cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Post-operative care of teeth, Post-operative care of teeth After implan...

Post-operative care of teeth After implant surgery, patients should be warned to expect:  Some swelling and possibly bruising.  Some discomfort which can usually be contr

Typical components of a closed circulatory system, Q. What are the typical ...

Q. What are the typical components of a closed circulatory system? The typical components of the closed circulatory system are the blood vessels within which blood circulates c

Objectives of the nutritional care process, Thus, the objectives of the nut...

Thus, the objectives of the nutritional care process should include the following points: 1. Restoration of good nutritional status with dietary modifications and counseling.

Illustrate the aqueous phase in sol processing, Illustrate the aqueous phas...

Illustrate the aqueous phase in sol processing In a sol possessing a continuous aqueous phase, the colloidal particles have an electrically charged surface.  The ionized groups

What are the maintenance sequences of implant, Maintenance sequences of imp...

Maintenance sequences of implant Achieving a successful result with the treatment is only half the battle won. The main challenge is maintain that result over a long period of

How to prevent or overcome this nutritional deficiency, How to prevent or o...

How to prevent or overcome this nutritional deficiency?  A large number of government schemes and programmes have been launched about which we have already studied in the Publi

Describe the theory of darwins, Describe the theory of Darwin's? Most s...

Describe the theory of Darwin's? Most scientists today accept the theory that living organisms have evolved according to the concept that was first proposed by Charles Darwin i

Acyanotic congenital heart disease, Acyanotic Congenital Heart Disease   ...

Acyanotic Congenital Heart Disease   In this infant has no cyanosis because there is no abnormal communication between pulmonary and systemic circulation or there  is no mixing

Explain phylum-bacteria, Phylum-Bacteria (Eubacteria) Eubacteria,th e t...

Phylum-Bacteria (Eubacteria) Eubacteria,th e true bacteria, are extremely small and they exist in vast numbers virtually everywhere on earth. They range from the upper atmosphe

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd