Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

Evolution of human kind, The evolution of human kind can be regarded as the...

The evolution of human kind can be regarded as the climax of phylogenic history of organisms. In the previous units of this block as well as the previous block we have detailed for

Synthetic Biology, I need analysis of Stza gene in Aspergillus nidulans thr...

I need analysis of Stza gene in Aspergillus nidulans through synthetic biology, OpenWetWare and Biobricks

Human impact on carbon cycle, Human Impact on Carbon Cycle Human activ...

Human Impact on Carbon Cycle Human activities have greatly influenced the carbon cycle. The discharge of CO 2 , into the atmosphere is steadily increasing owing to burning of

Biochemical changes - consequences of aging, Biochemical Changes - Conseque...

Biochemical Changes - Consequences of Aging Several biochemical changes are correlated with aging. Detailed biochemical studies in rats have been carried out by Prof. M.S. Kan

Plants of estuary, Plants of Estuary The plants of the estuary are of ...

Plants of Estuary The plants of the estuary are of four basic types: Phytoplankton, Marginal marsh vegetation, Mud-flat algae and Epiphytic plants

What are some diseases caused by abnormal gh secretion, What are some disea...

What are some diseases caused by abnormal GH secretion by the hypophysis? In childhood deficient GH secretion might be lead to delayed growth and in severe cases to nanism (dwa

What are the reasons of food processing, What are the reasons of Food Proce...

What are the reasons of Food Processing? The reasons for food processing may vary, but the main objectives are to: Preserve the nutritive quality of food by preventing th

What is fixism, What is fixism? Fixism is the theory about the diversit...

What is fixism? Fixism is the theory about the diversity of life on earth that affirms that the current existent species were identical to species of the past and came out alre

In which condition ulcer continues, Complete the sentence by choosing one o...

Complete the sentence by choosing one of the given biological terms. If an ulcer continues and ultimately erodes all the way through the wall of stomach or duodenum, condition

Phylum Protozoa, Summary of the Phylum and protozoa and the important

Summary of the Phylum and protozoa and the important

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd