Define the fehling's soxhlet method (lane-eynon method), Biology

Assignment Help:

Define the Fehling's Soxhlet method (Lane-Eynon method)?

This is a titrimetric method that is commonly used in food laboratories to estimate percentage of reducing sugars and total sugars. The method is also applicable to biological fluids like milk. The Lane-Eynon method is a titration method of determining the concentration of reducing sugars in a sample. A burette is employed to add the carbohydrate solution being analyzed to a flask consisting of a fixed amount of boiling copper sulfate solution and a methylene blue indicator.

The decreasing sugars in the carbohydrate solution react along with the copper sulfate present in the flask. Once all the copper sulfate in solution has reacted, any further addition of reducing sugars causes the indicator to change from blue to colourless. The volume of sugar solution needed to reach the end point is recorded. Reducing sugars like glucose, fructose, galactose, lactose and maltose can be estimated by direct titration. Non-reducing carbohydrates like sucrose, dextrins and starches cannot be directly estimated by this method. They have to be hydrolyzed into reducing monosaccharides and then estimated titrimetrically. The Fehling Soxhlet method or lane Eynon method is based on oxidation-reduction reaction. You are already familiar with oxidation-reduction tests used for identification of reducing sugars. The basic principle is that sugars in alkaline medium form enediols which reduce cupric ions to cuprous ions. In turn, sugars are oxidized to a mixture of sugar acids. 


Related Discussions:- Define the fehling's soxhlet method (lane-eynon method)

Animals - slow moving waters, Animals - Slow Moving Waters Zooplankton...

Animals - Slow Moving Waters Zooplankton are common here and include an assemblage of protozoa and smaller crustacean, such as water flies, and copepods. Neuston occurring her

Demographic cycle, DEMOGRAPHIC CYCLE - 1 .       First stage - B...

DEMOGRAPHIC CYCLE - 1 .       First stage - B high     - D high     =  0 2 .       Second stage - B high     - D lo w     =  G more eg.

Removal of obturating material -endodontics principles, Removal of obturati...

Removal of obturating material -Gutta percha . -Carrier  bassed gutta perca. -silver points . - paste type. - obstructed fracture instrument .

State the medial pterygoid, Medial pterygoid Muscle bounds the pterygom...

Medial pterygoid Muscle bounds the pterygomandibular space medially which is entered when an inferior alveolar nerve block is administered. Infection of this space is dangerous

Describe primitive anaerobic eukaryotic cells, Q. How can the hypothesis th...

Q. How can the hypothesis that asserts that chloroplasts as well as mitochondria were primitive prokaryotes that related in mutualism with primitive anaerobic eukaryotic cells be c

Explain oxygen concentration - lipid oxidation, Oxygen concentration Wh...

Oxygen concentration When oxygen is abundant, the rate of oxidation is independent of oxygen concentration, but at very low oxygen concentration, the rate is approximately prop

Biostatics, Biostatics :  This is the application of mathematics in living ...

Biostatics :  This is the application of mathematics in living organisms. Biostatistics is a contraction of biology or statistics sometimes it is called as biometry or biometrics.

What is muscular dystrophy, What is Muscular dystrophy Muscular dystro...

What is Muscular dystrophy Muscular dystrophy :- Defects in the muscle due to faulty nutrition.

Physiology of excretion, PHYSIOLOGY OF EXCRETION - 1 .      FORMATION...

PHYSIOLOGY OF EXCRETION - 1 .      FORMATION OF UREA - It takes place in liver. (i) Deamination (ii) Ornithine cycle or urea cycle - Explained by H.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd