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Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.
One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.
Explain Zipping of the Apical Canal a. If the preparation is continued with progressively larger and stiffer file lead to : zipping perforation or tear apical foramen.
The Intine Layer As soon as the tetrads release the microspores, the inner layer of the pollen wall (intine) is formed. Proteinaceous lamellae are embedded in the matrix of th
Which of the following statements is correct? A. Hemoglobin binds O2 more tightly at pH 7.2 than at pH 7.4. B. Fetal hemoglobin under physiological conditions binds O2 more t
what is the habitat of spongilla
What is Oxidation and Reduction? Oxidation and Reduction : Most people are familiar with oxidation as it pertains to the rusting of metals. Oxygen grabs electrons from iron
Q. What is the nitrogen waste in amphibian larvae and in the adult animal? The terrestrial adult excretes urea since amphibian larvae are aquatic they excrete ammonia.
Implant insertion The technique for insertion of the implant depends largely upon the system being used. In general, the final bone preparation site diameter is slightly smalle
What is the excretory organ of an agama lizard?
Q. Which are the beings that form the kingdom Fungi? The kingdom Fungi is created by fungi. Q. Which are the beings that form the kingdom Plantae? Are algae parts of this k
EXCRETIO N IN COCKROACH- Animal is uricotelic. Excretory organs are malphighian tubules. 60-150 in 6-8 groups. In each group 10-15. Yellow coloured, blind tube, open in gut
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