Define important points while working with autoclave, Biology

Assignment Help:

Define Important Points While Working With Autoclave?

Note: Following points should be kept in mind while working with autoclave.

1. Autoclave should not be packed tightly otherwise steam won't be able to come in contact with every object in the autoclave.

2. The air initially present in autoclave chamber should be removed before closing exhaust valve, otherwise temperature won't reach to 121°C though the pressure would be 15 pounds.

3. For larger sample of liquid, autoclave time should be increased so that centre of the liquid should reach to 121°C.

 4. After autoclaving, steam should be released slowly otherwise liquid media would come out. Besides autoclave, hot air ovens are also used for sterilization. Let us get to know about hot air ovens.

 


Related Discussions:- Define important points while working with autoclave

Explain about the oral cavity and alimental-y tract, Explain about the Oral...

Explain about the Oral cavity and alimental-y tract? Various functional changes and decline in secretary function occur in the digestive tract with aging. These include: Or

What is the prevention of perinatal transmission, Prevention of perinatal t...

Prevention of perinatal transmission  Zidovudine alone, started at 14-34 weeks of gestation and continued in the infant for the first 6 weeks of life, reduced HIV transmission

Determine the major foot problem in diabetic patient, Determine the major f...

Determine the major foot problem in diabetic patient The American Diabetic Association (ADA) has estimated that 50% of the limbs with foot ulcers can be saved if both the heal

Explain the frank furcal perforation, Explain the Frank Furcal Perforation ...

Explain the Frank Furcal Perforation a) Coronal perforation at the pulpal floor of multirooted tooth. b) Due to excessive deepening at the pulpal floor during access perfora

What is the phenomenon known as red tide, What is the phenomenon known as "...

What is the phenomenon known as "red tide"? Which ambiental harms can it cause? Red tide is a phenomenon that happens when dinoflagellates (algae from the pyrrophyte group) pro

What are the phases to atherosclerosis, Q. What are the phases to atheroscl...

Q. What are the phases to atherosclerosis? There are five phases to atherosclerosis: Phase I: Asymptomaticphase, consists of fatly streaks which are non-obstructive, lipi

What is passive transport , The passive transport of molecules across a me...

The passive transport of molecules across a membrane does not require an input of metabolic energy. Rate of the diffusion or transport is proportional to the concentration gradient

Production diseases, Pr o d u c t i o n Diseases The production...

Pr o d u c t i o n Diseases The production diseases, which were previously referred as metabolic diseases, include diseases like parturient paresis (milk fever), downer

Salt glands in birds and reptiles, Salt Glands in Birds and Reptiles M...

Salt Glands in Birds and Reptiles Marine reptiles for example, iguanas, estuarine sea turtles, crocodiles, sea snakes and marine birds like the marine teleosts do not produce

Reproduction, what is the difference between anisogamy and oogamy?

what is the difference between anisogamy and oogamy?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd