Define gas liquid chromatography, Biology

Assignment Help:

Define Gas liquid chromatography?

This is used for separating and identifying volatile compounds of fatty acids, hydrocarbons, sterols, amino acids and lipids, even from nanogram quantities of a sample. Since volatility is an essential criterion for this method, the compounds should be non-polar or made non-polar through chemical derivatization.

The vapourized mixture of volatile compounds are carried by an inert gas like helium (He), argon (Ar) or nitrogen (N2) (mobile phase) through a long hot tube containing a column of inert fibre brick or diatomaceous earth particles impregnated with a non-volatile liquid like silicone oil, grease (stationary phase). The volatile components separate from each other according to their partition coefficients between the mobile gaseous phase and the stationary liquid phase. As the compounds leave the column they pass through a detector which is linked viz. an amplifier to a chart recorder which in turn records a peak.


Related Discussions:- Define gas liquid chromatography

Explain clinical stigmata of syndromes in heart disease, Explain Clinical s...

Explain Clinical stigmata of specific syndromes in heart disease? Clinical stigmata of specific syndromes: Evidence of preseilce of chromosoinal anomalies or other syndromes

Explain gastric bypass - surgical management for obesity, Explain Gastric B...

Explain Gastric Bypass - surgical management for obesity? Surgery is the current 'gold standard' for bariatric surgical procedures. It involves use of a stapling device to crea

Why alkaline phosphatases use water as a second substrate, Alkaline Phospha...

Alkaline Phosphatases use water as a second substrate. Why do we still treat their kinetics as if they were for a single-substrate reaction?

Define the term archenteron, Define the term Archenteron ? The name give...

Define the term Archenteron ? The name given to the primitive gut, the first tube that runs through the developing embryo and is open to the external environment. Formed during

State diploblastic in brief, State Diploblastic in brief. Organisms for...

State Diploblastic in brief. Organisms formed from only two primitive cell layers- ectoderm and endoderm. Though there may be some type of a matrix between two cell layers, gen

What is immune complex, It is a complex of antibody bound to antigen, which...

It is a complex of antibody bound to antigen, which contains complement components.

What are other important human diseases caused by protozoans, What are othe...

What are other important human diseases caused by protozoans? Some other significant protozoan infections are amebiasis, trichomoniasis, giardiasis, leishmaniasis, toxoplasmosi

What is the secondary structure of a protein, The secondary protein structu...

The secondary protein structure is produced by the manner its amino acids interact by intermolecular bond. These interactions make a spatial conformation of the polypeptide filamen

Circulatory systems in animals, Circulatory Systems in Animals In anim...

Circulatory Systems in Animals In animals that have efficient circulatory systems and readily permeable vascular skins, gas exchange occurs through the integument. Thus we fin

Ancillary and sanitary accomodation, Ancillary Accomodation: Ward Kit...

Ancillary Accomodation: Ward KitchenIPantry:  It should have large sink and drain board along with fly proof door. If  the food is received from central kitchen and tray pre

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd