Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
Normal 0 false false false EN-IN X-NONE X-NONE
Explain Back pain, arthritis and gout - Effect of Obesity? Abdominal obesity increases the risk of back pain because of the extra load on the spinal column. This, in turn, red
Aortic Valve replacement : The initial steps of the operation have been described earlier. Ascending aorta is cannulated. A single two-stage atrio-venous cannula is inse
Q. Show Gastrointestinal Diseases and Disorders? Before discussing the many gastrointestinal problems, it is useful to understand the basic physiology of the gastrointestinal t
Q. Explain threaded implants? Cylindrical non-threaded implants poorly distribute compressive forces and generate shears forces that may fragment and break the bone surrounding
Explain the Community ecology of disease? Ebola, hantavirus, bubonic plague, Lyme disease, West Nile virus, rinderpest, and canine distemper virus are all instances of diseases
What are chylomicrons? Yes, so you now know that chylomicrons are basically lipoprotein molecules which are water miscible. They are poured into lymphatic vessels through lacte
Enrichment of soil Indirect provision of minerals to grazing livestock includes mineral fertilization of pasture and altering soil pH, however this may not be always feasible
diff b/w protonephridia and metanephridia
Alterations occurring in fish The skeletal muscle of fish consists of short fibers arranged between sheets of connective tissue. The connective tissue in the fish muscle is les
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd