Define important points while working with autoclave, Biology

Assignment Help:

Define Important Points While Working With Autoclave?

Note: Following points should be kept in mind while working with autoclave.

1. Autoclave should not be packed tightly otherwise steam won't be able to come in contact with every object in the autoclave.

2. The air initially present in autoclave chamber should be removed before closing exhaust valve, otherwise temperature won't reach to 121°C though the pressure would be 15 pounds.

3. For larger sample of liquid, autoclave time should be increased so that centre of the liquid should reach to 121°C.

 4. After autoclaving, steam should be released slowly otherwise liquid media would come out. Besides autoclave, hot air ovens are also used for sterilization. Let us get to know about hot air ovens.

 


Related Discussions:- Define important points while working with autoclave

Zoochory - dispersal of seeds, Zoochory - Dispersal of Seeds Some frui...

Zoochory - Dispersal of Seeds Some fruits are eaten by animals and the seeds are passed out with the excreta (endozoochory). Plums, Lantana, grapes, figs and guava are example

Hazard, Hazards Is an event whether natural or man-made that has potential ...

Hazards Is an event whether natural or man-made that has potential for causing injury, loss of life and damage to the property or the environment. It can be grouped into two broad

What are some of the possible treatments, My friend was injured several yea...

My friend was injured several years ago in a car accident and she was diagnosed with a herniated disc. Can you please explain using the correct anatomical terminology what herniati

Explain the regulation of blood glucose concentration, Explain the Regulati...

Explain the Regulation of Blood Glucose Concentration? A number of mechanisms function to maintain blood glucose at remarkably constant level of 70-100 mg/dl under fasting cond

How is the smoothing tendency of cornea functioned, How is the smoothing te...

How is the smoothing tendency of cornea functioned? Smoothing tendency: In health and in disease condition the epitheliurm has a strong tendency to smoothen the underlyin

Glucose-1-phosphate to glycogen, Glucose-1-phosphate to Glycogen The co...

Glucose-1-phosphate to Glycogen The conversion  of glucose-1-phosphate  to glycogen  is  through UDPG and glycogen synthase. We shall learn about  this later in section under g

Evolution of dental implants, The first evidence of implants dates back to ...

The first evidence of implants dates back to 600 A.D. in the Mayan population. There are reports that the ancient Egyptians attempted intra-osseous implantation of animal teeth or

Cell division., sir i''m not understanding cell division rightly. in ca...

sir i''m not understanding cell division rightly. in case of meiosis both paternal and maternal chromosome goes in two cell separately while in case of mitosis they go alongwit

Parthenogenesis, what are the consequences of parthenogenesis in human life...

what are the consequences of parthenogenesis in human life if humans reproduced through parthenogenesis?

What is biotechnology, What is biotechnology? Biotechnology is the appl...

What is biotechnology? Biotechnology is the application of biological knowledge to get new techniques, materials and compounds of pharmaceutical, medical, agrarian, industrial

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd