Define method used for capsular staining - anthony staining, Biology

Assignment Help:

Define method Used for Capsular Staining - Anthony Staining Method?

Another method used for capsular staining is Anthony staining method, devised by E.E. Anthony in 1931. The method involves the use of a primary stain i.e., 1% aqueous crystal violet. Both the capsular material and the cell wall will take the colour of the stain and will appear dark blue. But the capsule being nonionic will not absorb the primary stain. It adheres to the capsule without binding to it. Because capsule is water soluble, therefore, copper sulphate is used both as a decolourizing agent and as a counter stain. It takes out the colour from the capsular material and counter stains the decolourized capsule which now appears as a light blue zone surrounding deep purple cells. In both Klebsiella and Bacillus, capsule can be seen surrounding the dark purple coloured cells. Capsule will appear as a clear zone in negative staining against dark background, whereas, it is light blue coloured in anthony's method.

 


Related Discussions:- Define method used for capsular staining - anthony staining

Mode of nutrition , What is the mode of nutrition in fish,human,amoeba,scor...

What is the mode of nutrition in fish,human,amoeba,scorpian & toad ?

Simple or unilayered epithelia, SIMPL E EPITHELIA (UNILAYERED EPITHELIA) -...

SIMPL E EPITHELIA (UNILAYERED EPITHELIA) - 1 .      SIMPLE SQUAMOUS EPITHELIUM (PAVEMENT EPITHELIUM) - It is composed of large flat cells whose edges fit closely toge

Population growth - population parameters, Population Growth - Population P...

Population Growth - Population Parameters and Regulation The size of a population depends upon the balance between natality and immigration through which individuals are added

Define isomerases, Isomerases These  enzymes (code  EC 5)  catalyze geo...

Isomerases These  enzymes (code  EC 5)  catalyze geometric  or  structural  changes  within  a molecule. According  to the  type of  isomerism,  they  may be  called  racemases

Why vasa efferentia are the ductules, Vasa efferentia are the ductules lead...

Vasa efferentia are the ductules leading from: 1. Testicular lobules to rete testis 2. Rete testis to vas deferens 3. Vas deferens to epididymis 4. Epididymis to urethra

Contraceptive methods, Contraceptive methods The safe and easy methods ...

Contraceptive methods The safe and easy methods to prevent pregnancy are called contraceptive methods. i) Contraceptive methods followed by females are : 1) Use of harmon

Describe the technique of food processing, Technique of food processing ...

Technique of food processing Freezing is another ancient technique of food processing, which allows the transportation of perishable food items for long distances from producti

Nonsurgical retreatment -secondary endodontic ttt, Nonsurgical retreatment ...

Nonsurgical retreatment (secondary endodontic ttt ) -Is the main difference between primary endodontic disease versus post treatment disease is the need to Regain access to

Classification levels of blood pressure, Table gives the current classifica...

Table gives the current classification of hypertension as recommended by JNC VII. The classification is based on the mean of two or more seated readings on two or more occasions.

Mollusca, what is phylum mollusca?

what is phylum mollusca?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd