Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
Q. How do calcium ions participate in muscle contraction? Why do both muscle relaxation and muscle contraction spend energy? In the muscle cells calcium ions are stored within
How do calcium ions participate in muscle contraction? Why do both muscle contraction and muscle relaxation spend energy? In the muscle cells calcium ions are kept within the s
Habits of patients- Implants Smoking has been shown to predispose to poor bone quality by reduced vascularity. Therefore it is prudent not to accept heavy smokers as implant pa
Define about the Zinc Deficiency? Evidence suggests that nearly one-third of preschool children in lower-income countries have stunted growth, and that a considerable proportio
Briefly explain about the Sit-ups Test? To measure muscular endurance, bent knee sit-ups can be done. Sit-ups begin with the subject lying flat on their backs with their knees
POST-OPERATIVE CARE OF PATIENTS The first few days following cardiac operations are the most critical in terms of the patient's survival. The safety with which a patient can
Patients with heart failure have a high incidence of both symptomatic and asymptomatic arrhythmias. About 10 per cent of patients have syncope or presyncope resulting from ventr
A solid compound was found to contain 42.11% C, 51.46%O and 6.43% H by weight. The molecular weight of the compound was 341. What is the molecular formula of the compound?
Hypomagnesaemic tetany of calves Young calves in the age group of 2-8 weeks and fed whole milk or milk replacer are more likely to develop hypomagnesaemia due to inadequate ma
Monocotyledonous Embryo The early development of the proembryo in monocots follows the same pattern as in the dicots. However, at the time of differentiation in the globular
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd