Define the agarose gel electrophoresis (age), Biology

Assignment Help:

Define the Agarose gel electrophoresis (AGE)?

This technique is used to analyze serum proteins, haemoglobin variants, lactate dehydrogenase isoenzymes, lipoprotein fractions and other substances. The advantages of agarose gel include its lower affinity for proteins and its native clarity after drying which permits excellent densitometric examination. Usually 0.5 to 1.0 g of agarose per decilitre of buffer provides a gel of desired strength and with good migration properties. The sample volume required for AGE is relatively less (0.6-3/ul), and electrophoresis time is relatively short (30-90 minutes depending on experimental conditions).


Related Discussions:- Define the agarose gel electrophoresis (age)

Define end and the beginning of the first interphase period, Q. What are th...

Q. What are the events that mark the end and the beginning of the first interphase period? What happens inside the cell in this period? The initial interphase period is the G1.

Xerophytes, Xerophytes Xerophytes are sometimes loosely defineri as 'pl...

Xerophytes Xerophytes are sometimes loosely defineri as 'plants of dry habitats'. But others have defined xerophytes as 'plants which grow on the substratum that usually beco

Phosphorylation of fructose-6-phosphate, Phosphorylation  of  fructose-6-...

Phosphorylation  of  fructose-6-phosphate Phosphorylation  of  fructose-6-phosphate: This  is  an irreversible reaction, catalyzed byphosphofructo72Snase,  (PFK-  I) a rate-l

Explain control of oedema in nutritional care, Explain Control of Oedema in...

Explain Control of Oedema in Nutritional Care? Low levels of circulating proteins lead to oedema due to loss of colloidal osmotic pressure to maintain the normal fluid shift me

Cyclins and cyclin-dependent kinases, Rhythmic fluctuations in the abundanc...

Rhythmic fluctuations in the abundance and activity of cell-cycle control molecules pace the events of the cell cycle. • Kinase - a protein which activates or deactivates another

Define the single cell proteins (scp), Define the Single Cell Proteins (SCP...

Define the Single Cell Proteins (SCP)? You may have heard of SCP. What is a single cell protein? Let's find out. The term SCP was coined by Prof. Caroll Wilson (MIT) in 1966. I

Illustrate theory of goldman in corneal transparency, Illustrate the theory...

Illustrate the theory of Goldman according to corneal transparency. Goldman's Theory: The fibrils are small in relation to light and do not interfere with light transmissi

What is connective tissue proper, What is connective tissue proper? The...

What is connective tissue proper? The name connective tissue proper is used to assign the connective tissue that fills interstitial spaces as opposed to the specialized connect

Define the sequence-specific dna binding, Which of the following statements...

Which of the following statements regarding the interactions among sequence-specific DNA binding domains and their recognition sites is incorrect? A. Sequence-specific DNA bind

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd