cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Hmp shunt in erythrocytes, HMP shunt in erythrocytes HMP shunt in eryth...

HMP shunt in erythrocytes HMP shunt in erythrocytes is of importance due to the generation of NADPH, which maintains the glutathione (G-SH)  in  the reduced  state by  glutathi

What are the processes that autotrophic beings, What are the processes that...

What are the processes that autotrophic beings use to produce organic material from inorganic substances? The Autotrophic beings make organic material by photosynthesis or by c

Is this the same thing as tonicity, These labs discuss the principle of osm...

These labs discuss the principle of osmolarity, which is defined as the total number of solute particles in 1 L of solution. Is this the same thing as tonicity? Explain your answer

Why networking in hospitals, WHY NETWORKING IN HOSPITALS Sharing of dat...

WHY NETWORKING IN HOSPITALS Sharing of data is essential, especially in any kind of hospital setting. For staff and doctors of a hospital, if they have details of patients rea

Pisces fish, description on characteristics of pisces fish

description on characteristics of pisces fish

Biochemical production, Plants are the source of a large variety of bioche...

Plants are the source of a large variety of biochemicals which are metabolites of both primary and secondary metabolism. But secondary metabolites are of much greater interest s

Transferring the patient form icu to ward, Transferring the Patient form IC...

Transferring the Patient form ICU to Ward The patient is made to ambulate in ICU before shifting to ward . vitals are stabilized. The ward nurse is explained the pa

collection of solid waste, There are three methods of collections: 1.  ...

There are three methods of collections: 1.      Kerbside collection: In this collection system the refuse is brought in containers and placed on the footway, from where it is

What is aprecise diagnosis, Q. What is Aprecise diagnosis? Aprecise dia...

Q. What is Aprecise diagnosis? Aprecise diagnosis is necessary to establish the mode of therapy. Patients with initial blood phenylketonuria level of 121 m moll1 (> 2 mg/dl) sh

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd