Define important points while working with autoclave, Biology

Assignment Help:

Define Important Points While Working With Autoclave?

Note: Following points should be kept in mind while working with autoclave.

1. Autoclave should not be packed tightly otherwise steam won't be able to come in contact with every object in the autoclave.

2. The air initially present in autoclave chamber should be removed before closing exhaust valve, otherwise temperature won't reach to 121°C though the pressure would be 15 pounds.

3. For larger sample of liquid, autoclave time should be increased so that centre of the liquid should reach to 121°C.

 4. After autoclaving, steam should be released slowly otherwise liquid media would come out. Besides autoclave, hot air ovens are also used for sterilization. Let us get to know about hot air ovens.

 


Related Discussions:- Define important points while working with autoclave

Explain 1st type of hypersensitivity, It is IgE mediated hypersensitivity. ...

It is IgE mediated hypersensitivity. Typical manifestations contain asthma, food allergies, eczema, hay fever etc.

How is the mesoderm of triploblastic animals formed, Q. How is the mesoderm...

Q. How is the mesoderm (third germ layer) of triploblastic animals formed? The mesoderm shows from differentiation of endodermal cells that cover the dorsal region of the arche

Explain beginning and the end of the third interphase period, Q. What are t...

Q. What are the events that mark the beginning and the end of the third interphase period? What happens in the cell in this period? The third interphase period is the G2, It be

Mycoplasmosis-contagious caprine pleuropneumonia (ccpp), Contagious caprine...

Contagious caprine pleuropneumonia (CCPP) Mycoplasma mycoides subsp. capri (Mmc) is the main causative agent of caprine pleuropneumonia, a serious malady of goats in our countr

Pathogens, Give a brief overview of "HIV & Streptococcus Pyogenes" includin...

Give a brief overview of "HIV & Streptococcus Pyogenes" including signs and symptoms. Who is typically affected by this infection?(Children? Immunocompromised patients?)? What is t

Viruses, ae there non-parasitic viruses?

ae there non-parasitic viruses?

How is the extracorporeal digestion, Q. How is the extracorporeal digestion...

Q. How is the extracorporeal digestion associated to predation in arachnids? Arachnids can inoculate poison to kill or paralyze their preys using structures called chelicerae.

How the allele frequencies in a population, A dominant allele will always i...

A dominant allele will always increase in frequency over time. Yes No Q44. When a gene has two alleles, the allele frequencies in a population automatically move toward an equilibr

What are the characteristics of thick gingiva, Characteristics of Thick Gin...

Characteristics of Thick Gingiva  Relatively flat soft tissue and bony architecture  Dense fibrotic soft tissue  Relatively large amount of attached gingiva  Thick und

Watson - crick model how many polynucleotide chains, Q. As per the Watson -...

Q. As per the Watson - Crick Model how many polynucleotide chains does a DNA molecule have? The DNA molecule is formed by two polynucleotide chains bound in antiparallel mode (

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd