Define method used for capsular staining - anthony staining, Biology

Assignment Help:

Define method Used for Capsular Staining - Anthony Staining Method?

Another method used for capsular staining is Anthony staining method, devised by E.E. Anthony in 1931. The method involves the use of a primary stain i.e., 1% aqueous crystal violet. Both the capsular material and the cell wall will take the colour of the stain and will appear dark blue. But the capsule being nonionic will not absorb the primary stain. It adheres to the capsule without binding to it. Because capsule is water soluble, therefore, copper sulphate is used both as a decolourizing agent and as a counter stain. It takes out the colour from the capsular material and counter stains the decolourized capsule which now appears as a light blue zone surrounding deep purple cells. In both Klebsiella and Bacillus, capsule can be seen surrounding the dark purple coloured cells. Capsule will appear as a clear zone in negative staining against dark background, whereas, it is light blue coloured in anthony's method.

 


Related Discussions:- Define method used for capsular staining - anthony staining

What is relationship cph in photon and frequency of photon, what is relatio...

what is relationship between numbers CPH in photon and frequency of photon? Answer; the photon contains n CPH and its energy is E=hν, when photons energy increases that the num

Define hormonal imbalance as a factor for obesity, Define Hormonal imbalanc...

Define Hormonal imbalance as a factor for obesity? Certain diseases associated with secretion of hormones, e.g., hypothyroidism, hypogonadism and Cushing's syndrome exhibit obe

What is the combination of human advances, Please consider the phenomenon o...

Please consider the phenomenon of bacterial resistance to antibiotics. How did the combination of human advances and basic evolutionary principles lead to this situation? How is it

What happens to the dna molecule when the ends, Now try to pull the strands...

Now try to pull the strands apart at the "origin of replication." What happens to the DNA molecule when the ends are restricted

Basic physical concepts of plant water relation, Basic Physical Concepts of...

Basic Physical Concepts of Plant Water Relation The main idea of this section is to explain the concept of chemical potential of water or water potential and the effect of var

How does epistasis affect proportion of phenotypical form, In the hybridiza...

In the hybridization of 2 genes (4 different alleles, 2 of each pair) how does epistasis affect the proportion of phenotypical forms in the F2 generation? In the dihybridism wi

Eggs found in storage, I stored a few of my things from April to September ...

I stored a few of my things from April to September this year. When I wanted to unpack the boxes, I found hundreds of round, small, black things. They look like they might be snail

Braiding technique for sp removal-endodontics principles, Braiding techniqu...

Braiding technique for SP removal: -    Three small size files inserted alongside the silver point. -    Files twisted together to engage silver point -    Withdraw the f

Define sit and reach flexibility component in the humans, Define Sit and Re...

Define Sit and Reach Flexibility component in the humans? The subject sits on a mat with legs extended straight ahead. Legs should be at right angles to a taped the r box, with

What is the role of heart in human body, What is the role of heart in human...

What is the role of heart in human body? The heart is the main pump that circulates the blood and fluid within the body. The heart, beats at 72 times per minute, and pumps abou

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd