Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define about the Column chromatography?
In column chromatography, a tube is filled with the material constituting the stationary phase, plus a solvent (mobile phase). The general adsorbent (stationary phase) used are alumina, silica gel, activated carbon, calcium phosphate and hydroxyapatite. The substances to be separated are dissolved in the smallest possible volume of a suitable solvent and applied on the top of the stationary phase and allowed to enter the column. The chromatogram is then developed by flowing a solvent (the mobile phase) through the column. As different substances move through the column, they separate and appear in the effluent when particular volumes of liquid have passed through the column. The liquid leaving the column (the eluent) is usually collected as discrete fractions, using an automatic collector.
The separated components are then identified by testing aliquots of each fraction by one or a combination of the following methods viz. colorimetry, UV absorption, fluorimetry, scintillation counting, refractive index, spectral diode array system, electrochemical detection, radioimmunoassay and enzyme immunoassay. When automatic fraction collector is used the recorder automatically draws each peak and the area of each peak is proportional to the amount of sample component present in it. The simplest form of column chromatography is adsorption chromatography. Separation of components by this method depends upon differences both in their degree of adsorption by the adsorbent and solubility in the solvent used for separation. These physiochemical factors are governed by the molecular structure of the compound.
Define Drug effects on Lipid Metabolism? Drugs can affect the metabolism of various essential nutrients in the body. These impairments are highlighted herewith: Lipid metabo
The A and B antigens in humans may be found in water-soluble form in secretions, including saliva, of some individuals (Se/Se and Se/se) but not in others (se/se). The population t
How many parts of human body?
Before we proceed to discuss kin selection we should define the term altruism. It refers to the behaviour pattern of an individual in the population for the benefit of other member
All plants and animals have a natural microflora associated with them. For example, the plants have a natural microflora associated with the surface of roots, stems, leaves e
PHOSPHOLIPIDS Most abundant lipid present in cell membrane, also called membrane lipid. It is made up of lipid & phosphoric acid. The basic phospholipid is phosphat
Determine about the Klove Grooved Pegboard Test The subject must place pegs shaped like keys into a board having recesses that are oriented in randomly varying directions. The
what re conjugated proteins and how are they classified?
Q. What are the hormones that promote the release of the female gamete from the follicle and at which day of the menstrual cycle does this phenomenon happen? What is this event cal
Alimentary tract The alimentary tract and the secretary organs associated with it are involved in the process of digestion. The sequence of events includes ingestion of food, d
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd