cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Potential and excitation threshold for neurons, Q. How different are the co...

Q. How different are the concepts of action potential, resting potential and excitation threshold concerning neurons? Action potential is the maximum positive voltage level ach

Adolescence, ADOLESCENC E  - It is the period of growth from the onset ...

ADOLESCENC E  - It is the period of growth from the onset of puberty to the completion of growth & maturity. It extends between 12- 20 yrs. It is a period of rapid physical

Define functions of pyridoxine, Define Functions of Pyridoxine? There a...

Define Functions of Pyridoxine? There are three different forms of vitamin B6, namely pyridoxine, pyridoxarnine, and pyridoxal. All three must be phosphorylated and the 5'-phos

Anomalies related to oesophagus and stomach, Anomalies Related to Oesophagu...

Anomalies Related to Oesophagus and Stomach   Let us discuss briefly the anomalies of oesophagus and stomach. These are summarised as  follows: Associated Problems  1)  P

What is the senses explain their vision , What is the Senses explain their ...

What is the Senses explain their Vision ? Vision :  The human eye consists of structures which evolved together to focus light on a sensitive area called the retina. Light f

Explain about periimplant probing depth, Periimplant Probing Depth In c...

Periimplant Probing Depth In contrast to natural teeth, for which the average periodontal probing depth (PD) has been reported, the physiologic depth of the periimplant sulcus

Intro to Genetics and Punnett Squares , "Height" is an example of a gene, b...

"Height" is an example of a gene, but "Short" is an example of...

Define briefly about the pyridoxine vitamin, Define Briefly about the Pyrid...

Define Briefly about the Pyridoxine vitamin B 6 ? Pyridoxine or vitamin B 6 is one of the B complex vitamins which prevents and cures dermatitis in rats fed on vitamin B 6 de

What do you mean by st hystersis, Q. What do you mean by ST Hystersis? ...

Q. What do you mean by ST Hystersis? Ans. The rate of recovery of ST depression has been recognized as a measure of ischaemia severity for many years. When comparing the m

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd