Define adsorption chromatography -basic separation technique, Biology

Assignment Help:

Define Adsorption chromatography - Basic Separation Technique?

Adsorption is surface interaction and is by number of forces, between surface atoms of solids and molecules of external solute. Adsorption chromatography comprises of systems where active absorption occurs at the surface of the solid stationary medium, often alumina or silica gel. Competition for adsorptive sites occurs between the molecules of the mixture to be separated and the molecules of mobile phase, be it gas or liquid.

The speed of migration of a component will depend on its adsorptive affinity relative to the other species present. A number of attractive forces (Vander Waal forces, hydrogen bonding etc.) between the stationary phase and substances to be separated have been recognized and separation takes place due to differences in these adsorption forces.


Related Discussions:- Define adsorption chromatography -basic separation technique

How are the beings of the phylum annelida classified, Q. Concerning the occ...

Q. Concerning the occurrence of separated sexes how are the beings of the phylum Annelida classified? These beings may be dioecious (the majority of polychaetes) or hermaphrodi

What were genotypes of the f2 plants crosses, A snapdragon plant that bred ...

A snapdragon plant that bred true for white petals was crossed to a plant that bred for purple petals, and all the F1 had white petals. The F1 was selfed. Among the F2, three other

Explain the nucleic acid is the transforming agent or not, Fredrick Griffit...

Fredrick Griffith published a series of experiments that collectively were dubbed "The Transforming Principle" in 1928. Which of the following prevented him from being able to decl

Pulses - hypertension, Pulses Careful examination of both upper and lo...

Pulses Careful examination of both upper and lower limb pulses is useful in detecting coarctation, and other arterial stenosis. The carotid arteries should be checked for sten

MACRO TAXONOMY, WHAT IS CHARACTER SELECTION CRITERI

WHAT IS CHARACTER SELECTION CRITERIA

Procedures for diagnosis - demonstration of specific antigen, Demonstration...

Demonstration of specific antigen(s): The presence of the viral antigen in the infected tissues and cells can be demonstrated by fluorescent antibody technique (FAT) and

Explain about precautionary principle, Q. Explain about precautionary princ...

Q. Explain about precautionary principle? The ethical values of biodiversity highlights the intrinsic value of biodiversity for its own sake and it is independent of the varied

What is arterial switch operation explain briefly, What is arterial switch ...

What is arterial switch operation explain briefly? Arterial Switch Operation: Switching the Great arteries to restore the normal Ventriculoarterial connections. The coronary

Pvcs during recovery, As the heart rapidly slows during recovery from exerc...

As the heart rapidly slows during recovery from exercise, PVCs commonly occur and usually have no clinical significance. This may be a time when metabolic adjustments in the heart

Water soluble vitamins, Water Soluble Vitamins B-vitamins are abundant...

Water Soluble Vitamins B-vitamins are abundant in milk and other feeds. B-vitamins are synthesized by rumen microorganisms, beginning soon after a young animal begins feeding.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd