cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

What does radial symmetry mean, What does radial symmetry mean? What is the...

What does radial symmetry mean? What is the type of symmetry found in chordates? Which are other phyla of the animal kingdom that present species with radial symmetry? Radial

Microbiology, what are the two alternate pathways to glycolysis

what are the two alternate pathways to glycolysis

What is counsellor, Q. What is Counsellor? Counsellor is someone who gi...

Q. What is Counsellor? Counsellor is someone who gives advice about the problem. A counselor's role is to help patients help themselves. In our context  a counsellor is  a comm

Determine the evaluation of the bone, Evaluation of the bone Evaluation...

Evaluation of the bone Evaluation of the bone -implant interface needs to be done by the following: Failures in Implant Dentistry 1) Implant mobility and discomfort 2)

Can two organisms share the similar kingdom, If two organisms share the sim...

If two organisms share the similar kingdom, must they also share the similar domain? Explain. Two organisms that share the similar kingdom must also share the similar domain b

Why and how does the food get spoiled, Why and how does the food get spoile...

Why and how does the food get spoiled? There are several causes of food spoilage. These include: Growth of Microorganisms, such as bacteria, molds and yeasts, which can

Define methods for determining amount of microbes in food, Define Methods f...

Define Methods for Determining the Amount of Microbes in Food? Following methods can be employed for determining the amount of microbes in the food samples and analyzing their

Zonation in the ocean, Q. Zonation in the ocean? The oceans play a majo...

Q. Zonation in the ocean? The oceans play a major role in determining the climate and sustaining life on earth. Oceans help to redistribute the solar energy, through ocean curr

What does secondary productivity in an ecosystem indicate, a) What does sec...

a) What does secondary productivity in an ecosystem indicate? b) List any two factors by which productivity is limited in aquatic ecosystems

What are alleles of a gene, What are alleles of a gene? Diploid individ...

What are alleles of a gene? Diploid individuals have paired chromosomes. For instance in humans there are 23 pairs of chromosomes totalling 46 chromosomes. Each pair comprehend

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd