Define the principle behind cyanmethemoglobin method, Biology

Assignment Help:

Define the Principle behind Cyanmethemoglobin Method?

The principle behind the cyanmethemoglobin method is as follow:

Principle The haemoglobin is treated with a reagent containing potassium cyanide and sodium carbonate. With haemoglobin ferricyanide forms methaemoglobin which is converted to cyanmethaemoglobin or haemiglobincyanide (HiCN) with cyanide. The absorbance is measured at 540 nm. The reaction is presented herewith:

Haemoglobin + potassium ferricyanide ------→ Methaemoglobin

Methaemoglobin + potassium cyanide ------→ Cyanmethaemoglobin (HiCN)

in solution the ferrous ions of the haemoglobin are oxidized to the ferric state by potassium fericyanide to form methaemoglobin. In turn methaemoglobin reacts with cyanide ions (CN-) provided by potassium cyanide to form HiCN. The time required for full colour development is shortened by if dihydrogen potassium phosphate is substituted for sodium bicarbonate in the classic Drabkin's reagent. The colour is stable for more than 24 hours in well stoppered tubes kept in dark. An HiCN standard can be used to calibrate the spectrophotometer, The concentration of HiCN in the sample is calculated from the absorbance in a calibrated spectrophotometer or alternatively with the help of a standard curve.


Related Discussions:- Define the principle behind cyanmethemoglobin method

Discuss about the halstead reitan battery, Discuss about the Halstead Reita...

Discuss about the Halstead Reitan battery The Halstead Reitan battery continues to be widely used as a clinical and research procedure. Numerous investigators use it in their r

Which type of cell came first in evolution, Which type of cell came first i...

Which type of cell came first in evolution - the eukaryotic cell or the prokaryotic cell? This is an interesting problem of biological evolution. The most accepted hypothesis a

Cleft palate, Cleft Palate   Well you have now learnt about the cleft l...

Cleft Palate   Well you have now learnt about the cleft lip and now let us discuss and understand what is cleft palate. Cleft palate (CP) is midline fissure of  the palate that

Explain about types of insulin, Q. Explain about types of Insulin? Thre...

Q. Explain about types of Insulin? Three types of Insulin is available. The type varies in how quickly it starts working (lowering blood glucose), time of peak activity (when t

What is structural formula of glycerol, Q. What is structural formula of gl...

Q. What is structural formula of glycerol? To which organic function do these molecules belong? Glycerol is a linear chain of three carbons, the central carbon is bound to one

What is the difference between taeniasis and cysticercosis, What is the dif...

What is the difference between taeniasis and cysticercosis? Taeniasis is the parasitic disease caused by the adult tapeworm installed within the human intestine. Cysticercos

Define counter stain - staining technique, Define Counter Stain - Staining ...

Define Counter Stain - Staining Technique? Finally, the smear is counter stained with a simple basic dye different in colour from crystal violet. Safranin is the most commonly

Explain about the derived proteins, Explain about the Derived Proteins? ...

Explain about the Derived Proteins? These are not naturally taking place proteins and are obtained from simple proteins by the action of enzymes and chemical agents, heat, mech

Can you explain listeriosis, Q. Can you explain Listeriosis? Listeriosi...

Q. Can you explain Listeriosis? Listeriosis is a food borne illness caused by a pathogenic bacterium called Listeria monocytogenes, which is food borne. Listeria monocytogenes

What is the excitation threshold of a neuron, Q. What is the excitation thr...

Q. What is the excitation threshold of a neuron? How does this threshold relate to the "all-or-nothing" rule of the neural transmission? The excitation threshold of a neuron is

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd