cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Define glycolysis, Define glycolysis Glycolysis or the citric acid cy...

Define glycolysis Glycolysis or the citric acid cycle in which the direction of the reactions is well defined, the  interconversion reactions of the HMP pathway can function

Precaution for estimate the cholesterol using zlatkis method, Define Precau...

Define Precautions for Estimation of Cholesterol Using Zlatkis Method 1. Use aldehyde free glacial acetic acid. 2. Use only glacial acetic for making up and adjusting the vo

Explain the term- latent squint, Explain the term- Latent Squint (Anisophor...

Explain the term- Latent Squint (Anisophoria or Heterophoria) This eye condition occurs when the balance of extra-ocular muscles is altered. There is a tendency of the eye to

What is the typical biological function of the tissues, What is the typical...

What is the typical biological function of the connective tissues? How is this function associated to the main features of its cells? The typical function of the connective tis

Show subkingdoms into which the plant kingdom is divided, Q. What are the s...

Q. What are the subkingdoms into which the plant kingdom is divided? The kingdom Plantae is divided into two big subkingdoms: the tracheophytes and the bryophytes (gymnosperms,

Duck septicemia (duck pasteurellosis/new duck disease), D uck septicemia (...

D uck septicemia (duck pasteurellosis/new duck disease) The disease is caused by Pasteruella anatipestifer (Syn. Riemerella anatipestifer ) that mostly affects growing duck

Difference between adrenergic and cholinergic fibre, Difference between Adr...

Difference between Adrenergic and Cholinergic Fibre- S.NO .   1. ADRENERGI C FIBRE   Release nor adrenaline at their CHOLINE

What are the main classes into which the phylum is divided, What are the ma...

What are the main classes into which the phylum is divided? What are some examples of each and in which form (polyp or medusae) are they found? Coelenterates are separated into

Function of amino acids, FUNCTIO N OF AMINO ACIDS Proteins are poly...

FUNCTIO N OF AMINO ACIDS Proteins are polymers of amino acids. Glycine form porphyrin nucleus in chlorophyll and heme (= haem) proteins like haemoglobin and cytochrome

Types of fertilization, TYPES OF FERTILIZATION - On the basis of site o...

TYPES OF FERTILIZATION - On the basis of site of fertilization, it is of two types - 1. External fertilization        2. Internal fertilization 1 .       EXTERNAL FERTILIZ

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd