cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

What are homologous chromosomes, What are homologous chromosomes? Which are...

What are homologous chromosomes? Which are the human cells that do not have homologous chromosomes? Chromosomes have genes (genetic information in the form of nucleotide sequen

Classify biomaterials and list some examples, Q. Classify biomaterials and ...

Q. Classify biomaterials and list some examples? Biologic classification is based on tissue response and systemic toxicity effects of the implant and is divided into three clas

Define specialized nutritional formulations for chd, Define Specialized Nut...

Define Specialized Nutritional Formulations for CHD? Achieving calorie and protein requirements with restricted fluid intake, you would realize, is a formidable challenge. Hig

Show the embryological evidence, Q. Show the Embryological Evidence? Em...

Q. Show the Embryological Evidence? Embryology is the study of the successive stages of sporogencsis , gametogenesis and the growth and development of the embryo. The number o

Poisoning, Poisoning: Poisoning  is a common medical emergency  in chi...

Poisoning: Poisoning  is a common medical emergency  in childhood. In children under 5 years of  age essentially all poisoning are accidental. Nearly 75 per  cent  of  all poi

Life processes, why the small intestine is longer in herbivores as comparis...

why the small intestine is longer in herbivores as comparison to carnivores animals

Explain about the bifidogenic effect, Explain about the Bifidogenic Effect?...

Explain about the Bifidogenic Effect? Fructans selectively stimulate the growth of Bifidobacteria and Lactobacilli, while decreasing concentrations of E.coli, Clostridia and ba

Tolrance, geographical range graph on minimum and maximum range of toleranc...

geographical range graph on minimum and maximum range of tolerance

Botany, Suggest a mechanism that would allow a plant to grow better if it h...

Suggest a mechanism that would allow a plant to grow better if it had intermorph neighbors than if it had intramorph neighbors. Think about the degree of genetic similarity between

Tumours of kidney - wilm''s tomour, Tumours of kidney - Wilm's Tomour ...

Tumours of kidney - Wilm's Tomour So  far you have learnt about the disorders in glomerular dysfunctioning, now you will learn about the tumours in kidney the wilm's  tumour.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd