cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Which type of plant tissue is cork, Which type of plant tissue is cork? ...

Which type of plant tissue is cork? Cork, the material, for example, used to cap wine bottles, is extracted from the suber of a special oak known as cork oak.

Explain prophylaxis of lyme disease, Explain Prophylaxis of lyme disease ...

Explain Prophylaxis of lyme disease No vaccine is currently available to prevent Lyme disease in humans. Avoidance of ticks and use of tick repellents such as DEET or picaridin

What are the functional divisions of the nervous system, What are the funct...

What are the functional divisions of the nervous system? Functionally the nervous system can be separated into the somatic nervous system and visceral nervous system. The so

Reason of population growth, Some of the reason for increase in population ...

Some of the reason for increase in population is: 1.         Decline in death for an increase in life expectancy or longevity. 2.         Better medical facilities

Which are the living beings that carry out photosynthesis, Which are the li...

Which are the living beings that carry out photosynthesis? Which is the cell organelle responsible for the absorption of light for the photosynthesis process in plants and algae?

Explain simple sugar, Simple sugars are monosaccharides consisting of a si...

Simple sugars are monosaccharides consisting of a single polyhydroxy aldehyde or ketone unit. Their general formula is (CH 2 0) n (n=3 to 7).

Prospects of meat products, P r o s p ec ts of meat products The ...

P r o s p ec ts of meat products The demand for convenience meat based fast food is ever increasing due to rapid industrialization and urbanization, higher standards of l

Transcription and processing of prokaryotic rrna, In E.  coli  there  are  ...

In E.  coli  there  are  seven  rRNA  transcription  units  scattered  by  the genome,  each of that contains one copy of every of the 16S, 23S  and 5S rRNA genes and one to four c

Define nutrient requirements and principles of meal planning, Define nutrie...

Define nutrient requirements and principles of meal planning? In this , we started our discussion with the school age and its characteristics. We learnt about the physical grow

Watson - crick model how many polynucleotide chains, Q. As per the Watson -...

Q. As per the Watson - Crick Model how many polynucleotide chains does a DNA molecule have? The DNA molecule is formed by two polynucleotide chains bound in antiparallel mode (

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd