cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Silviculture, Silviculture: It concerned with the cultivation of timber yi...

Silviculture: It concerned with the cultivation of timber yielding plants. Silviculture is the way of controlling the growth, establishment, composition, health or quality of fore

Microbes, what microbe is the one that is shaped like a ball with spikes co...

what microbe is the one that is shaped like a ball with spikes comming out

Human biology , Your final assignment is to write an (~2000 words) on any d...

Your final assignment is to write an (~2000 words) on any drug that is currently approved by the FDA, describing how the drug works based on the mechanisms of human biology describ

Blood flow, if i start in the superior mesenteric vain where do i have to g...

if i start in the superior mesenteric vain where do i have to go through to get to the left anterior circumflex humeral artery. by the way of the hepatic portal vein and the heart

Nursing process - bronchial asthma, Nursing Process Assessment ...

Nursing Process Assessment History, precipitating factors, current medication, medication used to relieve asthma symptoms. Any recent changes in medication regimen.

What is risk, What is Risk  Risk  :  A function of the probability o...

What is Risk  Risk  :  A function of the probability of an adverse effect and  the magnitude of that  effect,  consequential to hazard(s)  in food.

Which chamber does the blood go after leaving left atrium, Q. To which hear...

Q. To which heart chamber does the blood go after leaving the left atrium? What is the valve that separates these compartments? The valve between the left atrium and the left v

Spiracular control - respiration, Spiracular Control - Respiration ...

Spiracular Control - Respiration Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Determine net atp production after complete oxidation, How do you determine...

How do you determine net atp production after complete oxidation to CO2 and H2O using mitochondrial?-oxidation, the TCA cycle, the mitochondrial electron transport chain and oxidat

Brefily describe diffusion, Brefily describe diffusion ? Diffusion :  ...

Brefily describe diffusion ? Diffusion :  Diffusion is the process in which there is a net movement of particles of a substance from an area of higher concentration to an are

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd