Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

Myelinated fibre, Myelinated fibre Axon is a relatively long process (...

Myelinated fibre Axon is a relatively long process (also quite often referred to as nerve fibre) and can be considered as functionally specialised for conduction of excitation

Determine the significance of siphuncle., Determine the significance of Sip...

Determine the significance of Siphuncle. A cord of tissue that passes between different chambers of a Nautilid shell. Siphuncle regulates the amount of air or fluid in chambers

Natural selection, Darwin put fort the notion of the survival of the fittes...

Darwin put fort the notion of the survival of the fittest. How do we define 'fittest'? The answer usually given is those who survive. So you can readily see that this is a circular

Explain the sensory respiratory center - feature of medulla, Explain the Se...

Explain the Sensory Respiratory center - Feature of Medulla Respiratory center: The center for regulating rate and depth of respiration is present in the medulla. Here, nerve i

Radiography, RADIOGRAPHY You  have read  in GNM courses about radiographi...

RADIOGRAPHY You  have read  in GNM courses about radiographical examination. In this text the discussion will be on the following radiographical tests.   Chest Roentgenogram

Competition - processes in succession , Competition - Processes in Successi...

Competition - Processes in Succession The aggregation of individuals in an area leads to inter specific and intra specific competition. The competition is usually for

What is binary fission in cell reproduction, What is Binary Fission in cell...

What is Binary Fission in cell reproduction? Cell division takes place in prokaryotic cells by binary fission, also called prokaryotic fission. In prokaryotes, DNA is contained

How different are fecundation in osteichthyes, How different are fecundatio...

How different are fecundation in osteichthyes and in chondrichthyes? In chondrichthyes fecundation is internal by means of copulation. In osteichthyes fecundation generally is

Briefly explain about the chromium, Briefly Explain about the Chromium? ...

Briefly Explain about the Chromium? As you will go through this section, you will realize that compared to other minerals, the essentiality of chromium was recognized very late

Closed style - style of stigma interaction, Closed Style - Style of Stigma ...

Closed Style - Style of Stigma Interaction Cotton shows an epidermis with stomata, a cortex of thin-walled parenchyma with several vascular bundles and strands of transmitting

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd