cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Detritus food chains, Detritus Food Chains Detritus food chains begin ...

Detritus Food Chains Detritus food chains begin with dead organic matter which is an important source of energy. A large amount of organic matter is contributed by the death o

Illustrate photochemical phase, Q. Which are the subproducts of the photoch...

Q. Which are the subproducts of the photochemical phase that are essential for the chemical stage of photosynthesis? The chemical stage of photosynthesis depends on ATP and NAD

Define who classification for assessment of vitamin a status, Define WHO cl...

Define WHO classification for assessment of vitamin A status? In addition, to the above mentioned manifestations, thickening of the hair follicles (follicular hyperkeratosis) i

Nutrion, what are the different modes of nutrition

what are the different modes of nutrition

What are some examples of secretory cells, What are some examples of secret...

What are some examples of secretory cells? Endocrine and exocrine pancreatic cells, thyroid and parathyroid endocrine cells, adenohypophysis, adrenal and pineal endocrine cell

Non-viral vectors, Non-viral vectors   Viral vectors are highly effici...

Non-viral vectors   Viral vectors are highly efficient but when it comes to large scale production at the commercial level, non-viral serve as a better choice. These methods p

How is the circulatory system of birds characterized, How is the circulator...

How is the circulatory system of birds characterized? Birds, as every vertebrate, have a closed circulatory system. The heart is same to the mammalian heart, having four chambe

Ice age, Ice age is an interval of geologic time between 2 million and 10,...

Ice age is an interval of geologic time between 2 million and 10,000 years ago during that period the northern hemisphere experienced several episodes of continental glacial advan

What are the valves of the venous system, What are the valves of the venous...

What are the valves of the venous system? What is their function? The valves of the venous system are structures within the veins that permit blood to flow only in the normal w

Pollutions, what is detergent pollution and it''s effects and causes

what is detergent pollution and it''s effects and causes

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd