Coagulation - blood collection , Biology

Assignment Help:

Coagulation - Blood Collection:

Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies.

There are many types of anticoagulants used nowadays for example:

  • Heparin (salt of mucoitin polysulforic acid) is widely used and causes the least interference with the tests. About 20 units of heparin is needed to anticoagulate 1 ml of blood.
  • Ethylenediaminetetraacitic acid (EDTA): this chelating agent is presented as salt with final effective concentration of 1-2 mg/ml of blood. It should not be used for specimens tested for calcium analysis.
  • Sodium fluoride: this is generally considered as a preservative of glucose (it inhibit enzyme system involved in glycolysis); however it has weak anticoagulant activity. Fluoride at a concentration of about 2 mg/ml is the best preservative for glucose. Most specimens are preserved at 25 oC for 24 hr or 4 oC for 48 hr. Urea can not be estimated (in urease based methods) in a sample with fluoride because it inhibit the enzyme urease.
  • Other anticoagulants used are for example sodium citrate, oxalates, and iodoacetate.

          For estimation of bicarbonate in blood, it should be collected under one inch column of liquid paraffin to avoid exposure to the atmosphere, before it is centrifuged and processed. This is to avoid escape of carbon dioxide.

 

 


Related Discussions:- Coagulation - blood collection

Action of hormones, Action of Hormones We said earlier that hormones a...

Action of Hormones We said earlier that hormones are released into the blood stream or extracellular fluid and therefore, reach most of the cells of the body. However, they

What do you mean by polyester, Q. What do you mean by Polyester? Polyes...

Q. What do you mean by Polyester? Polyester (ES) or polyethylene terephthalate (PET): PET is a very strong transparent glossy film, which has good moisture and gas barrier p

Management of constipation, Q. Management of Constipation? You must hav...

Q. Management of Constipation? You must have realized by now that proper dietary and lifestyle management can help in maintaining the normal bowel movements to a great extent.

Define low - density lipoprotein, Q. Define Low - density lipoprotein? ...

Q. Define Low - density lipoprotein? Ans. LDL is the major cholesterol-rich lipoprotein carrying approximately 70 per cent of plasma cholesterol. It serves to transport ch

Excretory system, What is the excretory organ of an agama lizard?

What is the excretory organ of an agama lizard?

Planning and implementation of nursing care - purpura, Planning of Nursing ...

Planning of Nursing Care   Minimise  trauma  to the child and provide safe environment.  Control bleeding.  Administer drugs.  Implementation of Nursing Care

Is neuron b in the frog central nervous system, IS Neuron B in the frog cen...

IS Neuron B in the frog central nervous system Consider Neuron B in the frog central nervous system whose plasma membrane has a previously unknown channel that is selectly cond

Economically important fermentation products-beer, Normal 0 fal...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Congenital heart diseases, Congenital Heart Diseases   Congenital heart...

Congenital Heart Diseases   Congenital heart diseases are classified in many ways. One set of classification is cyanotic and acynotic conditions. Morphological classification

Explain about oxidation reduction potential, Q. Explain about Oxidation red...

Q. Explain about Oxidation reduction potential? The tendency of a substrate to accept or donate electrons is termed its redox potential (Eh). When a substrate loses electrons,

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd