Define method used for capsular staining - anthony staining, Biology

Assignment Help:

Define method Used for Capsular Staining - Anthony Staining Method?

Another method used for capsular staining is Anthony staining method, devised by E.E. Anthony in 1931. The method involves the use of a primary stain i.e., 1% aqueous crystal violet. Both the capsular material and the cell wall will take the colour of the stain and will appear dark blue. But the capsule being nonionic will not absorb the primary stain. It adheres to the capsule without binding to it. Because capsule is water soluble, therefore, copper sulphate is used both as a decolourizing agent and as a counter stain. It takes out the colour from the capsular material and counter stains the decolourized capsule which now appears as a light blue zone surrounding deep purple cells. In both Klebsiella and Bacillus, capsule can be seen surrounding the dark purple coloured cells. Capsule will appear as a clear zone in negative staining against dark background, whereas, it is light blue coloured in anthony's method.

 


Related Discussions:- Define method used for capsular staining - anthony staining

Define national programmes targeting infants and preschooler, Explain Natio...

Explain National Programmes Targeting Infants and Preschoolers? Infants and preschoolers are the vulnerable sections of the society. To give them due  coverage, some national p

Bovine spongiform encephalopathy, Bovine spongiform encephalopathy The ...

Bovine spongiform encephalopathy The bovine transmissible spongiform encephalopathy (BSE), known as 'mad cow disease'-first noticed in Great Britain in 1986, is similar to scra

Plant, what is the scientific explanation of "touch me not" plant that can ...

what is the scientific explanation of "touch me not" plant that can react due to any touch

Food chain, Food chain is the simplest representation of energy flow in th...

Food chain is the simplest representation of energy flow in the community. At the base is energy stored in the plants, which are eaten by the small organisms, which in turn are ea

What are the three main types of viral hepatitis, Q. What are the three mai...

Q. What are the three main types of viral hepatitis? There are various types of viral hepatitis. The most vital epidemiologically are hepatitis A, hepatitis B and hepatitis C.

Causes of heart failure, It is important to recognise underlying causes and...

It is important to recognise underlying causes and precipitating factors of heart failure for its appropriate management. That would also help in prevention and treatment of heart

Male reproductive system - semen, SEMEN - Sperms and secretion of acces...

SEMEN - Sperms and secretion of accesory glands collectively known as seminal fluid or semen. It is milky, semi-solid in nature having particular smell. pH : 7.35 - 7.5. Spe

Nomenclature, who proposed the trinominal nomenclature

who proposed the trinominal nomenclature

Kingdoms, The organisms that live in hostile environments that cannot suppo...

The organisms that live in hostile environments that cannot support other forms of life are members of what kingdom?

What is mitral stenosis, Q. What is mitral stenosis? Most common cause ...

Q. What is mitral stenosis? Most common cause of mitral stenosis is rheumatic fever. Nearly 30 per cent of patients with rheumatic fever may go on to develop pure mitral valve

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd