cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Embryology in relation to taxonomy, Q. Embryology in relation to taxonomy? ...

Q. Embryology in relation to taxonomy? Embryological information has been used for taxonomic purposes at various levels of classification. You know of a very basic division of

Photosynthesis, Photosynthesis Photosynthesis - the process by which p...

Photosynthesis Photosynthesis - the process by which plants utilise energy from sunlight to convert carbon dioxide and water for the synthesis of sugar. This sugar can then be

Statistical investigation, Meaning of Statistical Investigation: An inv...

Meaning of Statistical Investigation: An investigation means a systematic search for the knowledge or the truth. In statistical investigation the search for the knowledge is do

Respiration, how does respiration in animals occur? what is respiration? wh...

how does respiration in animals occur? what is respiration? what are the common types of respiration?

What is diffusion, Diffusion is the spreading of substance molecules from a...

Diffusion is the spreading of substance molecules from a region where the substance is more concentrated to other region where it is less concentrated. For example, during the boil

Why do sharks need to agitate their body to swim, Q. How different are the ...

Q. How different are the swimming strategies in chondrichthyes and in osteichthyes? Why do sharks need to agitate their body to swim while bony fishes do not? Bony fishes have

Define the importance of iodine in human, Define the importance of Iodine i...

Define the importance of Iodine in human? Iodine derives the nutritional importance as a constituent of thyroid hormones, 3,5,3',5' tetraiodo-thyronine (thyroxine or T 4 ) and

Symptoms of gastro oesophageal reflux disease, Q. Symptoms of gastro oesoph...

Q. Symptoms of gastro oesophageal reflux disease? Most commonly, people with GERD complain of heartburn, a painful or uncomfortable feeling in the chest, which may radiate to t

What are the substances transferred from the mother to fetus, What are the ...

What are the main substances transferred from the mother to the fetus through the placenta? And from the fetus to the mother? From the mother to the fetus the major transferre

Vitamin, fish liver oil is rich in which vitamin ???

fish liver oil is rich in which vitamin ???

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd