Define adsorption chromatography -basic separation technique, Biology

Assignment Help:

Define Adsorption chromatography - Basic Separation Technique?

Adsorption is surface interaction and is by number of forces, between surface atoms of solids and molecules of external solute. Adsorption chromatography comprises of systems where active absorption occurs at the surface of the solid stationary medium, often alumina or silica gel. Competition for adsorptive sites occurs between the molecules of the mixture to be separated and the molecules of mobile phase, be it gas or liquid.

The speed of migration of a component will depend on its adsorptive affinity relative to the other species present. A number of attractive forces (Vander Waal forces, hydrogen bonding etc.) between the stationary phase and substances to be separated have been recognized and separation takes place due to differences in these adsorption forces.


Related Discussions:- Define adsorption chromatography -basic separation technique

Describe the classification of arthropoda, Describe the Classification of A...

Describe the Classification of Arthropoda? Most arthropods also go through a growth process called metamorphosis, whereby they change their bodies into an entirely different fo

How can the endoskeleton of echinoderm, Q How can the endoskeleton of echin...

Q How can the endoskeleton of echinoderms be characterized in comparison to analogous structures present in vertebrates, arthropods and molluscs? The echinoderm skeleton is int

Enzymes, Biological catalysts à speed up chemical reactions in the body wit...

Biological catalysts à speed up chemical reactions in the body without being consumed in the process Reduce the activation energy needed for reactants to reach the transit

Dna replication in eukaryotes, DNA replication in eukaryotes is much more c...

DNA replication in eukaryotes is much more complex than in prokaryotes while there are various same aspects. The Eukaryotic cells can only initiate DNA replication at a particular

How do taenias obtain food and make gas exchange, Q. How do taenias obtain ...

Q. How do taenias obtain food and make gas exchange? The Tapeworms have sucking and hooks structures on their heads (scolex) that fixate the parasite in the gut wall these stru

Define some beneficial effects of fungi, Define some Beneficial Effects of ...

Define some Beneficial Effects of Fungi? 1. These act as decomposers and can degrade complex organic molecules in dead plants and animals to simple organic compounds and inorga

Gaseous exchange, Gaseous Exchange Gaseous exchange is take place on b...

Gaseous Exchange Gaseous exchange is take place on by both mantle and the gills. The gills of most bivalves are highly changed for filter feeding. They are derived from the pr

What is Inoculation and Culturing in Microbiology, What is Inoculation and ...

What is Inoculation and Culturing? In microbiology, inoculation is the process where a microbe of interest is introduced into a previously sterilized growth medium for the p

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd