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Culture Conditions
The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of light and temperature. If possible the relative humidity should not fall below 50% to prevent rapid desiccation of the medium. To minimize the infection of cultures during incubation, the culture room should be dust free. It is often provided with double doors and entry of people is restricted. The requirement for light and temperature varies considerably. The cultures of high altitude plants as well as those of desert species grow well at 25? c-28?c. However, induction of pollen embryogenesis requires a high temperature treatment (35? C). As mentioned earlier, in cultures even green tissues and shoots do not exhibit active photosynthesis and are largely heterotrophic. The differentiation of shoots from callus or explants and axillary shoot proliferation are favored by light.
A callus is defined as an unrecognized mass of tissue varying widely in texture, appearance and growth rate. Such cultures should be maintained in continuous diffuse light (1-3K lux) provided by fluorescent tubes. You should know that unorganized callus and freshly isolated protoplast cultures are often sensitive to light and are incubated in darkness. Total darkness is also favorable for rooting and for the initial stages of pollen and somatic embryogenesis.
When an individual is exposed to foreign serum antigen then a combination of symptoms are produced which is known as serum sickness.
Explain what is Prosthetic Valve Endocarditis (PVE) ? Prosthetic valve endocarditis (PVE) is called EARLY when symptoms begin within 60 days of surgery and LATE when the onset
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Do moulds grow better where it is dark or light? This time leave one culture dish in a warm place where it receives light all the time. Place the other dish in a warm dark plac
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How many of the same proteins are made at the same time by each ribosome in the translation of one mRNA molecule? How does consecutive protein production occur in translation?
Enzymes utilization in food industry Enzymes may be used in industry as components of living cells or after isolation in free or immobilized forms. All of them may be referred
What does acute disease mean? An sharp disease is which does not last for a longer duration n has no weakness in the body
Explain the Life History of Lycophytes? Lycophytes have two separate and distinct generations in their life history that alternate with each other. The gametophyte stage is tin
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