Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Culture Conditions
The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of light and temperature. If possible the relative humidity should not fall below 50% to prevent rapid desiccation of the medium. To minimize the infection of cultures during incubation, the culture room should be dust free. It is often provided with double doors and entry of people is restricted. The requirement for light and temperature varies considerably. The cultures of high altitude plants as well as those of desert species grow well at 25? c-28?c. However, induction of pollen embryogenesis requires a high temperature treatment (35? C). As mentioned earlier, in cultures even green tissues and shoots do not exhibit active photosynthesis and are largely heterotrophic. The differentiation of shoots from callus or explants and axillary shoot proliferation are favored by light.
A callus is defined as an unrecognized mass of tissue varying widely in texture, appearance and growth rate. Such cultures should be maintained in continuous diffuse light (1-3K lux) provided by fluorescent tubes. You should know that unorganized callus and freshly isolated protoplast cultures are often sensitive to light and are incubated in darkness. Total darkness is also favorable for rooting and for the initial stages of pollen and somatic embryogenesis.
Name an artificial fertiliser or fertilisers which farmers can use to increase the supply of nitrate, phosphate and potassium to their crops. NPK compound fertiliser havin
Tina administered 1 liter of sterile distilled water IV to a patient. Predict the direction (increase, decrease, no change) you would expect Tina's infusion to have produced in the
Explain Whey protein concentrates Whey protein concentrates (WPC) are the products derived from whey from which the water, minerals and lactose have been removed. WPC is a wh
Before Purchasing Ideally it's wise to buy a readymade software with options to further customize it to suit your managerial functions. A totally Custom-made softwar
Sex Determination - Reproduction Sex, whether an individual will be a male or a female is determined at fertilisation, and this directs and controls all the later processes i
Maturation and adaptation: (18 to 54 weeks) The final stage involves maturation and adaptation of the implant-bone interface, peri-implant bone and the entire implant supportin
I nfectious bronchitis (IB) Infectious bronchitis is a highly contagious respiratory disease of poultry, caused by a virus belonging to the family Coronaviridae. Its existence
WHAT ARE THE DIFFERENT CULTURE MEDIAS USED FOR ISOLATION OF ACTINOMYCETES?
Collection of Cerebro-spinal Fluid (CSF) Examination of the CSF is one of the most useful laboratory procedures in neurological diagnosis. Four types of punctures ma
Q. Explain Injection Rate and Volume? This is best achieved by injecting directly into the ventricular chamber. Midcavitary position of the catheter ensures that there is no ve
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd