Define the fehling's soxhlet method (lane-eynon method), Biology

Assignment Help:

Define the Fehling's Soxhlet method (Lane-Eynon method)?

This is a titrimetric method that is commonly used in food laboratories to estimate percentage of reducing sugars and total sugars. The method is also applicable to biological fluids like milk. The Lane-Eynon method is a titration method of determining the concentration of reducing sugars in a sample. A burette is employed to add the carbohydrate solution being analyzed to a flask consisting of a fixed amount of boiling copper sulfate solution and a methylene blue indicator.

The decreasing sugars in the carbohydrate solution react along with the copper sulfate present in the flask. Once all the copper sulfate in solution has reacted, any further addition of reducing sugars causes the indicator to change from blue to colourless. The volume of sugar solution needed to reach the end point is recorded. Reducing sugars like glucose, fructose, galactose, lactose and maltose can be estimated by direct titration. Non-reducing carbohydrates like sucrose, dextrins and starches cannot be directly estimated by this method. They have to be hydrolyzed into reducing monosaccharides and then estimated titrimetrically. The Fehling Soxhlet method or lane Eynon method is based on oxidation-reduction reaction. You are already familiar with oxidation-reduction tests used for identification of reducing sugars. The basic principle is that sugars in alkaline medium form enediols which reduce cupric ions to cuprous ions. In turn, sugars are oxidized to a mixture of sugar acids. 


Related Discussions:- Define the fehling's soxhlet method (lane-eynon method)

Zooology, green gland is excretory organ of which ortopodic phylem

green gland is excretory organ of which ortopodic phylem

Methods of filter feeding, Methods of Filter Feeding More elaborate me...

Methods of Filter Feeding More elaborate methods of filter feeding are seen in tube dwelling polychaetes which use tentacles to entangle the food particles. Figure shows some

Show some evolutionary advantages of animals, Q. What are some evolutionary...

Q. What are some evolutionary advantages of animals with complete digestive tube? The complete digestive tube permits animals to continuously feed themselves without waiting fo

Illustrate target organs of the hormones, Q. What are target organs of the ...

Q. What are target organs of the hormones? Target tissues, Target organs and target cells are those specific organs, cells and tissues upon which each hormone produces and acts

Adaptation, Adaptation is the tendency of an organism to suit its environm...

Adaptation is the tendency of an organism to suit its environment; one of the main points of Charles Darwin's theory of evolution by the natural selection: organisms adapt to thei

What do you mean by cytotaxonomu and biosustematics, Q. What do you mean by...

Q. What do you mean by Cytotaxonomu and biosustematics? Towards the end of the 19 th century and in the early years of the 20th century, botanists were faced with a problem of

Mitosis, What are the steps of mitosis in order?

What are the steps of mitosis in order?

What is plutonium reprocessing, What is plutonium reprocessing? Why is it a...

What is plutonium reprocessing? Why is it a big environmental issue? Plutonium is the highly radioactive chemical element produced from uranium by nuclear plants. Plutonium can

Explain the chloride permeability, An impermeable membrane separates one li...

An impermeable membrane separates one liter of a 1M KCl solution in the left compartment from one liter of 1M NaCl solution in the right compartment.  At 2 AM today the membrane be

What is endocytosis , Endocytosis is the uptake of extracellular macr...

Endocytosis is the uptake of extracellular macromolecules   across the plasma membrane into the cell. The objects to be ingested is progressively  enclosed by a  small  portion  of

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd