cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Population growth, Increase in population size is known as population growt...

Increase in population size is known as population growth. It depends upon number of persons added to the population and number of persons lost from the population. Addition in pop

Determine the theory of evolution, What is the problem that the theory of e...

What is the problem that the theory of evolution and its rival theories try to solve? The problem that the theory of evolution, or simply evolution, and its rival theories try

Preparation of patient for fasting blood sugar analysis, Preparation of Pat...

Preparation of Patient for Fasting Blood Sugar Analysis 1. Blood for fasting blood sugar (plasma glucose) analysis should be drawn after the individual has fasted overnight (8-

Excretion, living organisms and their excretory products

living organisms and their excretory products

Horse diseases-equine viral arteritis, Equine viral arteritis Equine v...

Equine viral arteritis Equine viral arteritis (EVA) is a highly contagious disease of equines and is caused by equine arteritis virus (EAV), a RNA virus belonging to the genus

What do you undersand by pollution, Q. What do you undersand by pollution? ...

Q. What do you undersand by pollution? The Pollution is the contamination of an ecosystem by factors that are harmful for the equilibrium of its abiotic or biotic constituents.

What are dendritic cells, Dendritic Cells a. Are the same as macrophages b....

Dendritic Cells a. Are the same as macrophages b. Bind and digest pathogen proteins c. Secrete immunoglobulin d. All of the above

Why are the salt and sugar used, Q. Why are the salt and sugar used in the ...

Q. Why are the salt and sugar used in the production of dried fruits and dried meat? Substances that sustain a highly hypertonic environment, like salt and sugar, are used in t

What are the steps in bacterial infection, It contain four steps. They are:...

It contain four steps. They are: Attachment to host Proliferation Invasion of host tissue Toxin-induced damage to host cell

Improvement of soil aeration-aggregate structure, Improvement of Soil Aerat...

Improvement of Soil Aeration Soil organisms greatly improve soil structure and facilitate aeration. Root decay leaves the soil riddled with channels, and the burrowing of worm

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd