Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

How do malign neoplasias appear, Q. How do malign neoplasias appear? Th...

Q. How do malign neoplasias appear? The Neoplasias appear due to DNA mutations in genes that regulate the cellular proliferation thus making the cell lose its capacity to contr

Illustrate about the chemical disinfection, Illustrate about the Chemical d...

Illustrate about the Chemical disinfection Sharp waste may cause injury and person may have diseases like Hepatitis B, Cand HIV/AIDS. Therefore it is important for to understa

Oxidation of glyceraldehyde-3-phosphate, Oxidation of glyceraldehyde-3-phos...

Oxidation of glyceraldehyde-3-phosphate Oxidation of glyceraldehyde-3-phosphate : The  conversion  of glceraldehyde- 3-phosphate to  1,3-bisphosphoglycerate  is catalysed  by

Overgrazing - degradation of ecosystem, Overgrazing - Degradation of Ecosys...

Overgrazing - Degradation of Ecosystem It refers to the condition when the grazing pressure on the vegetation is so intense that it does not recover. Ultimately, there is a lo

What is etiopathology, Q. What is Etiopathology? Who is likely to devel...

Q. What is Etiopathology? Who is likely to develop gout? What are the risk factors? Let us find out. Gout is caused when there is over production of uric acid in normal purine

Favus, Favus Fowl favus is a superficial, chronic, sporadic mycotic in...

Favus Fowl favus is a superficial, chronic, sporadic mycotic infection of birds caused mainly by Trichophyton gallinae. Very rarely Trichophyton simii is also involved in the

Food chain and types of food chain, In an ecosystem various trophic levels ...

In an ecosystem various trophic levels are interlinked with each through a food chain. ( the transfer of food energy from green plants through a series of organism with repeated ea

What is thyroid disease, Q. What is thyroid disease? Thyroid disease: T...

Q. What is thyroid disease? Thyroid disease: Thyroid disease is associated with angina. Anginas pectoris presents itself in the form of speific symptoms which tend to re- oc

Define use of isotopically labelled nutrients, Define Use of Isotopically L...

Define Use of Isotopically Labelled Nutrients? Nutrient Turnover Radioactive labelled nutrients are used to know the total body pool and the compartment in which it is stored.

Arthropod classes characterized by number of limbs, Q. How are the three ma...

Q. How are the three major arthropod classes characterized according to the number of limbs? Most crustaceans have five pairs of limbs. Arachnids present four pairs and Insects

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd