cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Meiosis, Why is it necessary to have the second division in meiosis even th...

Why is it necessary to have the second division in meiosis even though cells have already been reduced to haploid number after the first division?

Define skirt fold thickness (spt) method, Define Skirt Fold Thickness (S...

Define Skirt Fold Thickness (SPT) Method? Skin fold measurement is the most widely used field method of body composition assessment. The skin fold (SKF) is an indirect measu

Extra-embryonic membranes and placenta, Extra-Embryonic Membranes and Place...

Extra-Embryonic Membranes and Placenta The extra embryonic membranes like you already know provide nourishment and protection. These membranes are fundamentally similar to th

Explain the birth in human biology, Explain the Birth in human biology? ...

Explain the Birth in human biology? In humans, birth of the infant occurs about 270 days after conception. The period during which the uterus contracts to expel the newborn

Define mechanism for provide protection against colon cancer, Define Mechan...

Define Mechanisms for Provide protection against colon cancer? Several plausable mechanisms have been formulated by which fibre may provide protection against colon cancer. The

Conventional cabg on cardiopulmonary bypass, Conventional CABG on Cardiopul...

Conventional CABG on Cardiopulmonary Bypass   Chest is opened by midline incision and median sternotomy. Simultaneously saphenous vein or radial artery is harvested. 'T

How much dna is in each stage, In meiosis - starting from stage G1 through ...

In meiosis - starting from stage G1 through the completion of meiosis, how much DNA is in each stage/phase when referring to the nucleus of spermatagonia?

Explain the protection of erythrocytes and cell membrane, Explain the Prote...

Explain the Protection of erythrocytes and cell membrane? Protection of erythrocytes: Vitamin E protects erythrocytes from haemolysis by the production of oxidizing agents

What are the main bryophyte groups, Q. What are the main bryophyte groups? ...

Q. What are the main bryophyte groups? The major bryophyte groups are the mosses, the hornworts and the liverworts.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd