Coagulation - blood collection , Biology

Assignment Help:

Coagulation - Blood Collection:

Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies.

There are many types of anticoagulants used nowadays for example:

  • Heparin (salt of mucoitin polysulforic acid) is widely used and causes the least interference with the tests. About 20 units of heparin is needed to anticoagulate 1 ml of blood.
  • Ethylenediaminetetraacitic acid (EDTA): this chelating agent is presented as salt with final effective concentration of 1-2 mg/ml of blood. It should not be used for specimens tested for calcium analysis.
  • Sodium fluoride: this is generally considered as a preservative of glucose (it inhibit enzyme system involved in glycolysis); however it has weak anticoagulant activity. Fluoride at a concentration of about 2 mg/ml is the best preservative for glucose. Most specimens are preserved at 25 oC for 24 hr or 4 oC for 48 hr. Urea can not be estimated (in urease based methods) in a sample with fluoride because it inhibit the enzyme urease.
  • Other anticoagulants used are for example sodium citrate, oxalates, and iodoacetate.

          For estimation of bicarbonate in blood, it should be collected under one inch column of liquid paraffin to avoid exposure to the atmosphere, before it is centrifuged and processed. This is to avoid escape of carbon dioxide.

 

 


Related Discussions:- Coagulation - blood collection

Determine causes of malnutrition, Determine Causes of Malnutrition? You...

Determine Causes of Malnutrition? You may recall studying about the causes of malnutrition earlier. The causes of malnutrition are classified as immediate (individual level), u

Manifestations of hypertension, Manifestations of Hypertension • Renal ...

Manifestations of Hypertension • Renal Failure • Left Ventricular Failure • Myocardial Infarction • Cerebral Haemorrhage

Explain pure culture techniques, Explain Pure Culture Techniques We lea...

Explain Pure Culture Techniques We learnt the techniques involved in sub-culturing, i.e., the process involved in transfer of culture from one medium to another or transfer of

What is ancylostomiasis, What is ancylostomiasis? Ancylostomiasis is a ...

What is ancylostomiasis? Ancylostomiasis is a disease caused by Ancylostoma duodenale or Necator americanus, both hookworms belonging to the nematode phylum (roundworms). Ancyl

Define criteria for assessment of riboflavin status, Define Criteria for As...

Define Criteria for Assessment of Riboflavin Status? Riboflavin status can be assessed by measuring urinary excretion of the vitamin in fasting, random, and 24-hour specimens o

Differences between anastral and astral mitosis, Q. What are the difference...

Q. What are the differences between anastral and astral mitosis? Astral mitosis is that in which there is formation of the aster a structure made by the centrioles. Anastral mi

Bioinformatics is important, Why do you think that bioinformatics is import...

Why do you think that bioinformatics is important?         Ans) Today, we can use computers to access much more biological data than still before. You can learn a lot by analysi

What is represented by the cellular secretion, Q. What is represented by th...

Q. What is represented by the cellular secretion? Cell secretion is the removal to the exterior of substances produced by the cell for instance, hormones, mucus, and sweat, so

Regulation of fatty acid, The main point of control of β-oxidation is the a...

The main point of control of β-oxidation is the availability of fatty acids.  The major  source   of  free  fatty   acids   in  the  blood   is  from   the  breakdown   of triacylg

Define visible hazards in food, Normal 0 false false false ...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd