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Define Most Probable Number (MPN) Method?
The MPN method is like agar shake tube method where no agar is used. The dilutions of the microbial suspension are made in liquid medium till the final tube in a series shows no growth. By repeating this process several times pure culture can be obtained. The method is used for estimation of number of microorganisms in foods, waste water and other samples.
It is immune difficult mediated hypersensitivity. Typical manifestations contain rheumatoid arthritis, serum sickness, necrotizing etc.
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