Clone (verb), Biology

Assignment Help:

Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.

One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.


Related Discussions:- Clone (verb)

Roots have no chlorophyll and grow in darkness, Roots have no chlorophyll a...

Roots have no chlorophyll and grow in darkness. So how do roots obtain their food? Food made in the leaves is transported to the roots in the phloem of the vascular bundles.

Constituents of food – the major nutrients, Composition of Plant and Animal...

Composition of Plant and Animal Tissues Food supplies nutrients popularly known as major and minor nutrients based on the quantities in which they are required for the living b

Why the peritoneal cavity differ from the normal state, How does the presen...

How does the presence of thick, cloudy, yellowish fluid in the peritoneal cavity differ from the normal state?

Explain in brief about the neuropsychological test batteries, Explain in br...

Explain in brief about the neuropsychological test batteries Neuropsychological tests have the same standardisation requirements as all psychological tests. That is, there is t

Pulmonary artery diastolic pressure, PADP may be determined by measuring th...

PADP may be determined by measuring the end diastolic velocity of PADP = 4 V 2 Pulmonary regurgitant signal and applying the following formula V is  the end diastolic veloci

Sample titration - estimation of vitamin c in chillies, Define Sample Titra...

Define Sample Titration - estimation of vitamin c in chillies? Take a clean and dry large chilli and weigh it. Note the weight of the chilli. Grind the chilli to a fine paste i

Why protein requirements of athletes get increased, Why Protein requirement...

Why Protein requirements of athletes get increased? Protein requirements of athletes, particularly those engaged in strength and power events, may be increased above those of s

Genetics, how does the universality of the genetic code make recombinant DN...

how does the universality of the genetic code make recombinant DNA technology possible

What is symbiotic nitrogen fixation, a) What is symbiotic nitrogen fixatio...

a) What is symbiotic nitrogen fixation? b) Name the two protein components required for this process. Define their role.

Explain wire loop and tube method, Explain Wire loop and Tube Method 1....

Explain Wire loop and Tube Method 1.  Use a 25-gauge dental injection needle, cut to remove beveled end 2. Both ends of-mm diameter steel ligature wire passed through the ne

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd