Define the beer''s law - nutritional biochemistry, Biology

Assignment Help:

Define the Beer's Law - Nutritional  Biochemistry?

Beer's law states that the concentration of a substance is directly proportional to the amount of light absorbed or inversely proportional to the logarithm of the transmitted light.

Mathematically, Beer's law is expressed as

A = abc

Where,

A = Absorbance

a = Proportionality constant defined as absorptivity

b = Light path in centimeters

c = Concentration of the absorbing compound, usually expressed in grams per liter

This equation forms the basis of quantitative analysis by absorption photometry.

Absorbance values have no units; hence, the units for a are the reciprocal of those for b and c. When b is 1 cm and c is expressed in moles per liter, the symbol ∈ (epsilon) is substituted for the constant a. The value for  ∈ is a constant for a given compound at a given wavelength under prescribed conditions of solvent, temperature, pH, etc., and is called the molar absorptivity. Values for ∈ are useful to characterize compounds, establish their purity, and compare sensitivities of measurements obtained on derivatives.


Related Discussions:- Define the beer''s law - nutritional biochemistry

Silent myocardial ischaemia, Q. Silent Myocardial Ischaemia? Ans. ...

Q. Silent Myocardial Ischaemia? Ans. This deals primarily with those who have never had symptoms recognized as being of cardiac origin. Others, who have had recognized myo

Cell:the unit of life, cell wall of prokaryotic cell consist of what???

cell wall of prokaryotic cell consist of what???

Define the effect of zinc deficiency, Define the effect of Zinc Deficiency?...

Define the effect of Zinc Deficiency? Zinc deficiency was identified for the first time in 1940 when malnourished Chinese patients were found to have low concentrations of zinc

What is echocardiography, Q. What is Echocardiography? Echocardiography...

Q. What is Echocardiography? Echocardiography in paediatric cardiology forms a different category from that of adult echo with acquired heart disease. Some of views which pro

Define role of vitamin d in controlling gene expression, Define role of Vit...

Define role of Vitamin D in controlling gene expression? Vitamin D is a secosteroid that is metabolically activated and degraded through the actions of three cytochrome P450 hy

Various definitions of species, In biology, a species is smallest and basic...

In biology, a species is smallest and basic unit of classification. Each species is placed within a single genus. For example biological name of human being is homosapiens. Here ho

Hazard analysis, Hazard Analysis Two steps recognized as preliminary a...

Hazard Analysis Two steps recognized as preliminary and detailed analysis of hazard are taken. Preliminary Hazard Analysis (PHA) serves two aims: (i) It can expedite bringi

Study of embryology, Embryology This is the study of growth, fertilizatio...

Embryology This is the study of growth, fertilization, division or differential of the zygote into embryo of early development of living beings before the attainment of structure

Unified cell theory, Unified Cell Theory The present unified cell theo...

Unified Cell Theory The present unified cell theory states that All living things are composed of either a single Cell or of the cells and cell products. The cell is a m

Structures of a flowering plant, Which structures of a flowering plant give...

Which structures of a flowering plant give rise to (a) potatoes, (b) the fleshy scales of an onion?   (a) A potato is a tuber formed at the end of an underground stem (b) O

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd