Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define Free Solution or Moving Boundary Method?
The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.
Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.
Explain Hormonal proteins Hormonal proteins coordinate the bodily activities. Various peptide and protein hormones (such as insulin and growth hormone) carry information tha
Operative requirements for stage i surgery Although Implant placement under general anesthesia must be done in an operating theatre with complete resources, most implant surger
Who was Charles Darwin? Charles Darwin was an English naturalist born in 1809 and considered the father of the theory of evolution. At the end of the year 1831, before turning 23
Emerging and New Generation By-Products Oil palm by-products are good examples of NCFR which are becoming increasingly important new feeds. This is associated with the rapidly
Explain Advantages and Disadvantages of Spread Plate Method? The advantages and disadvantages of spread plate method are highlighted next. Advantages 1. It is useful fo
Explain the difference between numerical chromosome abnormalities and single gene disorders. Describe methods of diagnosing chromosome abnormalities prenatally and after birth. How
Feeding mechanism in holozoic animal
Explain Xanthan Gum Xanthan gum is a microbial polymer prepared commercially in pure culture fermentation from Xanthomonas campestris. This polysaccharide was given the generic
physical properties of protoplasm
What are the endocrine functions of the placenta? The placenta has endocrine function since it secretes the hormones progesterone and estrogen that maintain the endometrium (in
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd