Define standard titration for estimation of reducing sugar, Biology

Assignment Help:

Define Standard Titration for estimation of reducing sugar by Fehling Soxhlet Method?

Pipette 5 ml of Fehling's A and 5 ml of Fehling B solution into a 100 ml conical flask. Add approximately 8-9 ml standard glucose solution from a burette so that about 40-45 mg of glucose are added to the flask (to reduce 10 ml of Fehling's solution about 50 mg of glucose are required). Heat the flask on wire gauze till it starts boiling. After about 30-40 seconds add 1 ml of 0.2% methylene blue solution. Continue titratation by adding sugar solution from the burette a few drops at a time and try to finish the titration in 1 minute. At the end point the methylene blue turns colourless. The remaining solution is reddish brown. Repeat the titration till concordant values are obtained. Calculate the amount of glucose required to reduce 10 ml of Fehling's solution and standardize the Fehling's solution.


Related Discussions:- Define standard titration for estimation of reducing sugar

What is facultative anaerobic beings, What is the difference between facult...

What is the difference between facultative anaerobic beings and obligate anaerobic beings? Facultative anaerobic beings, such as the fungi Saccharomyces cerevisiae, a brewing y

What is the mn blood system, What is the MN blood system? What is the patte...

What is the MN blood system? What is the pattern of genetic inheritance of the MN blood system? A MN blood system is a third (in addition to the ABO and the Rh) system of blood

Vaccine against tuberculosis, Q. Is there vaccine against tuberculosis? ...

Q. Is there vaccine against tuberculosis? The vaccine against the tuberculosis is called as BCG (bacillus Calmette-Guérin). BCG is not used in a few countries where tuberculosi

Explain two divisions of meiosis, Q. What are the two divisions of meiosis ...

Q. What are the two divisions of meiosis and what are the major events that occur in those divisions? Meiosis is divided into first meiotic division, or meiosis I, and second m

Nutrition, how do carnivorous feed

how do carnivorous feed

What is prokaryotic chromosomes, The DNA of a bacterial cell,such as Escher...

The DNA of a bacterial cell,such as Escherichia coli is a circular double-stranded molecule  often  referred  to as the bacterial  chromosome.  The circular  DNA  is packaged into

Discuss the importance of septa in maxillary sinus, Discuss the importance ...

Discuss the importance of septa in maxillary sinus during a sinus lift or graft surgery. Septa, when present in the maxillary sinus will divide it into sections creating separa

What do you mean by respiratory pigments, Q. What are respiratory pigments?...

Q. What are respiratory pigments? What are some respiratory pigments and in which animal groups can each of them be found? Respiratory pigments are molecules present in the blo

List various liver function tests, Question 1 List various liver function ...

Question 1 List various liver function tests. Discuss the importance of each test. Add a note on standardization of various liver function tests Question 2 Discuss the follow

How is water importance to life, How is water's importance to life reflecte...

How is water's importance to life reflected in the normal range of internal pH values that living things are likely to have?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd