Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Culture Conditions
The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of light and temperature. If possible the relative humidity should not fall below 50% to prevent rapid desiccation of the medium. To minimize the infection of cultures during incubation, the culture room should be dust free. It is often provided with double doors and entry of people is restricted. The requirement for light and temperature varies considerably. The cultures of high altitude plants as well as those of desert species grow well at 25? c-28?c. However, induction of pollen embryogenesis requires a high temperature treatment (35? C). As mentioned earlier, in cultures even green tissues and shoots do not exhibit active photosynthesis and are largely heterotrophic. The differentiation of shoots from callus or explants and axillary shoot proliferation are favored by light.
A callus is defined as an unrecognized mass of tissue varying widely in texture, appearance and growth rate. Such cultures should be maintained in continuous diffuse light (1-3K lux) provided by fluorescent tubes. You should know that unorganized callus and freshly isolated protoplast cultures are often sensitive to light and are incubated in darkness. Total darkness is also favorable for rooting and for the initial stages of pollen and somatic embryogenesis.
Explain the Scanning Electron Microscope It can be used to observe intact cells or cell components directly. Then sections are not necessary. It is used for visualizing surface
1. Transcriptional analyses of eukaryotic cells reveal widespread production of RNA. Using specific examples describe how: a) microRNAs are able to influence gene expression.
Explain Defensive Proteins These proteins protect against diseases. Antibodies are the example of defensive proteins. These combat bacteria and viruses. Also, immunoglobulins,
What is the best microscope to get a detailed view of the parts inside of a preserved plant cell
Q. What is diabetes mellitus? Diabetes mellitus is the disease caused by action or deficient production of insulin and the consequent high blood glucose level and low glucose u
Ask question #Minimum 100 words adccepted#
Define Water - Important Nutrient? Macronutrients, you know, are those nutrients which are required in large amounts by our body namely, carbohydrates, fats and proteins. We st
Pathogenic bacteria Heterotrophic bacteria could be divided into saprophytes and parasites. Saprophytes normally do not cause any disease and they survive on dead or decaying
Q. What is self-catalytic RNAs? Ribozymes - Term 'ribozyme' was initially suggested by Thomas R. Cech, Nobel Prize winning biochemist, who discovered this class of RNA molecul
What are the main factors that alter the speed of enzymatic reactions? The major factors that change the speed of enzymatic reactions are temperature, pH and substrate concentr
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd