Define method used for capsular staining - anthony staining, Biology

Assignment Help:

Define method Used for Capsular Staining - Anthony Staining Method?

Another method used for capsular staining is Anthony staining method, devised by E.E. Anthony in 1931. The method involves the use of a primary stain i.e., 1% aqueous crystal violet. Both the capsular material and the cell wall will take the colour of the stain and will appear dark blue. But the capsule being nonionic will not absorb the primary stain. It adheres to the capsule without binding to it. Because capsule is water soluble, therefore, copper sulphate is used both as a decolourizing agent and as a counter stain. It takes out the colour from the capsular material and counter stains the decolourized capsule which now appears as a light blue zone surrounding deep purple cells. In both Klebsiella and Bacillus, capsule can be seen surrounding the dark purple coloured cells. Capsule will appear as a clear zone in negative staining against dark background, whereas, it is light blue coloured in anthony's method.

 


Related Discussions:- Define method used for capsular staining - anthony staining

Transportation of gases in tracheophytes vascular tissues, Is the transport...

Is the transportation of gases in tracheophytes made through the vascular tissues? The Carbon dioxide and The Oxygen are not transported through the xylem or phloem. These gase

Define the structure of hemicellulose, Structure of Hemicellulose When ...

Structure of Hemicellulose When compared with cellulose, it has lower degree of polymerization, more readily soluble in acid and alkali and non-fibrous. On hydrolysis, it predo

Plant tissues responsible for the supporting of the plant, Which are plant ...

Which are plant tissues responsible for the supporting of the plant? The plant supporting tissues are the sclerenchyma and the collenchyma. The collenchyma is made of elonga

Pollination, how pollination occurs in Salvia?

how pollination occurs in Salvia?

State the principles of behaviour change communication, Principles of behav...

Principles of behaviour change communication - BCC should be integrated with program goals from the starting. BCC is a necessary part of diabetes prevention, care and support p

Explain early diastolic sound in diastolic, Explain Early Diastolic Sound i...

Explain Early Diastolic Sound in diastolic ? a) Opening snap (0s) of Rheumatic MS: High frequency sound. Generation of sound: Because of high LA pressure, the superior systolic

Role of echocardiography, Echocardiography is a very important tool in diag...

Echocardiography is a very important tool in diagnosis of cardiovascular diseases and is used  virtually in all categories of cardiovascular diseases. There are certain areas where

Structure of the intestines, Describe the structure of the intestines. How ...

Describe the structure of the intestines. How are they modified to carryout their functions? How does the human intestine compare to the fetal pig?

Explain the importance of cellulose, Importance of cellulose About one ...

Importance of cellulose About one third of the world's production of  purified cellulose is  used as the base material for a number of water-soluble derivatives with predesigne

Explain about the magnesium - macro minerals, Explain about the Magnesium -...

Explain about the Magnesium - macro minerals? Magnesium (Mg) ranks fourth in overall abundance in body among the cations. It is also the least abundant among macro minerals, th

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd