Define gas liquid chromatography, Biology

Assignment Help:

Define Gas liquid chromatography?

This is used for separating and identifying volatile compounds of fatty acids, hydrocarbons, sterols, amino acids and lipids, even from nanogram quantities of a sample. Since volatility is an essential criterion for this method, the compounds should be non-polar or made non-polar through chemical derivatization.

The vapourized mixture of volatile compounds are carried by an inert gas like helium (He), argon (Ar) or nitrogen (N2) (mobile phase) through a long hot tube containing a column of inert fibre brick or diatomaceous earth particles impregnated with a non-volatile liquid like silicone oil, grease (stationary phase). The volatile components separate from each other according to their partition coefficients between the mobile gaseous phase and the stationary liquid phase. As the compounds leave the column they pass through a detector which is linked viz. an amplifier to a chart recorder which in turn records a peak.


Related Discussions:- Define gas liquid chromatography

Define the types of canned foods, Define the Types of Canned Foods? 1....

Define the Types of Canned Foods? 1. Some foods form convection currents when being heated inside a can and so are heated faster since self-mixing - as foods become more visco

Classic procedure- mitral valve replacement, Classic Procedure: The app...

Classic Procedure: The approach is the same as described earlier for open mitral valvotomy. The excision starts with an anterior incision on the anterior leaflet at 12o'clock p

Importance of mendelism, IMPORTANCE OF MENDELISM 1 .       Improvement...

IMPORTANCE OF MENDELISM 1 .       Improvement of Plants Hybridization is used for obtaining improved varieties of plants. This process results in combinations of desirable

Explain the photosensitive detectors, Explain the Photosensitive Detectors?...

Explain the Photosensitive Detectors? A wide range of detectors varying in senstivity and cost are available. They contain a light sensitive surface that releases electrons in

Polypeptide chains of hemoglobin, Q. In sickle cell anemia, a hereditary di...

Q. In sickle cell anemia, a hereditary disease, there is replacement of one amino acid by another in one of the four polypeptide chains of hemoglobin. In this case are all of the s

What do you mean by cori cycling, What do you mean by Cori Cycling? In ...

What do you mean by Cori Cycling? In this cycle, glucose released by peripheral tissues is metabolized to lactate, which is then resynthesized to glucose in the liver. This pro

What is the magnitude n, Three point charges are located at the corners of ...

Three point charges are located at the corners of an equilateral triangle. Find the magnitude and direction of the net electric force on the 1.60 uC charge. (Let A = 1.60 uC, B= 6.

Failure of public production, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Periphyton - aquatic ecosystem, Periphyton - Aquatic Ecosystem These a...

Periphyton - Aquatic Ecosystem These are organisms which remain attached or clinging to stems and leaves of rooted plants or substances emerging above the bottom mud. Usually

Disposal of microorganisms, Disposal : You must always sterilize cultures ...

Disposal : You must always sterilize cultures and contaminated instruments and glassware before disposing them of or before washing-up. Usually, you can do this by autoclaving or

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd