Define the fehling's soxhlet method (lane-eynon method), Biology

Assignment Help:

Define the Fehling's Soxhlet method (Lane-Eynon method)?

This is a titrimetric method that is commonly used in food laboratories to estimate percentage of reducing sugars and total sugars. The method is also applicable to biological fluids like milk. The Lane-Eynon method is a titration method of determining the concentration of reducing sugars in a sample. A burette is employed to add the carbohydrate solution being analyzed to a flask consisting of a fixed amount of boiling copper sulfate solution and a methylene blue indicator.

The decreasing sugars in the carbohydrate solution react along with the copper sulfate present in the flask. Once all the copper sulfate in solution has reacted, any further addition of reducing sugars causes the indicator to change from blue to colourless. The volume of sugar solution needed to reach the end point is recorded. Reducing sugars like glucose, fructose, galactose, lactose and maltose can be estimated by direct titration. Non-reducing carbohydrates like sucrose, dextrins and starches cannot be directly estimated by this method. They have to be hydrolyzed into reducing monosaccharides and then estimated titrimetrically. The Fehling Soxhlet method or lane Eynon method is based on oxidation-reduction reaction. You are already familiar with oxidation-reduction tests used for identification of reducing sugars. The basic principle is that sugars in alkaline medium form enediols which reduce cupric ions to cuprous ions. In turn, sugars are oxidized to a mixture of sugar acids. 


Related Discussions:- Define the fehling's soxhlet method (lane-eynon method)

Explain the klebsiella - characteristics of bacteria, Explain the Klebsiell...

Explain the Klebsiella - Characteristics of Bacteria? It is gram negative, non-sporulating, non-motile and non-pigmented rod, which is commonly found in soil or water. It is th

How will it impact the rest of the organs, Starting at the esophagus, trace...

Starting at the esophagus, trace the pathway of food through the system. At each organ, indicate anatomical adaptations to the general GI tract tube structure that enhance the spec

Cholesterol question, If an infant had a cholesterol level of 800 you may s...

If an infant had a cholesterol level of 800 you may suspect (blank)? How could this happen?d

Gonads and ovary - reproduction, Normal 0 false false false...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

List the different suturing techniques, List the different suturing techniq...

List the different suturing techniques There are a various suturing techniques each suited for a particular situation. Few of the common suturing techniques are: i) Interrup

Logical order in which molecules-atoms are associated, Q What is the logica...

Q What is the logical order in which the concepts of molecules, atoms, cells... up to biosphere are associated? Atoms form molecules that form cells that form tissues that form

How cholera is generally spread in humans, How cholera is generally spread ...

How cholera is generally spread in humans Associated Foods: Cholera is generally a disease spread by poor sanitation, resulting in contaminated water supplies. Sporadic cases o

Guanine, Guanine is one of the nitrogenous bases in the nucleic acids, gua...

Guanine is one of the nitrogenous bases in the nucleic acids, guanine is one of the two purine bases.

The principal nitrogenous excretory in humans, The principal nitrogenous ex...

The principal nitrogenous excretory compound in humans is synthesised: 1. in kidneys but eliminated mostly through liver 2. in kidneys as well as eliminated by kidneys 3.

What are the major biological processes, Q. What are the major biological p...

Q. What are the major biological processes in which calcium participates? Calcium is present in approximately all cells and has various functions. Calcium has an significant ro

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd