Define principle for iodine number estimation - lipids, Biology

Assignment Help:

Define Principle for Iodine Number Estimation - Lipids?

Iodine number is defined as the number of grams of iodine absorbed by 100 grams of fat, it gives an idea of the relative degree of un-saturation of fats or the amount of double bonds present in the fats. Iodine number is directly proportional to the degree of un-saturation.

In actual practice, Iodine monobromide (IBr) (Hanus method) or Iodine monochloride (ICI) (Wij's method) is used in preference to iodine solution, as these are more reactive and give more reliable and reproducible results. The method is based on the absorption of halogen by the unsaturated fatty acids present in fats. The method employs dissolution of fat in chloroform (CHCI3) and treatment with a known excess of halogen solution. After allowing for absorption of the halogen, the excess halogen is liberated as I, and is back titrated against standard sodium thiosulphate solution using starch as indicator. By using an excess of sodium thiosulphite to react with each double bond in the fatty acid chains and measuring the amount used by back titrating, we can determine the amount of sodium thiosulphate used and thus the relative number of double bonds.


Related Discussions:- Define principle for iodine number estimation - lipids

How are the triglycerides made, Q. How are the triglycerides made? Trig...

Q. How are the triglycerides made? Triglycerides, oils or fats, are made of one molecule of glycerol bound to three molecules of fatty acids. Hydroxyls of each one of the three

Bacteria entering the blood system, Briefly describe the principal lines of...

Briefly describe the principal lines of defence against bacteria entering the blood system. A blood clot forms a barrier to entry by bacteria. White cells ingest and kill bacte

St-segment in exercise induced pvc, Q. What do you mean by  ST-Segment in ...

Q. What do you mean by  ST-Segment in Exercise Induced  PVC? Ans. Over the years it has been believed that repolarization in ventricular ectopic beats had no diagnostic si

Explain procedure for sub-culturing of a culture, Explain Procedure for Sub...

Explain Procedure for Sub-Culturing of a Culture Now carry out the exercise following the steps given herewith: 1. Sterilize the inoculating loop and needle by heating to re

Define vegetable as a rich source of protein, Define Vegetable as a rich so...

Define Vegetable as a rich source of protein? Fresh vegetables are not considered to be a very good source of proteins. On fresh weight basis, the average protein content of so

Enzymes, what is rennin? how does it work? and what is its optimum pH level...

what is rennin? how does it work? and what is its optimum pH level and activity temperature?

Determine when the homologous chromosomes occur, In which period of meiosis...

In which period of meiosis does the pairing of homologous chromosomes occur? The pairing of homologous chromosomes is an essential step for meiosis because the rightness of the

Explain the body tube of microscope, Explain the Body Tube of Microscope? ...

Explain the Body Tube of Microscope? Above the stage and attached to the arm is the body tube, to which eye piece(s) or ocular lens and revolving nosepiece is attached. Microsc

Plant classfication, why obelia is considered to be of special interest in ...

why obelia is considered to be of special interest in zoology as an animal showing an intermediate grade of organisation

Why is the determination of the blood types of the donor, Why is the determ...

Why is the determination of the blood types of the donor and of the recipient important in transfusions? The Red blood cells have different antigens in the outer surface of the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd