cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Integumentary system - nails, NAILS - Cover terminal segment of fing...

NAILS - Cover terminal segment of fingers & toes. Highly vascular dermis under lying the nail is called nail bed . It gives pinkish colour to nail. The small part

Plant breeding, #questitypes of plant breeding on..

#questitypes of plant breeding on..

Hiv, The chances of transmission in female is mor than males

The chances of transmission in female is mor than males

Why the l enantionmer is more dominant, In the Miller-Urey experiment, amin...

In the Miller-Urey experiment, amino acids of bothe the L and D enantionmers. Why is it that the L enantionmer is more dominant in living things?

Briefly explain about food preservation, Briefly explain about Food preserv...

Briefly explain about Food preservation Food preservation,  as you are aware,  is the process in which the perishable food materials are given a suitable physical or chemical t

Define prevention of idd - iodized salt distribution, Define Prevention of ...

Define Prevention of IDD - Iodized salt distribution? Many approaches to reduce iodine deficiency has been formulated. Some of these are reviewed herewith: Iodized salt dist

Detailed explanation of effects of air pollution, On living organism  ...

On living organism           Air pollution causes following effects on living organism: (a)   Effects on humans: 1.      Carbon monoxide reduces the oxygen carrying cap

Explain the carrying capacity in ecology, Explain the carrying capacity in ...

Explain the carrying capacity in ecology? Carrying Capacity :  We have seen that natural populations do not normally achieve the maximum intrinsic rate of natural increase, o

Does ascaris lumbricoides present an intermediate host, Q. Does Ascaris lum...

Q. Does Ascaris lumbricoides present an intermediate host? An Ascaris is a monoxenous parasite, its life cycle is dependent only on one host and so it doesn't have intermediate

Explain chancroid, Chancroid  Chancroid, caused by Haemophilus ducreyi,...

Chancroid  Chancroid, caused by Haemophilus ducreyi,  is rare in the US. A single dose of azithromycin or ceftriaxone is usually effective, but may be less effective in HIV-inf

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd