Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define Free Solution or Moving Boundary Method?
The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.
Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.
A patient has a low red blood cell count, and microscopic examination reveals an abnormally high proportion of circulating reticulocytes. Upon subsequent examination, the patient i
Explain what is Light Microscope - Microscopy Modern light microscopes are compound microscopes. Here the magnified image formed by the objective lens is further enlarged by on
Question 1 Write a short note on the following 1 Angiosperms 2 Meristematic tissue Question 2 What is ecosystem? List and explain
In studies of human body, which of the following terms will be used to explain the lack of voluntary control of urination or voiding of bladder? Is it a) Anuria b) Incontine
What are the typical vegetation and the typical fauna of the tundra? Tundra has vegetation formed mainly by mosses and lichens. In the fauna the densely furred animals, like ca
Through the year of 1960s, the gene was clearly explained as the region of DNA which gives increase to a single polypeptide or to a one RNA for genes whose final product is RNA not
Biological Stress Since in nature, the various organisms do not live in complete isolation from others, stress to a plant species might also be caused by what other organisms
Which of the subsequent statements concerning platelets is INCORRECT. Platelets: a) Are between 1/2 and 1/3 the diameter of the red cell b) Are roughly disk-shaped c) Hav
Central Nervous System (CNS) Covering of the brain and spinal cord is called Meanings (membranes which cover and protect brain and spinal cord are called meanings) There is flu
In the early days of microbiology, the disease producing submicroscopic agents were termed as 'filterable viruses' because of their ability to pass through conventional filters whi
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd