Define about the column chromatography, Biology

Assignment Help:

Define about the Column chromatography?

In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.

The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.


Related Discussions:- Define about the column chromatography

Define ecosystems science and large-scale ecology, Define Ecosystems scienc...

Define Ecosystems science and large-scale ecology? Many of today's most pressing problems are regional or global in nature including for instance, the effects of land cover and

Dietary management during atherosclerosis, Q. Dietary management during ath...

Q. Dietary management during atherosclerosis? Dietary management and the nutrient requirements during atherosclerosis remain the same as for the management of dyslipidemia. Hen

Difference between an ecological niche and a habitat, What is the differenc...

What is the difference between an ecological niche and a habitat? An ecological niche is a set of peculiar activities, resources and methods that a species explores to survive

Transplantation, Transplantation - It is implanting of a living or p...

Transplantation - It is implanting of a living or preserved tissue or organ from one site to another side in the same individual or from one individual called donor to recip

What is the structural depiction of a carboxyl group, Q. What is the struct...

Q. What is the structural depiction of a carboxyl group? Carboxyl groups have a carbon attached to one hydroxyl group by a simple bond and one oxygen by a double bond. The othe

Define the features of phylum nematoda, Define the Features of Phylum Nemat...

Define the Features of Phylum Nematoda? Phylum characteristics of Nematoda which are the world's most abundant multicellular organisms are as follows: 1. Nematodes occur in

Explain the effect of deficiency of thiamin, Explain the Effect of Deficien...

Explain the Effect of Deficiency of Thiamin? Thiamin deficiency causes the disease beriberi in human beings, which has been classically considered to exist in dry (paralytic) a

Explain about vitamin C, Vitamin C (Ascorbic acid) Vitamin C, ascorbic ...

Vitamin C (Ascorbic acid) Vitamin C, ascorbic acid is also called anti-scorbutic vitamin. Ascorbic acid occurs as white crystals or white crystalline powder without odour and o

Explain about the micronutrient deficiencies, Explain about the Micronutrie...

Explain about the Micronutrient Deficiencies? Micronutrient deficiencies are more common during calamities. Can you guess, why? This is due to the lack of diversified food item

Spermeiogenesis / spermetoleosis, SPERMEIOGENESI S / SPERMETOLEOSIS It...

SPERMEIOGENESI S / SPERMETOLEOSIS It is the gradual differentiation of a stationary rounded and undifferentiated spermatid into an active and motile sperm. The spermatids f

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd