Define important points while working with autoclave, Biology

Assignment Help:

Define Important Points While Working With Autoclave?

Note: Following points should be kept in mind while working with autoclave.

1. Autoclave should not be packed tightly otherwise steam won't be able to come in contact with every object in the autoclave.

2. The air initially present in autoclave chamber should be removed before closing exhaust valve, otherwise temperature won't reach to 121°C though the pressure would be 15 pounds.

3. For larger sample of liquid, autoclave time should be increased so that centre of the liquid should reach to 121°C.

 4. After autoclaving, steam should be released slowly otherwise liquid media would come out. Besides autoclave, hot air ovens are also used for sterilization. Let us get to know about hot air ovens.

 


Related Discussions:- Define important points while working with autoclave

Wbcs types, mode of action of wbcs types

mode of action of wbcs types

What are flagella and cilia, Q. What are flagella and cilia and how do thes...

Q. What are flagella and cilia and how do these structures acquire movement? What are some examples of flagellated and ciliated cells in humans? Flagella and Cilia are structur

How vitamin k used to prevents bone loss, How Vitamin K used to Prevents bo...

How Vitamin K used to Prevents bone loss? Vitamin K is known to inhibit bone loss through inhibiting effect on osteoclast formation. Thus, adequate levels of vitamin K must be

Objection of cell theory, OBJECTION OF CELL THEORY (REASONS OF DISCARTION) ...

OBJECTION OF CELL THEORY (REASONS OF DISCARTION) (i)       Living organism are composed of various types of cells and their product but members of monera & protista don't have

Explain what is enzymes, Explain what is Enzymes? Enzymes are organic...

Explain what is Enzymes? Enzymes are organic substances that speed up, or catalyze, a chemical reaction. At a given temperature, molecules have varying amounts of energy, and

Annuloplasty with a ring, Annuloplasty with a Ring :  The lings commo...

Annuloplasty with a Ring :  The lings commonly used are Carpentier- Edwards and Cosgrove's flexible half ring. The rings are C shaped and have incomplete circles to accommoda

How successful are you at synthesizing genes, How successful are you at syn...

How successful are you at synthesizing genes with really high G/C content? A: We have an extremely low failure rate, which is about one out of five thousand or ten thousand gen

Explain catastrophism, Catastrophism Once-popular belief which events in t...

Catastrophism Once-popular belief which events in the history of earth had occurred in the past a sudden events and by processes different from those operating today. The periods

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd