Define adsorption chromatography -basic separation technique, Biology

Assignment Help:

Define Adsorption chromatography - Basic Separation Technique?

Adsorption is surface interaction and is by number of forces, between surface atoms of solids and molecules of external solute. Adsorption chromatography comprises of systems where active absorption occurs at the surface of the solid stationary medium, often alumina or silica gel. Competition for adsorptive sites occurs between the molecules of the mixture to be separated and the molecules of mobile phase, be it gas or liquid.

The speed of migration of a component will depend on its adsorptive affinity relative to the other species present. A number of attractive forces (Vander Waal forces, hydrogen bonding etc.) between the stationary phase and substances to be separated have been recognized and separation takes place due to differences in these adsorption forces.


Related Discussions:- Define adsorption chromatography -basic separation technique

Glycogen, G L YCOGEN Polymer of a-D-glucose. It has 5000-30000 uni...

G L YCOGEN Polymer of a-D-glucose. It has 5000-30000 units of a-D-glucose. Glycogen is glucosan homopolysaccharide which is the major reserve food of animals, fungi and

Bluetongue, B l u e t on g u e Bluetongue (BT) is a non-contagi...

B l u e t on g u e Bluetongue (BT) is a non-contagious and arboviral disease of both domestic and wild ruminants. The disease is enzootic in areas where reservoirs (cat

Properties of fatty acid, A fatty acid having of a hydrocarbon chain and a ...

A fatty acid having of a hydrocarbon chain and a terminal carboxylic acid group that is shown in the figure.  Most  fatty  acids  are  in  biology  have  an  even  number  of carbo

What is the treatment process of mitral stenosis, Q. What is the treatment ...

Q. What is the treatment process of mitral stenosis? Presence of symptoms or any complications is an indication for treatment. Apart from penicillin prophylaxis no other treatm

The population thus contains secretors, The A and B antigens in humans may ...

The A and B antigens in humans may be found in water-soluble form in secretions, including saliva, of some individuals (Se/Se and Se/se) but not in others (se/se). The population t

Adaptatin, what are the adaptations of amoeba

what are the adaptations of amoeba

Contrast the reproduction of bacteria with that of frogs, Contrast the repr...

Contrast the reproduction of bacteria with that of frogs. Bacteria replicate asexually by splitting in two. Frogs reproduce sexually by producing sperm and eggs. One sperm and

Outbreak of staphylococcal food poisoning, Q. What are the conditions favou...

Q. What are the conditions favouring the outbreak of Staphylococcal food poisoning? • Botulism is caused by the bacteria Clostridium botulinum. • All low acid foods can s

Cell surface receptors, Hydrophilic and some lipophilic hormones bind to ce...

Hydrophilic and some lipophilic hormones bind to cell surface receptors. These are necessary  membrane  proteins  located  in the plasma  membrane  which  bind  the  signaling  mol

Define the term - cholera, Define the term - Cholera Cholera is caused...

Define the term - Cholera Cholera is caused by the gram negative,  V. cholerae, which is acquired by ingesting food or water contaminated  by faecal material from patients or

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd