Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

Endodermis , Endodermis is the layer of cells surrounding the vascular cyl...

Endodermis is the layer of cells surrounding the vascular cylinder of plants.

Define bioactive substances from protein foods, Define Bioactive Substances...

Define Bioactive Substances from Protein Foods? Bioactive substances are derived from living organisms that can be used by humans for a variety of applications. They are consti

Define mobilization of bone calcium and phosphorous, Define Mobilization of...

Define Mobilization of bone calcium and phosphorous? It is now firmly established that vitamin D 3 is metabloized first in the liver to 25-hydroxyvitamin D (25 OH- D) (calcicl

Crustacea, Crustacea The Crustacea show a great morphological diversit...

Crustacea The Crustacea show a great morphological diversity of appendages which may be grouped into three categories, cephalic, thoracic and abdominal. Their appendages are c

Automated dna sequencing, Automated DNA sequencing is now common place whic...

Automated DNA sequencing is now common place which is based on the chain termination method but using a fluorescent dye attached to an oligonucleotide primer instead of using radio

Gases are exchanged when living organisms breathe, Which two gases are exch...

Which two gases are exchanged when living organisms breathe? Oxygen and carbon dioxide are the gases exchanged when living organisms breathe.

Effect of air pollution, (i)    Effect on Human Health: Air pollution...

(i)    Effect on Human Health: Air pollution causes many respiratory problems in human being. (1)   Dust, soot and smog causes several respiratory troubles such as bronc

Agro industrial-chopping and grinding, Chopping/grinding These are the ...

Chopping/grinding These are the processes by which the particle size of cereals, oil cakes and roughages including green fodders and crop residues is reduced employing various

What do you mean by systematic zoology, Q. What do you mean by Systematic Z...

Q. What do you mean by Systematic Zoology ? It contains articles on animals systematic, and papers dealing with cytological attributes of species and higher taxa, distribution p

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd