Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

What is osteoblast, What is osteoblast The osteoblast is the key for th...

What is osteoblast The osteoblast is the key for the development of new bone. It is essential that a large number of osteoblasts accumulate on the implant surface(contact osteo

Periods of cultural evouition and modern man, PERIOD S OF CULTURAL EVOUITI...

PERIOD S OF CULTURAL EVOUITION AND MODERN MAN - Paleolithic period: Stone age. Age of tools of stones and bones, cave paintings. Mesolithi c period: Age of domesticati

Protein truncation test, The protein truncation test (ptt) gives an uncommo...

The protein truncation test (ptt) gives an uncommon exception, targeting mutations that create shortened proteins, majorly premature translation termination. Ptt has various attrac

Poultry and duck diseases-duck plague, Duck plague Duck plague, caused ...

Duck plague Duck plague, caused by a member of Herpesviridae, has world wide distribution and occurs among domestic and wild ducks, geese, swans and waterfowls. Epidemiolo

Excretory system - urinary bladder, URINARY BLADDER - Pear shaped sac l...

URINARY BLADDER - Pear shaped sac like. Situated in pelvic region of abdominal cavity. Ventrally placed. Lined by transitional epithelum. Detrusor muscle i.e. to expell out

Define chronic fever and infection, Chronic fever and  infection Chron...

Chronic fever and  infection Chronic fever  and  infection are always supported will a long standing history of symptoms  or repeated episodes of infection. One of the commonl

Water movement through the integument, Normal 0 false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

What is the importance of vitamin B2, What is the Importance of vitamin b2 ...

What is the Importance of vitamin b2 As a prosthetic group of the flavin enzymes, vitamin B 2 is involved in the reactions of almost all nutrients of plants and animals. They

Conditions required for mimicking pericardial effusion, Q. Conditions requi...

Q. Conditions required for Mimicking Pericardial Effusion? In 2DE few conditions can mimic PE. a) Pericardial fat-Mostly localized anteriorly. Absence of fat in the posterio

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd