Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define Free Solution or Moving Boundary Method?
The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.
Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.
Q Which are the organs and characteristics of the digestive system of earthworms related to the kind of diet of these animals? Small organisms ingested together with soil parti
Placement of the Implant in an Infected Socket or a Pathologic Lesion Dental implants may fail due to: 1) Placement of the implant fixture into an infected socket (during im
Describe ASD closure and VSD closure in heart dieases? ASD Closures : Small ASD's can be closed directly. Others will need a pericardial patch. Piimum ASD and Sinus Venosus A
Agricultural activities - Cause of Air Pollution Agricultural activities too are a major cause of air pollution. About 60 to 65% of carbon dioxide is produced from burning of
Natural selection is such a powerful force in evolution because cells with even a small growth advantage quickly outgrow their competitors. To illustrate this process, consider a b
Explain the characterstics of Acute AR Murmur ? Characteristic: Relatively low pitched, decrescendo diastolic murmur, which may end part way through diastole. These murmurs ar
Phototherapy Unit Principle Photoisomerisation and photo-oxidation changes indirect bilirubin into water soluble substance. Operational Instruction Adjust
How can proteins be classified? Give suitable examples. Proteins can be classified based on: Shape and size: fibrous or globular proteins. Functional properties: I
a patient has experienced a bleed in the left frontal lovel of their brain.descibe the effect on motor function for this patient after the bleed.
e use itwhat is difference between peroxisme and lysosome what is function of it and where w
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd