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Define method Used for Capsular Staining - Anthony Staining Method?
Another method used for capsular staining is Anthony staining method, devised by E.E. Anthony in 1931. The method involves the use of a primary stain i.e., 1% aqueous crystal violet. Both the capsular material and the cell wall will take the colour of the stain and will appear dark blue. But the capsule being nonionic will not absorb the primary stain. It adheres to the capsule without binding to it. Because capsule is water soluble, therefore, copper sulphate is used both as a decolourizing agent and as a counter stain. It takes out the colour from the capsular material and counter stains the decolourized capsule which now appears as a light blue zone surrounding deep purple cells. In both Klebsiella and Bacillus, capsule can be seen surrounding the dark purple coloured cells. Capsule will appear as a clear zone in negative staining against dark background, whereas, it is light blue coloured in anthony's method.
Living beings are made of organic and inorganic substances. According to the complexity of their molecules how can each of those substances be classified? Inorganic substances
about of moving blood
Define Patient Education for preventive strategies for food allergy? Patient education, therefore, is critical and should include: Allergen identification (e.g., how to
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In this 1st step, the corresponding aminoacyl-tRNA for the second codon binds to the A site by codon-anticodon interaction. Binding of the aminoacyl-tRNA needs elon
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What is polymorphism
Extruded Starch Cereal products, that are prepared by pumping cereals through a small opening for instance, pasta, Ready-to-eat cereals etc.
Tissue Valves : Tissue valves mounted on a frame with a sewing ring are inserted by the same technique as used in the case of prosthetic valves.
What is Chromosome Mapping in phenotypic trait? Chromosome Mapping : By noting how often a gene segment associated with a specific phenotypic trait crossed over during meios
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