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Define Most Probable Number (MPN) Method?
The MPN method is like agar shake tube method where no agar is used. The dilutions of the microbial suspension are made in liquid medium till the final tube in a series shows no growth. By repeating this process several times pure culture can be obtained. The method is used for estimation of number of microorganisms in foods, waste water and other samples.
Explain Procedure for Gram Staining of Bacterial Cultures? Now carry out the exercise following the steps enumerated herewith. 1. Prepare bacterial smear on a clean, non-gre
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Tissue Valves : Tissue valves mounted on a frame with a sewing ring are inserted by the same technique as used in the case of prosthetic valves.
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