Define about the column chromatography, Biology

Assignment Help:

Define about the Column chromatography?

In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.

The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.


Related Discussions:- Define about the column chromatography

What does secondary productivity in an ecosystem indicate, a) What does sec...

a) What does secondary productivity in an ecosystem indicate? b) List any two factors by which productivity is limited in aquatic ecosystems

Define intentional adulteration - types of adulteration, Define Intentional...

Define Intentional Adulteration - Types of Adulteration? In intentional adulteration, the substance is added, removed or substitute knowingly by the adulterator for the purpose

Describe the functionality of pectin, Functionality Pectins are mainly ...

Functionality Pectins are mainly used as gelling agents, but can also act as a  thickener, water binder and stabilizer. Low methoxyl pectins ( 2+ ions. Highly acetylated pecti

Cellular features of the beings of the plant kingdom, Q. What are the major...

Q. What are the major cellular features of the beings of the plant kingdom? The typical plant cells are eukaryotic (have nucleus), photosynthetic (use light to make food) and a

Classifications of carbohydrates based on number of aldehyde, Define Classi...

Define Classifications of carbohydrates based on number of aldehyde? Classification based on the presence of aldehyde or ketone group Carbon atoms Aldos

Define the parenteral nutrition support, Define the Parenteral Nutrition Su...

Define the Parenteral Nutrition Support? Parenteral nutrition support is the provision of dextrose, amino acids, electrolytes, vitamins, minerals and trace elements with or wit

Define the medical complications of bulimia nervosa, Define the Medical Com...

Define the Medical Complications of Bulimia Nervosa? The medical complications of bulimia nervosa range from fairly benign, transient symptoms, such as fatigue, bloating and co

Explain the spring clip model, Q. Explain the Spring clip model? The sp...

Q. Explain the Spring clip model? The spring clip model differs from the spring model in a way that the force applied is comparatively slower. This also depicts elasticity or p

What are the hormones secreted by the adrenal medulla, What are the hormone...

What are the hormones secreted by the adrenal medulla? What are their respective functions? The medullary portion of the adrenals secretes hormones of the catecholamine group:

Gene pool, Natural selection can therefore mean differential reproduction a...

Natural selection can therefore mean differential reproduction among members of the sarne gene pool. We earlier said that selection would promote those alleles that give rise to ph

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd