Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
Define Sample Titration for Fehling Soxhlet Method Dilute the given sample glucose solution in 100 ml volumetric flask to the mark with distilled water. Pipette 5 ml of Fehling
Define the diseses Shigellosis Shigellosis or bacterial dysentery is caused by facultative anaerobic, gram-negative, non-spore forming, rod-shaped Shigella in the family enter
Q. What is the structure of the adult fern within which cells undergoing meiosis can be found? In these plants meiosis takes place within structures known as sorus (plural, sor
Indian tick typhus Indian tick typhus (Mediterranean spotted fever) is a tick-borne rickettsial infection caused by Rickettsia conori and is characterized by fever and a chara
A DNA library is a collection of cloned DNA fragments in a cloning vector which can be fined for a DNA of interest. If the target is to isolate particular gene sequences.
Define Regents for Estimation of Vitamin C - Colorimetric Method? 1. 2, 4 dinitrophenylhydrazine reagent - Dissolve 2 g of dinitrophenyl hydrazine in 100 ml of 9N sulphuric aci
Explain the Nutrition of Fungi? Fungi grow in moist environment and are chemoorganoheterotrophs. What do we mean by chemoorganoheterotrophs? The term chemotroph describes organ
Q. What are the venous of the valves system? What is their function? The valves of the venous system are structures inside the veins that permit blood to flow only in the norma
Define about the Ascorbic acid (vitamin C)? Ascorbic acid was discovered as the anti-scurvy vitamin. Vitamin C (chemical names ascorbic acid and ascorbate) is the six-carbon la
The excretion by the human kidneys 1. Descending limb of Loop of Henle is impermeable to water 2. Distal convoluted tubule is incapable of reabsorbing HCO3 3. Nearly 99 p
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd