cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Explain difference between monosaccharides and disaccharides, What is the d...

What is the difference between monosaccharides and disaccharides? What are some examples of disaccharides and of monosaccharides that form them? Monosaccharides are simple mole

Slim mold, what is the cycle of slime moldand thier structure

what is the cycle of slime moldand thier structure

What gases was the earths primitive atmosphere, Before the emergence of lif...

Before the emergence of life of what gases was the earth's primitive atmosphere constituted? The earth's primitive atmosphere was basically produced of methane, hydrogen, ammon

Explain the role in insulin formation, Explain the Role in Insulin Formatio...

Explain the Role in Insulin Formation? You are aware that insulin receptors are present in many cells with their concentration being highest in adipocytes (cells present in adi

Explain the toxicity of nicotinic acid, Explain the Toxicity of nicotinic a...

Explain the Toxicity of nicotinic acid? Although therapeutically useful in lowering serum cholesterol, administration of chronic high oral doses of nicotinic acid can lead to h

Where in living beings can found mineral salts, Q. Which are mineral salts?...

Q. Which are mineral salts? Where in living beings can found mineral salts? Ans. Mineral salts are simple inorganic substances made of metallic chemical elements, like sodiu

Spoilage by fungi and bacteria, Q. Explain Spoilage by fungi and bacteria? ...

Q. Explain Spoilage by fungi and bacteria? The dominant spoilage organisms in fruits are fungi, as their pH is low (normally 5),whereas the pH of vegetables is fiom 5.0 to 7

Explain healthcare burden of heart diseases, Explain Healthcare Burden OF H...

Explain Healthcare Burden OF HEART DISEASES? CAD and its complications use up a substantial portion of the scarce healthcare resource of the country. It has been reported that

What are the steroids, Q. What are the steroids? What are the few examples ...

Q. What are the steroids? What are the few examples of steroids with a biological function? Steroids are lipids based in an angular combination of four carbon rings, one ring m

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd