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Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.
One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.
What is bicuspid aortic? Bicuspid aortic valve is the most common form of CHD seen in adults. Isolated bicuspid aortic valves may be stenotic or non-stenotic to start with. T
Photosynthesis rate varies according to the photic energy intensity. Do the similar occur in aerobic respiration? What happens to the glucose balance as a result of these variation
Evaluation This is the last step in nursing process. Throughout care, you constantly evaluate the progress the client has made towards reaching the pre-established goals/ ob
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Use of repellents Avoidance and early removal of ticks are the first steps in prevention of Lyme disease. In highly endemic areas, when an engorged Ixodes scapularis tick is at
Q. Define a closed circulatory system? A closed circulatory system is one in which blood circulates only inside blood vessels for this reason the blood pressure is higher in so
Dietetic Foods for diabetes: With advances in food technology, foods for special use by diabetics are now available in the global market, they are emerging in India also. These
Q. How is the chromosome region where the centromere is located called, and how chromosomes classified in the relation to the position of their centromere? The chromosome regio
Q. How many ATP molecules are made after the aerobic respiration and what is the net energetic gain of the process? After aerobic respiration 38 ATP molecules are made with the
Q How do antagonistic mechanisms manage homeostatic regulation? The homeostatic maintenance of the body typically occurs by means of alternating antagonistic compensatory mecha
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