Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

Describe cytokinesis in a plant cell, Describe cytokinesis in a plant cell....

Describe cytokinesis in a plant cell. Vesicles produced by the Golgi apparatus fuse at  the midline of the cell to form the cell plate, a  cell wall that elongates to divide th

Explain acid and bases, Explain acid and bases? Acids and Bases :  So...

Explain acid and bases? Acids and Bases :  Some aqueous solutions, however, such as HCl, form an excess of hydrogen ions [ H + ] and are called acids. Others, such as NaOH,

Demography, DEMOGRAPH Y - Scientific study of vital & social statistic...

DEMOGRAPH Y - Scientific study of vital & social statistics such as births, deaths, disease etc. of human population is called demography. It deals with - 1.      Change

What is the characteristic of cardiac muscle, What are the characteristics ...

What are the characteristics of cardiac muscle that allow it to be highly resistant to fatifue?

Pre pregnancy counselling, Pre Pregnancy Counselling To prevent excess...

Pre Pregnancy Counselling To prevent excess spontaneous abortions and congenital malformations in infants of diabetic mothers, diabetic care, education and counselling must be

Deficiency diseases, DEFICIENCY DISEASES Animals may suffer from diseases...

DEFICIENCY DISEASES Animals may suffer from diseases caused by deficiencies of energy and proteins, mineral nutrients and vitamins, which generally occur either due to deficiency

Number of risk factors of gout, Q. Number of risk factors of gout? A nu...

Q. Number of risk factors of gout? A number of risk factors are related to the development of hyperuricemia and gout. These factors include: • Hereditary: Genetics may play

Other agro-industrial byproducts, Other agro-industrial byproducts Sup...

Other agro-industrial byproducts Supply of nutrients in the livestock ration can be maintained by using the locally available industrial byproducts, which in spite of having h

Nucleus, NUCLEUS First described and christened by Robert brown 1831, a...

NUCLEUS First described and christened by Robert brown 1831, a nucleus is the largest and most important organelle of eukaryotic cells. It is membrane bound and generally locat

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd