cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Explain autosomal recessive disease, The frequency of an autosomal recessiv...

The frequency of an autosomal recessive disease in a population is 1 in a million. An unaffected person who had a sibling affected with this disease marries a lady from the pop

Is acetylcholine found in brain, Q. Is Acetylcholine found in brain? Ace...

Q. Is Acetylcholine found in brain? Acetylcholine is found in many parts of the brain having particularly high concentrations in the cerebral cortex, limbic system, basal, foreb

Sterigmatocystin, These mycotoxins are structurally and biologically relat...

These mycotoxins are structurally and biologically related to the aflatoxins, and like the latter, they cause hepatocarcinogenic activity in animals. Among the organisms that prod

How different are birds and reptiles, Q. How different are birds and reptil...

Q. How different are birds and reptiles concerning the maintenance of body temperature? Are birds rare in polar regions? Reptiles are heterothermic that is they do not control

Define some precautions for estimation of amount of bacteria, Define some P...

Define some Precautions for Estimation of the Amount of Bacteria? 1. Dilution should be made carefully. 2. Use fresh sterile pipette for making each dilution. 3. Aseptic

Glycolysis, steps involved in glycolysis

steps involved in glycolysis

Define can we improve the nutritive value of protein, Define Can we improve...

Define Can we improve the nutritive value of protein? Yes, as discussed above, the nutritive value of a protein can be improved in two ways: (1) by mutual supplementation, that

What is the function of the umbilical cord, What is the function of the umb...

What is the function of the umbilical cord? The umbilical cord is a set of blood vessels that connects the fetus with the placenta. In the fetus one extremity of the cord inse

Explain different amino acids found in proteins, How many different protein...

How many different proteins, each composed of 8 amino acids, can be constructed using the 20 different amino acids found in proteins?

Name two food additives needed to keep food wholesome, a) Name two food ad...

a) Name two food additives needed to keep food wholesome, and say what they do. b) Name two food additives (or types of additive) which are not necessary for keeping fo

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd