cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Protozoa, what are the disadvantages of protozoa

what are the disadvantages of protozoa

Plant tissues responsible for the supporting of the plant, Which are plant ...

Which are plant tissues responsible for the supporting of the plant? The plant supporting tissues are the sclerenchyma and the collenchyma. The collenchyma is made of elonga

The eye refract the light, Which parts of the eye refract ('bend') the ligh...

Which parts of the eye refract ('bend') the light in such a way as to form an image on the retina?   The curved surface of the cornea, and the aqueous humour enclosed by it,

Morphogenetic movements, Morphogenetic Movements Gastrulation is a dy...

Morphogenetic Movements Gastrulation is a dynamic process including a variety of coordinated movements of cells of dissimilar areas of the blastula. The movements of cells in

Explain phylogenetic or cladistic classification, Phylogenetic or Cladistic...

Phylogenetic or Cladistic Classification Phylogeny plays a great role in classification. It is the  appropriate theoretical background for taxonomy and is quite essential in exp

Describe the regulation of pyruvate dehydrogenase, Describe the regulation ...

Describe the regulation of pyruvate dehydrogenase through covalent modification. PDH exists in 2 forms :  Inactive, phosphorylated and Active, dephosphorylated. The active form

Is protein collagen has a high proportion of glycine, The protein collagen ...

The protein collagen has a high proportion (20% or more) of A. asparagine and glutamine B. proline and hydroxyproline C. glycine D. both A and B E. both A and C F

How to reduce calorie intake from fat, How to reduce calorie intake from fa...

How to reduce calorie intake from fat? Efforts should be made to increase the nutrition knowledge of the general public through mass media. The foods with lower fat co

How is this neural organization important for the diversity, Is the nervous...

Is the nervous system in platyhelminthes more or less sophisticated than in cnidarians? What are the main neural structures found in flatworms? How is this neural organization impo

Pathophysiology of constrictive pericarditis, Q. Pathophysiology of constri...

Q. Pathophysiology of constrictive pericarditis? Ans. The thickened and rigid pericardium causes constriction of the heart and restricts ventricular dialatation and diasto

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd