Define estimation of nitrogen and protein content, Biology

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Estimation of Nitrogen and Protein Content Using Micro Kjeldahl's Method?

Standardization of H2SO4

Prepare a solution of 0.1 N H2SO4  by diluting 2.8 ml of the concentrated acid to 1000 ml in a volumetric flask. This is approximately 0.1N. Standardize the acid against 0.1 N Na2CO3 solution. Accurately weigh 0.530 g of anhydrous Na2CO3. Transfer to a 100 ml volumetric flask and make up the volume to the mark with distilled water.

Pipette 10 ml of this solution in a conical flask and titrate against the diluted H2SO4 prepared using 1% solution of methyl orange as an indicator. Calculate the exact normality of H2SO4 and dilute it accordingly to obtain exactly 0.0143N H2SO4.


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