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Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.
One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.
An ecosystem is a self sufficient interacting system in the biosphere. Eg. forest, desert, pond etc. almost, all the ecosystem have more or less similar fundamental plan for their
briefly explain filter feeding in holozoicanimals
Explain the chemical properties of milk Autoclaving milk, wherein temperature of around 121 o C is achieved, causes browning. The brown colour is due to the heat effecting an i
Slight or no Metamorphosis In primitive wingless insects (Apterygota) such as spring tails, silver fish etc. and in secondarily apterous insects the young ones which hatch fr
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related words for reproductive isolation.
Where do the photochemical and the chemical stages of photosynthesis occur? The photochemical stage of the photosynthesis process happens mainly on the thylakoids (the green pa
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Q. What are the Signs of cardiac tamponade? The clinical presentation will be that of a low output state with anxiety, restlessness, dyspnoea, sweating, cold extremities and dr
Agro-industrial byproducts Agro-industrial by-products in Southeast Asia are available in plenty, due to the emphasis on crop cultivation. By-products of agro-industries repre
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