Coagulation - blood collection , Biology

Assignment Help:

Coagulation - Blood Collection:

Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies.

There are many types of anticoagulants used nowadays for example:

  • Heparin (salt of mucoitin polysulforic acid) is widely used and causes the least interference with the tests. About 20 units of heparin is needed to anticoagulate 1 ml of blood.
  • Ethylenediaminetetraacitic acid (EDTA): this chelating agent is presented as salt with final effective concentration of 1-2 mg/ml of blood. It should not be used for specimens tested for calcium analysis.
  • Sodium fluoride: this is generally considered as a preservative of glucose (it inhibit enzyme system involved in glycolysis); however it has weak anticoagulant activity. Fluoride at a concentration of about 2 mg/ml is the best preservative for glucose. Most specimens are preserved at 25 oC for 24 hr or 4 oC for 48 hr. Urea can not be estimated (in urease based methods) in a sample with fluoride because it inhibit the enzyme urease.
  • Other anticoagulants used are for example sodium citrate, oxalates, and iodoacetate.

          For estimation of bicarbonate in blood, it should be collected under one inch column of liquid paraffin to avoid exposure to the atmosphere, before it is centrifuged and processed. This is to avoid escape of carbon dioxide.

 

 


Related Discussions:- Coagulation - blood collection

Does the molecule will remain unchanged, A double-stranded piece of DNA, 10...

A double-stranded piece of DNA, 100 base pairs in size, is exposed to a high pH environment. This causes all non-valent interactions to be disrupted. What will result from this tre

Can you explain tannins, Q. Can you explain Tannins? Tannins are anoth...

Q. Can you explain Tannins? Tannins are another class of compounds which interfere with the absorption of minerals like iron and reduce the availability of proteins by bindin

Disease, what is the differnce between syndrome and symptom?

what is the differnce between syndrome and symptom?

What is wall resistance of plant cells, Q. What is wall resistance of plant...

Q. What is wall resistance of plant cells? Does this resistance make difficult the entrance of water into the cell or facilitate? Turgor Pressure TP, or Wall resistance, is the

Is a viral infection treated with drug, Q. Is a viral infection treated wit...

Q. Is a viral infection treated with the same kind of drug that treats bacterial infections? The Antibacterial drugs, potent against a great variety of bacteria, are not effect

Define factors affecting absorption of dietary iron, Define Factors affecti...

Define Factors affecting Absorption of Dietary Iron? There are mainly four factors that determine iron bioavailability absorption from the diet. These include: i) Form of ir

Porifera, general cherecter and anatomical stucture

general cherecter and anatomical stucture

Explain resistance to infection in nutritional care, Explain Resistance to ...

Explain Resistance to Infection in Nutritional Care? Amino acids help to build the body's defence mechanisms like antibodies, blood cells, hormones and enzymes so as to prevent

Physiology of respiration, PHYSIOLOG Y OF RESPIRATION - 1 .      EXC...

PHYSIOLOG Y OF RESPIRATION - 1 .      EXCHANGE OF GASES - It is Haemotasis. It takes place in Alveoli between alveolar air and arterial cappilary by diffusion i.e., f

Bacterial flagella structure and protocol, #qprokroyotic flagella struct...

#qprokroyotic flagella structure and protocoluestion..

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd