Define gas liquid chromatography, Biology

Assignment Help:

Define Gas liquid chromatography?

This is used for separating and identifying volatile compounds of fatty acids, hydrocarbons, sterols, amino acids and lipids, even from nanogram quantities of a sample. Since volatility is an essential criterion for this method, the compounds should be non-polar or made non-polar through chemical derivatization.

The vapourized mixture of volatile compounds are carried by an inert gas like helium (He), argon (Ar) or nitrogen (N2) (mobile phase) through a long hot tube containing a column of inert fibre brick or diatomaceous earth particles impregnated with a non-volatile liquid like silicone oil, grease (stationary phase). The volatile components separate from each other according to their partition coefficients between the mobile gaseous phase and the stationary liquid phase. As the compounds leave the column they pass through a detector which is linked viz. an amplifier to a chart recorder which in turn records a peak.


Related Discussions:- Define gas liquid chromatography

Define complete assessment for dietary management - surgery, Define Complet...

Define Complete Assessment for Dietary Management during Surgery? A complete assessment must include: Physical examination (anthropornetric measurements such as ideal/us

Frog, Frog fema reproductive system

Frog fema reproductive system

Define stomata, What are the stomata? How these structures participate in t...

What are the stomata? How these structures participate in the plant transpiration? The Stomata (singular, stoma) are small specialized passages for gases and water present in t

Sample outline of child history questionnaire, Sample Outline of Child Hist...

Sample Outline of Child History Questionnaire Basic identifying data: Child's name Date of birth Date of evaluation Person referring for evaluation Per

Types of synovial joint, TYPES OF SYNOVIAL JOINT - 1 .      BALL AND ...

TYPES OF SYNOVIAL JOINT - 1 .      BALL AND SOCKET JOINT - One bone forms a ball like head that fits into a socket formed in the other bone. Movement is possible in al

Modern cell theory, MODER N CELL THEORY Also known as cell doctrine or...

MODER N CELL THEORY Also known as cell doctrine or cell principle. [Cell theory + cell lineage theory = cell principle] Schleiden-Schwann's cell theory and cell lineage

The polymerase chain reaction, The Polymerase Chain Reaction Kary Mulli...

The Polymerase Chain Reaction Kary Mullis established the technique of the polymerase chain reaction (PCR) in the year of 1983. He won a Nobel Prize for the procedure in the ye

Pulmonary artery diastolic pressure, PADP may be determined by measuring th...

PADP may be determined by measuring the end diastolic velocity of PADP = 4 V 2 Pulmonary regurgitant signal and applying the following formula V is  the end diastolic veloci

Determine the importance of behavioural observations, Determine the importa...

Determine the importance of Behavioural observations Behavioural observations are often noteworthy to the extent that they involve alterations in the examiner's usual response

What are the causes of constipation, Q. What are the Causes of constipation...

Q. What are the Causes of constipation? The most common causes of constipation are poor elimination habits, a lack of fibre in the diet, insufficient fluid intake, lack of exer

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd