Define adsorption chromatography -basic separation technique, Biology

Assignment Help:

Define Adsorption chromatography - Basic Separation Technique?

Adsorption is surface interaction and is by number of forces, between surface atoms of solids and molecules of external solute. Adsorption chromatography comprises of systems where active absorption occurs at the surface of the solid stationary medium, often alumina or silica gel. Competition for adsorptive sites occurs between the molecules of the mixture to be separated and the molecules of mobile phase, be it gas or liquid.

The speed of migration of a component will depend on its adsorptive affinity relative to the other species present. A number of attractive forces (Vander Waal forces, hydrogen bonding etc.) between the stationary phase and substances to be separated have been recognized and separation takes place due to differences in these adsorption forces.


Related Discussions:- Define adsorption chromatography -basic separation technique

Define enrichment media - culture media, Define Enrichment Media - Culture ...

Define Enrichment Media - Culture Media? It contains selective ingredients in the medium, which shifts the growth in a mixed microbial population to a particular group of micro

Thyroid-stimulating hormone blood level in hypothyroidism, Q. What happens ...

Q. What happens to the TSH (thyroid-stimulating hormone) blood level in hypothyroidism? Why is there enlargement of the thyroid in the endemic goiter disease? When there is low

Mechanism of enzyme action, Mechanism  of Enzyme Action  The action of...

Mechanism  of Enzyme Action  The action of enzymes is to lower  the activation energy  or threshold  of their substrates which. Therefore  become  activated and   react  with

How many types of gametes can be formed, How many different types of gamete...

How many different types of gametes can be formed by individuals of the following genotypes? What are they in each case a) AaBb b) AaBB c) AaBbCc d) AaBBCc e) AaBbcc f) AaBbCcDdEe

Procedure for spore staining in a given bacterial culture, Explain Procedur...

Explain Procedure for Spore Staining in a Given Bacterial Culture Carry out the exercise following the steps enumerated herewith 1. Take a clean, non-greasy slide. Prepare b

Explain the the methods of community and population, What is a community? ...

What is a community? What is the difference among the methods of community and population? A community is a set of populations of living beings that live in the similar region

Interact with parent and child to exchange information , Interact with the ...

Interact with the Parent and Child to Exchange Information   At this time the nurse can learn why the child has come to the hospital and the concerns and expectations he and hi

Codon, Codon is the group of three consecutive nucleotides within the mess...

Codon is the group of three consecutive nucleotides within the messenger RNA (mRNA) which encodes a message to initiate translation, to incorporate the specific amino acid into th

Define keratomalacia - micronutrient deficiencies, Define Keratomalacia - M...

Define Keratomalacia - Micronutrient Deficiencies? This is a condition of rapid destruction and liquefaction of full thickness of cornea, leading lo prolapse of iris, resulting

Define absorption, Define Absorption, Storage and Elimination of Pyridoxine...

Define Absorption, Storage and Elimination of Pyridoxine? Pyridoxine, pyridoxal and pyridoxamine (along with their phosphorylated forms) occur in plant and animal foods. The ph

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd