Define gas liquid chromatography, Biology

Assignment Help:

Define Gas liquid chromatography?

This is used for separating and identifying volatile compounds of fatty acids, hydrocarbons, sterols, amino acids and lipids, even from nanogram quantities of a sample. Since volatility is an essential criterion for this method, the compounds should be non-polar or made non-polar through chemical derivatization.

The vapourized mixture of volatile compounds are carried by an inert gas like helium (He), argon (Ar) or nitrogen (N2) (mobile phase) through a long hot tube containing a column of inert fibre brick or diatomaceous earth particles impregnated with a non-volatile liquid like silicone oil, grease (stationary phase). The volatile components separate from each other according to their partition coefficients between the mobile gaseous phase and the stationary liquid phase. As the compounds leave the column they pass through a detector which is linked viz. an amplifier to a chart recorder which in turn records a peak.


Related Discussions:- Define gas liquid chromatography

Haccp plan, HACCP  Plan  :  The written document which  is  based upon the...

HACCP  Plan  :  The written document which  is  based upon the principles  of  HACCP  and which delineates  the procedures  to  be  followed.

By which term liverworts and mosses are characterized, Liverworts and mosse...

Liverworts and mosses are characterized by their lack of vascular conducting tissue. These two groups of liverwort, plants and mosses, are known by which of the below terms: a)

Influence of lv function, Influence of LV Function :  LV functions, whethe...

Influence of LV Function :  LV functions, whether normal or impaired is important while selecting a patient for CABG and for long-term results of surgical revascularisation. If le

Dietary guidelines fur hyperlipidemic patients, Q. Dietary Guidelines fur h...

Q. Dietary Guidelines fur hyperlipidemic patients? • Calories: to maintain ideal body weight. • Carbohydrates should constitute 55-65% of calories with emphasis on • polysacc

Skeletal system - girdles, GIRDLES - (i ) PECTORAL GIRDLE - ...

GIRDLES - (i ) PECTORAL GIRDLE - 4 bones. It is located on posterolateral part of upper region of the throax. It consists of scapula & clavicle. Scapula is placed

Describe chemical equation of photosynthesis?, Q. What is the chemical equa...

Q. What is the chemical equation of photosynthesis? The chemical equation of photosynthesis is given below: 6 CO 2 + 6 H 2 O + light --> C 6 H 12 O 6 + 6 O 2

How to identify the monomer or molecular components, Please help with the f...

Please help with the following: For each of the four macromolecules carbs, lipids, proteins and nucleic acids, please identify the monomer or molecular components and name the b

Technique-ross operation--surgical techniques, Technique : The suitability...

Technique : The suitability of the pulmonary autograft for aortic valve replacement has to be studied by accurate measurement by echocardiogram of the aortic and pulmonary annuli.

Human physiology, which bones forms lhe non moving muscle attachment in

which bones forms lhe non moving muscle attachment in

Pre-operative nursing management of cardiac surgical patient, PRE-OPERATIVE...

PRE-OPERATIVE NURSING MANAGEMENT OF CARDIAC SURGICAL PATIENTS Cardiac surgical patients generally get investigated thoroughly before getting referred to the surgeons. Many of

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd