Define important points while working with autoclave, Biology

Assignment Help:

Define Important Points While Working With Autoclave?

Note: Following points should be kept in mind while working with autoclave.

1. Autoclave should not be packed tightly otherwise steam won't be able to come in contact with every object in the autoclave.

2. The air initially present in autoclave chamber should be removed before closing exhaust valve, otherwise temperature won't reach to 121°C though the pressure would be 15 pounds.

3. For larger sample of liquid, autoclave time should be increased so that centre of the liquid should reach to 121°C.

 4. After autoclaving, steam should be released slowly otherwise liquid media would come out. Besides autoclave, hot air ovens are also used for sterilization. Let us get to know about hot air ovens.

 


Related Discussions:- Define important points while working with autoclave

How are artificial and natural pearls created, Q. How are artificial and na...

Q. How are artificial and natural pearls created? Pearls are made from small strange particles that deposit between the mantle and the shell of the oyster. These particles trig

Botany, Suggest a mechanism that would allow a plant to grow better if it h...

Suggest a mechanism that would allow a plant to grow better if it had intermorph neighbors than if it had intramorph neighbors. Think about the degree of genetic similarity between

Explain congenital heart disease (chd), Explain Congenital Heart Disease (C...

Explain Congenital Heart Disease (CHD)? Congenital heart disease is an abnormality at birth in cardiac circulatory structure or function. Optimal management of CHD aims not mer

What is risk scoring, What is Risk scoring ? Risk scoring is an instrum...

What is Risk scoring ? Risk scoring is an instrument to assess both the relative risk as well as the absolute risk of developing Cardio-vascular disease and mortality in an ind

Calcium control of glycogen metabolism, As we have seen, in during epinephr...

As we have seen, in during epinephrine hormonal control or glucagon, dephosphorylated   phosphorylase   kinase   is activated by being phosphorylated by protein kinase. This then a

Fungi, a soil surface of 10cm2 may contain what type of hyphae?

a soil surface of 10cm2 may contain what type of hyphae?

How structure of the subunits changed, The holoenzyme is denatured by heat ...

The holoenzyme is denatured by heat at very high temperature. It is found that two polypeptides, although denatured, contains chains that remain together as a unit, while two other

Explain the anabolic phase - dietary management for burns, Explain the Anab...

Explain the Anabolic Phase - Dietary Management for Burns? During this period the patient is well hydrated and the reactions due to metabolic stress are under control. The pati

What are the etiological agents of malaria, What are the etiological agents...

What are the etiological agents of malaria? The etiological agents of malaria are protozoans of the genus Plasmodium. There are four dissimilar types of plasmodia that cause ma

Name the corrosion resistant metals, Name the corrosion resistant metals ...

Name the corrosion resistant metals Most of the corrosion resistant metals or alloys used for implants have a layer of oxides on their surface. The thin (approximately 5 nm) la

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd