Define the fehling's soxhlet method (lane-eynon method), Biology

Assignment Help:

Define the Fehling's Soxhlet method (Lane-Eynon method)?

This is a titrimetric method that is commonly used in food laboratories to estimate percentage of reducing sugars and total sugars. The method is also applicable to biological fluids like milk. The Lane-Eynon method is a titration method of determining the concentration of reducing sugars in a sample. A burette is employed to add the carbohydrate solution being analyzed to a flask consisting of a fixed amount of boiling copper sulfate solution and a methylene blue indicator.

The decreasing sugars in the carbohydrate solution react along with the copper sulfate present in the flask. Once all the copper sulfate in solution has reacted, any further addition of reducing sugars causes the indicator to change from blue to colourless. The volume of sugar solution needed to reach the end point is recorded. Reducing sugars like glucose, fructose, galactose, lactose and maltose can be estimated by direct titration. Non-reducing carbohydrates like sucrose, dextrins and starches cannot be directly estimated by this method. They have to be hydrolyzed into reducing monosaccharides and then estimated titrimetrically. The Fehling Soxhlet method or lane Eynon method is based on oxidation-reduction reaction. You are already familiar with oxidation-reduction tests used for identification of reducing sugars. The basic principle is that sugars in alkaline medium form enediols which reduce cupric ions to cuprous ions. In turn, sugars are oxidized to a mixture of sugar acids. 


Related Discussions:- Define the fehling's soxhlet method (lane-eynon method)

List out points about the traditional food preservation, List out Points ab...

List out Points about the Traditional Food Preservation? 1. The traditional food preservation methods like drying, pickling, salting, smoking, canning and freezing have been wi

Can you explain toxicants in plant foods, Q. Can you explain Toxicants in P...

Q. Can you explain Toxicants in Plant Foods? Several foods, some of which we consume regularly, have toxic compounds in them which are best avoided. These compounds can be prot

Acetylcholine - long term potentiation, Acetylcholine, a well known neurotr...

Acetylcholine, a well known neurotransmitter, plays a critical synaptic role in the initial formation of memory. Chemical blockage of the acetylcholine receptors makes it harder to

Nucleic acids, Deoxyribonucleic Acid (DNA) - polymer of nucleotides contai...

Deoxyribonucleic Acid (DNA) - polymer of nucleotides containing genetic information that codes for proteins Nucleotide - a monomer of DNA consisting of a ribose/deoxyribose sug

Auxiliary food chains, Auxiliary Food Chains In addition to grazing an...

Auxiliary Food Chains In addition to grazing and detritus food chains there are other auxiliary food chains operated through parasites and scavengers. Some parasitic food chai

Digestive system - small intestine, SMAL L INTESTINE - A narrow tube 6...

SMAL L INTESTINE - A narrow tube 6 m long. In rabbit 4 m. long.Differentiated into duodenum, jejunum & Ileum. (i) Duodenum - 2.5 cm long. C-shaped (U-shaped in rabbit)

How many progeny flies have vestigial wings, In the fruit fly Drosophila, a...

In the fruit fly Drosophila, a rudimentary wing called "vestigial" and dark body color called "ebony" are inherited at independent loci and are recessive to their dominant wild-typ

Essentials of Human Diseases and Conditions, How do pathological conditions...

How do pathological conditions affect homeostasis of your body?

Digestive System, When a piece of thread is tied tightly around an animal p...

When a piece of thread is tied tightly around an animal pancreatic duct the animal experiences difficult in digestion but does not suffer from diabetes mellitus.Explain why?

Explain reversion flavor, Reversion flavor:  The rapid oxidation of br...

Reversion flavor:  The rapid oxidation of branched furan fatty acids on exposure to light, leads to the production of an intensive aromatic substance, which along with diacety

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd