Define about the column chromatography, Biology

Assignment Help:

Define about the Column chromatography?

In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.

The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.


Related Discussions:- Define about the column chromatography

Operculum - seed appendages, Operculum - Seed Appendages The term oper...

Operculum - Seed Appendages The term operculum is applied to a plug-like structure formed in the micropylar portion of the seed by proliferation of cells at the tip of the inn

Nuclear transfer method for gene transfer, Nuclear transfer method: Nucle...

Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human

Intravenous route, Intravenous Route This route of administering drug...

Intravenous Route This route of administering drugs is becoming more popular in the paediatric practice. Purpose of this Route When rapid action is required.

Energy requirement during congestive cardiac failure, Q. Energy requirement...

Q. Energy requirement during congestive cardiac failure? Energy: Composition of the calorie requirements on the basis of body weight is usually not feasible due to the presence

Explain hazard, Explain Hazard Hazard  :  A  biological, chemical  or...

Explain Hazard Hazard  :  A  biological, chemical  or  physical agent that is reasonably likely  to cause illness or  injury in the absence  of  its control.

Photosynthesis rate varies according to the photic energy, Photosynthesis r...

Photosynthesis rate varies according to the photic energy intensity. Do the similar occur in aerobic respiration? What happens to the glucose balance as a result of these variation

What are the genotypes, What are the genotypes and respective blood types o...

What are the genotypes and respective blood types of the ABO system? Since the alleles are IA, IB and i the possible genotypes are IAIA (blood type A), IAIB (blood type AB), IB

Removal of a patient''s peripheral intravenous catheter, Removal of a patie...

Removal of a patient's peripheral intravenous catheter resulted in brief bleeding and the loss of a small amount of blood. Which of the following processes occurred during the form

Zoonoses disease-salmonellosis, Salmonellosis Salmonellosis is an infe...

Salmonellosis Salmonellosis is an infection caused by bacterial species belonging to the genus Salmonella and usually associated with the ingestion of food containing salmonel

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd