Define about the column chromatography, Biology

Assignment Help:

Define about the Column chromatography?

In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.

The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.


Related Discussions:- Define about the column chromatography

Epithelial cells of the kidney proximal tubule, Which of the following is t...

Which of the following is true for the epithelial cells of the kidney proximal tubule? A. The sodium-glucose co-transporter in the luminal membrane is responsible for the net f

Write out electronic configuration, Give number of valence electrons for te...

Give number of valence electrons for technetium. Write out electronic configuration for the valence electrons. Give the number of valence electrons and the number of electronic con

Define the diseses shigellosis, Define the diseses Shigellosis Shigello...

Define the diseses Shigellosis Shigellosis or bacterial dysentery is caused by facultative anaerobic, gram-negative, non-spore forming, rod-shaped  Shigella in the family enter

Importance value index (ivi) - synthetic characters, Importance Value Index...

Importance Value Index (IVI) - Synthetic Characters In any study of community, the quantitative value of each of the frequency density, and cover has its own importance. But t

What are vectors of parasites, Q. What are vectors of parasites? The Ve...

Q. What are vectors of parasites? The Vectors of a parasite are organisms can transport the parasite during stages of its life cycle mediating the infection of other hosts. For

After the blastula stage what is the next stage, Q. After the blastula stag...

Q. After the blastula stage what is the following stage of the embryonic development? What is the passage from blastula to the next stage called? The blastula turns into gastru

Define influence of gardening on the development of botany, Define Influenc...

Define Influence of Gardening on the Development of Botany? The influence of gardening on the development of botany may be seen in the work of John Gerard (1545-1612). Gerard p

Structure of the pancreas, Describe the structure of the pancreas. How is t...

Describe the structure of the pancreas. How is the pancreas modified to carry out its various functions? How does the human pancreas compare to that of the fetal pig?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd