Define important points while working with autoclave, Biology

Assignment Help:

Define Important Points While Working With Autoclave?

Note: Following points should be kept in mind while working with autoclave.

1. Autoclave should not be packed tightly otherwise steam won't be able to come in contact with every object in the autoclave.

2. The air initially present in autoclave chamber should be removed before closing exhaust valve, otherwise temperature won't reach to 121°C though the pressure would be 15 pounds.

3. For larger sample of liquid, autoclave time should be increased so that centre of the liquid should reach to 121°C.

 4. After autoclaving, steam should be released slowly otherwise liquid media would come out. Besides autoclave, hot air ovens are also used for sterilization. Let us get to know about hot air ovens.

 


Related Discussions:- Define important points while working with autoclave

How to load glycogen, How to load glycogen? Over the past fifty years, ...

How to load glycogen? Over the past fifty years, the biggest breakthrough was the discovery of how to load glycogen and sophistication in the methods of glycogen loading. Nitro

Why are the salt and sugar used, Q. Why are the salt and sugar used in the ...

Q. Why are the salt and sugar used in the production of dried fruits and dried meat? Substances that sustain a highly hypertonic environment, like salt and sugar, are used in t

Describe the significance of micronucleus., Describe the significance of mi...

Describe the significance of micronucleus. One of two types of dimorphic nuclei found in ciliate protozoans. The single micronucleus contains only one copy of the genome and is

Amino acid metabolism, All tissues have some capability for synthesis of th...

All tissues have some capability for synthesis of the non-essential amino acid remodeling, amino acids and conversion of non-amino acid carbon skeletons into amino acids and other

Processing methods for amelioration of anti-nutritional , Processing Method...

Processing Methods for Amelioration of Anti-Nutritional Meals from many oil seeds often contain undesirable toxic and allergenic compounds that can reduce their nutritive valu

How many alleles of genes that condition x-linked traits do, How many allel...

How many alleles of genes that condition X-linked traits do female and male individuals respectively present? For every correspondent gene to an X- linked trait women present a

Explain endocarditis, Explain Endocarditis The risk of endocarditis is ...

Explain Endocarditis The risk of endocarditis is considered high in patients with last bacterial endocarditis, prosthetic heart valves, complex cyanotic congenital heart diseas

Describe a method of diagnosing diabetes mellitus, Q. In ancient Greece the...

Q. In ancient Greece the father of Medicine, Hypocrates, described a method of diagnosing diabetes mellitus by tasting the patient's urine. What is the physiological explanation fo

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd