Define principle for iodine number estimation - lipids, Biology

Assignment Help:

Define Principle for Iodine Number Estimation - Lipids?

Iodine number is defined as the number of grams of iodine absorbed by 100 grams of fat, it gives an idea of the relative degree of un-saturation of fats or the amount of double bonds present in the fats. Iodine number is directly proportional to the degree of un-saturation.

In actual practice, Iodine monobromide (IBr) (Hanus method) or Iodine monochloride (ICI) (Wij's method) is used in preference to iodine solution, as these are more reactive and give more reliable and reproducible results. The method is based on the absorption of halogen by the unsaturated fatty acids present in fats. The method employs dissolution of fat in chloroform (CHCI3) and treatment with a known excess of halogen solution. After allowing for absorption of the halogen, the excess halogen is liberated as I, and is back titrated against standard sodium thiosulphate solution using starch as indicator. By using an excess of sodium thiosulphite to react with each double bond in the fatty acid chains and measuring the amount used by back titrating, we can determine the amount of sodium thiosulphate used and thus the relative number of double bonds.


Related Discussions:- Define principle for iodine number estimation - lipids

How light reaction effects cell metabolism, How light reaction effects Cell...

How light reaction effects Cell Metabolism ? Light reactions :  The light reactions are carried out by two separate assemblies of several hundred chlorophyll molecules, acces

Pre and post-operative nursing care of a child, Pre-operative and Post-oper...

Pre-operative and Post-operative Nursing Care of a Child with Cleft Lip: We shall begin with pre-operative nursing care and then focus on post-operative care. As you know that

What event marks the beginning of the menstrual cycle, What event marks the...

What event marks the beginning of the menstrual cycle? What is the blood concentration of FSH, LH, estrogen and progesterone in this phase of the cycle? By convention the menst

When to use the term 2'' carbon, When do you use the term 2' carbon and whe...

When do you use the term 2' carbon and when do you use 2 carbons on a molecular structure? I need examples please!

Infants with maple syrup urine disease, Q. Infants with maple syrup urine d...

Q. Infants with maple syrup urine disease? Infants with MSUD appear normal at birth. Once they are fed on protein containing feed, they start showing the classic symptoms. Neur

List the goals of targeting blood pressure in diabetics, List the goals of ...

List the goals of targeting blood pressure in diabetics. Goals of targeting blood pressure in diabetics are:  Patients with diabetes should be treated to a systolic blood pr

Explain the types of fats, Jillian and Michael are in their freshman year a...

Jillian and Michael are in their freshman year at college. The two have been friends since grade school and the two of them enjoy getting together for dinner. Michael typically

Determine the use of natural colourants, Determine the use of natural colou...

Determine the use of natural colourants The use of natural colourants is limited due to their instability, low tinctorial power or price disadvantage.  The trend towards natura

Illustrate the major cells of the nervous system, Q. Illustrate the major c...

Q. Illustrate the major cells of the nervous system? The major cells of the nervous system are the neurons. as well the neurons the nervous system is also constituted of glial

How to get a stock primer concentration of 100µm, I have 30µg of a primer, ...

I have 30µg of a primer, whose molecular weight is 600. How much water should I add to get a stock primer concentration of 100µM?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd