Coagulation - blood collection , Biology

Assignment Help:

Coagulation - Blood Collection:

Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies.

There are many types of anticoagulants used nowadays for example:

  • Heparin (salt of mucoitin polysulforic acid) is widely used and causes the least interference with the tests. About 20 units of heparin is needed to anticoagulate 1 ml of blood.
  • Ethylenediaminetetraacitic acid (EDTA): this chelating agent is presented as salt with final effective concentration of 1-2 mg/ml of blood. It should not be used for specimens tested for calcium analysis.
  • Sodium fluoride: this is generally considered as a preservative of glucose (it inhibit enzyme system involved in glycolysis); however it has weak anticoagulant activity. Fluoride at a concentration of about 2 mg/ml is the best preservative for glucose. Most specimens are preserved at 25 oC for 24 hr or 4 oC for 48 hr. Urea can not be estimated (in urease based methods) in a sample with fluoride because it inhibit the enzyme urease.
  • Other anticoagulants used are for example sodium citrate, oxalates, and iodoacetate.

          For estimation of bicarbonate in blood, it should be collected under one inch column of liquid paraffin to avoid exposure to the atmosphere, before it is centrifuged and processed. This is to avoid escape of carbon dioxide.

 

 


Related Discussions:- Coagulation - blood collection

Contractile vacuoles - excretion, Normal 0 false false fals...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Discuss about electric field, Discuss about electric field The electric...

Discuss about electric field The electric field is a vector quantity whose magnitude is the force per unit charge and points in the direction of the force on the positive test

Explain the absorption, Explain the Absorption, Storage and Elimination of ...

Explain the Absorption, Storage and Elimination of Folate? Folic acid is readily absorbed from the small intestines through the portal vein and  passed onto the tissues throug

Is all the cartilage in the body the same, Q. Is all the cartilage in the b...

Q. Is all the cartilage in the body the same? There are 3 types of cartilage in the human body: 1) hyaline cartilage; 2) elastic cartilage and 3) fibrocartilage. Hyaline cartil

Viruses, are viruses cellular organisms

are viruses cellular organisms

What do you understand by arthropodization, What do you understand by Arthr...

What do you understand by Arthropodization? Many of the traits that we consider unique to the Arthropoda may not be independent traits. Instead, they may be the consequence of

Management with insulin, Type 1 Diabetes Mellitus occurs when the body's im...

Type 1 Diabetes Mellitus occurs when the body's immune system destroys beta cells. Beta cells produce insulin, a hormone that helps to move the glucose contained in food into cells

How fats promote absorption of fat soluble vitamins, 1. Fats promote absorp...

1. Fats promote absorption of fat soluble vitamins like vitamin A, D, E and K. Patients of cystic fibrosis often absorb fat poorly and are at-risk for fat-soluble vitamin deficienc

What are the major features of the meristematic cells, What are the major f...

What are the major features of the meristematic cells? And why do these cells need to have a high mitotic rate? The Meristematic cells have very thin cell walls, a well-central

Iron age, Explain the factors that led to the decline of Science in Europe ...

Explain the factors that led to the decline of Science in Europe during iron age

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd