Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
Zinc deficiency Zinc is essential for several physiological functions in the body such as protein synthesis, carbohydrate and nucleic acid metabolism, and foetal growth. The de
Q. Classify biomaterials and list some examples? Biologic classification is based on tissue response and systemic toxicity effects of the implant and is divided into three clas
What are the Biotolerant materials Biotolerant materials, are characterized by a thin fibrous tissue interface. The fibrous tissue layer develops as a result of the chemical pr
Explain the Basal Metabolic Rate? The Basal Metabolic Rate (BMR) gradually decreases after the attainment of maturity due to a decrease in muscle mass tissues and physical acti
PITS Depressions in secondary cell wall is called pit. A pit present on the free cell wall surface without its partner is called Blind pit. It consists of 2 parts -
Define Reagents Required and Methodology for Mucic Acid Test? - Sugar solution - Conc. Nitric acid Methodology Take about 50 mg of sugar in a test tube. Add 1 ml
ELIZA stands for enzyme linked immune diffusion assays. It works on standard of antigen antibody reaction.
Q. What is the major cell organelle involved in cell digestion and what are the properties of that organelle that enable it to do the task? The organelles accountable for intra
What is Working of Mouth in human body? Food enters the digestive system through the mouth, where it is processed by the teeth and mixed with saliva, prior to being swallowed.
Define Fluids requirement to avoid underweight problem? Take fluids only after a meal instead of with or before meals so that food intake is not reduced. High calorie nourishin
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd