cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Describe circulatory system, Circulatory system:  One of the eleven major b...

Circulatory system:  One of the eleven major body organ systems in animals; carbon dioxide, nutrients, transports oxygen, and waste products between the cells and the respiratory s

Accidents in industry workplace, Accidents in Industry Workplace It is...

Accidents in Industry Workplace It is a hard fact to bear in mind that accidents in industry workplace are to be avoided or discarded and efforts in this direction has been in

How to calculate glycemic index, Q. How to calculate glycemic index? If...

Q. How to calculate glycemic index? If you take a certain rood and measure the rise in blood sugar in response to the food consumed in comparison with the response to an equal

Altered glucose metabolism - metabolic response to injury, Explain the Alte...

Explain the Altered Glucose Metabolism - Metabolic Response to Injury? Following injury, hypoglycemia commonly occurs and is related to the severity of the stress. In the ebb p

Phylum , what are diploblastic organisms?

what are diploblastic organisms?

Exon, Exon are those parts of a genomic DNA sequence which will be represe...

Exon are those parts of a genomic DNA sequence which will be represented in final, mature mRNA t A contiguous segment of the genomic DNA which codes for a polypeptide in the gene.

Determinants of vascular tone, The vascular tone increases as the arterial ...

The vascular tone increases as the arterial size decreases. The tone at any time reflects the effects of excitory and inhibitory pathways. Neurotransmitters, physical forces like s

Adolescence, ADOLESCENC E  - It is the period of growth from the onset ...

ADOLESCENC E  - It is the period of growth from the onset of puberty to the completion of growth & maturity. It extends between 12- 20 yrs. It is a period of rapid physical

Injection route - administration of drugs, Injection Route An injecti...

Injection Route An injection is an unpleasant experience for a person at any age and is particularly traumatic to the small child who often lives in fear to the next "need

Class of coclentrata, ????? # 100 ??????????? #Minimum ?????? ?????

????? # 100 ??????????? #Minimum ?????? ?????

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd