cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Will there be an opportunity to comment, Will there be an opportunity to co...

Will there be an opportunity to comment?  In a supplemental notice accompanying the last rule package, EPA is soliciting additional information and public comment on issues fro

Contagious ecthyma, Contagious ecthyma The contagious ecthyma virus is imm...

Contagious ecthyma The contagious ecthyma virus is immunologically distinct from vaccinia, but similar  to the causative agent of pseudo-cow-pox. It belongs to the genus Parapoxvi

Vertebrates, i need examples of animals that belongs to vertevrate pisces

i need examples of animals that belongs to vertevrate pisces

Explain the term - diffusion, Explain the term-Diffusion Diffusion occu...

Explain the term-Diffusion Diffusion occurs from the side of greater concentration to the side of lesser concentration. In aqueous humour this occurs from the plasma side in th

Types of disease, TYPES OF DISEASE    ...

TYPES OF DISEASE     communicable Caused by infective agents disease

Biosecurity in poultry health management, B i osecurity in Poultry Health...

B i osecurity in Poultry Health Management Biosecurity includes all the measures that are taken to prevent infection to individual, material and environment from the pathogen

Osmotic function - essential elements, Osmotic Function - Essential Element...

Osmotic Function - Essential Elements Plant cells generally contain mineral ions 10 to 1000 times higher in concentration than the surrounding soil. That is why water enters t

Define reagents for measurement of ph, Define Reagents for Measurement of p...

Define Reagents for Measurement of pH? Buffer solutions of pH 4, pH 7 and pH 9.18 for calibration and an unknown solution (i.e. solution of unknown pH) You can prepare these

Ventricular assist devices in circulatory assist devices, Ventricular Assis...

Ventricular Assist Devices :  These come handy when IABP has failed or when prolonged circulatory support is needed. Now Ieft ventricular (LVAD), right ventricular (RVAD) and bive

Muromonab cd-3 , Muromonab CD-3 (Orthoclone, OKT-3) A monoclonal anti...

Muromonab CD-3 (Orthoclone, OKT-3) A monoclonal antibody against mature T lymphocytes, OKT-3 is primarily used to treat acute rejection episode. IV for 10-14 days. Effe

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd