cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Determine the term - chlorine, Determine the term - Chlorine   The appl...

Determine the term - Chlorine   The application of chlorides results in leaves of greater spread, a lighter green colour and with a smoother surface. However, the combustibilit

What is the climax stage of an ecological succession, What is the climax st...

What is the climax stage of an ecological succession? The climax stage is the stage of the ecological succession in which the community of an ecosystem becomes stable and does

In what order should personal protection be removed, Q. In what order shoul...

Q. In what order should Personal Protection be removed? Personal protection is removed in following order: Gloves Mask Glasses Wash hands

Determine energy density of human milk, Determine Energy Density of Human M...

Determine Energy Density of Human Milk? The energy density of human milk depends on the relative proportions of protein, fat and the principal carbohydrate, lactose. Lactose co

Cells, what are golgi apparatus

what are golgi apparatus

Etenobotany, Ethnobotany : This is the study of relationship between tribal...

Ethnobotany : This is the study of relationship between tribals and plants. Ethnobotany is from "ethnology" - study of culture or "botany" - study of plants. This is the scientific

Respiration in scorpion or spider, RESPIR A TIO N IN SCORPION OR SPIDER ...

RESPIR A TIO N IN SCORPION OR SPIDER - Respiratory organs are book lungs. A book lung is a chamber containing a series of thin, vascular, parallel lamellae arranged li

Requirements and recommended dietary allowances (rda), Define Requirements ...

Define Requirements and Recommended Dietary Allowances (RDA)? What do we mean by requirements and RDA? The requirement level is the amount of nutrient needed to be absorbed to

Explain the interaction of vitamin a with zinc, Explain the interaction of ...

Explain the interaction of vitamin a with Zinc? Its deficiency interferes with vitamin A metabolism. It leads to a reduction in the synthesis of plasma proteins, particularly,

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd