Clone (verb), Biology

Assignment Help:

Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.

One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.


Related Discussions:- Clone (verb)

Nature of viral diseases, N a ture of viral diseases Viral diseases ...

N a ture of viral diseases Viral diseases are manifested in acute, sub-acute or chronic forms, as frank clinical cases or as latent infections, some of which are fatal. Thes

Basis of classification on form-relationship principle, Q. Basis of Classif...

Q. Basis of Classification on Form-relationship principle? As pointed out earlier, this system is mainly based on Form-relationship principle. thus the various genera were. g

Define the insulin resistance - obesity, Define the Insulin resistance - Ob...

Define the Insulin resistance - Obesity? Insulin resistance is a condition in which your body cells cannot utilize insulin efficiently although sufficient amounts are secreted

Monohybrid cross, why does the monohybrid always result in a 1:2:1 ratio?

why does the monohybrid always result in a 1:2:1 ratio?

Pathogenesis of viruses, Pathogenesis of Viruses Viral diseases An...

Pathogenesis of Viruses Viral diseases An indicated before, viruses cause a number of infectious human diseases from minor ailments like common cold, to fatal disease, lik

Disadvantages of m - mode echocardiography, Various structures are seen in...

Various structures are seen in two-dimensional planes; hence mental conceptualization is still required because the heart is three-dimensional. Now, newer technology has evolved

Gastroesophageal reflux, Q. Show Gastroesophageal reflux? Obesity is th...

Q. Show Gastroesophageal reflux? Obesity is thought to be another potential predisposing factor to gastroesophageal reflux or GERD. Maintenance of ideal weight for age may help

What is the function of lymph nodes, Explain function of lymph nodes? L...

Explain function of lymph nodes? Lymph nodes have white blood cells which ingest bacteria and prevent them from reaching the circulation.

Determine the function of monomeric enzymes, Determine the fuction of Monom...

Determine the fuction of Monomeric enzymes Monomeric enzymes include, pepsin, like the pancreatic serine proteases, plays a role in the digestion of proteins eaten by mammals.

What are the grain boundaries, What are the Grain boundaries Grain boun...

What are the Grain boundaries Grain boundaries disrupt the motion of dislocations by a material, so reducing crystallite size is a common way to improve strength. Since grain b

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd