Clone (verb), Biology

Assignment Help:

Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.

One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.


Related Discussions:- Clone (verb)

Disaster management cycle and it''s components, Disaster management is aime...

Disaster management is aimed at minimizing or avoiding the potential losses from extreme events and assuring rapid and proper help to the affected people and ascertaining a quick a

Define the term- ultrafiltration, Define the term- Ultrafiltration Ultr...

Define the term- Ultrafiltration Ultrafiltration occurs when the flow of a substance across a cell membrane is increased by a hydrostatic pressure. This process was initially b

Explain sexually-transmitted diseases, Sexually-transmitted diseases M...

Sexually-transmitted diseases Many travelers have new sexual contacts during their journeys and are at increased risk for acquiring sexually transmitted diseases including HIV

How much ampicillin can dissolve in 400 ml, How much ampicillin (sodium sal...

How much ampicillin (sodium sal, mw=371.40) would you dissolve in 400 mL of water to make 80 mg/ml solution of ampicillin?

Why dairy or milk product are important for human body, Why Dairy or milk p...

Why Dairy or milk product are important for human body? This first food of mammals is rich in body-building proteins and bone-forming calcium, besides being the only source of

What is the economic importance of sponges, Q. What is the economic importa...

Q. What is the economic importance of sponges? a few chemical substances secreted by sponges have anti-inflammatory, anti-tumoral activities and antibiotic and they are used in

Define the micronutrient deficiencies, Define the Micronutrient Deficiencie...

Define the Micronutrient Deficiencies? In the previous section, we learnt about macronutrient deficiencies i.e. protein energy malnutrition in children. We are now aware that t

What carbohydrate characteristic chemical features include, Carbohydrate ch...

Carbohydrate characteristic chemical features include: Select one: a. the potential to form multiple hydrogen bonds. b. the existence of one or more asymmetric centers.

What is secretary diarrhoea, Q. What is Secretary diarrhoea? Secretary ...

Q. What is Secretary diarrhoea? Secretary diarrhoea: It is a result of active secretion OF electrolytes and water by the intestinal epithelium caused by bacterial and viral inf

Diagnosis and treatment of myocarditis, Diagnosis X-ray chest show...

Diagnosis X-ray chest shows cardiomegaly.  ECG shows conduction defect, low voltage QRS, atrial and ventricular disrhythrnias.  Echocardiogram - decreased LV cont

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd