Define method used for capsular staining - anthony staining, Biology

Assignment Help:

Define method Used for Capsular Staining - Anthony Staining Method?

Another method used for capsular staining is Anthony staining method, devised by E.E. Anthony in 1931. The method involves the use of a primary stain i.e., 1% aqueous crystal violet. Both the capsular material and the cell wall will take the colour of the stain and will appear dark blue. But the capsule being nonionic will not absorb the primary stain. It adheres to the capsule without binding to it. Because capsule is water soluble, therefore, copper sulphate is used both as a decolourizing agent and as a counter stain. It takes out the colour from the capsular material and counter stains the decolourized capsule which now appears as a light blue zone surrounding deep purple cells. In both Klebsiella and Bacillus, capsule can be seen surrounding the dark purple coloured cells. Capsule will appear as a clear zone in negative staining against dark background, whereas, it is light blue coloured in anthony's method.

 


Related Discussions:- Define method used for capsular staining - anthony staining

Nervous system - spinal cord, SPINAL CORD (MYELON) It is part of CNS. 4...

SPINAL CORD (MYELON) It is part of CNS. 45 cm long (in woman 43 cm) & weight 35 gms. Origi n from lower part of medulla oblongeta. Shape - Long cylindrical,

What is parasitism, What is parasitism? Parasitism is the ecological in...

What is parasitism? Parasitism is the ecological interaction in which a being lives at the expense of another. The parasite often does not cause immediate death of the host sin

Botulism, B o tu l i s m It is a toxicity in chickens, turkeys, d...

B o tu l i s m It is a toxicity in chickens, turkeys, ducks and other aquatic birds caused by a bacterial toxin produced by anaerobic bacteria Clostridium botulinum mai

Homeostasis., drawandlabelthemajorendocrineglandsinthehumanbody

drawandlabelthemajorendocrineglandsinthehumanbody

Class arachnida of phylum arthropoda, Class Arachnida of Phylum Arthropoda ...

Class Arachnida of Phylum Arthropoda Body divided into cephalothorax and abdomen. Cephalothorax along with four (4) pairs of legs; abdomen segmented or unsegmented, with or wi

Criteria for identifying biodiversity hot spots, A simple method used to id...

A simple method used to identify areas of high conservation priority is the selection of countries according to highest species richness. Among the top 12 countries identified this

Widal test is used for the diagnosis, Widal test is used for the diagnosis ...

Widal test is used for the diagnosis of: 1. Malaria 2. Pneumonia 3. Tuberculosis 4. Typhoid Typhoid

What is eucalyptus dive, To verify the effect of intra-specific competition...

To verify the effect of intra-specific competition on the growth of saplings of Eucalyptus dives, an experiment was designed in which two sets of pots were used. In the first set o

Which is not produced by the hypothalamus, Which of the following is NOT pr...

Which of the following is NOT produced by the hypothalamus? A) releasing hormones B) inhibiting hormones C) antidiuretic hormone D) oxytocin E) thyroid-stimulating h

Describe what are non degradable pollutants, Question : (a) Briefly de...

Question : (a) Briefly describe what are non degradable pollutants? (b) What do you meant by ichthyosarcotoxic fishes and give two examples of ichthyosarcotoxic fish poiso

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd