cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Obelia, Obelia is considered to be of special interest in zoology as an ani...

Obelia is considered to be of special interest in zoology as an animal showing an intermediate grade of organization?

Planning of nursing care - hypospadias, Planning of Nursing Care Planni...

Planning of Nursing Care Planning of the nursing care depends on the decision of the surgery. The surgery may be performed in two stages depending upon the degree of the d

Parturition , P ARTURITIO N - Gestation period is of 280 days (in rab...

P ARTURITIO N - Gestation period is of 280 days (in rabbit 28 days). Parturition is the expelling of fully mature young ones from the mother's uterus. Oxytocin stimulates

Is crossing over important diversity of biological evolution, Is crossing o...

Is crossing over important for the diversity of biological evolution? The Sexual reproduction and the recombination of linked genes (crossing over) are, along with mutations, t

Definition of osseointegration in microscopic biophysical, Q. Definition of...

Q. Definition of Osseointegration in microscopic biophysical? Osseointegration implies that at light microscopic and electron microscopic levels, the identifiable components of

Effects on aquatic organisms - water pollution, Effects on Aquatic Organism...

Effects on Aquatic Organisms - Water Pollution Toxic water pollutants such as metals, pesticides, insecticides and chemicals affect aquatic species directly whereas the non-t

Enzymes, Biological catalysts à speed up chemical reactions in the body wit...

Biological catalysts à speed up chemical reactions in the body without being consumed in the process Reduce the activation energy needed for reactants to reach the transit

Define population size, Define Population size, inbreeding and genetic vari...

Define Population size, inbreeding and genetic variation? "Darwin proposed, and modern evolutionary biology affirms, that evolution is relies on variation in the characteristic

Baby delivery, some deliveries of baby occur early na. y is it so?

some deliveries of baby occur early na. y is it so?

Why the av node of a mammalian heart is destroyed, The AV node of a mammali...

The AV node of a mammalian heart is destroyed. A. A depolarization in a cell in the left atrium will cause a depolarization of a cell in the left ventricle. B. The contracti

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd