cloning, Biology

Assignment Help:
there are a restriction map of a plasmid and a human gene of known sequence. Your task is to clone the human liver cDNA for this gene so that the start codon is closer to the EcoRI site than the PstI site of the polylinker/MCS, but you also need to get rid of the promoter+RBS+Hin insert already in the plasmid. Explain how you would accomplish the cloning using a series of numbered steps. Note that in the plasmid, the (1) refers to the frequency of these sites in the plasmid plus existing insert. In the gene’s restriction map, all the relevant restriction sites are indicated if they are present

Related Discussions:- cloning

What is the energy efficiency of photosynthesis, What is the energy efficie...

What is the energy efficiency of photosynthesis? a) What area is occupied by each molecule? b) Calculate the molecular absorption cross section in units of A 2   A cr

Does the environment exert an influence on the phenotype, Does the environm...

Does the environment exert an influence on the phenotype? A phenotype may be changed (compared to the original situation conditioned by its genotype) by nongenetic means. Examp

Reed-swamp stage - hydrarch, Reed-Swamp Stage - Hydrarch This stage is...

Reed-Swamp Stage - Hydrarch This stage is also known as amphibious stage, as the plants of the community are rooted but most parts of their shoots remain exposed to air. Speci

Synthesis of starch, Thus animals store surplus carbohydrate as glycogen; p...

Thus animals store surplus carbohydrate as glycogen; plants do so in form of starch. Starch is producing in the stroma of chloroplasts and stored there as starch grains. The Starch

Botany, cellular endosperm diagrammatic view

cellular endosperm diagrammatic view

BNA and RNA, Compared to the original sequence what type of mutation occurr...

Compared to the original sequence what type of mutation occurred on the following DNA sequence? Original: TACGAATTCGATAC Mutant: TACASSTTCCATAC

Show defense mechanism when inflammation fails, Q. What is the defense mech...

Q. What is the defense mechanism that begins to work when inflammation fails to stop an infection? If the inflammatory attack is not sufficient to halt the infectious process t

Biosynthesis of hemes and chlorophylls, In animals, some of bacteria and fu...

In animals, some of bacteria and fungi, and the first step in tetrapyrrole synthesis is the condensation of the amino acid glycine with succinyl  CoA (an intermediate  of the citri

Briefly explain about the chromium, Briefly Explain about the Chromium? ...

Briefly Explain about the Chromium? As you will go through this section, you will realize that compared to other minerals, the essentiality of chromium was recognized very late

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd