Coagulation - blood collection , Biology

Assignment Help:

Coagulation - Blood Collection:

Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies.

There are many types of anticoagulants used nowadays for example:

  • Heparin (salt of mucoitin polysulforic acid) is widely used and causes the least interference with the tests. About 20 units of heparin is needed to anticoagulate 1 ml of blood.
  • Ethylenediaminetetraacitic acid (EDTA): this chelating agent is presented as salt with final effective concentration of 1-2 mg/ml of blood. It should not be used for specimens tested for calcium analysis.
  • Sodium fluoride: this is generally considered as a preservative of glucose (it inhibit enzyme system involved in glycolysis); however it has weak anticoagulant activity. Fluoride at a concentration of about 2 mg/ml is the best preservative for glucose. Most specimens are preserved at 25 oC for 24 hr or 4 oC for 48 hr. Urea can not be estimated (in urease based methods) in a sample with fluoride because it inhibit the enzyme urease.
  • Other anticoagulants used are for example sodium citrate, oxalates, and iodoacetate.

          For estimation of bicarbonate in blood, it should be collected under one inch column of liquid paraffin to avoid exposure to the atmosphere, before it is centrifuged and processed. This is to avoid escape of carbon dioxide.

 

 


Related Discussions:- Coagulation - blood collection

What is deletion of cell, What is Deletion of cell? A deletion is simpl...

What is Deletion of cell? A deletion is simply when a tip or fragment of a chromosome breaks off and fails to reattach itself to the chromosome from which it came. The gene, or

Similarities between pisces and amphibians, Ask question #Minimuwhat are th...

Ask question #Minimuwhat are the similarities between pisces and amphibians

Draw a graph that concerns a biological subject, Graphs are a way to convey...

Graphs are a way to convey information and trends in a convenient fashion. For this week's Discussion Board you should search the internet for a graph related to biology. Provide u

Show appearance of food product, Q. Show Appearance of food product? Ap...

Q. Show Appearance of food product? Appearance can be judged by the eye. Appearance factors include such things as size, colour, uniformity, absence of any defect, shape, whole

Reproduction, Normal 0 false false false EN-IN X-NONE...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Mechanisms of sex determination and dosage compensation, XYY humans are fer...

XYY humans are fertile males. XXX humans are fertile females. What do these observations reveal about the mechanisms of sex determination and dosage compensation?

Sphingolipids, What are Sphingolipids? Also there link to cancer caused in ...

What are Sphingolipids? Also there link to cancer caused in cells.

Explain about the deuteromycota - fungi, Explain about the Deuteromycota - ...

Explain about the Deuteromycota - Fungi? Deuteromycota - Deuteromycetes or fungi imperfecti reproduce by means of conidia. These have either lost the capacity for sexual reprod

Alcohol related emergencies, Alcohol Related Emergencies: Alcohol is o...

Alcohol Related Emergencies: Alcohol is one of  the most serious health problems. Alcoholism is defined as a primary or chronic disease with genetic, psychological and environ

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd