Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Culture Conditions
The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of light and temperature. If possible the relative humidity should not fall below 50% to prevent rapid desiccation of the medium. To minimize the infection of cultures during incubation, the culture room should be dust free. It is often provided with double doors and entry of people is restricted. The requirement for light and temperature varies considerably. The cultures of high altitude plants as well as those of desert species grow well at 25? c-28?c. However, induction of pollen embryogenesis requires a high temperature treatment (35? C). As mentioned earlier, in cultures even green tissues and shoots do not exhibit active photosynthesis and are largely heterotrophic. The differentiation of shoots from callus or explants and axillary shoot proliferation are favored by light.
A callus is defined as an unrecognized mass of tissue varying widely in texture, appearance and growth rate. Such cultures should be maintained in continuous diffuse light (1-3K lux) provided by fluorescent tubes. You should know that unorganized callus and freshly isolated protoplast cultures are often sensitive to light and are incubated in darkness. Total darkness is also favorable for rooting and for the initial stages of pollen and somatic embryogenesis.
Q. Write the meaning of diabetes mellitus? The word "diabetes" is from the Greek word meaning "a siphon". Diabetes patients had polyuria (passing excessive urine) and "pass it
What are some diseases caused by abnormal GH secretion by the hypophysis? In childhood deficient GH secretion might be lead to delayed growth and in severe cases to nanism (dwa
Pre-Diabetes is a condition where blood sugar level is above normal but below the value to be diagnosed as diabetes mellitus. It has been found that those people with blood sugar v
Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4
What is the Importance of Vitamin K The site of action of vitamin K activity is the highly complex mechanism of blood coagulation. Due to its effect on prothrombin, vitamin K i
How can we calculate the IVI from a total number of (30) quadrats (each 30 x 30 m quadrats)
What is the best microscope to get a detailed view of the parts inside of a preserved plant cell
What is biotechnology? Biotechnology is the application of biological knowledge to get new techniques, materials and compounds of pharmaceutical, medical, agrarian, industrial
Explain the types of care of teeths a) Plastic/ Nylon Scalers: These are available in a variety of shapes and are moderately effective in calculus removal. Can be Ultrasonic
Ask question #Minimuwhat are the similarities between pisces and amphibians
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd