Clone (verb), Biology

Assignment Help:

Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.

One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.


Related Discussions:- Clone (verb)

Explain the glycemic index in chronic diseases, Explain the Glycemic index ...

Explain the Glycemic index in Chronic Diseases? In addition to serve as an aid in planning diets for diabetics, GI of diets has been linked to a number of chronic diseases. Sev

Name the nitrogenous waste excreted in the larval, What benefit does the se...

What benefit does the sea anemone get in the sea anemone-hermit crab facultative mutualism? Give an alternative term for this part of mutualism. Name the nitrogenous wast

Basic working of midbrain, Q. Basic working of Midbrain? Midbrain: Also...

Q. Basic working of Midbrain? Midbrain: Also known as the mesencephalon, this part is located behind the frontal lobes and in the center of the entire brain. It deals with func

Working of eye, WORKIN G OF EYE - Human eye is sensitive to light wave...

WORKIN G OF EYE - Human eye is sensitive to light wavelenght ranging from 380 - 760 nm. Bees, ants, spiders and gold fish can see uttraviolet light which is invisible to hu

Oligosaccharides, OLIGOSACCHARIDES They are small-sized polymers of ...

OLIGOSACCHARIDES They are small-sized polymers of monosaccharides having 2 - 6 simple sugars, occasionally upto 9 - 10. Oligosaccharides are soluble in water, sweet to t

Determine the name of material used for bcc, Determine the name of Material...

Determine the name of Material used for BCC You must be interested to know about type of material you can prepare and some material is available in hospitals and clinics for us

Explain regulation of glycolysis, Regulation of Glycolysis There are th...

Regulation of Glycolysis There are three markedly exergonic reactions in  the glycolytic pathway, which  are considered physiologically irreversible. These reactions are cataly

An a=t mispairing leads to an a=c substitution, An A=T mispairing leads to ...

An A=T mispairing leads to an A=C substitution. The other DNA helix will contain a(n) __ pair. a. A=C b. A=T c. G=C d. B=Q e. T=T Can you also explain it please so if I'm ask

Zoology, General characters of phylum protozoa

General characters of phylum protozoa

What is the form that produces gametes, In the metagenesis of Aurelia and O...

In the metagenesis of Aurelia and Obelia what is the form that produces gametes? What is the form that reproduces asexually? In the metagenesis of some coelenterates, like Obel

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd