Define about the column chromatography, Biology

Assignment Help:

Define about the Column chromatography?

In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.

The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.


Related Discussions:- Define about the column chromatography

Structure of the intestines, Describe the structure of the intestines. How ...

Describe the structure of the intestines. How are they modified to carryout their functions? How does the human intestine compare to the fetal pig?

Quantitative characters - population density, Quantitative Characters - Pop...

Quantitative Characters - Population Density Density denotes the average number of individuals of a particular species in a unit area. In other words, it represents the numeri

Cardiac branches and cardiac plexuses, The vagus gives of one superior cerv...

The vagus gives of one superior cervical cardiac branch and an inferior cervical cardiac branch in the neck. These branches descend into the thorax and take part in performing the

Genetic, 1. The genes for ruby eyes (rb), tan body (t) and cut wings (ct) a...

1. The genes for ruby eyes (rb), tan body (t) and cut wings (ct) are all found on the X-chromosome of Drosophila melanogaster. All of these are recessive traits. They map in the or

Explain limited mucosal contact diarrhoea, Q. Explain Limited mucosal conta...

Q. Explain Limited mucosal contact diarrhoea? Limited mucosal contact diarrhoea: It results from situations of inadequate mixing of chyme (semi-liquid mass of food passing thro

PHYLUM PROTOZOA, STATE THE ECONOMIC AND ECOLOGICAL IMPORTANCE OF ECHINODERM...

STATE THE ECONOMIC AND ECOLOGICAL IMPORTANCE OF ECHINODERMS

A healthy potted pitcher plant, A healthy potted pitcher plant when kept in...

A healthy potted pitcher plant when kept in a green house with favourable conditions of light, temperature, soil and water showed poor growth although there was no infection. Give

Use mathematical calculations to justify the answer, Using an average wavel...

Using an average wavelength of 450 nm (0.45 um) can you resolve two objects that sit greater than 200nm apart from one another? Use mathematical calculations to justify your answer

Bjork-shiley valve (b-s)-types of valves, Bjork-Shiley Valve (B-S) : This...

Bjork-Shiley Valve (B-S) : This is one of the earliest types of tilting disc valves introduced in early 70's. The earlier models like concavo-convex disc valves were withdrawn fr

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd