Define important points while working with autoclave, Biology

Assignment Help:

Define Important Points While Working With Autoclave?

Note: Following points should be kept in mind while working with autoclave.

1. Autoclave should not be packed tightly otherwise steam won't be able to come in contact with every object in the autoclave.

2. The air initially present in autoclave chamber should be removed before closing exhaust valve, otherwise temperature won't reach to 121°C though the pressure would be 15 pounds.

3. For larger sample of liquid, autoclave time should be increased so that centre of the liquid should reach to 121°C.

 4. After autoclaving, steam should be released slowly otherwise liquid media would come out. Besides autoclave, hot air ovens are also used for sterilization. Let us get to know about hot air ovens.

 


Related Discussions:- Define important points while working with autoclave

Soils of tropical and temperate forests, Soils of Tropical and Temperate Fo...

Soils of Tropical and Temperate Forests Although soils of tropical forests are generally poor in nutrients they are able to maintain high productivity under natural conditions

What is the structural flat representation of an amino acid, What is the st...

What is the structural flat representation of an amino acid molecule? An amino acid has a central carbon to which a carboxyl group binds on a side and to which a -R (variable r

What concerns should alek''s healthcare team, Nine-year-old Alek is admitte...

Nine-year-old Alek is admitted to the emergency room with a closed fracture of the right femur. Radiography revels that the fracture crosses the distal epiphyseal plate. What conce

Function of adenosine in consciousness, Q. Function of Adenosine in conscio...

Q. Function of Adenosine in consciousness? The adenosine plays a major role in inducing sleep. Injections of adenosine promote sleep and decrease wakefulness. Conversely, adeno

What is an immunotoxin, Immunotoxin is formed by conjugating or combining a...

Immunotoxin is formed by conjugating or combining an antibody with highly toxic agent.

Explain the term light reflex - neuronal pathways, Explain the term Light r...

Explain the term Light reflex - Neuronal pathways The neuronal pathway for light reflex can be afferent and efferent. In the afferent pathway, the pupillary fibres begin in the

What are the gynoecium and the androecium, Q. What are the gynoecium and th...

Q. What are the gynoecium and the androecium? What are the other structures of flowers? Androecium is set of male reproductive structures of flowers it comprehends the stamens

Define technique of paper chromatography, Define Technique of Paper chromat...

Define Technique of Paper chromatography? Paper chromatography is used as 1. Ascending, 2. Descending or 3. Horizontal technique (circular paper chromatography) It is De

What is arsenite inhibitor, What is Arsenite  inhibitor Arsenite inhib...

What is Arsenite  inhibitor Arsenite inhibits synthesis of ATP,  in the conversion of 1,3-bisphosphoglycerate  to 3- phosphoglycerate, by  causing uncoupling of oxidation and

Can you explain antibiotics, Q. Can you explain Antibiotics? The antibi...

Q. Can you explain Antibiotics? The antibiotics are the chemicals produced by microorganisms which are able to destroy or inhibit the growth of other microorganisms. The therap

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd