Clone (verb), Biology

Assignment Help:

Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.

One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.


Related Discussions:- Clone (verb)

Optic vesicle, Optic vesicle We have explained earlier that the presum...

Optic vesicle We have explained earlier that the presumptive material for the optic vesicles lies in qe eye field in the anterior region of the early neural plate. Experiments

Biological species concept, Is the biological species concept subjective or...

Is the biological species concept subjective or objective? How about the phylogenetic species concept or is it subjective or objective?

List the five major phases of the cell cycle, List the five major phases of...

List the five major phases of the cell cycle, and briefly explain what happens during each phase. G1 phase: the cell grows. S phase: DNA is copied. G2 phase: the cell prepares

The movements of the foetus in which month of pregnancy, The first movement...

The first movements of the foetus and appearance of hair on its head are usually observed during which month of pregnancy? 1. Fourth month 2. Fifth month 3. Sixth month

Define diagnostic criteria for bulimia nervosa, Define Diagnostic Criteria ...

Define Diagnostic Criteria for Bulimia Nervosa? 1. Recurrent episodes of binge eating. An episode of binge eating is characterized by both of the following a) Eating, in a d

Types of insulin, There are different types of insulin available and prescr...

There are different types of insulin available and prescribed by the doctor according to the need of the patient. Insulin is grouped according to the speed of action in the body i.

What is ribosomes , Each  ribosome  having  of two  subunits  a sma...

Each  ribosome  having  of two  subunits  a small  subunit  and  a large  subunit, every of that is a multi component  complex  of rRNAs (ribosomal  RNAs)  and ribosomal proteins t

Classification of drugs, CLASSIFICATION OF GENERAL DRUGS - 1 .       ...

CLASSIFICATION OF GENERAL DRUGS - 1 .       NATURAL DRUGS - Obtained from natural sources as minerals, animals, plants & microorganism. (i)       Minerals - It include

What are the antigen-presenting cells of the immune system, What are the an...

What are the antigen-presenting cells of the immune system? The antigen-presenting cells of the immune system, also called as APC cells, are cells that do phagocytosis and dige

Illustrate food applications of cmc, Food applications of CMC In common...

Food applications of CMC In common with other linear water-soluble polymers, cellulose gum solutions are pseudoplastic that is the viscosity decreases as the  rate of shear inc

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd