Define method used for capsular staining - anthony staining, Biology

Assignment Help:

Define method Used for Capsular Staining - Anthony Staining Method?

Another method used for capsular staining is Anthony staining method, devised by E.E. Anthony in 1931. The method involves the use of a primary stain i.e., 1% aqueous crystal violet. Both the capsular material and the cell wall will take the colour of the stain and will appear dark blue. But the capsule being nonionic will not absorb the primary stain. It adheres to the capsule without binding to it. Because capsule is water soluble, therefore, copper sulphate is used both as a decolourizing agent and as a counter stain. It takes out the colour from the capsular material and counter stains the decolourized capsule which now appears as a light blue zone surrounding deep purple cells. In both Klebsiella and Bacillus, capsule can be seen surrounding the dark purple coloured cells. Capsule will appear as a clear zone in negative staining against dark background, whereas, it is light blue coloured in anthony's method.

 


Related Discussions:- Define method used for capsular staining - anthony staining

Geotropism - root and shoot morphogenesis, Geotropism - Root and Shoot Morp...

Geotropism - Root and Shoot Morphogenesis Roots always grow towards gravity. The lot threshold of geotropic response requires as little time as one minute Gravity pulls auxin

Procedure for testing vanaspati and rancidity in ghee, Procedure for testin...

Procedure for testing Presence of Vanaspati and Rancidity in Ghee? Carry out the steps enumerated herewith for conducting this exercise. For Vanaspati Checking: 1. Take 5

Which are the organs of the excretory system, Which are the organs of the e...

Which are the organs of the excretory system? The excretory system is produced of kidneys (two), ureters (two), bladder and urethra. The Excretory System - Image Diversity:

What is the other name given to sex chromosomes, What is the other name giv...

What is the other name given to sex chromosomes? What is the function of sex chromosomes? Sex chromosomes are also known as allosomes (the other chromosomes that are not sex ch

Green house effect and global warming, The Term "Green House" effect was fi...

The Term "Green House" effect was first introduced by j. Fourier in 1827. The green house effects are the rise in temperature of earth because certain gases in the atmosphere (CO2,

Spermatogenesis, What are the phases of spermatogenesis and how it occurs ?...

What are the phases of spermatogenesis and how it occurs ?

Describe u - waves, Q. Describe U - Waves? The U-wave is usually uprigh...

Q. Describe U - Waves? The U-wave is usually upright if the T is also upright and is highest at low rates. When the heart rate increases to more than 90, the U-wave is rarely v

Fluid management in diarrhoea, Q. Fluid management in Diarrhoea? Fluid ...

Q. Fluid management in Diarrhoea? Fluid management: The key to diarrhoea management is the early replacement of fluid lost in the stools through intravenous or oral route. Whil

What type of light-sensitive cell is present in this region, a) Which regio...

a) Which region of the retina gives the most accurate interpretation of the image? b) What type of light-sensitive cell is present in this region?   (a) The fove

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd