Define about the column chromatography, Biology

Assignment Help:

Define about the Column chromatography?

In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.

The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.


Related Discussions:- Define about the column chromatography

Prevention of coronary heart diseases, Q. Prevention of Coronary Heart Dise...

Q. Prevention of Coronary Heart Diseases? In view of the steep rise in the incidence of chronic degenerative coronary heart diseases, several programmes have been formulated to

Undescended testes (cryptorchidism), Undescended Testes (Cryptorchidism) ...

Undescended Testes (Cryptorchidism) Undescended testes is a ccmmon disorder. It may be unilateral and may be classified as ectopic cryptorchidism, when the testes are normal

Regulation of ammonia assimilation, Regulation of Ammonia Assimilation ...

Regulation of Ammonia Assimilation Heterotrophic bacteria like Escherichia coli and Klebsiella aerogenes induce the operation of GS-glutamate synthase pathway of ammonia assim

Formula concentration and supplementation - calorie density, Define Formula...

Define Formula Concentration and Supplementation - Calorie Density? Formula Concentration can be done by decreasing the amount or water added in the mixing of the formula. Sta

How does chewing food help to speed up digestion, How does chewing food hel...

How does chewing food help to speed up digestion? Chewing reduces food to portions small sufficient to be swallowed and enhances the surface area of the food for digestive enzy

Explain starch, Starch It is a plant polysaccharide synthesized by  the...

Starch It is a plant polysaccharide synthesized by  the plant by photosynthesis and stored mainly  in  grains,  legumes,  roots  and  tubers.  Its molecular formula is (C 6 H 1

Describe about the term - neuropsychological assessment, Describe about the...

Describe about the term - Neuropsychological assessment Neuropsychological assessment has its roots in neurology, the branch of medicine that focuses on the nervous system and

Mass extinction, Mas s Extinction - Since beginning many species ev...

Mas s Extinction - Since beginning many species evolved and got extinct (in fact only 1 % is living). Sometimes it occured suddenly at large scale, (most recently dinosa

Explain performance testing of fats and oil, Explain performance testing of...

Explain performance testing of fats and oil In these cases, performance testing is the only means for evaluating the ability of fat or oil to perform the desired functions in a

Explain biochemical mediators of calcium metabolism, Explain biochemical me...

Explain biochemical mediators of calcium metabolism Bone physiology is controlled by an interaction of mechanical and metabolic factors. Under physiologic circumstances, bone

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd