Define principle for estimation of cholesterol - lipids, Biology

Assignment Help:

Define Principle for Estimation of cholesterol - Lipids?

Zlatkis and associates have evolved a method of quantitative determination of cholesterol based on a reagent containing ferric chloride, glacial acetic acid and concentrated sulphuric acid. Cholesterol gives a purple colour with this reagent. The colour development is due to a dehydration reaction in the cholesterol molecule to form 3, 5 cholestadiene which is oxidized by H2SO4 and polymerises to form a diamer or trimer. The cholastadiene and its polymers react with H2SO4 to form mono and disulfonic acids which are highly coloured. In the presence of added metal ions like ferric, the disulphonic acids are preferentially formed. The detailed step-by-step procedure for cholesterol estimation is included in Experiment 3 later in this practical. Study the procedure carefully before conducting the experiment. With this basic understanding about iodine number, saponification technique and methods for cholesterol estimation, we are well equipped to carry out the experiments given in this practical. There are three experiments in this practical.

 


Related Discussions:- Define principle for estimation of cholesterol - lipids

What proportion of the progeny, In holly, serrated leaves are dominant to s...

In holly, serrated leaves are dominant to smooth-edged leaves, and red berries are dominant to green berries. Two holly plants heterozygous for leaf edge shape and berry color are

Illustrate meiotic division, Q. In which meiotic division does the separati...

Q. In which meiotic division does the separation of the homologous take place and what are the ploidies of the generated cells after the end of that process? The separation of

Which blood vessels carry venous blood to the heart, Q. What are the blood ...

Q. What are the blood vessels that carry venous blood to the heart? What is the blood vessel that collects arterial blood from the heart? The blood vessels that debouch in the

Discuss refractive and response to wound functions of cornea, Discuss the r...

Discuss the refractive and response to wound functions of cornea. Refractive Function: Cornea, through its interaction with the tear film, forms a smooth refractive functi

Chromosomes with spindle fiber, Chromosomes with spindle fiber: The kineto...

Chromosomes with spindle fiber: The kinetochore fibers of spindle attach to the kinetochore region (specialized area in centromere) of chromosome, and align them at the equator of

Can stroke be prevented, Can stroke be prevented Brain experts are conv...

Can stroke be prevented Brain experts are convinced that several strokes can be prevented with proper attention to lifestyle factors which increase risk, including smoking, exc

Brain thinking, in what form brain stores thinking or past experiences?

in what form brain stores thinking or past experiences?

The codon gca specifies which amino acid, Use the genetic code table Assign...

Use the genetic code table Assignment34 The codon GCA specifies which amino acid?

Pcr, What is pcr?

What is pcr?

What are diastolic compressive forces, Q. What are Diastolic Compressive Fo...

Q. What are Diastolic Compressive Forces? Coronary perfusion pressure equal to pressure gradient between the coronary arteries and the pressure in LV in diastole. When coronary

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd