Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

Effects of cardiogenic pulmonary edema, Effects of Cardiogenic Pulmonary Ed...

Effects of Cardiogenic Pulmonary Edema Interference with oxygen transfer in the lungs Depression arterial oxygen tension Sense of suffocation and oppression in the

Phylum , what are diploblastic organisms?

what are diploblastic organisms?

How do respiratory pigments act, How do respiratory pigments act? Respi...

How do respiratory pigments act? Respiratory pigments are oxygen-carrying molecules present in the blood. When the oxygen concentration is high, for instance, in the pulmonary

Differences between single-unit and multi-unit muscles, DIFFERENCE S BETWE...

DIFFERENCE S BETWEEN SINGLE-UNIT AND MULTI-UNIT SMOOTH MUSCLES     Single-unit Smooth Muscles   Multi-uni t Smooth Muscles

Ethylene - apical dominance, Ethylene - Apical Dominance Ethylene also...

Ethylene - Apical Dominance Ethylene also plays a role in the inhibition of lateral bud growth. It is present in those tissues like shoot apex where auxins are also present. I

What are antioxidants, What are antioxidants? To put it simply, antioxi...

What are antioxidants? To put it simply, antioxidants are important naturally occurring nutrients, (vitamins, minerals) which help to protect body from certain types of cancers

How to investigate aortic stenosis by cardiaccatheterization, Q. How to inv...

Q. How to investigate aortic stenosis by Cardiac Catheterization? The indications are to confirm hemodynamics when the non-invasive tests are not conclusive or there is a disc

Which blood vessels carry venous blood to the heart, Q. What are the blood ...

Q. What are the blood vessels that carry venous blood to the heart? What is the blood vessel that collects arterial blood from the heart? The blood vessels that debouch in the

What are the functional divisions of the nervous system, Q. What are the fu...

Q. What are the functional divisions of the nervous system? Functionally the nervous system can be divided into the visceral nervous system and somatic nervous system. The s

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd