cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

THC and testis weight., A study set out to determine whether tetrahydrocan...

A study set out to determine whether tetrahydrocannabinol (THC, the psychoactive component of marijuana) affects fertility in male hamsters. Two different experiments were conducte

Suppose that the substance does not ionize, Percent solutions are made on t...

Percent solutions are made on the basis of g/ml or ml/ml of diluent, and it is usually assumed that 1 ml of water = 1 g of water. For example, 20 g/100 ml of water or 200 g/1000 ml

Explain total anomalous pulmonary, Explain Total Anomalous pulmonary Venous...

Explain Total Anomalous pulmonary Venous Connection (TAPVC) ? In this congenital anomaly there is no direct communication between the pulmonary veins and the left atrium. All

Explain crossing over in genetics, Explain Crossing Over in genetics? C...

Explain Crossing Over in genetics? Crossing over is when the arms of homologous chromosomes exchange segments (and therefore genes) with each other. Recall that one of the homo

What are the types of neurons, According to the function of the transmitted...

According to the function of the transmitted neural impulse which are the types of neurons? How different are the concepts of afference and efference of the neural impulse transmis

Draw a diagram of transverse section of the spinal cord, Draw a diagram of ...

Draw a diagram of transverse section of the spinal cord showing reflex are and label the following parts: (i) Afferent nerve fibre (ii) Grey matter (iii) Motor nerve fibre

Define hepatic cholesterol synthesis, Q. Define Hepatic Cholesterol Synthes...

Q. Define Hepatic Cholesterol Synthesis? This is a highly complex process beginning with acetyl CoA formed from fatty acid oxidation or from carbohydrate breakdown. The rate-de

Define atherosclerosis, Q. Explain Atherosclerosis? Atherosclerosis is ...

Q. Explain Atherosclerosis? Atherosclerosis is one of the most important causes of morbidity and mortality in developed as well as in developing countries. Atherogenesis occurs

Diseases caused by abnormal gh secretion by the hypophysis, Q. What are som...

Q. What are some diseases caused by abnormal GH secretion by the hypophysis? In childhood deficient GH secretion may lead to delayed growth and in severe cases to nanism that i

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd