Define gas liquid chromatography, Biology

Assignment Help:

Define Gas liquid chromatography?

This is used for separating and identifying volatile compounds of fatty acids, hydrocarbons, sterols, amino acids and lipids, even from nanogram quantities of a sample. Since volatility is an essential criterion for this method, the compounds should be non-polar or made non-polar through chemical derivatization.

The vapourized mixture of volatile compounds are carried by an inert gas like helium (He), argon (Ar) or nitrogen (N2) (mobile phase) through a long hot tube containing a column of inert fibre brick or diatomaceous earth particles impregnated with a non-volatile liquid like silicone oil, grease (stationary phase). The volatile components separate from each other according to their partition coefficients between the mobile gaseous phase and the stationary liquid phase. As the compounds leave the column they pass through a detector which is linked viz. an amplifier to a chart recorder which in turn records a peak.


Related Discussions:- Define gas liquid chromatography

How much protein is in the red blood cell, I need a list of equipment neces...

I need a list of equipment necessary to complete it as well as a method to isolate the cell parts, how much protein is in the red blood cell as well as the amount of DNA in the cel

Benthic zone - organisation of the marine ecosystem, Benthic Zone - Organis...

Benthic Zone - Organisation of the Marine Ecosystem The benthic zone is divisible into sub zones horizontally. These are depicted in a cross section portion of the marine habi

What do you mean by trachea, Q. Why doesn't the food enter the trachea inst...

Q. Why doesn't the food enter the trachea instead of going to the esophagus? When food is swallowed the swallow reflex is activated and the larynx closes and elevates to avoid

Accidents resulting in death, Accidents Resulting in Death Accidents t...

Accidents Resulting in Death Accidents that cause death are dealt with in a different way. The law requires full compensation to the widow for complete life or until remarriag

What do biomass pyramids represent, What do biomass pyramids represent? ...

What do biomass pyramids represent? Biomass pyramids show the sum of the masses of the individuals that participate in each trophic level of a food chain.

Animal genetics and breeding, POULTRY BREEDING Poultry breeding has acq...

POULTRY BREEDING Poultry breeding has acquired pre-eminence over all other domesticated livestock enterprises. Production level of layer and broiler type of chicken touched spe

Cellular transport, Cellular Transport Cellular transports demote to th...

Cellular Transport Cellular transports demote to the movement of compounds across the outer wall or membrane of the cell. This transport is critical in two respects. Firstly, t

Identify the hormones produced by the anterior, Identify the hormones produ...

Identify the hormones produced by the anterior and posterior lobes of the pituitary gland and specify the functions of those hormones.

What is the clitellum of earthtworms, Q. What is the clitellum of earthtwor...

Q. What is the clitellum of earthtworms and where it is located? The clitellum is a special region of the annelid constituted by rings (metameres) with reproductive function. I

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd