Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

What are the main gas exchange organs of the plants, What are the main gas ...

What are the main gas exchange organs of the plants? How is the process accomplished? In the covering of the leaves and of the primary structure of the stem gas exchange is mad

#title.zoology., #question.why obelia is considered of special interest in ...

#question.why obelia is considered of special interest in zoology.

Protein synthesis with dna, Protein Synthesis with DNA The central dogm...

Protein Synthesis with DNA The central dogma of modern biochemistry is totally based on the coded information holded within deoxyribonucleic acid (DNA). Double stranded DNA is

Explain functional properties of food lipids, Functional properties of food...

Functional properties of food lipids Chemically, fats and oils, as you may already know by now, are a combination of glycerol and fatty acids. The glycerol molecule has three s

Prior to the solving of the structure of dna, Prior to the solving of the s...

Prior to the solving of the structure of DNA, Erwin Chargaff calculated the amounts of the four nucleotides (A, T,G,C) and made which of the following conclusions? A. All four

Explain the occurrence of vitamin B2, Explain the Occurrence of vitamin B 2...

Explain the Occurrence of vitamin B 2 Vitamin B 2 (riboflavin) occurs in nature almost exclusively in a combined form i.e. esterified with phosphoric acid as riboflavin-5'-ph

Zoo201, In an outline form only, attempt the classification of the phylum a...

In an outline form only, attempt the classification of the phylum arthropoda

Design a controlled experiment, Design a controlled experiment to test the ...

Design a controlled experiment to test the hypothesis that acid precipitate inhibits the growth of elodea, a common freshwater plant.

Etiological factor of diverticular disease, Q. Etiological factor of divert...

Q. Etiological factor of diverticular disease? The causes of diverticular disease are not certain, but several factors may contribute to changes in the wall of the colon. These

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd