Composition - culture medium, Biology

Assignment Help:

Composition - Culture Medium

A standard plant tissue culture medium (Basal medium) contains all the essential macro elements (Carbon, hydrogen, oxygen nitrogen, phosphorus, sulphar, calcium, potassium and magnesium) and iron and microelements(iron, manganese, copper, zinc, boron and molybdenum) but the concentration of their salts in different formulations vary. In addition some vitamins and sucrose (2-3%) are universal constituents of plant tissue culture media. The composition of Ms medium (Murashige and Skoog's) medium found satisfactory for a wide range of monocotyledonous and dicotyledons species.

Besides the nutrients, one or more plant growth regulators (PGR7S) are generally required for supporting good growth of the cultured material. The PGR'S most widely used in tissue culture media are auxins (2, 4-D, IAA, NAA, IBA) and cytokinins (BAP, Kinetin). The PGR7s are particularly important for the growth of Callus tissues and organogenic embryogenic differentiation. Usually the medium is gelled with 0.8% bacteriological agar.


Related Discussions:- Composition - culture medium

What do all organic compounds contain, What do all organic compounds contai...

What do all organic compounds contain? The key element is carbon. Organic compounds are all carbon-having compounds. By definition, an organic compound having carbon. Alt

Define the ionizing radiation for sterilization, Q. Define the Ionizing rad...

Q. Define the Ionizing radiation for sterilization? Ionizing radiation are high energy electromagnetic waves including X-rays, gamma rays, particulate radiation, cosmic rays wh

Explain bidirectional glenn shunt under cardiopulmonary, Explain Bidirectio...

Explain Bidirectional Glenn Shunt under cardiopulmonary Bypass ? The ascending aorta and SVC at innominate jugular vein junction and IVC are cannulated and child connected to c

Explain germ layers that form tissues and organs in animals, Q. What are th...

Q. What are the three types of germ layers that form tissues and organs in animals? The three germ layers are the mesoderm, the ectoderm and the endoderm.

Explain the uses of hydrocolloids, Explain the uses of hydrocolloids In...

Explain the uses of hydrocolloids In particular, hydrocolloids can provide water for increasing the flexibility (plasticizing) of other food components. These can also affect i

Explain the ecosystems, Explain the Ecosystems? The ecosystem is yet an...

Explain the Ecosystems? The ecosystem is yet another level, or ecological unit, studied by ecologists. An ecosystem encompasses all of the organisms (all the populations-the en

Explain capillaries of the vascular system, Q. What are the capillaries of ...

Q. What are the capillaries of the vascular system? Capillaries are small blood vessels that perform exchange of substances between the tissues and blood the body Capillaries a

Describe ecological phytosociological, Q. Describe Ecological phytosociolog...

Q. Describe Ecological phytosociological? Field studies are desirable for an understanding of the relationship of any group of plants. If it is not possible to study the plants

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd