Define important points while working with autoclave, Biology

Assignment Help:

Define Important Points While Working With Autoclave?

Note: Following points should be kept in mind while working with autoclave.

1. Autoclave should not be packed tightly otherwise steam won't be able to come in contact with every object in the autoclave.

2. The air initially present in autoclave chamber should be removed before closing exhaust valve, otherwise temperature won't reach to 121°C though the pressure would be 15 pounds.

3. For larger sample of liquid, autoclave time should be increased so that centre of the liquid should reach to 121°C.

 4. After autoclaving, steam should be released slowly otherwise liquid media would come out. Besides autoclave, hot air ovens are also used for sterilization. Let us get to know about hot air ovens.

 


Related Discussions:- Define important points while working with autoclave

Heredity and variations, HEREDITY 1.         Like begets like i.e; ever...

HEREDITY 1.         Like begets like i.e; every living organism reproduces its own kind. Frog reproduce frogs, rats reproduce rats only. 2.         Heredity includes the cha

Induction of hydrolases - responses to infection, Induction of hydrolases -...

Induction of hydrolases - Responses to Infection Plants do respond to attack by pathogens. To restrict their spread the cells synthesise and secrete enzymes to hydrolyse the c

Pericarditis , Pericarditis Pericarditis is a syndrome caused by infl...

Pericarditis Pericarditis is a syndrome caused by inflammation of the pericardium. Causes i) Infections Bacterial : Pneumococci, Staphylococci, Streptococci,

Phosphorus cycle, Phosphorus cycle is sedimentary cycle because it does not...

Phosphorus cycle is sedimentary cycle because it does not enter into atmosphere. It can be found on earth in water, soil and sediments. The phosphorus cycle is the biogeochemical c

Define genetic susceptibility factors for obesity, Define Genetic Susceptib...

Define Genetic Susceptibility Factors for Obesity? Some people inherit a tendency to become fat. Earlier, it was thought that genetic inheritance ranged from 66% to 80% but now

Types of stress caused by freezing, Types of Stress Caused by Freezing ...

Types of Stress Caused by Freezing Thus, freezing can cause the following kinds of stresses Lowering of temperature to suboptimum thus reducing the activity of mo

Addition of glycine to the physiological saline, Addition of glycine to the...

Addition of glycine to the physiological saline A complete motor neuron is removed from a frog and placed in a large volume of normal physiological saline.  The neuron is heal

Define about the concept of sports nutrition, Define about the concept of s...

Define about the concept of sports nutrition? The concept of sports nutrition was evolved fundamentally with integration of different disciplines in order to collectively striv

#title.examples of classes of vertebrates, how to explain an example from e...

how to explain an example from each class of vertebrate

What are the etiological agents of malaria, What are the etiological agents...

What are the etiological agents of malaria? The etiological agents of malaria are protozoans of the genus Plasmodium. There are four dissimilar types of plasmodia that cause ma

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd