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Conformational Protection - Azotobacter
In conformational protection a Fe-S redox protein provides protection to the enzyme. The protein gets oxidised in the presence of oxygen and Mg2+ and then forms a reversible complex with nitrogenase enzyme. So the enzyme becomes inaccessible to oxygen and N2. In the absence of oxygen reduction of Fe-S redox protein results in the dissociation of the complex leading to recovery of active nitrogenase.
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supernatentmeans what
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