cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Define the healing process - root canal treatment, Define the Healing Proce...

Define the Healing Process - Root Canal Treatment The course of healing varies and depends on: 1)  Initial size of the lesion: It takes longer for larger periradicular lesio

Mechanical isolation, In certain instances differences in the morphology of...

In certain instances differences in the morphology of genitalia between species make it impossible for normal mating to occur, and sympatric species tend to remain isolated. When t

Nursing education, Nursing  Education   Computer-Assisted Instruction ...

Nursing  Education   Computer-Assisted Instruction (CAI) is a valuable teaching aid for health care students, testing them with frequent quizzes to ensure that the material rea

Explain the process of metastasis, Explain the process of metastasis. Defin...

Explain the process of metastasis. Define anti-angiogenic drugs and relate the role of medical imaging, if any, in anti-angiogenic drug therapy. State how you envision your particu

Determine the term - origin of life on earth, Based on your reading of the ...

Based on your reading of the article "Origin of Life on Earth" which of the following is a false statement regarding how life developed? A. As proteins are needed for the repli

Determine the parathryroid hormone receptors, Which of the following serves...

Which of the following serves as an effector, or part of an effector, that functions in a negative feedback system? A. 1,25-dihydroxyvitamin D Receptors located intracellularly

What is syphilis, What is syphilis? Syphilis, also known as lues, is a ...

What is syphilis? Syphilis, also known as lues, is a disease caused by the bacteria Treponema pallidum. Before the discovery of penicillin, syphilis was a fatal disease. Today

Taxonomy, how do i classify mythical creatures into a web?

how do i classify mythical creatures into a web?

Heavy metals require for the normal finctioning of our body, Q. Heavy Metal...

Q. Heavy Metals require for the normal finctioning of our body? Like the foods we consume, our bodies also contain many metallic elements. Most of these metals are actually r

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd