Define important points while working with autoclave, Biology

Assignment Help:

Define Important Points While Working With Autoclave?

Note: Following points should be kept in mind while working with autoclave.

1. Autoclave should not be packed tightly otherwise steam won't be able to come in contact with every object in the autoclave.

2. The air initially present in autoclave chamber should be removed before closing exhaust valve, otherwise temperature won't reach to 121°C though the pressure would be 15 pounds.

3. For larger sample of liquid, autoclave time should be increased so that centre of the liquid should reach to 121°C.

 4. After autoclaving, steam should be released slowly otherwise liquid media would come out. Besides autoclave, hot air ovens are also used for sterilization. Let us get to know about hot air ovens.

 


Related Discussions:- Define important points while working with autoclave

Phototropic curvature is the result of uneven distribution, Phototropic cur...

Phototropic curvature is the result of uneven distribution of : 1. Gibberellin 2. Phytochrome 3. Cytokinins 4. Auxin Auxin

Agro-industrial byproducts, Agro-industrial byproducts Agro-industrial...

Agro-industrial byproducts Agro-industrial by-products in Southeast Asia are available in plenty, due to the emphasis on crop cultivation. By-products of agro-industries repre

BNA and RNA, Compared to the original sequence what type of mutation occurr...

Compared to the original sequence what type of mutation occurred on the following DNA sequence? Original: TACGAATTCGATAC Mutant: TACASSTTCCATAC

Why is the o-h bond, Why would the O-H bond in C=O-H and the OH bonding wit...

Why would the O-H bond in C=O-H and the OH bonding with a water molecule be considered weak?

Protoplasmic theory, Protoplasmic Theory The living matter which forms ...

Protoplasmic Theory The living matter which forms the bodies of all living being is a special type of highly organized jelly like viscous semi fluid, and named Sarcode by Dujar

Classification of hypertension, Q. Show basic Classification of hypertensio...

Q. Show basic Classification of hypertension? Unlike many other diseases, hypertension develops insidiously (without symptoms). In 90% of hypertension cases, the cause is unkn

Protozoa, mentions chareacteristics and classificaton of protozoa?

mentions chareacteristics and classificaton of protozoa?

Solid fuels, Primary and secondary solid fuels are widely used in domestic ...

Primary and secondary solid fuels are widely used in domestic and industrial purpose. Wood and coal constitute the important primary solid fuels. The important secondary solid fuel

What is biomaterial, What is Biomaterial Biomaterial is defined, in the...

What is Biomaterial Biomaterial is defined, in the broader sense, as any pharmacologically inert material that is capable of interacting with a living organism without causing

What is esophagus called, What is the valve that separates the stomach from...

What is the valve that separates the stomach from the esophagus called? What is its function? The valve that separates the stomach from the esophagus is the cardia. It has the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd