Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
Determine the term -Stolon. In a number of colonial cnidarians, ascidians, and lophophorans, a cord of tissue connects different zooids of colony to each other. Buds on stolon
Q. Explain the Bone Implant Interface? Three phases have been described in the development of bone implant interface. 1. Stabilization phase Subendosteal and subperiost
DRUG DEPENDENCE - Some drugs are prescribed by doctor for the prevention or treatment of a disease. Some drugs are used for long periods. A person becomes addict to these
Q. What is the chorioallantois membrane present in the embryonic development of birds and reptiles? How does this membrane participate in the energetic metabolism of the embryo?
Define cause of vitamin a deficiency - Infections? During acute infections, vitamin A intake in preschool children is reduced due to impaired appetite and impaired vitamin A ab
Define Free Solution or Moving Boundary Method? The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius
This species is an ornamental tree in China, and has yielded the clinically active agents topotecan, irinotecan, and 9 -aminocamptothecin, which are semisynthetically derived from
Carpentier -Edwards Valve (C-E) : This is porcine aortic valve preserved in gluteraldehyde and mounted on flexible stent made of cobalt-chromium-nickel alloy (ELGILOY). Glut
(i) Genetic Bio- Diversity: All forms of life on earth contain genes. Genes are carrier of hereditary characteristic from one generation to another. " genetic diver
note
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd