cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Explain about harvesting autogenous bone grafts, What are the precautions t...

What are the precautions to be taken when harvesting autogenous bone grafts from mandibular symphysis and ramus? When harvesting grafts from the mandibular symphysis region, fo

Define eggs as a rich source of protein, Define Eggs as a rich source of pr...

Define Eggs as a rich source of protein? Roughly, the chicken egg consists of 11% shell, 31% yolk and 58% white. Liquid whole egg consists of 65% white and 35% yolk. The primar

Discuss about smooth muscles, Smooth muscles These muscles are beyond o...

Smooth muscles These muscles are beyond our control. That means these muscles are controlled by autonomic nervous system. They are non-voluntary muscles. They are found in thin

Diseases related with kidney, DISEASE CONCERNED WITH KIDNEY - 1.      P...

DISEASE CONCERNED WITH KIDNEY - 1.      Paget's disease - Abnormalities of kidney. 2.      Uramia - Increases in quantity of urea. 3.      Nephritis - Inflamation in glom

Write the meaning of polydypsia, Q. Write the meaning of Polydypsia? In...

Q. Write the meaning of Polydypsia? Increased Thirst (Polydypsia): As the patient is losing more water through excessive urination, the patient feels thirstier and tends to tak

Explain about the tray and tunnel dryers, Explain about the Tray and Tunnel...

Explain about the Tray and Tunnel Dryers? Tray and tunnel dryers are widely used in the food industry. In both cases the material being dried is supported on multiple trays, wi

Marking regulations of honey, Q. Marking regulations of honey? The mar...

Q. Marking regulations of honey? The marking regulations indicate that each container of honey shall be suitably marked so as to give suitable information as follows: a) N

Explain the principle of nelson-somogyi method, Explain the Principle of Ne...

Explain the Principle of Nelson-Somogyi Method? Glucose is estimated by Nelson-Somogyi method. We begin our study of this method by getting to know the principle involved in th

Define the meaning of behaviour change communication, Meaning of Behaviour ...

Meaning of Behaviour Change Communication Now day's very common term used for giving health education to people and for giving information to bring change in behaviour is Behav

Biochemical nutrient cycle, What are some ways that humans can reduce the e...

What are some ways that humans can reduce the effect they have on the phosphorus cycle?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd