Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Culture Conditions
The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of light and temperature. If possible the relative humidity should not fall below 50% to prevent rapid desiccation of the medium. To minimize the infection of cultures during incubation, the culture room should be dust free. It is often provided with double doors and entry of people is restricted. The requirement for light and temperature varies considerably. The cultures of high altitude plants as well as those of desert species grow well at 25? c-28?c. However, induction of pollen embryogenesis requires a high temperature treatment (35? C). As mentioned earlier, in cultures even green tissues and shoots do not exhibit active photosynthesis and are largely heterotrophic. The differentiation of shoots from callus or explants and axillary shoot proliferation are favored by light.
A callus is defined as an unrecognized mass of tissue varying widely in texture, appearance and growth rate. Such cultures should be maintained in continuous diffuse light (1-3K lux) provided by fluorescent tubes. You should know that unorganized callus and freshly isolated protoplast cultures are often sensitive to light and are incubated in darkness. Total darkness is also favorable for rooting and for the initial stages of pollen and somatic embryogenesis.
Q. Does the fish heart pump venous or arterial blood? After oxygenation in the gills the arterial blood goes to the tissues so the fish heart pumps venous blood, the venous blo
Phosphofiuctokinase-I Phosphofiuctokinase-I is activated by AMP and inhibited by ATP and citrate. When ATP is utilized in energy requiring process, the concentration ofAMP
simpson''s index
What is tubal pregnancy? Many times fecundation takes place in the Fallopian tubes. Usually the newly formed zygote is taken to the uterus where nidation and the embryonic deve
Limiting Factor - Ecosystem In all ecosystems one factor, usually abiotic, limits the growth of organisms and is therefore called a limiting factor. The limiting factor is one
Explain Precautions for Estimation of Sucrose by Fehling Soxhlet Method? 1. Maintain proper temperature of the water bath for complete hydrolysis. 2. Add distilled water to
Explain Understanding the response of farmers? Understanding the response of the farmers by the government also influences the food production. Government policy influences the
Define Role in growth and cellular Differentiation? The growth and differentiation of epithelial cells throughout the body are especially affected by vitamin A deficiency. In
Explain the working of Skeletal Muscle? Skeletal muscle is under voluntary control and is responsible for voluntary movements of body parts. Skeletal muscle is also called stri
Ur o l it h i a s i s The presence of calculi (urolith) in the urinary system is termed as urolithiasis, and the disease is characterized by distension of bladder and
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd