cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Do membranes form only the outer wrapping of cells, Normal 0 fa...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Why do cells of the nephron tubules, Q. Why do cells of the nephron tubules...

Q. Why do cells of the nephron tubules present a great amount of mitochondria? The cells of the tubule wall have high number of mitochondria because many substances are secrete

Explain the cell cycle in details, Explain the cell cycle in details? M...

Explain the cell cycle in details? Most cells of higher organisms follow a cyclic pattern of division. The bulk of the cycle consists of the stage known as interphase, the peri

Common disease of pig, COMMON DISEASE OF PIG  - 1 .       FOOT AND MO...

COMMON DISEASE OF PIG  - 1 .       FOOT AND MOUTH DISEASES 2 .       ANTHRAX 3 .       TUBERCLOSIS 4 .       SWINE FEVER - Charactrized by high fever, d

Dideoxy sequencing, Dideoxy Sequencing is the enzymatic determination and ...

Dideoxy Sequencing is the enzymatic determination and consideration of DNA or RNA sequence by the rechnique of Sanger and colleagues, based on incorporation of the chain terminati

Zoonotic diseases and trade, Zoonotic diseases and trade Disease and t...

Zoonotic diseases and trade Disease and trade have a long-standing, interwoven history. During the 14th century, Europeans realized the value of exotic nature of goods and mat

What are the bone grafting issues, Bone grafting issues Planning of the...

Bone grafting issues Planning of the case is very critical. Any bone defect in the aesthetic zone should be evaluated prior to implant placement in order to obtain a prosthetic

Define methods for determining ph - nutritional biochemistry, Define Method...

Define Methods for determining pH? There are two general methods for determining pH of a solution. One is the colorimetric method and the other most common method is the use of

Mosaic and regulative eggs, Mosaic and Regulative Eggs Eggs as well a...

Mosaic and Regulative Eggs Eggs as well as early cleavage embryos are occasionally divided into two categories: i) Mosaic or determinate embryos and ii) Regulative or i

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd