Define important points while working with autoclave, Biology

Assignment Help:

Define Important Points While Working With Autoclave?

Note: Following points should be kept in mind while working with autoclave.

1. Autoclave should not be packed tightly otherwise steam won't be able to come in contact with every object in the autoclave.

2. The air initially present in autoclave chamber should be removed before closing exhaust valve, otherwise temperature won't reach to 121°C though the pressure would be 15 pounds.

3. For larger sample of liquid, autoclave time should be increased so that centre of the liquid should reach to 121°C.

 4. After autoclaving, steam should be released slowly otherwise liquid media would come out. Besides autoclave, hot air ovens are also used for sterilization. Let us get to know about hot air ovens.

 


Related Discussions:- Define important points while working with autoclave

Phytochrome - floral induction, Phytochrome - Floral Induction Phytoch...

Phytochrome - Floral Induction Phytochrome a pigment that exists in two different inter convertible forms was discovered by Hendricks and Borthwrick et. al. and was chemically

Foods to be avoided during thyphoid, Foods to be avoided are: High fibr...

Foods to be avoided are: High fibre foods like whole grain cereals and  their products (e.g. whole wheat flour, cracked wheat, whole pulses) Raw vegetables and fruits Fri

Chemical reactions which are catalysed by enzymes, Give two examples of che...

Give two examples of chemical reactions which are catalysed by enzymes in the course of brewing.  In the course of brewing, enzymes in the grain catalyse the conversion of star

Mutation rates, Each gene has a characteristic mutation rate. Mutation rate...

Each gene has a characteristic mutation rate. Mutation rates are often described as an average per gene basis. For instance, Drosophila has one detectable mutation per 10,000 loci,

What is the evolutionary importance of pteridophytes, What is the evolution...

What is the evolutionary importance of pteridophytes? As the first tracheophytes, pteridophytes were also the first plants to extensively colonize the terrestrial environment f

How old is the universe, How old is the universe? From the analysis of ...

How old is the universe? From the analysis of data collected by the Hubble telescope the age of the universe is estimated to be about 12 billion years.

Define arthropod by basic morphology - germ layers, Q. Arthropod identity c...

Q. Arthropod identity card. How are arthropods characterized according to examples of representing beings, basic morphology, type of symmetry, germ layers and coelom, digestive sys

Define Types of plants and animal on earth - taxonomy, Define kinds of plan...

Define kinds of plants and animal on earth - Taxonomy? Taxonomic studies have major objective-the learning of the kinds of plants and animal on earth, their names, their distin

Which kind of polarity do water-soluble have, Which kind of polarity do wat...

Which kind of polarity do water-soluble and fat-soluble substances respectively have? Water-soluble substances are polar molecules, i.e., they have electrically charged areas.

What are the types of antigenic agents, Q. What are the types of antigenic ...

Q. What are the types of antigenic agents that may constitute vaccines? Vaccines can be constituted of dead agents of disease, of inactivated toxins, of inactivated agents of d

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd