Define instrument for absorbed radiation - spectrophotometer, Biology

Assignment Help:

Define Instrument for Absorbed Radiation - Spectrophotometer?

In contrast to colorimeter, spectrophotometer is an instrument that is capable of splitting the incident radiation into a spectrum producing a radiation of defined wavelength and subsequently measuring the intensity of that radiation. In place of defined wavelength and subsequently measuring the intensity of that radiation. In place of coloured filters, a diffraction grating or glass prism or quartz prism is fitted, which disperses the white light into a continuous spectrum. Total wavelength range, at which measurements can be made includes the visible spectrum (400-750 nm) and extends on each side into the near ultraviolet (360-400 nm) and near infra-red (750-1000 nm).

It is therefore possible to estimate substances which are more or less colourless in the visible region but which absorb light and are therefore coloured, in the ultraviolet or infrared region. These instruments will be available in the laboratory for you to carry out your experiments. Do use them carefully. You should also be aware of the different components/ parts of these instruments. Though it is not very essential, but this information will help you understand the functioning of these instruments more closely. A brief description about the essential parts of photocolorimeter is also included herewith in this section.


Related Discussions:- Define instrument for absorbed radiation - spectrophotometer

Formation of lactate and its consumption, Formation of  lactate and  its ...

Formation of  lactate and  its consumption Formation of  lactate and  its consumption : If anaerobic conctitions prevail, the reoxidation  of NADH  through the respiratory cha

Open pulmonary valvotonzy, Open Pulmonary Valvotonzy, I nfundibular Rese...

Open Pulmonary Valvotonzy, I nfundibular Resection and Trans Annular Patch :  The pulmonary annulus may be narrow based on pre-operative investigations and on measurement

Implant exposure - implant surgery, Describe Implant exposure ? Sound su...

Describe Implant exposure ? Sound surgical principles to minimize the surgical exposure based on the access required should be employed. A series of surgical approaches to achie

Explain leaf silhouettes, Leaf silhouettes Place a leaf on a sheet of w...

Leaf silhouettes Place a leaf on a sheet of white paper and hold it securely with thumb or finger. Press a piece of natural or artificial sponge next to an ink pad. With short,

Define intentional adulteration - types of adulteration, Define Intentional...

Define Intentional Adulteration - Types of Adulteration? In intentional adulteration, the substance is added, removed or substitute knowingly by the adulterator for the purpose

Determine possible polypeptides, a) The image above is part of a DNA sequen...

a) The image above is part of a DNA sequencing gel. Assuming this is the DNA coding strand and you are reading 5' -> 3' what are ALL the possible polypeptides this sequence cou

Calculate the probability that both are carriers for tsd, The male had a fe...

The male had a female first cousin (on his father side) who died from TSD and the female had a maternal uncle with TSD. Assume this trait is very rare. 1. Calculate the probabil

Ecosystem and environment, How ecosyestem concept is useful to the study of...

How ecosyestem concept is useful to the study of environment?

Explain nutrient and storage proteins, Nutrient and Storage proteins Th...

Nutrient and Storage proteins These proteins store amino acids. Ovalbumin is the protein of egg white, used as an amino acid source for the developing embryo. Casein, the prote

Onion cells, What is the process of mitosis in onion root tips in a lab

What is the process of mitosis in onion root tips in a lab

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd