Define the fehling's soxhlet method (lane-eynon method), Biology

Assignment Help:

Define the Fehling's Soxhlet method (Lane-Eynon method)?

This is a titrimetric method that is commonly used in food laboratories to estimate percentage of reducing sugars and total sugars. The method is also applicable to biological fluids like milk. The Lane-Eynon method is a titration method of determining the concentration of reducing sugars in a sample. A burette is employed to add the carbohydrate solution being analyzed to a flask consisting of a fixed amount of boiling copper sulfate solution and a methylene blue indicator.

The decreasing sugars in the carbohydrate solution react along with the copper sulfate present in the flask. Once all the copper sulfate in solution has reacted, any further addition of reducing sugars causes the indicator to change from blue to colourless. The volume of sugar solution needed to reach the end point is recorded. Reducing sugars like glucose, fructose, galactose, lactose and maltose can be estimated by direct titration. Non-reducing carbohydrates like sucrose, dextrins and starches cannot be directly estimated by this method. They have to be hydrolyzed into reducing monosaccharides and then estimated titrimetrically. The Fehling Soxhlet method or lane Eynon method is based on oxidation-reduction reaction. You are already familiar with oxidation-reduction tests used for identification of reducing sugars. The basic principle is that sugars in alkaline medium form enediols which reduce cupric ions to cuprous ions. In turn, sugars are oxidized to a mixture of sugar acids. 


Related Discussions:- Define the fehling's soxhlet method (lane-eynon method)

Study of asian chinese of normal range of waveform indices, A Collective St...

A Collective Study performed on Asian Chinese Normotensive Population to Obtain Healthy Normal Range of some Waveform Indices  Background-  Arterial blood pressure waveforms c

Sonu, my question is what is phyletic lineage

my question is what is phyletic lineage

Animal reproduction, Animal Reproduction - Reproduction is the ability ...

Animal Reproduction - Reproduction is the ability to produce new organism of its own type. It is meant to increase their number. Reproduction includes - (i) Replication o

Contractile vacuoles - excretion, Normal 0 false false fals...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Explain energy requirement in postoperative nutritional care, Explain the E...

Explain the Energy requirement in Postoperative Nutritional Care? Adequate calorie intake is critical for successful outcome of surgery and should be provided in the form of ca

VASCULAR PLANTS., WHAT TRAITS ALLOWED VASCULAR PLANTS TO GROW TALL

WHAT TRAITS ALLOWED VASCULAR PLANTS TO GROW TALL

Define a ''rag doll'' seed tester, A 'rag doll' seed tester Fold a squa...

A 'rag doll' seed tester Fold a square metre of muslin twice in the similar direction. Near one end mark out eight or ten squares about 5 cm by 5cm with a pencil. Number the sq

Name the skin receptors in humans, Which of the below are skin receptors...

Which of the below are skin receptors in humans which are sensitive to heat? Are they: a) End organs of Krause b) Meissner's corpuscles c) End organs of Ruffini d)

What are differentiations of the cell membrane, What are differentiations o...

What are differentiations of the cell membrane? In some types of cells, the cell membrane presents differentiations that are essential for the specific functions of the cells.

Determination of neural ectoderm by induction, Determination of Neural Ecto...

Determination of Neural Ectoderm by Induction Earlier you know that the dorsal mesoderm induces the ectoderm to differentiate into neural tissue. Spemann and his co-workers co

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd