Clone (verb), Biology

Assignment Help:

Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.

One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.


Related Discussions:- Clone (verb)

Describe coping skills, Have you ever said, ‘I cannot cope up with this?' O...

Have you ever said, ‘I cannot cope up with this?' Or have you heard any one who is sick in your family saying, ‘I cannot cope with my sickness' or ‘I can not take care of this sick

Define about yersiniosis, Define about Yersiniosis In the genus Yersini...

Define about Yersiniosis In the genus Yersinia, 11 species are recognized, including Y. pestis, the cause of plague. The species of primary interest in foods is Y. enterolytica

Intervention strategy, Intervention Strategy There are five basic ways...

Intervention Strategy There are five basic ways in which government can correct market failures and ensure equity: inform, regulate, mandate, finance and deliver health care s

What are cnidocytes, What are cnidocytes? What is the name of the capsule i...

What are cnidocytes? What is the name of the capsule inside the cnidocyte? What are the biological functions of this structure? Cnidocytes are specialized cells present in coel

What is cyclosis, Cyclosis is a type of internal cell movement in which an ...

Cyclosis is a type of internal cell movement in which an oriented flow of circulating material is formed and maintained in the cytoplasm by the action of microfilaments. Cyclosis i

What are vitamins, Q. What are vitamins? What are the major vitamins needed...

Q. What are vitamins? What are the major vitamins needed by humans? Most vitamins are coenzymes fundamental substances for the enzyme functioning that are not produced by the o

What is the binding between two amino acids, Q. What is the binding between...

Q. What is the binding between two amino acids called? The chemical bond between two amino acids is called a peptide bond.

Explain the treatment of iron deficiency anaemia, Explain the Treatment of ...

Explain the Treatment of iron deficiency anaemia? Oral iron is the preferred method of treatment of IDA. The dosage is decided depending on the severity of the condition. Gener

Before purchasing - nursing software, Before Purchasing  Ideally it...

Before Purchasing  Ideally it's wise to buy a readymade software with options to further customize it to suit your managerial functions.   A totally Custom-made softwar

State the term - early therapeutic intervention, State the term - Early the...

State the term - Early therapeutic Intervention Since outcome may be ameliorated by well timed and appropriate treatment, the prevailing belief is that dysfunction should be id

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd