Define eosin - methylene blue (emb) agar (levine), Biology

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Define Eosin - Methylene Blue (EMB) Agar (Levine)

Peptone - 10.0 gm

Lactose - 5.0 gm

Dipotassium - 2.0 gm

Hydrogen Phosphate

Agar - 13.5 gm

Eosin Y - 0.4 gm

Methylene blue - 0.065 gm

Distilled water - 1000 ml

pH - 7.2

Dissolve the ingredients and autoclave at 15 psi before dispensing it into sterile plates and tubes. The medium is used for distinguishing lactose fermenting gram negatives from non-lactose fermenting types.


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