cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Explain mid-diastolic and lafe diastolic pre systolic sound, Explain Mid-di...

Explain Mid-diastolic and Lafe Diastolic (Pre Systolic) Sounds ? Mid-diastolic sounds are S3 sounds and late diastolic (pre systolic) sounds are S3 sounds. Pre systolic Pacema

What is phylum annelida - segmented worms, What is Phylum Annelida - Segmen...

What is Phylum Annelida - Segmented Worms? Members of this Phylum are commonly known as the segmented worms. The body of a segmented worm is made of many individual ring-like s

What might be the value of the everglade restoration plan, What might be th...

What might be the value of the Everglade restoration plan? Everglades will protect endangered species, such as the Florida panther, and restore the water flow needed by many or

Write short note on cholesterol, Cholesterol, from stereos (solid) and the...

Cholesterol, from stereos (solid) and the Greek chole- (bile) followed by the chemical suffix -ol for an alcohol is an organic chemical substance classified as a waxy steroid of fa

1.notochord, general character and classification of platyhelminthes

general character and classification of platyhelminthes

Food and digestion, what is the pH inside the stomach?Why is there a need t...

what is the pH inside the stomach?Why is there a need to keep that pH level? How is it maintained?

Consider the evolution of metabolic pathways, Consider the evolution of met...

Consider the evolution of metabolic pathways. Some biologists believe that these pathways evolved from?

Amphibia, an assignment of amphibians

an assignment of amphibians

What are the main arguments that favor evolutionism, In the scientific comp...

In the scientific competition against fixism what are the main arguments that favor evolutionism? The major arguments in favor of evolutionism are: paleontological, from the st

All part., Wat type of animal is tick and weevil?

Wat type of animal is tick and weevil?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd