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Define Eosin - Methylene Blue (EMB) Agar (Levine)
Peptone - 10.0 gm
Lactose - 5.0 gm
Dipotassium - 2.0 gm
Hydrogen Phosphate
Agar - 13.5 gm
Eosin Y - 0.4 gm
Methylene blue - 0.065 gm
Distilled water - 1000 ml
pH - 7.2
Dissolve the ingredients and autoclave at 15 psi before dispensing it into sterile plates and tubes. The medium is used for distinguishing lactose fermenting gram negatives from non-lactose fermenting types.
Explain Precautions for preparation of general purpose media 1. Dissolve ingredients one by one in distilled water. 2. Check and set the medium to appropriate pH. 3. Auto
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