Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
Goat and sheep M. mycoides subsp. capri is the cause of caprine pleuropneumonia, a serious malady of goats. The disease is prevalent in certain parts of this country. The mort
Q. Supportive Therapy for diabetes patient? Certain foods, part of food or food components have been found to be beneficial in managing hyperglycemia. Most of these have been i
Define the Utilization of Most Probable Number Techniques? The MPN technique (multiple tube technique) can be used to determine the presumptive coliform count in the food. The
Define Procedure for estimation of vitamin c - colorimetric method? Carry out the experiment following the instructions given herewith: 1. Prepare a series of tubes for blan
Are the phloem and the xylem made of living cells? The cells of phloem are living cells and the cells that constitute the xylem ducts are dead cells killed by the lignin deposi
The secondary protein structure is produced by the manner its amino acids interact by intermolecular bond. These interactions make a spatial conformation of the polypeptide filamen
Q. What are the main factors that affect the growth of a population? The major factors that make populations grow are births and immigration. The major factors that make popula
Q. What do you mean by Alpha diversity? Alpha (a) richness refers to the number of species found in small, homogenous area. Alpha diversity refers to the diversity in a partic
Q. Minerals requirements for dyslipidemia? Minerals: The three most important minerals are chromium, zinc and magnesium. These minerals play a critical role in maintaining p
How is the respiratory system of beings of the phylum Annelida characterized? Respiration in annelids can be cutaneous or branchial. Cutaneous respiration happens due to the r
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd