cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Transport of gases in blood, Transport of Gases in Blood We know that ...

Transport of Gases in Blood We know that two types of gas exchanges are constantly occurring in the animal body--one at the inter phase of the respiratory membrane and externa

What is vegetarianism, What is Vegetarianism? Protein quality of the ...

What is Vegetarianism? Protein quality of the vegetarian diets can be improved by proper diet planning, However, under free-living conditions, vegetarianism can limit pr

Describe nerve cells, In eukaryotes possibly the most rapid and complex sig...

In eukaryotes possibly the most rapid and complex signaling is mediated through nerve impulses.  The Nerve  cells  (neurons)  consist  of  a cell  body  with  numerous projections

Which pancreatic tissues involved in endocrine secretion, Q. What are the p...

Q. What are the pancreatic tissues involved respectively in the endocrine and exocrine secretions? What are their respective hormones and enzymes? The exocrine secretion of the

Excretory system, What are the excretory organ in protozoa,coelenterates, i...

What are the excretory organ in protozoa,coelenterates, insects,spider,and arthropods

Define plan showing various steps of non-enzymatic browning, Define Plan sh...

Define Plan showing various steps of non-enzymatic browning? The plan showing the various steps of non-enzymatic browning is presented in figure Figure:

Why physical activity is important for weight loss, Why Physical Activity i...

Why Physical Activity is important for weight loss? You are already aware that exercise plays an important role in initiating and sustaining weight loss along with dietary and

Explain boiling point - properties of solution, Boiling Point There are...

Boiling Point There are certain properties of solutions  which are directly connected with vapour pressure and one of it is boiling point.  You must have observed that water bo

Heat exchangers, Normal 0 false false false EN-IN X-N...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

What are the nitrate reductase and nitrite reductase tests, Based on your u...

Based on your understanding of the nitrate reductase and nitrite reductase tests, why would the inclusion of nitrite inhibit anaerobic growth?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd