Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
What are the Fat Requirements of athletes? Fats are the body's other major energy source. It is a well known fact that fat is a highly concentrated source of energy
Define the term - cerebral arteriosclerosis An embolism can be a blood clot, a bubble of air, a deposit of oil or fat, or a small mass of cells detached from a tumor. Reduction
Q. What are the Signs used in acute pericarditis? • Pericardial friction rub is pathognomonic of pericarditis. It is heard as a phasic scatching sound. It may vary with phases
Black disease It is a fatal peracute disease caused by Clostridium novyi Type B. It is associated with liver damage caused by liver fluke. The onset of the disease is rapid fo
Q. Can you explain Toxic alkaloids? Alkaloids are the nitrogen heterocycles which occur mainly m plants as their salts of common carboxylic aciak such as citric, lac
Why is the carbon dioxide concentration a limiting factor of the photosynthesis process? When the carbon dioxide concentration is increased indefinitely is photosynthesis also incr
OBJECTION OF CELL THEORY (REASONS OF DISCARTION) (i) Living organism are composed of various types of cells and their product but members of monera & protista don't have
Changes in the conformation of molecules - Qualitative Changes You may recall that linear chain of amino acids of a protein folds into a characteristic structure. Acidic resid
Ground Water Ground water represents a major subterranean reservoir of fresh water. In general. it is not tapped by plants as it is too deep and does not suffer from loss due t
nutrition requirement for protoa
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd