Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
Q. What is Atherosclerosis? Atherosclerosis is an arterial lesion characterized by patchy thickening of the intima (innermost coal of artery) comprising of fat and layers of co
Embryonic stem cells: can differentiate into all cell types of the specialized embryonic tissues when given sufficient and necessary stimulation for a specific cell type, e.g. Plu
Define Requirements of Fat in Postoperative Nutritional Care Adequate amount of fat is needed to build up and maintain tissue fat reserves. Depending upon the existing health a
Nursing Management Propped up position. Monitor signs and symptoms and laboratory test results documenting myocarditis. Monitor adequacy of cardiac output and hemo
what cell organelle does photosynthesis take place in?
Define symptoms in Nervous system of pernicious anaemia? Parasthesia (numbness and tingling) occurs in fingers and toes. Occasionally, there are objective signs of involvement
Lipids Lipids are oily or greasy organic compound forming about 3% of protoplasm. These are also compound of carbon hydrogen and oxygen but unlike carbohydrates. The hydrogen a
What are nutritional Care related to Neurological Disorder? Let us then move on to the nutritional care. The important goals of nutritional care are to: prevent further
What is the difference between an ecological niche and a habitat? An ecological place is a set of peculiar activities, resources and strategies that a species explores to repro
Four Distinct Phases of Demographic Transition Demographic transition takes place in four distinct phases. 1. Re-industrial stage in which harsh living conditions lead to
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd