Coagulation - blood collection , Biology

Assignment Help:

Coagulation - Blood Collection:

Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies.

There are many types of anticoagulants used nowadays for example:

  • Heparin (salt of mucoitin polysulforic acid) is widely used and causes the least interference with the tests. About 20 units of heparin is needed to anticoagulate 1 ml of blood.
  • Ethylenediaminetetraacitic acid (EDTA): this chelating agent is presented as salt with final effective concentration of 1-2 mg/ml of blood. It should not be used for specimens tested for calcium analysis.
  • Sodium fluoride: this is generally considered as a preservative of glucose (it inhibit enzyme system involved in glycolysis); however it has weak anticoagulant activity. Fluoride at a concentration of about 2 mg/ml is the best preservative for glucose. Most specimens are preserved at 25 oC for 24 hr or 4 oC for 48 hr. Urea can not be estimated (in urease based methods) in a sample with fluoride because it inhibit the enzyme urease.
  • Other anticoagulants used are for example sodium citrate, oxalates, and iodoacetate.

          For estimation of bicarbonate in blood, it should be collected under one inch column of liquid paraffin to avoid exposure to the atmosphere, before it is centrifuged and processed. This is to avoid escape of carbon dioxide.

 

 


Related Discussions:- Coagulation - blood collection

Which responds to electrical signals, You have identified a protein in a ce...

You have identified a protein in a cell that you know that comes from the human body. With respect to that protein you have also determined that it allows sodium ions to go through

What is the kind of nitrogen waste that mammals eliminate, Q What is the ki...

Q What is the kind of nitrogen waste that mammals eliminate? Like chondrichtian fishes and adult amphibians, mammals are ureotelic that is they excrete urea. Q. How do plac

Pulp tissue revascularization, Pulp Tissue Revascularization :   Defi...

Pulp Tissue Revascularization :   Definition: Is the procedure to re-establish the vitality in non-vital tooth by allowing repair & regeneration of the tissues.

Determine about the soil and its importance, Determine about the Soil and i...

Determine about the Soil and its importance Everyone knows about soil as a resource which fulfils the basic requirement of human kind by supporting variety of plants and other

Illustrate about the primary minerals, Illustrate about the primary mineral...

Illustrate about the primary minerals The minerals originally formed when once -heated magma (molten rock) cooled and formed solid igneous rocks (explained below) are called as

Chemical weathering-formation of soil, Chemical Weathering The rocks wh...

Chemical Weathering The rocks while getting disintegrated may also undergo chemical change. Water is an important agent in bringing about chemical changes due to dissolution or

Types of cell respiration, Q What are the kinds of cell respiration? Th...

Q What are the kinds of cell respiration? There are two types of cell respiration aerobic cell respiration a reaction with participation of molecular oxygen (O2) and anaerobic

Do fishes there an open or closed circulatory system, Do fishes there an op...

Do fishes there an open or closed circulatory system? How many chambers does a fish heart have? How does blood flow all through the fish body? As in every vertebrate the circul

Explain the behavioural observations of the child, Explain the Behavioural ...

Explain the Behavioural Observations of the child Behavioural observations of the child are the second critical source of information available to the neuropsychologist. Qualit

Explain advantages of using yeast as a source of protein, Organism:- Yeast ...

Organism:- Yeast Advantages Large size, hence separation from the culture medium is easy.  As the pH of the growth is towards acidic side, high amount of lysine is prod

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd