Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define the Principle behind Cyanmethemoglobin Method?
The principle behind the cyanmethemoglobin method is as follow:
Principle The haemoglobin is treated with a reagent containing potassium cyanide and sodium carbonate. With haemoglobin ferricyanide forms methaemoglobin which is converted to cyanmethaemoglobin or haemiglobincyanide (HiCN) with cyanide. The absorbance is measured at 540 nm. The reaction is presented herewith:
Haemoglobin + potassium ferricyanide ------→ Methaemoglobin
Methaemoglobin + potassium cyanide ------→ Cyanmethaemoglobin (HiCN)
in solution the ferrous ions of the haemoglobin are oxidized to the ferric state by potassium fericyanide to form methaemoglobin. In turn methaemoglobin reacts with cyanide ions (CN-) provided by potassium cyanide to form HiCN. The time required for full colour development is shortened by if dihydrogen potassium phosphate is substituted for sodium bicarbonate in the classic Drabkin's reagent. The colour is stable for more than 24 hours in well stoppered tubes kept in dark. An HiCN standard can be used to calibrate the spectrophotometer, The concentration of HiCN in the sample is calculated from the absorbance in a calibrated spectrophotometer or alternatively with the help of a standard curve.
In what way may we be able to take advantage of retrotransposons in human gene therapy? How would this differ from our current use of retroviruses?
Q. Is there a difference between the initial and the final energy levels in non-catalyzed and catalyzed reactions? The catalysis does not modify the energetic state of reagents
Lauric Fats Fats of this group are derived from certain species of palm, such as coconut. The fats are characterized by their high content of lauric acid (40 - 50%), moderate
Q. How do bones grow? Bones form and develop through a process known as ossification. There are two types of ossification: endochondral ossification and intramembranous ossific
what websites can i use to modify my client''s meals?
Healthy human female X is 25 years old and not pregnant. During the postovulatory phase of X's menstrual cycle, A. there are high blood plasma levels of LH and FSH.
what is skeleton?
Defence mechanisms of the host The healthy animal is protected from pathogenic organisms present in the environment by virtue of the effective immune mechanism present from bi
Collagen Helix Collagen has a large amount of glycine (25%) and proline (and hydroxyproline, 25%). They run parallel, form a right handed super-helix that is stabilized b
Radioisotopes Radioisotopes are those forms of an element which exhibit rzdioactivity and so can be used as markers or tracers. For this purpose, we first introduce the radiois
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd