Define about the column chromatography, Biology

Assignment Help:

Define about the Column chromatography?

In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.

The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.


Related Discussions:- Define about the column chromatography

What are the parts of nervous system, What are the parts of Nervous system ...

What are the parts of Nervous system Nervous system is divided into three main parts: 1) The brain and spinal cord or Central Nervous System (CNS). 2) The nerves or Peri

Determine about the halstead''s method, Determine about the Halstead's meth...

Determine about the Halstead's method Reitan adopted Halstead's methods and various test procedures and with them established a laboratory at the University of Indiana. He supp

Microscope, How to calculate the magnifying power of a microscope i

How to calculate the magnifying power of a microscope i

What is oxidation and reduction, What is Oxidation and Reduction? Oxid...

What is Oxidation and Reduction? Oxidation and Reduction :   Most people are familiar with oxidation as it pertains to the rusting of metals. Oxygen grabs electrons from iron

Cavities in cork, Q. In 1665 Robert Hooke, an English scientist, published ...

Q. In 1665 Robert Hooke, an English scientist, published his book Micrographia, in which he explained that pieces of cork viewed under the microscope presented small cavities simil

Feeding on food masses, Normal 0 false false false EN-I...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Which sugars does not contains an o-glycosidic bond, Which of the following...

Which of the following sugars does NOT contains an O-glycosidic bond? Select one: a. amilose b. amylopectin c. glycogen d. cellulose

Palaeontological evidence of evolution, EVIDENCES OF EVOLUTION - The ev...

EVIDENCES OF EVOLUTION - The evidences of organic evolution derived from many lines of study indicate that present day forms have arisen by gradual changes from pre existing fo

Show examples of cnidarians, Q. What are few examples of cnidarians? In whi...

Q. What are few examples of cnidarians? In which environments can these animals be found? Hydra, Jellyfish, sea anemones and corals are good examples. All of them are aquatic,

Why the depicted food web is not complete, The depicted food web is not com...

The depicted food web is not complete, mainly because:  a. The number of producers is less than the number of consumers. b. There are no decomposers. c. Only secondary co

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd