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Culture Conditions
The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of light and temperature. If possible the relative humidity should not fall below 50% to prevent rapid desiccation of the medium. To minimize the infection of cultures during incubation, the culture room should be dust free. It is often provided with double doors and entry of people is restricted. The requirement for light and temperature varies considerably. The cultures of high altitude plants as well as those of desert species grow well at 25? c-28?c. However, induction of pollen embryogenesis requires a high temperature treatment (35? C). As mentioned earlier, in cultures even green tissues and shoots do not exhibit active photosynthesis and are largely heterotrophic. The differentiation of shoots from callus or explants and axillary shoot proliferation are favored by light.
A callus is defined as an unrecognized mass of tissue varying widely in texture, appearance and growth rate. Such cultures should be maintained in continuous diffuse light (1-3K lux) provided by fluorescent tubes. You should know that unorganized callus and freshly isolated protoplast cultures are often sensitive to light and are incubated in darkness. Total darkness is also favorable for rooting and for the initial stages of pollen and somatic embryogenesis.
Vitamin D (Calciferol - vitamin D2) The main forms are Vitamin D2 (ergocalciferol-plant origin) and vitamin D3 (cholecalciferol-animal origin). Vitamin D2 forms colourless, aci
Hybridization is the reaction by which pairing of the complementary strands of nucleic acid happens. DNA is usually double-stranded, and when these strands are separated form each
define the pattern of cleavage i all aspects
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Successive Changes in Animal Life during Hydrosere The successive changes in plant communities in the different seral communities of a hydrosere. The question arises, is ther
Pathogenesis The mode of intracellular replication of chlamydial agents is investigated morphologically and cytochemically with both light and electron microscope. It is estab
What factors lead to spoilage of fish flesh? A difference in the composition of tissues among different species, climate, procurement and holding practices are amongst few of
Q. What is the role of the ozone layer for living beings? The Ozone, O3, is a gas of the atmosphere that filters ultraviolet radiation from the sun disallowing most of that rad
TYPES OF CLEAVAGE - Depending upon the distribution & amount of yolk, cleavage is of following types - 1 . HOLOBLASTIC EQUAL CLEAVAGE ( holos , whole ; blastos, ger
Name the source gland of leutinising hormone (LH). State the other hormone along with which it acts on its target cells/organ. Give their two functions.
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