cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Explain differential staining technique, Explain Differential Staining Tech...

Explain Differential Staining Technique? It divides bacteria into separate groups based on staining properties, like Gram's stain, Acid-fast stain etc. Here 2 or more contrasti

State the red blood cells in the body capillaries in a leg, Which of the fo...

Which of the following will lead to a decrease in the amount of oxygen delivered to the body cells in a leg? A. A decrease in hydrogen ion concentration in red blood cells in t

Describe the structure of chromatin and chromosomes, Describe how the struc...

Describe how the structure of chromatin and chromosomes facilitates the following functions: a) DNA packaging. b) Regulation of gene expression.  c) Replication at the

Explain uristix benedicts test, Q. Explain Uristix Benedicts test? No s...

Q. Explain Uristix Benedicts test? No sugar/glucose is excreted in the urine in normal individuals. In diabetics, glucose is excreted by the kidneys when the blood sugar levels

Mitosis, explain mitosis in detail

explain mitosis in detail

Anabolism and catabolism, Anabolism and Catabolism Cellular metabolism...

Anabolism and Catabolism Cellular metabolism  has two aspects 1.      Anabolism  :  This aspect includes metabolic  process  by which  complex  cellular  compounds  are

Cell biology, what is the role of cholesterol in membrane fluidity?

what is the role of cholesterol in membrane fluidity?

Open and closed type of circulatory systems, Open and Closed Type of Circul...

Open and Closed Type of Circulatory Systems There are two categories of circulatory system found in higher metazoans. In one type the original blastocoel carries on to be the

What organisms make glycogen, What organisms make starch? What is it used f...

What organisms make starch? What is it used for? What organisms make glycogen? What is it used for?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd