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Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.
One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.
Intracellular sodium concentration of the motor neuron A complete motor neuron is removed from a frog and placed in normal physiological saline at 1 AM. The neuron is healthy.
Ecology is divided into two fields: (a)Autecology (b) Synecology (a) Autecology: it deals with life history, population dynamics, behavior etc of a single
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TYPES OF PLASTIDS (1 ) LEUCOPLASTS These are present in ground parts of plants, internal parts of herbaceous stems and deep tissues of plants where sun light is not avail
What is Phylum Nematoda - Roundworms? Nematodes are commonly known as the roundworms. They have a pseudocoelom type of body cavity, and they have a digestive tract that opens t
In the human body, the potassium ion can pass easily through cell membranes, yet the potassium ion concentration is higher inside many cells than it is outside these cells. Could t
State the term - Halsted Reitan varies with the particular test Scoring for the Halsted Reitan varies with the particular test, such that individual scores may be expressed in
Packs, pouches, and cassettes are sometimes wet after completing the sterilization process. This is a problem usually associated with the use of paper or paper/ plastic wraps in t
Phylum Apicomplexa - Protozoan Characteristic set of organelles (apical complex) asssociated with the anterior end present in some developmental stages. Cilia and flagella abs
In biology, Homeostasis is the ability of an open program to manage its internal environment to sustain constant circumstances through several energetic stability improvements mana
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