Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
Consider the evolution of metabolic pathways. Some biologists believe that these pathways evolved from?
Define Health Benefits of Other Dietary Factors with Antinutritional Effects? Despite the predominantly nutritional antagonistic effects of the factors described above, there i
How last chamber of heart in cockroach receives blood without having ostia?
BEE DISEASES - Suffer from nosema diseases caused by Nosema apis. SILK WORM DISEASES - Pebrine disease caused by Nosema.
Q. Preparation of the Laboratory Samples From the primary sample while selecting a representative laboratory sample, we need to consider the following: a) Laboratory sampl
Explain about the Biofertilizers Biofertilizers, are the cultures of microorganisms used for inoculating seed or soil or both under ideal conditions to increase the availabilit
If experiments do not support the hypothesis, the hypothesis is always rejected or wrong. True or False?
Q. How is the transport of substances done across the bryophyte tissues? How is this feature related to the general size of these plants? In bryophytes there are no nutrient-co
Q. What are the three major types of passive transport? The three main types of passive transport are simple osmosis, diffusion and facilitated diffusion.
Dietary counseling based on the food pyramid concept and the food to target concept will be quite useful in the management of the disease. The optimal age for discontinuing the
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd