Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

Define interferons - humoral mechanisms, Define Interferons - Humoral Mecha...

Define Interferons - Humoral Mechanisms Interferons are soluble proteins secreted naturally when cells become infected by foreign bodies. Infected lymphocytes release interfero

Explain the term fasb and iasb, Discuss FASB and IASB. Comment on at least ...

Discuss FASB and IASB. Comment on at least two specific aspect of the two standard setting boards and their role in setting accounting.

What is the mechanism of elisa, ELIZA stands for enzyme linked immune diffu...

ELIZA stands for enzyme linked immune diffusion assays. It works on standard of antigen antibody reaction.

Characteristics of genetically modified foods, Normal 0 false ...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

What are genotypes and phenotypes, The agouti locus in mice has three allel...

The agouti locus in mice has three alleles: A is recessive and confers a black coat, A is dominant to a and confers an agouti coat, and AY is dominant to both and confers a yellow

Cells, List structures found in unicellular organisms that are an adaptatio...

List structures found in unicellular organisms that are an adaptation to their free-living life and are not usually found in the cells of multicellular organisms.

Chloroplasts, CHLOROPLASTS Schimper (1883) coined the term chlorop...

CHLOROPLASTS Schimper (1883) coined the term chloroplastid for green plastids. Mayer called them Autoplast. Chloroplast name proposed by Erera. Green plastids wi

Essential features of health educator, Essential Features of Health Educa...

Essential Features of Health Educator The health'educator should: be confident about the subject matter,  be able to converse skillfully in the language which is un

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd