Culture conditions, Biology

Assignment Help:

Culture Conditions

The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of light and temperature. If possible the relative humidity should not fall below 50% to prevent rapid desiccation of the medium. To minimize the infection of cultures during incubation, the culture room should be dust free. It is often provided with double doors and entry of people is restricted. The requirement for light and temperature varies considerably. The cultures of high altitude plants as well as those of desert species grow well at 25? c-28?c. However, induction of pollen embryogenesis requires a high temperature treatment (35? C). As mentioned earlier, in cultures even green tissues and shoots do not exhibit active photosynthesis and are largely heterotrophic. The differentiation of shoots from callus or explants and axillary shoot proliferation are favored by light.

A callus is defined as an unrecognized mass of tissue varying widely in texture, appearance and growth rate. Such cultures should be maintained in continuous diffuse light (1-3K lux) provided by fluorescent tubes. You should know that unorganized callus and freshly isolated protoplast cultures are often sensitive to light and are incubated in darkness. Total darkness is also favorable for rooting and for the initial stages of pollen and somatic embryogenesis.


Related Discussions:- Culture conditions

Define about the probiotics, Define about the Probiotics? Dietary Sourc...

Define about the Probiotics? Dietary Sources and their Mode of Action/Effects We can have specific preparations designed for probiotic use, as well as, foods. Probiotic product

Contra indication in circulatory assist devices, Contra Indication :  It i...

Contra Indication :  It is absolutely contra indicated if their is more than trivial aortic regurgitation. Aortic aneurysm and severe aorto iliac disease are also contra indicatio

What er the parafunctional habits, What er the Parafunctional Habits P...

What er the Parafunctional Habits Parafunctional Habits like bruxism and clenching create mechanical and biological problems due to overloading and are considered to be one of

Particular variety of lettuce seed, How would you design, in principle, an ...

How would you design, in principle, an experiment to test the hypothesis that a particular variety of lettuce seed required daylight in order to germinate   You would requi

Polygonum type - monosporic embryo sacs, Polygonum Type - Monosporic Embryo...

Polygonum Type - Monosporic Embryo Sacs The embryo sac is formed from the chalazal megaspore in the tetrad and is eight-nucleate. The development of the embryo sac begins with

How to use subcellular fractionation and a western blot, A virus capsid con...

A virus capsid containing vimentin, your protein of interest, gets into the cell nucleus and is broken down with the DNA being released. It is unknown whether the capsids themselve

How to know what i already paid, I know the answer to the question but when...

I know the answer to the question but when I selected buy it said $80, now I want to know if it is $80 more or if you will subtract what I already paid to $80 and therefore will on

Age-sex distribution, Age-Sex Distribution While studying exponential...

Age-Sex Distribution While studying exponential growth we need to know more than just the birth and death rates because these rates vary with age and sex. Age structure in a

Push the food down through the esophagus, Q. Which is the kind of muscle ti...

Q. Which is the kind of muscle tissue that helps to push the food down through the esophagus? The esophageal wall in its superior portion is made of skeletal striated muscle th

What is replication, Question 1 Write a short note on the following- ...

Question 1 Write a short note on the following- Structure of DNA. Satellite DNA Transcription. pBR 322 vector Cytokines Microarrays Question 2 What is

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd