Define method used for capsular staining - anthony staining, Biology

Assignment Help:

Define method Used for Capsular Staining - Anthony Staining Method?

Another method used for capsular staining is Anthony staining method, devised by E.E. Anthony in 1931. The method involves the use of a primary stain i.e., 1% aqueous crystal violet. Both the capsular material and the cell wall will take the colour of the stain and will appear dark blue. But the capsule being nonionic will not absorb the primary stain. It adheres to the capsule without binding to it. Because capsule is water soluble, therefore, copper sulphate is used both as a decolourizing agent and as a counter stain. It takes out the colour from the capsular material and counter stains the decolourized capsule which now appears as a light blue zone surrounding deep purple cells. In both Klebsiella and Bacillus, capsule can be seen surrounding the dark purple coloured cells. Capsule will appear as a clear zone in negative staining against dark background, whereas, it is light blue coloured in anthony's method.

 


Related Discussions:- Define method used for capsular staining - anthony staining

Best relates the severity and frequency of natural events, In general, whic...

In general, which of the following best relates the severity and frequency of natural events, such as storms or droughts? A) Nothing is known about this relationship. B) The severi

How many larvae per treatment, A study is made to verify the effects of pes...

A study is made to verify the effects of pesticide exposure on pupal weight of butterflies. In a pilot study, 5 randomly selected larvae are raised on plants exposed to the pestici

Hormones controlling other functions, Hormones Controlling Other Functions ...

Hormones Controlling Other Functions Crustacean's exhibit pronounced capacity for physiological color changes. It is known that the color changing mechanisms in Crustacea are

What do you mean by systematic zoology, Q. What do you mean by Systematic Z...

Q. What do you mean by Systematic Zoology ? It contains articles on animals systematic, and papers dealing with cytological attributes of species and higher taxa, distribution p

Gregor mendel''s law of independent segregation, Gregor Mendel's Law of Ind...

Gregor Mendel's Law of Independent Segregation referred to which of the following genetic elements? A. Alleles of two genes that reside on the similar chromosome B. Alleles

Which pathways produce atp via slp, How substrate-level phosphorylation (SL...

How substrate-level phosphorylation (SLP) differs from oxidative phosphorylation. Which pathways produce ATP via SLP? Where in cell oxidative phosphorylation (prokaryotes/eukaryote

Mouth examination of new born, Mouth Inspect and palpate the lips, ...

Mouth Inspect and palpate the lips, gums, tongue, palate and oropharynx. Rule out cleft lip and palate, size of chin if small and receding it  shows micrognathia ind

Mixed mitral stenosis and regurgitation, Mixed Mitral Stenosis and Regurgit...

Mixed Mitral Stenosis and Regurgitation The most common cause for a combined lesion is rheumatic, Very rarely; it could be of congenital origin. Regurgitation in a stenotic val

Determine about the accommodation of eye, Determine about the Accommodation...

Determine about the Accommodation of eye Accommodation is the ability of the overall refracting power of the eye to change to clearly view objects at different distances. This

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd