Define method used for capsular staining - anthony staining, Biology

Assignment Help:

Define method Used for Capsular Staining - Anthony Staining Method?

Another method used for capsular staining is Anthony staining method, devised by E.E. Anthony in 1931. The method involves the use of a primary stain i.e., 1% aqueous crystal violet. Both the capsular material and the cell wall will take the colour of the stain and will appear dark blue. But the capsule being nonionic will not absorb the primary stain. It adheres to the capsule without binding to it. Because capsule is water soluble, therefore, copper sulphate is used both as a decolourizing agent and as a counter stain. It takes out the colour from the capsular material and counter stains the decolourized capsule which now appears as a light blue zone surrounding deep purple cells. In both Klebsiella and Bacillus, capsule can be seen surrounding the dark purple coloured cells. Capsule will appear as a clear zone in negative staining against dark background, whereas, it is light blue coloured in anthony's method.

 


Related Discussions:- Define method used for capsular staining - anthony staining

Explain about the nutrition security, Explain about the Nutrition Security?...

Explain about the Nutrition Security? Food security is a part of the broader concept of nutrition security. A household can be said to be nutritionally secure if it is able to

Mammalian testis, Normal 0 false false false EN-IN X-...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Natural selection, Darwin put fort the notion of the survival of the fittes...

Darwin put fort the notion of the survival of the fittest. How do we define 'fittest'? The answer usually given is those who survive. So you can readily see that this is a circular

Explain precautions for performing completed coliform test, Explain Precaut...

Explain Precautions for Performing the Completed Coliform Test 1. Aseptic conditions should be adopted while performing the experiment. 2. Destaining is the critical step in

Planning the nursing care for infective endocarditis, Planning the Nursing ...

Planning the Nursing Care   Provide bed rest  Administer antibiotics as advised Prevent infection  Implementation of Nursing Care  Provide Bed Rest   Sin

Discuss in detail about the close head injuries, Discuss in detail about th...

Discuss in detail about the Close Head Injuries Closed-head injuries result from a blow to the head, which can subject the brain to a variety of mechanical forces: Damag

Enumerate the principles of suturing, Enumerate the principles of suturing ...

Enumerate the principles of suturing Principles of Suturing are as follows: - Sutures should always be inserted through the more mobile tissue flap first. - A circular fo

Define proteins as biological buffers, Define Proteins as biological buffer...

Define Proteins as biological buffers? Proteins have the ability to accept or donate hydrogen ions and by doing so they serve as biological buffers. In blood, there are three i

Spore forming protozoan, Normal 0 false false false EN-...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Nutritional requirements of fats and oils during infancy, Define Nutritiona...

Define Nutritional Requirements of Fats and Oils during Infancy? Adequate breast-feeding ensures 30 g fat intake by infants, of which 10% is linoleic acid and 1% is linolenic a

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd