cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Bee and silkworm diseases, BEE DISEASES - Suffer from nosema diseases...

BEE DISEASES - Suffer from nosema diseases caused by Nosema apis. SILK WORM DISEASES - Pebrine disease caused by Nosema.

Medtronic hancock-biological valves, Medtronic Hancock (Standard Model) :  ...

Medtronic Hancock (Standard Model) :  These are gluteraldehyde preserved xenograft aortic valve, which are mounted. The modified orifice version (M-0) is one where the right

Define transcription phase of gene expression process, Define Transcription...

Define Transcription Phase of Gene Expression Process? Transcription is the enzyme synthesis of RNA on a DNA template. This is the first stage in the overall process of gene ex

Ornithophily - cross-pollination, Ornithophily - Cross-pollination In ...

Ornithophily - Cross-pollination In tropical areas, the birds dominate over insects as important pollinators. The most common among them are humming-birds, sun-birds and honey

Adult circulation and the human fetal circulation, Q. Concerning the mixtur...

Q. Concerning the mixture of arterial with venous blood what is the difference between the adult circulation and the human fetal circulation? In the human fetal circulation the

Assets and capital stock accounts, Q. Assets and capital stock accounts? ...

Q. Assets and capital stock accounts? At the present extend the liabilities, assets and capital stock accounts in the Adjusted Trial Balance columns to the Balance Sheet column

Animal tissue culture , Animal Tissue Culture: The term tissue culture ...

Animal Tissue Culture: The term tissue culture refers to the culture of all organs, tissue fragments and dispersed cells on a suitable nutrient medium. It may be divided into

What is vacuole , What is Vacuole ? Another structure found only in pla...

What is Vacuole ? Another structure found only in plant cells is the large central vacuole. The vacuole stores enzymes and waste products, in addition to providing the turgor p

Can we make artificial cells, Q. Can we make artificial cells? Research...

Q. Can we make artificial cells? Research in progress at the "National Aeronautical and Space Administration (NASA)" is focused on artificial cells as a means to deliver medici

Mention the role o ribosome in peptide, Mention the role o ribosome's in pe...

Mention the role o ribosome's in peptide -bond formation .How does ATP facilitate it? a) Of springs derived by asexual reproduction are known as clones. Justify giving two R

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd