Clone (verb), Biology

Assignment Help:

Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.

One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.


Related Discussions:- Clone (verb)

Define nutritional management of severe anorexia nervosa, Define nutritiona...

Define nutritional management of severe anorexia nervosa? The nutritional management of severe anorexia nervosa is therefore, considered in terms of three consecutive phases:

Neurotransmitters to neuromuscular junctions, Blood functions to maintain h...

Blood functions to maintain homeostasis in the human body through all but which of the following: Answer moving carbon dioxide away from cells following completion of aerobic metab

Determine the viscosity of solutions, Viscosity Viscosity, as you may ...

Viscosity Viscosity, as you may already know, is associated with fluid flow.  It is the internal friction which tends to bring to rest portions of the fluids moving relative to

Stereoisomers , Glyceraldehyde has a one asymmetric carbon atom the central...

Glyceraldehyde has a one asymmetric carbon atom the central one and so two stereoisomers  which is also known as optical isomers are possible, which is two forms of glyceraldehyde,

Blank titration for determination of iodine number of lipids, Define Blank ...

Define Blank titration for Determination of the Iodine Number of Lipids? Using an autopipette, measure 25 ml of Hanus iodine reagent accurately and transfer into a 250 ml iodin

Define cause of vitamin a deficiency - infections, Define cause of vitamin ...

Define cause of vitamin a deficiency - Infections? During acute infections, vitamin A intake in preschool children is reduced due to impaired appetite and impaired vitamin A ab

Symptoms of enteropathogenic escherichia coli, Symptoms: The E. coli gastro...

Symptoms: The E. coli gastroenteritis syndrome is caused by the ingestion of 10 6 -10 10 viable cells/g that must colonize the small intestine and produce  enterotoxin. The syndr

Describe primitive anaerobic eukaryotic cells, Q. How can the hypothesis th...

Q. How can the hypothesis that asserts that chloroplasts as well as mitochondria were primitive prokaryotes that related in mutualism with primitive anaerobic eukaryotic cells be c

The digestive system, fat digestion requires two steps, what are the steps ...

fat digestion requires two steps, what are the steps and what enzymes are use to accomplish

Feed adulterants, Feed adulterants Adulteration of raw material is cre...

Feed adulterants Adulteration of raw material is creating a serious problem for manufacturing the good quality feed as the trading of raw ingredients is totally in unorganized

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd