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Define Important Points While Working With Autoclave?
Note: Following points should be kept in mind while working with autoclave.
1. Autoclave should not be packed tightly otherwise steam won't be able to come in contact with every object in the autoclave.
2. The air initially present in autoclave chamber should be removed before closing exhaust valve, otherwise temperature won't reach to 121°C though the pressure would be 15 pounds.
3. For larger sample of liquid, autoclave time should be increased so that centre of the liquid should reach to 121°C.
4. After autoclaving, steam should be released slowly otherwise liquid media would come out. Besides autoclave, hot air ovens are also used for sterilization. Let us get to know about hot air ovens.
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Precautions for Preparation of Selective and Differential Medium 1. Dissolve ingredients one by one. 2. Check and set the medium to appropriate pH. 3. Autoclave the mediu
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