Define method used for capsular staining - anthony staining, Biology

Assignment Help:

Define method Used for Capsular Staining - Anthony Staining Method?

Another method used for capsular staining is Anthony staining method, devised by E.E. Anthony in 1931. The method involves the use of a primary stain i.e., 1% aqueous crystal violet. Both the capsular material and the cell wall will take the colour of the stain and will appear dark blue. But the capsule being nonionic will not absorb the primary stain. It adheres to the capsule without binding to it. Because capsule is water soluble, therefore, copper sulphate is used both as a decolourizing agent and as a counter stain. It takes out the colour from the capsular material and counter stains the decolourized capsule which now appears as a light blue zone surrounding deep purple cells. In both Klebsiella and Bacillus, capsule can be seen surrounding the dark purple coloured cells. Capsule will appear as a clear zone in negative staining against dark background, whereas, it is light blue coloured in anthony's method.

 


Related Discussions:- Define method used for capsular staining - anthony staining

Bjork-shiley valve (b-s)-types of valves, Bjork-Shiley Valve (B-S) : This...

Bjork-Shiley Valve (B-S) : This is one of the earliest types of tilting disc valves introduced in early 70's. The earlier models like concavo-convex disc valves were withdrawn fr

Define feeding for infants of hiv/aids mothers, Define Feeding for Infants ...

Define Feeding for Infants of HIV/AIDS Mothers? It is recommended to continue breast-feeding even if the disease will continue to be transmitted. If these infants are not breas

Classification of chromatography -basic separation technique, Define Classi...

Define Classification of Chromatography - Basic Separation Technique Chromatography can be classified depending upon: Physiochemical principles/factors, The techniqu

Embryonic development, Embryonic Development The embryonic stage exte...

Embryonic Development The embryonic stage extends from the second week by the 8th week and is charactrised by formation of placenta, the development of internal organs and ap

Cranium, need images of cranium

need images of cranium

Environmental pollution, The earth's environment has been composed in a way...

The earth's environment has been composed in a way which sustains life on this planet. Through on process, the different constituents of the atmosphere are consumed and through the

Cytokinins - plant growth substances, Cytokinins - Plant Growth Substances ...

Cytokinins - Plant Growth Substances Folke Skoog observed that the cell division or differentiation is affected by AMP and other purines. A nucleic acid from yeast was also fo

Biogeochemical cycling - nutrient cycles, Biogeochemical Cycling - Nutrient...

Biogeochemical Cycling - Nutrient Cycles The energy flows through ecosystems enabling the organisms to perform various kinds of work, and is ultimately lost as heat. It is gon

Respiration in animals, Respiration Robert Boyel and Robert Hook fir...

Respiration Robert Boyel and Robert Hook first of all explained the meaning of respiration. Main aim of respiration is to liberate energy in the form of A.T.P. Oxidati

Show complications of mitral stenosis, Q. Show Complications of Mitral Sten...

Q. Show Complications of Mitral Stenosis ? Hemoptysis It is a common complication in patients with mitral stenosis and is related to the severity of mitral stenosis. Pulm

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd