Define the principle behind cyanmethemoglobin method, Biology

Assignment Help:

Define the Principle behind Cyanmethemoglobin Method?

The principle behind the cyanmethemoglobin method is as follow:

Principle The haemoglobin is treated with a reagent containing potassium cyanide and sodium carbonate. With haemoglobin ferricyanide forms methaemoglobin which is converted to cyanmethaemoglobin or haemiglobincyanide (HiCN) with cyanide. The absorbance is measured at 540 nm. The reaction is presented herewith:

Haemoglobin + potassium ferricyanide ------→ Methaemoglobin

Methaemoglobin + potassium cyanide ------→ Cyanmethaemoglobin (HiCN)

in solution the ferrous ions of the haemoglobin are oxidized to the ferric state by potassium fericyanide to form methaemoglobin. In turn methaemoglobin reacts with cyanide ions (CN-) provided by potassium cyanide to form HiCN. The time required for full colour development is shortened by if dihydrogen potassium phosphate is substituted for sodium bicarbonate in the classic Drabkin's reagent. The colour is stable for more than 24 hours in well stoppered tubes kept in dark. An HiCN standard can be used to calibrate the spectrophotometer, The concentration of HiCN in the sample is calculated from the absorbance in a calibrated spectrophotometer or alternatively with the help of a standard curve.


Related Discussions:- Define the principle behind cyanmethemoglobin method

Bacteria is an obligate anaerobe, Q. What is signifying when it is said tha...

Q. What is signifying when it is said that a bacteria is an obligate anaerobe? Obligate anaerobes are those living beings that do not survive in the presence of oxygen. For exa

Explain the main benefits of canned foods, Explain the Main Benefits of Can...

Explain the Main Benefits of Canned Foods? The main benefits of canned foods are: (a) They are safe and hygienic and have logically good nutritional value; (b) They are e

In hormonal terms why does menses occur, Q. In hormonal terms why does mens...

Q. In hormonal terms why does menses occur? Menses is the endometrial monthly desquamation that occurs as the progesterone and estrogen levels fall after the regression of the

#title., poor metabolism phenotype will have

poor metabolism phenotype will have

Functions of skin, FUNCTIONS OF SKIN - Skin performs various diverse fu...

FUNCTIONS OF SKIN - Skin performs various diverse functions, that is why it is called "jack of all trades". 1 .      Protection - The skin protects the internal soft or

Retention of soil moisture, Retention of Soil Moisture The movement of ...

Retention of Soil Moisture The movement of water into and within the soil, moisture storing capacity of soils and the availability of moisture to plants are governed by soil pr

Explain various chemical formulas, Explain various chemical formulas? C...

Explain various chemical formulas? Chemical Formulas A molecule can be represented in any of three ways: its chemical formula, structural formula, or Lewis diagram. A chemic

What are his morning and bedtime doses, a) Donald is a 50 year old male wit...

a) Donald is a 50 year old male with a long history of bipolar disorder. He has been hospitalized three times in the last 8 months for manic episodes. He was just admitted to your

When conducting the hair comparison, When conducting a hair comparison, wha...

When conducting a hair comparison, what factors would you consider?

Show metabolic rate of aerobic organisms, Q. Why can be the consumption of ...

Q. Why can be the consumption of molecular oxygen indicates the metabolic rate of aerobic organisms? Molecular oxygen (O 2 ) consumption has direct relation to the cell metabol

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd