Define gas liquid chromatography, Biology

Assignment Help:

Define Gas liquid chromatography?

This is used for separating and identifying volatile compounds of fatty acids, hydrocarbons, sterols, amino acids and lipids, even from nanogram quantities of a sample. Since volatility is an essential criterion for this method, the compounds should be non-polar or made non-polar through chemical derivatization.

The vapourized mixture of volatile compounds are carried by an inert gas like helium (He), argon (Ar) or nitrogen (N2) (mobile phase) through a long hot tube containing a column of inert fibre brick or diatomaceous earth particles impregnated with a non-volatile liquid like silicone oil, grease (stationary phase). The volatile components separate from each other according to their partition coefficients between the mobile gaseous phase and the stationary liquid phase. As the compounds leave the column they pass through a detector which is linked viz. an amplifier to a chart recorder which in turn records a peak.


Related Discussions:- Define gas liquid chromatography

Is expression of cloned gene always a success, Question 1 Is expression of...

Question 1 Is expression of cloned gene always a success? How does the method of molecular photo copying work? Question 2 Explain the control of gene expression with the exam

Osmoregulation, explain osmoregulation in crustacean ?

explain osmoregulation in crustacean ?

Can pathophysiology causes cardiovascular disease, Q. Can Pathophysiology c...

Q. Can Pathophysiology causes cardiovascular disease? Cigarette use activates platelets, increases circulating fibrinogen, increases heart rate, and elevates blood pressure. It

Essential for photosynthesis, Aim : To prove that light is essential for ph...

Aim : To prove that light is essential for photosynthesis. Apparatus : A potted plant, a light screen, beaker, iodine solution. Procedure : Keep a potted plant in darkness for tw

Sausage classification - meat products, S a u s a g e classification ...

S a u s a g e classification Classification                                      Examples Cooked-smoked                               Frankfurters, Bologna, Mortadel

Chromolipid, CHROMOLIPID Formed by lipid & pigment, eg. carotene, xanth...

CHROMOLIPID Formed by lipid & pigment, eg. carotene, xanthophylls. Lycopene present in tomato & red chilli. Carrot is rich in b-carotene, converted into vitamin A.

Determine the exercises require to do diabetic patient, Determine the Exerc...

Determine the Exercises require to do diabetic patient Exercises are any bodily activity that enhances or maintain physical fitness and overall health. It is performed for stre

What are the symptoms of mitral stenosis, Q. What are the Symptoms of Mitra...

Q. What are the Symptoms of Mitral stenosis? The cardinal symptom of mitral stenosis is dyspnoea on exertion. Typically it progresses over a period of years. As the severity in

Explain phylum bryophyta, Phylum Bryophyta The Bryophytes include the m...

Phylum Bryophyta The Bryophytes include the mosses and their close relatives. They are widely diverse and grow in a variety of place. 1) Life cycle shows alternation of gene

What thiamine defiency will result in decreased activity, A thiamine defien...

A thiamine defiency will result in decreased activity of: -G-6 phosphase -transketolase -fructokinase

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd