Chromosome walking, Biology

Assignment Help:

Chromosome walking is the technique for cloning everything in genome around the known piece of DNA (the begning probe). You screen the genomic library for all the clones hybridizing with probe, and then figure out which one extends furthest into surrounding DNA. The most distal segment of this most distal clone is then taken in use as a probe, so that ever more distal areas can be cloned. This has been taken in use to move as much as 200 kb away from the given begning point (an immense undertaking). In general it is used to "walk" from a starting point towards some nearby gene in order to clone that specific gene. It is also used to obtain the remainder of a gene when you have isolated a part of it.


Related Discussions:- Chromosome walking

Female reproductive disorders-hydro allantois, Hydrops allantois (Hydro all...

Hydrops allantois (Hydro allantois) This condition is uncommonly seen in dairy cattle and buffaloes. Accumulation of excessive allantoic fluid may be progressive after mid ges

Which is the type of nitrogen waste, Which is the type of nitrogen waste el...

Which is the type of nitrogen waste eliminated by beings of the class Reptilia? These beings excrete mainly uric acid. This substance is less toxic than ammonia and it can be k

Determine the chemical stage of photosynthesis, Which are the subproducts o...

Which are the subproducts of the photochemical stage that are essential for the chemical stage of photosynthesis? The chemical stage of photosynthesis depends on NADPH and ATP

Define hormonal responses to injury, Define Hormonal Responses to Injury? ...

Define Hormonal Responses to Injury?  A number of hormonal changes take place in patients following injury. There is a marked rise in the counter regulatory hormones, viz., glu

Define food intake during polar expeditions, Define Food Intake during Pola...

Define Food Intake during Polar Expeditions? Observations made by Easty (1967) at Halley Bay, the British Antarctic survey base during 1961-62 expeditions indicate mean calorie

Urine sugar testing, Testing for urine sugars is not recommended for either...

Testing for urine sugars is not recommended for either diagnosis or monitoring of patients with diabetes. This is because a urine sugar is not a reliable test. When no facilities a

Fire and cookery , Fire and Cookery : Fire and Cookery where and when f...

Fire and Cookery : Fire and Cookery where and when fiie came to be used  is not known.  Fire, to start with, must have been a frightening thing, giving rise to many myths and l

Biological responses controlled by phytochrome, Biological Responses Contro...

Biological Responses Controlled by Phytochrome Phytochrome responses are those which are controlled reversibly by red and far-red light. These can be broadly categorised as:

Define water as a source of dietary minerals, Define Water as a source of d...

Define Water as a source of dietary minerals? Although water is composed of only oxygen and hydrogen, the water we drink or use in food preparation can contain significant amou

Human impact on environment, questions and answers on human impacts on envi...

questions and answers on human impacts on environment

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd