cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Patient with diabetes mellitus, Educating a patient with diabetes mellitus....

Educating a patient with diabetes mellitus. At this point you have understood in detail about diabetes mellitus, its management and complications. You can appreciate now why it is

How do cells obtain energy for their functioning, How do cells obtain energ...

How do cells obtain energy for their functioning? Cells obtain energy for their metabolic reactions from the breaking of organic molecules with high energetic content. This ene

Standard titration - estimation of vitamin c in chillies, Define Standard t...

Define Standard titration - estimation of vitamin c in Chillies? Pipette 5 ml of standard ascorbic acid solution into a 100 ml conical flask. Fill the burette with the dye solu

Which types of mammal are included in the primate group, Which types of mam...

Which types of mammal are included in the Primate group?  The Primate group contains a) Lemurs, b) Monkeys, c) Apes and humans.

Fidelity - synthetic characters, Fidelity - Synthetic Characters Fidel...

Fidelity - Synthetic Characters Fidelity refers to the degree to which a species is restricted in its occurrence to a particular kind of community. The species with low fideli

What are some examples of biological activities, Q. What are some examples ...

Q. What are some examples of biological activities in which osmosis plays an significant role? Hemolysis destruction of red blood cells by entrance of water, the hydric regulat

What are the basic constituents of the cell membrane, The cell membrane is ...

The cell membrane is produced of lipids, proteins and carbohydrates. The membrane lipids are phospholipids, a particular type of lipid to which one extremity a phosphate group i

Explain gelation - function of proteins, Explain Gelation - Function of pro...

Explain Gelation - Function of proteins Gelation  refers to the process where denatured molecules aggregate to form an ordered protein network. Proteins  can form a well-order

Define intracellular fluid or the water within the cells, Define intracellu...

Define intracellular Fluid or the water within the cells? The intracellular Fluid or the water within the cells makes up about 40-4596 of the total body weight. Because the bod

Determine about the prokaryotes and eukaryote cell, Determine about the pr...

Determine about the prokaryotes and eukaryote cell The eukaryotic cells, on the other hand, have a true nucleus wherein the genetic material, DNA is enclosed within a well-def

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd