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Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.
One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.
What is antistaphylococcal prophylaxis and pseudomonas aeruginosa? I''m doing a project on cystic fibrosis, and I''m curious what these terms have to do with the disease. Thanks!
BLOO D PRESSURE Is the result of the sum of (i) Osmotic colloidal pressure of blood (ii) Elastic recoil of blood vessel's wall.
Dry Stigma - Category of Stigma The Cotton (Gossypium hirsutum) stigma is covered with long unicellular hairs. At the time of pollination, the stigmatic hairs show a distinct
During a fever in a human, A. shivering can happen when the actual body temperature is higher than the set point for body temperature during the fever. B. the control system
Q. Explain Atrial Pressure? The RA pressure wave form has three positive deflections - "a", "c", and "v" waves. The "a" wave is due to atrial systole and follows the P-wave on
Define ailing and failing Clinically unhealthy implants are classified as "ailing" or "failing". It is necessary to distinguish between an ailing versus a failing implant to de
Define Factors influencing uptake from lumen to intestinal cells? inhibition by intrinsic matrix, inhibition by dietary fiber sources, differential crowding by
HISTORICAL DEVELOPMENT OF PSYCHIATRIC NURSING: History is a record of opportunities, recognized and informed, that produce profound changes in a given field. History of psyc
Reduction of NADP+ occurs during what?
Rituals: The social life of the earliest human groups or tribes revolved around food gathering. To begin with, they must have collected anything they could eat-seeds, nuts, f
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