Define about the column chromatography, Biology

Assignment Help:

Define about the Column chromatography?

In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.

The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.


Related Discussions:- Define about the column chromatography

Nutritional management of paediatric and geriatric problems, Define Nutriti...

Define Nutritional Management of Paediatric and Geriatric Problems? This unit focused on the paediatric and geriatric problems and their nutritional management.Initially, the c

Phases divided photosynthesis, Q. What are the phases into which photosynth...

Q. What are the phases into which photosynthesis is divided? Photosynthesis is divided into the photochemical phase or light reactions and the chemical stage.

Antioxidants and flavanoids requirement in dyslipidemia, Q. Antioxidants an...

Q. Antioxidants and Flavanoids requirement in dyslipidemia? Antioxidants and Flavanoids: You must have already read about different antioxidants present in our foods. The body

Explain exudate gums, Exudate gums Most exudates gums come from compoun...

Exudate gums Most exudates gums come from compounds  produced when the plant is wounded and these substances seal the wound. Most widely used exudate gum is 'gum Arabic' exuded

Illustrate working of mitochondria and chloroplasts, Q. How is the finding ...

Q. How is the finding of ribosomes inside mitochondria and chloroplasts explained? It is a strong hypothesis that chloroplasts and mitochondria were prokaryotes that associated

Disorders of pancreas, DISORDER S OF THE PANCREAS - (i ) Diabetes me...

DISORDER S OF THE PANCREAS - (i ) Diabetes mellitus (Hypoinsulinism) The insulin-dependent diabetes mellitus (IDDM) is caused by a failure of the Beta-cells to produc

How does the intensity of facilitated diffusion differ, Q. How does the int...

Q. How does the intensity of facilitated diffusion differ in relation to the concentration of the moved substance? What is limiting factor? Like simple diffusion facilitated di

How are inhalation and expiration carried out, Q. How are inhalation and ex...

Q. How are inhalation and expiration carried out? The diaphragm exclusive of mammals and the intercostals muscles can contract or relax varying the volume of the thorax the com

Describe what is circulatory support and inotropes, Describe what is Circul...

Describe what is Circulatory Support and Inotropes ? Colloid or Crystalloids: Hypotension after PGEl infusion is common. It is the result of relative intravascular volume deple

Pregnancy - fungal infections, Pregnancy - Fungal Infections Amphoteric...

Pregnancy - Fungal Infections Amphotericin B is the preferred treatment for deep fungal infections during pregnancy. Fluconazole is teratogenic in animals and a same pattern of

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd