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Define reagent required and methodology for Barfoed Test?
Reagents
- Solutions of glucose, fructose, galactose, lactose, maltose, sucrose and starch
- Barfoed's reagent (copper acetate in glacial acetic acid)
Methodology
Take 5 ml of glucose solution and add 6-10 drops of Barfoed's reagent. Boil for about 1 minute. Observe carefully. Repeat this test taking other carbohydrates.
What is Hemocoel? Explain in detail. Principle body cavity in molluscs and arthropods, remnant of the blastocoel. It forms part of open circulatory system found in these animal
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