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Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.
One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.
Atmosphere is a multilayer blanket of various gases such as N2 (78.80%), O2 (20.95%), Ar(0.93%), CO2 (0.03%), water vapor and some other gases. This envelope extends from earth's c
CONDUCTIO N OF IMPULSE - IN NON-MYELINATED AXONS - The impulse moves along the axon as local effect by altering the permeability of neighbouring Na + channels and af
OBJECTION OF CELL THEORY (REASONS OF DISCARTION) (i) Living organism are composed of various types of cells and their product but members of monera & protista don't have
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Q. Whcih group of individual animals and plants called Species? A group of individual plants and animals that are fundamentally alike is treated as a ,species. Species is a par
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What are some examples of interspecific competition? Examples of interspecific competition are: the dispute between vultures, worms, flies and microorganisms for carrion and th
Evolutionary relationship among various animal groups is depicted in the cladogram given below. P, Q and R respectively shown: a. Vertebrae, hair and four limbs. b. B
How many different types of gametes can be formed by individuals of the following genotypes? What are they in each case a) AaBb b) AaBB c) AaBbCc d) AaBBCc e) AaBbcc f) AaBbCcDdEe
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