Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define Free Solution or Moving Boundary Method?
The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.
Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.
How can I fuck my girlfriend without taking out cum?
Approaches to Neuropsychological Assessment Probably the most useful model of neuropsychological interpretation is described in Reitan and Wolfson's (1993) four approaches to a
Which of these lists contains the four postulates of natural selection? Which of these lists contains the four postulates of natural selection? Uniform population, heritable trait
Define Nutrition Education for prevention of vitamin a deficiency? Ignorance, you may recall studying earlier, is an important determinant of vitamin A deficiency. There is, th
What is sporopollinin?
name a disorder of human blood that is caused by mutation
What is the stage of cellular respiration during which carbon dioxide is liberated? In aerobic cellular respiration the release of carbon dioxide occurs in the transformation o
Q. Areas affected in peptic ulcer? The areas affected in peptic ulcer (due to erosion) can be: 1. Lower part of the oesophagus. 2, stomach (lesser curvature - an urn, whe
Q. What are the Symptoms of pectic ulcer? Increased gastric tone and painful hunger contraction when stomach is empty. Hunger contraction 1-3 hours after meals is the main com
RADIOISOTOPE DIAGNOSTIC PROCEDURE: Radioisotope diagnostic procedures include perfusion, ventilation and gallium scan. Perfusion Lung Scan Following injection of a radio
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd