cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Population, Population You must be familiar with the term 'population'. I...

Population You must be familiar with the term 'population'. It is one of the most talked about issues of this century. It is feared that the rapid growth of the world population

Conservation of biodiversity, Conservation of biodiversity is very importan...

Conservation of biodiversity is very important to maintain the ecological balance of ecosystem. Various steps for conservation of biodiversity: 1.           Creation of consc

Anabolism and catabolism, Anabolism and Catabolism Cellular metabolism...

Anabolism and Catabolism Cellular metabolism  has two aspects 1.      Anabolism  :  This aspect includes metabolic  process  by which  complex  cellular  compounds  are

Functions of citric acid cycle, Functions of Citric Acid Cycle The citr...

Functions of Citric Acid Cycle The citric acid cycle is an amphibolic pathway  i.e.  it  is involved in both anabolic and catabolic processes

What are the endocrine glands, What are the endocrine glands that regulate ...

What are the endocrine glands that regulate sexual activity in males? How does this regulation work and what are the involved hormones? In males the sexual activity is regulate

Combination of meat products, C o mbination of meat products Combinin...

C o mbination of meat products Combining meats and byproducts of different species to produce value added products results in complementation and supplementation of their qua

Define working of body surface in excretion of zinc in human, Define workin...

Define working of Body surface in excretion of zinc in human? Loss of zinc occurs due to the exfoliation of skin and sweating (0.7 -1.0 mg/day). Another route of zinc loss is h

Define different uses of additives in food, Normal 0 false fa...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Define lipids - tests for presence of exoenzymatic activity, Define Lipids ...

Define Lipids - Tests for Presence of Exoenzymatic Activity? Lipids are also high molecular weight compounds. Enzyme lipases (esterases) cleaves the ester bond to form glycerol

Pathophysiology, Case scenario Mr Kit Lee, a 28-year-old unrestrained dr...

Case scenario Mr Kit Lee, a 28-year-old unrestrained driver of a utility, was travelling on a highway at 100kph and swerved to miss road debris. He lost control and struck a tre

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd