Define gas liquid chromatography, Biology

Assignment Help:

Define Gas liquid chromatography?

This is used for separating and identifying volatile compounds of fatty acids, hydrocarbons, sterols, amino acids and lipids, even from nanogram quantities of a sample. Since volatility is an essential criterion for this method, the compounds should be non-polar or made non-polar through chemical derivatization.

The vapourized mixture of volatile compounds are carried by an inert gas like helium (He), argon (Ar) or nitrogen (N2) (mobile phase) through a long hot tube containing a column of inert fibre brick or diatomaceous earth particles impregnated with a non-volatile liquid like silicone oil, grease (stationary phase). The volatile components separate from each other according to their partition coefficients between the mobile gaseous phase and the stationary liquid phase. As the compounds leave the column they pass through a detector which is linked viz. an amplifier to a chart recorder which in turn records a peak.


Related Discussions:- Define gas liquid chromatography

Determine the significance of lingual nerve and artery, Significance of Lin...

Significance of Lingual nerve and artery Because the nerve lies medial to the retromolar pad, incisions in this region should remain lateral to the pad and mucosal reflection s

Define citrate utilization test - imvic test, Define Citrate Utilization Te...

Define Citrate Utilization Test - imvic test? Several microorganisms have the ability to make use of citrate as the sole source of carbon and energy. This ability relies on the

Roles of fatty acid, Fatty acids have four main biological roles: 1.  Th...

Fatty acids have four main biological roles: 1.  They are used to create sphingolipids and glycerophospholipids which are necessary elements of biological membranes; 2.  Nume

Explain the recommended dietary allowances - nutrition, Explain the Recomme...

Explain the Recommended Dietary Allowances - Nutrition? RDA: The RDA is the average daily dietary intake that is sufficient to meet the nutrient requirement of nearly all healt

Regulatory factors, 1. Describe how the hypothalamus regulates the secretio...

1. Describe how the hypothalamus regulates the secretion of anterior pituitary hormones and identify all of the individual hypothalamic regulatory factors. Describe how the hypotha

Explain pyruvate carboxylation, Pyruvate carboxylation Pyruvate carbox...

Pyruvate carboxylation Pyruvate carboxylation : In this reaction, pyruvate, CO,  and ATP are converted to oxaloacetate, ADP and Pi catalysed by the enzymepyruvate carboxylase

Genetics, how does the universality of the genetic code make recombinant DN...

how does the universality of the genetic code make recombinant DNA technology possible

DNA, The following series of nucleotide bases forms part of a DNA molecule:...

The following series of nucleotide bases forms part of a DNA molecule: T A C T T A T G A C A C A G G A G G A C T a) Convert this string of bases that would be found on the corresp

Degradation of ecosystem, Degradation of Ecosystem Ancient man was si...

Degradation of Ecosystem Ancient man was simple-minded food gatherer and hunter. He looked upon nature with awe and respect and in fact he worshipped it. But from the time he

Biodeterioration, is paper broken down by microorganisms

is paper broken down by microorganisms

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd