Define the fehling's soxhlet method (lane-eynon method), Biology

Assignment Help:

Define the Fehling's Soxhlet method (Lane-Eynon method)?

This is a titrimetric method that is commonly used in food laboratories to estimate percentage of reducing sugars and total sugars. The method is also applicable to biological fluids like milk. The Lane-Eynon method is a titration method of determining the concentration of reducing sugars in a sample. A burette is employed to add the carbohydrate solution being analyzed to a flask consisting of a fixed amount of boiling copper sulfate solution and a methylene blue indicator.

The decreasing sugars in the carbohydrate solution react along with the copper sulfate present in the flask. Once all the copper sulfate in solution has reacted, any further addition of reducing sugars causes the indicator to change from blue to colourless. The volume of sugar solution needed to reach the end point is recorded. Reducing sugars like glucose, fructose, galactose, lactose and maltose can be estimated by direct titration. Non-reducing carbohydrates like sucrose, dextrins and starches cannot be directly estimated by this method. They have to be hydrolyzed into reducing monosaccharides and then estimated titrimetrically. The Fehling Soxhlet method or lane Eynon method is based on oxidation-reduction reaction. You are already familiar with oxidation-reduction tests used for identification of reducing sugars. The basic principle is that sugars in alkaline medium form enediols which reduce cupric ions to cuprous ions. In turn, sugars are oxidized to a mixture of sugar acids. 


Related Discussions:- Define the fehling's soxhlet method (lane-eynon method)

What is a terrestrial organism, Explain what is a terrestrial organism? ...

Explain what is a terrestrial organism? Ans) 'Terra' is the Latin word for earth. Thus, an animal that lives on the surface of the earth is known as terrestrial. This is the si

Poultry and duck diseases-infectious bursal disease (ibd), Infectious bursa...

Infectious bursal disease (IBD) It is a highly contagious acute disease in young chicken up to 6 weeks of age causing high morbidity and low mortality. Bursa may atrophy at th

Centrifugal pump-type of blood pump, Centrifugal Pump: This is available ...

Centrifugal Pump: This is available for clinical perfusion from 1976 (Bio Medicus Pump). It is disposable, causes less blood trauma and reduces the risk of massive air embolism.

Protozoa, what are disadvantage of protozoa

what are disadvantage of protozoa

Write the blood sugar in normal range and range in diabetes, Q. Write the b...

Q. Write the blood sugar in normal range and range in diabetes? Normal           Diabetes mellitus >126 mg / dl >200 mg / dl

Excretion organ, why excretory organ of prawn is called green gland?

why excretory organ of prawn is called green gland?

Define three major steps for study of genetic family tree, What are the thr...

What are the three major steps for a good study of a genetic family tree? Step 1: Determine whether the studied phenotypical form has a dominant or the recessive pattern. Step

Difference between smooth and rough endoplasmic reticulum, What is the diff...

What is the difference among smooth and rough endoplasmic reticulum?       Ans) The endoplasmic reticulum is a fine membranous structure contiguous to the nuclear membrane and p

Respiration, explain the respiration that takes place in different organism...

explain the respiration that takes place in different organisms??

Explain the metabolic rate of aerobic organisms, Why can the consumption of...

Why can the consumption of molecular oxygen indicate the metabolic rate of aerobic organisms? Molecular oxygen (O2) consumption has direct relation to the cell metabolic rate i

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd