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Culture Conditions
The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of light and temperature. If possible the relative humidity should not fall below 50% to prevent rapid desiccation of the medium. To minimize the infection of cultures during incubation, the culture room should be dust free. It is often provided with double doors and entry of people is restricted. The requirement for light and temperature varies considerably. The cultures of high altitude plants as well as those of desert species grow well at 25? c-28?c. However, induction of pollen embryogenesis requires a high temperature treatment (35? C). As mentioned earlier, in cultures even green tissues and shoots do not exhibit active photosynthesis and are largely heterotrophic. The differentiation of shoots from callus or explants and axillary shoot proliferation are favored by light.
A callus is defined as an unrecognized mass of tissue varying widely in texture, appearance and growth rate. Such cultures should be maintained in continuous diffuse light (1-3K lux) provided by fluorescent tubes. You should know that unorganized callus and freshly isolated protoplast cultures are often sensitive to light and are incubated in darkness. Total darkness is also favorable for rooting and for the initial stages of pollen and somatic embryogenesis.
M-Mode was the earliest form of cardiac ultrasound used clinically. It displays the amplitude signals of various structures along the length of ultrasound beam with distance or
A woman who is heterozygous for a particular X-linked recessive trait marries a phenotypically normal man. What percentage of their sons will show the recessive phenotype?
charecterstic features of phylum protozoa.
POST-OPERATIVE CARE OF PATIENTS The first few days following cardiac operations are the most critical in terms of the patient's survival. The safety with which a patient can
I stored a few of my things from April to September this year. When I wanted to unpack the boxes, I found hundreds of round, small, black things. They look like they might be snail
Phylum Ciliophora 1) They have cilia at some stage in the life-cycle. The cilia are used for locomotion or creating a feeding current. 2) They feed heterotrophically usually
α - amylase activity by GA 3 - Dormancy Of the enzymes required for the digestion of starch α-amylase appears immediately after the start of germination. It was found that
differentiate between scanning electron microscope and a transmission electron microscope in terms of their functions
Define Estimation of Protein by Growth and body, weight changes? The methods under this category include Protein efficiency ratio (PER) Net protein ratio (NPR)
Animals of the open water zone The limnetic region of this zone contains certain fishes as well as rotifers, zooplankton such as crustacean and protozoan. In the profundal zo
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