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Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.
One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.
A lk a l in e indigestion The disease occurs due to the consumption of excessive amount of protein rich concentrate or non protein nitrogenous substance like urea, and is
DIVERSITY OF AMPHIBIANS ASSIGNMET IN BIOLOGY PLWASE HELP ME TO DO THIS ASSIGNMENT AND I CAN GET GOOD MARKS IN THIS ASSIGNMENT SO GIVE ME TNE ASSIFNMENT OF THIS TOPIC DIVERSITY OF A
Q. Vitamins requirement in dyslipidemia? Antioxidants and flavonoids, natural vitamin E, vitamins C and Aare nutrients (vitamins) that scavenge cell-damaging free radicals and
Explain Micafungin sodium Micafungin sodium (Mycamine - Astellas), the second echinocandin antifungal1 to become available in the US, has been approved by the FDA for intraveno
The process by which gametes are produced in the gonads is known as gametogenesis. The process of formation of male gametes or sperm is known as spermatogenesis is of great sign
EXCRETION IN PRAWN- A pair of antennary gland or green gland and a median renal sac present. Green glands lie at the base of antennae & open out by renal aperture.
How did Pasteur's experiment answer the objections raised by supporters of the "vital force" hypothesis? Pasteur's experiment allowed air from the out- side to mix with air fro
A laboratory mouse homozygous for an RFLP marker is mated to a wild mouse that is heterozygous for that marker. One of the heterozygous individuals resulting from this cross is mat
What are the trends of the gametophyte in the evolution of plants? A tendency of the gametophyte evolution in plants has been towards the formation of gametes that are independ
Describe the Plasma Homocysteine levels ? Several studies have investigated the contribution of homocysteine to CVD risk both among immigrant Indians and those living in India.
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