cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Explain the treatment options after endodontic failure, Explain the Treatme...

Explain the Treatment Options after Endodontic Failure a) Non-surgical retreatment "re-endo", b) Root-end surgery "if there is a will detected mistake as separated instrumen

Explain the control of air pollution, Explain the Control of Air Pollution ...

Explain the Control of Air Pollution a. Air belongs to everyone Need it to treat air pollution as public problem   b. Air pollution is an inevitable concomitan

What does a two-direction arrow show in a chemical equation, What does a tw...

What does a two-direction arrow show in a chemical equation? The oxygen atoms share two pairs of electrons, as every atom needs two more electrons to fill the orbitals of its o

Total artificial heart in circulatory assist devices, Total Artificial Hear...

Total Artificial Heart :  Jarvik seven was the first successful total artificial heart supporting the patient for 112 days. The disadvantage is that the patient has to be connecte

Define about the centrifuge, Define about the Centrifuge? Centrifuge is...

Define about the Centrifuge? Centrifuge is an apparatus consisting essentially of a compartment spun about a central axis to separate contained materials of different specific

Explain treatment options for ailing and failing implants, Treatment option...

Treatment options for ailing and failing implants are varied. The clinician should start conservatively and progress to more aggressive therapy. The overall goal of therapy is

Determine the significance of mesoglea, Determine the significance of Mesog...

Determine the significance of Mesoglea. The jellylike layer found between endodermal and ectodermal cell layers of diploblastic organisms. It acts as a type of cement holding t

When aflatoxigenic molds can occur, Q. When Aflatoxigenic molds can occur? ...

Q. When Aflatoxigenic molds can occur? Occurrence: Aflatoxigenic molds can occur in warmer parts of the world and aflatoxicosis maybe produced in a wide range of tropical and s

Define anaemia in pregnancy, Define Anaemia in Pregnancy? Anaemia is th...

Define Anaemia in Pregnancy? Anaemia is the most common complication and is often compounded by low socio- economic status. However, it is seen frequently in upper income women

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd