cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Which is the type of nitrogen waste birds produce, Which is the type of nit...

Which is the type of nitrogen waste birds produce? Why does this feature, besides being an adaptation to the terrestrial environment, also mean an adaptation to flight? Birds a

Explain intubation and ventilation, Explain Intubation and Ventilation ? ...

Explain Intubation and Ventilation ? If supplemental oxygen therapy or non-invasive ventilation do not help, the next step would be to intubate and commence mec

Pathogenesis, Even though association between GAS pharyngitis and the ARF i...

Even though association between GAS pharyngitis and the ARF is fairly well established, the exact pathogenic mechanisms are not clearly understood. However, two mechanisms are post

Mr, Define animals

Define animals

Results of pericardial diseases, Results :  Pericardiectomy used to have a...

Results :  Pericardiectomy used to have a mortality of 10-15 per cent in the earlier era. At present it is around 3 to 5 per cent and does not approach 0 per cent even though it

Falling death rate, State the changes in society which could contribute to ...

State the changes in society which could contribute to a falling death rate. The changes in society which could contribute to a falling death rate are:  (i) improvements in

Endocrine system, Endocrine System The nervous system brings about in...

Endocrine System The nervous system brings about integration and co-ordination of several activities of the animal. The afferent stimuli from several sense organs are brought

Defence mechanisms of the host, Defence mechanisms of the host The hea...

Defence mechanisms of the host The healthy animal is protected from pathogenic organisms present in the environment by virtue of the effective immune mechanism present from bi

How are secondary cell walls different from primary cell, How are secondary...

How are secondary cell walls different from primary cell walls? Primary cell walls are assembled on the surface of the plasma membrane whereas the cell is growing. They can gro

Which cell organelles are well-developed in secretory cell, Q. Which cell o...

Q. Which cell organelles are well-developed in secretory cells? In secretory cells, like the secretory cells of endocrine glands, organelles related to production, "exportation

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd