cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Why are vaccines used in the prevention of infections, Why are vaccines use...

Why are vaccines used in the prevention but not in the treatment of infections? Why can antivenom serums be used in prevention and treatment? Vaccines are not used in the treat

Parasites - biological stress, Parasites - Biological Stress Many inse...

Parasites - Biological Stress Many insects and microorganisms can feed upon tissues and saps of living plants. Hence plants must be protected against such parasites either by

Define standard or total plate counts (spc/tpc), Define Standard or Total P...

Define Standard or Total Plate Counts (SPC/TPC)? It is the most widely used method to know the microbiological quality of the food sample. It is quick and efficient method, giv

RDA.., formulation of RDA

formulation of RDA

Physiological & socio psychological factor- public nutrition, Define Physio...

Define Physiological and Socio Psychological Factors - public nutrition? Food related behaviour depends on a combination of biochemical factors, mainly, physiological aspects a

How is price mechanism or supply and demand concerned, How is price mechani...

How is price mechanism or supply and demand concerned?   The price mechanism or supply and demand: The price mechanism or supply and demand is concerned with how buyers

What are holandric genes, What are holandric genes? The Holandric genes...

What are holandric genes? The Holandric genes are genes situated in the nonhomologous region of the Y chromosome. Holandric genes condition phenotypes that emerge only in men s

Define drawbacks for underwater weighing method, Define drawbacks for under...

Define drawbacks for underwater weighing method? Since the major drawbacks with UWW method are that the subject needs to be totally submerged underwater and exhale all of the a

Equipment of circulatory assist devices, Equipment :  Basically, it has an...

Equipment :  Basically, it has an intra aortic balloon pump. A balloon with a capacity of 40 ml is passed percutaneously through the femoral artery up to the upper end of descen

Main prophylactic measures against hookworm disease, Q. What are the main p...

Q. What are the main prophylactic measures against hookworm disease? The major prophylactic measures against hookworm disease are to avoid walking barefoot on soils suspected o

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd