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Define reagents required and methodology for Benedict Test?
Reagents Required
- Sugar solutions of glucose, fructose, galactose, maltose, lactiose, sucrose and starch.
- Benedict's reagent (containing copper sulphate, sodium citrate and sodium carbonate).
Methodology
To 5 ml of Benedict's reagent in a test tube add 8-10 drops of glucose solution. Observe the test tube carefully. Repeat the test with fructose and galactose.
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how the optical rotaion occurs in glucose and ribose?
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