cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

How are sponges characterized, Q Sponge identity card. How are sponges char...

Q Sponge identity card. How are sponges characterized according to example of representing beings, basic morphology, type of symmetry, embryonic (germ) layers and coelom, digestive

What are the most important worm infections, Q. What are the most important...

Q. What are the most important worm infections? The most significant human worm infections are taeniasis, schistosomiasis, cysticercosis, ascariasis and ancylostomiasis (hookwo

Find no. of pair of alleles involved polygenic inheritance, How to find the...

How to find the number of pair of alleles involved in polygenic inheritance using the number of phenotypical forms of the trait they condition? Considering the "p" number of th

Malpighian tubules of insects, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Write in 5-6 lines about food pyramid, Write in 5-6 lines about food pyrami...

Write in 5-6 lines about food pyramid. The diabetes food pyramid divides food into six groups. These groups vary with each other on the basis of si-e occupied in the pyramid.

Explain the adverse effects of menactra, Adverse effects of menactra The...

Adverse effects of menactra The most common adverse reactions to Menactra include headache, fatigue and malaise, in addition to pain, redness and induration at the site of injec

Why dietary supplements do not speed up a child''s growth, Why Dietary supp...

Why Dietary supplements do not speed up a child's growth? Dietary supplements do not "speed up" a child's growth and development: There is no scientific evidence that me

Determine the functions of human body system, Determine the function of hum...

Determine the function of human body system Human body has different systems to perform different functions. A system consists of organs to perform a particular set of function

Chest x-ray, Increase in cardio-thoracic ratio is a relatively specific ind...

Increase in cardio-thoracic ratio is a relatively specific indicator of left ventricular end-diastolic volume. Left atrial enlargement is seen as double density shadow, lifting up

Kingdom, how many kingdom and phylum are there?

how many kingdom and phylum are there?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd