Define the agar shake tube method, Biology

Assignment Help:

Define the Agar Shake Tube Method

The agar shake tube method is useful for isolation of anaerobic microorganisms. What are anaerobic microorganisms? You may recall the theory course that anaerobic microorganisms are those which grow best in the absence of free oxygen. Few examples of anaerobic microorganisms include: Clostridium perfringens, C. botulinum, Bacillus cereus, Campylobacter, E. coli etc.

In preparing pure culture using agar shake tube method, the dilutions of the mixed microbial suspension are made in tubes of molten agar. The colonies formed are embedded in the agar medium. The isolated colony from the highest dilution is used to make further dilution in a new set of molten agar, resulting in a pure culture.


Related Discussions:- Define the agar shake tube method

Briefly explain about phase of the plasmodium life cycle, Q. To which phase...

Q. To which phase of the plasmodium life cycle do the typical chills and fever of malaria correspond? The typical fever and chills episodes of malaria correspond to the phase w

Diagrams, excretary organ of whale

excretary organ of whale

Cryo preserved allografts -surgical techniques, Cry o Preserved Allograf...

Cry o Preserved Allografts (Homograft) :  As these are not mounted, a free hand suturing in two layers has to be done. The valve is thawed by protocol. The septal muscle i

What is the rule for the pairing of nitrogen-containing base, Q. What is th...

Q. What is the rule for the pairing of nitrogen-containing bases in the DNA molecule? And in the RNA? Is this the last question appropriate? The rule for the pairing of nitroge

Spoilage by fungi and bacteria, Q. Explain Spoilage by fungi and bacteria? ...

Q. Explain Spoilage by fungi and bacteria? The dominant spoilage organisms in fruits are fungi, as their pH is low (normally 5),whereas the pH of vegetables is fiom 5.0 to 7

Heliozoans - protozoan, Heliozoans - Protozoan Heliozoans are spherica...

Heliozoans - Protozoan Heliozoans are spherical protozoan that occur in the sea or in still bodies of fresh water. They are mainly located in the bottom debris. Fine needle li

What is left ventricular hypertrophy, What is Left Ventricular Hypertrophy ...

What is Left Ventricular Hypertrophy (LVH) ? LVH develops as the response of the heart to chronic pressure or volume overload and is defined as a left ventricular mass exceedin

Functional significance of this structural difference, The vertebral bodies...

The vertebral bodies are much larger in the lower back than the neck. What is the functional significance of this structural difference?

Define the qualitative technique in nutritional biochemistry, Define the Qu...

Define the Qualitative Technique in Nutritional Biochemistry? In this manual you will be familiarized with simple qualitative techniques and tests to identify compounds such as

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd