Define about the column chromatography, Biology

Assignment Help:

Define about the Column chromatography?

In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.

The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.


Related Discussions:- Define about the column chromatography

What do you mean by nervous system, Q. What do you mean by Nervous System? ...

Q. What do you mean by Nervous System? Structural Organization:   CNS + PNS Functional Organization:   only concerned with PNS Sensory (Afferent) ->  CNS Motor (Effe

Agro industrial-sulphur, Sulphur Sulphur is a component of the animo a...

Sulphur Sulphur is a component of the animo acids methionine, cysteine and cystine; the B-vitamins, thiamin and biotin; as well as a number of the organic compounds. Sulphate,

How much vitamins should be taken for management of obesity, How much Vitam...

How much Vitamins should be taken for management of obesity? If adequate amount of fresh fruits and vegetables are included in the diet, the body stores of water soluble vitami

#title.protozoa classes., #question.give the classes of protozoa phylum in ...

#question.give the classes of protozoa phylum in detail with examples .

Diagnosis and management of cancer lung, Diagnosis Chest X-ray, Sp...

Diagnosis Chest X-ray, Sputum Cytology Test  Fibro Optic Bronchoscopy and Biopsy  In later stage of disease - weight loss, Debility indicating metastasis Staging of

Define advantages of blanching, Define Advantages of Blanching? Blanch...

Define Advantages of Blanching? Blanching as a pre-treatment before drying has the following advantages: 1. It helps in cleaning the material and reducing the amount

Concepts of epidemic disease and endemic disease, Q. What is the difference...

Q. What is the difference between the concepts of epidemic disease and endemic disease? The Endemic diseases are those that often affect people of a given place, many or few in

The rapeutic nurse, THE RAPEUTIC NURSE: Development of  therapeutic  n...

THE RAPEUTIC NURSE: Development of  therapeutic  nurse patient  relationship is the basic requirement  of nursing. A helping relationship  in nursing may be explained as an in

Explain complete and incomplete digestive system , Normal 0 fal...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Agro industrial-early embryonic mortality, Early embryonic mortality E...

Early embryonic mortality Embryo mortality is a serious problem for farmers, practitioners and researchers. The establishment and maintenance of pregnancy is a highly complica

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd