Clone (verb), Biology

Assignment Help:

Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.

One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.


Related Discussions:- Clone (verb)

Which of the groups is not ionizable, Which of the following groups is NOT ...

Which of the following groups is NOT ionizable? Select one: a. Guanidinium b. Imidazole c. Phosphoryl d. Amine e. Aldehyde

Stable atoms have no vacancies in their outer shell, Select all that are tr...

Select all that are true/correct: Stable atoms have no vacancies in their outer shell. Hydrogen is more stable than carbon Helium is more stable than carbon Helium is more stable t

Define nutritional availability - microbial survival, Define Nutritional Av...

Define Nutritional Availability - Microbial Survival and Growth? Food borne microbes are chemotrophs. Generally the types of microorganisms present on a particular food are the

Acute bronchitis, Acute Bronchitis: Acute bronchitis is inflammation  ...

Acute Bronchitis: Acute bronchitis is inflammation  of one or more bronchi  and occurs specifically  in children younger than  four year?.  It is usually associated with previ

How can total count can be obtained?, How Can Total Count Can Be Obtained? ...

How Can Total Count Can Be Obtained? Total count can be obtained microscopically by using fluorescent dyes such as acridine orange or DNA stain DAPI. The counts obtained micros

Should human gene therapy be continued, Debate over ethics of using gene th...

Debate over ethics of using gene therapy for treating human beings started since the discovery of recombinant DNA technology. The mere fact that it involves the alteration of DNA w

Explain about the prebiotics, Explain about the Prebiotics? Ingredients...

Explain about the Prebiotics? Ingredients/compounds that have a beneficial effect on microflora in the large intestine of the host e.g. fibre, fructo oligosaccharides, lactulos

How can the immune memory lead to the efficacy of vaccines, Q. How can the ...

Q. How can the immune memory lead to the efficacy of vaccines and also produce allergies? Vaccines are controlled inoculations of fragments of inactive infectious agents or of

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd