cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Standard invert sugar solution, Q. Standard invert sugar solution? Rea...

Q. Standard invert sugar solution? Reagents Required 1) Standard invert sugar solution: Weigh accurately 0.985 g of sucrose and dissolve in 500 ml of water. Add 2 ml of

What is sodium bicarbonate, What is Sodium Bicarbonate? The administrat...

What is Sodium Bicarbonate? The administration of bicarbonate may be considered if the pH remains below 7.1 or if hyperkalemia or tricyclic antidepressant overdose is suspected

Nuclear transfer method for gene transfer, Nuclear transfer method: Nucle...

Nuclear transfer method: Nuclear transfer will extend the range of species in which gene targeting will be possible and thereby provide better models to test treatments for human

How to load glycogen, How to load glycogen? Over the past fifty years, ...

How to load glycogen? Over the past fifty years, the biggest breakthrough was the discovery of how to load glycogen and sophistication in the methods of glycogen loading. Nitro

Fertilizers and fertilizer management, Fertilizers and Fertilizer Managemen...

Fertilizers and Fertilizer Management Fertilizers, in a broad sense, include all materials that are added to soils to supply certain elements essential to the growth of plants

Mathematical and statistical challenges, Progress on these biological issue...

Progress on these biological issues needs both that more mathematics and statistics be brought to bear on them and that new mathematical and statistical tools be prepared. All the

What do you mean by the good and bad cholesterol, Q. What are the so-called...

Q. What are the so-called "good" and "bad" cholesterol? Lipoproteins are complexes made of lipids (cholesterol and triglycerides) and proteins. The lipoproteins present differe

Determine the importance of educational history- assessment, Determine the ...

Determine the importance of Educational History- assessment  A complete school history includes information regarding a child's current grade placement, any grade repetitions o

Four characteristics of an efficient respiratory surface, State four charac...

State four characteristics of an efficient respiratory surface. Four characteristics of an well-organized respiratory surface are: a) thin epithelium, b) large surface ar

What is the endoplasmic reticulum, What is the Endoplasmic Reticulum Th...

What is the Endoplasmic Reticulum The cytoplasm of most eukaryotic cells contains a very complex network of internal membranes, called the endoplasmic reticulum, which forms ch

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd