cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Reactants of photosynthesis, what are the reactants and the products of pho...

what are the reactants and the products of photosynthesis? under the thylakoid, stroma,and combined

Determine the types of neuropsychology, Determine the types of neuropsychol...

Determine the types of neuropsychology The practise in neuropsychology is roughly divided into two approaches. Some practitioners use standard comprehensive neuropsychological

Determine the implant placement, Implant placement The ideal siting and...

Implant placement The ideal siting and orientation of the implant is dictated by the restorative requirements, but this may have to be modified by the existing ridge morphology

Use pcr to sequence the particular gene, You wish to use PCR to sequence a ...

You wish to use PCR to sequence a particular gene that you have isolated. The sequencing protocol suggests that you use 3.2pmole (pico moles) of your primer in a 2 micro liter volu

Determine the parts consists in our urinary system, Determine the parts con...

Determine the parts consists in Our urinary system Our urinary system consists of following:  Two kidneys  Two ureters  One urinary bladder  One urethra Apart fr

Pathophysiology of endocrine system, What is the relation between posterior...

What is the relation between posterior pituitary gland and diabetes insipidus?

Some common air pollutants: oxides of sulphur, Oxides of sulphur:     ...

Oxides of sulphur:          It includes SO 2 , SO 3 . It is a colorless gas having sharp, pungent and suffocating odour. It is soluble in water and forms H 2 SO 4 . Sourc

List the four phases of meiosis i, List the four phases of meiosis I, and b...

List the four phases of meiosis I, and briefly explain what occurs during each phase Prophase I: DNA coils into chromosomes, the nucleolus and nuclear envelope disappear, the m

Name the hormone that makes the plants more tolerant, In humans, starch dig...

In humans, starch digestion begins in the buccal cavity but stops in the Stomach. Why? Name the two most biodiversity-rich zones of India. Name the hormone that makes the p

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd