cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Mechanism of phloem transport, Mechanism of Phloem Transport The effic...

Mechanism of Phloem Transport The efficiency and magnitude of translocation of food material are evident from the annual yields of various crops and fruits. Now, the question

Waste management in other species, W a s t e Management in other specie...

W a s t e Management in other species Solid and pellet nature of sheep and goat faecal matter make it easier to handle. This manure can easily be swept off the floors and d

Explain third mitotic period, Q. What are the major events of the third mit...

Q. What are the major events of the third mitotic period? The third mitotic period is anaphase. In anaphase the following events occur breaking and duplication of centromeres w

Common symptoms linked to lactose intolerance, Q. Common symptoms linked to...

Q. Common symptoms linked to lactose intolerance? Common symptoms linked to lactose intolerance include: 1. Anorexia and nausea. 2. Intestinal distension 3. Abdominal

Describe the important factors of hand washing, Describe the important fact...

Describe the important factors of Hand washing Hand washing is an important practice to follow before doing any procedure for the patient. The infection can be transferred

Mathematical and statistical challenges, Progress on these biological issue...

Progress on these biological issues needs both that more mathematics and statistics be brought to bear on them and that new mathematical and statistical tools be prepared. All the

A patient was complaining of frequent urination, A patient was complaining ...

A patient was complaining of frequent urination, excessive thirst and dehydration. His fasting glucose level was found to be normal. Name the disease and its cause. Describe

Endocrine glands - placenta, PLACEN T A - Placenta is the intimate...

PLACEN T A - Placenta is the intimate connection between the foehls and the uterine wall of the mother to exchange the materials. Placenta is a tempormy endocrine gland

Agro industrial-proteins, Proteins Protease inhibitors: Protease inh...

Proteins Protease inhibitors: Protease inhibitors are protein-based substances widely distributed within the plant kingdom, including the seeds of most cultivated legumes wh

Define recommended dietary allowance for pyridoxine (rda), Define Recommend...

Define Recommended Dietary Allowance for pyridoxine (RDA)? Average requirements for pyridoxine vary with age, sex and physiological conditions such as protein status, pregnan

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd