Culture conditions, Biology

Assignment Help:

Culture Conditions

The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of light and temperature. If possible the relative humidity should not fall below 50% to prevent rapid desiccation of the medium. To minimize the infection of cultures during incubation, the culture room should be dust free. It is often provided with double doors and entry of people is restricted. The requirement for light and temperature varies considerably. The cultures of high altitude plants as well as those of desert species grow well at 25? c-28?c. However, induction of pollen embryogenesis requires a high temperature treatment (35? C). As mentioned earlier, in cultures even green tissues and shoots do not exhibit active photosynthesis and are largely heterotrophic. The differentiation of shoots from callus or explants and axillary shoot proliferation are favored by light.

A callus is defined as an unrecognized mass of tissue varying widely in texture, appearance and growth rate. Such cultures should be maintained in continuous diffuse light (1-3K lux) provided by fluorescent tubes. You should know that unorganized callus and freshly isolated protoplast cultures are often sensitive to light and are incubated in darkness. Total darkness is also favorable for rooting and for the initial stages of pollen and somatic embryogenesis.


Related Discussions:- Culture conditions

How does the amoeboid movement occur, How does the amoeboid movement occur?...

How does the amoeboid movement occur? What are examples of beings and cells that use such movements for locomotion? Amoeboid movements are formed by cytoplasmic movements and p

Diverse proteins made by the same total number of amino acid, Q. Are there ...

Q. Are there diverse proteins made by the same total number of amino acids? Diverse proteins with the same total number of amino acids may exist. In such cases the discriminati

Define nutritional requirements for extreme environments, Define Nutritiona...

Define Nutritional Requirements for Extreme Environments? Human beings have been able to survive and work under extreme environments of almost all regions of the earth, from po

Explain how movement is controlled in protozoans., Movement is even control...

Movement is even controlled by organelles, cilia and flagella. These and the pseudopodia in the amoebas give these small creatures movement, something we associate with animals. Es

Why is the fish circulation classified as simple circulation, Why is the fi...

Why is the fish circulation classified as a simple and complete circulation? Complete circulation is that in which there is no mixture of venous blood and arterial blood. As ci

Determine the duodenum from the stomach called, What is the valve that sepa...

What is the valve that separates? What is its function? The valve that divides the stomach from the duodenum is the pylorus. It has the function of keeping the food bolus within

Density - population parameters and regulation, Density - Population Parame...

Density - Population Parameters and Regulation Density is defined as number of individuals or population biomass per unit of area or volume at any given time. Biomass refers t

Classification based on digestive fate of carbohydrates, Explain Classifica...

Explain Classification Based on Digestive Fate of Carbohydrates? The digestive fate of carbohydrates depends on their inherent chemical nature (monosaccharide composition and t

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd