Define role of buffer in electrophoresis, Biology

Assignment Help:

Define Role of Buffer in Electrophoresis?

Buffer ions have a two-fold purpose in electrophoresis, they carry the applied current, and they fix the pH at which electrophoresis is carried out and, thus, determine the kind of electrical charge on the solute and the extent of ionization of the solute. By these effects, the buffer determines the direction of the electrophoretic migration to one of the two electrodes.

The buffer ionic strength determines the thickness of the ionic cloud surrounding the charged molecules and, thus, also the rate of migration and the sharpness of the zones. Many buffer systems are used in electrophoretic procedures, but the most widely used are the barbital buffers and the Tris-boric acid EDTA buffers. Next, let us get to know about the different techniques of electrophoresis.


Related Discussions:- Define role of buffer in electrophoresis

Where in living beings can found mineral salts, Q. Which are mineral salts?...

Q. Which are mineral salts? Where in living beings can found mineral salts? Ans. Mineral salts are simple inorganic substances made of metallic chemical elements, like sodiu

What is mass transportation across the cell membrane, Mass transportation i...

Mass transportation is the access or the exiting of substances in or from the cell engulfed by portions of membrane. The fusion of internal substance-having membranous vesicles wit

Homeostasis, using a specific example describe how hormones have an importa...

using a specific example describe how hormones have an important role in the regulation of body functions and also, what happens if there is an over secretion or an under secretion

Hypoglycaemia, Hypoglycaemia is defined as state of low blood glucose level...

Hypoglycaemia is defined as state of low blood glucose level of less than 50mg/dl. Low blood sugar level varies from person to person. Causes Since hypoglycaemia can occur in

Explain the term- pedon and polypedon, Explain the term- pedon and polypedo...

Explain the term- pedon and polypedon The characteristics of soil do not change vertically downwards only, there are lateral variations also as soil is a three dimensional body.

Contagious bovine pleuropneumonia, C o n t a g i o u s bovine pleu...

C o n t a g i o u s bovine pleuropneumonia It is a disease of bovines characterized by high rise of body temperature and difficulty in respiration. E t iolo

What are the identical proteins, Q. Are proteins with the same number of ea...

Q. Are proteins with the same number of each dissimilar amino acid that forms them necessarily identical proteins? Even if many proteins have the same number of each different

Calculate the electric field at a point midway, Three charges are at the co...

Three charges are at the corners of an equilateral triangle, as shown in the figure below. Calculate the electric field at a point midway between the two charges on the x-axis. (Le

Closed type of circulatory systems, Closed Type of Circulatory Systems ...

Closed Type of Circulatory Systems Some non-chordates, like cephalopods (octopuses, squids), earthworms, polychactes and all vertebrates have a closed circulation along with b

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd