Define ascending type of paper chromatography, Biology

Assignment Help:

Define ascending type of paper chromatography?

The ascending type consists in dipping the lower end of the paper containing the sample spots into the solvent so that it is above the solvent depth and then allowing it to rise up the paper by capillary action. The mobile phase or solvent moves in the upward direction against the gravitational force. The rate of movement of solvent becomes slower with increasing height which provides enough time for partition equilibrium between the two phases to take place. However the disadvantage in this is that compounds with low Rf values may not separate well.


Related Discussions:- Define ascending type of paper chromatography

Define about the sporocyst larva, Define about the Sporocyst Larva? Sp...

Define about the Sporocyst Larva? Sporocyst is the second larval stage in the life cycle of F. hepatica. It develops from the miracidium larva within the pulmonary chamber of

Vitamin - k, VITAMIN - K Also known as naptho quionone / anti haemo...

VITAMIN - K Also known as naptho quionone / anti haemorrhagic vitamin / coagulation vitamin / menadone . It requires for formation of blood clotting factor prothrombin.

What happens within the cell in the second interface period, What are the e...

What are the events that mark the beginning and the end of the second interphase period? What happens in the cell in this period? The second interphase period is the S. It begi

Evaluate the net energetic gain of the process, Q. How many ATP molecules a...

Q. How many ATP molecules are made after the aerobic respiration and what is the net energetic gain of the process? After aerobic respiration 38 ATP molecules are made with the

Describe the terms diffusion osmosis and filtration, Describe the terms dif...

Describe the terms diffusion, osmosis and filtration. Explain why each process is important for the human body.

Objectives of surgery, OBJECTIVES :  After reading surgery, you will be ab...

OBJECTIVES :  After reading surgery, you will be able to: 1 understand the fundamental of closed and open heart surgery and recent developments in off pump surgery; 2 in

Explain working of cerebral cortex, Q. Explain working of Cerebral Cortex? ...

Q. Explain working of Cerebral Cortex? Cerebral Cortex/Cerebrum: The cerebrum or the cortex is the large part of the brain and is associated with the cognitive functions of the

Why the ph of urine can be quite variable, The ph of urine can be quite var...

The ph of urine can be quite variable. Why? Why is bromothymol blue used as a ph indicator instead of phenal red when titrating blood?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd