Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

Explain phylum rhodophyta, Phylum Rhodophyta (Red algae) 1) The photosy...

Phylum Rhodophyta (Red algae) 1) The photosynthetic pigments include red pigments (phycoerythrin) and blue pigment (phycocyanin) apart from chlorophyll, of which red pigment pr

What is mucoperiosteal flap, What is mucoperiosteal flap Silk is easy t...

What is mucoperiosteal flap Silk is easy to handle, ties with a slip knot, and is relatively inexpensive compared with other nonabsorbable suture materials currently available.

Instances of human diseases caused by bacteria, Q What are instances of hum...

Q What are instances of human diseases caused by bacteria? Some human diseases caused by bacteria are tuberculosis, pertussis, diphtheria, gonorrhea, bacterial meningitis, bubo

Explain the parasite-amoeba, Normal 0 false false false ...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Synthetic petrol, Petrol or gasoline can be synthesized by following three ...

Petrol or gasoline can be synthesized by following three methods: 1.      Polymerisation 2.      Fischer - tropsch method 3.      Bergius process polymerisation In

Define that dna is the genetic material, How did Alfred Hershey and Martha ...

How did Alfred Hershey and Martha chase arrive at the conclusion that DNA is the Genetic material?

Determine the photographic analysis, Photographic Analysis Documentatio...

Photographic Analysis Documentation is critical in any clinical procedure and more importantly in this field where due to the cost of the treatment involved, there is little ma

Effects of long term malnutrition on children, Effects of long term malnutr...

Effects of long term malnutrition on children The effects of long time malnutrition are more severe in children. Unlike adults children do not have sufficient amounts of c

Tanco beans plant, what are the origin, morphology, active constituents, us...

what are the origin, morphology, active constituents, uses and market preparation of tanco beans ?

Energy absorbed by plants, Normal 0 false false false E...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd