Define procedure for detection of number of bacteria in milk, Biology

Assignment Help:

Define Procedure for Detection of Number of Bacteria in Milk?

1. Place a clean, non-greasy slide over the graph paper.

2. Spread 0.01 ml of the milk over an area of 1 cm2 as a thin even film.

3. Dry the smear by gently passing through the flame.

4. Defat the smear by flooding with xylol for 1 minute.

5. Wash the slide with alcohol to remove xylene followed by washing with water to remove the alcohol.

6. Stain the smear with methylene blue for 15-30 second.

7. After washing with water, observe the dried preparation under oil immersion. Count the number of bacteria in about 25 microscopic field.

8. Determine the average number of bacteria per microscopic field and multiply with microscopic factor (500,000) to get bacterial counts per ml of the milk sample.


Related Discussions:- Define procedure for detection of number of bacteria in milk

Cardiac muscles, Normal 0 false false false EN-IN X-N...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Show how adaptation affecting taste quality, Q. Show How Adaptation affecti...

Q. Show How Adaptation affecting taste quality? Adaptation - A low concentration solution will not give any sensation after tasting a higher concentration solution due to the a

Define the density of egg, Define the density of egg  The density of e...

Define the density of egg  The density of egg products is not affected by dehydration. When a dried egg product is reconstituted to its natural solids, it has about the same d

What are the main interspecific ecological interactions, Q. What are the ma...

Q. What are the main interspecific ecological interactions? The major harmonious interspecific ecological interactions are: protocooperation, commensalism and mutualism. The ma

Bacteria, Bacteria The study of bacteria is called bacteriology. Bacte...

Bacteria The study of bacteria is called bacteriology. Bacteria are unicellular organisms and possess a distinct cell wall. They are included in kingdom Prokaryote. The bacter

What is cardiac transplantation , What is Cardiac Transplantation ? ...

What is Cardiac Transplantation ? Cardiac Transplantation :  The first successful clinical heart transplant was done by Christian Bmnard at Cape Town in South Africa in 196

Respiratory systerm, Which two structures does the trachea lead to in the l...

Which two structures does the trachea lead to in the lungs?

Regulation of pyruvate kinase, Regulation of pyruvate kinase, pyruvate carb...

Regulation of pyruvate kinase, pyruvate carboxylase and PEP carboxykinase A) In liver,  pyruvate  kinase  is inhibited  by high levels  of ATP and alanine  so which  glycolysis

Describe atg or aug, ATG or AUG: The codon for the methionine; the transla...

ATG or AUG: The codon for the methionine; the translation initiation codon. generally, protein translation can only start at the methionine codon (although this codon might be fou

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd