Coagulation - blood collection , Biology

Assignment Help:

Coagulation - Blood Collection:

Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies.

There are many types of anticoagulants used nowadays for example:

  • Heparin (salt of mucoitin polysulforic acid) is widely used and causes the least interference with the tests. About 20 units of heparin is needed to anticoagulate 1 ml of blood.
  • Ethylenediaminetetraacitic acid (EDTA): this chelating agent is presented as salt with final effective concentration of 1-2 mg/ml of blood. It should not be used for specimens tested for calcium analysis.
  • Sodium fluoride: this is generally considered as a preservative of glucose (it inhibit enzyme system involved in glycolysis); however it has weak anticoagulant activity. Fluoride at a concentration of about 2 mg/ml is the best preservative for glucose. Most specimens are preserved at 25 oC for 24 hr or 4 oC for 48 hr. Urea can not be estimated (in urease based methods) in a sample with fluoride because it inhibit the enzyme urease.
  • Other anticoagulants used are for example sodium citrate, oxalates, and iodoacetate.

          For estimation of bicarbonate in blood, it should be collected under one inch column of liquid paraffin to avoid exposure to the atmosphere, before it is centrifuged and processed. This is to avoid escape of carbon dioxide.

 

 


Related Discussions:- Coagulation - blood collection

What is spectroscopy, Question Write a short note on the following ...

Question Write a short note on the following 1 Simple diffusion 2 Viscosity 3 What is spectroscopy? List and explain types of spectroscopy 4 With a neat diagram,

Tropical seasonal forests - ecosystem, Tropical seasonal forests - Ecosyste...

Tropical seasonal forests - Ecosystem Tropical seasonal forests occur in regions where total annual rainfall is very high but segregated into pronounced wet and dry periods. T

Phylum, classification of animal vorticella

classification of animal vorticella

Define direct visualisation of coronary arteries, Q. Define Direct Visualis...

Q. Define Direct Visualisation of Coronary Arteries? Ans. Coronary arteries cad be directly visualized using two-dimensional echocardiography, especially in patients with

Define about the centrifuge, Define about the Centrifuge? Centrifuge is...

Define about the Centrifuge? Centrifuge is an apparatus consisting essentially of a compartment spun about a central axis to separate contained materials of different specific

Describe the incorporated protectants rules, Which actions proposed in 1994...

Which actions proposed in 1994 were not finalized in the recently issued plant- incorporated protectants rules ? EPA has shown in a supplemental notice, which is part of the fi

Baby delivery, some deliveries of baby occur early na. y is it so?

some deliveries of baby occur early na. y is it so?

Explain dextrins, Dextrins Dextrins are also polymers of D-glucose held...

Dextrins Dextrins are also polymers of D-glucose held by a(l,4) glycosidic linkages. Dextrins are,  in  fact,  formed due to partial hydrolysis of starch by  enzymes such as sa

Neurons, hpw many neurons do we have?

hpw many neurons do we have?

Determine the number of gametes, An organism has 16 chromosomes in its soma...

An organism has 16 chromosomes in its somatic cells. Determine the number of gametes with different chromosome compositions that it can potentially create. Explain your answer. Ign

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd