Clone (verb), Biology

Assignment Help:

Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.

One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.


Related Discussions:- Clone (verb)

What is fern allies - equisetum, What is Fern Allies - Equisetum ? The ...

What is Fern Allies - Equisetum ? The earliest land plants are thought to have evolved from green algal ancestors, since they share many features. Both green algae and plants h

What is mechanical digestion, What is mechanical digestion? In molluscs, ar...

What is mechanical digestion? In molluscs, arthropods, earthworms, birds and vertebrates, in general, which organs respectively participate in this type of digestion? Mechanica

State the term - antecedent and consequence control, Antecedent and consequ...

Antecedent and consequence control Therapy and consultation cannot be effective unless the behaviours to be changed are understood within a particular context. The process of u

Agro industrial-incriminating factors in feeds, Agro Industrial-Incriminati...

Agro Industrial-Incriminating factors in feeds There are many anti-nutritional factors present in feeds and fodders which affect the utilization of nutrients. Some of these are

Female reproductive system - fallopian tube, F ALLOPIAN TUBE - Ferti...

F ALLOPIAN TUBE - Fertilisation occur in it. 10-12 cm long muscular tube. It is supported by double fold of peritonium. It shows 4 regions - (i ) Infundibulum - Bro

Artificial insemination, ARTIFICIAL INSEMINATION - Artificial insemi...

ARTIFICIAL INSEMINATION - Artificial insemination is a technique to make a female pregnant by artificially introducing semen into the vagina. Artificial insemination has

Neuston - aquatic ecosystem, Neuston - Aquatic Ecosystem These are una...

Neuston - Aquatic Ecosystem These are unattached organisms which live at the air-water interface such as floating plants and several types of animals. Some spend most of their

Type of membrane is the cell membrane, Q. Concerning permeability what type...

Q. Concerning permeability what type of membrane is the cell membrane? The cell membrane is a selectively permeable membrane that is it allows the passage of water and some sel

What is a pipette and how is it used, What is a pipette and how is it used?...

What is a pipette and how is it used? There are lots of types of pipettes (or pipette), but most are basically a fancier version of a medicine dropper or eye dropper. They are

Measurement of functional property of fats and oil, Measurement of Function...

Measurement of Functional Property of fats and oil Fats and oils have several functional properties that affect the quality of processed foods. In deep fat frying, the roles p

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd