Define the fehling's soxhlet method (lane-eynon method), Biology

Assignment Help:

Define the Fehling's Soxhlet method (Lane-Eynon method)?

This is a titrimetric method that is commonly used in food laboratories to estimate percentage of reducing sugars and total sugars. The method is also applicable to biological fluids like milk. The Lane-Eynon method is a titration method of determining the concentration of reducing sugars in a sample. A burette is employed to add the carbohydrate solution being analyzed to a flask consisting of a fixed amount of boiling copper sulfate solution and a methylene blue indicator.

The decreasing sugars in the carbohydrate solution react along with the copper sulfate present in the flask. Once all the copper sulfate in solution has reacted, any further addition of reducing sugars causes the indicator to change from blue to colourless. The volume of sugar solution needed to reach the end point is recorded. Reducing sugars like glucose, fructose, galactose, lactose and maltose can be estimated by direct titration. Non-reducing carbohydrates like sucrose, dextrins and starches cannot be directly estimated by this method. They have to be hydrolyzed into reducing monosaccharides and then estimated titrimetrically. The Fehling Soxhlet method or lane Eynon method is based on oxidation-reduction reaction. You are already familiar with oxidation-reduction tests used for identification of reducing sugars. The basic principle is that sugars in alkaline medium form enediols which reduce cupric ions to cuprous ions. In turn, sugars are oxidized to a mixture of sugar acids. 


Related Discussions:- Define the fehling's soxhlet method (lane-eynon method)

Natural control of population growth, N A TURA L CONTROL OF POPULATION G...

N A TURA L CONTROL OF POPULATION GROWTH - 1 .       Biotic potential - It is capacity of population to increase its number by reproduction. For all animals, it is v

DNA, What is replication fork??

What is replication fork??

Determine the floor of nose and nasal spine, Floor of nose and Nasal spine ...

Floor of nose and Nasal spine Although variable, the nasal floor is typically situated 5-9mm below the level of inferior turbinates. The inferior piriform rim is used as a guid

Define liposuction - surgical management for obesity, Define Liposuction - ...

Define Liposuction - surgical management for obesity? Liposuction is a cosmetic surgical procedure different from bariatric surgery. It involves aspiration of subcutaneous fat

Explain fundamental of the photosynthesis process, Q. Is it correct to cons...

Q. Is it correct to consider water decomposition by the action of light the fundamental of the photosynthesis process? In addition ADP photophosphorylation, photic energy is al

How is gas exchange done in flatworms, Q. How is gas exchange done in flatw...

Q. How is gas exchange done in flatworms? Platyhelminthes exchange gases exclusively by diffusion through their body surface. This is only possible because all cells are locali

Hemes and chlorophylls, A haem British English or heme American English is ...

A haem British English or heme American English is a prosthetic group which having of an iron ion contained in the middle of a huge heterocyclic organic ring called as a porphyrin.

What is vacuole , What is Vacuole ? Another structure found only in pla...

What is Vacuole ? Another structure found only in plant cells is the large central vacuole. The vacuole stores enzymes and waste products, in addition to providing the turgor p

Purchasing of service, Normal 0 false false false EN-IN...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

What are the main negative ions found in living beings, What are the main n...

What are the main negative ions found in living beings? The major anions found in living beings are the chlorine anion (Cl-), the phosphate anion (PO4--), the bicarbonate anion

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd