Define about the column chromatography, Biology

Assignment Help:

Define about the Column chromatography?

In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.

The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.


Related Discussions:- Define about the column chromatography

Insulin dependent diabetes mellitus, Q. What is Insulin Dependent Diabetes ...

Q. What is Insulin Dependent Diabetes Mellitus? Mostly children and adolescents suffer from this type of diabetes however; it may appear in adults and elderly too. In this, the

26 The Human Impact on the Environment, What are the principal sources of e...

What are the principal sources of excessive nitrate and phosphate in rivers and lakes?

Define absorption of calcium from dietary calcium supplement, Define Absorp...

Define Absorption of Calcium from dietary calcium supplements? Absorption from dietary calcium supplements is important to know, since they are almost universally recommended f

List the functions of insulin, Q. List the functions of insulin? a) Ins...

Q. List the functions of insulin? a) Insulin controls the level of the sugar (glucose) in the blood. b) Insulin permits cells to use sugar (glucose) for energy. Cells cannot

Define thrombospondin polymorphisms, Q. Define Thrombospondin Polymorphisms...

Q. Define Thrombospondin Polymorphisms Thrombospondin polymorphisms may present an initial insight into our understanding of the genetic contribution to coronary atherosclerosi

Explain the micronutrients requirements during sepsis, Explain the Micronut...

Explain the Micronutrients Requirements during Sepsis Micronutrients: The requirement of almost all vitamins and certain mineral increases due to infection and inflammation. In

gas exchange, what are the organs of respiration in the lower form of anim...

what are the organs of respiration in the lower form of animals?

Nutrient absorption, Nutrient Absorption Except for carbon, oxygen and...

Nutrient Absorption Except for carbon, oxygen and hydrogen which are provided by CO 2 and water, all the other elements essential for plants are provided by the soil. Hence,

What is short bowel syndrome, Q. What is Short Bowel Syndrome? Short bo...

Q. What is Short Bowel Syndrome? Short bowel syndrome is a group of problems affecting people who have /had half or more of their small intestine removed. The massive resection

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd