Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
Regarding solubility, how are lipids classified? Fats and oils are hydrophobic molecules, i.e., they are non polar and insoluble in water. Lipids in general are molecules with
Explain the Characteristics of Agar- Agar? There are many characteristics, which make agar-agar, an ideal solidifying agent. These are: (a) It has high gel strength, so it c
when does it started?
Explain Chemolithoautotrophs - Nutritional Types of Microorganisms These microorganisms use inorganic compound as source of energy and electron and CO 2 as a source of carbon.
Competence of Larval Tissue or Multiple Responses The responsiveness of different larval tissues to thyroxine is markedly different. The tissues of the tail becomes necrotic
Define the Operations of a Public Nutritionist? A public nutritionist can perform the following: In the hospital-based set up, she is a part of the team delivering thera
Herb Stage - Xerarch The Soil-forming and soil-holding reactions of the mosses are so pronounced that the seeds of various xerophytic herbs, especially short-lived annuals: ar
Define Chromatography - Basic Separation Technique? Chromatography is the most effective technique for the separation, purification and identification of one or more biological
Role of exercise in the management of diabetes mellitus Exercise is another important component of life style modification. Like diet control, regular exercise is important in
Define the term Hot water blanching? A appropriate water-blanching process in traditional processing is as follows: The sliced material is placed on a square piece of cl
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd