Define gas liquid chromatography, Biology

Assignment Help:

Define Gas liquid chromatography?

This is used for separating and identifying volatile compounds of fatty acids, hydrocarbons, sterols, amino acids and lipids, even from nanogram quantities of a sample. Since volatility is an essential criterion for this method, the compounds should be non-polar or made non-polar through chemical derivatization.

The vapourized mixture of volatile compounds are carried by an inert gas like helium (He), argon (Ar) or nitrogen (N2) (mobile phase) through a long hot tube containing a column of inert fibre brick or diatomaceous earth particles impregnated with a non-volatile liquid like silicone oil, grease (stationary phase). The volatile components separate from each other according to their partition coefficients between the mobile gaseous phase and the stationary liquid phase. As the compounds leave the column they pass through a detector which is linked viz. an amplifier to a chart recorder which in turn records a peak.


Related Discussions:- Define gas liquid chromatography

Coronal disassembly, Coronal disassembly - Coronal restoration in the ...

Coronal disassembly - Coronal restoration in the retreatment phase called coronal deassembling or disassemblying. - Coronal deassembling means removal of coronal and radi

How does the breathing process correct acidosis, Q. How does the breathing ...

Q. How does the breathing process correct acidosis? The increment of the respiratory frequency makes the body eliminate more carbon dioxide and to shift the equilibrium of the

Coarctation of aorta, Careful palpation of the upper and lower limb pulses ...

Careful palpation of the upper and lower limb pulses would make one suspect coarctation as the cause of hypertension. The lower limb pulses are weak and delayed. Confirmation ca

Signify gene interaction effects, The type of gene interaction in which eff...

The type of gene interaction in which effects of one gene override or mask the effects of other entirely different genes is known as: a) Mutation b) Linkage c) Pleitropy

Feeding mechanisms of animals, Feeding Mechanisms of Animals All anima...

Feeding Mechanisms of Animals All animals have evolved successful methods for extracting their required nutrition from the environment. Thus we find a diversity of feeding mec

Explain about the mucor - fungi, Explain about the Mucor  - Fungi? Muc...

Explain about the Mucor  - Fungi? Mucor is present as a food contaminant. It resembles Rhizopus in life history and colonies appear just like Rhizopus. However, important diffe

Electrochemical gradient, Describe forces that contribute to cell''s electr...

Describe forces that contribute to cell''s electrochemical gradient. How does electrochemical gradient influence ion transport?

Phylum annelida, what so special for phylum annelida

what so special for phylum annelida

Enumerate the term - clinical neuroscience, Enumerate the term - Clinical n...

Enumerate the term - Clinical neuroscience Clinical neuroscience concerns the study of clinical populations both to well understand neuroanatomy and to test psychological theor

What is reflexive memory, What is reflexive memory? Reflexive memory re...

What is reflexive memory? Reflexive memory relies on the cerebellum and amygdala. Compare among aerobic respiration and anaerobic respiration. Aerobic respiration happens in th

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd