Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
Q. Describe Infection control sterilization protocol? Today is the age of modern technology coupled with medical challenges. Pervasive increase in serious transmissible disease
How much salt is in the human body? 50 Kilo human has about 7 tablespoons of salt inside there body.
When the standard free energy of a reaction is -5.7 kj/mol then at equilibrium a- there will be 10 times more products than reactants b-there will be 10000 times more product
A 37-year old woman is admitted to the hospital after complaining of chest pains. She admits to having had severe headaches for several days prior to seeking medical help. She is a
Explain Management of Ledge - Non-Surgical Endodontic Retreatment Place a bend of approximately 45 degree in the apical 1-2 mm of a #15 to 25 file. By gentle reciproca
C ampylobacter infection C a mpylobacter jejuni is the commonest species found in poultry. These are delicate organisms that may not survive outside the host unless prot
sea divisions
Q. How is the yolk sac formed? What is the function of the yolk sac? The yolk sac is formed from the covering of the vitellus by few cells originated from the primitive gut.
Omnispective Classification This is the extension of the concept of natural classification put forward by Black Welder (1967). The approach seems quite realistic and pragmatic.
How does aggregation occur in sickle-cell anemia? Place the steps in the correct order from no aggregation to sickle red blood cell. Note that deoxyhemoglobin is generally in the T
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd