Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

How are radioactive probes useful in dna fingerprinting, How are radioactiv...

How are radioactive probes useful in DNA fingerprinting? Radioactive probes bind to specific fragments in a DNA sample like a donor gene. A probe can identify which bacteria ha

What are the main causes of mutation, Q. What are the main causes of Mutati...

Q. What are the main causes of Mutation? Some mutations take place randomly and spontaneously during process of mitosis or meiosis. Others are caused by mutagenic agents that a

What are the pupillary defects, What are the Pupillary defects There ar...

What are the Pupillary defects There are certain deviations  from normal pupillary reflexes which point towards certain specific pathology.

Does ploidy reduction occur in mitosis, During which meiosis division does ...

During which meiosis division does ploidy reduction occur? Does ploidy reduction occur in mitosis? In the cell division by meiosis ploidy reduction occurs in meiosis II. Initia

Explain differential media - culture media, Explain Differential Media - Cu...

Explain Differential Media - Culture Media? It allows differentiation among morphologically and biochemically related group of organisms. It contains certain ingredients which

Explain some advantages of most probable number, Explain some Advantages of...

Explain some Advantages of Most Probable Number? 1. It is simple and method of choice for determining fecal coliform densities. 2. Results of different laboratories are comp

Injury resulting from an accident, Injury Resulting From an Accident ...

Injury Resulting From an Accident Accident was described earlier which meant that accident was a sudden event. However, several legal opinions favored exclusion of suddenness

Sympatric speciation, Sympatric speciation can be regarded as speciation wh...

Sympatric speciation can be regarded as speciation where parent species gives rise to a daughter species without the individuals of a species being separated by space or territory.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd