Coagulation - blood collection , Biology

Assignment Help:

Coagulation - Blood Collection:

Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies.

There are many types of anticoagulants used nowadays for example:

  • Heparin (salt of mucoitin polysulforic acid) is widely used and causes the least interference with the tests. About 20 units of heparin is needed to anticoagulate 1 ml of blood.
  • Ethylenediaminetetraacitic acid (EDTA): this chelating agent is presented as salt with final effective concentration of 1-2 mg/ml of blood. It should not be used for specimens tested for calcium analysis.
  • Sodium fluoride: this is generally considered as a preservative of glucose (it inhibit enzyme system involved in glycolysis); however it has weak anticoagulant activity. Fluoride at a concentration of about 2 mg/ml is the best preservative for glucose. Most specimens are preserved at 25 oC for 24 hr or 4 oC for 48 hr. Urea can not be estimated (in urease based methods) in a sample with fluoride because it inhibit the enzyme urease.
  • Other anticoagulants used are for example sodium citrate, oxalates, and iodoacetate.

          For estimation of bicarbonate in blood, it should be collected under one inch column of liquid paraffin to avoid exposure to the atmosphere, before it is centrifuged and processed. This is to avoid escape of carbon dioxide.

 

 


Related Discussions:- Coagulation - blood collection

What is digital periapical radiography, Q. What is Digital periapical radio...

Q. What is Digital periapical radiography? Digital radiographs can be subjected to image processing with which the images can be altered to achieve task specific image characte

State the concept of finger tapping, State the concept of Finger Tapping ...

State the concept of Finger Tapping The subject is asked to tap his or her extended index finger on a typewriter key attached to a mechanical counter. Several series of 10-seco

Biting mechanism of snakes, i want to know the contents which i should writ...

i want to know the contents which i should write in my assignment..related to the above topic..

Where do pga and glycine gain entry, Where do PGA and glycine gain entry re...

Where do PGA and glycine gain entry respectively after being formed during photorespiration in plants? What occurs to them immediately after?

Fats , FATS ( = True Fats, Triglycerides, Neutral Fats) They are es...

FATS ( = True Fats, Triglycerides, Neutral Fats) They are esters of fatty acids and 3-carbon trihydric alcohol glycerol (= glycerine). T rue fat is also called trig

Explain about the renal and urogenital system, Explain about the Renal and ...

Explain about the Renal and Urogenital System? Various studies have shown a decrease in the creatinine and insulin clearance. Due to the changes in glomerular structure of the

What is a brain freeze, What is a brain freeze? What is commonly referr...

What is a brain freeze? What is commonly referred to as 'brain freeze' happens when you ingest something that is very cold too rapidly. When the cold touches your soft palate i

Back cross, BACK CROSS When F 1  individual (Tt) is crossed with one of...

BACK CROSS When F 1  individual (Tt) is crossed with one of the two parents, either tall (TT) or dwarf (tt), such a cross is called the back cross. There are two possibilities

Define homocysteinemia, Possible mechanisms of increased risk are that hype...

Possible mechanisms of increased risk are that hyperhomocystinemia may impair release of nitric oxide form endothelial cells, stimulate proferation of atherogenic smooth muscle cel

Phylum apicomplexa - protozoan, Phylum Apicomplexa - Protozoan Charact...

Phylum Apicomplexa - Protozoan Characteristic set of organelles (apical complex) asssociated with the anterior end present in some developmental stages. Cilia and flagella abs

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd