cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Explain about extra low interstitial, Explain about Extra low Interstitial ...

Explain about Extra low Interstitial Extra low Interstitial (ELI) contains low levels of oxygen dissolved in interstitial sites in the metal. Lower amounts of oxygen and iron i

Management of symptomatic patients, Q. Management of Symptomatic Patients ...

Q. Management of Symptomatic Patients All symptomatic patients with severe aortic stenosis or even moderately severe aortic stenosis should undergo aortic valve replacement an

What proportion of children with down syndrome found, What proportion of ch...

What proportion of children with Down syndrome do you expect when women with down syndrome have children with men who have 46 chromosomes? Justify answer

Define role in protein metabolism and growth, Define Role in protein metabo...

Define Role in protein metabolism and growth? Severe vitamin A deficiency results in abnormal RNA metabolism and protein synthesis and hence interferes with growth. Hence vitam

What are the important organic molecules for living being, What are the mos...

What are the most important organic molecules for living beings? There are many kinds of organic molecules that are significant for the living beings. Especially vital are amin

Insulation by fur and feathers, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Carditis, It has been shown by prospective studies that Rheumatic heart dis...

It has been shown by prospective studies that Rheumatic heart disease (RHD) is linked to the occurrence of carditis during the first episode of ARF. If the first episode is accompa

Energy demand for active or moderately active lifestyles, Energy demand for...

Energy demand for Active or moderately active lifestyles? These people have occupations that are not strenuous in terms of energy demands, but involve more energy expenditure t

Reducing cost of accident, Reducing Cost of Accident One clear way to ...

Reducing Cost of Accident One clear way to control the cost of accident would be to follow all the rules of book for safety. All the methods of safety which were described ear

Why can the genetic code be qualified as a degenerate code, Why can the gen...

Why can the genetic code be qualified as a "degenerate code"? The genetic code is a degenerate code because there are amino acids codified by more than one type of codon. It is

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd