Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
Which of the following statements about how B and T cells recognize antigen are true? a. B cells only recognize antigen presented by class I or class II MHC molecules. b. Both cell
The nature of the sounds produced by percussion (tapping) a patient's chest can tell a physician a great deal about the condition of the organs within the thoracic cavity. Healthy,
Q. What are the major human diseases caused by roundworms? The major human diseases caused by nematodes are ancylostomiasis, ascariasis (hookworm infection) and filariasis (com
DISORDER S OF MALE REPRODUCTIVE SYSTEM (i) Prostatomegaly - Enlargment of prostate gland. It causes difficult & painful micturition. (ii) Impotence - Inability of male
Animal Biotechnology: Animal cell cultures have been and are being generate important products based on their genetic information or due to genes transferred into them (transge
Estimation of Depth of Burn Injury: A thermal injury is described as partial thickness or full thickness, depending on the depth and severity of tissue damage a) First D
Q. Various types of microorganisms? Microbes are single-cell organisms so tiny that millions can fit into the eye of a needle. They are the oldest form of life on earth. Micro
How can denaturizing be classified regarding its reversibility? Ans) Protein denaturizing can be a reversible or irreversible process, i.e., it can be possible or impossible to
How do mineral salts participate in osmotic regulation? Osmotic pressure depends on the number of particles dissolved in a solution and not on the nature of such particles. Min
What mode of nutrition did lizard exihbite
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd