cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

What are the main cellular features of fungi, What are the main cellular fe...

What are the main cellular features of fungi? There are unicellular and pluricellular fungi. All fungi are eukaryotes and heterotrophs. Fungi have cells with cell wall made

How is the circulatory system of reptiles characterized, How is the circula...

How is the circulatory system of reptiles characterized? What is the basic difference among the reptile and the amphibian heart? The circulatory system of beings of the class R

Explain the requirements of special stains, Explain the requirements of Spe...

Explain the requirements of Special Stains? Special stains are required to make spores clearly visible. Different techniques like negative staining, dorner staining method, sch

What is the major constituent of the cell wall of bacteria, Q. According to...

Q. According to their morphology how bacteria are classified? Bacteria present different morphological patterns, a bacterium can be classified into bacillus, coccus, spirochete

Symptoms of aortic regurgitation, Q. Symptoms of aortic regurgitation? ...

Q. Symptoms of aortic regurgitation? Patients with chronic severe AR may be asymptomatic for many years and it may be picked up on routine examination. Some patients may remain

What occurs to transcription at the lac operon, What happens to transcripti...

What happens to transcription at the lac operon when lactose is absent and why? What happens to transcription at the lac operon when lactose is present and why? What happens

Explain the various types of protein structure, Explain the various types o...

Explain the various types of Protein Structure? Protein Structure :  The structure of proteins can be examined at four levels of increasing complexity, with the primary struc

Explain complete and incomplete digestive system , Normal 0 fal...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Explain the term active transport, Explain the term active transport? ...

Explain the term active transport? Active Transport :  At intervals, protein assemblies involved in selective, or active transport of materials are inserted into the cell mem

What are the three main types of trophic pyramids, What are the three main ...

What are the three main types of trophic pyramids studied in Ecology? The three types of trophic pyramids studied in Ecology are the numeric pyramid, the biomass pyramid and th

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd