cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Define homocysteinemia, Possible mechanisms of increased risk are that hype...

Possible mechanisms of increased risk are that hyperhomocystinemia may impair release of nitric oxide form endothelial cells, stimulate proferation of atherogenic smooth muscle cel

What is the non-paralytic squnit, Concomitant or Non-Paralytic Squnit ...

Concomitant or Non-Paralytic Squnit This type of squint usually occurs when there is an obstacle in the sensory pathway of binocular reflex such as refractive errors, opacities

Estimate the molar mass of protein, Estimate the molar mass of protein? ...

Estimate the molar mass of protein? An aqueous solution of the protein bovine serum albumin, containing 2:00 *10 -2 g of protein per cubic centimeter, has an osmotic pressure

Find the magnitude and direction of the net electric force, Three point cha...

Three point charges are located at the corners of an equilateral triangle as in the figure below. Find the magnitude and direction of the net electric force on the 1.40 µC charge.

How do mineral salts participate in enzymatic activity, How do mineral salt...

How do mineral salts participate in enzymatic activity? Many mineral salts are cofactors of enzymes. i.e., they are substances without which enzymes do not work.

Genome, Genome is the whole compliment of genetic material in the form of ...

Genome is the whole compliment of genetic material in the form of the permanently maintained DNA for the particular given organism. Its size is usually given as its total number o

What is nidation, What is nidation? In which phase of the menstrual cycle d...

What is nidation? In which phase of the menstrual cycle does nidation occur? Nidation is the implantantion of the embryo in the uterus. Nidation happens around the 7th day afte

Define effect of vitamins (a, Define the effect of Vitamins (A, D, K and B-...

Define the effect of Vitamins (A, D, K and B-Complex) on athletes? Vitamins A, D and K have been found to have no ergogenic effects. Ingesting large doses of these vitamins hav

Increase in the divcat conductance of neuron b, Consider Neuron B in the fr...

Consider Neuron B in the frog central nervous system whose plasma membrane has a previously unknown channel that is selectively conductive to a newly discovered divalent cation nam

Age-sex distribution, Age-Sex Distribution While studying exponential...

Age-Sex Distribution While studying exponential growth we need to know more than just the birth and death rates because these rates vary with age and sex. Age structure in a

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd