cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Explain about precautionary principle, Q. Explain about precautionary princ...

Q. Explain about precautionary principle? The ethical values of biodiversity highlights the intrinsic value of biodiversity for its own sake and it is independent of the varied

Organs involved in the digestion process, The various organs involved  in...

The various organs involved  in  the digestion process are: Alimentary tract which  includes mouth, pharynx, esophagus stomach, small intestine, large intestine, appendix,  rec

What are primary consumers, What are primary consumers? Can a food chain pr...

What are primary consumers? Can a food chain present quaternary consumers without having secondary or tertiary consumers? Can a tertiary consumer of one chain be a primary or secon

Prevalence of coronaru heart diseases, Q. Prevalence of coronaru heart dise...

Q. Prevalence of coronaru heart diseases? Incidence of obesity, diabetes and CHD is increasing in India in almost all age groups. At present, obesity, which is a major risk fa

Which cells produced in first stage of embryonic development, Q. What are t...

Q. What are the cells produced in the first stage of the embryonic development called? The cells that result from the cleavage (the first stage of the embryonic development) ar

Disorders of lower respiratory tract, DISORDERS OF LOWER RESPIRATORY TRACT:...

DISORDERS OF LOWER RESPIRATORY TRACT: The lower respiratory  tract infections  tend to occur most frequently in young children who have not developed resistance  to infectious

How to alternately donating and accepting ions, Select all that are true or...

Select all that are true or correct regarding a buffer: alternately donating and accepting ions a set of chemicals that work together to resist pH change often a weak acid or base

Pox diseases, Pox diseases Small-pox in human beings and pox in a few ...

Pox diseases Small-pox in human beings and pox in a few animal species are closely related to each other. It is shown by the fact that the vaccine for the prevention of small

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd