Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

What are the main characteristics of the bryophytes, What are the main char...

What are the main characteristics of the bryophytes? Bryophytes are nonvascular plants, i.e., they do not have conductive tissues and they perform transport of water and nutrie

Create a punnett square, Q. Assume the gene for dimples, d, is recessive. T...

Q. Assume the gene for dimples, d, is recessive. Then assume that two individuals with the genotypes Dd and dd mate. Create a Punnett square for this mating and state the probabili

Explain food applications of agar, Food Applications of Agar The bakery...

Food Applications of Agar The bakery industry has been the largest user  of agar because of its heat- resistant gel properties. Confectionary products, such as agar jelly candi

Explain the term of monosaccharides, Explain the term of Monosaccharides? ...

Explain the term of Monosaccharides? Monosaccharides :  The simplest carbohydrates are Monosaccharides, simple sugars. These generally have three to six carbon atoms, and con

Phylum:Echinodermata , What classifies an animal into the Echinodermata phy...

What classifies an animal into the Echinodermata phylum?

Metabolic process location, Metabolic Process Location Within the study...

Metabolic Process Location Within the study of biochemistry, what tends to obtain lost amidst the numerous pathways and enzymes is the fact which metabolism concerns the overal

Fever, Fever influenza

Fever influenza

Define transcription phase of gene expression process, Define Transcription...

Define Transcription Phase of Gene Expression Process? Transcription is the enzyme synthesis of RNA on a DNA template. This is the first stage in the overall process of gene ex

Define the process of digestion of fats, Define the Process of Digestion of...

Define the Process of Digestion of Fats? The enzyme in human gut which is responsible for fat digestion is 'lipase', Lipase is secreted by both stomach and pancreas. In stomach

What raw materials require for the manufacture of margarine, What raw mater...

What raw materials require for the manufacture of margarine Soybean and peanut (or groundnut) oils are of great economic importance. Refined soybean oil contains branched fura

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd