cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Synapse - nervous system, Synapse   Nervous System Structural feature ...

Synapse   Nervous System Structural feature of unique importance in the nervous tissue is the synapse. A synapse is the anatomical site at which axon terminal processes of one

Explain a reason these cells are used instead of outer body, The cells lini...

The cells lining the inside of the cheek are frequently removed for making observations of basic cell structure. The cells are from stratified squamous epithelium. Explain a reason

Marine biology, What are the four characteristics that all chordates have ...

What are the four characteristics that all chordates have at some stage of their lifecycle ?

Jaber, What is genetic drift?

What is genetic drift?

What happens to the movement of molecules at equilibrium, What happens to t...

What happens to the movement of molecules at equilibrium? At equilibrium, the movement of molecules continues, but because there is no concentration gradient, there is no net m

Abscisic acid, Abscisic Acid Abscisic acid (ABA) as a naturally occurr...

Abscisic Acid Abscisic acid (ABA) as a naturally occurring growth inhibitor was discovered through independent investigations of different physiological phenomena in two diffe

Phloem, discrib mechanism of phloem transport

discrib mechanism of phloem transport

Explain the term active transport, Explain the term active transport? ...

Explain the term active transport? Active Transport :  At intervals, protein assemblies involved in selective, or active transport of materials are inserted into the cell mem

Diffusion resemblance with enzymatic chemical reactions, Q. How does facili...

Q. How does facilitated diffusion present resemblance with enzymatic chemical reactions? One of the main examples of facilitated transport is the entry of glucose from the bloo

Piciculture, PROJECTS THAT HAVE TO BE DONE IN SCHOOL ABOT PICICULTURE

PROJECTS THAT HAVE TO BE DONE IN SCHOOL ABOT PICICULTURE

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd