cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

What would happen to a cell, What would happen to a cell if it was placed i...

What would happen to a cell if it was placed into a hypertonic solution? Into a hypotonic solution? What would happen to a cell if the cell itself was hypertonic to the solution?

Explain the objectives of congenital heart disease, Explain the Objectives ...

Explain the Objectives of Congenital Heart Disease ? After reading this unit, you should be able to: 1 Comprehenced the epidemiologic significance of congenital heart disease (

Hermaphroditism, Hermaphroditism You would have completely appreciated...

Hermaphroditism You would have completely appreciated now that sexual reproduction is definitely the more advanced type of reproduction. It gives for combination of genes from

Define the fehling's soxhlet method (lane-eynon method), Define the Fehling...

Define the Fehling's Soxhlet method (Lane-Eynon method)? This is a titrimetric method that is commonly used in food laboratories to estimate percentage of reducing sugars and t

Who was charles darwin, Who was Charles Darwin? Charles Darwin was an Eng...

Who was Charles Darwin? Charles Darwin was an English naturalist born in 1809 and considered the father of the theory of evolution. At the end of the year 1831, before turning 23

Determine the symptoms of dehydration, Determine the Symptoms of Dehydratio...

Determine the Symptoms of Dehydration? The symptoms of dehydration are: Thirst Loss of appetite Decreased urination Impaired physical performance Nau

Grouping of stem cells-totipotent scs, Grouping of Stem Cells: The rang...

Grouping of Stem Cells: The range of differentiation “Potency” is an important property of Stem cells & serves as the basis for classifying cells as : Totipotent SCs -capa

Explain food applications of gum karaya, Explain Food Applications of gum k...

Explain Food Applications of gum karaya The water absorbing and water-holding capacity of Karaya, together with an excellent acid compatibility made it suitable for its use

Consequences of population growth, T he effects of unlimited population gro...

T he effects of unlimited population growth are as follows: 1.         Increase in unemployment. 2.          Low per capita income. 3.         Low living standard

Mucopolysaccharides, MUCOPO L YSACCHARIDES They are heteropolysacc...

MUCOPO L YSACCHARIDES They are heteropolysaccharides of high molecular weight that are gelatinous is consistency, functioning in lubrication and as sticky substances in or

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd