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Define Most Probable Number (MPN) Method?
The MPN method is like agar shake tube method where no agar is used. The dilutions of the microbial suspension are made in liquid medium till the final tube in a series shows no growth. By repeating this process several times pure culture can be obtained. The method is used for estimation of number of microorganisms in foods, waste water and other samples.
give an example invertebrate phylum/phyla for protoplasmic grade organization
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Under which form is nitrogen fixed by living beings? Most living beings cannot use molecular nitrogen to get nitrogen atoms. Producers fix nitrogen mostly from nitrate (NO3-).
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