Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
In continuous wave Doppler, the transducer has two Doppler crystals, one to constantly transmit and one to continuously receive. As a result of this continuous mode of tra
Congenital Syphilis A positive serological test for syphilis in a newborn without stigmata of syphilis may be due either to passive transfer of maternal antibodies or to prena
Canine parvovirus infections The canine parvovirus infections are caused by a virus, which belongs to the genus Parvovirus in the family Parvoviridae. This virus is similar to
Define Endocrine or Hormonal Disorders - Infertility Infertility can be caused by endocrine disorders, long-term sequelae of sexually, transmitted infections, puerperal sepsis,
What is the role of platelet plug formation in hemostasis? What events occur during platelet plug formation? Describe the circumstances that may induce inappropriate platelet plug
Practical Points in Preparation of Nursing Care Plan You first review the nursing diagnosis, then assign this priority or the order in which these are to be met. Alo
Hello I have been working with reptiles for a while and have been researching the incubation method known as egg suspended. This method is used with birds. Its placeing an egg on a
Equine viral arteritis Equine viral arteritis (EVA) is a highly contagious disease of equines and is caused by equine arteritis virus (EAV), a RNA virus belonging to the genus
How to evaluate blanching efficiency? Finally, let us learn how to evaluate blanching efficiency? Normally, two of the more heat resistant plant enzymes, namely peroxidase and
What is the type of digestion that occurs in cnidarians? These animals have a digestive cavity and they make intracellular and extracellular digestion. The extracellular digest
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd