cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

How to measure blood pressure, Q. How to measure Blood Pressure? With t...

Q. How to measure Blood Pressure? With the onset of exercise, the resistance to blood flow through contracting muscles decreases significantly and results in a fall in the peri

Define subphylum chelicerata, Define Subphylum Chelicerata - classes Arachi...

Define Subphylum Chelicerata - classes Arachinda AM merostomata? The subphylum Chelicerata includes familiar horse shoe crab, spiders, scorpions, ticks and mites. 1. Body consis

Nutrition, You will be learning about vitamins and minerals (micronutrients...

You will be learning about vitamins and minerals (micronutrients) over a 2 week span. Remember phytochemicals from the Controversy section of chapter 2? They are not vitamins, b

What is the goal of biological classification, The goals of biological clas...

The goals of biological classification The world of animal diversity is quite complex and it requires an ability to recognise similarities and differences among organisms. Class

# FUNGAL NUTRITION, Write on spoilage of food products by fungi,nutrition,c...

Write on spoilage of food products by fungi,nutrition,condition for growth,consequencies and prevention.

What is triacylglycerols, A triglyceride TG, TAG, triacylglycerol or triacy...

A triglyceride TG, TAG, triacylglycerol or triacylglyceride is an ester derivative from glycerol and three fatty acids. There are various triglycerides: depending on the oil source

Egg type, why their is different amount of yolk is present in eggs.

why their is different amount of yolk is present in eggs.

Eretmochelys imbricata, Ivory was much in demand for production of a variet...

Ivory was much in demand for production of a variety of jewellery and artifacts in the past. Reptile leather from crocodiles, lizards and snakes has quite a high demand for manufac

What type of feedback loop is, The client was admitted to the hospital with...

The client was admitted to the hospital with hypertension (high blood pressure). The development of arteriosclerosis (plaque buildup in the arteries) has increased peripheral resis

Explain procedure for preparation of general purpose media, Explain the Pro...

Explain the Procedure for Preparation of General Purpose Media Procedure: Now prepare the media following the steps enumerated herewith: 1. Weigh and dissolve each ingredien

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd