Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

Human lungs, Human lungs A pair of spongy, elastic and bag like stru...

Human lungs A pair of spongy, elastic and bag like structures of lungs is present in the chest cavity, one on either side of the heart. Lungs are enclosed by a double lay

Horse diseases-african horse sickness, African horse sickness African h...

African horse sickness African horse sickness (AHS) is a highly fatal insect born viral disease of equidae caused by an Orbivirus (family Reoviridae) and characterized by sever

Light stress in chloroplasts, Light Stress in Chloroplasts As mentione...

Light Stress in Chloroplasts As mentioned earlier, very high light intensity can inhibit photosynthesis due to accumulation of excess of excitation energy. A portion of this r

Gram positive & gram negative bacteria , What is the difference among Gram ...

What is the difference among Gram positive & Gram negative bacteria based on cell content? Ans) The cell membrane is made if more of lipoproteins in gram negative compared to gr

What are the major natural plant hormones and their effect, What are the ma...

What are the major natural plant hormones and what are their respective effects? The major natural plant hormones and their respective functions are the following- Auxins that

Explain food habits - diet presciption, Food Habits Food Habits: These ...

Food Habits Food Habits: These must be known  so that the diets  can  be  recommended keeping in mind the  food preferences  of  the patient. Whether a person is  a vegetarian

Excretion in animals, Excretion in Animals Excretion is concerned alon...

Excretion in Animals Excretion is concerned along with the removal of metabolic wastes that arise as a result of oxidation of energy rich compounds and metabolism of proteins

Define traditional methods of food processing, Define Traditional Methods o...

Define Traditional Methods of Food Processing? Because food is so important to survival and most foods remain edible for only a brief period of time, people as the initial ages

Calculate micronutrient and other nutrient requirements, Calculate Micronut...

Calculate Micronutrient and other specific nutrient requirements? The recommended average daily per capita intake of various specific nutrients for typical population requiring

Endothelium derived constricting factor, Endothelium is a source of vasocon...

Endothelium is a source of vasoconstrictor factor also. The best characterized of these are the endothelins. Endothelium produces only ET1. Unlike NO, which is released rapidly in

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd