cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Can you explain secondary metabolites, Q. Can you explain Secondary Metabol...

Q. Can you explain Secondary Metabolites? Secondary metabolites or secondary plant products are those macromolecules that lack nitrogen and arc of restricted occurrence and the

Body fluids of marine teleost, Body Fluids of Marine Teleost The body ...

Body Fluids of Marine Teleost The body fluids of marine teleost are hypotonic to seawater, so these fishes lose water to the environment especially across the gill epithelium.

How can coacervates formed of phospholipids or polypeptides, How can coacer...

How can coacervates be formed of phospholipids or polypeptides? Phospholipids are amphipathic molecules, i.e., they present a polar portion and a nonpolar portion. In contact w

Which compounds results from cyclization of glucose, Which of the following...

Which of the following compounds results from cyclization of glucose? Select one: a. alpha-D-glucopyronose b. beta-D-glucopyronose c. alpha-D-glucofuranose d. beta-D

What do you mean by handrail support, Many patients who are weak, fearful o...

Many patients who are weak, fearful or shot of breath find it essential to hold tightly to the handrail while walking. If accurate aerobic information is to be obtained handrail su

Flatworms, What are the adaptions flatworms neeed in order to live in their...

What are the adaptions flatworms neeed in order to live in their environment?

Change in symptoms of aortic stenosis, Q. Change in symptoms of aortic sten...

Q. Change in symptoms of aortic stenosis? Asymptomatic severe aortic stenosis patient needs to be followed once in a year or even more frequently. Patients with moderate aortic

Use of genital herpes in pregnancy, Use of genital herpes in pregnancy ...

Use of genital herpes in pregnancy Although acyclovir is not approved for treatment of pregnant women, its use during pregnancy has not been associated with an increased risk

Describe the importance of neuropsychological tests, Describe the importanc...

Describe the importance of Neuropsychological tests Neuropsychological tests are generally thought of as assessment instrument for a variety of cognitive, perceptual, and motor

Endosperm of gymnosperms and the endosperm of angiosperms, How dissimilar a...

How dissimilar are the endosperm of gymnosperms and the endosperm of angiosperms? In the gymnosperms the endosperm is haploid (n), it is called as primary endosperm. In the ang

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd