cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Erythrocytic cycle, Erythrocytic cycle The cycle that takes place i...

Erythrocytic cycle The cycle that takes place in the body of man who is considered as secondary host is called the erythrocytic cycle. The first stage of the cycle begins

Explain the flexibility exercises and its examples, Flexibility Exercises a...

Flexibility Exercises and Examples In  flexibility exercises, movable joints allow one or more of the following types of movements. Flexion - Flexion decreases the joint ang

Name the hormone that makes the plants more tolerant, In humans, starch dig...

In humans, starch digestion begins in the buccal cavity but stops in the Stomach. Why? Name the two most biodiversity-rich zones of India. Name the hormone that makes the p

Precautions should be taken while exercising?, Q. Precautions should be tak...

Q. Precautions should be taken while exercising? Being a diabetic the patient has to take a few precautions. These are: - Initiate the exercise programme gently and then bui

Botany, Suggest a mechanism that would allow a plant to grow better if it h...

Suggest a mechanism that would allow a plant to grow better if it had intermorph neighbors than if it had intramorph neighbors. Think about the degree of genetic similarity between

What is atrial septal defect, What is Atrial Septal Defect ? Fig...

What is Atrial Septal Defect ? Figure: Anatomical location of Am Indication for Surgery :  The presence of an ASD or PAPVC (Partial Anomalous Pulmonary Venous Conn

Explain the relaxing incision - endodontic surgery, Explain the Relaxing In...

Explain the Relaxing Incision - Endodontic Surgery A short vertical incision placed in the marginal and attached gingival, and located at the extent of the Horizontal incision

Proteins Requirement for nutrients, Q. Requirement for nutrients? Ulcer...

Q. Requirement for nutrients? Ulcer is a form of wound which if not healed on time call get perforated and bleed. Adequate protein intake ensures synthesis of new tissues esse

Define molisch's test or alpha naphthol reaction, Define Molisch's Test or ...

Define Molisch's Test or alpha naphthol reaction? The Molisch test is a common test for the existence of carbohydrates. Principle The reaction is due to the formation of

Carbon, I need the parts of the structure carbon. Include elements,monomer...

I need the parts of the structure carbon. Include elements,monomer,polymer, and any sub units

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd