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Chromosome jumping is the technique whereby one begins with a piece of DNA from one area of a chromosome, and obtains clones from nearby areas without cloning everything in between (as in chromosome walking). One round of jumping yields the new clones at the distances of several tens of kb away from the beginning point. In practice, this method is used when classical genetics proves that a known piece of DNA is located on the chromosome close to the gene you would like to clone (like a human disease gene). By cloning the fragments some distance away in both the directions from the known fragment, one might attain (1) fragments further from the required gene (which are discarded); (2) the fragments are even more closely linked to the required gene (in which case one goes for another round of jumping); or (3) fragments from within required gene - the optimal result.
Inorganic Nitrogen and Sulphur Metabolism Productivity in agriculture, forestry and other ecosystems is basically limited by the availability of nitrogen nutrients like nitrat
basic difference beyween gymnosperm,angiosperm and pteridophytes.
Members of phylum animal like protist that are only free living
Needlestick/sharp instrument injuries are of major concern to health care workers. All of the significant exposures reported in the academic year 2003/04 in our Faculty, were injur
What is Invertebrates? Invertebrates: About 99% of all the animals lack backbones, and are invertebrates! Invertebrates include the phylum Arthropoda, or the animals with joi
What are the classes into which the phylum Arthropoda is divided? What are the three main ones and some of their representative species? The three major classes of arthropods a
Define Criteria for Assessment of Vitamin D Status? You may recall the events involved in calcium homeostasis described earlier in this section. We studied that sufficient 25-O
Determine the uses of Potassium Water soluble and exchangeable potassium is readily available to plants. This is extracted with Morgan reagent (10% sodium acetate in 3% acetic
We now understand that mutations that cause the inhibition of apoptosis are found in tumours. Because proliferation itself is not induced by the inhibition of apoptosis, explain ho
pls explan 2 puc lesson
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