cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Define health and feeding problems among elderly, Define Health and Feeding...

Define Health and Feeding Problems among Elderly? Malnutrition, both obesity and under nutrition, are common problems linked with elderly.  Some of the common disorders such as

Cytology, who is the first person who saw the cell

who is the first person who saw the cell

Define the soil genesis, SOIL GENESIS Soil a product of evolution, is c...

SOIL GENESIS Soil a product of evolution, is constantly changing as the landscape changes. It is a product of the interaction of five factors: parent material, climate, organis

The genetic code within a dna molecule, How is a genetic trait determined b...

How is a genetic trait determined by the genetic code within a DNA molecule? A DNA molecule has 4 dissimilar bases, either CGTA. Any specific combination of these things forms

What is class crinoidea, What is Class Crinoidea? This Class includes t...

What is Class Crinoidea? This Class includes the sea lilies and the feather stars. What makes them different from the other Echinoderms is the placement of their mouth and anus

Unconventional oilseed cakes/meal, Unconventional oilseed cakes/meal ...

Unconventional oilseed cakes/meal Deoiled sal (Shorea robusta) seed meal: It is a byproduct obtained after the extraction of oil from sal seeds. It is found to be high in NF

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd