Define about the column chromatography, Biology

Assignment Help:

Define about the Column chromatography?

In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.

The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.


Related Discussions:- Define about the column chromatography

Biomass energy, Biomass energy Energy produced by burning biomass it is...

Biomass energy Energy produced by burning biomass it is known as biomass energy. When biomass is burnt, it releases stored chemical energy, which has accumulated in it through

Explain the dietary management in trauma, Explain the Dietary Management in...

Explain the Dietary Management in Trauma? Trauma As a result of metabolic responses to injury, there is an increase in the energy expenditure. Oxidation of body fat stores take

State the concept of type locus interaction, State the concept of type locu...

State the concept of type locus interaction Some of the major contributions included the concept of type locus interaction, the analysis of quantitative as opposed to qualitati

Test for venous function, Test for Venous Function Make patient s...

Test for Venous Function Make patient stand, apply a tourniquet at mid-thigh firmly enough to occlude the superficial veins, but not the deep veins or the arteries.

Measures to control malaria infection, Measures to control malaria infectio...

Measures to control malaria infection: Malaria is a communicable disease caused by the female anopheles mosquito. a) Controlling mosquito population : Mosquito population can b

Soil-environmental components, Soil-Environmental Components The word s...

Soil-Environmental Components The word soil is derived from Latin word 'solum' meaning earthy material in which plants grow. The soil is the consolidated outer layer of the ear

Vitamins, Vitamins Animals cannot sustain a healthy life if they are ...

Vitamins Animals cannot sustain a healthy life if they are fed on a diet having only carbohydrates, fats and proteins. They also require vitamins in small quantities in the r

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd