Define method used for capsular staining - anthony staining, Biology

Assignment Help:

Define method Used for Capsular Staining - Anthony Staining Method?

Another method used for capsular staining is Anthony staining method, devised by E.E. Anthony in 1931. The method involves the use of a primary stain i.e., 1% aqueous crystal violet. Both the capsular material and the cell wall will take the colour of the stain and will appear dark blue. But the capsule being nonionic will not absorb the primary stain. It adheres to the capsule without binding to it. Because capsule is water soluble, therefore, copper sulphate is used both as a decolourizing agent and as a counter stain. It takes out the colour from the capsular material and counter stains the decolourized capsule which now appears as a light blue zone surrounding deep purple cells. In both Klebsiella and Bacillus, capsule can be seen surrounding the dark purple coloured cells. Capsule will appear as a clear zone in negative staining against dark background, whereas, it is light blue coloured in anthony's method.

 


Related Discussions:- Define method used for capsular staining - anthony staining

Complications of exchange transfusion, Complications of Exchange Transfusio...

Complications of Exchange Transfusion i) Infection of umbilical vessels and systemic injection related to blood transfusion like malaria, CMV, AIDS and hepatitis B. ii) O

How different are fecundation in osteichthyes, How different are fecundatio...

How different are fecundation in osteichthyes and in chondrichthyes? In chondrichthyes fecundation is internal by means of copulation. In osteichthyes fecundation generally is

Define the disease - marcus gunn pupil, Define the disease - Marcus Gunn Pu...

Define the disease - Marcus Gunn Pupil (RAPD) Relative Afferent Pupillary Defect occurs in a pupil of an eye that is blirld from optic nerve disease. When light is shifted to t

Representatives of the echinoderm phylum, Q. What are some representatives ...

Q. What are some representatives of the echinoderm phylum? Starfishes, sea urchins, sea cucumbers (holothurians) and brittle stars are examples of echinoderms.

Phylum apicomplexa - protozoan, Phylum Apicomplexa - Protozoan Charact...

Phylum Apicomplexa - Protozoan Characteristic set of organelles (apical complex) asssociated with the anterior end present in some developmental stages. Cilia and flagella abs

Concept mapping, Mrs. Jackson is a 76 year old woman living in a skilled nu...

Mrs. Jackson is a 76 year old woman living in a skilled nursing facility. She has Hx: CVA with left-sided weakness, incontinent of bowel and bladder, PEG tube, GERD, HTN, Dementia,

What is the meaning of trocophore, What is the meaning of Trocophore? T...

What is the meaning of Trocophore? This free-swimming ciliated larval stage is found in numerous animal phyla including Mollusca and Annelida. Larvae has a unique circle of pre

Polynephritis in cattle, Polynephritis in cattle Contagious bovine pol...

Polynephritis in cattle Contagious bovine polynephritis is a specific infection of urinary tract of cattle. The causative agent is Corynebacterium renale, a rod shaped gram-po

What is the vector of malaria, What is the vector of malaria? How different...

What is the vector of malaria? How different is its behavior from the behavior of the vector of dengue fever? The vector of malaria is a mosquito of the genus Anopheles, also k

What is cdna libraries, A cDNA library is made by using the reverse transcr...

A cDNA library is made by using the reverse transcriptase of a retrovirus  to synthesize cDNA (complementary  DNA)  copies  of the total mRNA from a cell (or perhaps a subfraction

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd