Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
The ability to maintain myocardial perfusion at constant levels in the face of changing driving presence is termed autoregulation. In normal cases, autoregulation is maintained at
What is the function of vitamin E? In which foods can it be found? Vitamin E, or tocopherol, is a fat-soluble vitamin that participates as coenzyme in the respiratory chain, t
Weiss's Theory of Fibroosseous Fixation Weiss' theory stated that there is a fibro-osseous ligament formed between the implant and the bone and this ligament can be considered
Dr. Don McPhail and his colleagues hypothesized that the existence of the benthic and limnetic sticklebacks is the result of allopatric speciation. Although this may be true for so
Q. Determine ST-Segment Elevation? Ans. Exercise induced ST-segment elevation is common in subjects with post MI stress tests in leads where Q-waves are present. It has be
Colibacillosis Colibacillosis is caused by different strains of Escherichia coli. The disease mainly affects children and travelers. Organisms grow in gut and produce toxin. It ma
Respiratory Organs These are the organs regarded with the gaseous exchange, i.e., intake .of oxygen and output of carbon dioxide. They have generally greater rate of gas exch
PSYCHEDELI C DRUGS (HALLUCINOGENS) - They act mainly on CNS and greatly alter one's thoughts, feelings and perceptions, called psychedelic drugs. They cause optical or a
Explain Canning (temperature above 100° C) - method of food preservation? Canning is the process in which the foods are heated in hermetically sealed (airtight) jars or cans t
What is Metallic implants Metallic implants undergo one or more several surface modifications to enable them to become suitable for implantation. These modifications are passiv
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd