Define important points while working with autoclave, Biology

Assignment Help:

Define Important Points While Working With Autoclave?

Note: Following points should be kept in mind while working with autoclave.

1. Autoclave should not be packed tightly otherwise steam won't be able to come in contact with every object in the autoclave.

2. The air initially present in autoclave chamber should be removed before closing exhaust valve, otherwise temperature won't reach to 121°C though the pressure would be 15 pounds.

3. For larger sample of liquid, autoclave time should be increased so that centre of the liquid should reach to 121°C.

 4. After autoclaving, steam should be released slowly otherwise liquid media would come out. Besides autoclave, hot air ovens are also used for sterilization. Let us get to know about hot air ovens.

 


Related Discussions:- Define important points while working with autoclave

Determine the occurrence of vitamin k, Determine the Occurrence of vitamin ...

Determine the Occurrence of vitamin K Vitamin K is found naturally in plants  and vitamin K2 is synthesized by the bacteria in the intestines in humans. In nature, only two nap

Causes of oesophagitis, Q. Causes of oesophagitis? The causes include t...

Q. Causes of oesophagitis? The causes include tissue erosion by hydrocliloric acid (EIC1) and pepsin, with symptoms of substernal burning, cramping, pressure sensation or sever

Explain natural classification, Natural Classification Natural classifi...

Natural Classification Natural classification is based on the natural characters of the taxa. Some consider natural classification a phylogenetic one reflecting the evolutionar

Developments in the area of nutrient-gene interactions, Define Developments...

Define Developments in the Area of Nutrient-Gene Interactions? This unit introduces you to the recent developments in the area of nutrient-gene interactions. We will first revi

Explain about the light sense, Explain about the Light sense You  must ...

Explain about the Light sense You  must be  aware that when you  enter  into a cinema hall, you  are unable  to  see  and  find  out your  seat  initially for  a few minutes; b

Colibacillosis of newborn animals, Colibacillosis of newborn animals T...

Colibacillosis of newborn animals This is the commonest disease entity of newborn farm animals. In calves the disease  occurs in three forms, viz. enteric colibacillosis manif

Omnispective classification, Omnispective Classification This is the ex...

Omnispective Classification This is the extension of the concept of natural classification put forward by Black Welder (1967). The approach seems quite realistic and pragmatic.

Define immediate preoperative period during surgery, Define Immediate Preop...

Define Immediate Preoperative Period during Surgery? Nothing should be given by mouth for at least 8 hours before a general surgery so that the stomach has no leftover food at

Why plant cycle know as alternation of generations, Q. Why is the plant lif...

Q. Why is the plant life cycle known as alternation of generations? The plant life cycle is called as alternation of generations because in this cycle there are two different f

Aetiology, Aetiology:   A List Of The Causes Of A Disease  (Description Of ...

Aetiology:   A List Of The Causes Of A Disease  (Description Of The Characteristic Causes) Factors Which Influence The Onset Of Disease:  Include "Risk Factors", Life-Style, Sex, A

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd