Define the primary stain and mordant, Biology

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Define the Primary Stain and Mordant?

(i) Primary Stain - Crystal violet is the primary or first stain, which stains all the cells violet/purple.

(ii) Mordant - Gram's iodine serves as mordant. It binds with primary stain to form insoluble complex, crystal violet-iodine (CV-I) complex, that intensify the colour of the stain. At this point, all cells will appear purple - black.

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