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Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.
One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.
Define the Nutrient Requirements during Trauma? Nutritional assessment of the trauma patient is done to determine energy and protein requirements. Basal energy requirements are
why left brain controls right side of the body and right brain controls left side of the body....?????
The evolutionary species concept which identifies a species as a "lineage (i.e. ancestor descendant sequence of population) evolving separately from others with its own unitary ro
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WHICH SOIL WILL BE FRUIFUL FOR GROWING LENTILS AND OTHER PULSES
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