Define about the column chromatography, Biology

Assignment Help:

Define about the Column chromatography?

In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.

The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.


Related Discussions:- Define about the column chromatography

To induce the formation of a complete plant, In the process of tissue cultu...

In the process of tissue culture in plants, what is required to induce the formation of a complete plant, in addition to a growth medium with nutrients?   The appropriate p

Porifera, what are the general characteristicss of porifera

what are the general characteristicss of porifera

Some important zoonoses-rabies , Some important zoonoses Zoonoses are ...

Some important zoonoses Zoonoses are a heterogeneous group of diseases with a variety of causative agents. On the basis of causative agents, they are grouped into bacterial, p

Define institutional and cultural barriers - infuse biology, Define Institu...

Define Institutional and Cultural Barriers - Infuse Biology? Inside the university, the departmental promotion and tenure committees understand well the value of contribution t

State the purposes of dressing, PURPOSES OF DRESSING A dressing can hav...

PURPOSES OF DRESSING A dressing can have a number of purposes, depending on the type, severity and position of the wound, although all purposes are focused towards promoting re

Feline leukemia, Feline leukemia The disease is caused by a retrovirus and...

Feline leukemia The disease is caused by a retrovirus and is the most common naturally occurring lymphosarcoma and accounts for nearly 30% of feline tumors. Based on the location

Acid rain, What direct or indirect effect is acid rain thought to have on m...

What direct or indirect effect is acid rain thought to have on mama''s and forest and buildings

Effects of environmental disasters, Primary effects 1.      Nuclear con...

Primary effects 1.      Nuclear contamination 2.      Biological contamination 3.      Chemical contamination 4.      Destruction of infrastructure facilities. 5.

Respiration in fishes, RESPIR A TIO N IN FISHES - Respiratory org...

RESPIR A TIO N IN FISHES - Respiratory organs are gills. In elasmobranchi 5-7 pairs of gills present, spiracles present. In Teleostomai 4 pairs gills present, covered

Define human digestive system, Q. Which organs of the body is part of the h...

Q. Which organs of the body is part of the human digestive system? The digestive system also known as "systema digestorium" or gastrointestinal system is composed of the digest

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd