Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
#question.which annelids shws bioluminescence .
Animal Biotechnology: Animal cell cultures have been and are being generate important products based on their genetic information or due to genes transferred into them (transge
is crossing over of chromosomes is a method?
Fatty acids are a very significant adenosine triphosphate (ATP) and source of energy for various cellular organisms. Over fatty acids, glucose and other nutrients can be stored eff
Forests, grasslands and wetland protect landscapes against erosion floods and landslides through the binding action of plant roots. The root systems of plants hold soils in place,
uses and advantages and disadvantages of immunolocalization
Strawberry Slain: It signfies the dilatation of deeper vessels to form cavernous spaces like those seen in the erectile tissue of the penis. The lesion is well-defined
what are the disadvantages of protozoa
Q. Why can the genetic code be qualified as a "degenerate code"? The genetic code is a degenerate code because there are amino acids codified by more than one type of codon, it
What do you understand by Parapodia? Paired lateral, unjointed appendages of polychaete worms. Parapodia have a variety of shapes, and their appearance is related to their nume
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd