Define important points while working with autoclave, Biology

Assignment Help:

Define Important Points While Working With Autoclave?

Note: Following points should be kept in mind while working with autoclave.

1. Autoclave should not be packed tightly otherwise steam won't be able to come in contact with every object in the autoclave.

2. The air initially present in autoclave chamber should be removed before closing exhaust valve, otherwise temperature won't reach to 121°C though the pressure would be 15 pounds.

3. For larger sample of liquid, autoclave time should be increased so that centre of the liquid should reach to 121°C.

 4. After autoclaving, steam should be released slowly otherwise liquid media would come out. Besides autoclave, hot air ovens are also used for sterilization. Let us get to know about hot air ovens.

 


Related Discussions:- Define important points while working with autoclave

Sex-limited traits, Sex-Limited Traits Sex Limited Traits are traits ex...

Sex-Limited Traits Sex Limited Traits are traits expressed only in one sex, although the genes controlling thcm arc prcscnt as wcll as transmitted to both thc sexes. Therefore,

Explain the alternaria - fungi, Explain the Alternaria - Fungi? Classif...

Explain the Alternaria - Fungi? Classification Kingdom - Mycetae Division - Amastigomycota Form Class - Deuteromycetes Form Subclass - Hyphomycetidae Form Order

Types of respiratory organs, Types of Respiratory Organs Respiratory o...

Types of Respiratory Organs Respiratory organs may be of the following types: Those that have respiratory surface turned out forming an evagination. These are called gi

Analysis of developmental potency of nuclei, Analysis of Developmental Pote...

Analysis of Developmental Potency of Nuclei The instances given above and many other observations and experimental results of this nature provide tough support to the hypothes

Can we make artificial cells, Q. Can we make artificial cells? Research...

Q. Can we make artificial cells? Research in progress at the "National Aeronautical and Space Administration (NASA)" is focused on artificial cells as a means to deliver medici

When atp gives energy to the cellular metabolism, When ATP gives energy to ...

When ATP gives energy to the cellular metabolism it loses one of its phosphates and ADP reappears. ADP can also lose more phosphates and produce AMP (adenosine monophosphate) o

Placenta, PLACENTA - A common tissue of foetus & mother (uterus) which ...

PLACENTA - A common tissue of foetus & mother (uterus) which is physical, physiological & endocrinal connection is known as placenta. FUNCTIO N - To provide nutrients

Treatment of diarrhoea, Treatment   Most cases of diarrhoea do not need...

Treatment   Most cases of diarrhoea do not need antibiotic therapy as the bacterial or parasitic organism are not isolated from most of the cases. However chemotherapeutics  ar

How is litmus paper, How is litmus paper and testing for acid, base, and ne...

How is litmus paper and testing for acid, base, and neutral, related to biology?

Describe the tailored to the individual patient, Describe the tailored to t...

Describe the tailored to the individual patient? Patent ductus arteriosus (PDA) interruption (surgical or transcatheter intervention): It is a curative procedure. Atrial

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd