cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Molecule synthesis and molecule break-down, Match dehydration reaction and ...

Match dehydration reaction and hydrolysis reaction to molecule synthesis and molecule break-down

Homologous structures, Homologous structures are the body parts in differe...

Homologous structures are the body parts in different organisms which have similar bones and similar arrangements of the blood vessels, muscles, and nerves and go through similar

Vitamin - a, VITAMIN - A Discovered by Macollum. Also known as ...

VITAMIN - A Discovered by Macollum. Also known as Retinol / Antixeropthalmic vitamin / Auxerophthol. It is vitamin for normal vision. It forms retinal pigments rho

Class of crustacea - cirripedia, Class of Crustacea - Cirripedia These...

Class of Crustacea - Cirripedia These crustaceans are completely marine, and include the barnacles. Moa species are free living, attached to rock, and other objects. Some are

Examples of invertebrate nervous system, Q. Using examples of invertebrate ...

Q. Using examples of invertebrate nervous systems how can the process of evolutionary cephalization be described? Considering the example of invertebrates it is observed that e

How simple transposition of the great arteries present, How Simple Transpos...

How Simple Transposition of the Great Arteries Present after 30 Days ? A switch operation will have poor results, as the ventricle that has regressed will not be able to mainta

Can you explain secondary metabolites, Q. Can you explain Secondary Metabol...

Q. Can you explain Secondary Metabolites? Secondary metabolites or secondary plant products are those macromolecules that lack nitrogen and arc of restricted occurrence and the

Define protein requirement at different stages of life cycle, Define Protei...

Define Protein Requirement at Different Stages of Life Cycle? Methods of Estimating and Assessing Protein Requirements at Different Stages of Life Cycle In this section, we

What is transgenesis - molecular biology, Molecular Biology - transgenesis ...

Molecular Biology - transgenesis Difficult to successfully insert the gene then successfully re-implant embryo possible pleiotropic effects low success rate / tak

In which field dietetics related, The field of  dietetics can be related to...

The field of  dietetics can be related to: Nutrition care and intervention focused on the individual, and Nutrition care and intervention focused on the group.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd