Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

Explain about suspensions, Explain about Suspensions Sol is a colloidal...

Explain about Suspensions Sol is a colloidal system, in which solid particles are dispersed in a liquid. When the particles of a solid are separated into large aggregates of pa

Mechanical valves, Mechanical Valves They are made of a combination o...

Mechanical Valves They are made of a combination of metal alloys, pyrolite carbon and dacron. Types of Mechanical Valves Caged-ball Valve (Star-Edwards) A me

Regular soda are made with aluminium, If the cans of regular soda are made ...

If the cans of regular soda are made with aluminium than diet soda, then regular soda will sink. Write brief descriptions of how you could test this hypothesis.

Define features of fusarium - fungi and yeast, Define features of Fusarium ...

Define features of Fusarium - Identification of Fungi and Yeasts? Identifying features of Fusarium: 1. Wooly, white fuzzy colonies changing colour to pink, purple or yellow.

Define protein requirement for cancer patients, Define Protein Requirement ...

Define Protein Requirement for Cancer Patients? Both the metabolic stress of cancer, as well as, chemotherapy results in increased tissue catabolism. Hypoalbuminemia and anaemi

What will happen to resting membrane potential, What will happen to resting...

What will happen to resting membrane potential if potassium succinate were injected into the intracellular environment (cytoplasm) of a neuron?

Genetic, 1. The genes for ruby eyes (rb), tan body (t) and cut wings (ct) a...

1. The genes for ruby eyes (rb), tan body (t) and cut wings (ct) are all found on the X-chromosome of Drosophila melanogaster. All of these are recessive traits. They map in the or

Define the prevalence and incidence of bulimia nervosa, Define the Prevalen...

Define the Prevalence and Incidence of bulimia nervosa? Bulimia nervosa appears to have become more prevalent during the past 30 years. We do not have much data on Indian popul

Agro industrial-incriminating factors in feeds, Agro Industrial-Incriminati...

Agro Industrial-Incriminating factors in feeds There are many anti-nutritional factors present in feeds and fodders which affect the utilization of nutrients. Some of these are

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd