Coagulation - blood collection , Biology

Assignment Help:

Coagulation - Blood Collection:

Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies.

There are many types of anticoagulants used nowadays for example:

  • Heparin (salt of mucoitin polysulforic acid) is widely used and causes the least interference with the tests. About 20 units of heparin is needed to anticoagulate 1 ml of blood.
  • Ethylenediaminetetraacitic acid (EDTA): this chelating agent is presented as salt with final effective concentration of 1-2 mg/ml of blood. It should not be used for specimens tested for calcium analysis.
  • Sodium fluoride: this is generally considered as a preservative of glucose (it inhibit enzyme system involved in glycolysis); however it has weak anticoagulant activity. Fluoride at a concentration of about 2 mg/ml is the best preservative for glucose. Most specimens are preserved at 25 oC for 24 hr or 4 oC for 48 hr. Urea can not be estimated (in urease based methods) in a sample with fluoride because it inhibit the enzyme urease.
  • Other anticoagulants used are for example sodium citrate, oxalates, and iodoacetate.

          For estimation of bicarbonate in blood, it should be collected under one inch column of liquid paraffin to avoid exposure to the atmosphere, before it is centrifuged and processed. This is to avoid escape of carbon dioxide.

 

 


Related Discussions:- Coagulation - blood collection

What are the factors to an actual shift in the demand curve, What are the f...

What are the factors to an actual shift in the demand curve? Factors that cause an actual shift within the demand curve are as follows: When prices rise or fall that would c

Define limitations for underwater weighing method, Define Limitations for u...

Define Limitations for underwater weighing method? This method is expensive, time consuming and usually requires a lot of equipment and space. The subject needs to be

Epididymis, EPIDIDYMIS - All seminiferous tubules form short straigh...

EPIDIDYMIS - All seminiferous tubules form short straight tubules as tubuli recti, open into wider network of rete testis. From rete testis 15-20 fine convoluted tubules,

Define flow phase in physiological response to injury, Define Flow Phase in...

Define Flow Phase in Physiological Response to injury? It is characterized by hyper metabolism, increased cardiac output, increased urinary nitrogen losses, altered glucose met

Explain the plating method - pure culture techniques, Explain the Plating M...

Explain the Plating Method - Pure Culture Techniques? Plating method is one of the commonly employed methods for getting a pure culture.  The procedure involves the separation

The graph as temperature increases differ, Does the trend of the change in ...

Does the trend of the change in shape of the graph as temperature increases differ when a different gas is examined?

Hydralazine, Hydralazine is a potent arteriolar dilator and markedly increa...

Hydralazine is a potent arteriolar dilator and markedly increases cardiac output in patients with congestive heart failure. However, as a single agent, it has not been shown to imp

Explain the minimum requirement of nutritional needs, Explain the Minimum R...

Explain the Minimum Requirement of nutritional needs? Minimum nutrient requirement is defined as the 'lowest amount of the nutrient ji-om the diet that will prevent clinically

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd