Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

Determine the excitation threshold concerning neurons, How different are th...

How different are the concepts of action potential, resting potential and excitation threshold concerning neurons? Action potential is the maximum positive voltage level achiev

Driving force - mineral nutrition, Driving Force - Mineral Nutrition ...

Driving Force - Mineral Nutrition Let us now find out what is the driving force involved in protein mediated transport. Many membrane transport proteins allow specific solute

PHYLUM ANNELIDA, GERENAL CHARACTERICS OF PHYLUM ANNELIDA

GERENAL CHARACTERICS OF PHYLUM ANNELIDA

Ecology.., write the charecteristics of lake ecosystem

write the charecteristics of lake ecosystem

Explain adverse effects of valacyclovir, Adverse Effects of Valacyclovir  ...

Adverse Effects of Valacyclovir  Valacyclovir is generally well tolerated; adverse effects are similar to those with acyclovir. GI disturbance, headache, rash, CNS effects such

What are some prophylactic measures for tapeworm infections, What are some ...

What are some prophylactic measures for tapeworm infections? The main prophylactic measures against taeniasis and cysticercosis are: not to ingest raw or badly cooked swine or

Explain phylum phytofagellata, Phylum Phytofagellata (Plant flagellates...

Phylum Phytofagellata (Plant flagellates) 1) At some stage of life cycle they have one or more flagella. The flagella are used for locomotion. 2) Photosynthetic protistan

Anti platelet drugs-peri operative problems, Anti Platelet Drugs In t...

Anti Platelet Drugs In the early years of CABG, patients used to be put on aspirin and persantin (Dipyridamole). Persantin is not routinely prescribed now. Aspirin dose can b

Explain development of excretory system, Explain Development of excretory s...

Explain Development of excretory system? The filtration rate of kidney is low and infants find it difficult to eliminate high concentration of solutes. However, by the end of t

Cyclins and cyclin-dependent kinases, Rhythmic fluctuations in the abundanc...

Rhythmic fluctuations in the abundance and activity of cell-cycle control molecules pace the events of the cell cycle. • Kinase - a protein which activates or deactivates another

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd