Define about the column chromatography, Biology

Assignment Help:

Define about the Column chromatography?

In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.

The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.


Related Discussions:- Define about the column chromatography

Infectious coryza, I nfectious coryza A highly infectious bacterial di...

I nfectious coryza A highly infectious bacterial disease of chickens caused by Hemop hilus paragallinarum, is characterized by catarrhal inflammation of the upper respirator

Classification of multicellular animals, Classification of Multicellular An...

Classification of Multicellular Animals The multicellular animals (Animalia) originated from the simpler protozoan protists. You know the simpler multicellular animals further

Describe metabolism, Q What is metabolism? Metabolism is the set of che...

Q What is metabolism? Metabolism is the set of chemical and physical processes upon which the life of the cells of a living being depends.

Vigorous or vigorously active lifestyles - physical activity, Define Vigoro...

Define Vigorous or vigorously active lifestyles - physical activity? These people engage regularly in strenuous work or in strenuous leisure activities for several hours. Examp

What is the cost-benefit relationship, What is the cost-benefit relationshi...

What is the cost-benefit relationship regarding sewage treatment as a method to fight water pollution? To treat sewage is much cheaper for society. The non treated sewage pollu

Db is a standard abbreviation, DB is a standard abbreviation used for the q...

DB is a standard abbreviation used for the quantitative expression of: 1. the density of bacteria in a medium 2. a particular pollutant 3. the dominant Bacillus in a cultu

Show appearance of food product, Q. Show Appearance of food product? Ap...

Q. Show Appearance of food product? Appearance can be judged by the eye. Appearance factors include such things as size, colour, uniformity, absence of any defect, shape, whole

Explain about oil-bearing fruits, Explain about Oil-bearing fruits Oil-...

Explain about Oil-bearing fruits Oil-bearing fruits, nuts and seeds have been grown and used for food for many centuries. More than 100 varieties of plants are known to have oi

Gonad glands, what are the gonad gland disorders and what do they do

what are the gonad gland disorders and what do they do

State the amount of micronutrients in fertilizers, State the amount of micr...

State the amount of micronutrients in fertilizers The amount of micronutrients in fertilizers must be much more carefully controlled than the macronutrients. The difference be

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd