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Culture Conditions
The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of light and temperature. If possible the relative humidity should not fall below 50% to prevent rapid desiccation of the medium. To minimize the infection of cultures during incubation, the culture room should be dust free. It is often provided with double doors and entry of people is restricted. The requirement for light and temperature varies considerably. The cultures of high altitude plants as well as those of desert species grow well at 25? c-28?c. However, induction of pollen embryogenesis requires a high temperature treatment (35? C). As mentioned earlier, in cultures even green tissues and shoots do not exhibit active photosynthesis and are largely heterotrophic. The differentiation of shoots from callus or explants and axillary shoot proliferation are favored by light.
A callus is defined as an unrecognized mass of tissue varying widely in texture, appearance and growth rate. Such cultures should be maintained in continuous diffuse light (1-3K lux) provided by fluorescent tubes. You should know that unorganized callus and freshly isolated protoplast cultures are often sensitive to light and are incubated in darkness. Total darkness is also favorable for rooting and for the initial stages of pollen and somatic embryogenesis.
What raw materials require for the manufacture of margarine Soybean and peanut (or groundnut) oils are of great economic importance. Refined soybean oil contains branched fura
Q. What are the effects of somatostatin for the pancreatic hormonal secretion? Somatostatin inhibits both glucagon and insulin secretions.
VACUOLES They are of 4 types - 1. Sap Vacuole - Outer membrane of sap vacuole is called T onoplast . Sap vacuole contains amino sugars, carotenoids, proteins, m
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What is crossing over? How is meiosis related to this phenomenon? The Linked alleles for instance, A-b and a- B form the gametes A-b and a-B that maintain the linkage of the al
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what is respiration?
What is the life cycle of the gymnosperms? As all plants they show a diplobiontic life cycle, i.e., alternation of generations with diploid and haploid stages. The lasting (fin
Explain about the Serum vitamin B12 assay? Serum vitamin B 12 assay: The vitamin B 12 content of serum can be determined. A serum level of 12 deficiency.
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