Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
Q. Energy requirements to get ideal body weight? Calories: The energy requirements of adult patients is governed by their present body weight and the need to maintain a desira
Q. show the Symptoms of constipation? Have you ever suffered from constipation? Do you recall the symptoms associated with the problem? Yes, the symptoms were specific to havin
Describe the term Humidification Dry gases result in the loss of ciliary activity of the mucosa of the airway and can lead to mucosal damage as well as causing the secretions
Q. What is an example of positive feedback of the homeostatic regulation? In positive feedback the effect caused by an action stimulates the action even more. This is a rarer m
PRINCIPLES OF DARWINISM - 1 . OVERPRODUCTION (PRODIGALITY) - All organisms have enormous fertility and they multiply in geometric ratio. Examples of over product
Q. Which Foods are used by Microorganisms for Energy? The carbohydrates, especially the sugars, are most commonly used as an energy source, but other carbon compounds can also
Morphallaxis - Regeneration This kind of regeneration takes place in plants, sponges and coelenterates such as jelly fishes and hydra. The missing parts are replaced via reorg
Q. What is the significance of mitosis for the embryonic development? Every embryo grows from a single cell that bears mitosis and generates other cells that also divide themse
Failure of implant The implant is considered a failed implant if it presents with even any one of the following features: i) Pain on function (Rule out pain due to soft tis
Cold Chain System Vaccines are sensitive to heat. On exposure to heat their potency is lost. Once a vaccine has lost its potency it cannot be regained, so it is important
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd