Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

How is genetic determination of sex established in humans, How is the genet...

How is the genetic determination of sex established in humans? In the diploid genome of the human beings, there are 46 chromosomes, 44 of them are autosomes and two are sex chr

Rapport - building, Rapport-Building Here the Counsellor tries to esta...

Rapport-Building Here the Counsellor tries to establish a rapport and gain the client's trust. Let us see how we make friend? When we meet someone we take time to react, we ob

Open heart surgery , Open Heart Surgery These surgeries are done unde...

Open Heart Surgery These surgeries are done under alternative arrangements to continue oxygenated systemic blood supply, while the heart is operated on (Cardiopulmonary bypas

History of evolution, History of evolution? Evolution is usually define...

History of evolution? Evolution is usually defined as "change over time." In spite of the incredible diversity of life found on Earth, many fundamental characteristics are shar

Maintenance in the continuing care cycle, Maintenance in the Continuing Car...

Maintenance in the Continuing Care Cycle Impaired dexterity: Any impairment of dexterity, even if it is temporary, may be detrimental to dental implants because home maintena

Define discovery of other essential nutrients, Define Discovery of other Es...

Define Discovery of other Essential Nutrients? Following the identification of the food factors involved in beriberi, pellagra and scurvy, the search was on for other water-sol

Eggs of insect, EGG S OF INSECT This eggs is centrolecithal and it is ...

EGG S OF INSECT This eggs is centrolecithal and it is elliptical. It diameter is 2-3 mm. It has two eggs membrane i.e. vitelline membrane and chitinous capsule. The chit

What are the two big groups into which cells are classified, Cells are clas...

Cells are classified into eukaryotic or prokaryotic. Prokaryotic cell is that with no a delimited nucleus. Eukaryotic cells are those with nucleus delimited by membrane.

Monocot and dicot plants, project formate for monocot and dicot plants in f...

project formate for monocot and dicot plants in feild per month

Carbohydrate metabolism, CARBOHYDRATE METABOLISM Carbohydrates  are  br...

CARBOHYDRATE METABOLISM Carbohydrates  are  broken down into  monosaccharides which are absorbed into  the  blood stream.  In  the  liver and muscles, most  of  the glucose  is

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd