cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Porifera, Diagrams of organisms in phylum porifera

Diagrams of organisms in phylum porifera

Reproduction, Normal 0 false false false EN-IN X-NONE...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Agro industrial-female reproductive disorders, Heifers As efficient reprod...

Heifers As efficient reproductive performance is essential for economic livestock production, the female calves must grow rapidly to attain sexual maturity, ovulate and be mated b

Fatty acid metabolism, Fatty Acid Metabolism The body has a limited sup...

Fatty Acid Metabolism The body has a limited supply of glucose associative to the energy stored as fat. There are 3 sources of fatty acids for energy metabolism within animals;

How body fat can be measured, How body fat can be measured? The convent...

How body fat can be measured? The conventional golden method of measuring BF% is by underwater weighing. Difference of weight in air and in water gives density, from which the

Show the physical criteria of quality for honey, Q. Show the Physical crite...

Q. Show the Physical criteria of quality for Honey? Colour, crystallization, pH, acidity, water content are some of the criteria used for analysis of honey. These criteria are

Oxidative phosphorylation occurs in the inner membrane, In  eukaryotes,  th...

In  eukaryotes,  the electron  transport  and oxidative phosphorylation occurs in the inner membrane of mitochondria.  These processes re-oxidize the FADH 2 and NADH   which comes

What are the apical meristems, What are the apical meristems? Which kind of...

What are the apical meristems? Which kind of plant growth does this meristem promote? The Apical meristems are those primary meristems found in the apex of the stem as well as

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd