Define standard titration for estimation of reducing sugar, Biology

Assignment Help:

Define Standard Titration for estimation of reducing sugar by Fehling Soxhlet Method?

Pipette 5 ml of Fehling's A and 5 ml of Fehling B solution into a 100 ml conical flask. Add approximately 8-9 ml standard glucose solution from a burette so that about 40-45 mg of glucose are added to the flask (to reduce 10 ml of Fehling's solution about 50 mg of glucose are required). Heat the flask on wire gauze till it starts boiling. After about 30-40 seconds add 1 ml of 0.2% methylene blue solution. Continue titratation by adding sugar solution from the burette a few drops at a time and try to finish the titration in 1 minute. At the end point the methylene blue turns colourless. The remaining solution is reddish brown. Repeat the titration till concordant values are obtained. Calculate the amount of glucose required to reduce 10 ml of Fehling's solution and standardize the Fehling's solution.


Related Discussions:- Define standard titration for estimation of reducing sugar

Commensalism, #quesWhat benefits can commensalism offer to a species..

#quesWhat benefits can commensalism offer to a species..

What are fossil fuels, What are fossil fuels? Fossil fuels, like oil, g...

What are fossil fuels? Fossil fuels, like oil, gas and coal, form when organic material is preserved from the complete action of decomposers, generally buried deep and under pr

Explain fixed performance system in oxygen therapy, Explain fixed performan...

Explain fixed performance system in oxygen therapy? Fixed Performance Systems: These devices are capable of delivering a fixed, preset concentration of oxygen regardless of t

Explain different amino acids found in proteins, How many different protein...

How many different proteins, each composed of 8 amino acids, can be constructed using the 20 different amino acids found in proteins?

Indication in circulatory assist devices, Indication :  IABP is commonly u...

Indication :  IABP is commonly used to supporting failing left ventricle after open-I1ca1-t surgery or in cardiogenic shock after myocardial infarction. It is at times used in uns

Explain the sarcomere, For a sarcomere of a skeletal muscle, define the fol...

For a sarcomere of a skeletal muscle, define the following terms: A is the length of the A Band; H is the length of the H Zone; I is the total length of the I Bands in the sarcomer

Define some precautions for estimation of amount of bacteria, Define some P...

Define some Precautions for Estimation of the Amount of Bacteria? 1. Dilution should be made carefully. 2. Use fresh sterile pipette for making each dilution. 3. Aseptic

#tzoology, #affinities of ctenophora with platyhelmintheson..

#affinities of ctenophora with platyhelmintheson..

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd