Define adsorption chromatography -basic separation technique, Biology

Assignment Help:

Define Adsorption chromatography - Basic Separation Technique?

Adsorption is surface interaction and is by number of forces, between surface atoms of solids and molecules of external solute. Adsorption chromatography comprises of systems where active absorption occurs at the surface of the solid stationary medium, often alumina or silica gel. Competition for adsorptive sites occurs between the molecules of the mixture to be separated and the molecules of mobile phase, be it gas or liquid.

The speed of migration of a component will depend on its adsorptive affinity relative to the other species present. A number of attractive forces (Vander Waal forces, hydrogen bonding etc.) between the stationary phase and substances to be separated have been recognized and separation takes place due to differences in these adsorption forces.


Related Discussions:- Define adsorption chromatography -basic separation technique

What are the main causes of mutation, Q. What are the main causes of Mutati...

Q. What are the main causes of Mutation? Some mutations take place randomly and spontaneously during process of mitosis or meiosis. Others are caused by mutagenic agents that a

Economically important fermentation products-rice beer, Normal 0 ...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Function of noradrenaline in consciousness, Q. Function of Noradrenaline in...

Q. Function of Noradrenaline in consciousness? Increased level of noradrenalin is implicated in wakefulness. Locus coeruleus noradrenergic neurons decrease their rate of firing

Explain xylose isomeras, Explain xylose isomeras A mixture of glucose ...

Explain xylose isomeras A mixture of glucose and fructose, is produced from sucrose by the action of yeast invertase (β-fructofuranosidase), an enzyme which can only be extrac

Kinase, A kinase is in general an enzyme which catalyzes the transfer of th...

A kinase is in general an enzyme which catalyzes the transfer of the phosphate group from ATP to something else. In the molecular biology, it has acquired the more specific verbal

Procedures for diagnosis - restriction enzyme analysis, R e striction...

R e striction enzyme analysis: The restriction endonuclease analysis of viral DNA fragments or DNA fingerprinting discovered in 1970s has revolutionized the study o

What are the four groups of protozoans, Q. What are the four groups of prot...

Q. What are the four groups of protozoans? The four main groups of protozoans are the mastigophores flagellated, like the trypanosome that causes Chagas' disease, the sarcodine

What is the digestive enzyme that acts within the stomach, What is the dige...

What is the digestive enzyme that acts within the stomach? Which type of food does it digest? What are the cells that produce that enzyme? The digestive enzyme that acts in the

Describe about mitochondria and how they are associated with, Mitochondria,...

Mitochondria, which fuel life's processes in all cells, uses nutrients from digested food and oxygen which has diffused across cell surface. In freshwater protozoans water expulsio

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd