Define principle for iodine number estimation - lipids, Biology

Assignment Help:

Define Principle for Iodine Number Estimation - Lipids?

Iodine number is defined as the number of grams of iodine absorbed by 100 grams of fat, it gives an idea of the relative degree of un-saturation of fats or the amount of double bonds present in the fats. Iodine number is directly proportional to the degree of un-saturation.

In actual practice, Iodine monobromide (IBr) (Hanus method) or Iodine monochloride (ICI) (Wij's method) is used in preference to iodine solution, as these are more reactive and give more reliable and reproducible results. The method is based on the absorption of halogen by the unsaturated fatty acids present in fats. The method employs dissolution of fat in chloroform (CHCI3) and treatment with a known excess of halogen solution. After allowing for absorption of the halogen, the excess halogen is liberated as I, and is back titrated against standard sodium thiosulphate solution using starch as indicator. By using an excess of sodium thiosulphite to react with each double bond in the fatty acid chains and measuring the amount used by back titrating, we can determine the amount of sodium thiosulphate used and thus the relative number of double bonds.


Related Discussions:- Define principle for iodine number estimation - lipids

Explain disorders of the gastrointestinal tract, Q. Explain Disorders of th...

Q. Explain Disorders of the gastrointestinal tract? The diseases and disorders of the gastrointestinal (GI) tract. Have you ever suffered from abnormal symptoms of the gastroin

Define reagents for preparation of phosphate buffers, Define Reagents for P...

Define Reagents for Preparation of Phosphate Buffers? Buffer solutions of pH4, pH7 and pH 9.18 phosphate buffer solution: 0.1 M disodium hydrogen phosphate or Na 2 HPO 4

Define intermediate level of the numeric pyramid, In the short term what wi...

In the short term what will happen to the levels above and below a population of secondary consumers of a numeric pyramid if a large number of individuals from this population dies

What is physical accommodation, What is Physical Accommodation Physical...

What is Physical Accommodation Physical Accommodation is the actual physical deformation in the lens power. It is measured in diopters, e.g., if the focussing power of the eye

Categories of benthic flowering plants, Categories of benthic flowering pla...

Categories of benthic flowering plants Zone of emergent plants Consisting of plants whose roots and stems remain submerged in water and whose upper leaves and ste

Dispersal of seeds , Dispersal of Seeds A plant usually bears many fr...

Dispersal of Seeds A plant usually bears many fruits and innumerable seeds. If all the seeds produced by a plant were to germinate in the immediate vicinity, this will have s

Blood pressure, BLOO D PRESSURE Is the result of the sum of  ...

BLOO D PRESSURE Is the result of the sum of                 (i) Osmotic colloidal pressure of blood                 (ii) Elastic recoil of blood vessel's wall.

What are the two reactants, what are the two reactants and the two products...

what are the two reactants and the two products of a dehydration reaction/ What are the two reactants and products of a hydrolysis reaction

Progressive senescence - senescence, Progressive senescence - Senescence ...

Progressive senescence - Senescence This is the third category of senescence. Here also the leaves are shed but it is gradual senescence of leaves up the stem. For example, th

Alcohol related emergencies, Alcohol Related Emergencies: Alcohol is o...

Alcohol Related Emergencies: Alcohol is one of  the most serious health problems. Alcoholism is defined as a primary or chronic disease with genetic, psychological and environ

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd