cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Explain false positive tests, Q. Explain False Positive Tests? As ST de...

Q. Explain False Positive Tests? As ST depression has been equated with CAD, patients with this finding who have less than a critical coronary narrowing has been called false p

Explain the interaction of vitamin a with iron, Explain the interaction of ...

Explain the interaction of vitamin A with Iron? Iron: Iron status of an individual correlates with vitamin A. The deficiency of  vitamin A has been found to be associated with

What similarities do birds and reptiles share, What similarities do birds a...

What similarities do birds and reptiles share regarding external coverage, reproduction and excretion? Regarding external coverage, birds are same to reptiles as they present

What is ammensalim, Q. What is ammensalim? The Ammensalism is the ecolo...

Q. What is ammensalim? The Ammensalism is the ecological interaction in which an individual harms another without obtaining benefit. The Ammensalism is an inharmonious (negativ

Define excretion of toxin and act as precursor- carbohydrate, Define Excret...

Define Excretion of toxins and Act as precursors? Excretion of toxins: Glucuronic acid, a metabolite of glucose, combines with chemical and bacterial toxins and some normal met

Factors controlling stomatal aperture, Factors Controlling Stomatal Apertur...

Factors Controlling Stomatal Aperture 'Khul Ja Sim Sim' the two rocks slide and the gates to a great treasure open. With these magic words "Ali Baba and Chalis Chor" could ope

Illustrate numerical taxonomy, Q. Illustrate Numerical Taxonomy? Taxono...

Q. Illustrate Numerical Taxonomy? Taxonomy today is, in many dctails, different from what it was a generation ago. The use of computers by taxonomists has established an intere

Show upsloping st-segment, Q. Show Upsloping ST-Segment? There are few ...

Q. Show Upsloping ST-Segment? There are few subjects in exercise literature that has generated more controversy than the significance of up sloping ST-segments. The up sloping

Pathogenesis, Even though association between GAS pharyngitis and the ARF i...

Even though association between GAS pharyngitis and the ARF is fairly well established, the exact pathogenic mechanisms are not clearly understood. However, two mechanisms are post

Minerals and trace elements, Minerals and Trace Elements Oxygen, carbo...

Minerals and Trace Elements Oxygen, carbon, hydrogen and nitrogen are the most common elements that make up 96% of the total weight of a mammal. The next most abundant element

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd