Define important points while working with autoclave, Biology

Assignment Help:

Define Important Points While Working With Autoclave?

Note: Following points should be kept in mind while working with autoclave.

1. Autoclave should not be packed tightly otherwise steam won't be able to come in contact with every object in the autoclave.

2. The air initially present in autoclave chamber should be removed before closing exhaust valve, otherwise temperature won't reach to 121°C though the pressure would be 15 pounds.

3. For larger sample of liquid, autoclave time should be increased so that centre of the liquid should reach to 121°C.

 4. After autoclaving, steam should be released slowly otherwise liquid media would come out. Besides autoclave, hot air ovens are also used for sterilization. Let us get to know about hot air ovens.

 


Related Discussions:- Define important points while working with autoclave

Limitations of ecological pyramids, Limitations of Ecological Pyramids ...

Limitations of Ecological Pyramids The pyramid of energy is a significant improvement over the previous two types of ecological pyramids, yet all of them overlook one or anot

Explain the periapical surgery - endodontic surgery, Explain the Periapical...

Explain the Periapical surgery - Endodontic Surgery a) Curretage 1 b) Root-end ressection 2 c) Root-end preparation 3 d) Root-end filling 4

Calculate the frequencies of heterozygotes, If dimples may be considered a ...

If dimples may be considered a single-gene dominant trait, and 64% of the population features dimples, calculate the frequencies of heterozygotes and both types of homozygotes, ass

How many of each type of core histone, How many of each type of core histon...

How many of each type of core histone would it take to wrap the entire human genome into nucleosomes? How has evolution solved the problem of producing such a large number of prote

Speciation, Ernst Mayr, for whom, species and species problem have been the...

Ernst Mayr, for whom, species and species problem have been the main concern of research, points out that speciation, the multiplication of species, that is division of one parent

Depth and currents, Depth and Currents Depth The sea is v...

Depth and Currents Depth The sea is very deep varying in different regions. Generally life extends to all depths but is confined more to the continental shelf and

Protein, What''s the classification of simple protein?

What''s the classification of simple protein?

Ganglia - organisation of nervous system, Ganglia - Organisation of Nervous...

Ganglia - Organisation of Nervous System In between higher non-chordates with a central nervous system, you will observe that the association neurons and motor neurons are con

Define mechanism for copper absorption, Define Mechanism for Copper Absorpt...

Define Mechanism for Copper Absorption? As in the case of other minerals, copper absorption appears to occur by two mechanisms: i) Saturable active mechanism, which operates

Explain total parenteral nutrition, Explain Total Parenteral Nutrition OPN)...

Explain Total Parenteral Nutrition OPN) It is a method of providing, complete nutritional support in which  the gastrointestinal (GI) tract is bypassed by introducing assimilab

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd