Define standard titration for estimation of reducing sugar, Biology

Assignment Help:

Define Standard Titration for estimation of reducing sugar by Fehling Soxhlet Method?

Pipette 5 ml of Fehling's A and 5 ml of Fehling B solution into a 100 ml conical flask. Add approximately 8-9 ml standard glucose solution from a burette so that about 40-45 mg of glucose are added to the flask (to reduce 10 ml of Fehling's solution about 50 mg of glucose are required). Heat the flask on wire gauze till it starts boiling. After about 30-40 seconds add 1 ml of 0.2% methylene blue solution. Continue titratation by adding sugar solution from the burette a few drops at a time and try to finish the titration in 1 minute. At the end point the methylene blue turns colourless. The remaining solution is reddish brown. Repeat the titration till concordant values are obtained. Calculate the amount of glucose required to reduce 10 ml of Fehling's solution and standardize the Fehling's solution.


Related Discussions:- Define standard titration for estimation of reducing sugar

Which is the normal sign of the electric charge, Which is the normal sign o...

Which is the normal sign of the electric charge among the two sides of the neuron plasma membrane? What is the potential difference (voltage) generated among these two sides? What

Evaluate what is the frequency of carriers in a tank, Zebrafish are raised ...

Zebrafish are raised in fish tanks with one family per tank. The mutation spadetail is a recessive lethal. A stock tank for spadetail was made by crossing two carriers. The fish in

What is the original concentration, A sample of yeast cells was serially di...

A sample of yeast cells was serially diluted 1/10 five times then serially diluted 1/3. the number of yeasts was338 yeasts per ml. what was the original concentration? Please show

Limerick, how do we write a limerick spell?

how do we write a limerick spell?

Explain about microwave irradiation, Q. Explain about Microwave irradiation...

Q. Explain about Microwave irradiation? Most micro-organisms are destroyed by the action of microwave of a frequency of about 2450 MHz and a wavelength of 12.24 cm. The water c

Create a dichotomous key for ant, Create a dichotomous key the following or...

Create a dichotomous key the following organisms, ant, butterfly, fly, beetle, grass hopper, wasp, ladybug, roach and dragonfly.

Determine what is the Cell Cycle, The cell cycle undergoes a sequence of ch...

The cell cycle undergoes a sequence of changes which involve a period of growth replication of DNA, Followed by cell division. This sequence of changes is called cell cycle.

Define stage - parts of the microscope, Define Stage - Parts of the Microsc...

Define Stage - Parts of the Microscope? It is a fixed platform positioned about halfway up the arm and provide surface for keeping the slide. Slide is held in position either b

Enumerate various factors controlling osseointegration, Q. Enumerate variou...

Q. Enumerate various factors controlling osseointegration? The factors controlling osseointegration are: i) Occlusal load ii) Biocompatibility of the material iii) Imp

Zoonoses disease-contagious ecthyma/orf , Contagious ecthyma/ Orf Primaril...

Contagious ecthyma/ Orf Primarily it is a disease of sheep and goats caused by a dermotropic virus - Orf virus - that belongs to the Parapoxvirus genus of the family Poxviridae. T

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd