Define gelatin - tests for presence of exoenzymatic activity, Biology

Assignment Help:

Explain Gelatin - Tests for Presence of Exoenzymatic Activity?

Gelatin is an incomplete protein as it lacks amino acid tryptophan. It is a major component of connective tissue and tendons in humans and other animals. Many microorganisms produce an enzyme gelatinase, which result in its hydrolysis. Gelatin deep tubes (Nutrient broth supplemented with 12% gelatin) are used to demonstrate the hydrolytic activity of gelatinase. Gelatin is solid at temperature less than 25°C and liquid at temperature above 25°C. Gelatinase degrades gelatin to produce amino acids and liquefies gelatin. Once this degradation occurs, gel characteristic is not restored even at 4°C. Following inoculation and incubation for 48 hours, if the culture remains liquid, it indicates fast gelatin hydrolysis. If it gets solidified, further incubate for 5 days. If it remain liquefy on refrigeration for 30 minutes, it indicates slow gelatin hydrolysis.


Related Discussions:- Define gelatin - tests for presence of exoenzymatic activity

Explain about sex chromosomes, What is the other name given to sex chromos...

What is the other name given to sex chromosomes? Sex chromosomes are also known as allosomes (the other chromosomes that are not sex chromosomes are known as autosomes).

Are nematodes diploblastic or triploblastic animals, Are nematodes diplobla...

Are nematodes diploblastic or triploblastic animals? Just like platyhelminthes, nematodes are triploblastics, i.e., they show three germ layers (ectoderm, mesoderm and endoder

Connective tissue, how connective tissues are like an estuary

how connective tissues are like an estuary

Agro industrial-evaluation of feedstuff, Evaluation of feedstuff In th...

Evaluation of feedstuff In the feed manufacturing process evaluation of the feed ingredients is very important in achieving consistent quality throughout the year. If one is a

How vitamin k used to prevents bone loss, How Vitamin K used to Prevents bo...

How Vitamin K used to Prevents bone loss? Vitamin K is known to inhibit bone loss through inhibiting effect on osteoclast formation. Thus, adequate levels of vitamin K must be

Show cardiovascular risk factors, Q. Show Cardiovascular Risk Factors? ...

Q. Show Cardiovascular Risk Factors? It must be coming in your mind several times that why do some people suffer from heart disease while others do not? Well the most obvious r

Explain ancient greeks and romans - plant classification, Explain History o...

Explain History of Plant Classification - Ancient Greeks and Romans? Hippocrates, "The Father of Medicine" (460-377 B.C.) is reputed to have been one of Democritus 's disciples

Vitamins necessity, Vitamins necessity Vitamins are essential organi...

Vitamins necessity Vitamins are essential organic substances. They are micro nutrients and required in small quantities. They do not produce any energy by themselves.

Entamoeba histolytica, Entamoeba Histolytica Losch (1875), for the fir...

Entamoeba Histolytica Losch (1875), for the first time, described the disease symptoms of infection of Entamoeba histolytica. However, it was only in 1903 that Schaudin gave t

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd