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Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.
One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.
What is phellogen? What is its function? Phellogen, also called as cork cambium, is the meristematic plant tissue responsible for the formation of the periderm (the covering of
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ISOZYMES : an enzyme having different molecular forms but catalyzing the same reaction.
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how do i prove in a diagram that water is needed for photosynthesis
STATE THE ECONOMIC AND ECOLOGICAL IMPORTANCE OF ECHINODERMS
Define the Symptoms of Dry beriberi? Early symptoms are similar to those found in wet beriberi. The muscles become progressively wasted and weak and walking becomes difficult.
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