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Culture Conditions
The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of light and temperature. If possible the relative humidity should not fall below 50% to prevent rapid desiccation of the medium. To minimize the infection of cultures during incubation, the culture room should be dust free. It is often provided with double doors and entry of people is restricted. The requirement for light and temperature varies considerably. The cultures of high altitude plants as well as those of desert species grow well at 25? c-28?c. However, induction of pollen embryogenesis requires a high temperature treatment (35? C). As mentioned earlier, in cultures even green tissues and shoots do not exhibit active photosynthesis and are largely heterotrophic. The differentiation of shoots from callus or explants and axillary shoot proliferation are favored by light.
A callus is defined as an unrecognized mass of tissue varying widely in texture, appearance and growth rate. Such cultures should be maintained in continuous diffuse light (1-3K lux) provided by fluorescent tubes. You should know that unorganized callus and freshly isolated protoplast cultures are often sensitive to light and are incubated in darkness. Total darkness is also favorable for rooting and for the initial stages of pollen and somatic embryogenesis.
Explain Viscosity of Gastrointestinal Contents Delay Absorption? 1. Inadequate mixing of luminal contents due to increased viscosity may slow the movement of digestive enzymes
A dominant allele will always increase in frequency over time. Yes No Q44. When a gene has two alleles, the allele frequencies in a population automatically move toward an equilibr
Tall (T) plants are dominant and short (t) plants are recessive. Two heterozygous tall plants are crossed. What percentage of the offspring will be tall?
what is meant by solitary
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Radial Artery (RA) : Patency of RA is less than that of Ih4.A but much superior to a venous graft. One of the studies showed 90 per cent patency at 13 months and 83
INTRODUCTIO N - Karl Von Baer - Coined the term cleavage. Swammerdom - First time seen cleavage. Prevost and Dumas - Reported cleavage of frog's egg. Spllanzani - Detail
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Coronal Disassembly - If the existing restoration with marginal defect: - The crown was sectioned through the bucco-occlusaly , Christensen crown remover elevate and remove
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