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Define the Agarose gel electrophoresis (AGE)?
This technique is used to analyze serum proteins, haemoglobin variants, lactate dehydrogenase isoenzymes, lipoprotein fractions and other substances. The advantages of agarose gel include its lower affinity for proteins and its native clarity after drying which permits excellent densitometric examination. Usually 0.5 to 1.0 g of agarose per decilitre of buffer provides a gel of desired strength and with good migration properties. The sample volume required for AGE is relatively less (0.6-3/ul), and electrophoresis time is relatively short (30-90 minutes depending on experimental conditions).
what is the effect of insuline in body ?
Which of the following statements about buffers is true? Select one: a. A buffer composed of a weak acid of pKa = 5 is stronger at pH 4 than at pH 6. b. At pH values lower
what are the animals with respiratory organs?
Q. Fluids requirement in ulcerative colitis? Fluids: A liberal intake of fluid should be given to prevent dehydration. The passage of at least 1200 ml of urine indicates that a
Explain Ventilation, Chest Compressions? Even when the first three defibrillation attempts fail, the best chance of resuscitation still lies in successful defibrillation. Hence
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Illustrate the Structure and functions of aqueous humour Aqueous humour is a clear fluid that fills the anterior and posterior chambers of the eye and permeates the vitreous. I
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Q. What is tubal pregnancy? Many times fecundation takes place in the Fallopian tubes in general the newly formed zygote is taken to the uterus where nidation and the embryonic
Which technique would you use to A) see if a particular protein bound to a piece of DNA B) Detect exactly where on a piece of DNA a protein bound.
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