cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

A leaf is detached from a tree and tested with iodine, A leaf is detached f...

A leaf is detached from a tree and tested with iodine. The leaf turns dark blue.    (a) What does this result tell you?    (b) Why is this result not sufficient e

Social determinants of health - social exclusion, Social Determinants of He...

Social Determinants of Health - Social Exclusion Given that absolute poverty is a major determinant of ill-health, the resultant social exclusion is ‘psychologically damaging,

Describe Fatty acids, In chemistry and particularly in biochemistry the fat...

In chemistry and particularly in biochemistry the fatty acid is a carboxylic acid with a long aliphatic tail (chain) that is either saturated / unsaturated. Many obviously occurrin

Explain the inferior dental nerve, Explain the inferior dental nerve T...

Explain the inferior dental nerve The inferior dental nerve in some cases may divide into two or three rami that occupy separate canals as the nerve travels in the mandible to

What is inharmonious ecological interaction, What is inharmonious ecologica...

What is inharmonious ecological interaction? Inharmonious, or negative, ecological interaction is that in which at least one of the participating beings is harmed.

Abscisic acid, Abscisic Acid Abscisic acid (ABA) as a naturally occurr...

Abscisic Acid Abscisic acid (ABA) as a naturally occurring growth inhibitor was discovered through independent investigations of different physiological phenomena in two diffe

Define water balance - hormonal control of fluid balance, Define Regulation...

Define Regulation of Water Balance - Hormonal Control of Fluid Balance? Hormonal control of fluid balance: When water intake is insufficient or water loss is excessive, the kid

#ti.., what would happen if there is no revolution?

what would happen if there is no revolution?

Effects of long term malnutrition, Effects of long term malnutrition ...

Effects of long term malnutrition The effects of long term malnutrition are more severe in children. Children do not have sufficient amounts of carbohydrates and fats in

Evolution of human kind, The evolution of human kind can be regarded as the...

The evolution of human kind can be regarded as the climax of phylogenic history of organisms. In the previous units of this block as well as the previous block we have detailed for

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd