cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Fate of pyruvate, Pyruvate Pyruvate has three different  fates. Under a...

Pyruvate Pyruvate has three different  fates. Under aerobic conditions,  pyruvate enters mitochondriaand is converted to acetyl CoA. The acetyl CoA enters thecitric acid cycle.

Ecological pyramids - ecosystem, Ecological Pyramids - Ecosystem The a...

Ecological Pyramids - Ecosystem The ancient Egyptians constructed elaborate tombs called pyramids. The base of the pyramid is broad and it supports the upper levels of the str

Respiration – protozoan, Respiration – Protozoan Gas exchange occurs b...

Respiration – Protozoan Gas exchange occurs by the diffusion of oxygen across the cell membrane. Some protozoan utilize this oxygen but are also capable of anaerobic respirati

Explain stress and psychosocial tension in lifestyle risk, Explain Stress a...

Explain Stress and Psychosocial Tension in lifestyle risk factors? Stress and psychosocial tension are other lifestyle factors in initiating coronary artery disease and precipi

Signs of constrictive pericarditis, Q. Signs of constrictive pericarditis? ...

Q. Signs of constrictive pericarditis? 1) Jugular venous pressure (JVP) is elevated in all patients of CP which reflects the elevated diastolic pressure of the right atrium.

Prevention of iron deficient anaemia, Prevention   You as nurse needs t...

Prevention   You as nurse needs to ensure that iron supplementation  is given to children who are at risk for iron deficiency.  Iron supplementation  should start between 4 and

Hypothalamus gland, HYPOTHALAMUS - Hypothalamus develops from the ectod...

HYPOTHALAMUS - Hypothalamus develops from the ectoderm of the embryo. Location and Structure. It lies below or inferior to the thalamus. The hypothalamus is connected to t

Determine the term anapsid , Determine the term Anapsid ? The type of s...

Determine the term Anapsid ? The type of skull found in reptiles and seen today only in turtles. Behind the opening of the orbits the skull is solid and lacks temporal openings

Use of toxic amino acids, Q. Explain Use of Toxic amino acids? Lathyru...

Q. Explain Use of Toxic amino acids? Lathyrus sativus (kesari dhal) seeds contain toxic amino acid beta-oxalyl aminoalanine (BOAA), is considered to be responsible

Does the molecule will remain unchanged, A double-stranded piece of DNA, 10...

A double-stranded piece of DNA, 100 base pairs in size, is exposed to a high pH environment. This causes all non-valent interactions to be disrupted. What will result from this tre

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd