cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Define the causes of failures of root canal treatment, Define the Causes of...

Define the Causes of Failures of Root Canal Treatment 1. Errors in diagnosis and treatment planning 2. Coronal leakage ( inadequate restorative protection) 3. Lack of kno

Spinal nerves, Spinal nerves: They take their origin from the spinal...

Spinal nerves: They take their origin from the spinal cord. They are the mixed nerves having both sensory and motor fibres. There are 31 pairs of spinal nerves. The se

Human biology , Your final assignment is to write an (~2000 words) on any d...

Your final assignment is to write an (~2000 words) on any drug that is currently approved by the FDA, describing how the drug works based on the mechanisms of human biology describ

Inheritence, What is inheritence and what is the relation between mendel an...

What is inheritence and what is the relation between mendel and darwin?

Phylogenetic species concept, Q. Phylogenetic species concept ? Taxonom...

Q. Phylogenetic species concept ? Taxonomists (scientists who compare, classify and name organisms) tend to describe a species by studying their physical characteristics and be

What factor cause the measurement to change, During gym class Sally noticed...

During gym class Sally noticed that her friend Melissa always ran faster than her. Sally knew that they exercised equally, so she wondered what could cause Melissa to run so fast.

Circulation, Circulation, Respiration and Excretion of Asteroidea Aste...

Circulation, Respiration and Excretion of Asteroidea Asteroids depend upon the circulation of coelomic fluid for the transport of gases and some nutrients. The blood vascular

Endosperm, 1. What are the examples of helobial endosperm

1. What are the examples of helobial endosperm

Express an example of directional selection, The peppered moth experiment w...

The peppered moth experiment was an example of directional selection; due to environmental conditions the directional selection shifted from one extreme to the other in 1850s Engla

Explain difference between fats and oils, Explain the molecular difference ...

Explain the molecular difference between fats and oils. How do the differences affect their physical properties?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd