cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

What is a recessive gene, What is a recessive gene? With the birth of a...

What is a recessive gene? With the birth of a child, it gets its genes from both parents. Some genes 'override' other genes. Using hair color as instance: If single parent i

Define the bioavailability of vitamins, Define the Bioavailability of Vitam...

Define the Bioavailability of Vitamins? The term bio availability refers to the overall efficiency of utilization, including physiological and biochemical processes involved in

What is meant by suction force of the plant cell, What is meant by suction ...

What is meant by suction force of the plant cell? Does the suction force facilitate or make difficult the entrance of water into the cell? As the vacuolar solution is hypertoni

Composite endosperm - variants of endosperm, Composite Endosperm - Variants...

Composite Endosperm - Variants of Endosperm In the family Loranthaceae, the development of endosperm is unique. The ovary lacks ovules. The sporogenous tissue located at the b

Explain destruction of large areas of tropical rain forest, How might the d...

How might the destruction of large areas of tropical rain forest have world-wide consequences? As plants consume CO 2 during photosynthesis, extensive deforestation could resu

Human milk composition and infant growth and development, Explain the Human...

Explain the Human Milk Composition and Infant Growth and Development? This sub-section deals with composition of human milk, compares human milk with cow's milk and why human m

Mollusca, 5 classification of mollusco

5 classification of mollusco

Muscular force, 2.?In order to increase muscular force production, muscular...

2.?In order to increase muscular force production, muscular contractions may be modulated in two ways. Explain.

Why do c4 plants have dimorphic chloroplasts, a) Why do C 4 plants have di...

a) Why do C 4 plants have dimorphic chloroplasts? Describe the different steps involved in C 4 photosynthetic carbon cycle in such plants.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd