Define sample titration for saponification number of fats, Biology

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Define Sample Titration for Saponification Number of Fats?

Measure 2 ml of oil with the help of a pipette and transfer this oil to a 250 ml conical flask. Add 40 ml of alcoholic KOH to the flask. Fit a reflux water condenser on the flask. Put a small water bath on a tripod stand and place the flask fitted with the condenser in the water bath. Heat the water bath for 30- 35 minutes until the fat globulesin the flask are not visible. Cool. Add a few drops of phenolphtalein as an indicator and titrate against the standardized HCI.


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