Define adsorption chromatography -basic separation technique, Biology

Assignment Help:

Define Adsorption chromatography - Basic Separation Technique?

Adsorption is surface interaction and is by number of forces, between surface atoms of solids and molecules of external solute. Adsorption chromatography comprises of systems where active absorption occurs at the surface of the solid stationary medium, often alumina or silica gel. Competition for adsorptive sites occurs between the molecules of the mixture to be separated and the molecules of mobile phase, be it gas or liquid.

The speed of migration of a component will depend on its adsorptive affinity relative to the other species present. A number of attractive forces (Vander Waal forces, hydrogen bonding etc.) between the stationary phase and substances to be separated have been recognized and separation takes place due to differences in these adsorption forces.


Related Discussions:- Define adsorption chromatography -basic separation technique

Fensom and spanner electroosmotic flow hypothesis, Fensom and Spanner Elect...

Fensom and Spanner Electroosmotic Flow Hypothesis In electroosmosis the ions flow across a membrane in response to electrical gradient. Ions pull along water and other content

What are the hormones secreted by the adrenal cortex, Q. What are the hormo...

Q. What are the hormones secreted by the adrenal cortex? What are their respective functions? The cortical portion of the adrenals secretes hormones of the corticoid (or cortic

Eye colour, what factor''s do i colour depend on?

what factor''s do i colour depend on?

Healthcare delivery - risk pooling, Normal 0 false false fa...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Explain mendels law of dominance, Which one of the following cannot be expl...

Which one of the following cannot be explained on the basis of Mendel's Law of Dominance? 1. The discrete unit controlling a particular character is called a factor 2. Out o

Avian leukosis (sarcoma group of retroviruses), A v i a n leukosis (Sar...

A v i a n leukosis (Sarcoma group of retroviruses) This is a complex of viral diseases caused by an avian retrovirus with various manifestations such as lymphoid leukosis,

Choice of graft material depends on, Choice of graft material depends on ...

Choice of graft material depends on - Its nature towards bone e.g., Osteoconductive or osteoinductive - Its architecture i.e., granular or block, pore si-e - Its origin -

Name the foods to avoid in soft diet, Name the Foods to avoid  in soft die...

Name the Foods to avoid  in soft diet include: Fried foods and nuts, rich pastries and desserts, Raw  vegetables, Heavily spiced foods, Gas-forming vegetables, S

Explain hydrolyases, Hydrolyases These enzymes (code EC 3) catalyze th...

Hydrolyases These enzymes (code EC 3) catalyze the hydrolytic cleavage of C-O, C-N, C-C and some other bonds, including phosphoric anhydride bonds.  Their trivial names  are f

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd