Clone (verb), Biology

Assignment Help:

Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.

One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.


Related Discussions:- Clone (verb)

Define association between inflammation and fever, Q. What is the associati...

Q. What is the association between inflammation and fever? In the tissue region where inflammation occurs prostaglandins, cytokines, bacterial toxins, interleukins and endothel

What a test tube brush and how is it used, What a test tube brush and how i...

What a test tube brush and how is it used? It is a method, made with nylon bristles attached to a twisted-wire shaft, used to knock the bottoms out of test tubes.

Describe the origin of muscle cramps and pains, Q. How can the knowledge ab...

Q. How can the knowledge about fermentation describe the origin of muscle cramps and pains after intense physical exertion? A typical fermentation process due to oxygen scarcit

.ecology, describe between native and non-native species from the Otay rive...

describe between native and non-native species from the Otay river valley

Chemiosmosis, Chemiosmosis is the process by which the ATP is produced in ...

Chemiosmosis is the process by which the ATP is produced in inner membrane of a mitochondrion. It is the electron transport system transfers protons from the inner compartment to

Explain microbiology of soil, Q. Explain microbiology of Soil? Ans. ...

Q. Explain microbiology of Soil? Ans. You would realize that the soil contains greatest varieties of microorganisms. It actually serves as a medium for growth and developm

Vasa diffrentia, VAS A DIFFRENTIA - Originate from wollfian duct ....

VAS A DIFFRENTIA - Originate from wollfian duct . It comes out fo cauda epdidymis enters into abdominal cavity through inguinal canal, forms loop round the uretor and j

Cell physiology, why is epidermis peeledsuggest why a marine amoeba does no...

why is epidermis peeledsuggest why a marine amoeba does not have a contractile vacuole

Introduction to ecosystem, The terms ecosystem was introduction by Sir Arth...

The terms ecosystem was introduction by Sir Arthur tensely in 1935. An ecosystem can be defined s " a structural and function unit of biosphere consisting of living organisms and t

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd