Define about the column chromatography, Biology

Assignment Help:

Define about the Column chromatography?

In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.

The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.


Related Discussions:- Define about the column chromatography

Modes of respiration, Modes of Respiration Many small organisms obtain...

Modes of Respiration Many small organisms obtain oxygen by diffusion through their body surfaces. They do not have any specialised respiratory organs nor do they have blood ci

What are some prophylactic measures for tapeworm infections, Q. What are so...

Q. What are some prophylactic measures for tapeworm infections? The major prophylactic measures against the taeniasis and the cysticercosis are not to ingest raw or badly cooke

Pesticides - danger to wildlife, Pesticides - Danger to Wildlife Anoth...

Pesticides - Danger to Wildlife Another recently realised danger to wildlife in many parts of the world has come from the development of more effective pesticides. As agricult

Main species of taenias that cause human diseases, Q. What are the two main...

Q. What are the two main species of taenias that cause human diseases? The two chief species of taenias that cause disease in humans are Taenia solium, or the pork tapeworm, an

Compare different communities for conservation purpose, "Diversity is good"...

"Diversity is good" is one of the axioms of conservation biology. However in absentia of historical information, why is noting higher diversity in a system NOT the best way to eval

Explain technologicai advances of clinical dietitian, Explain TechnologicaI...

Explain TechnologicaI advances of clinical dietitian TechnologicaI advances in nutritional  support for the critically ill have enhanced the clinical dietitian's role. In the

Define endocytosis of glut4 transporters, Define endocytosis of GLUT4 Trans...

Define endocytosis of GLUT4 Transporters Person X is a healthy human who has volunteered to take experimental drug Y.  Person X has a normal dinner at 6 PM on April 1 and then

Explain about the lactation process, Explain about the Lactation Process? ...

Explain about the Lactation Process? Lactation is a physiologic process which has profound relevance for both the mother and the newborn. It is the period following pregnancy w

Gymnosperm affinities, basic difference beyween gymnosperm,angiosperm and p...

basic difference beyween gymnosperm,angiosperm and pteridophytes.

Test cross, TEST CROSS Crossing of F hybrid with its homozygous recessi...

TEST CROSS Crossing of F hybrid with its homozygous recessive parent is called test cross and the progeny of test cross is called test cross progeny. A .      Monohybrid Te

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd