Define about the column chromatography, Biology

Assignment Help:

Define about the Column chromatography?

In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.

The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.


Related Discussions:- Define about the column chromatography

What is computerised tomography, What is Computerised tomography Comput...

What is Computerised tomography Computerised tomography (CT, but also known as computerised axial tomography, or CAT) provides structural images. To generate brain scans, low l

Adrenal glands, Adrenal glands There are two adrenal glands one each...

Adrenal glands There are two adrenal glands one each on the top of each kidney. Adrenal gland has two parts, the outer cortex and inner medulla. Both cortex and medull

What are the nucleic acids, Q. What are the nucleic acids? What is the hist...

Q. What are the nucleic acids? What is the historic origin of this name? RNA and DNA, the nucleic acids, are the molecules responsible for the hereditary information that comma

Which compounds results from cyclization of glucose, Which of the following...

Which of the following compounds results from cyclization of glucose? Select one: a. alpha-D-glucopyronose b. beta-D-glucopyronose c. alpha-D-glucofuranose d. beta-D

What are the illustrations of the criminal law, What are the illustrations ...

What are the illustrations of the criminal law? Illustrations of crimes' law: Illustrations of crimes are theft, reckless or murder behaviour. The major aim of illegal

Clostridium botulinum, Clostridium botulinum is an anaerobic, Gram-positive...

Clostridium botulinum is an anaerobic, Gram-positive, spore forming, rod shaped bacteria that produces the potent neurotoxin. The organism and its spores are widely distributed in

Alkaloids, Miscellaneous antinutritional substances Alkaloids: Alkal...

Miscellaneous antinutritional substances Alkaloids: Alkaloids represent a large and structurally diverse group of compounds present within angiosperm plants; the majority of

Chlorophyll and haemoglobin, dialouge on cartoon between chlorophyll and ha...

dialouge on cartoon between chlorophyll and haemoglobin

Botany, how can i earn money by submitting assignments?

how can i earn money by submitting assignments?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd