cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Hospitalization, Hospitalization Hospitalization is stressful experien...

Hospitalization Hospitalization is stressful experience  for children,  adolescents  and their families.  It is an interruption  of the child's active cycle of growth and deve

Interdependance, Give an example of how two organisms are interdependent.

Give an example of how two organisms are interdependent.

Self help group - psychoactive substance use disorders, Self Help Group: ...

Self Help Group: The first and perhaps the most influential of substance abuse rehabilitation is Self Help Group. The self help programme was founded in 1935 by two men, Dr. B

Explain transition period from renaissance to modern period, Explain Transi...

Explain Transition Period from Renaissance to Modern Period? The transition period from the Renaissance to the Modern period produced many notable workers and much literature.

Nonshivering thermogenesis, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Clinical evaluation in the continuing care cycle, Clinical Evaluation in ma...

Clinical Evaluation in maintenance in the Continuing Care Cycle Examination of gingival contours, along with mechanical evaluation of the prosthesis, implants, and implant comp

Describe amoebae - paramecia and trichomonas, Q How do amoebae, paramecia a...

Q How do amoebae, paramecia and trichomonas respectively move? Amoebae move by amoeboid movements small invaginations and projections of their plasma membrane (pseudopods) that

Precaution for estimate the cholesterol using zlatkis method, Define Precau...

Define Precautions for Estimation of Cholesterol Using Zlatkis Method 1. Use aldehyde free glacial acetic acid. 2. Use only glacial acetic for making up and adjusting the vo

Microorganisms on basis of oxygen requirement for growth, Q. Microorganisms...

Q. Microorganisms on basis of oxygen requirement for growth? On basis of oxygen requirement for growth: - Obligate Aerobes: Require oxygen for growth and multiplication e.g

Can you explain the process of twins generation, Q. What are twins? Genetic...

Q. What are twins? Genetically what are the two types of twins that can be generated? Twins are at the same time generated (within the mother's uterus) offspring. Twins classif

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd