cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

What would we conclude from pcr/dgge, What would you conclude from PCR/DGGE...

What would you conclude from PCR/DGGE (denaturing gradient gel electrophoresis) analysis of a sample that yielded one band by PCR and one band with DGGE? One band by PCR and two ba

Explain hormonal proteins, Explain Hormonal proteins Hormonal proteins ...

Explain Hormonal proteins Hormonal proteins coordinate the bodily activities. Various peptide and protein hormones (such as insulin and growth hormone) carry information tha

Explain nutritive and non-nutritive sweeteners, Q. Explain Nutritive and No...

Q. Explain Nutritive and Non-Nutritive Sweeteners? Nutritive Sweeteners: We know some sweeteners like glucose, honey, molasses, fruit juice, dextrose, maltose, mannitol, sorbit

Algae, classificationof algae

classificationof algae

Multidisciplinary approach to solving nutrition problems, Define Multidisci...

Define Multidisciplinary Approach to Solving Nutrition Problems? You must have realized by now that solving public nutrition problems represents a multidisciplinary challenge o

Theory of natural selection, What key idea, contained in Malthus's essay on...

What key idea, contained in Malthus's essay on populations, helped Darwin formulate his theory of natural selection?

Clinical manifestation and diagnosis of pericardiocentesis, Clinical Manife...

Clinical Manifestation Occurs over an extended time period and mimic those of CHF and corpulmonale. Dyspnea on exertion, dependent odema, ascitis, fatigue, anorexia and weight

What are the amphibian features, Q What are the amphibian features that mak...

Q What are the amphibian features that make them dependent on water to survive? Permeable skinexternal fecundation, body subject to dehydration, larval stage and eggs without s

What is gastrulation, Q. What is gastrulation? How during gastrulation are ...

Q. What is gastrulation? How during gastrulation are the first two germ layers formed? What are these germ layers? Gastrulation is the process through which a portion of the bl

Define the process of feeding 0-6 months infant, Define the Process of Feed...

Define the Process of Feeding 0-6 Months Infant? The term exclusive breast feeding is used when all fluids, energy and nutrients are provided by breast milk, with the possible

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd