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Define reagents required and methodology for Benedict Test?
Reagents Required
- Sugar solutions of glucose, fructose, galactose, maltose, lactiose, sucrose and starch.
- Benedict's reagent (containing copper sulphate, sodium citrate and sodium carbonate).
Methodology
To 5 ml of Benedict's reagent in a test tube add 8-10 drops of glucose solution. Observe the test tube carefully. Repeat the test with fructose and galactose.
The Km for an enzyme A. is equal to kcat, measured at low substrate concentration. B. is the substrate concentration that gives a velocity that is half the maximum velocity.
economic importance of viruses and bacteria ?
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