cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Inorganic and organic substances, Q. Living beings are made of inorganic an...

Q. Living beings are made of inorganic and organic substances. According to the molecular complexity how can each of those substances be classified? Inorganic substances, mole

Why are the recombinant dna technology, Why are the recombinant DNA technol...

Why are the recombinant DNA technology and the nucleus transplantation technology still dangerous? A recombinant DNA technology and the nucleus transplantation technology (clon

Investigating, What is scientific investigation?

What is scientific investigation?

Inrinsic conduction system of the heart, The cardiovascular system controls...

The cardiovascular system controls the movement of blood through thousands of miles of capillaries so that every tissue  in every part of the body is perfused. Essential nutrients

Briefly explain about archenteron and blastopore, Q. What are the archenter...

Q. What are the archenteron and the blastopore? What is the stage of the embryonic development in which these structures are formed? What are the destinations of the archenteron an

Bacterial cells, which intracellular organelles are present in bacteria?

which intracellular organelles are present in bacteria?

What body system hiv impairs, Given that most AIDS victims die from overwhe...

Given that most AIDS victims die from overwhelming infections or rare types of cancer, what body system do you think HIV (the AIDS virus) impairs?

Illustrate wild life sanctuaries, Q. Illustrate Wild Life Sanctuaries? ...

Q. Illustrate Wild Life Sanctuaries? You must have seen how some of the parks are fenced. The intention is to keep the animals from wandering out and not to destroy neighbourin

Can you explain tannins, Q. Can you explain Tannins? Tannins are anoth...

Q. Can you explain Tannins? Tannins are another class of compounds which interfere with the absorption of minerals like iron and reduce the availability of proteins by bindin

Normal color vision- color blindness, If a color blind man marries a woman ...

If a color blind man marries a woman pure for normal color vision, it's probable that one of the below situations may result. Is it possible that a) All the children would be

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd