cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

What is mutualism, What is mutualism? Mutualism is the ecological inte...

What is mutualism? Mutualism is the ecological interaction in which both participants advantage and that is obligatory for their survival. Mutualism is a harmonious (positive)

Why epiphyseal closure differs between males and females, It is said during...

It is said during puberty rising sex hormones stimulate osteoblasts to produce bone faster than the rate at which epiphyseal cartilage expands. The timing of epiphyseal closure dif

Phases of water, Phases of Water The state of water can be changed from...

Phases of Water The state of water can be changed from gaseous to liquid and liquid to solid and vice versa by the addition or removal of heat energy. To convert one gram of li

Define the nutrient requirements during trauma, Define the Nutrient Require...

Define the Nutrient Requirements during Trauma? Nutritional assessment of the trauma patient is done to determine energy and protein requirements. Basal energy requirements are

Can high blood pressure causes the cardiovascular disease, Q. Can high bloo...

Q. Can high blood pressure causes the cardiovascular disease? Hypertension or high blood pressure: It is also one of the risk factors of cardiovascular disease and is frequentl

Epidermis, EPIDERMIS - Ectodermal in origin. Outer part of skin. Mad...

EPIDERMIS - Ectodermal in origin. Outer part of skin. Made up of stratified epithelium. Branches of sensory nerves present. More branches on lips, tips of fingers. B

Male reproductive disorders due to infections, Male reproductive disorders ...

Male reproductive disorders due to infections Septicemic infections have a greater affinity for vesicular glands than other regions of the reproductive tract viz. prostate, am

Which kind of polarity do water-soluble have, Which kind of polarity do wat...

Which kind of polarity do water-soluble and fat-soluble substances respectively have? Water-soluble substances are polar molecules, i.e., they have electrically charged areas.

What is the function of keratin in the epidermis, What is the function of k...

What is the function of keratin in the epidermis? The epidermis is the outer layer of the skin made of epithelial tissue. In the epidermis there are keratin-secreting cells (ke

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd