Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define Free Solution or Moving Boundary Method?
The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.
Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.
Ventricular Septal Defect (VSD) In ventricular septal defect there is abnormal communication between right and left ventricle. Anatomically 90 per cent of all VSD are located
Wet and dry thermal treatment Wet thermal treatment: it is based on exposure of shredded infectious waste to high temperature, high pressure steam. It is inappropriate for the
Explain the Safe Requirement of nutritional needs? Given the individual variations in nutritional requirements that have been discussed earlier, the lowest continuing intake le
How are mutagenic agents related to cancer incidence in a population? Is cancer a disease transmitted to the individual offspring? An exposition of a population to mutagenic ag
WHY NETWORKING IN HOSPITALS Sharing of data is essential, especially in any kind of hospital setting. For staff and doctors of a hospital, if they have details of patients rea
Assume for this question that we are discussing a rare human disorder. Describe as detailed as possible the characteristics of this disorder if it is: autosomal dominant autosomal
State four long-term benefits of exercise. The long-term advantages of exercise are: a) Enhance in size of the muscles used, b) Decrease of heart rate and c) Enhance
What is Atrial Septal Defect ? Figure: Anatomical location of Am Indication for Surgery : The presence of an ASD or PAPVC (Partial Anomalous Pulmonary Venous Conn
Define Food Effects in the Survival & Growth of Microorganism? Nature of the food also affects the microbial survival and growth. Each food has its own characteristics which de
Explain the Ethological Approach in Taxonomy The use of behavioural or ethoIogica1 characteristics in animals is relatively new, though extremely useful, particularly in the id
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd