cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Are birds rare in polar regions, How different are reptiles and birds conce...

How different are reptiles and birds concerning the maintenance of body temperature? Are birds rare in polar regions? Reptiles are heterothermic, i.e., they do not control thei

Distinguish, distinguish between striated and cardic muscles

distinguish between striated and cardic muscles

Definition of consciousness, Q. Definition of Consciousness? Consciousn...

Q. Definition of Consciousness? Consciousness is a phenomenon that is shared by nearly all people. It cannot be directly seen or touched. Yet it is real enough to most people.

#title, Feeding mechanism in holozoic organisms

Feeding mechanism in holozoic organisms

How is plant tissues classified according to their functions, How is plant ...

How is plant tissues classified according to their functions? Plant tissues are separated into growth (embryonic) supporting, tissues, filling and photosynthetic tissues (groun

What is the function of the testicles, Q. Concerning reproduction what is t...

Q. Concerning reproduction what is the function of the testicles? The testicles are the male gonad that is the organs where the production of gametes takes place. In human bein

How does sexual reproduction occur in bacteria, How does sexual reproductio...

How does sexual reproduction occur in bacteria? How different are the modalities of bacterial sexual reproduction? Sexual reproduction happens when bacteria incorporate genetic

What is implant success, Implant success 1) An individual, unattached i...

Implant success 1) An individual, unattached implant is immobile when tested clinically. 2) A radiograph that does not demonstrate any evidence of perimplant radiolucency.

True about microbial metabolism, which of the following is most likely to b...

which of the following is most likely to be true of microbial metabolism? 1)Acetyl-CoA is converted directly into 2 ATP 2)The energy from 2 ATP is necessary to drive the electron t

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd