Define method used for capsular staining - anthony staining, Biology

Assignment Help:

Define method Used for Capsular Staining - Anthony Staining Method?

Another method used for capsular staining is Anthony staining method, devised by E.E. Anthony in 1931. The method involves the use of a primary stain i.e., 1% aqueous crystal violet. Both the capsular material and the cell wall will take the colour of the stain and will appear dark blue. But the capsule being nonionic will not absorb the primary stain. It adheres to the capsule without binding to it. Because capsule is water soluble, therefore, copper sulphate is used both as a decolourizing agent and as a counter stain. It takes out the colour from the capsular material and counter stains the decolourized capsule which now appears as a light blue zone surrounding deep purple cells. In both Klebsiella and Bacillus, capsule can be seen surrounding the dark purple coloured cells. Capsule will appear as a clear zone in negative staining against dark background, whereas, it is light blue coloured in anthony's method.

 


Related Discussions:- Define method used for capsular staining - anthony staining

What is diabetes mellitus, Question 1 What is diabetes mellitus? Discuss b...

Question 1 What is diabetes mellitus? Discuss briefly its pathogenesis. Explain how would you diagnose this disorder Question 2 Discuss the following Glucose tolerance

Fortification to prevention of iron deficiency anaemia, Define Fortificatio...

Define Fortification to prevention of iron deficiency anaemia? Food fortification is one of the alternatives that ensure consumption of the nutrient regularly in daily diet. Fo

Explain sialography, Question 1 Explain Sialography? How will you perfo...

Question 1 Explain Sialography? How will you perform the same? Question 2 How will you set up your radiology department to take up the IVU studies? Question 3 D

Define effect on oestrogens and progesterone in pregnancy, Define effect on...

Define effect on Oestrogens and progesterone in pregnancy? Oestrogens and progesterone are secreted by the placenta from 8-10 weeks of gestation. Progesterone increases through

How is lymph propelled through the lymphatics, How is lymph propelled throu...

How is lymph propelled through the lymphatics? Some of the larger lymphatics are capable to contract, or else the lymph is propelled by body muscles which contract and 'squash'

Investigations process of acute pericarditis, Q. Investigations process of ...

Q. Investigations process of acute pericarditis? 1) Blood Examination Erythrocyte sedementaion rate (ESR) may be elevated in tuberculous; collagen and purulent pericarditis.

Define briefly about the selenium, Define briefly about the Selenium? T...

Define briefly about the Selenium? The element selenium was discovered in 1817 in association with the element sulphur. However, selenium as an essential nutrient remained unre

Blood flow, if i start in the superior mesenteric vain where do i have to g...

if i start in the superior mesenteric vain where do i have to go through to get to the left anterior circumflex humeral artery. by the way of the hepatic portal vein and the heart

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd