cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Avian (fowl cholera), Avian (fowl cholera) Fowl cholera is a contagious...

Avian (fowl cholera) Fowl cholera is a contagious septicaemic disease of almost all classes of fowl. The causal agent is Pasteurella multocida.Serotypes A:1 and A:3 are usually

What are homologous chromosomes, What are homologous chromosomes? Which are...

What are homologous chromosomes? Which are the human cells that do not have homologous chromosomes? Chromosomes have genes (genetic information in the form of nucleotide sequen

How do the golgi apparatus act, Q. How do the Golgi apparatus act and the r...

Q. How do the Golgi apparatus act and the rough endoplasmic reticulum in the production and releasing of proteins? The rough endoplasmic reticulum has in its outer membrane man

Show the circulatory system present in arthropods, Q. What is the kind of c...

Q. What is the kind of circulatory system present in arthropods? Do these animals have respiratory pigments and heart? In arthropods the respiratory system is open (lacunar). B

Principles of homeostasis with negative feedback, The principle of homeosta...

The principle of homeostasis is controlling the heating system with a simple thermostat in a house. These components are essentials thermometer, source of heat turning it on or off

Specific organization, Specific Organization The body of all living th...

Specific Organization The body of all living things, no matter how different in size or appearance, is composed of cells. Viruses are of course an exception. The study of cell

How sugar play a major role in preservation, How sugar play a major role in...

How sugar play a major role in preservation? In so many products, sugars play a major role in preservation. The addition of monosaccharides, like glucose or fructose, to jams a

From which floral structure do fruits come, From which floral structure do ...

From which floral structure do fruits come? Fruits are customized ovaries of the flowers.

What is dengue, What is dengue? Dengue, or dengue fever, is an epidemic...

What is dengue? Dengue, or dengue fever, is an epidemic disease in some countries (for instance, in Brazil), and its most dangerous form is hemorrhagic dengue. It is caused by

Endosperm of gymnosperms and the endosperm of angiosperms, How dissimilar a...

How dissimilar are the endosperm of gymnosperms and the endosperm of angiosperms? In the gymnosperms the endosperm is haploid (n), it is called as primary endosperm. In the ang

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd