Define adsorption chromatography -basic separation technique, Biology

Assignment Help:

Define Adsorption chromatography - Basic Separation Technique?

Adsorption is surface interaction and is by number of forces, between surface atoms of solids and molecules of external solute. Adsorption chromatography comprises of systems where active absorption occurs at the surface of the solid stationary medium, often alumina or silica gel. Competition for adsorptive sites occurs between the molecules of the mixture to be separated and the molecules of mobile phase, be it gas or liquid.

The speed of migration of a component will depend on its adsorptive affinity relative to the other species present. A number of attractive forces (Vander Waal forces, hydrogen bonding etc.) between the stationary phase and substances to be separated have been recognized and separation takes place due to differences in these adsorption forces.


Related Discussions:- Define adsorption chromatography -basic separation technique

Autoregulation of coronary blood flow, The ability to maintain myocardial p...

The ability to maintain myocardial perfusion at constant levels in the face of changing driving presence is termed autoregulation. In normal cases, autoregulation is maintained at

Biodiverse ecosystems of economic application, Q. What are some economic ap...

Q. What are some economic applications that can be generated by very biodiverse ecosystems? Extremely biodiverse areas present huge economic potential and they can be a source

Cost of accidents, Cost of Accidents Immediate Cost Immediate c...

Cost of Accidents Immediate Cost Immediate cost takes into account the loss of work, damages to equipment and facilities which have to repaired and restored or replaced

What are the chronic complications of diabetes, Q. What are the Chronic com...

Q. What are the Chronic complications of diabetes? • Atherosclerosis: Degeneration of walls of the arteries due to fatty plaques - deposition on arterial walls. Diabetics ar

What is the nucleolus, What is the nucleolus? The nucleolus is a small ...

What is the nucleolus? The nucleolus is a small and optically dense region in the interior of the cell nucleus. It is made of ribosomic RNA (rRNA) and proteins. Single nucleus

What is the adnexal organ of the digestive system, Q. What is the adnexal o...

Q. What is the adnexal organ of the digestive system in which bile is stored? How does this organ react to the ingestion of fat rich food? Bile is concentrated and stored in th

What are contraction of sarcomeres of the muscle cells, Q. What are the few...

Q. What are the few examples of movement created by the contraction of sarcomeres of the muscle cells? The handling of a cup of coffee, the peristaltic movements of the bowels,

Explain the determination and application of vitamin e, Explain the Determi...

Explain the Determination of Vitamin E DL-α-Tocopherol is oxidized with ferric chloride and the ferrous ions formed are measured colorimetrially with the aid of α,α'-dipyridyl

What can be deduced about the genotype, In pet rabbits, brown coat colour i...

In pet rabbits, brown coat colour is recessive to black coat colour. A black female rabbit gives birth to four black-coated and three brown-coated baby rabbits. What can be deduced

Standard titration - estimation of vitamin c in chillies, Define Standard t...

Define Standard titration - estimation of vitamin c in Chillies? Pipette 5 ml of standard ascorbic acid solution into a 100 ml conical flask. Fill the burette with the dye solu

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd