Clone (verb), Biology

Assignment Help:

Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.

One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.


Related Discussions:- Clone (verb)

Which kind of plant tissue is cork, Which kind of plant tissue is cork? ...

Which kind of plant tissue is cork? The Cork is the material for instance, used to cap wine bottles, and is extracted from the suber of a special oak called cork.

Synthesis and storage of hormone, Synthesis and Storage of Hormone The...

Synthesis and Storage of Hormone The synthesis and storage of hormone depends on the nature of the hormone. Steroid hormones are secreted in diffuse molecular form and usually

Polynephritis in cattle, Polynephritis in cattle Contagious bovine pol...

Polynephritis in cattle Contagious bovine polynephritis is a specific infection of urinary tract of cattle. The causative agent is Corynebacterium renale, a rod shaped gram-po

Describe pressure controlled ventilation, Describe Pressure Controlled Vent...

Describe Pressure Controlled Ventilation (PCV) This is a pressure cycled mode (machine delivers a set pressure - the tidal volume generated depends on how compliant the lungs

Permanent partial disability - injury from an accident, Permanent Partial D...

Permanent Partial Disability - Injury from an Accident Permanent partial disability restrains the worker by doing the job he has been doing. He recovers from the injury in th

Radiographic evaluation - criteria for endosteal implants, Radiographic Eva...

Radiographic Evaluation - criteria for endosteal implants Surgical and interventional imaging involves imaging the patient  during and immediately after surgery and during the

Describe the number of implants, Number of Implants The use of more imp...

Number of Implants The use of more implants decreases the number of pontics and the associated mechanics and strains on the prosthesis, and dissipates stresses more effectively

Trinomial system, who proposed trinomial system of classification? in ALLEN...

who proposed trinomial system of classification? in ALLEN modules ans is Huxley & Stricklandt but in AAKASH its given Lamarck so what is correct?

Dietary management of diverticular disease, Q. Dietary Management of divert...

Q. Dietary Management of diverticular disease? Most of the diseases which we have discussed so far do not require any major changes in the nutrient intake. The patients general

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd