Coagulation - blood collection , Biology

Assignment Help:

Coagulation - Blood Collection:

Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies.

There are many types of anticoagulants used nowadays for example:

  • Heparin (salt of mucoitin polysulforic acid) is widely used and causes the least interference with the tests. About 20 units of heparin is needed to anticoagulate 1 ml of blood.
  • Ethylenediaminetetraacitic acid (EDTA): this chelating agent is presented as salt with final effective concentration of 1-2 mg/ml of blood. It should not be used for specimens tested for calcium analysis.
  • Sodium fluoride: this is generally considered as a preservative of glucose (it inhibit enzyme system involved in glycolysis); however it has weak anticoagulant activity. Fluoride at a concentration of about 2 mg/ml is the best preservative for glucose. Most specimens are preserved at 25 oC for 24 hr or 4 oC for 48 hr. Urea can not be estimated (in urease based methods) in a sample with fluoride because it inhibit the enzyme urease.
  • Other anticoagulants used are for example sodium citrate, oxalates, and iodoacetate.

          For estimation of bicarbonate in blood, it should be collected under one inch column of liquid paraffin to avoid exposure to the atmosphere, before it is centrifuged and processed. This is to avoid escape of carbon dioxide.

 

 


Related Discussions:- Coagulation - blood collection

Specific considerations and applied aspects in dentistry, Specific consider...

Specific considerations and applied aspects in dentistry The dental chair and delivery system. The chair should be smooth and seamless. The greatest potential for cross contami

What is molecular formula of the compound, A solid compound was found to co...

A solid compound was found to contain 42.11% C, 51.46%O and 6.43% H by weight. The molecular weight of the compound was 341. What is the molecular formula of the compound?

Advice on dischargeperi operative problems, Advice on Discharge :  Patient...

Advice on Discharge :  Patients are allowed to go home on 8th or 9th day. It could be even earlier after off pump coronary artery surgery (OPCAB). They should gradually increase t

What is more and less biologically complex, What is more and less biologica...

What is more and less biologically complex out of whey protien and ice cream and why?

Diseases caused by viruses, Diseases Caused by Viruses In the early day...

Diseases Caused by Viruses In the early days of microbiology, the disease producing submicroscopic agents were termed as 'filterable viruses' because of their ability to pass t

What is embryonic origin of the nervous system in vertebrate, How does the ...

How does the embryo turn from gastrula into neurula? How is the neural tube formed? What is the embryonic origin of the nervous system in vertebrates? The neurula stage is divi

How are parasites classified, Concerning the number of hosts how are parasi...

Concerning the number of hosts how are parasites classified? Parasites that need only one host are known as monoxenous parasites. Parasites that need more than one host for th

Define the recommended dietary allowance of vitamin e, Define the Recommend...

Define the Recommended Dietary Allowance of Vitamin E? The requirements for the vitamin E are expressed in terms of tocopherol equivalents (TE) as mentioned earlier - 8 mg fo

Energy yield of citric acid cycle , Each of the three NADH molecules formed...

Each of the three NADH molecules formed per turn of the cycle yields 3 ATPs and the one FADH2 yields 2 ATPs by oxidative phosphorylation (whereas some measurements indicate in whic

Can you define the vena cava, Q. What is the vena cava? Which type of blood...

Q. What is the vena cava? Which type of blood circulates within the vena cava? The vena cava is either of two large veins that debouch into the right atrium the superior vena c

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd