Define important points while working with autoclave, Biology

Assignment Help:

Define Important Points While Working With Autoclave?

Note: Following points should be kept in mind while working with autoclave.

1. Autoclave should not be packed tightly otherwise steam won't be able to come in contact with every object in the autoclave.

2. The air initially present in autoclave chamber should be removed before closing exhaust valve, otherwise temperature won't reach to 121°C though the pressure would be 15 pounds.

3. For larger sample of liquid, autoclave time should be increased so that centre of the liquid should reach to 121°C.

 4. After autoclaving, steam should be released slowly otherwise liquid media would come out. Besides autoclave, hot air ovens are also used for sterilization. Let us get to know about hot air ovens.

 


Related Discussions:- Define important points while working with autoclave

Fission - asexual reproduction, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

What are biotic factors, What are biotic factors? Biotic factors are li...

What are biotic factors? Biotic factors are living beings (plants, microorganisms and animals) that are part of a given environment.

Define other consideration for dietary management, Define other considerati...

Define other consideration for Nutrient Requirement and Dietary Management Oral intake is generally feasible only during the anabolic phase wherein the patient should be given

What is benedict's test and its principle, What is Benedict's Test and its ...

What is Benedict's Test and its Principle? This test is answered by all reducing sugars with a free aldehyde or ketone groups. Monosaccharides possess a free aldehyde or ketone

Explain insect resistance, In 2003 nearly 70 million hectares globally were...

In 2003 nearly 70 million hectares globally were planted in genetically modi?ed crops. Of these, 73%  were plants such as soybean, corn and cotton, which were modi?ed to be he

Determine the synaptic process, Since neurotransmitters are not consumed in...

Since neurotransmitters are not consumed in the synaptic process, what are the mechanisms to decrease their concentrations in the synaptic cleft after they have been used? As t

Studying life histories of insects, Studying life histories of insects ...

Studying life histories of insects Cut large rectangular holes in the sides of a big cardboard container and cover them with muslin folded over at the edges and gummed or paste

Explain about the spectrophotometer, Explain about the Spectrophotometer? ...

Explain about the Spectrophotometer? The spectrophotometer, a key instrument today in biomedical laboratories, was invented in 1939 by the American chemist Arnold O. Beckman (1

Living beings are made of inorganic and organic substances, Q. Living being...

Q. Living beings are made of inorganic and organic substances. According to the molecular complexity how can each of those substances be classified? Inorganic substances, mole

Maedi/visna, Maedi/visna Maedi, a progressive pneumonia, and visna, a n...

Maedi/visna Maedi, a progressive pneumonia, and visna, a neurotropic disease, found in sheep and goats are caused by the Maedi/visna virus, belonging to the family Retroviridae

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd