Define about the column chromatography, Biology

Assignment Help:

Define about the Column chromatography?

In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.

The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.


Related Discussions:- Define about the column chromatography

Explain the properties of gum karaya, Explain the Properties gum karaya ...

Explain the Properties gum karaya Gum karaya is water-swellable rather than water-soluble and absorbs water very rapidly to form viscous colloidal dispersions at low concentra

What do energy pyramids represent, Q. What do energy pyramids represent? ...

Q. What do energy pyramids represent? The Energy pyramids represent the amount of accessible energy in each trophic level of the food chain.

Phylum Echinodermata, define and explain Water vascular system with podia a...

define and explain Water vascular system with podia and tube-feet madreporite mostly present

Types of heart failure, The various types and their description of Heart fa...

The various types and their description of Heart failure are as follows:  Left Sided Versus Right Sided Heart Failure   Predominantly left sided failure is seen in left ventr

Respiration, explain the respiration that takes place in different organism...

explain the respiration that takes place in different organisms??

Causes of air pollution, Causes of Air Pollution We known about the c...

Causes of Air Pollution We known about the composition of atmosphere, its major and minor constituents. The composition of atmosphere has remained the same for thousands of y

Economic significance - conservation of wildlife, Economic Significance - C...

Economic Significance - Conservation of Wildlife Certain wild species are important because of their actual or potential economic value to people. Wildlife resources provide p

Prospects of meat products, P r o s p ec ts of meat products The ...

P r o s p ec ts of meat products The demand for convenience meat based fast food is ever increasing due to rapid industrialization and urbanization, higher standards of l

Illustrate about sterilization protocol, Q. Illustrate about Sterilization ...

Q. Illustrate about Sterilization protocol? Sterilization protocol includes proper transportation and cleaning of instruments followed by their packing. Items can be sterilized

Define the working of cyclomixer, Define the working of Cyclomixer? Thi...

Define the working of Cyclomixer? This is an electrical mixer used for mixing the contents of a test tube. The speed of the mixer can be adjusted to your requirement. This is a

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd