Clone (verb), Biology

Assignment Help:

Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.

One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.


Related Discussions:- Clone (verb)

What are the main constituents of the earth atmosphere, What are the main c...

What are the main constituents of the earth's atmosphere in our time? The present atmosphere of the earth is constituted mostly of molecular nitrogen (N2) and molecular oxygen

What is initiation delay, Which of the following is the best definition of ...

Which of the following is the best definition of the initiation delay that is seen in all organisms? A. Initiation delay is explained as the time it takes for the pre-initiatio

Define work capacity that is relevant to sports nutrition, Define Work Capa...

Define Work Capacity that is relevant to sports nutrition? There are generally two areas of work capacity that is relevant to sports nutrition one is aerobic capacity and the o

What is oxidation and reduction, What is Oxidation and Reduction? Oxid...

What is Oxidation and Reduction? Oxidation and Reduction :   Most people are familiar with oxidation as it pertains to the rusting of metals. Oxygen grabs electrons from iron

State the terms - blastocoel and blastopore, State the terms - Blastocoel a...

State the terms - Blastocoel and Blastopore? During the development of embryo, single cells divide and form a hollow ball of cells one cell layer thick. Cavity inside this holl

Are there differences between the male and female skeletons, Q. Are there d...

Q. Are there differences between the male and female skeletons? Many general differences exist between female and male skeletons. Male skeleton is normally larger and heavier t

Roots have no chlorophyll and grow in darkness, Roots have no chlorophyll a...

Roots have no chlorophyll and grow in darkness. So how do roots obtain their food? Food made in the leaves is transported to the roots in the phloem of the vascular bundles.

What is biochemical characteristics, What is Biochemical Characteristics? ...

What is Biochemical Characteristics? In the last section we have learnt about the importance of biochemical tests. It must be clear now that biochemical tests are used to -

Explain the treatment of susceptible tb, Treatment of susceptible tb Al...

Treatment of susceptible tb All isolates of Mycobacterium tuberculosis should be tested for antimicrobial susceptibility, but results generally do not become available for at l

Explain avoidance of shock in nutritional care, Explain Avoidance of Shock ...

Explain Avoidance of Shock in Nutritional Care? Loss of plasma proteins lead to decrease in blood volume (hypovolemia) and lowered red blood cell volume causing a potential dan

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd