Define about the column chromatography, Biology

Assignment Help:

Define about the Column chromatography?

In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.

The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.


Related Discussions:- Define about the column chromatography

Explain the sticky films - food microbiology, Explain the Sticky Films - Fo...

Explain the Sticky Films - Food Microbiology? Sticky films or tape are pressed against the surface to be assessed. Exposed film/tape is then pressed on the agar plate and analy

Special situations of pulmonary embolism, Q. Special situations of Pulmonar...

Q. Special situations of Pulmonary Embolism? The CXR is often abnormal in pulmonary embolism. Atelectasis and other focal pulmonary parenchymal abnormalities are the most commo

Cyanide - enzyme-activity control, Cyanide - Enzyme-activity Control P...

Cyanide - Enzyme-activity Control Plants generate cyanide from cyanogenic glucides and histidines. Ethylene biosynthesis is also accompanied by cyanide production. Nitrate red

Dietary management of gastritis, Q. Dietary management of gastritis? Pr...

Q. Dietary management of gastritis? Prompt medical care is successful in the management of an acute attack of gastritis only if it is accompanied by efficient and judicious nut

Does sunlight kill bacteria, Does sunlight kill bacteria? Inoculate two...

Does sunlight kill bacteria? Inoculate two sterile bacteria dishes from a dish where bacteria are growing. Place single dish in the open sunlight and the other in a warm dark p

Basic working of midbrain, Q. Basic working of Midbrain? Midbrain: Also...

Q. Basic working of Midbrain? Midbrain: Also known as the mesencephalon, this part is located behind the frontal lobes and in the center of the entire brain. It deals with func

Agro industrial-baling and cubing, Agro Industrial-Baling and cubing Ba...

Agro Industrial-Baling and cubing Bailing is one of the most common processes used for the conservation of green fodders and grasses in many developed countries and attempts ha

Define affluence availability of food as factor for obesity, Define Affluen...

Define Affluence and abundant availability of food as factor for obesity? With increasing affluence, increase in purchasing power and abundance of food, people tend to eat more

Blood be enhanced during vigorous activity, The percentage of oxygen absorb...

The percentage of oxygen absorbed from the air in the lungs is always about the similar, so how can the oxygen supply to the blood be enhanced during vigorous activity? Breathi

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd