cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Define mushroom poisoning, Q. Define Mushroom Poisoning? Mushroom poiso...

Q. Define Mushroom Poisoning? Mushroom poisoning is caused by the consumption of raw or cooked fruiting bodies (mushrooms, toadstools) of a number of species of higher fungl.

The dna revolution, Unprecedental clarity has come to our understanding of ...

Unprecedental clarity has come to our understanding of genetic differences by the analysis of DNA sequences. It is not surprising in which the latest DNA technologies are leading t

Destinations of the organic material fabricate by producers, Q. What are th...

Q. What are the destinations of the organic material fabricated by the producers? The Part of the organic material synthesized by the producers is consumed as energy source for

Indifferences in ionic composition, Indifferences in ionic composition Answ...

Indifferences in ionic composition Answer A. across membranes can be created through the action of ATP driven pumps as long as number of negative and positive ions on remain equal

Explain the superficial minor burns, Explain the Superficial Minor Burns? ...

Explain the Superficial Minor Burns? Superficial minor bums are immersed immediately in cool water if possible. The burn is carefully cleaned to prevent infection. If dirt is d

Adrenocortical steroids - vertebrates, Normal 0 false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Determine the statement- the evolution of color vision, Based on your readi...

Based on your reading of the article entitled "The Evolution of Color Vision", which of the following is a false statement? A. Trichomatic vision is found in all male and femal

Describe structure of biological compounds, Describe Structure of biologica...

Describe Structure of biological compounds? Structure :  The most important chemical compounds in living systems are probably the organic compounds. The term "organic" in the

Commensalism, #quesWhat benefits can commensalism offer to a species..

#quesWhat benefits can commensalism offer to a species..

Phosphorus cycle, Phosphorus cycle is sedimentary cycle because it does not...

Phosphorus cycle is sedimentary cycle because it does not enter into atmosphere. It can be found on earth in water, soil and sediments. The phosphorus cycle is the biogeochemical c

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd