Define standard titration for estimation of reducing sugar, Biology

Assignment Help:

Define Standard Titration for estimation of reducing sugar by Fehling Soxhlet Method?

Pipette 5 ml of Fehling's A and 5 ml of Fehling B solution into a 100 ml conical flask. Add approximately 8-9 ml standard glucose solution from a burette so that about 40-45 mg of glucose are added to the flask (to reduce 10 ml of Fehling's solution about 50 mg of glucose are required). Heat the flask on wire gauze till it starts boiling. After about 30-40 seconds add 1 ml of 0.2% methylene blue solution. Continue titratation by adding sugar solution from the burette a few drops at a time and try to finish the titration in 1 minute. At the end point the methylene blue turns colourless. The remaining solution is reddish brown. Repeat the titration till concordant values are obtained. Calculate the amount of glucose required to reduce 10 ml of Fehling's solution and standardize the Fehling's solution.


Related Discussions:- Define standard titration for estimation of reducing sugar

Constrictive pericarditis-pericardial diseases, Constrictive Pericarditis :...

Constrictive Pericarditis :  It is usually the end stage of inflammatory process involving pericardium. In developing world, infection with mycobacterium tuberculosis is still com

Ecology and ecosystem, Ecology and Ecosystem The concepts of environme...

Ecology and Ecosystem The concepts of environment, ecology, ecosystem, energy flow and nutrient cycling in the foundation course on Science and Technology. As you are aware ec

Enhancer, The enhancer is the cis-acting nucleotide series to which the tra...

The enhancer is the cis-acting nucleotide series to which the transcription factor(s) bind, and which increases transcription of the gene. It is not part of the promoter; the gener

Explain an important strategy of group meetings, Explain An important strat...

Explain An important strategy of group meetings An important strategy could be to conduct small group meetings for behavioural change encouraging full participation. The group

The denver development screening test (ddst), THE DENVER DEVELOPMENT SCREEN...

THE DENVER DEVELOPMENT SCREENING TEST (DDST) The Denver Development Screening Test is a standardized test to assess gross motor, fine motor, language and social development

Phylum protozoa, What is the classification of this phylum

What is the classification of this phylum

What is coronary disease, What is coronary disease? Coronary disease, o...

What is coronary disease? Coronary disease, or coronary insufficiency, is a disease in which there is total or partial obstruction of one or more of the arteries that irrigate

Sectioned and formed meats, S e c t i o ned and Formed Meats Thes...

S e c t i o ned and Formed Meats These are technically speaking restructured products, since they are partially disassembled and then reassembled to form products resembl

What dna and rna are polymers composed are called, DNA and RNA are polymers...

DNA and RNA are polymers composed of repeated units called?

Define nutritional requirements of fats and oils, Define Nutritional Requir...

Define Nutritional Requirements of Fats and Oils? As with the other macronutrients, we do compute requirements of visible fats too for all age groups and give guidelines for se

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd