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Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.
One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.
Name the methods for extracting phosphorous Phosphorus/phosphate - Phosphorous is estimated as phosphate. Some methods for extracting phosphorous are as follows: Dyer met
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What is homogenization Emulsions may also be stabilized by a process known as homogenization, in which the size of the dispersed fat globules is greatly reduced to more or less
Determine Major Aspects of the Mechanism of Freeze Drying? The major aspects of the mechanism of freeze drying are: (a) The removal of vapour from the subliming ice front w
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