Clone (verb), Biology

Assignment Help:

Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.

One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.


Related Discussions:- Clone (verb)

What are the main human diseases caused by fungi, Q. What are the main huma...

Q. What are the main human diseases caused by fungi? The major human diseases caused by fungi in immunocompetent patients are , blastomycosis, histoplasmosis, paracoccidioidomy

Food-borne zoonoses, Food-borne zoonoses Food-borne illnesses are seri...

Food-borne zoonoses Food-borne illnesses are serious public health problem. They are associated with the ingestion of contaminated foods by microorganisms and their toxins, ma

Animals - slow moving waters, Animals - Slow Moving Waters Zooplankton...

Animals - Slow Moving Waters Zooplankton are common here and include an assemblage of protozoa and smaller crustacean, such as water flies, and copepods. Neuston occurring her

Palaeobiology, Palaeobiology : This deals with the study of fossils and rem...

Palaeobiology : This deals with the study of fossils and remains an impressions of the past organisms found in the rock of different ages. Paleobiology is a growing or comparativel

Formation and role of hypoblast, Formation and Role of Hypoblast Hypo...

Formation and Role of Hypoblast Hypoblast is made as a result of two processes. First, some cells individually leave the blastoderm and move down via poly invagination into t

Concept mapping, Mrs. Jackson is a 76 year old woman living in a skilled nu...

Mrs. Jackson is a 76 year old woman living in a skilled nursing facility. She has Hx: CVA with left-sided weakness, incontinent of bowel and bladder, PEG tube, GERD, HTN, Dementia,

What is transportation of sick newborn infants-heart disease, What is Trans...

What is Transportation of Sick Newborn Infants with Heart Disease Communication : The decision to transport a newborn to a tertiay referral centre with facilities for specia

Fungi, what is primitive fungi?

what is primitive fungi?

Why iron is required as a part of haemoglobin, Why Iron is required as a pa...

Why Iron is required as a part of haemoglobin? As a part of haemoglobin, iron is required for the transport of oxygen, to all cells in the body. Thus, haemoglobin is critical f

How many different gene types are possible, In lab rats, hair color and tex...

In lab rats, hair color and texture are controlled by two genes, the A gene for color and the B gene for texture. in both of these genes, the alleles show no dominance to each othe

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd