Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
Which one of the following is an example of ex-situ conservation? 1. Wildlife sanctuary 2. Seed bank 3. Sacred groves 4. National park Seed bank
hi there,i would like u to help me with understanding Hutchinson phyletic dieta
Explain the Classification of simple proteins? 1) Albumins: Proteins such as egg albumin and serum albumin are soluble in water and coagulable by heat. 2) Globulins: These p
Q. What is the function of the secretions of the seminal vesicle, prostate and bulbourethral glands in reproduction? These secretions along with sperm cells from the testicles
Use of Alternate Metabolic Pathways Plants surviving under water-logged conditions offer the simplest example of such a response. The submerged parts of such plants use the an
What is Vacuole ? Another structure found only in plant cells is the large central vacuole. The vacuole stores enzymes and waste products, in addition to providing the turgor p
PROVIDE a specific example for epithelial and connective tissues, how the arrangement of cells helps with tissue functioning?
How to calculate the raw score for each scale The raw score for each scale is the sum of the 0, 1 and 2 item scores. Thus, the higher the score, the poorer the performance. Th
Before the emergence of life of what gases was the earth's primitive atmosphere constituted? The earth's primitive atmosphere was basically produced of methane, hydrogen, ammon
Explain the Etiology and Clinical Features of Parkinson's disease? The cause of Parkinson's is unknown. Genetic predisposition (in most cases the reason for the death of these
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd