cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Additional substances does a plant need to make amino acid, a) What additio...

a) What additional substances does a plant need to make amino acids and proteins from glucose? b) Where do these substances come from?   (a) To make amino acids

What do you understand by polyp?, What do you understand by Polyp? The ...

What do you understand by Polyp? The sessile, asexual stage in the cnidarian life cycle. In some species they are independent organisms; in others, they form colonies where som

Hypothalamus, Hypothalamus functions are generally aimed at maintaining A ...

Hypothalamus functions are generally aimed at maintaining A region in the brain beneath the thalamus; consists of number of aggregations of nerve cells and controls a variety of a

What is metabolic disorders, Q. What is metabolic disorders? In these m...

Q. What is metabolic disorders? In these metabolic disorders, since there is an inability to metabolize a specific amino acid the basis of dietary management is to restrict the

How substrate concentration affect initial rate of enzyme, How does substra...

How does substrate concentration affect the initial rate of an enzyme- catalyzed reaction?

Define aerobic exercise or aerobic energy system, Define Aerobic Exercise o...

Define Aerobic Exercise or Aerobic Energy System? To produce necessary energy, the body uses an aerobic pathway and an anaerobic pathway. Exercise that relies heavily on oxygen

What occurs to transcription at the lac operon, What happens to transcripti...

What happens to transcription at the lac operon when lactose is absent and why? What happens to transcription at the lac operon when lactose is present and why? What happens

Phases of karyokinesis, Phases of Karyokinesis: Karyokinesis can furth...

Phases of Karyokinesis: Karyokinesis can further be divided into prophase, metaphase, anaphase and telophase. 1-Prophase: (pro= before) Chromatin: During Interphase (non

Difference between lamarckism and neo-lamarckism, DIFFERENC E BETWEEN LAMA...

DIFFERENC E BETWEEN LAMARCKISM AND NEO-LAMARCKISM - S.No Lamarckism Neo-Lamarckism 1. It refers of the original

Getting ready to grow bacteria, Getting ready to grow bacteria Secure t...

Getting ready to grow bacteria Secure two or three dozen shallow glass dishes. The glass coasters used to stay bed castors from marring floors will do. Cut 5 cm squares of wind

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd