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Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
Explain the main function of the digestive system The major function of the digestive system is to ingest the food materials, digest it to absorbable end products, absorb the
theory of scientific naming system
CONTRACTI L E CELLS In addition to above mentioned three types of muscular tissue, there are contractile cells. These are as follows. 1. Myoepithelial Cells (= M
Determine the term - Akinetic and Myoclonic Seizures Akinetic seizures are ordinarily seen only in children. Usually, an affected child collapses suddenly and without warning.
Fatty acid breakdown brings about the oxidation of long-chain fatty acids. The fatty acids are first transformed to their acyl CoA (coenzyme A) derivatives and then degraded by the
GRAFFIAN FOLLICLE - It appears as knob or stigma in medulla. It is round, discovered by Graff. It is covered by 2 layers - thecae externa & theca interna. From theca i
Q. Define Triglycerides? Triglycerides are produced by the esterification of glycerol with three fatty acid molecules. They are the body's major energy store, particularly in a
Glycolysis generates two ATPs net per glucose whereas gluconeogenesis uses four ATPs and two GTPs per glucose. Thus, if both glycolysis and gluconeogenesis were allows to opera
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what are the modes of reproduction?
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