cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Beta decay, Beta decay  is the type of radioactive decay in which the radio...

Beta decay  is the type of radioactive decay in which the radioisotope emits a little negatively-charged and fast-moving particle from its nucleus. A beta particle is almost simila

Major classes into which the phylum is divided, Q What are the major classe...

Q What are the major classes into which the phylum is divided? What are the few examples of each and in which form (polyp or medusae) are they found? Coelenterates are divided

Status of dehydration, The first step should be to determine the status of ...

The first step should be to determine the status of dehydration. We have already talked about the mild, inoderate and severe dehydration. In mild to moderate cases fluid, electroly

How to identify the monomer or molecular components, Please help with the f...

Please help with the following: For each of the four macromolecules carbs, lipids, proteins and nucleic acids, please identify the monomer or molecular components and name the b

Therapeutic diets - qualitative methods, Therapeutic diets - Qualitative me...

Therapeutic diets - Qualitative methods Qualitative methods: This  is where  you  give  the individual choices,  clear guidelines, menu guidance and supporting  information  su

Logical order in which molecules-atoms are associated, Q What is the logica...

Q What is the logical order in which the concepts of molecules, atoms, cells... up to biosphere are associated? Atoms form molecules that form cells that form tissues that form

Strategic and tactic control of parasitic diseases, S t r a tegic and t...

S t r a tegic and tactic control of parasitic diseases The reduced performance of the animals in terms of decreased milk yield, draught power, breeding performance, poor bo

Respiration, how energy enters and then flows through a biological system

how energy enters and then flows through a biological system

What are the physiological systems, What are the physiological systems know...

What are the physiological systems known as integrative systems? Why is this designation justified? The integrative systems are the nervous system and the endocrine system. The

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd