Define principle for iodine number estimation - lipids, Biology

Assignment Help:

Define Principle for Iodine Number Estimation - Lipids?

Iodine number is defined as the number of grams of iodine absorbed by 100 grams of fat, it gives an idea of the relative degree of un-saturation of fats or the amount of double bonds present in the fats. Iodine number is directly proportional to the degree of un-saturation.

In actual practice, Iodine monobromide (IBr) (Hanus method) or Iodine monochloride (ICI) (Wij's method) is used in preference to iodine solution, as these are more reactive and give more reliable and reproducible results. The method is based on the absorption of halogen by the unsaturated fatty acids present in fats. The method employs dissolution of fat in chloroform (CHCI3) and treatment with a known excess of halogen solution. After allowing for absorption of the halogen, the excess halogen is liberated as I, and is back titrated against standard sodium thiosulphate solution using starch as indicator. By using an excess of sodium thiosulphite to react with each double bond in the fatty acid chains and measuring the amount used by back titrating, we can determine the amount of sodium thiosulphate used and thus the relative number of double bonds.


Related Discussions:- Define principle for iodine number estimation - lipids

Can you explain balloon valvuloplasty, Q. Can you explain Balloon Valvulopl...

Q. Can you explain Balloon Valvuloplasty? Whenever feasible valvuloplasty is the treatment modality of choice. One has to make sure that the valve is suitable and expertise to

Effects of long term malnutrition, Effects of long term malnutrition ...

Effects of long term malnutrition The effects of long term malnutrition are more severe in children. Children do not have sufficient amounts of carbohydrates and fats in

Positive and negative regulation, Normal 0 false false fals...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4 The lac operon is an

Define nutrition counseling - management of eating disorders, Define Nutrit...

Define Nutrition Counseling - Management of Eating Disorders? Nutrition counseling can be used to accomplish a variety of goals, such as reducing behaviours related to the eati

What is viscosity?, Q. What is viscosity? Viscosity is resistance to fl...

Q. What is viscosity? Viscosity is resistance to flow of a liquid. It is a measure of the resistance of a fluid to deformation under shear stress. It is commonly perceived as "

Describe paradoxical and reverse split s in second heart, Describe Paradoxi...

Describe Paradoxical and Reverse Split S in second heart sound? Sequence of seminar valve closure reversed i.e. P, proceeds A. A reversed split is nearly always due to delayed

Categories of air pollutants, Categories of Air Pollutants From the ab...

Categories of Air Pollutants From the above list you can see that the air pollutants can be broadly classified into the following two categories: Primary Pollutants

Where are the adrenal glands located, Where are the adrenal glands located?...

Where are the adrenal glands located? How many are they and what are their portions? Each adrenal gland is located on the top of every kidney (forming a hat-like structure for

Sequence of cardiac activation, Sequence of Cardiac Activation Depolar...

Sequence of Cardiac Activation Depolarisation is initiated by an impulse form the SA node. Impulse spreads through both atria. Inter-atrial conduction and atrial myocardial

To test the effects of temperature on the activity of yeast, To test the ef...

To test the effects of temperature on the activity of yeast Make up a quantity of bread dough .Stir a yeast cake in water and methodically mix the yeast with the dough. Divide

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd