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Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.
One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.
There has not been rain for several months. A biologist is driving down the highway and notices that a large number of corn fields have corn that is wilted and short. She wonders i
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At the present level of the biotechnology what are the main techniques of genetic engineering? The major techniques of genetic engineering today are: the recombinant DNA techno
Results of CABG: In most centres where this operation is routinely conducted the mortality is 1-2 per cent. In high-risk group of patients this may go up to 5 to 10 per cent.
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Categories of Gifted Species This group of nitrogen fixers has been divided into three categories: Rhizobium which includes fast growing species, Bradyrhizobium which
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Q. What is the germ layer from which the coeloms originate? The coeloms are deriving from mesoderm.
What is the best microscope to get a detailed view of the parts inside of a preserved plant cell
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