Define the fehling's soxhlet method (lane-eynon method), Biology

Assignment Help:

Define the Fehling's Soxhlet method (Lane-Eynon method)?

This is a titrimetric method that is commonly used in food laboratories to estimate percentage of reducing sugars and total sugars. The method is also applicable to biological fluids like milk. The Lane-Eynon method is a titration method of determining the concentration of reducing sugars in a sample. A burette is employed to add the carbohydrate solution being analyzed to a flask consisting of a fixed amount of boiling copper sulfate solution and a methylene blue indicator.

The decreasing sugars in the carbohydrate solution react along with the copper sulfate present in the flask. Once all the copper sulfate in solution has reacted, any further addition of reducing sugars causes the indicator to change from blue to colourless. The volume of sugar solution needed to reach the end point is recorded. Reducing sugars like glucose, fructose, galactose, lactose and maltose can be estimated by direct titration. Non-reducing carbohydrates like sucrose, dextrins and starches cannot be directly estimated by this method. They have to be hydrolyzed into reducing monosaccharides and then estimated titrimetrically. The Fehling Soxhlet method or lane Eynon method is based on oxidation-reduction reaction. You are already familiar with oxidation-reduction tests used for identification of reducing sugars. The basic principle is that sugars in alkaline medium form enediols which reduce cupric ions to cuprous ions. In turn, sugars are oxidized to a mixture of sugar acids. 


Related Discussions:- Define the fehling's soxhlet method (lane-eynon method)

Explain about the dessicator, Explain about the Dessicator? A dessicato...

Explain about the Dessicator? A dessicator is used to prevent an item from absorbing moisture. It is able to accomplish this because of a chemical, known as the dessicant, is h

Oxidation of enzymes action, Oxidation Some enzymes which have the sulf...

Oxidation Some enzymes which have the sulfhydryl (-SH)  group in  the catalytic site are very sensitive to oxidation. Due  to oxidation of  the  -SH  group  by  aerial  oxygen

Can you explain antibiotics, Q. Can you explain Antibiotics? The antibi...

Q. Can you explain Antibiotics? The antibiotics are the chemicals produced by microorganisms which are able to destroy or inhibit the growth of other microorganisms. The therap

Swine fever, S w i n e fever It is also known as hog cholera and re...

S w i n e fever It is also known as hog cholera and results in high fever and prostration. E t iology : Pestivirus belonging to family Togaviridae is responsi

How is the smoothing tendency of cornea functioned, How is the smoothing te...

How is the smoothing tendency of cornea functioned? Smoothing tendency: In health and in disease condition the epitheliurm has a strong tendency to smoothen the underlyin

What is respiratory system explain their functionality , What is Respirator...

What is Respiratory System explain their functionality ? The respiratory system is responsible for the transport of oxygen and carbon dioxide from outside the body to and from

Filaments of a sarcomere in a skeletal muscle is decreasing, When the overl...

When the overlap between the thin and thick filaments of a sarcomere in a skeletal muscle is decreasing, the A. total length of the I band minus the length of the H zone is dec

Which are the living beings that carry out photosynthesis, Which are the li...

Which are the living beings that carry out photosynthesis? Which is the cell organelle responsible for the absorption of light for the photosynthesis process in plants and algae?

Noise pollution, Sound is a main mean of communication and entertainment an...

Sound is a main mean of communication and entertainment and is also very effective alarm system for some animal. A loud and unwanted or unpleasant sound causes discomfort and is ca

Why organism that can not exist in the presence of oxygen, organism that ca...

organism that can not exist in the presence of oxygen ?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd