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Hexokinase, that catalyzes the first irreversible step of glycolysis, is inhibited by glucose 6-phosphate. Thus when PFK is inhibited and fructose 6-phosphate builds up and so does glucose 6-phosphate due to these two metabolites are in equilibrium by phosphoglucoisomerase. Hexokinase inhibition then reinforces the inhibition at the PFK step. At first sight this seems commonly as it is usually the first irreversible step of a pathway (the committed step) that is the major control step. On this basis, it may appear in which hexokinase should be the main control enzyme not PFK. Furthermore, glucose 6-phosphate the product of the hexokinase reaction can also feed into glycogen synthesis or the pentose phosphate pathway. Thus the first irreversible step which is exclusive to glycolysis is which catalyzed by PFK and therefore this is the main control step.
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i want to extract my compound of interest from tlc i m finding some enzyme inhbitor from microbial source can you plzz suggest me how to prepare nad get my compound
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(1) The 7,893 bp plasmid shown below was digested with a series of restriction enzymes (P = PstI, B = BamHI, S = SalI, X = XbaI). Answer the questions below. a) Indicate
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