Define important points while working with autoclave, Biology

Assignment Help:

Define Important Points While Working With Autoclave?

Note: Following points should be kept in mind while working with autoclave.

1. Autoclave should not be packed tightly otherwise steam won't be able to come in contact with every object in the autoclave.

2. The air initially present in autoclave chamber should be removed before closing exhaust valve, otherwise temperature won't reach to 121°C though the pressure would be 15 pounds.

3. For larger sample of liquid, autoclave time should be increased so that centre of the liquid should reach to 121°C.

 4. After autoclaving, steam should be released slowly otherwise liquid media would come out. Besides autoclave, hot air ovens are also used for sterilization. Let us get to know about hot air ovens.

 


Related Discussions:- Define important points while working with autoclave

Define the normal squid intracellular saline, At 1 AM, a researcher places ...

At 1 AM, a researcher places a healthy squid giant axon in a bath of normal squid physiological extracellular saline and internally perfuses the axon with normal squid intracellula

Explain the steps for injecting insulin injection, Explain the Steps for In...

Explain the Steps for Injecting Insulin Injection It is important to teach patient to take injections at regular times, 30 to 60 minutes before meals. Take insulin everyday, ev

Availability of adp, Availability of ADP When  the ADP  levels increase...

Availability of ADP When  the ADP  levels increase due to hydrolysis ofATP  in various biosynthetic reactions,  the  rate of reaction to generate ATP  is accelerated and this i

Define the effect of deficiency of vitamin c, Define the effect of Deficien...

Define the effect of Deficiency of Vitamin C? Severe ascorbic acid deficiency results in the development of the disease known as scurvy, three important manifestations of scur

Are the alleles of a gene essentially originated, Are the alleles of a gene...

Are the alleles of a gene essentially originated one from the father and the other from the mother? Are there exceptions? It is natural so that alleles have come one from the m

Porth''s pathophysiology, Which of the following patients will likely exper...

Which of the following patients will likely experience difficulty in maintaining lipoprotein synthesis resulting in elevated LDL levels? A physician is explaining to a group of med

Define causes of iron deficiency anaemia, Define Causes of iron Deficiency ...

Define Causes of iron Deficiency Anaemia? Anaemia is a condition in which the blood cannot carry enough oxygen. This may be because there are fewer red blood cells than normal,

Define objectives to know about methods of food processing, Define Objectiv...

Define Objectives to know about methods of food processing? After studying this unit, you will be able to: Enumerate the different methods of food processing Enlist

What is the function of the antidiuretic hormone, Q. What is the function o...

Q. What is the function of the antidiuretic hormone? Where it made and which is the stimuli that increase or reduce its secretion? The antidiuretic hormone is secreted by the h

Describe paradoxical and reverse split s in second heart, Describe Paradoxi...

Describe Paradoxical and Reverse Split S in second heart sound? Sequence of seminar valve closure reversed i.e. P, proceeds A. A reversed split is nearly always due to delayed

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd