Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
The components of treatment of NKHDC are: 1) control of water loss. 2) control of sugar using insulin. 3) adjust electrolytes like sodium and potassium. 4) control infe
A large number of important drugs are derived from plants. For example Tubocuranin, derived from plant-based curare, is used as a muscle relaxant during surgery. Curianol, a Guy
D esig n of expression vectors Expressing a transgene in an appropriate manner is a huge task. The gene construct that is quite active in transfected cells may be only weakl
Material Basis of Primitive Life: In the primitive society, human beings invented tools for catching animals, and for collecting, transporting and even preparing food. They l
Shortness of Breath It is a subjective sensation of being unable too draw in enough air to breathe. Most often, this is associated with congestive cardiac failure and may
How plants use the pattern of changes in day/night length to solve the paradox that day length is equal twice a year. Plants possess the ability to balance the daily changes in
Q. What is the approximate pH of the salivary secretion? Is it an acid or basic fluid? What are the main functions of saliva? The saliva pH is approximately 6.8 it is therefore
What conclusion you can draw about microorganisms that you saw under microscope from the plaque of your teeth?
Q. What is the criterion used to classify hosts as intermediate hosts or as definitive hosts? The criterion used to categorize hosts as intermediate hosts or as definitive host
What type of larval dispersal does Dreissenids use?
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd