Clone (verb), Biology

Assignment Help:

Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.

One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.


Related Discussions:- Clone (verb)

What is meant by the arch reflex, What is meant by the arch reflex? In ...

What is meant by the arch reflex? In some situations the movement of the skeletal striated muscles does not depend upon commands of the superior motor neurons, i.e., it is not

Classification, what is the mode of nutrition in fungi?

what is the mode of nutrition in fungi?

Illustrate basic working of pineal gland, Q. What is the pineal gland? ...

Q. What is the pineal gland? The pineal gland also known as epiphysis or pineal body is situated centrally in the head. It secretes the hormone melatonin. A hormone produced at

Toxic agents present in which food, Toxic agents present in food which inte...

Toxic agents present in food which interfere with thyroxine synthesis lead to the development of: 1. toxic goitre 2. cretinism 3. simple goitre 4. thyrotoxicosis si

Define regulation of malice enzyme by starvation & refeeding, Define regula...

Define regulation of malice enzyme by starvation & refeeding? ME catalyzes the oxidative decarboxylation of malate to pyruvate and CO 2 simultaneously generating the reduced

Mold, Does mold have roots? When mold is inside on wood - are there roots t...

Does mold have roots? When mold is inside on wood - are there roots that go into substrate? I am trying to find this out today if possible to evaluate a remediator''s plan. I am wo

Describe the formation of fibrous tissue where it not exist, Which of the b...

Which of the below terms is used to describe the formation of fibrous tissue where it generally does not exist? Is it: a) Fibrosis b) Fibrogenesis c) Fibrositis d) Non

How much calories in dried fruits, How much calories in Dried fruits Dr...

How much calories in Dried fruits Dried fruits are exceptionally rich in calories and they supply significant amounts of minerals. Of the vitamins found in fruits, β-Carotene a

Evolution of photosynthesis and aerobic respiration, Evolution of Photosynt...

Evolution of Photosynthesis and Aerobic Respiration It is assumed that the earlier form of the bacteria utilised H 2 S for the preparation of food. The following reaction shows

DNA REPAIR, assignment for discussing DNA REPAIR

assignment for discussing DNA REPAIR

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd