Coagulation - blood collection , Biology

Assignment Help:

Coagulation - Blood Collection:

Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies.

There are many types of anticoagulants used nowadays for example:

  • Heparin (salt of mucoitin polysulforic acid) is widely used and causes the least interference with the tests. About 20 units of heparin is needed to anticoagulate 1 ml of blood.
  • Ethylenediaminetetraacitic acid (EDTA): this chelating agent is presented as salt with final effective concentration of 1-2 mg/ml of blood. It should not be used for specimens tested for calcium analysis.
  • Sodium fluoride: this is generally considered as a preservative of glucose (it inhibit enzyme system involved in glycolysis); however it has weak anticoagulant activity. Fluoride at a concentration of about 2 mg/ml is the best preservative for glucose. Most specimens are preserved at 25 oC for 24 hr or 4 oC for 48 hr. Urea can not be estimated (in urease based methods) in a sample with fluoride because it inhibit the enzyme urease.
  • Other anticoagulants used are for example sodium citrate, oxalates, and iodoacetate.

          For estimation of bicarbonate in blood, it should be collected under one inch column of liquid paraffin to avoid exposure to the atmosphere, before it is centrifuged and processed. This is to avoid escape of carbon dioxide.

 

 


Related Discussions:- Coagulation - blood collection

Spoilage of pasteurized milk, Q. Explain Spoilage of pasteurized milk? ...

Q. Explain Spoilage of pasteurized milk? You would recall that pasteurization is one of the techniques to control and destroy the pathogenic, as well as, .spoilage bacteria i

How sugar is used in bakery application, How Sugar is used in Bakery Applic...

How Sugar is used in Bakery Application? In bakery applications, sugars are used to impart flavor, aroma and color. During the mixing process, excess gluten development can mak

A leaf is detached from a tree and tested with iodine, A leaf is detached f...

A leaf is detached from a tree and tested with iodine. The leaf turns dark blue.    (a) What does this result tell you?    (b) Why is this result not sufficient e

Determine the structural classification of bone, Structural classification ...

Structural classification of bone Macroscopically, bone tissue can be classified into two types, compact and trabecular bone. Compact bone is dense, corticated and makes up the

Explain the lectins or haemagglutinins, Explain the Lectins or Haemagglutin...

Explain the Lectins or Haemagglutinins? These are sugar binding proteins, having the ability to bind and agglutinate red blood cells (RBCs). You will find that they occur in mo

Landslides: measures to be taken during landslides, Protection of our prope...

Protection of our property:                  ·          If any our property lies in an area prone to landslides, we should seek advice of experts in earth movement and take cor

Loudness, The sound levels of power, intensity and pressure are the physica...

The sound levels of power, intensity and pressure are the physical effects which the human ear receives, it is not, however, what the person perceives. What is being perceived or h

Types of ribosomes, TYPE S OF RIBOSOMES Ribosomes are of two types: - ...

TYPE S OF RIBOSOMES Ribosomes are of two types: - 1 . Procaryotic Ribosomes - 70 s = Occur in cytoplasm of prokaryotes, and also in mitochondria, and Chloroplast o

Determine the uses of iron - soil, Determine the uses of Iron - Soil Ir...

Determine the uses of Iron - Soil Iron is a constituent of cytochromes, haem and non-haem enzymes. It is also essential for chlorophyll formation though is not a part of it. It

Polypeptide chains of hemoglobin, Q. In sickle cell anemia, a hereditary di...

Q. In sickle cell anemia, a hereditary disease, there is replacement of one amino acid by another in one of the four polypeptide chains of hemoglobin. In this case are all of the s

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd