Define gas liquid chromatography, Biology

Assignment Help:

Define Gas liquid chromatography?

This is used for separating and identifying volatile compounds of fatty acids, hydrocarbons, sterols, amino acids and lipids, even from nanogram quantities of a sample. Since volatility is an essential criterion for this method, the compounds should be non-polar or made non-polar through chemical derivatization.

The vapourized mixture of volatile compounds are carried by an inert gas like helium (He), argon (Ar) or nitrogen (N2) (mobile phase) through a long hot tube containing a column of inert fibre brick or diatomaceous earth particles impregnated with a non-volatile liquid like silicone oil, grease (stationary phase). The volatile components separate from each other according to their partition coefficients between the mobile gaseous phase and the stationary liquid phase. As the compounds leave the column they pass through a detector which is linked viz. an amplifier to a chart recorder which in turn records a peak.


Related Discussions:- Define gas liquid chromatography

What is karyotype, What is karyotype? Name the karyotype is given to th...

What is karyotype? Name the karyotype is given to the set of chromosomes of an individual, generally when visualized and identified under the microscope. The visualization in g

What is the function of the umbilical cord, What is the function of the umb...

What is the function of the umbilical cord? The umbilical cord haves blood vessels which convey blood among the fetus and the placenta.

How to indicate an av block, During the course of a physiology laboratory, ...

During the course of a physiology laboratory, a student finds that her PR interval is 0.24 second. Concerned, she takes her own ECG again an hour later and sees an area of the ECG

Diabetic foot are impaired blood supply to foot, Diabetic foot are impaired...

Diabetic foot are impaired blood supply to foot Diabetic foot are impaired blood supply to foot i.e. vasculopathy. This leads to ischaemia which in turn leads to delayed or non

How alkaline cleaning compounds works, Q. How Alkaline cleaning compounds w...

Q. How Alkaline cleaning compounds works? Carbonates, bicarbonates, hydroxides of various metals are called alkaline compounds. Alkaline cleaning compounds have a pH between

Express the term ctene, Express the term ctene. This unique locomotory ...

Express the term ctene. This unique locomotory structure is found in Ctenophora and is made up of fused cilia arranged into flattened plates. Ctenes are then organized into eig

Explain about the lactation process, Explain about the Lactation Process? ...

Explain about the Lactation Process? Lactation is a physiologic process which has profound relevance for both the mother and the newborn. It is the period following pregnancy w

What is hyperhomocysteinaemia, What is Hyperhomocysteinaemia ? Homocyst...

What is Hyperhomocysteinaemia ? Homocysteine has been considered as a risk factor for Cardio-vascular diseases because of many epidemiological and observational studies showing

Classes of life form, Classes of Life Form The five life form classes ...

Classes of Life Form The five life form classes are: Phanerophytes, Chamaephytes, Hemicryptophytes, Cryptophytes, and Therophytes.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd