Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
what is the taxonomic classification for the protist Archaeplastida?
Q. Are all pneumonias caused by bacteria? The Pneumonia is the generic name of inflammation of the lungs. Moreover bacterial pneumonias, there are pneumonias caused by fungi, v
Adsorption - Colloidal particle Colloidal particles attract and hold to their surfaces the molecules of various gases, vapors and other matter with which they come into contact
Q. How is carbon dioxide released by cellular respiration transported from the tissues to be eliminated through the lungs? In vertebrates almost 70% of the carbon dioxide is ca
Explain about Simple proteins Simple proteins are those which are made of amino acid units only joined by peptide bond. Upon hydrolysis they yield a mixture of amino acids and
Describe about Psycho-social Change in children? Adolescence is a period of maturation for both mind and body. Along with the physic4 growth, emotional and intellectual develop
Natural selection can therefore mean differential reproduction among members of the sarne gene pool. We earlier said that selection would promote those alleles that give rise to ph
Make a detailed study on Phylum Protozoa taking an example with clear labeled diagrams.
Define the role of riboflavin in Drug and lipid metabolism? Flavoproteins catalyze dehydrogenation reactions, as well as, hydroxylations, oxidative decarobxylations, deoxygenat
Secondary Succession - Community Change Secondary succession is the sequential development of biotic communities after the complete or partial destruction of the existing comm
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd