Define method used for capsular staining - anthony staining, Biology

Assignment Help:

Define method Used for Capsular Staining - Anthony Staining Method?

Another method used for capsular staining is Anthony staining method, devised by E.E. Anthony in 1931. The method involves the use of a primary stain i.e., 1% aqueous crystal violet. Both the capsular material and the cell wall will take the colour of the stain and will appear dark blue. But the capsule being nonionic will not absorb the primary stain. It adheres to the capsule without binding to it. Because capsule is water soluble, therefore, copper sulphate is used both as a decolourizing agent and as a counter stain. It takes out the colour from the capsular material and counter stains the decolourized capsule which now appears as a light blue zone surrounding deep purple cells. In both Klebsiella and Bacillus, capsule can be seen surrounding the dark purple coloured cells. Capsule will appear as a clear zone in negative staining against dark background, whereas, it is light blue coloured in anthony's method.

 


Related Discussions:- Define method used for capsular staining - anthony staining

Explain about the light sense, Explain about the Light sense You  must ...

Explain about the Light sense You  must be  aware that when you  enter  into a cinema hall, you  are unable  to  see  and  find  out your  seat  initially for  a few minutes; b

Signs and symptoms diabetic ketoacidosis, Q. Signs and Symptoms diabetic ke...

Q. Signs and Symptoms diabetic ketoacidosis? The following are the signs and symptoms of DKA: 1) Increased heart rate (tachycardia) 2) Low blood pressure 3) Dehydratio

Procedure for use microbiological laboratory equipment, Define Procedure fo...

Define Procedure for use Microbiological Laboratory Equipments? Now carry out this activity following the steps given herewith: 1. Observe each instrument (as mentioned abov

Effects of noise - noise pollution, Effects of Noise - Noise Pollution ...

Effects of Noise - Noise Pollution Noise can affect in the following three ways: Interferes with communication, Diminishes hearing and Affects health and even

How to produce a similar sickling effect, Which of the following amino acid...

Which of the following amino acids would you expect to produce a similar sickling effect if placed at position 6? Select all that apply. 1. Arginine 2. Leucine 3. Lysine

Define the replication of dna strands, Which of the following is a true sta...

Which of the following is a true statement regarding the replication of DNA strands? A. The products of replication contain one double helix made exclusively of parental DNA an

Explain the control of air pollution, Explain the Control of Air Pollution ...

Explain the Control of Air Pollution a. Air belongs to everyone Need it to treat air pollution as public problem   b. Air pollution is an inevitable concomitan

Which aqueous solution has the lowest ph, The aqueous solution with the low...

The aqueous solution with the lowest pH is: Select one: a. 0.01 M HCl. b. 0.1 M acetic acid (pKa = 4.86). c. 0.1 M formic acid (pKa = 3.75). d. 0.1 M HCl. e. 10 t

Movement of molecules into and out of a cell, how does a plasma membrane re...

how does a plasma membrane regulate movement of molecules into and out of a cell? is it polarity, integrity, permeability, or solubility? these are my choices

Explain starch, Starch It is a plant polysaccharide synthesized by  the...

Starch It is a plant polysaccharide synthesized by  the plant by photosynthesis and stored mainly  in  grains,  legumes,  roots  and  tubers.  Its molecular formula is (C 6 H 1

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd