Define gas liquid chromatography, Biology

Assignment Help:

Define Gas liquid chromatography?

This is used for separating and identifying volatile compounds of fatty acids, hydrocarbons, sterols, amino acids and lipids, even from nanogram quantities of a sample. Since volatility is an essential criterion for this method, the compounds should be non-polar or made non-polar through chemical derivatization.

The vapourized mixture of volatile compounds are carried by an inert gas like helium (He), argon (Ar) or nitrogen (N2) (mobile phase) through a long hot tube containing a column of inert fibre brick or diatomaceous earth particles impregnated with a non-volatile liquid like silicone oil, grease (stationary phase). The volatile components separate from each other according to their partition coefficients between the mobile gaseous phase and the stationary liquid phase. As the compounds leave the column they pass through a detector which is linked viz. an amplifier to a chart recorder which in turn records a peak.


Related Discussions:- Define gas liquid chromatography

Define effect of caffeine on athletes, Define effect of Caffeine on athlete...

Define effect of Caffeine on athletes? Caffeine is found in coffee, tea, colas and chocolates. Its doses at 3- 6 mg/d have been known to increase muscle contractility and aerob

What is lyophobic, What is lyophobic If the affinity of the dispersed p...

What is lyophobic If the affinity of the dispersed phase to go into or to remain in colloidal dispersion is slight, the dispersed phase is said to be lyophobic (solvent repelli

What is the echocardiogram, What is the Echocardiogram ? When doubts pe...

What is the Echocardiogram ? When doubts persist whether a patient has CHD or not despite a thorough clinical exam and chest X-ray, ECG, and hyperoxia test, an echocardiogram s

Briefly explain about the flexed-arm hang test, Briefly explain about the F...

Briefly explain about the Flexed-arm Hang Test? Persons who cannot do one pull-up may do the flexed-arm hang. Using the same hand position as in pull-ups, subject takes flexed-

Access cavity perforation - types of root perforation, Access Cavity Perfor...

Access Cavity Perforation - Types of Root Perforation Can occur during access cavity perforation (specially in anterior) Due to misdirection of bur during access cavit

Preventive steps for global warming, Preventive steps for global warming : ...

Preventive steps for global warming :  Growing trees in vacant places. Growing social forests and protect them. Promoting abundant growth of phytoplankton plant species in marine

Gas exchange, Gas Exchange Gill surface area must be large enough to p...

Gas Exchange Gill surface area must be large enough to provide adequate exchange of gases. Therefore, highly active fish have largest relative gill area. Figure compares highl

Epithelial cells of the kidney proximal tubule, Which of the following is t...

Which of the following is true for the epithelial cells of the kidney proximal tubule? A. The sodium-glucose co-transporter in the luminal membrane is responsible for the net f

Define the indications for root-end resection apicoectomy, Define the Indic...

Define the Indications for Root-End Resection Apicoectomy a) Persistent..... "Exactly the SAME of Curettage except the biopsy. b) When the apical portion of the root canal s

Fats, what are fats

what are fats

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd