Define important points while working with autoclave, Biology

Assignment Help:

Define Important Points While Working With Autoclave?

Note: Following points should be kept in mind while working with autoclave.

1. Autoclave should not be packed tightly otherwise steam won't be able to come in contact with every object in the autoclave.

2. The air initially present in autoclave chamber should be removed before closing exhaust valve, otherwise temperature won't reach to 121°C though the pressure would be 15 pounds.

3. For larger sample of liquid, autoclave time should be increased so that centre of the liquid should reach to 121°C.

 4. After autoclaving, steam should be released slowly otherwise liquid media would come out. Besides autoclave, hot air ovens are also used for sterilization. Let us get to know about hot air ovens.

 


Related Discussions:- Define important points while working with autoclave

Define sample titration for estimation of reducing sugar, Define Sample Tit...

Define Sample Titration for Fehling Soxhlet Method Dilute the given sample glucose solution in 100 ml volumetric flask to the mark with distilled water. Pipette 5 ml of Fehling

Explain normal nutrition - a base of therapeutic diet, Normal nutrition: a ...

Normal nutrition: a base of therapeutic diet Normal nutrition is the foundation upon which the  therapeutic modifications are based. The primary principle of diet nutrition the

What is biogenetic law and define bilateral symmetry, What is Biogenetic La...

What is Biogenetic Law and define Bilateral symmetry? In brief, ontogeny recapitulates phylogeny. Or, developmental or embryological sequence of an animal repeats the evolution

Population distribution, Population Distribution - Population Parameters an...

Population Distribution - Population Parameters and Regulation Dispersion or distribution refers to the pattern of distribution of individuals of a population. As shown in Fig

Hyperlipidemia, Dyslipidemia is an important correctable factor for Coronar...

Dyslipidemia is an important correctable factor for Coronary Artery Disease. There is a strong, independent, continuous, and graded relation between total cholesterol (TC) or low-

What is biotin, Biotin Biotin forms long, colourless needles or a white...

Biotin Biotin forms long, colourless needles or a white crystalline powder. It is sparingly soluble in cold water, but more soluble in hot water and dilute alkalis. Biotin is s

Determine about benefits of jogging, Determine about benefits of Jogging ...

Determine about benefits of Jogging Jogging gives a sense of wellness to a diabetic patient. The frequency of jogging should be at least 3 days in week with a distance of 2 km

How buffer solution can be prepared, How Buffer Solution Can Be Prepared? ...

How Buffer Solution Can Be Prepared? A buffer solution can be prepared to have a desired value of pH by controlling the amount of acid and its salt in case of acidic buffer and

Physical environment and genetics, Physical Environment and Genetic Phy...

Physical Environment and Genetic Physical Environment : Safe water and clean air, healthy workplace, safe houses, communication and roads all contribute to good health. The

Define the spray drying method, Define the Spray Drying Method? Spray d...

Define the Spray Drying Method? Spray drying is a unique drying process since it involves both particle formation and drying. It is most suitable for drying of liquid foods suc

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd