Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
what animals use indirect respiration?
Selenium and vitamin E deficiency Selenium is an essential micro-mineral required for various physiological functions and for immunity against a variety of diseases. It is an i
What is Osmosis ? Osmosis : Osmosis refers to the diffusion of water across a semi-permeable membrane from an area of higher concentration of water to an area of lower con
Analysis of the written reports of service in relation to the fitness, quality and efficiency of the service should be provided. The routine record-checking is always insuffic
Q. Analyze the Regional Wall Motions? Ans. Various attempts have been made in the past to try and quantify the ischemic myocardium, however till date the best and most wid
Response to Wounding Wounding of tomato plants by mechanical injury or chewing by insects releases a factor that triggers accumulation of two proteinase inhibitors-throughout
State about the Halsted Reitan battery The Halsted Reitan battery, as the procedure came to be known over the years, also has a history. It has been described as a fixed batter
Adaptation Closely related to irritability is the capacity of living things for adaptation. You might have noticed that whenever you go outside in the bright sun after having
advantages and disadvantages of protozoa
Platyhelminthes (Flatworms) - Regeneration in Invertebrates Between flatworms the turbellarians, (mostly fresh water species and fresh water and terrestrial triclads) routinel
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd