Define gas liquid chromatography, Biology

Assignment Help:

Define Gas liquid chromatography?

This is used for separating and identifying volatile compounds of fatty acids, hydrocarbons, sterols, amino acids and lipids, even from nanogram quantities of a sample. Since volatility is an essential criterion for this method, the compounds should be non-polar or made non-polar through chemical derivatization.

The vapourized mixture of volatile compounds are carried by an inert gas like helium (He), argon (Ar) or nitrogen (N2) (mobile phase) through a long hot tube containing a column of inert fibre brick or diatomaceous earth particles impregnated with a non-volatile liquid like silicone oil, grease (stationary phase). The volatile components separate from each other according to their partition coefficients between the mobile gaseous phase and the stationary liquid phase. As the compounds leave the column they pass through a detector which is linked viz. an amplifier to a chart recorder which in turn records a peak.


Related Discussions:- Define gas liquid chromatography

Oil gas, Composition of oil gas is CH 4 = 25-30%; H 2 = 50-55%; CO = 10-1...

Composition of oil gas is CH 4 = 25-30%; H 2 = 50-55%; CO = 10-12%; CO 2 = 3% It calorific value is 4500-5400 kcl/m 3 . It is used as laboratory gas. Oil gas is prepared b

Classify suture materials, Classify suture materials Sutures can broad...

Classify suture materials Sutures can broadly be classified into two kinds - Absorbable: will break down harmlessly in the body over time without intervention, Non-absor

Genetics, The involuntary muscle movement is also genetical sk question #M...

The involuntary muscle movement is also genetical sk question #Minimum 100 words accepted#

Historical development of psychiatric nursing, HISTORICAL DEVELOPMENT OF PS...

HISTORICAL DEVELOPMENT OF PSYCHIATRIC NURSING: History is a  record of opportunities, recognized and informed,  that produce profound changes in a given field. History of psyc

Describe a method of diagnosing diabetes mellitus, Q. In ancient Greece the...

Q. In ancient Greece the father of Medicine, Hypocrates, described a method of diagnosing diabetes mellitus by tasting the patient's urine. What is the physiological explanation fo

What is plaque, What is plaque? Plaque is a film over the teeth and hav...

What is plaque? Plaque is a film over the teeth and having of saliva, mucus, bacteria and the substances they produce. It may also have mineral salts of calcium and magnesium.

Explain zidovudine, Zidovudine Zidovudine is available alone and in fix...

Zidovudine Zidovudine is available alone and in fixed-dose combinations with lamivudine as Combivir and with lamivudine and abacavir as Trizivir. It can be given in combination

Which are growth tissues of plants, Which are growth tissues of plants? How...

Which are growth tissues of plants? How do they categorize and where can they be found? Growth tissues of the plants are the meristems. The Meristems are the tissues that produ

What are the major human diseases caused by roundworms, Q. What are the maj...

Q. What are the major human diseases caused by roundworms? The major human diseases caused by nematodes are ancylostomiasis, ascariasis (hookworm infection) and filariasis (com

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd