Define method used for capsular staining - anthony staining, Biology

Assignment Help:

Define method Used for Capsular Staining - Anthony Staining Method?

Another method used for capsular staining is Anthony staining method, devised by E.E. Anthony in 1931. The method involves the use of a primary stain i.e., 1% aqueous crystal violet. Both the capsular material and the cell wall will take the colour of the stain and will appear dark blue. But the capsule being nonionic will not absorb the primary stain. It adheres to the capsule without binding to it. Because capsule is water soluble, therefore, copper sulphate is used both as a decolourizing agent and as a counter stain. It takes out the colour from the capsular material and counter stains the decolourized capsule which now appears as a light blue zone surrounding deep purple cells. In both Klebsiella and Bacillus, capsule can be seen surrounding the dark purple coloured cells. Capsule will appear as a clear zone in negative staining against dark background, whereas, it is light blue coloured in anthony's method.

 


Related Discussions:- Define method used for capsular staining - anthony staining

Advantage and disadvantage of steam sterilization, Q. Advantage and Disadva...

Q. Advantage and Disadvantage of steam sterilization? Advantage: most efficient and reliable sterilization method available. Its quite simple to operate and relatively inexpens

Explain the control of air pollution, Explain the Control of Air Pollution ...

Explain the Control of Air Pollution a. Air belongs to everyone Need it to treat air pollution as public problem   b. Air pollution is an inevitable concomitan

Observation of bumpus, An interesting observation made by an American biolo...

An interesting observation made by an American biologist H.C. Bumpus (1899) provides a good explanation for normalising selection. Bumpus collected some 136 injured house sparrows

What are the two main morphological patterns of cnidarians, What are the tw...

What are the two main morphological patterns of cnidarians? Concerning locomotion how do these forms differentiate from each other? Morphologically, cnidarians categorize as po

Oxidation-reduction potential, How the Oxidation-reduction potential influe...

How the Oxidation-reduction potential influence the spoilage of meat? You learnt earlier that the presence or absence of oxygen and the 0-R potential of the food itself has a b

Elaborate evolution by natural selection , Elaborate Evolution by Natural S...

Elaborate Evolution by Natural Selection ? The conelaboratecept of natural selection lies at the heart of Darwin's Theory of Evolution. Darwin understood that plants and animal

What is genetic recombination and evolution , What is Genetic Recombination...

What is Genetic Recombination and Evolution ? Variation among the offspring of living organisms results from genetic recombination through sexual reproduction and mutation. Va

Conduits-surgery for coronary artery disease, Conduits Figure: Ve...

Conduits Figure: Venous Conduits Reversed saphenous vein was the first conduit used for CABG. 'It is usually harvested from the leg starting above the ankle going up

List five advantages of dsme, Q. List five advantages of DSME? Followin...

Q. List five advantages of DSME? Following are the advantages of DSME. It enables the patient to 1. Accept the disease. 2. Gain knowledge about disease, its prevention

What do you mean by branchial basket, What do you mean by branchial basket?...

What do you mean by branchial basket? In ascidian adults the pharynx is enlarged to form branchial basket. Pharyngeal openings in wall of the basket allow water to pass through

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd