cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

genes occur on chromosome , In Drosophila, these genes occur on chromosome...

In Drosophila, these genes occur on chromosome 4. H - wild type (normal)     h - hairy F - wild type                   f - frizzled E - wild type                  e - vest

Describe the developmental periods of coronary artery diseas, Describe the ...

Describe the Developmental Periods of coronary artery diseases? The development of coronary artery disease like many other diseases can be divided into the following periods:

What chromosome condition is created, Following fertilization by a normal s...

Following fertilization by a normal sperm, what chromosome condition is created?

1, characters of phylum protozoa

characters of phylum protozoa

Explain the use of chlamydia in pregnancy, Use of Chlamydia in Pregnancy  ...

Use of Chlamydia in Pregnancy  Doxycycline, other tetracyclines and the flour quinolones generally should not be used during pregnancy. Azithromycin appears to be safe in pregn

Evolution of culture, A general agreerncnt anlong nll the human evolutionis...

A general agreerncnt anlong nll the human evolutionists who may have a very radical philosophical hackground and convictions is that man is the result of an extraordinary evolution

Explain directive and non-directive counseling, Directive and Non-directive...

Directive and Non-directive Counseling Directive Counseling  tends  to  be appropriate when  the  counselor  is  aware  of  the problem  and/or  is concerned about  the behavio

Blood supply of the heart, The heart is supplied exclusively by the right a...

The heart is supplied exclusively by the right and left coronary arteries. The coronary arteries are functional end arteries. There is no effective anastamosis between right and le

Scheme of classification of phylum hemichordate, Q. Scheme of Classificatio...

Q. Scheme of Classification of phylum hemichordate? These are about 90 species of hemichordates. All are marine and live in the open sea or in muddy sediments. Hemichordata wer

How dissimilar are cnidarians from poriferans, Q. Concerning tissue complex...

Q. Concerning tissue complexity how dissimilar are cnidarians from poriferans? Poriferans present only some dispersed specialized cells with no tissue differentiation, Cnidaria

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd