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Estimation of Nitrogen and Protein Content Using Micro Kjeldahl's Method?
Standardization of H2SO4
Prepare a solution of 0.1 N H2SO4 by diluting 2.8 ml of the concentrated acid to 1000 ml in a volumetric flask. This is approximately 0.1N. Standardize the acid against 0.1 N Na2CO3 solution. Accurately weigh 0.530 g of anhydrous Na2CO3. Transfer to a 100 ml volumetric flask and make up the volume to the mark with distilled water.
Pipette 10 ml of this solution in a conical flask and titrate against the diluted H2SO4 prepared using 1% solution of methyl orange as an indicator. Calculate the exact normality of H2SO4 and dilute it accordingly to obtain exactly 0.0143N H2SO4.
how do they adapt to their parasitic mode of feeding
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