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Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.
One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.
Define Methods of Estimation of Ascorbic Acid (Vitamin C)? Ascorbic acid is also known as vitamin C which is a water soluble vitamin. As you already know, vitamins are a group
It is immune difficult mediated hypersensitivity. Typical manifestations contain rheumatoid arthritis, serum sickness, necrotizing etc.
Interact with the Parent and Child to Exchange Information At this time the nurse can learn why the child has come to the hospital and the concerns and expectations he and hi
Indications for Gingival Curettage - Endodontic Surgery a) Persistent periradicular pathosis following endodontic treatment, b) Periradicular lesion that enlarges after endo
T ypes of hormones - (i) Releasing and Inhibitory hormones - Hormones of hypothalamus and kidney (renin) which control secretion of trophic hormones. (ii) Trophic hormon
Q. Explain about Podophyllum? These species were long used by indigenous people in Americea and Asia, including for the treatment of skin cancers and warts. The combination of
INSULIN Linear polymer of b-D-Glucose. Insulin is small-sized (25 - 35 residues) fructosan homopolysaccharide which is a reserve carbohydrate present in roots, tubers and
explain glycolysis briefly? elaborate
PSYCHOSOCIAL THERAPY: Psychosocial therapy is one of the important treatment modaIities used for the patients with mental disorders. It is given along with other therapies or
Define Water as a temperature regulator? Water plays an important role in the distribution of heat throughout the body and the regulation of body temperature. Heat is generate
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