Define about the column chromatography, Biology

Assignment Help:

Define about the Column chromatography?

In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.

The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.


Related Discussions:- Define about the column chromatography

Thyroid, A person has swelling on the front of his neck. Name the disease h...

A person has swelling on the front of his neck. Name the disease he is suffering from and the cause of it.

Explain the chloride permeability, An impermeable membrane separates one li...

An impermeable membrane separates one liter of a 1M KCl solution in the left compartment from one liter of 1M NaCl solution in the right compartment.  At 2 AM today the membrane be

Illustrate wild life sanctuaries, Q. Illustrate Wild Life Sanctuaries? ...

Q. Illustrate Wild Life Sanctuaries? You must have seen how some of the parks are fenced. The intention is to keep the animals from wandering out and not to destroy neighbourin

Australopithecines, The first ever australopithecine fossil was found in 19...

The first ever australopithecine fossil was found in 1924 at Taung, South Africa. It was the skull of a 6 year old child showing a mixture of human and ape like features. This a

Explain composition of human milk, Explain Composition of Human Milk? R...

Explain Composition of Human Milk? Research clearly shows that each type of mammalian milk is unique and consists of a highly complex mixture of organic and inorganic compounds

Which mechanism is significant in death of erythrocytes, Of the following, ...

Of the following, which mechanisms are significant in the death of erythrocytes (pron: eh-rith-reh-sites) in human blood? Is it a) Phagocytosis (pron: fag-eh-seh-toe-sis) b)

What is the karyotype found in down syndrome, What is the karyotype found i...

What is the karyotype found in Down syndrome? Down syndrome is the aneuploidy that is a numeric alteration of chromosomes within the cells compared to the normal number of chro

What is class chondrichthyes, What is Class Chondrichthyes? Class Chond...

What is Class Chondrichthyes? Class Chondrichthyes takes its name from two Greek words. "Chondros"; means cartilage, and "ichthys"; refers to fish, therefore the name "cartilag

Diabetic foot are impaired blood supply to foot, Diabetic foot are impaired...

Diabetic foot are impaired blood supply to foot Diabetic foot are impaired blood supply to foot i.e. vasculopathy. This leads to ischaemia which in turn leads to delayed or non

Ventricular arrhythmias-peri operative problems, Ventricular Arrhythmias : ...

Ventricular Arrhythmias :  These include premature ventricular contractions (PVC), ventricular tachycardia (VT) and ventricular fibrillation (VT). At times they may be respon

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd