Coagulation - blood collection , Biology

Assignment Help:

Coagulation - Blood Collection:

Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies.

There are many types of anticoagulants used nowadays for example:

  • Heparin (salt of mucoitin polysulforic acid) is widely used and causes the least interference with the tests. About 20 units of heparin is needed to anticoagulate 1 ml of blood.
  • Ethylenediaminetetraacitic acid (EDTA): this chelating agent is presented as salt with final effective concentration of 1-2 mg/ml of blood. It should not be used for specimens tested for calcium analysis.
  • Sodium fluoride: this is generally considered as a preservative of glucose (it inhibit enzyme system involved in glycolysis); however it has weak anticoagulant activity. Fluoride at a concentration of about 2 mg/ml is the best preservative for glucose. Most specimens are preserved at 25 oC for 24 hr or 4 oC for 48 hr. Urea can not be estimated (in urease based methods) in a sample with fluoride because it inhibit the enzyme urease.
  • Other anticoagulants used are for example sodium citrate, oxalates, and iodoacetate.

          For estimation of bicarbonate in blood, it should be collected under one inch column of liquid paraffin to avoid exposure to the atmosphere, before it is centrifuged and processed. This is to avoid escape of carbon dioxide.

 

 


Related Discussions:- Coagulation - blood collection

Regulation of pyruvate kinase, Regulation of pyruvate kinase, pyruvate carb...

Regulation of pyruvate kinase, pyruvate carboxylase and PEP carboxykinase A) In liver,  pyruvate  kinase  is inhibited  by high levels  of ATP and alanine  so which  glycolysis

Define micronutrients requirements for anorexia nervosa, Define Micronutrie...

Define Micronutrients Requirements for Anorexia Nervosa? It is advisable to include one 100% RDA multivitamin tablet with minerals. The use of prophylactic thiamine supplements

Define the symptoms in bone marrow of pernicious anaemia, Define the sympto...

Define the symptoms in Bone marrow of pernicious anaemia? The nucleated red cells of the marrow are greatly increased.  The successive nucleated cell stages in erythropoiesis a

Define root-end resection apicoectomy, Define Root-End Resection Apicoectom...

Define Root-End Resection Apicoectomy a. Examine the root surface before cutting for: cracks, anatomical variations, and quality of orthograde obturation. b. Cut the root en

Define classification of weight imbalance, Define Classification of Weight ...

Define Classification of Weight Imbalance? Obesity is defined as a condition with accumulation of excess body fat. Do you think that a measure of how much fat a person has in i

Explain stavudine, Explain Stavudine It can be given either in initial ...

Explain Stavudine It can be given either in initial combination therapy or after failure of regimens containing other NRTIs. Cross-resistance with zidovudine is frequent, ho

Chordata, Structure of plasmodium

Structure of plasmodium

Biotechnology, Determine sequence weights for the sequences ACTA, ACTT, CGT...

Determine sequence weights for the sequences ACTA, ACTT, CGTT, and AGAT in problem 1 by using Thompson, Higgins, and Gibson method a) compute pairwise distances between sequences

The probability of an individual in the trihybrid, The probability of an in...

The probability of an individual in a trihybrid F2 generation showing the dominant phenotype for just two traits?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd