cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

Define the symptoms of yersinia enterolytica infection, Normal 0 ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4 Define the Symptoms

Cardiomyocytes and motor neurons, Describe the mechanisms and genetic regul...

Describe the mechanisms and genetic regulations that underpin how two cells such as cardiomyocyte and a motor neuron have identical gnomes, yet are functionally and structurally di

#nervous system, 6. What is the function of the myelin sheath? Do all axons...

6. What is the function of the myelin sheath? Do all axons present a myelin sheath?

Explain in detail about gene therapy, Explain in detail about Gene therapy ...

Explain in detail about Gene therapy Gene therapy technology as a whole has yet to be proven and it's meeting a number of stumbling blocks, so may have little impact (low succe

Five kingdom classifications, Five Kingdom Classifications Biology, as...

Five Kingdom Classifications Biology, as you know, is the study of life, living things, and their relationship to one another and to their environment. This branch of Science

Define buffers and buffer solutions, Define Buffers and Buffer Solutions? ...

Define Buffers and Buffer Solutions? Solutions containing both weak acid and their salts or solutions containing weak hydroxides and their salts are referred to as buffer solut

Define effect of dietary fibre expect physiological effects, Define effect ...

Define effect of dietary fibre expect physiological effects? In addition to all the physiological effects mentioned above, dietary fibre may exert other effects. Acetate and pr

Cartilaginous and the osseous tissue, Q. Which kind of tissue are the carti...

Q. Which kind of tissue are the cartilaginous and the osseous tissue? The osseous and the cartilaginous tissues are considered connective tissues since they are tissues in whic

Carbohydrates, Carbohydrates These form about 1%part of protoplasm, the...

Carbohydrates These form about 1%part of protoplasm, they are comparatively simpler compounds of carbon, hydrogen and oxygen in the ratio 1:2:1 The ratio hydrogen to oxygen is

Define secondary and tertiary structure of a protein, Is it expected that a...

Is it expected that a change in the primary, in the secondary or in the tertiary structure of a protein will produce more functional consequences? Any change of the protein str

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd