Coagulation - blood collection , Biology

Assignment Help:

Coagulation - Blood Collection:

Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies.

There are many types of anticoagulants used nowadays for example:

  • Heparin (salt of mucoitin polysulforic acid) is widely used and causes the least interference with the tests. About 20 units of heparin is needed to anticoagulate 1 ml of blood.
  • Ethylenediaminetetraacitic acid (EDTA): this chelating agent is presented as salt with final effective concentration of 1-2 mg/ml of blood. It should not be used for specimens tested for calcium analysis.
  • Sodium fluoride: this is generally considered as a preservative of glucose (it inhibit enzyme system involved in glycolysis); however it has weak anticoagulant activity. Fluoride at a concentration of about 2 mg/ml is the best preservative for glucose. Most specimens are preserved at 25 oC for 24 hr or 4 oC for 48 hr. Urea can not be estimated (in urease based methods) in a sample with fluoride because it inhibit the enzyme urease.
  • Other anticoagulants used are for example sodium citrate, oxalates, and iodoacetate.

          For estimation of bicarbonate in blood, it should be collected under one inch column of liquid paraffin to avoid exposure to the atmosphere, before it is centrifuged and processed. This is to avoid escape of carbon dioxide.

 

 


Related Discussions:- Coagulation - blood collection

Differences between continuous traits and discrete traits, Can you please d...

Can you please describe the differences between continuous traits and discrete traits and how it helps an ecologist measure variation? Please give a few examples.

Major objectives of diarrhoea, Q. Major objectives of diarrhoea? The ma...

Q. Major objectives of diarrhoea? The major objectives in the management of diarrhoea include: 1. Fluid and electrolyte replacement 2. Removal of cause (especially if inf

Are environmental phenotypical changes transmitted offspring, Are environme...

Are environmental phenotypical changes transmitted to the offspring? Changes caused on the phenotypes by the environment are not transmitted to the offspring unless their prima

radioactive pollution, Radioactive pollution is a very special form of pol...

Radioactive pollution is a very special form of pollution of air, water and soil with radioactive material. Its nature of contamination and effects are different from those of othe

What is a population, Q. What is a population? In the Biology a populat...

Q. What is a population? In the Biology a population is a set of individuals of the same species living in a given place and in a given time.

Diabetes mellitus, Diabetes mellitus, Types I and II is a disorder regard...

Diabetes mellitus, Types I and II is a disorder regarding the defects in insulin action. Type I diabetes is characterized by an inadequate insulin secretion; Type II diabetes is

Is diabetes causes the cardiovascular disease, Q. Is Diabetes causes the ca...

Q. Is Diabetes causes the cardiovascular disease? Diabetes: Sustained hyperglycemia is associated with tissue damage and cardio- myopathies. You shall study about diabetes and

Explain resistance to infection in nutritional care, Explain Resistance to ...

Explain Resistance to Infection in Nutritional Care? Amino acids help to build the body's defence mechanisms like antibodies, blood cells, hormones and enzymes so as to prevent

Hyperthyroidism (graves disease), Hyperthyroidism (Graves Disease): Gr...

Hyperthyroidism (Graves Disease): Graves diseases is  the most common cause of hyperthyroidism  in  children and is usually associated with an  enlarged thyroid gland and exop

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd