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Chromosome jumping is the technique whereby one begins with a piece of DNA from one area of a chromosome, and obtains clones from nearby areas without cloning everything in between (as in chromosome walking). One round of jumping yields the new clones at the distances of several tens of kb away from the beginning point. In practice, this method is used when classical genetics proves that a known piece of DNA is located on the chromosome close to the gene you would like to clone (like a human disease gene). By cloning the fragments some distance away in both the directions from the known fragment, one might attain (1) fragments further from the required gene (which are discarded); (2) the fragments are even more closely linked to the required gene (in which case one goes for another round of jumping); or (3) fragments from within required gene - the optimal result.
Define Reagents for Estimation of Calcium Using EDTA Titrimetric Method? 1. Buffer solution: Dissolve 16.9 g NH 4 CI in 143 ml NH 4 OH. To this add 50 ml of solution containing
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ORIGIN OF E.R. (1 ) Originates from plasma membrane. (2 ) According to Haguenau, E.R.originates from Nebenkern (Germ center). Nebenkern is a group of conce
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Q. What are the main interspecific ecological interactions? The major harmonious interspecific ecological interactions are: protocooperation, commensalism and mutualism. The ma
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