Define the principle behind cyanmethemoglobin method, Biology

Assignment Help:

Define the Principle behind Cyanmethemoglobin Method?

The principle behind the cyanmethemoglobin method is as follow:

Principle The haemoglobin is treated with a reagent containing potassium cyanide and sodium carbonate. With haemoglobin ferricyanide forms methaemoglobin which is converted to cyanmethaemoglobin or haemiglobincyanide (HiCN) with cyanide. The absorbance is measured at 540 nm. The reaction is presented herewith:

Haemoglobin + potassium ferricyanide ------→ Methaemoglobin

Methaemoglobin + potassium cyanide ------→ Cyanmethaemoglobin (HiCN)

in solution the ferrous ions of the haemoglobin are oxidized to the ferric state by potassium fericyanide to form methaemoglobin. In turn methaemoglobin reacts with cyanide ions (CN-) provided by potassium cyanide to form HiCN. The time required for full colour development is shortened by if dihydrogen potassium phosphate is substituted for sodium bicarbonate in the classic Drabkin's reagent. The colour is stable for more than 24 hours in well stoppered tubes kept in dark. An HiCN standard can be used to calibrate the spectrophotometer, The concentration of HiCN in the sample is calculated from the absorbance in a calibrated spectrophotometer or alternatively with the help of a standard curve.


Related Discussions:- Define the principle behind cyanmethemoglobin method

Updating by deletion, Updating by Deletion DELETE FROM ENROLMENT WHERE...

Updating by Deletion DELETE FROM ENROLMENT WHERE StudentId = SID ('S4'); As you can see, this differs from Example by the addition of the noise word FROM. The expression SI

Explain adverse effects of ribavirin, Adverse Effects of Ribavirin  Sys...

Adverse Effects of Ribavirin  Systemic ribavirin has been associated with hemolytic anemia. Oral ribavirin plus interferon appears to cause a higher incidence of cough, pruritu

Theoretical calculation of calorific value of a fuel, The calorific value o...

The calorific value of a fuel can be theoretically determined by using dulong's formula. It is assumed that heat evolved comes from the combustion of carbon, hydrogen, and sulphur

How does the excretory system of nematodes work, Q How does the excretory s...

Q How does the excretory system of nematodes work? The metabolic residuals of nematodes are together by two longitudinal lateral excretory channels that open in one single excr

Duration limit of the qrs, Q. Duration limit of the QRS? The duration o...

Q. Duration limit of the QRS? The duration of the QRS is usually reduced slightly during exercise. Because catecholamines increase conduction velocity in the Purkinje fibers an

Requirements and recommended dietary allowances (rda), Define Requirements ...

Define Requirements and Recommended Dietary Allowances (RDA)? What do we mean by requirements and RDA? The requirement level is the amount of nutrient needed to be absorbed to

What the henderson-hasselbalch equation expresses, The Henderson-Hasselbalc...

The Henderson-Hasselbalch equation expresses the pH of a solution as: Select one: a. The equilibrium constant of the acid and the dissociated form of the weak acid. b. The

Describe the features common in ventricular outflow, Describe the Features ...

Describe the Features common in ventricular outflow obstruction ? An ejection systolic murmur (ESM) due turbulent flow of blood through the obstruction. Hypertrophy of the cham

What is constipation, Q. What is Constipation? Constipation is irregula...

Q. What is Constipation? Constipation is irregular, infrequent or difficult passage of faeces. It is the most common physiological disorder of the alimentary tract. It is chara

Taxonomy, what arachnid is beneficial

what arachnid is beneficial

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd