Define essential parts of photocolorimeter - monochromators, Biology

Assignment Help:

Define Essential Parts of Photocolorimeter - Monochromators?

This is a means of selecting a sufficiently narrow waveband. Early colorimeters used glass filters that transmitted a wide segment of the spectrum (50 nm or more). Newer instruments use interference filters that consist of thin layer of magnesium fluoride crystals with a semitransparent coating of silver on each side. Interference filters have a band pass of 5-8 nm. The term band pass is defined as the width of the spectrum that will be isolated by a monochromator, i.e. it is the range of wavelength between the points at which the transmittance is equal to one half of the peak transmittance.

The inexpensive spectrophotometers commonly used in the laboratory have a band pass of 20 nm but more sophisticated instruments may have a band pass of 0.5 nm. The monochromator consists of an entrance slit to exclude unwanted or stray light followed by absorption or interference filters, prisms or diffraction grating for wavelength selection. This is preceded by a series of light focussing lenses. Instruments using filters as wavelength selector requires lenses to focus correctly the light from the source through the filter and cuvette to the detector. But in the ultraviolet range, quartz or fused silica is essential because glass does not transmit light efficiently at wavelength shorter than 340 nm. An exit slit at the end of monochromator allows only a narrow fraction of the spectrum to reach the sample cuvette. Some spectrophotometers have a nonmovable slit whereas in some the slit width varies automatically as the wavelength changes.


Related Discussions:- Define essential parts of photocolorimeter - monochromators

Genetic reservoir - conservation of wildlife, Genetic Reservoir - Conservat...

Genetic Reservoir - Conservation of Wildlife  Despite the present and future economic and health importance to human beings, very little is known about most of the earth's 1.

Conduits-surgery for coronary artery disease, Conduits Figure: Ve...

Conduits Figure: Venous Conduits Reversed saphenous vein was the first conduit used for CABG. 'It is usually harvested from the leg starting above the ankle going up

Zoonoses disease- histoplamosis, Histoplamosis Caveran disease, darling’s ...

Histoplamosis Caveran disease, darling’s disease, reticuloendothelial cytomycosis or tingo maria fever are the other synonyms for histoplamosis caused by Histoplasma capsulatum. T

Phylum mollusca, PHYLUM  MOLLUSCA Definition  and Introduction B...

PHYLUM  MOLLUSCA Definition  and Introduction Basically bilateral and protostomial eucoelomate  eumetazoans  whose soft body (L. Millis  or molluscum= soft) is unsegmente

Define about the photometry - colorimetry, Define about the Photometry - co...

Define about the Photometry - colorimetry? Photometry is the measurement of the luminous intensity light or the amount of luminous light falling on a surface from such a source

Define key concepts and facts of polysaccharides, Define Key Concepts and F...

Define Key Concepts and Facts of Polysaccharides? 1. All polysaccharides contain several monosaccharide units. 2. All monosaccharide units are joined to each other by glycosidi

Enumerate the term diagnosis of epilepsy, State the diagnosis of epilepsy ...

State the diagnosis of epilepsy A diagnosis of epilepsy is usually confirmed by an EEG. In some epileptics, however, seizures are difficult to demonstrate in this way, except u

Chronic mitral regurgitation-indications for surgery, Chronic Mitral Regurg...

Chronic Mitral Regurgitation :  In chronic mitral regurgitation, the asymptomatic phase is much longer than in mitral stenosis. Onset of symptoms may also mean the onset of l

What is the significance of immunogenicity, Problem: Explain the proced...

Problem: Explain the procedure for import of cosmetics in US. - Briefly explain the procedure for import of cosmetics in US What are the major points to be considered whe

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd