cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Explain nutrient and storage proteins, Nutrient and Storage proteins Th...

Nutrient and Storage proteins These proteins store amino acids. Ovalbumin is the protein of egg white, used as an amino acid source for the developing embryo. Casein, the prote

Coccidioidomycosis, Coccidioidomycosis Coccidioidomycosis is a soil-...

Coccidioidomycosis Coccidioidomycosis is a soil-borne infection of pet, domestic, wild animals and  man. The disease is caused by Coccidioides immitis, a dimorphic fungus wh

Mode of nutrition, explain the mode of nutrition in paramecium, euglena and...

explain the mode of nutrition in paramecium, euglena and hydra

Preformation theory - pre-mendelian theory, Preformatio n Theory (1632-172...

Preformatio n Theory (1632-1723) (i)         Scientist 'Malpighi Swammerdam' propounded the preformation theory. This theory holds that the sex cells (sperm and ova) had the m

Steps of initiation , The entire mechanism of protein synthesis i...

The entire mechanism of protein synthesis in eukaryotes is generally the same as in prokaryotes, with three phases explained as termination, elongation and initiation. Furthermore,

Determine the benedict test, Determine the Benedict's Test Glucose in u...

Determine the Benedict's Test Glucose in urine is detected by Benedict's method and the test is known as Benedict's test. This test is used to detect glucose in urine (Glycosur

Shapes - types of nursing units, Shapes: Nightingale Ward  In the 1...

Shapes: Nightingale Ward  In the 1940s,  this traditional nursing unit was based on  the long rectangle pavilion type ward and self-contained unit. This concept is establis

Explain about precautionary principle, Q. Explain about precautionary princ...

Q. Explain about precautionary principle? The ethical values of biodiversity highlights the intrinsic value of biodiversity for its own sake and it is independent of the varied

Explain the before abutment connection - implant failures, Before Abutment ...

Before Abutment Connection There can be wound dehiscence, and the presence of signs of infection such as swelling, fistula, pain prior to stage to surgery. It is important t

Relate fibro-osseous retention and osseointegration, Fibro-osseous Retenti...

Fibro-osseous Retention V/S Osseointegration Fibro-osseous retention is the tissue to implant contact interposition of the healthy, dense collagenous tissue between the implant

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd