Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
The eukaryotic cell The eukaryotic cell is a complex unit and contains many structural features not seen in in the simple prokaryotic cell. Fig below shows the details of vario
It corresponds to the anterior and inferior interventricular grooves. Its right side is convex and bulges to the right ventricle. The septum has a posterior border which separates
Why doesn't a long lasting vaccine against common cold exist yet? Viruses that show a high mutation rate like the virus that causes the common cold escape simply from the actio
LOCOMOTION IN LEECH - Leech moves by looping action of its body on substratum. Posterior sucker is attached to base, body elongates, then anterior sucker is attached. Now po
Q. Signs and Symptoms diabetic ketoacidosis? The following are the signs and symptoms of DKA: 1) Increased heart rate (tachycardia) 2) Low blood pressure 3) Dehydratio
Define About the Sterols - Non Glyceride Fractions? These constitule a major proportion of the non-glyceride component while tocopherols, carotene pigments and flavour compound
Question 1: (a) Describe the maturity stages in fish. (b) "k" and "r" selection strategies are commonly used to describe the response of fish to biotic and abiotic cond
Q. What do you mean by Pericardium? Pericardium is the sac covering the heart. Pericardium consists of two layers-the visceral pericardium (epicardium) and the parietal pericar
Burrowing - Mechanics of Locomotion Some polychaetes are burrowing. Instance is glycerides and capitellidae. Their parapodia are smaller. Burrowing is done by protrusion of pr
Genetic engineering has been successfully used for producing: 1. Transgenic mice for testing safety of polio vaccine before use in humans 2. transgenic models for studying ne
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd