Define important points while working with autoclave, Biology

Assignment Help:

Define Important Points While Working With Autoclave?

Note: Following points should be kept in mind while working with autoclave.

1. Autoclave should not be packed tightly otherwise steam won't be able to come in contact with every object in the autoclave.

2. The air initially present in autoclave chamber should be removed before closing exhaust valve, otherwise temperature won't reach to 121°C though the pressure would be 15 pounds.

3. For larger sample of liquid, autoclave time should be increased so that centre of the liquid should reach to 121°C.

 4. After autoclaving, steam should be released slowly otherwise liquid media would come out. Besides autoclave, hot air ovens are also used for sterilization. Let us get to know about hot air ovens.

 


Related Discussions:- Define important points while working with autoclave

Determine the groups of soil organisms, Determine the groups of soil organi...

Determine the groups of soil organisms The soil organisms are classified into two broad groups viz. soil flora (related to plants) and soil fauna (related to animals) which ar

Counselling technique of diabetic patient, Counselling a diabetic patient a...

Counselling a diabetic patient and their family members is very important as patient has to modify the life style and diet and needs family support. Therefore it is important for y

Female reproductive system, FEMALE REPRODUCTIVE SYSTEM - In female mamm...

FEMALE REPRODUCTIVE SYSTEM - In female mammals the female reproductive system characteristically comprises of the ovary, uterus, placenta, vagina and vulva.

Estimation of protein by growth and body, Define Estimation of Protein by G...

Define Estimation of Protein by Growth and body, weight changes? The methods under this category include Protein efficiency ratio (PER) Net protein ratio (NPR)

Genetic, 1. The genes for ruby eyes (rb), tan body (t) and cut wings (ct) a...

1. The genes for ruby eyes (rb), tan body (t) and cut wings (ct) are all found on the X-chromosome of Drosophila melanogaster. All of these are recessive traits. They map in the or

What do you mean by oxalates, Q. What do you mean by Oxalates? Oxalates...

Q. What do you mean by Oxalates? Oxalates are widely distributed in plant foods mostly in the form of calcium salts. Oxalates are known to interfere with calcium absorpti

Describe about the mitochondrial membranes, Describe about the mitochondria...

Describe about the mitochondrial membranes The composition of the two mitochondrial membranes is entirely different. The outer membrane is rich in lipids and contains nearly 50

Explain phylum myxornycetes, Phylum Myxornycetes 1) They are known as c...

Phylum Myxornycetes 1) They are known as cellular slime moulds mostly grow in damp places e.g. soil and rotting tree trunks. 2) They have a curious life-cycle in which free

What is pulmonary veins in normal pulmonary vasculature, Q. What is Pulmona...

Q. What is Pulmonary Veins in Normal Pulmonary Vasculature? The right and left upper lobe or superior pulmonary veins descend lateral to the arteries, cross in front of the hil

What is fermentation, What is Fermentation ? Fermentation is a process ...

What is Fermentation ? Fermentation is a process used by anaerobic organisms and certain cells of aerobic organisms, such as muscle cells deprived of oxygen, to obtain addition

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd