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Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.
One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.
Caseous lymphadenitis Caseous lymphadenitis is a chronic disease of sheep and goats. It is characterized by abscess formation in lymph nodes and sometimes in lungs and other organ
What is the difference between spermatogonium and spermatocyte I? The male germ cells are the spermatogonia (diploid cells, 2n) located in the testicles. They mature and by mea
Single chromosome of a pair is known as homologue.In Homologous pair two identical chromosomes are there. Each one chromosome in a Homologous pair is known as Homologue.
Congenital impotentia genarandi Some bulls which are otherwise normal and healthy produce semen containing spermatozoa with specific morphological abnormalities together with
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Effect of processing on mineral content of foods Minerals are comparatively stable under processing conditions such as heat, light, use of oxidizing agents and extremes in pH.
CARDINAL MANIFESTATION OF CARDIOVASCULAR DISORDERS Cardiovascular symptoms that most often trouble patients are: 1. Chest Pain 2. Shortness of Breath 3. Palpitation
Explain about the PH - Nutritional Biochemistry? You have already studied about pH in your undergraduate years and know what it means. Yes, mathematically we can define pH as e
What happens to the TSH (thyroid-stimulating hormone) blood level in hypothyroidism? Why is there enlargement of the thyroid in the endemic goiter disease? When there is low T3
What Do We Mean By Culture Media? Culture media, you would realize, is a solid or liquid preparation containing all the nutrients required by microbes for growth. So then, what
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