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Define Eosin - Methylene Blue (EMB) Agar (Levine)
Peptone - 10.0 gm
Lactose - 5.0 gm
Dipotassium - 2.0 gm
Hydrogen Phosphate
Agar - 13.5 gm
Eosin Y - 0.4 gm
Methylene blue - 0.065 gm
Distilled water - 1000 ml
pH - 7.2
Dissolve the ingredients and autoclave at 15 psi before dispensing it into sterile plates and tubes. The medium is used for distinguishing lactose fermenting gram negatives from non-lactose fermenting types.
At 1 AM, an impermeable membrane separates a 1 liter solution of 1M NaCl in the left compartment from a 1 liter solution containing both 1M NaCl and 1M KCl in the right compartment
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