Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
How are grasslands classified? Grasslands might be classified into steppes and savannahs. In the steppes the prevailing vegetation is grass, as in the pampas of South America
briefly explain pinocytosis
A plant with red flowers is crossed with a white-flowered plant of the similar species. All the seeds, when grown, produce plants with red flowers. Assuming that the flower colour
How do we define an living organism
Define Absorption, Storage and Elimination of Riboflavin? Riboflavin is absorbed from the small intestine through the portal vein and is passed to all tissues via general circu
Self-Monitoring of Blood Glucose (SMBG) In SMBG, patients measure and monitor their blood sugar level by themselves and assess their condition and know the response to the trea
Define the Nutritional Assessment Tools? Malnutrition/protein energy malnutrition amongst elderly persons has been observed in various studies -be it hospitalized patients, nur
Locomotor Organelles The protozoan locomotor organelle may be Flagella, Cilia or Pseudopodia. These are of considerable value in classification of protozo
B u f fa l o- p o x The disease is caused by an orthopox virus, closely related to the vaccinia virus. It is not clear whether it should be considered
Determine the importance of Educational History- assessment A complete school history includes information regarding a child's current grade placement, any grade repetitions o
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd