cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Immediate post-operative care to a cardiac patient, Immediate Post-operativ...

Immediate Post-operative Care The patient is accompanied to the ICU by a surgeon, anesthetist and the nurse who assisted for the surgery with portable ventilator and ECG

State the types of brain trauma, State the types of brain trauma? There...

State the types of brain trauma? There are two main types of brain trauma a) open head injury and b) closed head injury

What is crystallization of a virus, What is crystallization of a virus? Wha...

What is crystallization of a virus? What is the importance of this process? Crystallization is the method of transformation of viral components into organized solid particles.

Proteins - digestion process, Proteins are very essential macromolecules as...

Proteins are very essential macromolecules as they have a range of many important functions like: • Required for building and repair of body tissues (including muscle) • Enzy

What is end-to-end anastomosis subclavian flap aortoplasty, What is End-to-...

What is End-to-End Anastomosis with Subclavian Flap Aortoplasty ? The two operations can be combined as a single procedure. Medially end-to-end anastomosis is done between the

Define corneal scar - micronutrient deficiencies, Define Corneal Scar - Mic...

Define Corneal Scar - Micronutrient Deficiencies? The corneal ulcer, on healing, leaves a white scar, which may vary in size depending upon the size of the ulcer. When the scar

Following statement true for prokaryotic or eukaryotic, Following statement...

Following statement true for prokaryotic or eukaryotic? They use deoxyribonucleic acid as their major information storage molecule

Glanders, Glanders The glanders is caused by Burkholderia mallei (previous...

Glanders The glanders is caused by Burkholderia mallei (previously known as Malleomyces mallei) and it is a serious contagious disease of equines. Infected equidae are the reservo

Can we make artificial cells, Q. Can we make artificial cells? Research...

Q. Can we make artificial cells? Research in progress at the "National Aeronautical and Space Administration (NASA)" is focused on artificial cells as a means to deliver medici

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd