Coagulation - blood collection , Biology

Assignment Help:

Coagulation - Blood Collection:

Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies.

There are many types of anticoagulants used nowadays for example:

  • Heparin (salt of mucoitin polysulforic acid) is widely used and causes the least interference with the tests. About 20 units of heparin is needed to anticoagulate 1 ml of blood.
  • Ethylenediaminetetraacitic acid (EDTA): this chelating agent is presented as salt with final effective concentration of 1-2 mg/ml of blood. It should not be used for specimens tested for calcium analysis.
  • Sodium fluoride: this is generally considered as a preservative of glucose (it inhibit enzyme system involved in glycolysis); however it has weak anticoagulant activity. Fluoride at a concentration of about 2 mg/ml is the best preservative for glucose. Most specimens are preserved at 25 oC for 24 hr or 4 oC for 48 hr. Urea can not be estimated (in urease based methods) in a sample with fluoride because it inhibit the enzyme urease.
  • Other anticoagulants used are for example sodium citrate, oxalates, and iodoacetate.

          For estimation of bicarbonate in blood, it should be collected under one inch column of liquid paraffin to avoid exposure to the atmosphere, before it is centrifuged and processed. This is to avoid escape of carbon dioxide.

 

 


Related Discussions:- Coagulation - blood collection

Class of subphylum uniramia - chilopoda, Class of Subphylum Uniramia - Chil...

Class of Subphylum Uniramia - Chilopoda Chilopoda have the centipedes. The class contains some 2500 species that have been described so far. They inhabit both of the tropical

Show the process of healing abutment, Q. Show the process of Healing abutme...

Q. Show the process of Healing abutment? Healing abutments are therefore not considered ideal. It is for this reason that the definitive abutment, selected at first stage surge

Match the dehydration reaction, Match dehydration reaction and hydrolysis r...

Match dehydration reaction and hydrolysis reaction to molecule synthesis and molecule break-down.

What is an endospore - staining strategies, What is an endospore? An en...

What is an endospore? An endospore is a specialized, highly resistant, dormant structure formed within the vegetative cell of some bacteria e.g. Bacillus (rod), Clostridium (ro

What is single cross between two mountain rabbits, In mountain rabbits, the...

In mountain rabbits, the EL-1 gene is located on chromosome 3. Seven alleles of this gene have been identified in the population. With respect to EL-1, what is the maximum number o

Define the term prebiotics, Define the term Prebiotics? Dietary Sources...

Define the term Prebiotics? Dietary Sources and their Mode of Action/health Effects We have already seen how prebiotics are defined. Let us go a little in-depth about them. Pre

Problem areas in integrative and environmental biology, Q Identify truly ex...

Q Identify truly exciting problem areas in integrative and environmental biology that need an integrated approach utilizing mathematics and statistics. What questions absolutely n

Which foods are used by microorganisms for energy, Q. Which Foods are used ...

Q. Which Foods are used by Microorganisms for Energy? The carbohydrates, especially the sugars, are most commonly used as an energy source, but other carbon compounds can also

What are the terms used in sterilization, Q. What are the terms used in ste...

Q. What are the terms used in sterilization? Sterilization: It is defined as freeing the object or substance from all life of any kind. It is defined alternatively as the

How are mammals characterized, Q. Mammal identity card. How are mammals cha...

Q. Mammal identity card. How are mammals characterized according to examples of representing beings, skin, respiration, nitrogen waste, basic morphology, circulation, thermal contr

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd