Clone (verb), Biology

Assignment Help:

Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.

One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.


Related Discussions:- Clone (verb)

Measures to be taken to avoid fire and in event of a fire, (a)    Bedroom ...

(a)    Bedroom :                    ·           Do not place room heaters near any inflammable materials.                  ·           Do not use heaters

Assignment of botany, habitat, habit, cell wall chemistry, reason why cell ...

habitat, habit, cell wall chemistry, reason why cell wall like this of Archaebacteria , eubacteria,fungi,algae,bryophytes ,pteridophyes, gymnosperms , angiosperms..

Diarrhoea, Diarrhoea   Diarrhoea refers to frequent passage of loose wa...

Diarrhoea   Diarrhoea refers to frequent passage of loose watery stools. Acute diarrhoea often called as acute gastroenteritis  is particularly a leading cause of mortality in

Acute aortic regurgitation-types of regurgitation, Acute Aortic Regurgitati...

Acute Aortic Regurgitation :  The most dramatic presentation occurs in dissection of the aortic root. Endocarditis of the native or prosthetic aortic valve may also lead to a

What are the problems that vertebrates required, What are the problems that...

What are the problems that vertebrates required to solve to adapt to the terrestrial environment as they came from the aquatic habitat? How evolution does solve those problems?

Genetics, Explain the difference between meiosos and mitosis

Explain the difference between meiosos and mitosis

Chara, Assighments of chara

Assighments of chara

Sea Lampreys, #questiHow do the non parasitic lampreys interact with other ...

#questiHow do the non parasitic lampreys interact with other members of their ecosystem in comparison to their parasitic cousinson..

What is suppuration, Suppuration High number of PMN cells have been det...

Suppuration High number of PMN cells have been detected around implants that are associated with severe signs of mucosal inflammation. Several studies have shown the presence o

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd