Coagulation - blood collection , Biology

Assignment Help:

Coagulation - Blood Collection:

Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies.

There are many types of anticoagulants used nowadays for example:

  • Heparin (salt of mucoitin polysulforic acid) is widely used and causes the least interference with the tests. About 20 units of heparin is needed to anticoagulate 1 ml of blood.
  • Ethylenediaminetetraacitic acid (EDTA): this chelating agent is presented as salt with final effective concentration of 1-2 mg/ml of blood. It should not be used for specimens tested for calcium analysis.
  • Sodium fluoride: this is generally considered as a preservative of glucose (it inhibit enzyme system involved in glycolysis); however it has weak anticoagulant activity. Fluoride at a concentration of about 2 mg/ml is the best preservative for glucose. Most specimens are preserved at 25 oC for 24 hr or 4 oC for 48 hr. Urea can not be estimated (in urease based methods) in a sample with fluoride because it inhibit the enzyme urease.
  • Other anticoagulants used are for example sodium citrate, oxalates, and iodoacetate.

          For estimation of bicarbonate in blood, it should be collected under one inch column of liquid paraffin to avoid exposure to the atmosphere, before it is centrifuged and processed. This is to avoid escape of carbon dioxide.

 

 


Related Discussions:- Coagulation - blood collection

Phylum labyrinthomorpha - protozoan, Phylum Labyrinthomorpha - Protozoan ...

Phylum Labyrinthomorpha - Protozoan Trophic stage, ectoplasmic network with spindle shaped or spherical nonamoeboid cells. Small group living on algae; mostly marine or estuar

Zoonoses disease- histoplamosis, Histoplamosis Caveran disease, darling’s ...

Histoplamosis Caveran disease, darling’s disease, reticuloendothelial cytomycosis or tingo maria fever are the other synonyms for histoplamosis caused by Histoplasma capsulatum. T

Rna processing , In the prokaryotes,  RNA transcribed  from pr...

In the prokaryotes,  RNA transcribed  from protein-coding  genes or messenger  RNA, mRNA,  need  little  or  no  changes  prior  to  translation.  In reality, several mRNA   molecu

Explain the mechanisms of heat loss, Name and explain the mechanisms of hea...

Name and explain the mechanisms of heat loss that are contributing to Joel's feelings of coldness. Be sure to name ALL the mechanisms that are contributing!

Explain esterification, Explain Esterification The process of convertin...

Explain Esterification The process of converting an acid into an alkyl or aryl derivative. Most frequently the process consists of the reaction of an acid with an alcohol in th

Determine the biologic response, Determine the biologic response The bi...

Determine the biologic response The biologic response can include: i) Metabolic disturbance. ii) Inflammatory response iii) Immune response iv) Mutagenesis v) Ca

What is the average duration of each stage in hours, A biologist examines a...

A biologist examines a series of cells and counts 140 cells in interphase, 10 cells in metaphase, 4 cells in anaphase and 7 cells in telophase. if complete cell cycle requires 24 h

Determine effective half life and value of effective decay, 1. By direct me...

1. By direct measurement of the activity of the thyroid gland in several patients who had received 131 I for diagnostic purposes, it was observed that the biological elimination h

Diagnosis, The symptoms and signs of endocarditis are often constitutional ...

The symptoms and signs of endocarditis are often constitutional and, when localized, often result from a complication of IE rather than reflect the intracardiac infection itself. C

Animal classification, Explain metachrosis in frogs? How it is different fr...

Explain metachrosis in frogs? How it is different from change of skin colour of chemleon?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd