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Culture Conditions
The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of light and temperature. If possible the relative humidity should not fall below 50% to prevent rapid desiccation of the medium. To minimize the infection of cultures during incubation, the culture room should be dust free. It is often provided with double doors and entry of people is restricted. The requirement for light and temperature varies considerably. The cultures of high altitude plants as well as those of desert species grow well at 25? c-28?c. However, induction of pollen embryogenesis requires a high temperature treatment (35? C). As mentioned earlier, in cultures even green tissues and shoots do not exhibit active photosynthesis and are largely heterotrophic. The differentiation of shoots from callus or explants and axillary shoot proliferation are favored by light.
A callus is defined as an unrecognized mass of tissue varying widely in texture, appearance and growth rate. Such cultures should be maintained in continuous diffuse light (1-3K lux) provided by fluorescent tubes. You should know that unorganized callus and freshly isolated protoplast cultures are often sensitive to light and are incubated in darkness. Total darkness is also favorable for rooting and for the initial stages of pollen and somatic embryogenesis.
excretary organ of whale
Define the Streak Plate Method - Pure Culture Techniques? The method was developed by Loeffler and Gaffkey in the laboratory of Robert Koch. The streak plate method depends on
Q. Why are the salt and sugar used in the production of dried fruits and dried meat? Substances that sustain a highly hypertonic environment, like salt and sugar, are used in t
What are the phytoplankton and the zooplankton? Phytoplankton and zooplankton are parts of the plankton. The phytoplankton comprises the autotrophic floating beings: cyanobacte
Q. Patient care plan for hypertensives? - Lifestyle changes: Avoiding smoking, use of tobacco, and excess alcohol intake Physical activity like walking, 4 times a week or 40 mi
Q. What are the gynoecium and the androecium? What are the other structures of flowers? Androecium is set of male reproductive structures of flowers it comprehends the stamens
compare and contrast the structure of the following: hyrda plain, hydra mematocysts, obelia, amoeba, euglena, paramecium caudatum conjugation, euglena acus.
What are some of the presumed target substrates for cyclin-dependent kinases?
he heartbeat begins with the depolarization of the
Which kind of plant tissue is cork? The Cork is the material for instance, used to cap wine bottles, and is extracted from the suber of a special oak called cork.
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