Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

What do you mean by taxonomist, Q. What do you mean by Taxonomist? Mode...

Q. What do you mean by Taxonomist? Modern classification systems are based on many types of evidence. A truly natural classification is obtained from analysis and harmonisation

Female accessory sex organs, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Interactions between minerals, Interactions between minerals Copper de...

Interactions between minerals Copper deficiency has been identified as a serious problem for grazing ruminants. A deficiency may be due to low concentrations of Cu in forage a

Determine the organs of the digestive system of earthworms, Which are the c...

Which are the characteristics and organs of the digestive system of earthworms related to the type of diet of these animals? Earthworms eat decomposing organic material and sma

Illustrate about urine of humans, Urine Normal urine is clear, yellow i...

Urine Normal urine is clear, yellow in colour, slightly acidic. Urine consists of 96% water and 4% solids like urea, uric acid, urates, chlorides, phosphates, oxalates, sulphat

Psychosocial problems, PSYCHOSOCIA L PROBLEMS  - 1 .       Accidental...

PSYCHOSOCIA L PROBLEMS  - 1 .       Accidental injury - Automobile accidents are no. 1 killers of adolescents, as they drive often fast. 2 .       Depression - I

Define hormonal responses to injury, Define Hormonal Responses to Injury? ...

Define Hormonal Responses to Injury?  A number of hormonal changes take place in patients following injury. There is a marked rise in the counter regulatory hormones, viz., glu

What do you mean by subxiphoid long axis sweep, Q. What do you mean by Subx...

Q. What do you mean by Subxiphoid Long Axis Sweep? This sweep begins with keeping the transducer at subxiphoid region, positioning directly posterior with marker pointing towar

Photochemical stage of the photosynthesis process, Q. What are the procedur...

Q. What are the procedures of the photochemical stage of the photosynthesis process? Photolysis of water, with liberation of molecular photophosphorylation and oxygen of ADP, w

Anthropodes, advantages of cuticle of anthropodes

advantages of cuticle of anthropodes

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd