferrous ions of the haemoglobin are oxidized to the ferric state by potassium fericyanide to form methaemoglobin. In turn methaemoglobin reacts with cyanide ions (CN-) provided by potassium cyanide to form HiCN. The time required for full colour development is shortened by if dihydrogen potassium phosphate is substituted for sodium bicarbonate in the classic Drabkin's reagent. The colour is stable for more than 24 hours in well stoppered tubes kept in dark. An HiCN standard can be used to calibrate the spectrophotometer, The concentration of HiCN in the sample is calculated from the absorbance in a calibrated spectrophotometer or alternatively with the help of a standard curve.


Related Discussions:- Define the principle behind cyanmethemoglobin method

Which structures of the body have more bones, Q. Which structures of the bo...

Q. Which structures of the body have more bones for their size than any other part of the body? Hand and wrist have more bones in them for their size than any other part of bod

Segmentation - metazoa, Segmentation - Metazoa Segmentation or metamer...

Segmentation - Metazoa Segmentation or metamerism is the division of the body into smaller transverse compartments along the anterior-posterior axis. Segmentation is widesprea

Expression of variability, In the previous sub-section we discussed the pos...

In the previous sub-section we discussed the possible ways by which variabiliti can, be generated. We shall now examine one instance that illustrates the consequence of variability

How low birth weight cause protein energy malnutrition, How Low Birth Weigh...

How Low Birth Weight cause Protein Energy Malnutrition? The beginning of PEM in children starts in US from the time of their birth. At least a third of the Indian children are

Explain about cold preservation - methods of food processing, Explain about...

Explain about Cold Preservation - methods of food processing? The metabolism of a living tissue is a function of the temperature of the environment. Low temperatures are used t

Why bacteria called extremophiles, Bacteria called extremophiles are able t...

Bacteria called extremophiles are able to grow in hot springs such as Old Faithful at Yellowstone National Park in Wyoming. Do you think that the DNA of extremophiles would have a

Baby delivery, some deliveries of baby occur early na. y is it so?

some deliveries of baby occur early na. y is it so?

Zoonoses disease-listeriosis, Listeriosis Listeriosis is an important f...

Listeriosis Listeriosis is an important food-borne zoonosis caused by pathogenic species of Listeria. The disease affects both in man and animals. It is a serious invasive dise

What is the parasite that causes toxoplasmosis, Q. What is the parasite tha...

Q. What is the parasite that causes toxoplasmosis? How is the disease transmitted and what are its typical manifestations? The Toxoplasmosis is caused by the protozoan Toxoplas

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd