cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Amoeboid movement, Normal 0 false false false EN-IN X...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Explain precautions for preparation of bacterial smear, Explain Precautions...

Explain Precautions for Preparation of Bacterial Smear? 1. Plates used should be prepared 24 hours before use to avoid spreading of colonies due to moisture in plates. 2. Sw

Thermal relations, Normal 0 false false false EN-IN X...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Structure of myofibril, STRUCTUR E OF A MYOFIBRIL - The dark bands ...

STRUCTUR E OF A MYOFIBRIL - The dark bands of the myofibril are termed the A-bands (Anisotropic bands). Each A-band has at its middle a light zone called H-zone (Henson'

What is gene expression, What is Gene Expression ? Gene expression i...

What is Gene Expression ? Gene expression is the process by which a gene is activated to produce a certain protein. Cells are able to respond to changes in the environment i

Protection of protein meals or bypass protein, Protection of protein meals ...

Protection of protein meals or bypass protein The protein of oil cakes, having higher protein degradability, such as groundnut cake, mustard cake are utilized in the rumen les

Determine the uses of electrical recording - brain function, Determine the ...

Determine the uses of Electrical Recording - Brain function We can also learn about brain function by recording its electrical activity. In electroencephalography (EEG) and the

Diversity is good axioms of conservation biology, "Diversity is good" is on...

"Diversity is good" is one of the axioms of conservation biology. However in absentia of historical information, why is noting higher diversity in a system NOT the best way to eval

ATP FORMATION, what compound is phosphorylated for ATP? what is the resulti...

what compound is phosphorylated for ATP? what is the resulting compound when ATP releases energy

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd