Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
what is the difination of osmosis
Q. Working of serotonergic cell bodies? The major site of serotonergic cell bodies is in upper pons and the midbrain, specifically the median and dorsal raphe nuclei and to a l
Nutritional and functional role of minerals in foods Essential elements including the main elements and a number of trace elements fulfill various functions: as electrolytes, a
Does every gene mutation cause alteration in the protein the gene normally codifies? Not each gene mutation causes alteration in the composition of the protein the gene codifie
How dynamic biological processes represented as network? Many dynamic biological processes throughout a wide range of ecological scales are easily represented as dynamical netw
Q. What is Sulphonylureas? Sulphonylureas: Tolbutarnide is the mildest and its effect remains for 8 hours hence 2-3 doses need to be taken. Another drug chlorpropamide needs on
General characteristics of phylum
What is the life cycle of the hookworms? Adult hookworms within the human intestine release eggs that are eliminated with the human feces. Under adequate conditions of moisture
Endocytosis and Exocytosis Endocytosis is the process by which substances are brought into the cell from outside by formation of membrane bound vesicles. These extra cellular sub
Functionality of implant material It should take maximum advantage of available bone and permit the maximum amount of forces to be transmitted through the implant within physio
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd