Define method used for capsular staining - anthony staining, Biology

Assignment Help:

Define method Used for Capsular Staining - Anthony Staining Method?

Another method used for capsular staining is Anthony staining method, devised by E.E. Anthony in 1931. The method involves the use of a primary stain i.e., 1% aqueous crystal violet. Both the capsular material and the cell wall will take the colour of the stain and will appear dark blue. But the capsule being nonionic will not absorb the primary stain. It adheres to the capsule without binding to it. Because capsule is water soluble, therefore, copper sulphate is used both as a decolourizing agent and as a counter stain. It takes out the colour from the capsular material and counter stains the decolourized capsule which now appears as a light blue zone surrounding deep purple cells. In both Klebsiella and Bacillus, capsule can be seen surrounding the dark purple coloured cells. Capsule will appear as a clear zone in negative staining against dark background, whereas, it is light blue coloured in anthony's method.

 


Related Discussions:- Define method used for capsular staining - anthony staining

Deficiency diseases-selenium and vitamin e deficiency, Selenium and vitamin...

Selenium and vitamin E deficiency Selenium is an essential micro-mineral required for various physiological functions and for immunity against a variety of diseases. It is an i

Explain digestion system, DIGESTION Digestive enzymes break down food p...

DIGESTION Digestive enzymes break down food particles into smaller units. You will see  that the final breakdown products of protein digestion are single amino acids or small c

What parameter can be calculate, In a twin study of 1000 MZ pairs, 625 pair...

In a twin study of 1000 MZ pairs, 625 pairs were found to have both twins with the same form of a given trait. In a study of 1000 DZ twins, 426 pairs were found to have both twins

Types of overpopulation - people overpopulation, Types of overpopulation - ...

Types of overpopulation - People overpopulation People overpopulation refers to a situation where there are more people than the supplies of food, water and other vital resour

Anti platelet drugs-peri operative problems, Anti Platelet Drugs In t...

Anti Platelet Drugs In the early years of CABG, patients used to be put on aspirin and persantin (Dipyridamole). Persantin is not routinely prescribed now. Aspirin dose can b

What are the complications of chronic dyspepsia, Q. What are the complicati...

Q. What are the complications of chronic dyspepsia? Complications of dyspepsia are listed below: • Wright loss: Since eating most of tell provokes the symptoms, patients res

Phase (different interference) contrast microscopy, Phase (different interf...

Phase (different interference) Contrast microscopy: Living cells are mostly   transparent. For viewing under ordinary light microscope, therefore, live cells must be stained wi

What the two pcr amplified strands, You now want to express the gene in E c...

You now want to express the gene in E coli, but unfortunately it has an intron in it that will not be processed out in bacteria, hence it must be removed. (a)  Design P

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd