cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

What is av block, Q. What is AV Block ? First Degree AV Block At ...

Q. What is AV Block ? First Degree AV Block At rest commonly disappears with exercise owing to the vagal withdrawal. The development of a prolonged PQ segment after exerc

Integral membrane protein movement and distribution, Various proteins are f...

Various proteins are free to take a move laterally in the plane of the bilayer. One experiment used to represent this included fusing cultured mouse cells with human cells under ap

Explain capillaries of the vascular system, Q. What are the capillaries of ...

Q. What are the capillaries of the vascular system? Capillaries are small blood vessels that perform exchange of substances between the tissues and blood the body Capillaries a

Theory of germplasm, THEOR Y OF GERMPLASM - August Weismann (1834-1...

THEOR Y OF GERMPLASM - August Weismann (1834-1914) criticized the inheritance of acquired characters by putting forward the theory of continuity of germplasm. According

Fermentation, outline the process of yeast fermentation stating all enzymes...

outline the process of yeast fermentation stating all enzymes involved

Which are the beings that form the kingdom animalia, Which are the beings t...

Which are the beings that form the kingdom Animalia? What are the two big groups into which this kingdom is divided? The kingdom Animalia is the animal kingdom. Commonly the ki

Name stems of plants which are leaf like, Which of the below are stems of p...

Which of the below are stems of plants that are leaf like in form, are green and perform functions of leaves. Additionally, they may bear fruit, flowers and temporary leaves?

Show the artificial keys, Q. Show the Artificial Keys? These are based ...

Q. Show the Artificial Keys? These are based on the resemblances and differences of some prominent characters in the taxa. These are based on the phylogenetic relationship b

Membrane that selects based on pore size, A transport process in which phys...

A transport process in which physical (hydrostatic) pressure drives part of a fluid through a membrane that selects based on pore size

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd