cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

How do sponges try to protect themselves against harm, How do sponges try t...

How do sponges try to protect themselves against harm from the environment? Is that method efficient or rudimentary? Sponges can close their pores to avoid the entrance of wate

Define the role of riboflavin in drug and lipid metabolism, Define the role...

Define the role of riboflavin in Drug and lipid metabolism? Flavoproteins catalyze dehydrogenation reactions, as well as, hydroxylations, oxidative decarobxylations, deoxygenat

Adaptive Radiations, Adaptive Radiations The organisms all the time te...

Adaptive Radiations The organisms all the time tend to establish harmony with their environment. This process, called adaptation, enables the organism to face the vagaries of

Why pluricellular organisms are produced by serial mitosis, From the zygote...

From the zygote, pluricellular organisms are produced by serial mitosis. Would this formation be possible if every cell made by mitosis had an identical life in relation to its ant

Drugs derived from plants, A large number of important drugs are derived fr...

A large number of important drugs are derived from plants. For example Tubocuranin, derived from plant-based curare, is used as a muscle relaxant during surgery. Curianol, a Guy

Explain about the dietary reference intakes, Explain about the Dietary Refe...

Explain about the Dietary Reference Intakes? Dietary Reference Intakes (DRIs) are relatively new to the field of nutrition. The DRIs are a set of four nutrient-based reference

Widal test is used for the diagnosis, Widal test is used for the diagnosis ...

Widal test is used for the diagnosis of: 1. Malaria 2. Pneumonia 3. Tuberculosis 4. Typhoid Typhoid

Protein necessity, Proteins necessity They are called as body builde...

Proteins necessity They are called as body builders. The proteins are used in various metabolic pathways and body building. They help in repairing the tissues of the b

Explain the cell division, What is the cell division during the first stage...

What is the cell division during the first stage of the embryonic development called? How is this stage characterized? The cell division in the first stage of the embryonic de

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd