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Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.
One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.
EXCRETION IN PRAWN- A pair of antennary gland or green gland and a median renal sac present. Green glands lie at the base of antennae & open out by renal aperture.
Enumerate about the Reitan Aphasia Screening Test This test serves two purposes in that it contains both copying and language-related tasks. As an Aphasia screening procedure,
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Roles of Abscisic Acid Abscisic acid (ABA) is a particularly interesting hormone with regard to the regulation of its own levels. Its levels rise and fall dramatically in seve
Convergent evolution is the development of the similar type of structures in distantly related organisms as a result of adapting to similar environments or strategies of life. For
a) What are the two types of chemical compound which combine to form a lipid? b) What elements are present in a lipid? a) Lipids are formed from the combination of
Gametophytic Incompatibility In GSI systems callose deposition is not evident on the stigma but is very conspicuous in the pollen tube. Sometimes the callose deposition occurs
A solution was prepared by dissolving 25g NaOH powder in 100 ml water. Using not more than 15 ml water, devise a series of dilutions that will give a concentration of 5mg/ml NaOH.
Neurosecretory Systems Almost all groups of multicellular animals have been investigated to find out if neurosecretory cells are present in them. Such investigations have sho
Q. What is the excitation threshold of a neuron? How does this threshold relate to the "all-or-nothing" rule of the neural transmission? The excitation threshold of a neuron is
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