cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

What are zymogens, What are zymogens? Zymogens, or proenzymes, are enzy...

What are zymogens? Zymogens, or proenzymes, are enzymes secreted in inactive form. Under some conditions a zymogen shifts to the active form of the enzyme. Zymogen secretions i

Initial and the final energy levels in catalyzed reactions, Q. Is there a d...

Q. Is there a difference between the initial and the final energy levels in non-catalyzed and catalyzed reactions? The catalysis does not modify the energetic state of reagents

What result from unbridled continued of the population, Relative to carryin...

Relative to carrying capacity, what may result from unbridled continued growth of our population.

Define japanese criteria for functional foods, Define Japanese criteria for...

Define Japanese criteria for functional foods? The Japanese criteria for functional foods include: They are food (not capsules, pills/powder) on the basis of naturally o

Define the nutritional and functional role of nickel, Minerals :-Nickel  ...

Minerals :-Nickel  Food Source      Plant foods  Nutritional Functional role Essential nutrient: Deficiency in humans unknown. Catalyst: hydrogenation in

Hibernation and aestivation, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Explain endonucleases, Explain Endonucleases Endonucleases  are  nucle...

Explain Endonucleases Endonucleases  are  nucleases present  in  the pancreatic juice like RNAase  andDNAase. These hydrolyze internal phosphodiester bonds which are located m

Give choice of catheters for ventriculography, Q. Give Choice of Catheters ...

Q. Give Choice of Catheters for Ventriculography? 1) Pigtail Catheter: This catheter developed by Judkins has end hole and side holes. The end hole permits insertion of the c

Theory of evolution and its rival theories, what is the problem that the th...

what is the problem that the theory of evolution and its rival theories try to solve?

What is a pipette and how is it used, What is a pipette and how is it used?...

What is a pipette and how is it used? There are lots of types of pipettes (or pipette), but most are basically a fancier version of a medicine dropper or eye dropper. They are

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd