Define reagent required and methodology for barfoed test, Biology

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Define reagent required and methodology for Barfoed Test?

Reagents

- Solutions of glucose, fructose, galactose, lactose, maltose, sucrose and starch

- Barfoed's reagent (copper acetate in glacial acetic acid)

Methodology

Take 5 ml of glucose solution and add 6-10 drops of Barfoed's reagent. Boil for about 1 minute. Observe carefully. Repeat this test taking other carbohydrates.

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