Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
Explain Food Applications of gum karaya The water absorbing and water-holding capacity of Karaya, together with an excellent acid compatibility made it suitable for its use
Workgroup A Workgroup is a group of people who are working together on the same project. The members of such a group are usually (not always) located in close physical proximi
Ultrasonography: Ultrasounds are sound waves with a frequency of higher than the upper range of human hearing i.e., approximately 20,000 cycles per second (20 kilohertz).
Explain the Chemical Methods for Control of Microorganisms? Different chemicals can be used which can act as disinfectant, antiseptics or even chemical sterilants. These are:
what is the respiration interdisiplinary field,legal concerns,backgroun and ad vantages and diadvantages
Minerals :- Magnesium Food Source Whole grains, nuts, legumes, green leafy vegetables Nutritional Functional role Essential nutrient: Deficiency is rare Co
Q. Categories of value of biodiversity? The value of biodiversity is often divided into two main categories: · UTILITARIAN (also known as instrumental, extrinsic, or use) v
Q. Energy during myocardial infarction? As mentioned above, patient who have recently suffered from an attack of myocardial infarction are hospitalized in the intensive cardiac
Tularaemia The disease is known by different names like deer fly fever, rabbit fever, Ohara’s disease or tularensis. It is an acute, febrile, granulomatous infection, caused b
The F2 phenotypic ratio from a dihybrid cross with epistasis was 9:3:4. If instead of a dihybrid cross, a double heterozygote was test crossed (crossed to a homozygous recessive in
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd