Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

Difference between temporal summation and spatial summation, Q. What is the...

Q. What is the difference between temporal summation and spatial summation of muscle fibers? What is tetany?   Spatial summation is the recruiting of new muscle fibers to incr

Define the concept of food security, Define the Understanding the Concept o...

Define the Understanding the Concept of Food Security? Food security may be defined as a physical and economic access by all people, at all times, to sufficient food to meet th

What is lipoproteins, A lipoprotein is a biochemical assembly which haves b...

A lipoprotein is a biochemical assembly which haves both lipids and proteins, bound to the proteins that allow fats to move by the water outside and inside cells. The proteins serv

Describe the blood sugar testing, A small drop of blood is required to perf...

A small drop of blood is required to perform the test and this can be easily obtained from the patient's fingertip. A large number of glucometer devices are easily available and t

Illustrate the technique of radiographic template, Technique of radiographi...

Technique of radiographic template 1. Block any severe interdental undercuts or spaces with wax. Soak the patient's diagnostic cast with wax up in slurry. (Slurry is the super

Lipid profile, Abnormalities in the lipid profile are found in patients wit...

Abnormalities in the lipid profile are found in patients with diabetes mellitus. The lipid abnormalities increase the risk of heart disease in diabetics and hence should be regular

SYMBIOTIC THEORY, WHAT IS SYMBIOTIC THEORY. PLEASE EXPLAIN THIS TO ME IN A ...

WHAT IS SYMBIOTIC THEORY. PLEASE EXPLAIN THIS TO ME IN A VERY SIMPLE WAY.

Does the environment exert an influence on the phenotype, Does the environm...

Does the environment exert an influence on the phenotype? A phenotype may be changed (compared to the original situation conditioned by its genotype) by nongenetic means. Examp

Define procedure for testing presence of proteins in milk, Define Procedure...

Define Procedure for Testing the Presence of Proteins in Milk? 1. Add 0.5 ml of alcohol to 0.5 ml milk.  Alcohol helps in curdling the milk. 2. Observe for the curdling of m

Define pulmonary angiography, Q. Define Pulmonary angiography? Pulmonar...

Q. Define Pulmonary angiography? Pulmonary angiography is the angiographic opacification of the main and pulmonary artery and its branches. By radiographic techniques, it is po

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd