Define about the column chromatography, Biology

Assignment Help:

Define about the Column chromatography?

In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.

The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.


Related Discussions:- Define about the column chromatography

How many atp molecules are made after glycolysis, How many ATP molecules ar...

How many ATP molecules are made after glycolysis? Glycolysis is a process same to glucose degradation in fermentation. It forms (final balance) two molecules of ATP for each br

What is a prion, Q. What is a prion? The prion is an infectious (transm...

Q. What is a prion? The prion is an infectious (transmissible) protein able to replicate by transforming other proteins into a copy of the prion. The mechanism of the copying i

What is the group of the phylum chordata, What is the group of the phylum C...

What is the group of the phylum Chordata that first colonized the terrestrial environment? From which habitat did they come? Amphibians, partially aquatic partially terrestrial

Admission of mentally patient, Admission of Mentally Patient: Volunt...

Admission of Mentally Patient: Voluntary Basis  (Voluntary Admission)   Any adult person who considers himself  to be mentally ill, can  request  the Medical Officer for

Agro industrial-copper, Copper Copper is an essential trace element re...

Copper Copper is an essential trace element required for enzyme systems, iron metabolism, connective tissue metabolism, integrity of the central nervous and immune systems. Co

What are the main manifestations of leishmaniasis, Q. What are the main man...

Q. What are the main manifestations of leishmaniasis? There are two key forms of leishmaniasis: visceral leishmaniasis and cutaneous leishmaniasis (also known as kala-azar). Th

State about the neuropsychological assessment, State about the Neuropsychol...

State about the Neuropsychological assessment Neuropsychological assessment provides information concerning the status of brain function across the life span. It does so primar

Describe the nutritional and functional role of manganese, Minerals :- Mang...

Minerals :- Manganese Food Source      Whole grains, fruits, vegetables Nutritional Functional role Essential nutrient: Deficiency extremely rare. Enzyme cofactor

Are mollusc metameric beings, Are mollusc metameric beings? Exclusively ana...

Are mollusc metameric beings? Exclusively analyzing this feature could it be said that molluscs are evolutionarily proximal to nematodes or to annelids? In molluscs there is no

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd