cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

What are the problems regarding terrestrial environment, Q. What are the pr...

Q. What are the problems that vertebrates needed to solve to adapt to the terrestrial environment since they came from the aquatic habitat? How evolution does solve those problems?

Explain lock and key model, Fischer 's template or lock and key model ...

Fischer 's template or lock and key model According to this model, the catalytic site of the enzyme has a proper conformation compatible to a specific substrate even  in  the a

Major organic molecules for living beings, Q. What are the major purposes o...

Q. What are the major purposes of the organic molecules for living beings? Organic molecules, like, lipids, carbohydrates and proteins perform several functions for living orga

Explain about the effect of nutrient and drug interaction, Explain about th...

Explain about the Effect of Nutrient and Drug Interaction? Medicines can treat and cure many health problems. Nevertheless, do you recall your doctor advising or recommending c

Preservation of species diversity, Q. Preservation of species diversity? ...

Q. Preservation of species diversity? Genetic diversity is, thus, important for the preservation of species diversity, and hence biological diversity. A knowledge of the variab

Control of the cell cycle, The cell cycle is controlled by a cyclically ope...

The cell cycle is controlled by a cyclically operating set of reaction sequences that both trigger and coordinate key events in the cell cycle • The cell-cycle control system is

Water movement through the integument, Normal 0 false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

How many possible codons are there, A codon comprise three nucleotides. The...

A codon comprise three nucleotides. There are four possible nucleotides, each of which has an equal probability of being in any one of three spots. How many possible codons exist?

Explain taxonomic hierarchy, Taxonomic Hierarchy The sheer complexity o...

Taxonomic Hierarchy The sheer complexity of organisms seems so bewildering that some people believe, life to be unfathomable by the rational mind. Biologists constantly chip aw

Describe protocol that are used for endotracheal intubation, Describe Proto...

Describe Protocol that are used for Endotracheal Intubation in ICU Intubation is a three person procedure; skilled assistance is mandatory: • "Top end" intubator who coordi

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd