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Culture Conditions
The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of light and temperature. If possible the relative humidity should not fall below 50% to prevent rapid desiccation of the medium. To minimize the infection of cultures during incubation, the culture room should be dust free. It is often provided with double doors and entry of people is restricted. The requirement for light and temperature varies considerably. The cultures of high altitude plants as well as those of desert species grow well at 25? c-28?c. However, induction of pollen embryogenesis requires a high temperature treatment (35? C). As mentioned earlier, in cultures even green tissues and shoots do not exhibit active photosynthesis and are largely heterotrophic. The differentiation of shoots from callus or explants and axillary shoot proliferation are favored by light.
A callus is defined as an unrecognized mass of tissue varying widely in texture, appearance and growth rate. Such cultures should be maintained in continuous diffuse light (1-3K lux) provided by fluorescent tubes. You should know that unorganized callus and freshly isolated protoplast cultures are often sensitive to light and are incubated in darkness. Total darkness is also favorable for rooting and for the initial stages of pollen and somatic embryogenesis.
It is defined as the presence of chemicals and particulate in the atmosphere in quantities and pollution that are harmful to human health and environment. It occurs when the concen
Food Applications of Agar The bakery industry has been the largest user of agar because of its heat- resistant gel properties. Confectionary products, such as agar jelly candi
Which of the following best defines why the two DNA polymerase proteins that are held by the sliding clamp are oriented in opposite directions? A. The efficiency of replication
Each of the properties that follow is characteristic of the carbon atom. In each case, indicate how the property contributes to the role of the carbon atom as the most important at
State the types of bone density Misch classified bone density into following types (1988) D1. Dense cortical bone- which is almost never observed in maxilla and approximatel
Baculovirus is widely used for expressing recombinant proteins. Structural of baculovirus is a rod-shaped nucleocapsid containing genomic DNA. The baculovirus Autographa California
1. Noise producing industries, railway stations, and aerodromes should be located away from the residential areas. 2. Check on the traffic in the residential colonies.
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TYPES OF BLASTULA - Blastulas are of following type - 1 . STEREOBLASTULA - The blastocoel is very small. It's blastomere are less in num
Q. What is vision? Why is vision important for life on earth? Vision is the ability of some living beings to distinguish, to perceive and to interpret luminous stimuli. Visi
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