cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Describe how platelets interact to form a blood clot, Describe briefly how...

Describe briefly how platelets, fibrin and red cells interact to form a blood clot.   Platelets release a substance which, indirectly, causes fibrinogen to be changed to fi

What is cyanosis, What is Cyanosis ? Cyanosis: Cyanosis may be peripher...

What is Cyanosis ? Cyanosis: Cyanosis may be peripheral or central. Peripheral cyanosis almost exclusively involves lips and extremities. Normal neonates may have bluish extrem

Describe about cardiac examination, Describe about cardiac examination? ...

Describe about cardiac examination? Physical examination of the cardiovascular system is mainly discussed under four headings i.e., inspection, palpation, percussion and auscul

What are the major organic molecules for living beings, Q. What are the maj...

Q. What are the major significant organic molecules for living beings? There are many types of organic molecules that are important for the living beings. Particularly importan

Historical review of developmental biology, HISTORICA L REVIEW Anox...

HISTORICA L REVIEW Anoximander (600 B.C.) was the first western philospher who proposed the idea that "living creatures arose from the moist element". Empedocles (500 B.

Muscles, what process provides the energy for muscle contraction?

what process provides the energy for muscle contraction?

Types of advanced plaques, The presence of advanced plaques of types IV and...

The presence of advanced plaques of types IV and Va allows clinical symptoms to develop. Atherosclerosis is a biphasic disease; in the first stage, advanced plaques are generated b

What is the spontaneous generation hypothesis, What is the spontaneous gene...

What is the spontaneous generation hypothesis? The spontaneous generation hypothesys, or abiogenesis, asserts that life on earth has come from nonliving material. For instance,

Pisces, what is the mode of nutrition in pisces

what is the mode of nutrition in pisces

In which field dietetics related, The field of  dietetics can be related to...

The field of  dietetics can be related to: Nutrition care and intervention focused on the individual, and Nutrition care and intervention focused on the group.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd