cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Guidelines for diabetic patients for healthy coping, Q. Guidelines for Diab...

Q. Guidelines for Diabetic Patients for Healthy Coping? Generally, a diabetic patient adapts to day to day stress effectively, as they accept their disease condition with the s

Clinic blood pressure management, Raised blood pressure is a major risk fac...

Raised blood pressure is a major risk factor for cardiovascular disease. The higher the blood pressure, the higher the risk of stroke, coronary heart disease, kidney disease, heart

Soil pollution, Soil is upper crust of earth which support land plants and ...

Soil is upper crust of earth which support land plants and animals. It is defined as addition of substances to the soil, which adversely affect physical, chemical? And biologica

Categories of secondary inductions, Categories of Secondary Inductions ...

Categories of Secondary Inductions The secondary inductions are categorized into two types. 1) Instructive Interaction : In such type of an interaction the inducing tissue

Response to heavy metal stress, Response to Heavy Metal Stress Severa...

Response to Heavy Metal Stress Several heavy metals emanating from industrial mining and sewage disposal operations contaminate the environment. Cadmium is a common contamina

Define dietary management of sepsis with or without mods, Define Dietary Ma...

Define Dietary Management of Sepsis with or without MODS? Before we begin with the dietary management of patients suffering from sepsis with or without MODS let us read a case

What are the main gas exchange organs of the plants, What are the main gas ...

What are the main gas exchange organs of the plants? How is the process accomplished? In the covering of the leaves and of the primary structure of the stem gas exchange is mad

Ways to adapt to stress, Ways to adapt to stress Now that you know the...

Ways to adapt to stress Now that you know the nature of stress, let us see what type of strategies plants have acquired to adapt to stress. You know that biological functions

Define the role of addition of acetyl groups to histones, Creb binding prot...

Creb binding protein (CBP) is a histone acetyl transferase (HAT) protein. What role does the  addition of acetyl groups to histones play during development? A. The addition of

Importance of the glucose blood level for human health, Q. What is the impo...

Q. What is the importance of the glucose blood level for human health? The glucose blood level (glycemia) must be kept normal if it is abnormally low there is not sufficient gl

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd