cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Show the principles of prevention of diabetes mellitus, Q. Show the Princip...

Q. Show the Principles of Prevention of diabetes mellitus? Principles of primary prevention of diabetes are:     Creating awareness of diabetes mellitus   Identifying Pre-D

Electron transport and oxidative phosphorylation, Many of the free energy f...

Many of the free energy free during the oxidation of glucose to CO 2 is retained in the decreasing coenzymes NADH and FADH 2 producing while glycolysis and the citric acid cycle.

Renin-angiotensin system, As heart failure sets in, there is activation of ...

As heart failure sets in, there is activation of RAS (Fig 2.1). Adrenergic stimulation of beta-1 receptors in juxtaglomerular apparatus of the kidneys results in release of renin.

Caracterized by progressive loss of the axonal myelin sheath, Q. What are a...

Q. What are a few diseases characterized by progressive loss of the axonal myelin sheath? Multiple sclerosis is a severe disease caused by progressive destruction of the myelin

What are the intermediate hosts of plasmodium, What are the intermediate an...

What are the intermediate and the definitive hosts of the plasmodium? In the life cycle of the plasmodium humans are the middle hosts (where asexual reproduction takes place) a

How did gifford pinchot view nature, How did muir's impression of man's rel...

How did muir's impression of man's relationship with nature differ from Susan Fenimore coopers? And How did Gifford Pinchot view nature?

Vasodilatation from the time of injury, The blood vessels surrounding an ac...

The blood vessels surrounding an acutely inflamed site usually represent progressive vasodilatation from the time of injury.

Peripatric speciation, Peripatric Speciation Speciation by small populat...

Peripatric Speciation Speciation by small populations isolated on the periphery of the distribution of the parent population can be described as peripatric speciation. Small, pe

Effects of environmental disasters, Primary effects 1.      Nuclear con...

Primary effects 1.      Nuclear contamination 2.      Biological contamination 3.      Chemical contamination 4.      Destruction of infrastructure facilities. 5.

How parasitic infections are found, How Parasitic infections are found? ...

How Parasitic infections are found? Parasitic infections are found throughout the world. With increasing travel, immigration, use of immunosuppressive drugs and the spread of A

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd