Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
Define the Flavonoids? Flavonoids constitute the largest group of plan1 polyphenols, generally arc the compounds of low molecular weight, bound to sugar molecules. You may be f
prove oxygen is produced during photosynthesis in the presence of light
A v i a n tuberculosis Tuberculosis in poultry has not remained a big threat in list of diseases due to commercialization of poultry business. It is a contagious chronic di
Dextrins Dextrins are also polymers of D-glucose held by a(l,4) glycosidic linkages. Dextrins are, in fact, formed due to partial hydrolysis of starch by enzymes such as sa
Phagocytosis is the ingestion of huge particles like as bacteria and cell debris by large endocytic vesicles called as phagosomes. In order to be ingested the particle must first
Abrasive action of water, ice and wind Water is an important mechanical weathering agent both in liquid as well as in the frozen state. Rain water carrying sediments, ice glac
Explain Syndrome X as a Effect of Obesity? People with intra-abdominal obesity with high waist- to- hip ratio are more prone to develop the metabolic syndrome X. This is charac
Define Tests to Measure Flexibility Component in the Humans? The flexibility component measures the elasticity or range of motion around a joint. This component of physical fi
how temperature acts as limiting factor explain?
What are the excretory organs in protozoa.
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd