Define ascending type of paper chromatography, Biology

Assignment Help:

Define ascending type of paper chromatography?

The ascending type consists in dipping the lower end of the paper containing the sample spots into the solvent so that it is above the solvent depth and then allowing it to rise up the paper by capillary action. The mobile phase or solvent moves in the upward direction against the gravitational force. The rate of movement of solvent becomes slower with increasing height which provides enough time for partition equilibrium between the two phases to take place. However the disadvantage in this is that compounds with low Rf values may not separate well.


Related Discussions:- Define ascending type of paper chromatography

Metallurgical coke, Metallurgical coke should have following characteristic...

Metallurgical coke should have following characteristics: 1.      Purity: low amounts of moisture, ash, sulphur & phosphorous should be present. 2.      Porosity: it should b

Active or moderately active lifestyles - physical activity, Define Active o...

Define Active or moderately active Lifestyles - physical activity? These people have occupations that p-e not strenuous in terms of energy demands, but involve more energy expe

Protein of animal origin - meat, Protein of Animal Origin - Meat Meat:...

Protein of Animal Origin - Meat Meat: Skeletal or striated muscles are used for food purposes. Flesh of cattle, sheep and swine comprise most of the meat contents. Edible mea

Explain characteristics that make breastfeeding beneficial, Explain charact...

Explain characteristics that make breastfeeding beneficial to the infant? We have listed some characteristics that make breastfeeding beneficial to the infant: • Mature milk

What is an analogy for microtubules, What is an analogy for microtubules? ...

What is an analogy for microtubules? Microtubules have two major functions in cells and in doing so act such as a skeleton as well as like railroad tracks. Microtubules are the

A monohybrid cross, A monohybrid cross: A.Determines the genetic makeup of ...

A monohybrid cross: A.Determines the genetic makeup of an organism B.always involves homozygous alleles. C.always involves organisms that are heterozygous at all loci. D.Always inv

Prozoan, classifcation of protozoans

classifcation of protozoans

Explain Objectives of root-end resection apicoectomy, Explain Objectives of...

Explain Objectives of Root-End Resection Apicoectomy a) Removal of untreated apical portion of the root, b) Enable the obturator to determine the cause of failure,  c) To

How have prokaryotic cells give origin to aerobic eukaryotic, How have prok...

How have prokaryotic cells given origin to aerobic eukaryotic cells and to photosynthetic aerobic eukaryotic cells? As per to the most accepted hypothesis aerobic eukaryotic ce

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd