Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
Define osseointegration and its theories. Osseointegration implies that "it is a contact established without interposition of non bony tissue between normal remodeled bone and
Q. How is the light from the sun transformed into chemical energy to be used by the living beings on earth? Light from the sun is converting into chemical energy contained in o
Q. How are the triglycerides made? Triglycerides, oils or fats, are made of one molecule of glycerol bound to three molecules of fatty acids. Hydroxyls of each one of the three
How can amine groups be classified? Amines can be classify into primary amines, those to which one -R (variable radical) is attached to a -NH2, secondary amines, those where on
in what aspect are the cnidarians similar to the protozoans?
Relapse of infective endocarditis usually occurs within two months of the discontinuation of antimicrobial therapy. The relapse rate for patients with native-valve endocarditis cau
The Word 'disaster' is believed to be originating from the French word 'Disastre' which is a combination of two words 'des' meaning bad and 'astre' meaning star. Thus this term ref
what are viruses?its history?its life cycle and the diseases caused by it?
Foot-rot Foot-rot is a term applied to the condition of feet of cattle, sheep, goats and sometimes pigs. It is characterized by inflammation, necrosis and ulceration of underly
what direct or indirect effect is " acid rain" thought to have on (I) lakes, (ii) forests and (iii) buildings
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd