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A cell frequently wants to secrete larger molecules than can be accommodated through the transport systems dealt with in Topic E3. Exocytoses get to the movement of proteins out of the cell across the plasma membrane into the extracellular space. The Proteins intended to be secreted from the cell are interpreted on ribosome's attached to the RER. Membrane-bound vesicles having these proteins then bud off from the RER, migrate by the cytosol and fuse with the membrane of the Golgi apparatus that is given in the figure. On transport by the endoplasmic Golgi and reticulum apparatus, various post- translational modifications to the proteins take place, like as glycosylation.
figure: Exocytosis of proteins by the constitutive and regulated secretory pathways.
Below is the N-terminus of an open reading frame of a gene that has been cloned into the E. coli expression plasmid pTrcHis. ATGTCTCTACCTCAGAGCAGGTGGGTGGCATGTTTCAGTATTGAGGGAATT
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