Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

Determine the transplantation of mitochondria, Based on your reading of the...

Based on your reading of the article "Hello Mothers, Hello Father" which of the following is a correct statement regarding the transplantation of mitochondria? A. The sperm cel

The concentration of purified dna, In an experiment, it was necessary that ...

In an experiment, it was necessary that you determine the concentration of dsDNA by using a spectrophotometer. You used 6 microliters of your purified dsDNA, and the reading at OD=

Illustrate about human eyes, Eyes We have a pair of eyes. They are held...

Eyes We have a pair of eyes. They are held in socket of the skull by four rectus muscles and two oblique muscles. These muscles control the movements of the eyes. The wall of t

How gender affects the bmr, How Gender affects the bmr? We have already...

How Gender affects the bmr? We have already emphasized earlier that sex difference in metabolic rates are primarily attributable to difference in body size and composition. Wom

Define typical ambient air pollutants, Define Typical Ambient Air Pollutant...

Define Typical Ambient Air Pollutants Particulate matter Sulfur containing compounds Organic compounds Nitrogen containing compounds Carbon monoxide

Pancreatic juice resume the digestion of carbohydrates, Q. How does the pan...

Q. How does the pancreatic juice resume the digestion of carbohydrates? What is the involved enzyme? Carbohydrate digestion begins with the action of the salivary amylase (ptya

Reptiles, biting mechanism in snakes

biting mechanism in snakes

Vitality and vigour - qualitative characters, Vitality and Vigour - Qualita...

Vitality and Vigour - Qualitative Characters Vitality is related to the condition of a plant and its capacity to complete its life cycle, while vigor refers more specifically

Properties of receptors, Properties of Receptors The receptors collect...

Properties of Receptors The receptors collect information concerning the change in the environment such as temperature, illumination etc. In lower animals, sensory perception

What is the etiological agent, What is the etiological agent and the main m...

What is the etiological agent and the main manifestations of cholera? Cholera is a bacterial disease caused by the Vibrio cholerae. The disease is transmitted by fecal-oral rou

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd