cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

History of psychiatric nursing, History of Psychiatric Nursing: In 187...

History of Psychiatric Nursing: In 1873, Linda Richards, developed better nursing care in psychiatric hospitals, and organized nursing services and educational programmes  in

What is total cavo pulmonary connections, What is Total Cavo Pulmonary Conn...

What is Total Cavo Pulmonary Connections (TCPC)-Lateral Tunnel Operation? Cardiopulinonary bypass is instituted by cannulation of ascending aorta, SVC innominate vein junction

Nature of neuropsychological tests, Nature of neuropsychological tests ...

Nature of neuropsychological tests Neuropsychological tests are aids in the neuropsychological examination. The tests calculates specified psychological processes including the

What is the life duration of the red blood cells, Q. On average what is the...

Q. On average what is the life duration of the red blood cells? Where are they destroyed? What is the destination of the heme groups after the destruction of hemoglobin molecules?

What is ammensalim, What is ammensalim? Ammensalism is the ecological i...

What is ammensalim? Ammensalism is the ecological interaction in which an individual harms another without obtaining advanatage. Ammensalism is an inharmonious (negative) ecolo

Acute complications of diabetes, Q. Show Acute Complications of Diabetes? ...

Q. Show Acute Complications of Diabetes? Acute complications of diabetes include: Hypoglycemia or low blood sugar: The most frequent cause of low blood sugar is poor timing

Explain surface properties of proteins, Explain Surface properties of prote...

Explain Surface properties of proteins The surface properties relates primarily to surface tension, a) Emulsification  and b) Foaming characteristics of proteins,

What are exocrine respective hormones and enzymes, What are the pancreatic ...

What are the pancreatic tissues involved respectively in the exocrine and endocrine secretions? What are their respective hormones and enzymes? The exocrine secretion of the pa

What are passive and active immunization, What are passive and active immun...

What are passive and active immunization? According to the duration of the protection how do these types of immunization differ? Active immunization is that in which an antigen

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd