Define method used for capsular staining - anthony staining, Biology

Assignment Help:

Define method Used for Capsular Staining - Anthony Staining Method?

Another method used for capsular staining is Anthony staining method, devised by E.E. Anthony in 1931. The method involves the use of a primary stain i.e., 1% aqueous crystal violet. Both the capsular material and the cell wall will take the colour of the stain and will appear dark blue. But the capsule being nonionic will not absorb the primary stain. It adheres to the capsule without binding to it. Because capsule is water soluble, therefore, copper sulphate is used both as a decolourizing agent and as a counter stain. It takes out the colour from the capsular material and counter stains the decolourized capsule which now appears as a light blue zone surrounding deep purple cells. In both Klebsiella and Bacillus, capsule can be seen surrounding the dark purple coloured cells. Capsule will appear as a clear zone in negative staining against dark background, whereas, it is light blue coloured in anthony's method.

 


Related Discussions:- Define method used for capsular staining - anthony staining

Respiratory organ, . which is the main part of respiratory organ?

. which is the main part of respiratory organ?

Zoology, #question.what is protostomous and deuterostomes development.

#question.what is protostomous and deuterostomes development.

Agro-industrial byproducts, Agro-industrial byproducts Agro-industrial...

Agro-industrial byproducts Agro-industrial by-products in Southeast Asia are available in plenty, due to the emphasis on crop cultivation. By-products of agro-industries repre

Define the role of vitamin a in immune response, Define the Role of Vitamin...

Define the Role of Vitamin A in immune response? Vitamins A is essential to normal immune function and- regulation. As discussed above, during vitamin A deficiency the goblet c

Give examples of diseases caused by bacteria, Give three examples of diseas...

Give three examples of diseases caused by bacteria, Diseases caused by bacteria contain tonsillitis, blood poisoning, scarlet fever, tuberculosis, typhoid, diphtheria, food poi

Response to wounding, Response to Wounding Wounding of tomato plants b...

Response to Wounding Wounding of tomato plants by mechanical injury or chewing by insects releases a factor that triggers accumulation of two proteinase inhibitors-throughout

Thalassemia, Thalassemia   Thalassemias are the commonest hemolytic ana...

Thalassemia   Thalassemias are the commonest hemolytic anaemias or disorder seen in children in India. This is characterised by deficiency  in the synthesis  of one of the norm

What are the cardiac muscles, What are the Cardiac muscles We know tha...

What are the Cardiac muscles We know that the heart muscles (cardiac muscles) are not under the control of  mind. The day we are born, it starts its activity and the day peopl

What is the capillarity, What is the capillarity? How this phenomenon chemi...

What is the capillarity? How this phenomenon chemically explained? What is relevance of the capillarity for water transport in plants? The Capillarity is the phenomenon through

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd