cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

What are the differences between mitosis and meiosis, Concerning their fina...

Concerning their final products (daughter cells and their ploidies) what are the differences between mitosis and meiosis? In mitosis one cell, for example, with 2n chromosomes,

Entomophily - cross-pollination, Entomophily - Cross-pollination It in...

Entomophily - Cross-pollination It involves insects to carry the pollen to achieve pollination. Salvia exhibits a specialised 'tumapipe' floral mechanism that signifies classi

How does self infection by tapeworms occur, How does self infection by tape...

How does self infection by tapeworms occur? Taeniasis patients might be develop the most severe form of the worm infection, cysticercosis, because their feces have eggs and pre

Apical dominance, Apical Dominance Apical dominance is a correlative p...

Apical Dominance Apical dominance is a correlative phenomenon in the developmental biology of plants. Branching of the main shoot into lateral branches is one of the main char

Define beneficial effects of functional foods, Define beneficial effects of...

Define beneficial effects of functional foods? Several beneficial effects of functional foods have been reported, which include effects such as antioxidant, anticarcinogenic, b

Sunshine recorder, Sunshine recorder Sunshine recorder (Figure shown be...

Sunshine recorder Sunshine recorder (Figure shown below) measures the duration of sunshine. The recorder consists essentially of a glass sphere of about 10 cm in diameter mount

How can neuroscience help to find neurological disorders, How can "basic" ...

How can "basic" neuroscience research help to find cures for neurological disorders? A better understanding of the brain at each level - molecules, cells and neural systems -is

What could have caused the discrepancy, If the class's value did not match ...

If the class's value did not match the expected value of water, let's assume everyone was actually correctly using the devices and there was no "user error." What could have caused

Test for starch, have any picture this expiriment at record?

have any picture this expiriment at record?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd