Define method used for capsular staining - anthony staining, Biology

Assignment Help:

Define method Used for Capsular Staining - Anthony Staining Method?

Another method used for capsular staining is Anthony staining method, devised by E.E. Anthony in 1931. The method involves the use of a primary stain i.e., 1% aqueous crystal violet. Both the capsular material and the cell wall will take the colour of the stain and will appear dark blue. But the capsule being nonionic will not absorb the primary stain. It adheres to the capsule without binding to it. Because capsule is water soluble, therefore, copper sulphate is used both as a decolourizing agent and as a counter stain. It takes out the colour from the capsular material and counter stains the decolourized capsule which now appears as a light blue zone surrounding deep purple cells. In both Klebsiella and Bacillus, capsule can be seen surrounding the dark purple coloured cells. Capsule will appear as a clear zone in negative staining against dark background, whereas, it is light blue coloured in anthony's method.

 


Related Discussions:- Define method used for capsular staining - anthony staining

How has the position of the stomata changed in some plants, How has the pos...

How has the position of the stomata changed in some plants to prevent excessive water loss by transpiration? In some plants that have leaves that be given too much sunlight the

Osmoregulation in non-chordate metazoans, Osmoregulation in Non-Chordate Me...

Osmoregulation in Non-Chordate Metazoans Previously you have studied that in protozoans, particularly fresh water protozoans; contractile vacuoles play a significant role in t

Drawbacks of lamellar model, Drawbacks of Lamellar model This model ...

Drawbacks of Lamellar model This model does not explain the elasticity of PM. Although this model says that PM is elastic but it also states that head of lipid molecules is

Explain saquinavir, Explain Saquinavir Saquinavir (SQV, Fortovase, Invi...

Explain Saquinavir Saquinavir (SQV, Fortovase, Invirase) - When administered as a single protease inhibitor, a soft-gel preparation (Fortovase) with improved bioavailability an

Can you explain embryonic sac, Q. What is embryonic sac? Which are the cell...

Q. What is embryonic sac? Which are the cells that form the embryonic sac? What are their ploidies? The embryonic sac is female gametophyte of angiosperms. The embryonic sac

Role of nacl in metabolism, ROLE OF NaCl - It forms .9% of blood of ...

ROLE OF NaCl - It forms .9% of blood of mammal. It forms .7% of blood of frog. Bicarbonates of Na+ act as buffer for pH constancy. Involved in active transport of gluc

Female gametophyte, Female Gametophyte The development of a female gam...

Female Gametophyte The development of a female gametophyte is initiated with the enlargement of one of the megaspores (usually the one close to the chalaza in a linear tetrad)

Explain what is molds , Explain what is Molds ? Zygomycota, or molds, a...

Explain what is Molds ? Zygomycota, or molds, are a type of fungus made of filamentous hyphae that grow on decomposing material. The molds actually aid in the decomposition pro

Agar - agar, AGA R - AGAR It is cell wall mucopolysaccharide of some r...

AGA R - AGAR It is cell wall mucopolysaccharide of some red algae (e.g. Gelidium, Gelidiella, Gracillaria). Agar is used as culture medium, thickener, stabilizer and as lax

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd