Define the disc electrophoresis, Biology

Assignment Help:

Define the Disc Electrophoresis?

Disc electrophoresis has improved resolution and strong bands are obtained. It requires a two-gel system and several different buffers. The running gel in which the separation takes place is prepared and then overlaid by a short (1 cm), large- pored "Stacking" or "Spacer gel". The buffer in the lower reservoir and in the running gel is as described before, while that in the sample solution and in the stacking gel has a pH about two units less than that of the lower reservoir. When the current is switched on, the buffer ions from the upper reservoir migrate into the stacking gel as the stacking gel buffer ions migrate ahead of them. As this occurs, the upper reservoir buffer ions encounter a pH that is much lower than their pK.

They therefore assume their uncharged (or, in the case of glycine, zwitterionic) form and become electrophoretically immobile. This effect causes the macro-molecular ions to approach the running gel as stacks of very narrow (0.01 nm thick) bands or discs that are ordered according to their mobilities and lie between the migrating ions of the upper reservoir and those of the stacking gel. As the macromolecular ions enter the running gel, they slow down as a result of gel filtration effects. This permits the upper reservoir buffer ions to overtake the macromolecular bands and because of the running gel's higher pH, assume their fully charged form as they too enter the gel and electroporetic separation is achieved.

The compactness of the macromolecular bands entering the running gel greatly increases the resolution of the macromolecular separations. With a study of disc electrophoresis we come to an end of our study about electrophoresis. We hope you did not find the discussion presented in this section too technical. Just for your reference, we shall not be conducting any electrophoresis experiment during our laboratory work for this course, but it is important that you, as a student of dietetics, understand the concept and principle of electrophoresis clearly, since this is one important investigative technique used for research in the field of nutritional biochemistry. We suggest you now answer the questions included in the review question section included herewith. This will help you consolidate your understanding about electrophoresis.

 


Related Discussions:- Define the disc electrophoresis

Explain about the soil morphology, Explain about the Soil morphology S...

Explain about the Soil morphology Soil morphology is the description of the soil body and its general characteristics. The morphology of the soil is expressed by number, kinds

What is the role of platelet plug formation in hemostasis, What is the role...

What is the role of platelet plug formation in hemostasis? What events occur during platelet plug formation? Describe the circumstances that may induce inappropriate platelet plug

How long is the incubation period of the hiv, How long is the incubation pe...

How long is the incubation period of the HIV? What is meant by acute AIDS? The incubation period of the HIV (the time interval among the infection and the beginning of the immu

Techniques of plant tissue culture, Techniques of Plant Tissue Culture ...

Techniques of Plant Tissue Culture A standard tissue culture laboratory should provide facilities for washing and storage of glass ware, preparation and storage of nutrient

Define serum vitamin a content, Define Serum Vitamin A Content? Assessm...

Define Serum Vitamin A Content? Assessment of serum vitamin A content is the most reliable criterion Tor assessing vitamin A status. Serum levels indicative of various degrees

What is kingdom plantae or how organism included in plantae, What is Kingdo...

What is Kingdom Plantae and what organism included in Plantae? Kingdom Plantae :  includes organisms that are eukaryotic, multicellular, photosynthetic. Their cells also cont

What is the auditory tube and its function, Q. What is the auditory tube an...

Q. What is the auditory tube and its function? Auditory tube (Eustachian tube) connects each middle ear to the throat. This tube conducts air between tympanic cavity and outsid

The rapeutic nurse, THE RAPEUTIC NURSE: Development of  therapeutic  n...

THE RAPEUTIC NURSE: Development of  therapeutic  nurse patient  relationship is the basic requirement  of nursing. A helping relationship  in nursing may be explained as an in

What are plankton and benthos, What are plankton, nekton and benthos? P...

What are plankton, nekton and benthos? Plankton, nekton and benthos are the three groups into which aquatic living beings may be separated. The plankton is formed by the alg

Explain root pressure, Explain root pressure? How can you demonstrate it ex...

Explain root pressure? How can you demonstrate it experimentally? What is the cytological basis of Down's syndrome? Give two physical symptoms of this disorder. Describe why the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd