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Chromosome jumping is the technique whereby one begins with a piece of DNA from one area of a chromosome, and obtains clones from nearby areas without cloning everything in between (as in chromosome walking). One round of jumping yields the new clones at the distances of several tens of kb away from the beginning point. In practice, this method is used when classical genetics proves that a known piece of DNA is located on the chromosome close to the gene you would like to clone (like a human disease gene). By cloning the fragments some distance away in both the directions from the known fragment, one might attain (1) fragments further from the required gene (which are discarded); (2) the fragments are even more closely linked to the required gene (in which case one goes for another round of jumping); or (3) fragments from within required gene - the optimal result.
Noncompetitive inhibition In this type of inhibition, the inhibitor binds at a site on the enzyme other than catalytic site. As there is no competition between the s
Q. Explain about Iceberg Phenomenon? Phenomenon means a fact or occurrence that appears. Let us see how iceberg phenomenon is applied to Diabetes Mellitus (DM). Cases of dia
Precautions for Preparation of Selective and Differential Medium 1. Dissolve ingredients one by one. 2. Check and set the medium to appropriate pH. 3. Autoclave the mediu
Explain Components of second heart sounds? The first component of S 2 is due to closure of aortic valve (A,). The second component is due to closure of pulmonary valve (P 2 ).
What are the 3 phases of seed formation
ENDOPLASMIC RETICULUM (ER) Eucaryotic celIs have two major compartments- nucleus and cytoplasm. Cytoplasm was known to have no structure until the discovery of electron micrsscop
what are inorganic species
What is the chemical nature of carbohydrates? The name carbohydrate owes its origin to the fact that most substances of this class have empirical formulae suggesting that they
Advice on Discharge : Patients are allowed to go home on 8th or 9th day. It could be even earlier after off pump coronary artery surgery (OPCAB). They should gradually increase t
Feline calcivirus infection This disease is caused by a virus under the family Caliciviridae. Its symptoms include rhinitis, tracheitis and pneumonia. The animals are lethargi
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