Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Culture Conditions
The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of light and temperature. If possible the relative humidity should not fall below 50% to prevent rapid desiccation of the medium. To minimize the infection of cultures during incubation, the culture room should be dust free. It is often provided with double doors and entry of people is restricted. The requirement for light and temperature varies considerably. The cultures of high altitude plants as well as those of desert species grow well at 25? c-28?c. However, induction of pollen embryogenesis requires a high temperature treatment (35? C). As mentioned earlier, in cultures even green tissues and shoots do not exhibit active photosynthesis and are largely heterotrophic. The differentiation of shoots from callus or explants and axillary shoot proliferation are favored by light.
A callus is defined as an unrecognized mass of tissue varying widely in texture, appearance and growth rate. Such cultures should be maintained in continuous diffuse light (1-3K lux) provided by fluorescent tubes. You should know that unorganized callus and freshly isolated protoplast cultures are often sensitive to light and are incubated in darkness. Total darkness is also favorable for rooting and for the initial stages of pollen and somatic embryogenesis.
Q. Besides the liver which is the other adnexal gland of the digestive system that releases substances in the duodenum participating in extracellular digestion? The other adnex
Mechanical Properties of Ti and Ti Alloy as Compared to Bone Titanium tends to seize when in sliding contact with itself or other metal. Titanium based alloys have a high co-e
Q. What is Colony Collapse Disorder? Bee colonies are attacked by a variety of pathogens and parasites. One major pathogen is the acute bee paralysis virus (IAPV), which cau
respiration in animals
Explain Nevirapine and its adverse effects Nevirapine (NVP,Viramune) - Nevirapine is most effective at raising CD4 cell counts and lowering viral load when combined with 2 NRTI
Structure of the Conduction System of the Heart Sinoatrial (SA) Node is the pacemaker node located in the right atrium near the opening of superior venacava. It is called
Q. Illustrate Omega Taxonomy? After synthesising a basic classification, the taxonomist can attempt to improve upon it. Therefore, the observed facts are interpreted in Omega t
Explain the Process of Solubility test? This test is given by all monosaccharides and disaccharides. Polysaccharides are not soluble in cold water. Principle All monosa
ENZYMES IN CLINICAL DIAGNOSIS The rationale for measuring plasma or serum enzyme levels is based on the premise that these levels reflect changes that have occurred in a
Bone grafting issues Planning of the case is very critical. Any bone defect in the aesthetic zone should be evaluated prior to implant placement in order to obtain a prosthetic
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd