Define principle for iodine number estimation - lipids, Biology

Assignment Help:

Define Principle for Iodine Number Estimation - Lipids?

Iodine number is defined as the number of grams of iodine absorbed by 100 grams of fat, it gives an idea of the relative degree of un-saturation of fats or the amount of double bonds present in the fats. Iodine number is directly proportional to the degree of un-saturation.

In actual practice, Iodine monobromide (IBr) (Hanus method) or Iodine monochloride (ICI) (Wij's method) is used in preference to iodine solution, as these are more reactive and give more reliable and reproducible results. The method is based on the absorption of halogen by the unsaturated fatty acids present in fats. The method employs dissolution of fat in chloroform (CHCI3) and treatment with a known excess of halogen solution. After allowing for absorption of the halogen, the excess halogen is liberated as I, and is back titrated against standard sodium thiosulphate solution using starch as indicator. By using an excess of sodium thiosulphite to react with each double bond in the fatty acid chains and measuring the amount used by back titrating, we can determine the amount of sodium thiosulphate used and thus the relative number of double bonds.


Related Discussions:- Define principle for iodine number estimation - lipids

What are deciduous trees, What are deciduous trees? The Deciduous trees...

What are deciduous trees? The Deciduous trees are plants that lose their leaves in a period of the year and in the case of the deciduous trees of the temperate forest the fall

Write the factorson on which metaboilc changes depend, The metaboilc change...

The metaboilc changes would depend on the  following  factors: a)  Nature of infection b)  Severity of the infection c)  Duration of the infection d)  Presence or abse

Physical state of protoplasm, PHYSICAL STATE OF PROTOPLASM Several theo...

PHYSICAL STATE OF PROTOPLASM Several theories have been given about its physical structure - (i )  Granular Theory (Proposed by Altman, Hanstein, 1886) - Granules embed

0smosis, what is the difination of osmosis

what is the difination of osmosis

Name the nitrogenous waste excreted in the larval, What benefit does the se...

What benefit does the sea anemone get in the sea anemone-hermit crab facultative mutualism? Give an alternative term for this part of mutualism. Name the nitrogenous wast

Zoogly, are protozoans primitive to life .

are protozoans primitive to life .

Heart in amphibians, Amphibia n - 3 chambered heart 2 auricles and ...

Amphibia n - 3 chambered heart 2 auricles and 1 ventricle. Sinus venosus and truncus arteriosus (its main part is pylengium) present. Incomplete double circulation prese

Explain the torsional failure - root canal test, Explain the Torsional Fail...

Explain the Torsional Failure - Root Canal Test a. Occurs when the tip of the rotating instrument blinds (locked) in the canal while the motor continues to rotate the shank of

Proteins of animal origin - egg, Proteins of Animal Origin - Egg Eggs: ...

Proteins of Animal Origin - Egg Eggs:  Roughly, the chicken egg consists of 11% shell, 31% yolk and 58% white. Liquid whole egg consists of 65% white and 35% yolk. The primary

Dietary considerations oesophagitis, Q. Dietary considerations oesophagitis...

Q. Dietary considerations oesophagitis? The dietary considerations for this condition are the same as in oesophag it is. The patient should be recommended to: • eat smaller

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd