cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Surgery for coronary artery disease, SURGERY FOR CORONARY ARTERY DISEASE : ...

SURGERY FOR CORONARY ARTERY DISEASE : Stenotic coronary artery disease (CAD) is caused by the thickening and narrowing of the coronary arteries (Atherosclerosis). Initially it cau

Pulsatile perfusion-type of blood pump, Pulsatile Perfusion: Conventional...

Pulsatile Perfusion: Conventional pumps give continuous flow with very little pulsatile property. Pulsatile perfusion is likely to result in better post operative renal and cardi

Steps for elongation , a) The elongation level of translation in eukaryo...

a) The elongation level of translation in eukaryotes requires three elongation factors, eEF-IB, eEF-1A and eEF-2, that have similar functions to their prokaryotic counterparts EF-G

What a cell with a euploid number of chromosomes contains, A cell with a eu...

A cell with a euploid number of chromosomes contains: A.one half of a complete set of chromosomes B.several complete sets of chromosomes C.the correct number of chromosomes D.two c

Variety of locomotion, Variety of Locomotion A number of animals are s...

Variety of Locomotion A number of animals are sendentary, remaining attached to some substratum. These animals may move parts of their body but this does not result in the mov

Functional properties of cardiac muscle, Normal 0 false false...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Define calcium - the macronutrient, Define Calcium - The Macronutrient? ...

Define Calcium - The Macronutrient? Calcium is an important mineral found mainly in bone and teeth. It is widely distributed in food substances such as milk, cheese, egg yolk,

Advantages and disadvantages of organizational charts, Advantages and Disad...

Advantages and Disadvantages: of Organizational Charts Advantages  a)  Defining the Organizational Relationship Without a,chart, many people might view the organization as

The deficiency in hepatic glucose 6 phosphatase, Under fasting conditions, ...

Under fasting conditions, individuals with a deficiency in hepatic glucose 6 phosphatase cannot dephosphorylate glucose 6 phosphate generated from liver glycogenolysis. As a result

Define feeding options for premature and lbw babies, Define Feeding Options...

Define Feeding Options for Premature and LBW Babies? Different workers have tried different method and since all methods are successful, it is dependent on the individual infan

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd