Coagulation - blood collection , Biology

Assignment Help:

Coagulation - Blood Collection:

Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies.

There are many types of anticoagulants used nowadays for example:

  • Heparin (salt of mucoitin polysulforic acid) is widely used and causes the least interference with the tests. About 20 units of heparin is needed to anticoagulate 1 ml of blood.
  • Ethylenediaminetetraacitic acid (EDTA): this chelating agent is presented as salt with final effective concentration of 1-2 mg/ml of blood. It should not be used for specimens tested for calcium analysis.
  • Sodium fluoride: this is generally considered as a preservative of glucose (it inhibit enzyme system involved in glycolysis); however it has weak anticoagulant activity. Fluoride at a concentration of about 2 mg/ml is the best preservative for glucose. Most specimens are preserved at 25 oC for 24 hr or 4 oC for 48 hr. Urea can not be estimated (in urease based methods) in a sample with fluoride because it inhibit the enzyme urease.
  • Other anticoagulants used are for example sodium citrate, oxalates, and iodoacetate.

          For estimation of bicarbonate in blood, it should be collected under one inch column of liquid paraffin to avoid exposure to the atmosphere, before it is centrifuged and processed. This is to avoid escape of carbon dioxide.

 

 


Related Discussions:- Coagulation - blood collection

Cleavage, what are the chemical changes takes place during cleavage

what are the chemical changes takes place during cleavage

Explain the balance of the ecosystem, Some people believe that being a vega...

Some people believe that being a vegan is the most responsible way to get nutrients while still maintaining the balance of the ecosystem. Explain this rationale, giving solid infor

Hormones secreted by the thyroid gland, Q. What are the hormones secreted b...

Q. What are the hormones secreted by the thyroid gland? What are their functions? The thyroid secretes the triiodothyronine (T3), hormones thyroxine (T4) and calcitonin. T3

Discuss about sarcoplasmic reticulum, A healthy skeletal muscle fiber is is...

A healthy skeletal muscle fiber is isolated and has no external forces on it.  It has normal intracellular levels of ATP and is bathed in physiological saline.  Which of the follow

What are the structures that form the external ear, Q. What are the structu...

Q. What are the structures that form the external ear? What is its function? The internal ear comprises the auricle or pinna and the auditory canal its function is to conduct t

Day-neutral plants, Day-Neutral Plants  Besides SDP and LDP, those pla...

Day-Neutral Plants  Besides SDP and LDP, those plants that flower irrespective of the length of light are called day-neutral plants. For these there is no specific requirement

Explain zalcitabine and adverse effects, Explain Zalcitabine and adverse ef...

Explain Zalcitabine and adverse effects Zalcitabine - Zalcitabine appears to be less effective, less convenient and more toxic than the other NRTIs; it is used rarely. Ad

Rationale for infection control, Q. Rationale for infection control? Th...

Q. Rationale for infection control? The answer lies in the fact that many infectious diseases may be transmitted during dental care. Source of potential infection that exists i

What is Implant Failure, What is Implant Failure Implant Failure : Th...

What is Implant Failure Implant Failure : The total failure of implant to fulfil its purpose which are49 Surgical Complications, Long Term Implant Failures and Ma

The Integumentary system, what are some technologys used in the integumenta...

what are some technologys used in the integumentaryy systems

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd