cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

What is exudative diarrhoea, Q. What is Exudative diarrhoea? Exudative ...

Q. What is Exudative diarrhoea? Exudative diarrhoea: It is associated with the mucosal damage leading to out pouring of mucus, blood and plasma proteins with a net accumulation

Splenic abscess, Splenic infarction is a common complication of left-sided ...

Splenic infarction is a common complication of left-sided IE (40 per cent of cases). Only 5 per cent of patients with splenic infarction will develop splenic abscess. This infectio

What are some examples of biological activities, What are some examples of ...

What are some examples of biological activities in which osmosis plays an important role? Hemolysis (destruction of red blood cells) by entrance of water, the hydric regulation

What is the heterotrophic hypothesis on the origin of life, What is the het...

What is the heterotrophic hypothesis on the origin of life? As per to the heterotrophic hypothesis the first living beings were very simple heterotrophic organisms that is not

How much dopamine do need add from a stock, A cat has just arrived to the v...

A cat has just arrived to the veterinary clinic with low blood pressure. The cat weighs 5 kg, and as the on call Vet you want to use a bag of saline to prepare a dopamine drip for

Basic principles of planning diets for diabetes, Q. Basic principles of pla...

Q. Basic principles of planning diets for diabetes? The above objectives can be met by adhering to some of the basic principles of planning diets which include the consideratio

What do you understand by abdomen, In organisms that have undergone tagmosi...

In organisms that have undergone tagmosisthe trunk often develops into two tagma, oneinvolved in locomotion and one that is notlocomotory. The tagma not involved in locomotionis re

Excretion, what is the excretory organ of agama lizard

what is the excretory organ of agama lizard

Hypoglycaemia unawareness, Hypoglycaemia Unawareness ? Some people ...

Hypoglycaemia Unawareness ? Some people with diabetes do not have early warning signs of low blood glucose, a condition called hypoglycemia unawareness. ? This conditio

What are the habits effect on implant, Habits: Any abnormal habits need...

Habits: Any abnormal habits needs to be identified. Following habits may have detrimental effect on the implant: 1. Parafunction -  Bruxism/ Clenching 2. Tongue Thrusting

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd