Culture conditions, Biology

Assignment Help:

Culture Conditions

The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of light and temperature. If possible the relative humidity should not fall below 50% to prevent rapid desiccation of the medium. To minimize the infection of cultures during incubation, the culture room should be dust free. It is often provided with double doors and entry of people is restricted. The requirement for light and temperature varies considerably. The cultures of high altitude plants as well as those of desert species grow well at 25? c-28?c. However, induction of pollen embryogenesis requires a high temperature treatment (35? C). As mentioned earlier, in cultures even green tissues and shoots do not exhibit active photosynthesis and are largely heterotrophic. The differentiation of shoots from callus or explants and axillary shoot proliferation are favored by light.

A callus is defined as an unrecognized mass of tissue varying widely in texture, appearance and growth rate. Such cultures should be maintained in continuous diffuse light (1-3K lux) provided by fluorescent tubes. You should know that unorganized callus and freshly isolated protoplast cultures are often sensitive to light and are incubated in darkness. Total darkness is also favorable for rooting and for the initial stages of pollen and somatic embryogenesis.


Related Discussions:- Culture conditions

Define role of leptin in controlling gene expression, Define role of Leptin...

Define role of Leptin in controlling gene expression? Leptin is a protein product (containing 167 amino acids) of the ob gene expressed exclusively in the adipose tissue. This

What are uses of hydrocolloids for health, Uses of Hydrocolloidsfor health ...

Uses of Hydrocolloidsfor health Hydrocolloids, together with other dietary fibres are increasingly being seen as contributing to a number of health effects. Some of the hydroco

Determine the atomic number of element, A.    In order to determine the ato...

A.    In order to determine the atomic number of an element we need to know the number of protons in an atom. As we know that atom is neutral we can also take the number of electro

The difference between high and low c, How does brain recognize difference ...

How does brain recognize difference between high and low c and soft and loud sounds?

Explain uncompetitive inhibition, Uncompetitive inhibition  In this typ...

Uncompetitive inhibition  In this type of inhibition, the inhibitor only binds with the enzyme-substrate complex making  it  inactive.  As  a  result,  the  product formation

What is catharanthus roseus pharmaceutical compounds, Q. What is Catharanth...

Q. What is Catharanthus roseus pharmaceutical compounds? This species yielded the alkaloids vincristine and vinblastine. Traditionally used by various cultures for the treatmen

Types of protein fibers of the connective tissue proper, Q. What are the th...

Q. What are the three kinds of protein fibers of the connective tissue proper? The matrix of the connective tissue proper is made of elastic fibers, collagen fibers and reticul

Explain the obesity - problems of older children nutrition, Explain the Obe...

Explain the Obesity - Problems of Older Children and Adolescent Nutrition? One of the major problems is raising obesity rates (upto 30%) in urban, well-to-do school children.Th

Determine the eukaryotic replication origins, Which of the following statem...

Which of the following statements is wrong regarding eukaryotic replication origins? A. More origins are licensed and initiated within embryonic cells than in adult cells. B

Slow moving waters - biota of rivers, Slow Moving Waters - Biota of Rivers ...

Slow Moving Waters - Biota of Rivers The habitat of a slowly moving part of the river is very different from the one just described. Here the water flow is comparatively slow

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd