Define about the column chromatography, Biology

Assignment Help:

Define about the Column chromatography?

In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.

The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.


Related Discussions:- Define about the column chromatography

Define end and the beginning of the first interphase period, Q. What are th...

Q. What are the events that mark the end and the beginning of the first interphase period? What happens inside the cell in this period? The initial interphase period is the G1.

Define the effect of scfa on sodium absorption, Define the effect of SCFA o...

Define the effect of SCFA on Sodium Absorption? SCFAs (short chain fatty acids) have a stimulatory effect on sodium absorption from colonic lumen. The unionized SCFA crosses th

Downers cow syndrome, D o w n e r s cow syndrome The Downer's co...

D o w n e r s cow syndrome The Downer's cow, otherwise known as creepers, is commonly a fatal condition of dairy animal associated with the unsuccessful treatment of cas

Show the error detection and editing function, Q. Show the Error Detection ...

Q. Show the Error Detection and Editing Function? Error Detection and Editing Function: Consciousness helps us avoid acting solely through habit. It allows us to make novel res

Treatment and management of hypertension, Q. Treatment and Management of Hy...

Q. Treatment and Management of Hypertension? The first choice of treatment and management of primary hypertension is through behaviour modifications pertaining to food choices

Biodiversity as products, Individual components of biodiversity provide an ...

Individual components of biodiversity provide an unbeliev able wide range of products that are used to enhance the lives of people in all countries of the world. It is the diversit

What are the three main types of rna, What are the three main types of RNA?...

What are the three main types of RNA? What is meant by heterogeneous RNA? Messenger RNA, or mRNA, transfer RNA, or tRNA, and ribosomal RNA, or rRNA, are the three main kinds of

What is phospholipids, What is Phospholipids ? Phospholipids :  Ph...

What is Phospholipids ? Phospholipids :  Phospholipids are similar to triglycerides, except that they contain only two fatty acid, and one or both of the fatty acids have

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd