Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

Cell theory, Cell Theory The term cell was first used by an English cyt...

Cell Theory The term cell was first used by an English cytologist, Robert Hooke (1665) not for unit protoplasmic masses, but for the well defined and empty compartments, he obs

Silver point and silver point removal-endodontics principles, Silver point:...

Silver point: -    Uses of silver point: ease of handing and placement, ductility, radiopacity,and have  some antibacterial activity. -    Lack of acceptable 3D seal of the ca

Agro industrial-emerging and new generation by-products, Emerging and New G...

Emerging and New Generation By-Products Oil palm by-products are good examples of NCFR which are becoming increasingly important new feeds. This is associated with the rapidly

Explain leaf primordia, Explain Leaf Primordia ? In contrast to the ro...

Explain Leaf Primordia ? In contrast to the root, which produces branches from meristematic tissue called the pericycle, shoots have areas of meristematic tissues called nodes

Into which classes are mollusc divided, Q Into which classes are mollusc di...

Q Into which classes are mollusc divided? What are some representing beings of each class? The phylum Mollusca is divided into five main classes: pelecypods, or bivalves (Pelec

Experiment of an egg osmometer, An egg osmometer Place some dilute hydr...

An egg osmometer Place some dilute hydrochloric acid or strong vinegar in a shallow dish, likeas a saucer, to a depth of about one centimetre. Hold the large end an egg in the

What is prokaryotic chromosomes, The DNA of a bacterial cell,such as Escher...

The DNA of a bacterial cell,such as Escherichia coli is a circular double-stranded molecule  often  referred  to as the bacterial  chromosome.  The circular  DNA  is packaged into

Coevolution of host-parasites, Coevolutionary relationships between parasit...

Coevolutionary relationships between parasites and their hosts can be more complicated than between predators and their prey. As in the case of prey-predator relationship, one can

Explain amylopectin, Amylopectin Amylopectin forms a branched structure...

Amylopectin Amylopectin forms a branched structure with about 30 glucose units in a chain among branches. There are usually slightly more  'outer' unbranched chains (called A-c

Cartilaginous and the osseous tissue, Q. Which kind of tissue are the carti...

Q. Which kind of tissue are the cartilaginous and the osseous tissue? The osseous and the cartilaginous tissues are considered connective tissues since they are tissues in whic

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd