Define adsorption chromatography -basic separation technique, Biology

Assignment Help:

Define Adsorption chromatography - Basic Separation Technique?

Adsorption is surface interaction and is by number of forces, between surface atoms of solids and molecules of external solute. Adsorption chromatography comprises of systems where active absorption occurs at the surface of the solid stationary medium, often alumina or silica gel. Competition for adsorptive sites occurs between the molecules of the mixture to be separated and the molecules of mobile phase, be it gas or liquid.

The speed of migration of a component will depend on its adsorptive affinity relative to the other species present. A number of attractive forces (Vander Waal forces, hydrogen bonding etc.) between the stationary phase and substances to be separated have been recognized and separation takes place due to differences in these adsorption forces.


Related Discussions:- Define adsorption chromatography -basic separation technique

Phases of seed formation, Phases of Seed Formation The process of seed...

Phases of Seed Formation The process of seed formation can be divided into two phases: The division phase, in which fertilised egg divides mitotically and complet

Insulin dependent diabetes mellitus, Q. What is Insulin Dependent Diabetes ...

Q. What is Insulin Dependent Diabetes Mellitus? Mostly children and adolescents suffer from this type of diabetes however; it may appear in adults and elderly too. In this, the

Why is the fish circulation classified as simple circulation, Why is the fi...

Why is the fish circulation classified as a simple and complete circulation? Complete circulation is that in which there is no mixture of venous blood and arterial blood. As ci

Explain minerals requirement during thyphoid, Explain Minerals requirement ...

Explain Minerals requirement during thyphoid Minerals:   Loss of electrolytes and water is observed due to diarrhoea. Thus liberalizing on sodium intake  through salty soups,

Explain the mechanism of temperature regulation, Any significant variation ...

Any significant variation in the internal temperature could have damaging effects on the body's enzymes. Homeotherms or warm-blooded animals including human can however generate co

What are ions, What are ions? What are the two types of molecules into whic...

What are ions? What are the two types of molecules into which ions are classified? Ions are atoms or substances electrically charged by means of loss or gain of electrons.

Advantage & disadvantage of using fungi as source of protein, Define Advant...

Define Advantage & disadvantage of using fungi as source of protein? Advantages 1. Easy to harvest from culture medium. 2. Texture of the fungi improves the functional

Characteristics of river systems, Characteristics of River Systems Th...

Characteristics of River Systems The basic function of the rivers is to convey surplus rain water from land to sea. Annually the rivers carry fresh water, equivalent to 25 cm

Abrasive action of water and wind, Abrasive action of water, ice and wind ...

Abrasive action of water, ice and wind Water is an important mechanical weathering agent both in liquid as well as in the frozen state.  Rain water carrying sediments, ice glac

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd