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Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.
One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.
What are the main negative ions found in living beings? The major anions found in living beings are the chlorine anion (Cl-), the phosphate anion (PO4--), the bicarbonate anion
Resistance Acyclovir-resistant HSV occurs mainly in immunocom- promised patients treated with the drug; isolates are usually also resistant to valacyclovir and famciclovir. Re
who proposed trinomial system of classification? in ALLEN modules ans is Huxley & Stricklandt but in AAKASH its given Lamarck so what is correct?
Under conditions when a cell only needs to use the nonoxidative portion of the Pentose Phosphate pathway to synthesize ribose-5-phosphate, the elevated levels of NADPH in the cell
What is an accommodation? Accommodation is the ability of the eye to focus on an object situated at variable distances from the eye. Like a camera, even the eye requires to cha
Q. Explain Ciguatera Fish Poisoning? Ciguatera probably represents the most common cause of poisoning from fish in tropics. Ciguatera comprises over 50% of all reported case
Risk management option assessment : Identification of available management options Selection of preferred management option , including consideration of an appropriate saf
Vibrio parahaemolyticus gastroenteritis While most other known food poisoning syndromes may be contracted from a variety of foods, V. parahaemolyticus gastroenteritis is contr
What is meant by Gene Expression? The synthesis of protein under the influence of gene is called Gene Expression. All human cells are derived from a single cell, fertilized egg
Adenosine triphosphate (ATP) is a general form in which the energy is stored in living systems; comprises of a nucleotide (with ribose sugar) with three phosphate groups. Energy c
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