Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

Write the basic about suturing, Write the basic about Suturing The goa...

Write the basic about Suturing The goal during suturing multiple tissue levels is to suture periosteum to periosteum and tissue to tissue. Pull the suture just tight enough

Explain the expansion of the universe, Using the Raisin Bread model from th...

Using the Raisin Bread model from the textbook, describe the expansion of the universe. Do the galaxies expand also?

Describe the method of molecular photo copying, Question 1: "Plant tiss...

Question 1: "Plant tissue culture now has direct commercial applications" Explain. Describe plant tissue culture Illustrate importance of plant tissue culture Sho

What is arteriole diameter, The axons of all the baroreceptors in the body ...

The axons of all the baroreceptors in the body were destroyed at 2 AM.  All else is normal.  Which of the following statements is true for the system at 2:10 AM when compared to th

Why dairy or milk product are important for human body, Why Dairy or milk p...

Why Dairy or milk product are important for human body? This first food of mammals is rich in body-building proteins and bone-forming calcium, besides being the only source of

What is coronary disease, Q. What is coronary disease? The Coronary dis...

Q. What is coronary disease? The Coronary disease, or the coronary insufficiency, is a disease in which there is total or partial obstruction of one or more of the arteries tha

Pbpk modeling during human drug development, Early human studies are normal...

Early human studies are normally conducted in healthy subjects to assess acute safety and, if applicable, to monitor biochemical and pharmacological responses. These studies also p

What is the tympanum, What is the tympanum? In which part of the ear is it ...

What is the tympanum? In which part of the ear is it located and what is its function? The tympanum (or ear drum) is a membrane located in the middle ear just after the auditor

What is cytokinesis in cell reproduction, what is Cytokinesis in cell repro...

what is Cytokinesis in cell reproduction? Cytokinesis: Mitosis is usually followed by cytokinesis, the division of the cytoplasm and cell membrane. Cellular organelles are no

How to get a stock primer concentration of 100µm, I have 30µg of a primer, ...

I have 30µg of a primer, whose molecular weight is 600. How much water should I add to get a stock primer concentration of 100µM?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd