Clone (verb), Biology

Assignment Help:

Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.

One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.


Related Discussions:- Clone (verb)

Accuracy of stress echocardiography, Q. Define Accuracy of Stress Echocardi...

Q. Define Accuracy of Stress Echocardiography? The accuracy of stress echo tests for the detection of coronary artery disease is expressed as the sensitivity and specificity of

Energy demand for vigorous or vigorously active lifestyles, Determine Energ...

Determine Energy demand for Vigorous or vigorously active lifestyles? These people engage regularly in strenuous work or in strenuous leisure activities for several hours. Exam

Describe the complete transposition of great arteries, Describe the Complet...

Describe the Complete transposition of great arteries ? Lethal, relatively frequent malformation. Without treatment, it results in 30 per cent mortality within the first week o

Explain the hormonal control of the reproductive system, Explain the hormon...

Explain the hormonal control of the reproductive system in human male. Define totipotency. Verify the two different routes of regenerating plantlets from callus culture.

What is the ploidy of these gamete-forming cells, What is the name of the c...

What is the name of the cells capable of making gametes? What is the ploidy of these gamete-forming cells? The cells that form gametes are the germ cells as opposed to the soma

Explain selective and differential culture media, Explain Selective and Dif...

Explain Selective and Differential Culture Media? These are used for isolation of specific organisms. These are designed to isolate a group of closely related organisms and dif

Define blood pressure, Blood Pressure Blood pressure may be defined as ...

Blood Pressure Blood pressure may be defined as the force or pressure which the blood exerts on the walls of the artery. Blood pressure varies with the age of the individual. B

Phosphorus cycle, Phosphorus cycle is sedimentary cycle because it does not...

Phosphorus cycle is sedimentary cycle because it does not enter into atmosphere. It can be found on earth in water, soil and sediments. The phosphorus cycle is the biogeochemical c

Explain long-term risk estimation, Explain Long-term Risk Estimation ? ...

Explain Long-term Risk Estimation ? Many studies have examined long-term risks associated with risk factors using traditional epidemiological methods. Framingha investigators

Fate of pyruvate, Pyruvate Pyruvate has three different  fates. Under a...

Pyruvate Pyruvate has three different  fates. Under aerobic conditions,  pyruvate enters mitochondriaand is converted to acetyl CoA. The acetyl CoA enters thecitric acid cycle.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd