Define important points while working with autoclave, Biology

Assignment Help:

Define Important Points While Working With Autoclave?

Note: Following points should be kept in mind while working with autoclave.

1. Autoclave should not be packed tightly otherwise steam won't be able to come in contact with every object in the autoclave.

2. The air initially present in autoclave chamber should be removed before closing exhaust valve, otherwise temperature won't reach to 121°C though the pressure would be 15 pounds.

3. For larger sample of liquid, autoclave time should be increased so that centre of the liquid should reach to 121°C.

 4. After autoclaving, steam should be released slowly otherwise liquid media would come out. Besides autoclave, hot air ovens are also used for sterilization. Let us get to know about hot air ovens.

 


Related Discussions:- Define important points while working with autoclave

What is linkage, What is linkage? Two genes are assumed to be under lin...

What is linkage? Two genes are assumed to be under linkage, or linked, when they reside in the same chromosome. For instance, the research of the human genome discovered tha

Define reaction of fehling soxhlet method, Define Reaction of Fehling Soxhl...

Define Reaction of Fehling Soxhlet method? The reducing sugars formed are acted upon by the alkali of Fehling's solution to form enediols. These enediols are very unstable and

Butter, Ask question #Minimum 100 words role of micro organisms in preparat...

Ask question #Minimum 100 words role of micro organisms in preparation of butter accepted#

What is demographic transition, What is Demographic Transition? The who...

What is Demographic Transition? The whole process of change with regard to population size and characteristics is called demographic transition. In order to understand this ter

List the exact sizes of the fragments of digests, (1)  The 7,893 bp plasmid...

(1)  The 7,893 bp plasmid shown below was digested with a series of restriction enzymes (P = PstI, B = BamHI, S = SalI, X = XbaI). Answer the questions below.  a) Indicate

Functions of testis, Functions of Testis The testis performs the follo...

Functions of Testis The testis performs the following two functions: Proliferation of spermatozoa and Secretion of steroid hormones

Respiration, what is known as respiration in animals?

what is known as respiration in animals?

Explain environmental resistance and the population growth, What is the rel...

What is the relationship among environmental resistance and the population growth according to the biotic potential curve and the real population growth curve? The difference

Homeostasis, negative and positive feedback

negative and positive feedback

Ecology, does jellyfish depend on solarenergy

does jellyfish depend on solarenergy

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd