Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

Microbiology of air, Ask question #i need the complete table having the nam...

Ask question #i need the complete table having the names of the diseases their causative agents and their control,caused by air-borne pathogens?

Long-term responses - behaviour of plants, Long-term responses - Behaviour ...

Long-term responses - Behaviour of Plants Plants alter their course of development to suit the prevailing environmental conditions. For example, leaf maturation and fall, onse

What is the neuromuscular synapse, What is the neuromuscular synapse? N...

What is the neuromuscular synapse? Neuromuscular synapse is the structure by which the neural impulse passes from the axon of a motor neuron to the muscle cell. This structure

Maintain nutrition in diarrhoea, Maintain nutrition Your responsibilit...

Maintain nutrition Your responsibility as a nurse is to take care of nutrition and prevent the child from starvation. Breast feeding'should be continued during the diarrhoea e

Autoradiography, Autoradiography Autoradiography is a modification of t...

Autoradiography Autoradiography is a modification of the radioactive tracer technique. In this technique, the radioactivity is detected by a thin film of photographic emulsion

Explain bleeding on probing, Bleeding on Probing (BOP) BOP when elicite...

Bleeding on Probing (BOP) BOP when elicited (notated in patient record as BOP +) after the insertion of a probe into the sulcus with light pressure i.e. 25 N has been shown to

Explain viscosity and plasticity of colloidal particle, Viscosity and Plast...

Viscosity and Plasticity of colloidal particle Various degrees of viscosity and plasticity are encountered in colloids. Viscosity may be described as resistance to pouring.

Define nutrient requirements during emergencies, Define Nutrient Requiremen...

Define Nutrient Requirements during Emergencies? The nutritional requirements of the people do change during conditions of unforeseen stress and/or any calamity. Certain recomm

What are ions, What are ions? What are the two types of molecules into whic...

What are ions? What are the two types of molecules into which ions are classified? Ions are atoms or substances electrically charged by means of loss or gain of electrons.

Explain restriction endonucleases are enzymes, Restriction endonucleases ar...

Restriction endonucleases are enzymes which: 1. make cuts at specific positions within the DNA molecule 2. recognize a specific nucleotide sequence for binding of DNA ligase

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd