Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
Feeding Mechanisms of Animals All animals have evolved successful methods for extracting their required nutrition from the environment. Thus we find a diversity of feeding mec
Nitrogen Control of Nitrogen Assimilation N 2 -fixer like Klebsiella pneumoniae and Nostoc can grow with N 2 , NO - 3 or NH + 4 as nitrogen source. You would like to know
Explain Canal Obstruction - Non-surgical Endodontic Retreatment File work shorter than the normal apical and feel a great resistance to reach the true length a. A retain
What is the significance of proteins for living beings? Proteins play a fundamental role in nearly all biological processes. Because of their diversity they can assume many dif
What is the difference between an ecological niche and a habitat? An ecological place is a set of peculiar activities, resources and strategies that a species explores to repro
Explain the histologic techniques A clinician has to base the diagnosis only on the clinical and radiographic findings as microbiologic and histologic techniques are restricte
Q. What is a prion? The prion is an infectious (transmissible) protein able to replicate by transforming other proteins into a copy of the prion. The mechanism of the copying i
DEFICIENCY DISEASES Animals may suffer from diseases caused by deficiencies of energy and proteins, mineral nutrients and vitamins, which generally occur either due to deficiency
Describe the Technique of Operation in prosthetic valve endocarditis? The operative principle is drainage of abscess, removal of debris and valve rep or replacement to reverse
Anti Lipid Drugs : The current thinking is that it is very important to keep lipid levels low. It helps in preventing atherosclerotic changes in venous grafts and the long-term p
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd