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The general mechanism of RNA synthesis by these eukaryotic RNA polymerases is the same as for the prokaryotic enzyme that is:
? The initiation of RNA synthesis by RNA polymerase is directed through the presence of a promoter site on the 5' side of the transcriptional start site;
? The RNA polymerase transcribes one strand, the antisense (-) strand of the DNA sample;
? RNA is synthesis that does not require a primer;
figure of contractile vacuole of amoeba
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