Coagulation - blood collection , Biology

Assignment Help:

Coagulation - Blood Collection:

Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies.

There are many types of anticoagulants used nowadays for example:

  • Heparin (salt of mucoitin polysulforic acid) is widely used and causes the least interference with the tests. About 20 units of heparin is needed to anticoagulate 1 ml of blood.
  • Ethylenediaminetetraacitic acid (EDTA): this chelating agent is presented as salt with final effective concentration of 1-2 mg/ml of blood. It should not be used for specimens tested for calcium analysis.
  • Sodium fluoride: this is generally considered as a preservative of glucose (it inhibit enzyme system involved in glycolysis); however it has weak anticoagulant activity. Fluoride at a concentration of about 2 mg/ml is the best preservative for glucose. Most specimens are preserved at 25 oC for 24 hr or 4 oC for 48 hr. Urea can not be estimated (in urease based methods) in a sample with fluoride because it inhibit the enzyme urease.
  • Other anticoagulants used are for example sodium citrate, oxalates, and iodoacetate.

          For estimation of bicarbonate in blood, it should be collected under one inch column of liquid paraffin to avoid exposure to the atmosphere, before it is centrifuged and processed. This is to avoid escape of carbon dioxide.

 

 


Related Discussions:- Coagulation - blood collection

Determine the meaning of response cost, Determine the meaning of Response c...

Determine the meaning of Response cost This is a type of punishment in which the subject has to return back the token (positive reinforcement) earned earlier if the subject dis

Determine dominant individual fromhomozygous or heterozygous, Why can cross...

Why can crossing of an individual that manifests dominant phenotype with another that manifests recessive phenotype (for the same trait) determine whether the dominant individual i

Explain different photic energy frequencies, What is an example of a lab ex...

What is an example of a lab experiment that shows the variation of the photosynthesis efficiency in relation to different photic energy frequencies to which the reaction is exposed

Show metabolic rate of aerobic organisms, Q. Why can be the consumption of ...

Q. Why can be the consumption of molecular oxygen indicates the metabolic rate of aerobic organisms? Molecular oxygen (O 2 ) consumption has direct relation to the cell metabol

Could this be accomplished by passive diffusion, In the human body, the pot...

In the human body, the potassium ion can pass easily through cell membranes, yet the potassium ion concentration is higher inside many cells than it is outside these cells. Could t

True cyst- endodontics principles and practice, True cyst: cyst that is co...

True cyst: cyst that is completely lined by epithelial tissues.All the lesions are far away without communication with endodontic access or endodontic cavity or periapical

Why do protease-supplying cells of the stomach, Q Why do protease-supplying...

Q Why do protease-supplying cells of the stomach and of the pancreas make only precursors of the active proteolytic enzymes? The stomach and the pancreas make zymogens of the p

Protoplasm, Protoplasm Also known as bioplast . It is gelly like...

Protoplasm Also known as bioplast . It is gelly like & semi liquid in appearance. It is translucent in nature. It is colourless, oderless, viscous, elastic & granu

Planning of the nursing care, Planning of the Nursing Care 1) Educa...

Planning of the Nursing Care 1) Educate and teach the parents 2) Provide preoperative and postoperative care. Educate an Teach the Parents The parents may be

Cell wall, Cell wall is the structure produced by some of the cells outsid...

Cell wall is the structure produced by some of the cells outside their cell membrane; variously composed of the chitin, peptidoglycan, or cellulose.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd