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Culture Conditions
The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of light and temperature. If possible the relative humidity should not fall below 50% to prevent rapid desiccation of the medium. To minimize the infection of cultures during incubation, the culture room should be dust free. It is often provided with double doors and entry of people is restricted. The requirement for light and temperature varies considerably. The cultures of high altitude plants as well as those of desert species grow well at 25? c-28?c. However, induction of pollen embryogenesis requires a high temperature treatment (35? C). As mentioned earlier, in cultures even green tissues and shoots do not exhibit active photosynthesis and are largely heterotrophic. The differentiation of shoots from callus or explants and axillary shoot proliferation are favored by light.
A callus is defined as an unrecognized mass of tissue varying widely in texture, appearance and growth rate. Such cultures should be maintained in continuous diffuse light (1-3K lux) provided by fluorescent tubes. You should know that unorganized callus and freshly isolated protoplast cultures are often sensitive to light and are incubated in darkness. Total darkness is also favorable for rooting and for the initial stages of pollen and somatic embryogenesis.
Which statement below about asexual reproduction is FALSE? a.With asexual reproduction, offspring are genetically identical to the parent. b.Asexual reproduction requires no
Define the Need for classification of Plants and Animals? First of all there is a need to know what classification is? Let us define in simple term. Classification is placing o
Cytosketal Mechanism There are two co-ordinate processes in the cleavage (or cell division) that is displayed in Figure. Karyokinesis The mitotic division of nucleus
THE CALORIE VALUE - The amount of heat liberated from complete combusion of 1gm food in a bomb caloriemeter (a closed metal chamber filled with O ) is its gross calorific
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Treatment of diarrhea Loperamide (Imodium, and others), an over-the-counter synthetic opioid (4-mg loading dose, then 2 mg orally after each loose stool to a maximum of 16 mg/d
Q. What are the problems that vertebrates needed to solve to adapt to the terrestrial environment since they came from the aquatic habitat? How evolution does solve those problems?
What happens to respiratory system in a man going up a hill?
Ventilation of Tracheal System The manner in which tracheae are ventilated varies with species of insects but movement of air is achieved in two ways: Diffusion
Explain the Composition of MacConkey Agar? Bactopeptone - 17.0 gm Protease Peptone - 3.0 gm Lactose - 10.0 gm Bile salt mixture - 1.5 gm Sodium chloride - 5.0 gm
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