cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Why is pattern baldness more common in men than in women, Why is pattern ba...

Why is pattern baldness more common in men than in women? Pattern baldness is handled by the allele B. Testosterone interacts with the heterozygous genotype (BB′) to make baldn

What are the human tissues affected by malaria, Q. What are the human tissu...

Q. What are the human tissues affected by malaria? What are the main clinical manifestations of the disease? A plasmodium contaminates the human blood causing destruction of RB

Define vacuum production and thermal processing - canning, Define Vacuum pr...

Define Vacuum production and Thermal processing - Canning? Vacuum production - This can be obtained by filling the heated product into the can, by heating the can and content

Myocardial contractility, Inspiration decreases the pressure in the central...

Inspiration decreases the pressure in the central veins and thus increases the pressure gradient between the peripheral and central veins thus augmenting venous return. Skeletal mu

Guidelines for the family members for healthy coping, Q. Guidelines for the...

Q. Guidelines for the Family Members for Healthy Coping? - Patient and the family members should discuss the situation, change in role,time distribution, share of money and oth

State the exocytosis in a skeletal muscle, Exocytosis in a skeletal muscle?...

Exocytosis in a skeletal muscle?   A.  During exocytosis in a skeletal muscle, there will be release of calcium ions from intracellular vesicles in the sarcoplasmic reticulum i

Blunt or non-penetrating injury, Blunt or Non-penetrating Injury When ...

Blunt or Non-penetrating Injury When body is struck by a blunt object such as steering wheel or blows to chest with blunt object. External injury may appear minor but the

Define pacinian corpuscules, Q. Define Pacinian corpuscules? Pacinian ...

Q. Define Pacinian corpuscules? Pacinian corpuscules are an example of sensory receptors scattered deep in the subcutaneous tissue underlying skin or in viscera.  These mech

Cerebellum, why cerebellum more developed in mammals that jump or fly?

why cerebellum more developed in mammals that jump or fly?

How do the cdnas vary from the original genes, How do the cDNAs vary from t...

How do the cDNAs vary from the original genes? A. The inserted cDNA copies lack enhancers, promoters and introns. B. The genomic structures of the original gene and the ins

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd