Define the fehling's soxhlet method (lane-eynon method), Biology

Assignment Help:

Define the Fehling's Soxhlet method (Lane-Eynon method)?

This is a titrimetric method that is commonly used in food laboratories to estimate percentage of reducing sugars and total sugars. The method is also applicable to biological fluids like milk. The Lane-Eynon method is a titration method of determining the concentration of reducing sugars in a sample. A burette is employed to add the carbohydrate solution being analyzed to a flask consisting of a fixed amount of boiling copper sulfate solution and a methylene blue indicator.

The decreasing sugars in the carbohydrate solution react along with the copper sulfate present in the flask. Once all the copper sulfate in solution has reacted, any further addition of reducing sugars causes the indicator to change from blue to colourless. The volume of sugar solution needed to reach the end point is recorded. Reducing sugars like glucose, fructose, galactose, lactose and maltose can be estimated by direct titration. Non-reducing carbohydrates like sucrose, dextrins and starches cannot be directly estimated by this method. They have to be hydrolyzed into reducing monosaccharides and then estimated titrimetrically. The Fehling Soxhlet method or lane Eynon method is based on oxidation-reduction reaction. You are already familiar with oxidation-reduction tests used for identification of reducing sugars. The basic principle is that sugars in alkaline medium form enediols which reduce cupric ions to cuprous ions. In turn, sugars are oxidized to a mixture of sugar acids. 


Related Discussions:- Define the fehling's soxhlet method (lane-eynon method)

Explain the alanine cycle, The Alanine  cycle Follow the alanine link ...

The Alanine  cycle Follow the alanine link in the alanine cycle. The process goes as under: 1)  Pyruvate formed from glycolysis in  the muscle  is converted to  alanine by t

Amphibians, respiratory system in amphibains

respiratory system in amphibains

Explain the relative nucleotide content of each sample, Can you help explai...

Can you help explain this question in fairly simple terminology? A certain sample of DNA has a Tm of 65oC, while another has a Tm of 58oC. What can you tell about the relative n

What is the function of keratin in the epidermis, What is the function of k...

What is the function of keratin in the epidermis? The epidermis is the outer layer of the skin made of epithelial tissue. In the epidermis there are keratin-secreting cells (ke

Excretion in prawn, EXCRETION IN PRAWN- A pair of antennary gland or gr...

EXCRETION IN PRAWN- A pair of antennary gland or green gland and a median renal sac present. Green glands lie at the base of antennae & open out by renal aperture.

Analysis of the written reports, Analysis of the written reports   of  serv...

Analysis of the written reports   of  service in relation  to  the fitness, quality and efficiency of the service should be provided. The routine record-checking is always insuffic

Define obligatory and facultative water excretion, Define Obligatory and Fa...

Define Obligatory and Facultative water excretion? i) Obligatory water excretion: The kidney is 'obligated' to excrete some water to rid the body of its daily load of urinary s

Symptoms of botulism, Adult Botulism The symptoms of botulism may deve...

Adult Botulism The symptoms of botulism may develop between 12 and 72 hours after the ingestion of toxin containing foods. Symptoms include nausea, vomiting, fatigue, dizzine

How oak trees affect lyme disease in humans, How does the production of aco...

How does the production of acorns by oak trees affect Lyme disease in humans?  When more acorns are produced by oak trees the populations of mice and deer that eat acorns enhan

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd