Clone (verb), Biology

Assignment Help:

Clone (verb) is the action of duplicating the genetic material within a vector. To clone the piece of DNA, one would insert it into some type of the vector (like, a plasmid) and put the resultant construct into a host (generally a bacterium) so that the plasmid and insert replicate with the host. An individual bacterium is made isolated and grown and the plasmid containing the "cloned" DNA is re-isolated from bacteria, at which point there will be many millions of copies of the DNA - essentially an limitless supply. Actually, an investigator wishing to clone some gene or cDNA rarely has that DNA in a purified form, so practically speaking, to "clone" something involves screening a cDNA or genomic library for the desired clone. See also "Probe" for a description of how one might start a cloning project, and "Screening" for how the probe in used.

One can also clone more complex organisms, with considerable difficulty. The much-publicized Scottish research that resulted in the sheep 'Dolly' exemplifies this approach.


Related Discussions:- Clone (verb)

Compare and contrast the effect of a deletion, Compare and contrast the eff...

Compare and contrast the effect of a deletion in the operator of the lactose operon with one in the operator of the histidine operon.

Transport of instruments to the sterilization area, Most dental offices hav...

Most dental offices have a designated area for instrument reprocessing that is separate from the dental treatment room. This is ideal, since cleaning, sterilizing and storing instr

Carbohydrate case study, CT is a 3 year old female who develops signs of hy...

CT is a 3 year old female who develops signs of hypoglycemia in the early morning unless she is fed during the night.  CT eats balanced meals for her age group during the day, with

What will be the final volume, Suppose a sample of 200 microliters of blood...

Suppose a sample of 200 microliters of blood needs to be diluted 1/50. How much diluents should be added and what will be the final volume? Show all steps.

Mycotic diseases-sheep, Goat and sheep M. mycoides subsp. capri is the...

Goat and sheep M. mycoides subsp. capri is the cause of caprine pleuropneumonia, a serious malady of goats. The disease is prevalent in certain parts of this country. The mort

Characters of vitamins, Characters of vitamins: Vitamins are organic...

Characters of vitamins: Vitamins are organic substances. They are micronutrients and are required in small quantities. They are not synthesised in the body and therefo

Effect in controlling communicable disease, Early campaigns of the 19th cen...

Early campaigns of the 19th century that focused on sanitation, hygiene, housing, and nutrition had little effect in controlling communicable disease due to flawed rationale based

Obelia, Economic importance of obelia

Economic importance of obelia

Can you explain aneurysm of aorta, Q. Can you explain Aneurysm of Aorta? ...

Q. Can you explain Aneurysm of Aorta? CXR findings are an enlargement of the involved portion of the aorta. A focal dilatation may simulate a mass or adenopathy. A more general

Explain about the rna viruses - carcinogenic, Explain about the RNA Viruses...

Explain about the RNA Viruses - carcinogenic? All oncogenic RNA viruses are retroviruses. They are of 2 types. They are acute transforming retroviruses and slow transforming re

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd