Define standard titration for estimation of reducing sugar, Biology

Assignment Help:

Define Standard Titration for estimation of reducing sugar by Fehling Soxhlet Method?

Pipette 5 ml of Fehling's A and 5 ml of Fehling B solution into a 100 ml conical flask. Add approximately 8-9 ml standard glucose solution from a burette so that about 40-45 mg of glucose are added to the flask (to reduce 10 ml of Fehling's solution about 50 mg of glucose are required). Heat the flask on wire gauze till it starts boiling. After about 30-40 seconds add 1 ml of 0.2% methylene blue solution. Continue titratation by adding sugar solution from the burette a few drops at a time and try to finish the titration in 1 minute. At the end point the methylene blue turns colourless. The remaining solution is reddish brown. Repeat the titration till concordant values are obtained. Calculate the amount of glucose required to reduce 10 ml of Fehling's solution and standardize the Fehling's solution.


Related Discussions:- Define standard titration for estimation of reducing sugar

Zoology, give an account of specific characters of phylum porifera

give an account of specific characters of phylum porifera

Kinds of ovules, Kinds of Ovules Ovules undergo varied degrees of cur...

Kinds of Ovules Ovules undergo varied degrees of curvature during development. The position of micropyle with respect to the funiculus becomes different and constitutes the b

Bovine ephemeral fever, B o v i ne ephemeral fever Ephemeral fever ...

B o v i ne ephemeral fever Ephemeral fever is commonly known as 'three-day sickness'. It affects mainly cattle and occasionally sheep in India. Causative agent is a mosquit

Define how to prevent the cancer, Define How to Prevent the Cancer? A c...

Define How to Prevent the Cancer? A continuing and urgent need is there to take all steps to prevent cancer. Cancer rates are set to increase another 50 percent by the year 202

Respiration – protozoan, Respiration – Protozoan Gas exchange occurs b...

Respiration – Protozoan Gas exchange occurs by the diffusion of oxygen across the cell membrane. Some protozoan utilize this oxygen but are also capable of anaerobic respirati

Carbon dioxide concentration in photosynthesis process, Q. Why is carbon di...

Q. Why is carbon dioxide concentration a limiting factor of the photosynthesis process? And When the carbon dioxide concentration is increased indefinitely is photosynthesis also i

Avian leukosis (sarcoma group of retroviruses), A v i a n leukosis (Sar...

A v i a n leukosis (Sarcoma group of retroviruses) This is a complex of viral diseases caused by an avian retrovirus with various manifestations such as lymphoid leukosis,

For non-insulin users consumption of alcohol, Q. For non-insulin users cons...

Q. For non-insulin users consumption of Alcohol? 1. Substitute for fat calories. A drink providing 100 calories should be substituted by 2 fat exchanges. . 2. Avoid if your

What are the major biological processes, Q. What are the major biological p...

Q. What are the major biological processes in which calcium participates? Calcium is present in approximately all cells and has various functions. Calcium has an significant ro

Explain adverse effects of fosamprenavir calcium, Explain adverse effects o...

Explain adverse effects of fosamprenavir calcium   Adverse effects are similar to those with amprenavir, but in clinical studies the incidence of nausea, vomiting and severe ra

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd