Define the fehling's soxhlet method (lane-eynon method), Biology

Assignment Help:

Define the Fehling's Soxhlet method (Lane-Eynon method)?

This is a titrimetric method that is commonly used in food laboratories to estimate percentage of reducing sugars and total sugars. The method is also applicable to biological fluids like milk. The Lane-Eynon method is a titration method of determining the concentration of reducing sugars in a sample. A burette is employed to add the carbohydrate solution being analyzed to a flask consisting of a fixed amount of boiling copper sulfate solution and a methylene blue indicator.

The decreasing sugars in the carbohydrate solution react along with the copper sulfate present in the flask. Once all the copper sulfate in solution has reacted, any further addition of reducing sugars causes the indicator to change from blue to colourless. The volume of sugar solution needed to reach the end point is recorded. Reducing sugars like glucose, fructose, galactose, lactose and maltose can be estimated by direct titration. Non-reducing carbohydrates like sucrose, dextrins and starches cannot be directly estimated by this method. They have to be hydrolyzed into reducing monosaccharides and then estimated titrimetrically. The Fehling Soxhlet method or lane Eynon method is based on oxidation-reduction reaction. You are already familiar with oxidation-reduction tests used for identification of reducing sugars. The basic principle is that sugars in alkaline medium form enediols which reduce cupric ions to cuprous ions. In turn, sugars are oxidized to a mixture of sugar acids. 


Related Discussions:- Define the fehling's soxhlet method (lane-eynon method)

Define nutritional needs during recovery, Define nutritional needs During R...

Define nutritional needs During Recovery? Here, let us discuss what should be the nutritional goals based on the physiological aspects involved. Goals: The main emphasis mus

Define criteria for assessment of vitamin k status, Define Criteria for Ass...

Define Criteria for Assessment of Vitamin K Status? The parameters of blood clotting time and prothrombin time are used as criteria  to assess vitamin K status as this vitamin

Why iron is required as a part of haemoglobin, Why Iron is required as a pa...

Why Iron is required as a part of haemoglobin? As a part of haemoglobin, iron is required for the transport of oxygen, to all cells in the body. Thus, haemoglobin is critical f

What are deciduous trees, What are deciduous trees? Deciduous trees are...

What are deciduous trees? Deciduous trees are plants that lose their leaves in a period of the year. In the case of the deciduous trees of the temperate forest the fall of the

Are protozoans presenting pulsatile, Q. Are protozoans presenting pulsatile...

Q. Are protozoans presenting pulsatile or contractile vacuoles easily found in fresh or in salt water? Fresh water is the less concentrated of solutes than sea water and it fre

What are the parathyroids, Q. What are the parathyroids? Where are they loc...

Q. What are the parathyroids? Where are they located and what are the hormones secreted by these glands? The parathyroids are four small glands embedded two in each posterior f

Determine the deamination during rna editing, Which of the following is not...

Which of the following is not needed for C to U deamination during RNA editing? A. Formation of localized segments of double stranded RNA. B. The 5' and 3' efficiency elemen

Explain the importance for a hypothesis, What's the difference between theo...

What's the difference between theory and hypothesis and explain the importance for a hypothesis to be testable and falsifiable in order for the scientific method to be applied?

Explain two capacities and volumes of respiratory, Given four respiratory c...

Given four respiratory capacities (a - d) and four jumbled respiratory volumes of a normal human adult: Respiratory Respiratory capacities volumes. 1.            Residual vol

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd