Define about the column chromatography, Biology

Assignment Help:

Define about the Column chromatography?

In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.

The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.


Related Discussions:- Define about the column chromatography

Spleen, SPLEEN Largest lymph gland, also with myeloid tissue is an i...

SPLEEN Largest lymph gland, also with myeloid tissue is an important specialized reticuloendothelial organ in vertebrates, as the site of erythropoiesis. Splenic tissue i

Uricotelism, Normal 0 false false false EN-IN X-NONE ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Explain H - shaped incision, Q. Explain H - Shaped Incision? This type ...

Q. Explain H - Shaped Incision? This type of incision is especially useful for: The anterior maxilla or mandible. Accurately identified implant position. Exposure o

Zoology, Classification of the Phylum Protozoa up to orders

Classification of the Phylum Protozoa up to orders

Define role of leptin in controlling gene expression, Define role of Leptin...

Define role of Leptin in controlling gene expression? Leptin is a protein product (containing 167 amino acids) of the ob gene expressed exclusively in the adipose tissue. This

Sensitive/critical periods: windows of enhanced plasticity, There are speci...

There are specific sensitive/critical periods in development during which acquisition of certain skills is substantially easier than at other times. In the animal domain there are

Define about the term indicators, Define about the term Indicators? The...

Define about the term Indicators? The easiest way to determine when the reaction is complete is by using indicators. An indicator is a compound that has a physical property (us

Explain about the dysphagia, Explain about the Dysphagia? Dysphagia is ...

Explain about the Dysphagia? Dysphagia is the inability to swallow or difficulty in swallowing. It is a common problem in those with neurological disorders and can occur in any

What is barfoed test and explain its principle, What is Barfoed's test and ...

What is Barfoed's test and its principle? This test is a specific test for monosaccharides. Principle This test is also a copper reduction test but differs from Fehling

Explain cap site, Cap site Two usages are given below: 1] In eukaryotes...

Cap site Two usages are given below: 1] In eukaryotes, the cap site is position in the gene at which transcription begins, and really should be called the "transcription initia

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd