Define method used for capsular staining - anthony staining, Biology

Assignment Help:

Define method Used for Capsular Staining - Anthony Staining Method?

Another method used for capsular staining is Anthony staining method, devised by E.E. Anthony in 1931. The method involves the use of a primary stain i.e., 1% aqueous crystal violet. Both the capsular material and the cell wall will take the colour of the stain and will appear dark blue. But the capsule being nonionic will not absorb the primary stain. It adheres to the capsule without binding to it. Because capsule is water soluble, therefore, copper sulphate is used both as a decolourizing agent and as a counter stain. It takes out the colour from the capsular material and counter stains the decolourized capsule which now appears as a light blue zone surrounding deep purple cells. In both Klebsiella and Bacillus, capsule can be seen surrounding the dark purple coloured cells. Capsule will appear as a clear zone in negative staining against dark background, whereas, it is light blue coloured in anthony's method.

 


Related Discussions:- Define method used for capsular staining - anthony staining

Determine the medical history - assessment, Determine the Medical History -...

Determine the Medical History - Assessment  A child's medical history often contains predictors of neuropsychological functioning. Perhaps the most obvious predictor is the pre

Deficiency diseases-iodine deficiency, Iodine  deficiency Iodine is an...

Iodine  deficiency Iodine is an important component of thyroid hormones, which play active role in several physiological process such as control of basal metabolic rate, oxidat

Storage and distribution - sterilization process, Packs, pouches, and casse...

Packs, pouches, and cassettes are sometimes wet after completing the sterilization process. This is a problem usually associated with the use of paper or paper/ plastic wraps in t

Who receive any future pandemic flu vaccine, Since the availability of flu ...

Since the availability of flu vaccine is limited, who should receive any future pandemic flu vaccine?

Theoretical calculation of calorific value of a fuel, The calorific value o...

The calorific value of a fuel can be theoretically determined by using dulong's formula. It is assumed that heat evolved comes from the combustion of carbon, hydrogen, and sulphur

What are the female pronucleus, What are the female pronucleus and the male...

What are the female pronucleus and the male pronucleus? The female pronucleus is the proper haploid nucleus of the egg cell. Male pronucleus is the haploid nucleus of the sperm

Suger diabetes, what normally cause type one sugar diabetes

what normally cause type one sugar diabetes

Explain tertiary and quaternary structure of proteins, Explain Tertiary str...

Explain Tertiary structure and Quaternary structure of proteins Tertiary structure Secondary structure, in turn, folds back and bonds to itself in a three-dimensional mann

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd