cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Of which type of tissue are cartilages and bones made, Of which type of tis...

Of which type of tissue are cartilages and bones made? Are the cells of the connective tissue far or near to the others? The relative great spacing among cells is a typical

Nursing responsibilities while administering immunization, NURSING RESPONSI...

NURSING RESPONSIBILITIES WHILE ADMINISTERING IMMUNIZATION Use one sterile syringe and needle for each injection.  Use only the diluent supplied along with measles and

What is risk factor interaction, What is risk factor interaction ? Coro...

What is risk factor interaction ? Coronary artery disease, as has been explained, is a multifactorial disease with diverse risk factors coming together and interacting to produ

Define nucleosomes , The initial stage of packaging have the binding of t...

The initial stage of packaging have the binding of the chromosomal DNA to histones.  Whole, in chromosomes the ratio of the DNA to histones on a weight basis is around 1:1. There a

Atp molecules are produced for each glucose molecule, How many ATP molecule...

How many ATP molecules are produced for each glucose molecule used in fermentation? How many ATP molecules are produced for each glucose molecule used in aerobic respiration? I

True about microbial metabolism, which of the following is most likely to b...

which of the following is most likely to be true of microbial metabolism? 1)Acetyl-CoA is converted directly into 2 ATP 2)The energy from 2 ATP is necessary to drive the electron t

Coelenterata, is cnidaria having both interacellular & extracellular digest...

is cnidaria having both interacellular & extracellular digestion?

Homeostasis, why should shivering contribute to heat gain in the body?

why should shivering contribute to heat gain in the body?

Are plant growth is depend on the plant harmones, Are the growth and develo...

Are the growth and development of plants only influenced by plant hormones? Chemical and Physical environmental factors, like position and intensity of light in relation to the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd