Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define the Principle behind Cyanmethemoglobin Method?
The principle behind the cyanmethemoglobin method is as follow:
Principle The haemoglobin is treated with a reagent containing potassium cyanide and sodium carbonate. With haemoglobin ferricyanide forms methaemoglobin which is converted to cyanmethaemoglobin or haemiglobincyanide (HiCN) with cyanide. The absorbance is measured at 540 nm. The reaction is presented herewith:
Haemoglobin + potassium ferricyanide ------→ Methaemoglobin
Methaemoglobin + potassium cyanide ------→ Cyanmethaemoglobin (HiCN)
in solution the ferrous ions of the haemoglobin are oxidized to the ferric state by potassium fericyanide to form methaemoglobin. In turn methaemoglobin reacts with cyanide ions (CN-) provided by potassium cyanide to form HiCN. The time required for full colour development is shortened by if dihydrogen potassium phosphate is substituted for sodium bicarbonate in the classic Drabkin's reagent. The colour is stable for more than 24 hours in well stoppered tubes kept in dark. An HiCN standard can be used to calibrate the spectrophotometer, The concentration of HiCN in the sample is calculated from the absorbance in a calibrated spectrophotometer or alternatively with the help of a standard curve.
Agroup of realated genera are classified as-?
Q. After the morula stage what is the next stage? What is the morphological feature that defines this stage? In the blastula stage the compactness is lost and an internal cavit
Permeability Permeability is the ability of a soil to transmit water or air. Permeability or infiltration rate is measured in terms of the rate of water flow through the soil i
MUSCLES - In eye orbit, eye ball is fixed by 6 skeletal muscles attached to 3rd, 4th & 6th cranial nerves (motor). 4 - straight or recti muscles are present. 2 - oblique
Illustrate in detail about the Cell theory All living organisms are composed of cells, and they are the basic structural and functional units of life, just like the atom is a
What is the physiological cause of the syndrome known as cretinism? Cretinism is caused by chronic deficiency of the thyroid hormones (T3 and T4) during childhood. The chronic
define the pattern of cleavage i all aspects
Structure and Content of Halsted Reitan battery Although there are several versions of the Halsted Reitan battery, the differences tend to be minor, and there appears to be a
Question 1 How would you perform ABO blood grouping? Add a note on advantages and disadvantages of each method. Also discuss the precautions you may take to avoid errors in variou
Conservative Substitution: The nucleotide mutation that alters the amino acid sequence of protein, but which causes substitution of one amino acid with another which has a side ch
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd