Define important points while working with autoclave, Biology

Assignment Help:

Define Important Points While Working With Autoclave?

Note: Following points should be kept in mind while working with autoclave.

1. Autoclave should not be packed tightly otherwise steam won't be able to come in contact with every object in the autoclave.

2. The air initially present in autoclave chamber should be removed before closing exhaust valve, otherwise temperature won't reach to 121°C though the pressure would be 15 pounds.

3. For larger sample of liquid, autoclave time should be increased so that centre of the liquid should reach to 121°C.

 4. After autoclaving, steam should be released slowly otherwise liquid media would come out. Besides autoclave, hot air ovens are also used for sterilization. Let us get to know about hot air ovens.

 


Related Discussions:- Define important points while working with autoclave

ANIMAL TRANSPORT SYSTEM, WWHAT ARE THE ORGANS THAT ARE INVOLVDED IN THE TRA...

WWHAT ARE THE ORGANS THAT ARE INVOLVDED IN THE TRANSPORTTATION SYSTEM OF ANIMALS

The deficiency in hepatic glucose 6 phosphatase, Under fasting conditions, ...

Under fasting conditions, individuals with a deficiency in hepatic glucose 6 phosphatase cannot dephosphorylate glucose 6 phosphate generated from liver glycogenolysis. As a result

Structure of the vascular cambium, Structure of the Vascular Cambium T...

Structure of the Vascular Cambium This meristem, in the anatomical sense of the term usually includes two histologically distinct kinds of cells: i) Fusiform Initials, i

Skeletal tissues - cartilage, SKELETA L TISSUES - These connective ...

SKELETA L TISSUES - These connective tissues form the endoskeleton of the vertebrates. These support the body, protect the various organs and help in locomotion. Skel

Show the characteristics of honey, Honey comes under the purview of Prevent...

Honey comes under the purview of Prevention of Food Adulteration Act (PFA). Due to its limited production and high cost, honey is prone to adulteration by cane sugar, invert sy

Flies are expected to have vestigial wings and ebony bodies, In the fruit f...

In the fruit fly Drosophila, a rudimentary wing called "vestigial" and dark body color called "ebony" are inherited at independent loci and are recessive to their dominant wild-typ

Evolution, species dynamicsresemble what

species dynamicsresemble what

Fire protection - factors affecting occupational health, Fire Protection - ...

Fire Protection - Factors Affecting Occupational Health Fire protection and extinguishing devices require to be installed at proper places in the work place and proper notices

Define nutritional requirements of fats and oils for adults, Define Nutriti...

Define Nutritional Requirements of Fats and Oils for Adults? A desirable amount of a linoleic acid to be consumed by a normal adult is 3 en% (ICMR, 1990). The invisible fat pre

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd