Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
LIPOIDA L MODELS According to Overton (1902) plasma membrane consists of single layer of lipid, because cell permeability is related to lipids. According to Gorter and
Q. What is the relation between the hypophysis and the thyroid? The hypophysis secretes TSH (thyroid- stimulating hormone). This hormone hastens the secretion of thyroid hormon
What are Sphingolipids? Also there link to cancer caused in cells.
Assume you were given a mixture consisting of one molecule each of all possible sequences of a smallish protein of molecular weight 4800 daltons. If the average molecular weight of
Anticoagulation in Prosthetic Valves The usual anticoagulants used are: (I) Warfarin sodium, and (2) Acitrorn (coumarin derivative). In patients who had thromboembolism
What are some functions of the epithelium? The epithelial tissues can do covering, impermeability and protection against the environment, for instance, in the skin, resorption
Mention the product and its use formed by each the microbes listed below: i) Lactobacillus ii) Streptococcus iii) Saccharomyces cerevisiae
Why Water and Electrolytes are important for exercise? Water and electrolytes are important for exercise performance as they maintain blood volume and osmolality, thermoregulat
Explain the relationship between an agonist muscle and its antagonist as it relates to positional control, functional movement and neuromuscular activation. For example, how the th
Complications of Exchange Transfusion i) Infection of umbilical vessels and systemic injection related to blood transfusion like malaria, CMV, AIDS and hepatitis B. ii) O
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd