Define the principle behind cyanmethemoglobin method, Biology

Assignment Help:

Define the Principle behind Cyanmethemoglobin Method?

The principle behind the cyanmethemoglobin method is as follow:

Principle The haemoglobin is treated with a reagent containing potassium cyanide and sodium carbonate. With haemoglobin ferricyanide forms methaemoglobin which is converted to cyanmethaemoglobin or haemiglobincyanide (HiCN) with cyanide. The absorbance is measured at 540 nm. The reaction is presented herewith:

Haemoglobin + potassium ferricyanide ------→ Methaemoglobin

Methaemoglobin + potassium cyanide ------→ Cyanmethaemoglobin (HiCN)

in solution the ferrous ions of the haemoglobin are oxidized to the ferric state by potassium fericyanide to form methaemoglobin. In turn methaemoglobin reacts with cyanide ions (CN-) provided by potassium cyanide to form HiCN. The time required for full colour development is shortened by if dihydrogen potassium phosphate is substituted for sodium bicarbonate in the classic Drabkin's reagent. The colour is stable for more than 24 hours in well stoppered tubes kept in dark. An HiCN standard can be used to calibrate the spectrophotometer, The concentration of HiCN in the sample is calculated from the absorbance in a calibrated spectrophotometer or alternatively with the help of a standard curve.


Related Discussions:- Define the principle behind cyanmethemoglobin method

Hypodermic-subcutaneous injection , Hypodermic-Subcutaneous Injection ...

Hypodermic-Subcutaneous Injection By this route the drug is mainly absorbed into the blood stream by way of the lymphatic drainage. Absorption is slower by this route

Why water is considered a universal solvent, Water is considered a universa...

Water is considered a universal solvent because- Select one: a. it can dissolve polar and non-polar compounds b. it can dissolve both positively and negatively charged ions a

Why do leaves change color, Why do leaves change color? Leave is the fo...

Why do leaves change color? Leave is the food factories of plants. During the spring and summer, leaves are actively making food and they are filled with chlorophyll, which giv

Differentiate blood retinal barrier and aqueous barrier, What are the diffe...

What are the differences between blood retinal barrier and blood aqueous barrier? Blood Retinal Barrier: Blood retinal barrier is present in retina. In retina there are t

What are the main human diseases caused by prions, Q. What are the main hum...

Q. What are the main human diseases caused by prions? The major known human diseases of such type are the Creutzfeldt-Jacob disease (CJD), the kuru and the Gerstmann-Sträussle-

What is cellular regeneration, What is cellular regeneration? How is mitosi...

What is cellular regeneration? How is mitosis related to this process? Some tissues are able to regenerate when injured. The liver, for instance, regenerates when small pieces

Determine the list of sensory organs, Determine the list of Sensory Organs ...

Determine the list of Sensory Organs There are four special sensory organs in our body. They are: 1) Eyes 2) Ears 3) Nose and 4) Skin

Determine the cardio-vascular system, Determine the Cardio-vascular System ...

Determine the Cardio-vascular System Cardio-vascular system consists of a heart and blood vessels. The function of cardio- vascular system is to maintain blood supply to all t

State in brief about the neuropsychological tests, State in brief about the...

State in brief about the Neuropsychological tests Neuropsychological tests have to be constructed using the psychometric approach the interpretation of test performance would n

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd