Define gas liquid chromatography, Biology

Assignment Help:

Define Gas liquid chromatography?

This is used for separating and identifying volatile compounds of fatty acids, hydrocarbons, sterols, amino acids and lipids, even from nanogram quantities of a sample. Since volatility is an essential criterion for this method, the compounds should be non-polar or made non-polar through chemical derivatization.

The vapourized mixture of volatile compounds are carried by an inert gas like helium (He), argon (Ar) or nitrogen (N2) (mobile phase) through a long hot tube containing a column of inert fibre brick or diatomaceous earth particles impregnated with a non-volatile liquid like silicone oil, grease (stationary phase). The volatile components separate from each other according to their partition coefficients between the mobile gaseous phase and the stationary liquid phase. As the compounds leave the column they pass through a detector which is linked viz. an amplifier to a chart recorder which in turn records a peak.


Related Discussions:- Define gas liquid chromatography

Explain in brief about the cell membrane, Explain in brief about the Cell M...

Explain in brief about the Cell Membrane All cells, whether prokaryotic or eukaryotic, are bound by a limiting membrane called the cell membrane or the plasma membrane. The ce

Describe how micrornas are able to influence gene expression, 1. Transcript...

1. Transcriptional analyses of eukaryotic cells reveal widespread production of RNA. Using specific examples describe how: a) microRNAs are able to influence gene expression.

Explain the scanning electron microscope, Explain the Scanning Electron Mic...

Explain the Scanning Electron Microscope It can be used to observe intact cells or cell components directly. Then sections are not necessary. It is used for visualizing surface

Explain the main steps in the mongoose bioassay, Question : Explain the...

Question : Explain the main steps in the mongoose bioassay and the mouse bioassay as conducted for testing ciguatera in Mauritius? (i) Provide a brief description on the beh

Respiration , why should breathe faster after a hard work

why should breathe faster after a hard work

Is gene a triplet of consecutive dna nucleotides, Is gene a triplet of cons...

Is gene a triplet of consecutive DNA nucleotides A gene isn't a triplet of DNA nucleotides with their respective nitrogen-containing bases, like CGT or AAG. The nucleotide trip

Define factors that affecting the bmr, Define Factors that Affecting the BM...

Define Factors that Affecting the BMR? Basal metabolic rate, we know, is the largest component of the daily energy demand representing 45 to 70 percent of daily total energy ex

Taxonomy, what is the taxonomic classification for the protist Archaeplasti...

what is the taxonomic classification for the protist Archaeplastida?

Arteries - circulation, Arteries - Circulation The arteries deliver bl...

Arteries - Circulation The arteries deliver blood from the heart. Figure shows the structure of arteries and the different layers of the vessel wall. The thick walls of these

Pollination, Pollination Many flowering plants rely on animals such as b...

Pollination Many flowering plants rely on animals such as bees, butterflies, moths, wasps, beetles, birds, and bats for pollination to produce fruit. Thirty percent of our food

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd