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Define Ion exchange chromatography?
In this method a reversible exchange of ions is effected. Usually resins and ionic solutes are the two components involved in ion-exchange. Ion exchange resins are of two types cation and anion exchange resins. The acidic or cation exchange resins contain sulfonic acid groups (SO3H), carboxylic groups (COOH) or phenol groups (OH).
Basic or anion exchange resins contain weakly basic amine groups (NH2) or strongly basic quarternary ammonium groups (NH4)+ OH-. The surfaces of acid resins bind cations such as H, Na+, Ca2+, NH4+. The basic or anion exchange resins carry positive charges on the surface and thus adsorb negative ions.
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Find the electric potential, taking zero at infinity, at the upper right corner (the corner without a charge) of the rectangle in the figure. (Let y = 3.7 cm and x = 6.7 cm.)
Biotechnological applications of enzymes Although enzymes have been used in certain industrial processes for centuries, their precise role or even their identity was not known
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