culturing, Biology

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ime day 0 day 1 day 3 day 4 day 5 day 7

count

blank 7 10 5 14 9 8

low 45&49 64&70 62&69 75&79 80&82 68&72

high 45&49 72&78 100&112 180&195 250&267 208&226

Calculate the cell yield for the following data that were obtained using a coulter counter. Conditions:

machine counting volume: 0.5 mL
PBS in Vial: 15.0 mL
Sample added: 0.2 mL
Total sample size:
Day 0: 1.0 mL
Day 1: 1.0 mL
Day 3: 2.0 mL
Day 4: 2.0 mL
Day 5: 2.5 mL
Day 7: 3.0 mL

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