Define important points while working with autoclave, Biology

Assignment Help:

Define Important Points While Working With Autoclave?

Note: Following points should be kept in mind while working with autoclave.

1. Autoclave should not be packed tightly otherwise steam won't be able to come in contact with every object in the autoclave.

2. The air initially present in autoclave chamber should be removed before closing exhaust valve, otherwise temperature won't reach to 121°C though the pressure would be 15 pounds.

3. For larger sample of liquid, autoclave time should be increased so that centre of the liquid should reach to 121°C.

 4. After autoclaving, steam should be released slowly otherwise liquid media would come out. Besides autoclave, hot air ovens are also used for sterilization. Let us get to know about hot air ovens.

 


Related Discussions:- Define important points while working with autoclave

Growth rate in animals, GROWTH RATE IN ANIMALS - Growth rate is differe...

GROWTH RATE IN ANIMALS - Growth rate is different at different periods of life. Growth period in human may be studied in 5 steps - (i) Prenatal - In gestation period fo

Telophase of karyokinesis, - The chromosomes at each pole uncoil and elonga...

- The chromosomes at each pole uncoil and elongate to form the chromatin. - A nucleolus reappears at each pole. - Spindle fibers and asters disappear and centrioles split. - A nu

List some differences between a bacteria and a virus, Question 1: (a) L...

Question 1: (a) List some differences between a bacteria and a virus? (b) Define‘Swine Flu'. List the main types of ‘Swine Flu' viruses? (c) Describe the preventive measures

Microbodies, MICROBODIES Single unit membrane bound small spherical str...

MICROBODIES Single unit membrane bound small spherical structures. Microbodies are structurally related to lysosome. On the basis of functions microbodies are of four typ

Explain the term of monosaccharides, Explain the term of Monosaccharides? ...

Explain the term of Monosaccharides? Monosaccharides :  The simplest carbohydrates are Monosaccharides, simple sugars. These generally have three to six carbon atoms, and con

Subdivisions of ecology, Subdivisions of Ecology Ecology was earlier di...

Subdivisions of Ecology Ecology was earlier divided into plant and animal ecology. However, modem ecology does not make any such distinction since plants and animals are intima

Can you explain puffeelfish poisoning, Q. Can you explain Puffeelfish poiso...

Q. Can you explain Puffeelfish poisoning? Tetrodotoxin (TTX) causes pufferfish (tetrodon) poisoning, also known as blowfish poisoning or fugu. How does a pufferfish look l

Dopamine for working memory, Dopamine is important for working memory and d...

Dopamine is important for working memory and drug that increases the level of dopamine in the brain or facilities the action of dopamine, enhances working memory capabilities.Dopam

Explain trypsin, Trypsin Trypsin is secreted in the inactive  form  try...

Trypsin Trypsin is secreted in the inactive  form  trypsinor  -  which is converted into the active form  trypsin by  the enzyme enterokinase secrated by  the duodenal mucosa.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd