Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
Antibacterial activity Rifaximin reaches high concentrations in the intestinal tract and is active in vitro against a broad range of enteropathogens, including ETEC, EAEC, Shi
Determine General Principles of Allergy Management? The four general principles of allergy management include: 1. Avoid factors that cause symptoms. 2. Use appropriate
1) a) Initially, how do skeletal muscles (the type of muscle attached to bones) make ATP? b) write the reaction for this procss (Crp +ADP => Cr + ATP) as a ''coupled'' reaction, id
Q. Illustrate Numerical Taxonomy? Taxonomy today is, in many dctails, different from what it was a generation ago. The use of computers by taxonomists has established an intere
Artificial respiration First - aid for drowning victims should aim to restore respiration as quickly as possible. First of all, nose, mouth and throat of the victim shoul
Why is pattern baldness more common in men than in women? Pattern baldness is handled by the allele B. Testosterone interacts with the heterozygous genotype (BB′) to make baldn
Mechanism of Stomatal Opening It has been known for over a century that stomata open because of reversible turgor changes in the guard cells. Stomata open when turgor in the g
You work with petunias and you have identified a single gene that causes their flowers to either be all pigmented (solid), or streaked with white. Depending on the genotype of this
Q. Accessory Food Substances or Vitamins ? Bacteria also vary in their need for vitamins or accessory growth factors. Some microorganisms are unable to synthesize some or all o
Glands A cell, tissue or organ which secretes specific chemical secretion is known as a gland. The glands develop from the epithelium tissues. The glands are classifi
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd