Define adsorption chromatography -basic separation technique, Biology

Assignment Help:

Define Adsorption chromatography - Basic Separation Technique?

Adsorption is surface interaction and is by number of forces, between surface atoms of solids and molecules of external solute. Adsorption chromatography comprises of systems where active absorption occurs at the surface of the solid stationary medium, often alumina or silica gel. Competition for adsorptive sites occurs between the molecules of the mixture to be separated and the molecules of mobile phase, be it gas or liquid.

The speed of migration of a component will depend on its adsorptive affinity relative to the other species present. A number of attractive forces (Vander Waal forces, hydrogen bonding etc.) between the stationary phase and substances to be separated have been recognized and separation takes place due to differences in these adsorption forces.


Related Discussions:- Define adsorption chromatography -basic separation technique

Heart in fishes, Fishe s - Heart is 2 chambered 1 auricle & 1 ventr...

Fishe s - Heart is 2 chambered 1 auricle & 1 ventricle present. Sinus venosus, truncus or conus arteriusus present. Only impure blood come in heart so heart is venous he

How to devise a series of dilutions, A solution was prepared by dissolving ...

A solution was prepared by dissolving 25g NaOH powder in 100 ml water. Using not more than 15 ml water, devise a series of dilutions that will give a concentration of 5mg/ml NaOH.

Where smooth muscle may be found in human body, a) Name three parts of the ...

a) Name three parts of the body where smooth (involuntary or unstriated) muscle may be found. b) In any one of your examples, say what the smooth muscle does.

Genic type-genetic basis of sex determination, Genic Type-Genetic basis of ...

Genic Type-Genetic basis of Sex Determination In some organisms, certain indepe~ident genes located on different chromosomes are responsible for sex determination. Most species

Explain biological function, Q. Concerning their biological function what i...

Q. Concerning their biological function what is the difference between meiosis and mitosis? The main biological function of mitosis is cellular multiplication a fundamental pro

Golgi apparatus, GOLGI APPARATUS Camillo Golgi in 1898 discovered a retic...

GOLGI APPARATUS Camillo Golgi in 1898 discovered a reticular structure in the cytoplasm of nerve cells with the help of metal impregnation technique using silver nitrate for whic

What is the importance of vitamin A, What is the importance of vitamin A ...

What is the importance of vitamin A The importance of vitamin A is undisputable. You may already be aware about the functions/role of vitamin A in our body.  Vitamin A is absol

Determine the cylindrical body of nematodes brought, Compared to platyhelmi...

Compared to platyhelminthes which physiological problem have the cylindrical body of nematodes brought? How was that problem solved? The cylindrical shape of nematodes made imp

Explain about diabetes mellitus, Q. Explain about Diabetes Mellitus? Di...

Q. Explain about Diabetes Mellitus? Diabetes Mellitus is a metabolic disorder characterized by chronic hyperglycemia (increased sugar) with disturbances of carbohydrates, fats

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd