Coagulation - blood collection , Biology

Assignment Help:

Coagulation - Blood Collection:

Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies.

There are many types of anticoagulants used nowadays for example:

  • Heparin (salt of mucoitin polysulforic acid) is widely used and causes the least interference with the tests. About 20 units of heparin is needed to anticoagulate 1 ml of blood.
  • Ethylenediaminetetraacitic acid (EDTA): this chelating agent is presented as salt with final effective concentration of 1-2 mg/ml of blood. It should not be used for specimens tested for calcium analysis.
  • Sodium fluoride: this is generally considered as a preservative of glucose (it inhibit enzyme system involved in glycolysis); however it has weak anticoagulant activity. Fluoride at a concentration of about 2 mg/ml is the best preservative for glucose. Most specimens are preserved at 25 oC for 24 hr or 4 oC for 48 hr. Urea can not be estimated (in urease based methods) in a sample with fluoride because it inhibit the enzyme urease.
  • Other anticoagulants used are for example sodium citrate, oxalates, and iodoacetate.

          For estimation of bicarbonate in blood, it should be collected under one inch column of liquid paraffin to avoid exposure to the atmosphere, before it is centrifuged and processed. This is to avoid escape of carbon dioxide.

 

 


Related Discussions:- Coagulation - blood collection

What is tagma, What is Tagma, Tagmosis and Tagmatization? Distinct body...

What is Tagma, Tagmosis and Tagmatization? Distinct body regions resulting when different segments of a metameric animal become involved in specific functions. These segments a

Classification, What is omnispective classification

What is omnispective classification

Difference between diffusion and active transport, What do facilitated diff...

What do facilitated diffusion and active transport have in common? What are the differences between them? Facilitated diffusion can be confused with active transport because in

Haversian canals and the volkmann canals of the bones, Q. What are the Have...

Q. What are the Haversian canals and the Volkmann's canals of the bones? Is the osseous tissue vascularized? The Haversian canals are longitudinal canals present in the osseous

Describe the various steps involved in glycolysis, Question 1 Give a detai...

Question 1 Give a detailed account on "high energy compounds" Question 2 Describe the various steps involved in Glycolysis Question 3 Give a detailed account on Oxidativ

Fate map - gastrulation in chick, Fate Map - Gastrulation in Chick All...

Fate Map - Gastrulation in Chick All the three main germinal layers (ectoderm, mesoderm, endoderm) are made from cells initially located in the epiblast in area pellucida, hen

Renal insufficiency, CHF complicating IE is primarily the result of valve d...

CHF complicating IE is primarily the result of valve destruction or distortion or rupture of chordae tendinae. Intracardiac fistulas, myocarditis, or coronary artery embolization m

Personal protective equipment, Personal Protective Equipment These eq...

Personal Protective Equipment These equipments are essentially required during working to protect face, eyes, respiratory system hands and clothes. Additionally in various in

How to investigate mitral stenosis by electrocardiogram, Q. How to Investig...

Q. How to Investigate mitral stenosis by Electrocardiogram? Electrocardiographic changes are not specific for mitral stenosis and are never diagnostic for any valvular heart di

What do you mean by digestive enzymes, Q. Besides the pancreatic juice in t...

Q. Besides the pancreatic juice in the intestine there is the releasing of the enteric juice that contains digestive enzymes too. What are these enzymes and which type of molecule

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd