Clone (verb), Biology

Assignment Help:

Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.

One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.


Related Discussions:- Clone (verb)

Proteases of animals, Proteases of animals From the table you can see ...

Proteases of animals From the table you can see that these exopeptidases and endopeptidases attack specific peptide bonds depending on the chemical group near them. The inacti

Stereochemistry of amino acids - optical properties, Stereochemistry of Ami...

Stereochemistry of Amino Acids: Optical Properties All  amino acids (except glycine) rotate the plane of polarized light because of  the presence of an asymmetric center at C-2

Explains the definition of semi-conservative, Meselson and Stahl demonstrat...

Meselson and Stahl demonstrated that DNA replication is a semi-conservative process. Which of the following best explains the definition of "semi-conservative"? A. The two stra

Homework, 1) a) Initially, how do skeletal muscles (the type of muscle atta...

1) a) Initially, how do skeletal muscles (the type of muscle attached to bones) make ATP? b) write the reaction for this procss (Crp +ADP => Cr + ATP) as a ''coupled'' reaction, id

Determinants of health status, Determinants of Health Status The linkage...

Determinants of Health Status The linkage between economic development and health was discussed in the previous unit. The present unit deals with an identification of the factor

Name the animals having a single opening as mouth and anus, One example of ...

One example of animals having a single opening to the outside that serves both as mouth as well as anus is: 1. Octopus 2. Asterias 3. Ascidia 4. Fasciola Ascidia i

Determine the statement- the evolution of color vision, Based on your readi...

Based on your reading of the article entitled "The Evolution of Color Vision", which of the following is a false statement? A. Trichomatic vision is found in all male and femal

Phylum ciliophora - protozoan, Phylum Ciliophora - Protozoan Simple ci...

Phylum Ciliophora - Protozoan Simple cilia or compound ciliary organelles typical in at least one stage of life cycle; subpellicular cilia present even if surface cilia are ab

Vital dye staining, Vital Dye Staining In this type of method, a piec...

Vital Dye Staining In this type of method, a piece of agar or cellophane soaked in the solution of a vital dye (Neutral Red, Nile Blue Sulphate, and Bismarck Brown etc.) is a

Crab, What are the excretory organs of crab

What are the excretory organs of crab

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd