Define limitations for standard or total plate counts method, Biology

Assignment Help:

Define Limitations for Standard or Total Plate Counts Method?

This method has certain limitations -

1. Microbial count depends on the type of media, incubation temperature, incubation time, distribution of the organism in the food sample and sampling method used.

2. Fastidious microorganisms may not be able to grow on the agar medium used.

3. Strict anaerobes may be overlooked because incubation conditions favour mesophilic aerobes.

4. Fungi may be underestimated because longer incubation time and lower temperature is required for fungal growth.

5. TPC indicates the size of the population but not the exact number of microbial cell because a colony may result from a clump of cells. Both pour plate and spread plate can be used for plating. Spread plate has advantage over pour plate as heat sensitive psychrophiles can be examined.

Also, colonial features can be used for presumptive identification. However, there may be a problem of spreaders and crowding of colonies especially when the agar is not completely dry which makes counting difficult. Further, microaerophiles grow slowly in spread plate.


Related Discussions:- Define limitations for standard or total plate counts method

Can we use ethylene oxide as chemical sterilant, Q. Can we use Ethylene oxi...

Q. Can we use Ethylene oxide as chemical sterilant? Ethylene oxide: This is considered as the only chemical sterilant while the others are considered merely as disinfectan

Which animals make cutaneous respiration, Q. Which animals make cutaneous r...

Q. Which animals make cutaneous respiration? Adult amphibians and Terrestrial annelids make cutaneous respiration in amphibians there is also pulmonary respiration, the thin sk

Nursing assessment - anomalies of stomach and oesophagus, Nursing Assessmen...

Nursing Assessment  These children present with excessive salivation and droofing of saliva from the mouth, coughing, gagging, even choking and cyanosis at the lime of first f

Explain hepatitis b, Hepatitis B  Vaccination against hepatitis B (Enger...

Hepatitis B  Vaccination against hepatitis B (Engerix-B - GlaxoSmithKline; Recombivax HB - Merck) is now a routine pediatric immunization in the US. It is recommended for previo

Glands associated with eyes, GLANDS - 1 .       LACRYMAL GLANDS - ...

GLANDS - 1 .       LACRYMAL GLANDS - Tear glands numbers are 3. Its secretion is aquous & salty, keeps eye ball wet, give nourishment to cornea. Lysozyme also present

What is the chemical nature of carbohydrates, What is the chemical nature o...

What is the chemical nature of carbohydrates? The name carbohydrate owes its origin to the fact that most substances of this class have empirical formulae suggesting that they

What is tubal pregnancy, Q. What is tubal pregnancy? Many times fecunda...

Q. What is tubal pregnancy? Many times fecundation takes place in the Fallopian tubes in general the newly formed zygote is taken to the uterus where nidation and the embryonic

Zoogly, are protozoans primitive to life .

are protozoans primitive to life .

Proteins - digestion process, Proteins are very essential macromolecules as...

Proteins are very essential macromolecules as they have a range of many important functions like: • Required for building and repair of body tissues (including muscle) • Enzy

Why are vaccines made of the own disease agent, Q. Why are vaccines made of...

Q. Why are vaccines made of the own disease agent or of fragments of it? The goal of vaccines is to artificially induce a specific primary immune response (and the consequent f

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd