Define disadvantages of direct microscopic count, Biology

Assignment Help:

Define Disadvantages of Direct Microscopic Count?

1. Small cells are difficult to see under the microscope and may be missed.

2. It gives total count, i.e., both live and dead cells as it is difficult to distinguish between the two.

3.High concentration of microbes, i.e., about 10 million bacteria/ml is required as very small volume of microbes is sampled.The method is not sensitive to populations of fewer than 1 million.

4. Motile cells must be immobilized before counting.

5. Phase contrast microscope is needed for unstained cells.


Related Discussions:- Define disadvantages of direct microscopic count

Genital warts and human papillomavirus infection, Genital warts and human p...

Genital warts and human papillomavirus (hpv) infection External genital warts are caused by human papillo- mavirus, usually type 6 or 11; other types (16, 18 and others) cause

How different is the amphibian heart from the fish heart, Q How different i...

Q How different is the amphibian heart from the fish heart? The fish heart has only two chambers, a ventricle and an atrium, and the blood that comes to it is purely venous.

What is the difference among living and non-living things, What is the diff...

What is the difference among living and non-living things? Living things obtain and use energy. They also grow throughout their lifetime. Living this is made of cells. Living t

Carbohydrates, Carbohydrates Fifty five to seventy per cent of the re...

Carbohydrates Fifty five to seventy per cent of the required energy in animals is derived from carbohydrates. However, fats and proteins can also be broken down and used for

Explain elastic fibers would not wrinkle, As we age, our skin wrinkles due ...

As we age, our skin wrinkles due to change in collagen and elastic fibers in the dermis and a decrease in their production. Explain why topical application of collagen and elastic

Explain the Synthetic and natural materials of suturing, Explain the Synthe...

Explain the Synthetic and natural materials of suturing 1. Most common are synthetic fibers: nylon, polyester, polyglactin (PG), and polyglycolic acid (PGA). 2. Natural: sil

Listeriosis, Listeriosis The organisms have an extensive host range which ...

Listeriosis The organisms have an extensive host range which includes mammal, poultry, fish, crustaceans and ticks including man. The organism has been isolated from sheep, goats,

What is the structural formula of glycerol, What is the structural formula ...

What is the structural formula of glycerol? To which organic function do these molecules belong? Glycerol is a linear chain of 3 carbons; the central carbon is bound to one hyd

Malignant catarrhal fever (mcf), Malignant catarrhal fever (MCF) Malig...

Malignant catarrhal fever (MCF) Malignant catarrhal fever is invariably fatal generalized lymphoproliferative disease of cattle and sometimes of wild ruminants. It is common i

Necrotic enteritis, N e c r otic enteritis It can be designated as ...

N e c r otic enteritis It can be designated as enterotoxaemia of chickens, turkeys and ducks caused by Clostridium perfringens. The toxin released by this spore-bearing a

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd