Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

Ablation experiment, Ablation experiment  is an experiment designed to prod...

Ablation experiment  is an experiment designed to produce an animal deficient in one or the few cell types, in order to study cell lineage or the cell function. The logic is to mak

How does the presence of exoskeleton, Q How does the presence of exoskeleto...

Q How does the presence of exoskeleton describe the general small size of arthropods? Since they have periodic ecdysis and exoskeleton, the growth of arthropods is limited to a

Explain susceptible organisms of tubeculosis, Susceptible Organisms Wh...

Susceptible Organisms When infection proves to be caused by a fully susceptible strain of TB, the initial phase of treatment should include isoniazid, rifampin and pyrazinamid

Linkage of health with development, Linkage of Health With Development ...

Linkage of Health With Development Health, in its comprehensive or holistic sense, refers to the state of completephysical, mental, social and spiritual well being of a person.

Explain working of cerebral cortex, Q. Explain working of Cerebral Cortex? ...

Q. Explain working of Cerebral Cortex? Cerebral Cortex/Cerebrum: The cerebrum or the cortex is the large part of the brain and is associated with the cognitive functions of the

Transport in human, what is the name of the valve that separates the chambe...

what is the name of the valve that separates the chamber?Why is that valve necessary?

Nutrition, what is the mode of nutrition in tape worm

what is the mode of nutrition in tape worm

Difference between self pollination and cross pollination, What is differen...

What is difference between self pollination and cross pollination? Which of the two modes of pollination contributes more to the plant diversity? Self pollination take places w

Cellular specific immune response, Q. What is the difference between cellul...

Q. What is the difference between cellular specific immune response and humoral specific immune response? Humoral specific immune response is the defense system by means of, an

Active instruments-coronal disassembly devices, Active instruments - Do...

Active instruments - Do groove occlusally or buccally until reach tooth structure - Apply this instrument then screw to break the bond between tooth structure and crown GC

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd