cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Explain android obesity, Normal 0 false false false EN-...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Explain the lingual nerve and artery, Lingual nerve and artery It is th...

Lingual nerve and artery It is the branch of mandibular nerve which enters the oral cavity above the posterior edge of the mylohyoid muscle close to the 3 rd molar region proc

Interrupted suture technique-prosthetic aortic valve, Interrupted suture te...

Interrupted suture technique: Synthetic braided 2 '0' sutures (ethibond) with 17mtn needles on either end and a pledget is used. Alternate green and white sutures are taken aft

Diagnosis of acute myelogenous leukemia, The most recent blood work of a pa...

The most recent blood work of a patient with a diagnosis of acute myelogenous leukemia (AML) reveals thrombocytopenia. Where is the patient most likely to experience abnormal bleed

Explain other lipids in biological compound, Explain Other lipids in biolog...

Explain Other lipids in biological compound? Other Lipids :  Another important type of lipids are the steroids, with a basic structure consisting of four interconnecting carb

Responses of plants to stress, Responses of Plants to Stress You know ...

Responses of Plants to Stress You know that certain plant species can grow in severe environmental extremes. For example, plants grow below 0°C in the Himalayas and above 45°C

State the term - migraine stroke, State the term - Migraine Stroke Sinc...

State the term - Migraine Stroke Since the late 1800s, physicians have recognised that migraine attacks may lead to infarcts and permanent neurological deficits. Such migraine

Explain the mechanisms suggested for bile acid adsorption, Explain the Mech...

Explain the Mechanisms suggested for bile acid adsorption? Mechanisms suggested for bile acid adsorption are: 1. Hydrophobic interactions between lignin and bile acids, 2

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd