cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

What is the difference between taeniasis and cysticercosis, What is the dif...

What is the difference between taeniasis and cysticercosis? Taeniasis is the parasitic disease caused by the adult tapeworm installed within the human intestine. Cysticercos

Modern theory in biology, Modern Theory Naturalistic theory: Accordin...

Modern Theory Naturalistic theory: According to this theory Life originated upon our earth spontaneously from nonliving matter. But there are two significant points in this r

Explaintransketolase, ExplainTransketolase Transketolase with the help...

ExplainTransketolase Transketolase with the help of TPP  and Mg ++   is  required again. This  time  it  transfers carbon 1+2 from xylulose-5-phosphate  to erythrose-4-phospha

Zoology, adaptation and distribution of synaptura (flat fish)

adaptation and distribution of synaptura (flat fish)

What is the genetic family tree, What is the genetic family tree? The G...

What is the genetic family tree? The Genetic family tree is a schematic family tree that shows the biological inheritance of some trait through successive generations. The G

What is symbiosis, Question Write a short note on the following: 1...

Question Write a short note on the following: 1 Gram staining 2 Embden-Meyerhoff-Parnas pathway 3 Interferons 4 Probiotics 5 Pasteurization 6 Endospores

Explain about the saponins, Explain about the Saponins? These are a div...

Explain about the Saponins? These are a diverse group of compounds commonly found in legumes like soybean, lentils, chickpeas, peanuts and alfalfa sprouts. They are also presen

Ascitic fluid collection - specimen collection, Pleural, Pericardial, and A...

Pleural, Pericardial, and Ascitic fluid Collection:      The procedure is called paracentesis . If for pleural cavity is called thoracocentesis , and for pericardial cavity c

Which is the vertebrate class, Q. Which is the vertebrate class that is con...

Q. Which is the vertebrate class that is considered the first entirely terrestrial? Totally independent from the aquatic habitat, The first entirely terrrestrial vertebrate cla

The trp operon, The trp (tryptophan) operon which is shown in the figure wi...

The trp (tryptophan) operon which is shown in the figure will have  five  structural  genes  encoding enzymes  for tryptophan  biosynthesis  with an upstream  Ptrp  (trp promoter)

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd