Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

Relationship of different systems in diabetes mellitus, Describe the relati...

Describe the relationship of different systems in diabetes mellitus Diabetes Mellitus has direct relationship with endocrine system, especially with endocrine part of the panc

Antonmy, bone modeling may occur?

bone modeling may occur?

What is bicuspid aortic, What is bicuspid aortic? Bicuspid aortic val...

What is bicuspid aortic? Bicuspid aortic valve is the most common form of CHD seen in adults. Isolated bicuspid aortic valves may be stenotic or non-stenotic to start with. T

Procedure for preparation of selective & differential medium, Procedure for...

Procedure for Preparation of Selective and Differential Medium Now prepare the MacConkey agar medium following the steps given herewith: 1. Weigh and dissolve all ingredient

Metestrus - estrous cycle, Metestrus - Estrous cycle This occurs shortly...

Metestrus - Estrous cycle This occurs shortly after ovulation and is intermediate between estrus and diestrus. The period lasts for 10 to 14 hours and mating is usually not perm

How do we treat protein energy malnutrition?, How do we Treat Protein Energ...

How do we Treat Protein Energy Malnutrition? Major objective of the treatment of PEM is to provide adequate energy and protein intake and control infections, if any. Mild and m

Legal level -pollution control, Legal level -Pollution control Polluti...

Legal level -Pollution control Pollution must be controlled by effective legislation also. Like USA, Japan, Germany and many other countries a comprehensive legislation for pr

Define the dietary requirements of phosphorus, Define the Dietary Requireme...

Define the Dietary Requirements of phosphorus? We read in the previous section that the requirements of phosphorus are closely linked with those of calcium. The phosphorus req

Determine the wechsler intelligence scales, Determine the Wechsler Intellig...

Determine the Wechsler Intelligence Scales This test is given according to manual instructions and is not modified in any way. Most clinicians use the most current revision of

Etiological factor of diverticular disease, Q. Etiological factor of divert...

Q. Etiological factor of diverticular disease? The causes of diverticular disease are not certain, but several factors may contribute to changes in the wall of the colon. These

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd