Coagulation - blood collection , Biology

Assignment Help:

Coagulation - Blood Collection:

Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies.

There are many types of anticoagulants used nowadays for example:

  • Heparin (salt of mucoitin polysulforic acid) is widely used and causes the least interference with the tests. About 20 units of heparin is needed to anticoagulate 1 ml of blood.
  • Ethylenediaminetetraacitic acid (EDTA): this chelating agent is presented as salt with final effective concentration of 1-2 mg/ml of blood. It should not be used for specimens tested for calcium analysis.
  • Sodium fluoride: this is generally considered as a preservative of glucose (it inhibit enzyme system involved in glycolysis); however it has weak anticoagulant activity. Fluoride at a concentration of about 2 mg/ml is the best preservative for glucose. Most specimens are preserved at 25 oC for 24 hr or 4 oC for 48 hr. Urea can not be estimated (in urease based methods) in a sample with fluoride because it inhibit the enzyme urease.
  • Other anticoagulants used are for example sodium citrate, oxalates, and iodoacetate.

          For estimation of bicarbonate in blood, it should be collected under one inch column of liquid paraffin to avoid exposure to the atmosphere, before it is centrifuged and processed. This is to avoid escape of carbon dioxide.

 

 


Related Discussions:- Coagulation - blood collection

What is prostaglandins , Prostaglandins and the structurally associated mol...

Prostaglandins and the structurally associated molecules prostacyclins, leukotrienes and thromboxanes, are called as eicosanoids because they having 20 carbon atoms. These hormones

Urine collection, Urine Collection: Untimed or random urine specimens ...

Urine Collection: Untimed or random urine specimens are suitable only for few chemical tests (mainly qualitative test). Usually urine sample is collected over a predetermined

Which does not contain a guanidinium group, Which of the following does NOT...

Which of the following does NOT contain a guanidinium group Select one: a. Urea b. arginine c. creatine d. guanidinium ion

Explain oxidation-reduction potential, Oxidation-reduction  Potential (ORP)...

Oxidation-reduction  Potential (ORP) ORP is related to the concentration of oxidizers or reducers in a solution, and their activity or strength. It provides an indication of th

Economic aspect of biodiversity, Q. Economic aspect of biodiversity? Bi...

Q. Economic aspect of biodiversity? Biodiversity serves as an income generating activity for countries around the world. Many people visit forests, beaches, mountains, grasslan

Role of carbohydrates in dyslipidemia, Q. Role of Carbohydrates in dyslipid...

Q. Role of Carbohydrates in dyslipidemia? It is important to know about these carbohydrates, as they all differ in their digestive properties. The rate of absorption is variabl

How many sodium dodecyl sulfate dissolve in 80 mls, How many grams of sodiu...

How many grams of sodium dodecyl sulfate (mw = 288.37) would you dissolve in 80 mLs of water to make a 20% solution of SDS?

What is exudative diarrhoea, Q. What is Exudative diarrhoea? Exudative ...

Q. What is Exudative diarrhoea? Exudative diarrhoea: It is associated with the mucosal damage leading to out pouring of mucus, blood and plasma proteins with a net accumulation

Endodontics principles and practice, Endodontics principles and practice ...

Endodontics principles and practice According to American association of endodontic, retreatment defined as:A procedure to remove root canal filling materials from the tooth vi

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd