Clone (verb), Biology

Assignment Help:

Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.

One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.


Related Discussions:- Clone (verb)

How long is the incubation period of the hiv, How long is the incubation pe...

How long is the incubation period of the HIV? What is meant by acute AIDS? The incubation period of the HIV (the time interval among the infection and the beginning of the immu

Lntracardiac hemodynamics, Provides one dimensional ("ice pick") view of...

Provides one dimensional ("ice pick") view of the heart only provides information with respect to the distance of each object from the transducer and no information in the l

What is fluoride inhibitor, Fluoride Inhibitor Fluoride inhibits  enola...

Fluoride Inhibitor Fluoride inhibits  enolase enzyme involved in the conversion of 2-phosphoglerate  to phosphoenol pyruvate.

How different are animal cells from plant cells, How different are animal c...

How different are animal cells from plant cells? Whereas plant cells are eukaryotic, autotrophic, photosynthetic and have chloroplasts and cell wall, the animal cells are eukar

Explain food applications of gum karaya, Explain Food Applications of gum k...

Explain Food Applications of gum karaya The water absorbing and water-holding capacity of Karaya, together with an excellent acid compatibility made it suitable for its use

Hounsfield units of computed tomograpy scan, Hounsfield Units: It is an...

Hounsfield Units: It is an arbitrary scale of gray values for CT. It is an indicator of bone density: - Trabecular bone has a range of 200 - Cortical bone has a range

Define filming projection and technique, Q. Filming Projection and Techniqu...

Q. Filming Projection and Technique? Biplane ventriculography is preferred over single plane ventriculography because it gives more information without additional risk to the p

Define contains permitted synthetic food colour, Normal 0 fa...

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4

Explain neurosyphilis, Neurosyphilis  Symptomatic neurosyphilis, includ...

Neurosyphilis  Symptomatic neurosyphilis, including ophthalmic or otic infection, requires treatment with high doses of aqueous penicillin G IV or procaine penicillin G IM with

What is the rule for the pairing of nitrogen-containing base, What is the r...

What is the rule for the pairing of nitrogen-containing bases in the DNA molecule? And in the RNA? The rule for the pairing of nitrogen-containing bases of the polynucleotide c

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd