Define gas liquid chromatography, Biology

Assignment Help:

Define Gas liquid chromatography?

This is used for separating and identifying volatile compounds of fatty acids, hydrocarbons, sterols, amino acids and lipids, even from nanogram quantities of a sample. Since volatility is an essential criterion for this method, the compounds should be non-polar or made non-polar through chemical derivatization.

The vapourized mixture of volatile compounds are carried by an inert gas like helium (He), argon (Ar) or nitrogen (N2) (mobile phase) through a long hot tube containing a column of inert fibre brick or diatomaceous earth particles impregnated with a non-volatile liquid like silicone oil, grease (stationary phase). The volatile components separate from each other according to their partition coefficients between the mobile gaseous phase and the stationary liquid phase. As the compounds leave the column they pass through a detector which is linked viz. an amplifier to a chart recorder which in turn records a peak.


Related Discussions:- Define gas liquid chromatography

Explain homogenization, Explain Homogenization Homogenized milk will n...

Explain Homogenization Homogenized milk will not be affected, as the fat globules are already broken up.  Homogenization increases the viscosity of whole milk but slightly dec

What is end-to-end anastomosis subclavian flap aortoplasty, What is End-to-...

What is End-to-End Anastomosis with Subclavian Flap Aortoplasty ? The two operations can be combined as a single procedure. Medially end-to-end anastomosis is done between the

Determine the categories of Taxonomic breakdown, For convenience, living th...

For convenience, living things are placed into different groups. Taxonomic breakdown of living things comprises the following categories: Family, Class, Genus, Phylum, Order, K

Illustrate fluoxetine is an antidepressant drug or not, Fluoxetine is an an...

Fluoxetine is an antidepressant drug that presents an action mechanism related to the synaptic transmission. What is that mechanism? Fluoxetine is a substance that inhibits th

Determine the atp-sensitive potassium channel, ATP-sensitive potassium chan...

ATP-sensitive potassium channel A. The channel is a spanning protein with a receptor site for ATP located on an intracellular region of the protein. B. When blood plasma lev

Plasmodesmata, PLASMODESMATA Termed by Tangl & Strasburger. Cytop...

PLASMODESMATA Termed by Tangl & Strasburger. Cytoplasmic / Protoplasmic bridges between 2 adjacent cells. Desmotubules connects the E.R. of 2 cells. Plasmodesmata p

Define fatty acid synthesis, Fatty acid synthesis is the formation of fatty...

Fatty acid synthesis is the formation of fatty acids from malonyl-CoA and acetyl-CoA precursors by action of enzymes called as fatty acid synthases. It is a very significant part o

Explain about steatorrhoea, Q. Explain about Steatorrhoea? Steatorrhoea...

Q. Explain about Steatorrhoea? Steatorrhoea is a symptom of the disorders of fat metabolism and malabsorption syndrome and can be defined as n condition of foul-smelling bulky

What is echocardiography, Q. What is Echocardiography? Echocardiography...

Q. What is Echocardiography? Echocardiography in paediatric cardiology forms a different category from that of adult echo with acquired heart disease. Some of views which pro

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd