Define estimation of nitrogen and protein content, Biology

Assignment Help:

Estimation of Nitrogen and Protein Content Using Micro Kjeldahl's Method?

Standardization of H2SO4

Prepare a solution of 0.1 N H2SO4  by diluting 2.8 ml of the concentrated acid to 1000 ml in a volumetric flask. This is approximately 0.1N. Standardize the acid against 0.1 N Na2CO3 solution. Accurately weigh 0.530 g of anhydrous Na2CO3. Transfer to a 100 ml volumetric flask and make up the volume to the mark with distilled water.

Pipette 10 ml of this solution in a conical flask and titrate against the diluted H2SO4 prepared using 1% solution of methyl orange as an indicator. Calculate the exact normality of H2SO4 and dilute it accordingly to obtain exactly 0.0143N H2SO4.


Related Discussions:- Define estimation of nitrogen and protein content

What is a plant-incorporated protectant, What is a plant-incorporated prote...

What is a plant-incorporated protectant? Plant-incorporated protectants are pesticidal substances formed and used by the living plant, typically to protect the plant from pests

Bacterial growth medium, how a simple medium can be converted into selecti...

how a simple medium can be converted into selective differential and enriched medium?

Gumbout chiton, what is the classification of the Gumbout Chiton?

what is the classification of the Gumbout Chiton?

What do you mean by bleeding index, Q. What do you mean by Bleeding Index? ...

Q. What do you mean by Bleeding Index? This factor is applicable at this stage to evaluate the health of one stage implants in which the transmucosal component allows the forma

Define etiological risk factors in cancer, Define Etiological Risk Factors ...

Define Etiological Risk Factors in Cancer? Cancer risks are climbing because of increasingly sedentary lifestyles and diets which are high in fat and sugar although low in frui

Explain the heat fixation method, Explain the Heat Fixation Method? Thi...

Explain the Heat Fixation Method? This is the usual method. Here, bacteria is fixed by gentle heating of air-dried bacterial film, which results in coagulation of bacterial pro

Functions of the bacterial flora within the human gut, Q. What are the majo...

Q. What are the major functions of the bacterial flora within the human gut? Bacteria that live inside the gut have great importance in digestion. Several polysaccharides like

Determine the term epitheliomuscular cell, Determine the term Epitheliomusc...

Determine the term Epitheliomuscular cell. Cells which line outer surface of cnidarians. These cell have two functions: first is to form the outer body covering of animal, seco

Concentration Gradients and Membrane Permeability, 3. Which substance(s) cr...

3. Which substance(s) crossed the dialysis membrane? Support your response with data-based evidence.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd