Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

Which hormones secreted by adrenal medulla, Q. What are the hormones secret...

Q. What are the hormones secreted by the adrenal medulla? What are their respective functions? The medullary portion of the adrenals secretes hormones of the catecholamine grou

Which type of cell came first in evolution, Q. Which type of cell came firs...

Q. Which type of cell came first in evolution - the eukaryotic cell or the prokaryotic cell? This is an interesting problem of biological evolution. The majorly accepted hypoth

What are the advantages of intra oral periapical radiographs, Advantages of...

Advantages of Intra Oral Periapical Radiographs a) It is a useful high yield modality for ruling out local bone or dental disease b)  It is of value in identifying critical

How can bacteria produce human insulin, How can bacteria produce human insu...

How can bacteria produce human insulin on an industrial scale? What are the other forms of insulin made available by the pharmaceutical industry? Bacteria do not naturally synt

High energy fixation - nitrogen fixation, High energy fixation - Nitrogen F...

High energy fixation - Nitrogen Fixation Through cosmic radiations, lightning, volcanic activity and meteorite trails which provide the high energy needed to combine atmospher

Explain the birth in human biology, Explain the Birth in human biology? ...

Explain the Birth in human biology? In humans, birth of the infant occurs about 270 days after conception. The period during which the uterus contracts to expel the newborn

Explain in brief about the diseases sleep paralysis, Explain in brief about...

Explain in brief about the diseases Sleep paralysis This is an episode of paralysis in the transition between wakefulness and sleep. The period of paralysis is usually brief bu

Explain water-soluble and fat-soluble vitamins, What is the difference betw...

What is the difference between water-soluble and fat-soluble vitamins? Why can fat-soluble vitamins cause harm when ingested in excess? Water-soluble vitamins are those soluble

Define about the centrifuge, Define about the Centrifuge? Centrifuge is...

Define about the Centrifuge? Centrifuge is an apparatus consisting essentially of a compartment spun about a central axis to separate contained materials of different specific

Name the joint disorders that s more a risk, In Reggie's case, he fractured...

In Reggie's case, he fractured the proximal end of his right femur, an integral component of his hip. Name the joint disorders that Reggie is more a risk of in (a) the short term a

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd