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Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.
One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.
What are the cells that produce the myelin sheath? Of which substance is the myelin sheath formed? In the central nervous system (CNS) the myelin sheath is made by apposition o
Q. Explain about Dietary Management? Treatment for gout often include a diet of lower purine intake. Indeed, about one third of the body's uric acid can be attributed to diet.
Morphogenetic Processes We discussed earlier the process through which a fertilized egg assumes the shape of a ball or a sheet of cells around a cavity, called blastula. Cons
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Viscosity Viscosity, as you may already know, is associated with fluid flow. It is the internal friction which tends to bring to rest portions of the fluids moving relative to
When William was helping victims following a devastating earthquake in a region that was not prepared to swiftly set up adequate temporary shelter, he developed severe diarrhea. He
How to Calculate the Requirements for the Calcium in Body? Requirements for calcium depend upon the rate at which calcium is incorporated into bone; they are therefore highest
Q. Periapical radiography - criteria for endosteal implants? Periapical radiographs are images of a limited region of the mandibular or maxillary alveolus. Periapical radiogra
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