Clone (verb), Biology

Assignment Help:

Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.

One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.


Related Discussions:- Clone (verb)

Describe how a phagocyte destroys bacteria, Describe how a phagocyte destro...

Describe how a phagocyte destroys bacteria. The phagocyte forms a pouch in its cell membrane and engulfs bacteria in the pouch. It then pinches off the pouch to produce a vesi

Explain the anaesthesia and analgesia, Explain the Anaesthesia and Analgesi...

Explain the Anaesthesia and Analgesia Most implant surgery can be carried out under local anaesthesia, although some patients will require sedation or general anaesthesia. The

What is immune memory, How can an organism that once underwent contact with...

How can an organism that once underwent contact with an antigen be immunized against future infections by the same agent? This phenomenon is known as immune memory . When an a

Define effect of vitamins (a, Define the effect of Vitamins (A, D, K and B-...

Define the effect of Vitamins (A, D, K and B-Complex) on athletes? Vitamins A, D and K have been found to have no ergogenic effects. Ingesting large doses of these vitamins hav

Is dwarf pampas grass invasive, Is dwarf pampas grass invasive? No. the...

Is dwarf pampas grass invasive? No. the root system is shallow and extends only as far as the plant is large. Though if the irrigation system is installed poorly and the pipes

Infectious coryza, I nfectious coryza A highly infectious bacterial di...

I nfectious coryza A highly infectious bacterial disease of chickens caused by Hemop hilus paragallinarum, is characterized by catarrhal inflammation of the upper respirator

How identical proteins form, Are proteins with the same number of each diff...

Are proteins with the same number of each different amino acid that form them necessarily identical proteins? Even if many proteins have the similar number of each dissimilar a

Explain the prokaryotic cells in details, Explain the Prokaryotic Cells in ...

Explain the Prokaryotic Cells in details? Prokaryotic Cells: Cells that lack a membrane-bound nucleus and have very few distinguishable internal structures when observed wit

Introduction of formation of soil, FORMATION OF SOIL Evolution of Eart...

FORMATION OF SOIL Evolution of Earth took place five to eight billion years ago when it was a red hot magma (molten rock). It took millions of years for the magma to cool down

Implantation - pre-embryonic development, Implantation - Pre-Embryonic Deve...

Implantation - Pre-Embryonic Development After entering the uterus and formation of ICM, the blastocyst starts to embed in the endometrium of the uterine wall. By one week aft

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd