Define the principle behind cyanmethemoglobin method, Biology

Assignment Help:

Define the Principle behind Cyanmethemoglobin Method?

The principle behind the cyanmethemoglobin method is as follow:

Principle The haemoglobin is treated with a reagent containing potassium cyanide and sodium carbonate. With haemoglobin ferricyanide forms methaemoglobin which is converted to cyanmethaemoglobin or haemiglobincyanide (HiCN) with cyanide. The absorbance is measured at 540 nm. The reaction is presented herewith:

Haemoglobin + potassium ferricyanide ------→ Methaemoglobin

Methaemoglobin + potassium cyanide ------→ Cyanmethaemoglobin (HiCN)

in solution the ferrous ions of the haemoglobin are oxidized to the ferric state by potassium fericyanide to form methaemoglobin. In turn methaemoglobin reacts with cyanide ions (CN-) provided by potassium cyanide to form HiCN. The time required for full colour development is shortened by if dihydrogen potassium phosphate is substituted for sodium bicarbonate in the classic Drabkin's reagent. The colour is stable for more than 24 hours in well stoppered tubes kept in dark. An HiCN standard can be used to calibrate the spectrophotometer, The concentration of HiCN in the sample is calculated from the absorbance in a calibrated spectrophotometer or alternatively with the help of a standard curve.


Related Discussions:- Define the principle behind cyanmethemoglobin method

Define nutritional requirement in cold and polar environment, Define Nutrit...

Define Nutritional Requirements in Cold and Polar Environment? Energy requirements are the major consideration for providing nutritional support in a cold environment. Energy e

Define antioxidant action of polyphenols, Define Antioxidant Action of Poly...

Define Antioxidant Action of Polyphenols? The properties of Polyphenols due to which they have been identified to act as antioxidant is because they: - act as termi

Egestion of food, EGESTION To give out undigested food from alimenta...

EGESTION To give out undigested food from alimentary canal. Faeces can remain in colon for 36 hrs. then moving into rectum by gastro-colic reflex. Faeces consists of 3

Where can these epithelia are found in the human body, How dissimilar is th...

How dissimilar is the simple cuboidal epithelium from the columnar epithelium? Where can these epithelia are found in the human body? The simple cuboidal epithelium is made of

Explain the procedure for preparation of bacterial smear, Explain the Proce...

Explain the Procedure for Preparation of Bacterial Smear? Start the exercise by following the steps enumerated herewith. (1) Take properly washed and dried glass slides for

What is the average duration of each stage in hours, A biologist examines a...

A biologist examines a series of cells and counts 140 cells in interphase, 10 cells in metaphase, 4 cells in anaphase and 7 cells in telophase. if complete cell cycle requires 24 h

Virus is in an inactive state, Q. What is signifying when it is said that a...

Q. What is signifying when it is said that a virus is in an inactive state? Viruses considered in inactive state are those whose genetic material is within host cells without a

Menstrual cycle, MENSTRUAL CYCLE - It occurs only in Primates. Start at...

MENSTRUAL CYCLE - It occurs only in Primates. Start at the age of 13 yrs. upto menopouse. During this period, generally one egg is released every 28 dyas or so alternately f

What is fontan operation and modifications, What is Fontan Operation and Mo...

What is Fontan Operation and Modifications ? Earlier reported to have 20 per cent mortality, it has come down to five per cent in specialized centres. In the earlier series th

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd