cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Explain kingdom protista, Kingdom Protista The protistans are a complex a...

Kingdom Protista The protistans are a complex and diverse group of organisms that are placed together simply because they are all single celled eukaryotes but some form rather sp

Explain the basic working of cellular functioning, Q Which is the biologica...

Q Which is the biological molecule that holds the genetic information that is transmitted hereditarily and controls the cellular functioning? The hereditary molecule that contr

Ecology, (i) On Figure, draw a ring around the number which indicates the e...

(i) On Figure, draw a ring around the number which indicates the energy entering the system via photosynthesis. (ii) The total energy available to the plants in the ecosystem

Explain the principles of taxonomy, Explain the Principles of Taxonomy? ...

Explain the Principles of Taxonomy? The goal of taxonomy is to develop a workable classification that reflects evolutionary relationships and provides identification and no

Menstrual cycle- reproduction, Menstrual Cycle- Reproduction Menstrual...

Menstrual Cycle- Reproduction Menstrual cycles are characteristic of primates and do not occur in other vertebrate groups. The length of the cycle is highly variable, though 2

Agro industrial-mineral supplements, Mineral supplements There are two...

Mineral supplements There are two major classes of mineral sources: inorganic and organic. Organic complexes, however, have been shown to be more effectively absorbed by the a

Seed formation, Seed Formation A mature seed has seed coat, an embryo ...

Seed Formation A mature seed has seed coat, an embryo and reserve food material in the endosperm in monocots and in cotyledons in the dicots. Figure shows structure of dicots

Explain in brief about the neuropsychological test batteries, Explain in br...

Explain in brief about the neuropsychological test batteries Neuropsychological tests have the same standardisation requirements as all psychological tests. That is, there is t

The immediate effects of exercise on the heart and the lungs, (a) What are ...

(a) What are the immediate effects of exercise on the functions of (i) the heart, (ii) the lungs, (iii) the liver? (b) How do these changes help to meet the needs of exercise

Abrasive action of water and wind, Abrasive action of water, ice and wind ...

Abrasive action of water, ice and wind Water is an important mechanical weathering agent both in liquid as well as in the frozen state.  Rain water carrying sediments, ice glac

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd