Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
Q. What are the terms used in sterilization? Sterilization: It is defined as freeing the object or substance from all life of any kind. It is defined alternatively as the
A pp l i c a tion s of transgenic animals Current applications of gene transfer in farm animals include the improvement of product quality and quantity, disease resistan
Viriods and Prions Viroids In 1971, T.O.Diener reported that the spinde tuber disease of potato causing gnarling and cracking of the tuber, is caused by multiplica
Q. Silent Myocardial Ischaemia? Ans. This deals primarily with those who have never had symptoms recognized as being of cardiac origin. Others, who have had recognized myo
COMMON DISEASE OF SHEEP - 1 . ANTHRAX - Persons working in wool factories may get infection by breathing. 2 . BLACK QURTER 3 . HAEMORRHAGIC S
Skeleton - Animal Kingdom You all know that the skeleton forms the framework of the body of different organisms in the animal kingdom. Besides giving shape to the animal, it a
Categories of Gifted Species This group of nitrogen fixers has been divided into three categories: Rhizobium which includes fast growing species, Bradyrhizobium which
What is the type of cell division that allows sexual reproduction? What is gametogenesis? Meiosis is the type of cell division that permits sexual reproduction since it decreas
what is the warmest and coldest parts of the boy
about apiculture
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd