Define method used for capsular staining - anthony staining, Biology

Assignment Help:

Define method Used for Capsular Staining - Anthony Staining Method?

Another method used for capsular staining is Anthony staining method, devised by E.E. Anthony in 1931. The method involves the use of a primary stain i.e., 1% aqueous crystal violet. Both the capsular material and the cell wall will take the colour of the stain and will appear dark blue. But the capsule being nonionic will not absorb the primary stain. It adheres to the capsule without binding to it. Because capsule is water soluble, therefore, copper sulphate is used both as a decolourizing agent and as a counter stain. It takes out the colour from the capsular material and counter stains the decolourized capsule which now appears as a light blue zone surrounding deep purple cells. In both Klebsiella and Bacillus, capsule can be seen surrounding the dark purple coloured cells. Capsule will appear as a clear zone in negative staining against dark background, whereas, it is light blue coloured in anthony's method.

 


Related Discussions:- Define method used for capsular staining - anthony staining

Define needle selection for suturing, Define Needle selection for Suturing ...

Define Needle selection for Suturing 1. Needle with a reverse cutting edge "the cutting edge is outside the curve" is preferable, because it prevents tearing, 2. Conventiona

Clinical symptoms of diabetes mellitus, Q. Clinical Symptoms of diabetes me...

Q. Clinical Symptoms of diabetes mellitus? In mild cases of diabetes mellitus, no symptoms may be seen. The diagnosis could be incidental during a blood or urine investigation.

Protozoa, locomotion in parameceum

locomotion in parameceum

Define methodology for half and full saturation test, Define Methodology fo...

Define Methodology for Half and Full Saturation Test? 1. Take 5 ml each of starch and dextrin solutions, add 5 ml of saturated solution of ammonium sulfate. Shake thoroughly an

Effects of cardiogenic pulmonary edema, Effects of Cardiogenic Pulmonary Ed...

Effects of Cardiogenic Pulmonary Edema Interference with oxygen transfer in the lungs Depression arterial oxygen tension Sense of suffocation and oppression in the

State about dental implant reconstruction, State about Dental implant recon...

State about Dental implant reconstruction Dental implant reconstruction requires a thorough knowledge and understanding of the various strategically important anatomic landmark

Preliminary screening stage of battery, Preliminary screening stage of batt...

Preliminary screening stage of battery During the preliminary screening stage in the development of the battery, items in the original pool that did not attain satisfactory int

Explain reproduction rates variation in bacterial gene, Antibiotic resistan...

Antibiotic resistance is the result of natural selection mutations, the only source of new genetic information available for bacteria. Rapid reproduction rates variation in bacteri

Show technical aspects of the postero-anterior film, Q. Show technical aspe...

Q. Show technical aspects of the postero-anterior film? 1) Identification: Patient identification and side marker must be present. 2) Centering: The thoracic spinous pro

Is transpiration the only way by which leaves lose water, Is transpiration ...

Is transpiration the only way through which leaves lose water? Plants do not only lose water as vapor, as by transpiration. The leaves also lose liquid water by a phenomenon c

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd