Define method used for capsular staining - anthony staining, Biology

Assignment Help:

Define method Used for Capsular Staining - Anthony Staining Method?

Another method used for capsular staining is Anthony staining method, devised by E.E. Anthony in 1931. The method involves the use of a primary stain i.e., 1% aqueous crystal violet. Both the capsular material and the cell wall will take the colour of the stain and will appear dark blue. But the capsule being nonionic will not absorb the primary stain. It adheres to the capsule without binding to it. Because capsule is water soluble, therefore, copper sulphate is used both as a decolourizing agent and as a counter stain. It takes out the colour from the capsular material and counter stains the decolourized capsule which now appears as a light blue zone surrounding deep purple cells. In both Klebsiella and Bacillus, capsule can be seen surrounding the dark purple coloured cells. Capsule will appear as a clear zone in negative staining against dark background, whereas, it is light blue coloured in anthony's method.

 


Related Discussions:- Define method used for capsular staining - anthony staining

Definition of breach of contract, Question 1: Mention the types of IRBs...

Question 1: Mention the types of IRBs. If the IRB disapproves clinical study, can the sponsor approach another IRB? Justify your answer. Mention different types of IRBs

How many people carry the allele, Phenylketonuria is a severe form of menta...

Phenylketonuria is a severe form of mental disability caused by a homozygous recessive allele. The condition affects about 1 in 10,000 neborn Caucasians. Estimate the frequency of

Define the purposes of counselling, Q. Define the Purposes of Counselling? ...

Q. Define the Purposes of Counselling? 1. Supporting individuals to take charge of their own life by: Providing information; Facilitating emotional adjustments; and

How are human voices created, How are human voices created? Human voice...

How are human voices created? Human voice is formed with the help of muscles in the neck and the vocal cords. The tighter the vocal cords the higher the pitch of voice. This is

Taxonomy.., what is the androcium in china rose ..

what is the androcium in china rose ..

Intermediate filaments - role of cytoskeleton structures, Intermediate fila...

Intermediate filaments - Role of Cytoskeleton Structures These filaments are intermediate in size among microtubules and microfilaments and are 10 nm in diameter. Five classes

Energy flow in an eco-systems, The capacity to do work is known as energy. ...

The capacity to do work is known as energy. In an ecosystem energy flows through one organism to another in the form of food. The flow of energy is uni-direction and non cycle and

How do derived characters help cladistic taxonomists, How do derived charac...

How do derived characters help cladistic taxonomists determine phylogenetic relationships? A derived character is found only between members of a particular group. Thus, cladi

Growing roots from different parts of plants, Growing roots from different ...

Growing roots from different parts of plants Secure a box of sand and place it away from direct sunlight. Wet the sand thoroughly and keep it moist. Plant the following thi

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd