Clone (verb), Biology

Assignment Help:

Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.

One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.


Related Discussions:- Clone (verb)

Types of glials cells and their functions, Types of Glials Cells and their ...

Types of Glials Cells and their Functions In the nervous tissue, besides the nerve cells there is one other cell type -the neuroglia which is present in large numbers and occu

Wound botulism, Q. Wound Botulism? It is the rarest form of botulism. T...

Q. Wound Botulism? It is the rarest form of botulism. The illness results when C. botulinum by itself or with other microorganisms infects a wound and produces toxins, which re

Explain the periapical surgery - endodontic surgery, Explain the Periapical...

Explain the Periapical surgery - Endodontic Surgery a) Curretage 1 b) Root-end ressection 2 c) Root-end preparation 3 d) Root-end filling 4

Conjugated proteins, CONJUG A TE D PROTEINS Besides being made of po...

CONJUG A TE D PROTEINS Besides being made of polypeptides, the conjugated proteins posses additional groups, metals or ions. Consists of protein and non-protein parts.

Difference between that is alive, Is there a difference between something t...

Is there a difference between something that is alive and something that is a living organism?

Explain nutrient cycling and their processes, Explain Nutrient Cycling and ...

Explain Nutrient Cycling and their processes? Two major processes are common to all ecosystems: nutrient cycling and the flow of energy. These two functions are carried out by

What are the types of plant geotropisms, What are the types of plant geotro...

What are the types of plant geotropisms? Why do the stem and the root present opposite geotropisms? The parts of geotropisms are the positive geotropism, that in which the plan

Coeleterates, what are the characteristics of cleavage formed in coelentera...

what are the characteristics of cleavage formed in coelenterates?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd