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Chromosome jumping is the technique whereby one begins with a piece of DNA from one area of a chromosome, and obtains clones from nearby areas without cloning everything in between (as in chromosome walking). One round of jumping yields the new clones at the distances of several tens of kb away from the beginning point. In practice, this method is used when classical genetics proves that a known piece of DNA is located on the chromosome close to the gene you would like to clone (like a human disease gene). By cloning the fragments some distance away in both the directions from the known fragment, one might attain (1) fragments further from the required gene (which are discarded); (2) the fragments are even more closely linked to the required gene (in which case one goes for another round of jumping); or (3) fragments from within required gene - the optimal result.
Explain the Inoculating Loops and Needles? These are most commonly used tools for inoculation. The inoculating loop consists of insulating handle at the end of which inoculatin
In terms of western medicines, over 3000 antibiotics - including penicillin and tetracycline - are obtained from micro-organisms. Another product of micro-organisms i.e. cyclospori
Intracellular sodium concentration of the motor neuron A complete motor neuron is removed from a frog and placed in normal physiological saline at 1 AM. The neuron is healthy.
Tumor Suppressor Genes Tumor suppressor genes or anti-oncogenes generally inhibit cell division in cooperation with proto-oncogenes. The very existence of these genes prevent
Obtain the sequence for the gene and the sequence for the RNA transcript in FASTA format. If there is more than one transcript choose and appropriate one and explain your choice. U
Q. What structure of the eye produces tears? Lachrymal gland, which is a part of larger lachrymal apparatus, produces tears that flow over anterior surface of the eye. Most of
Define the Growth and Development of the infant? Infancy is a period of rapid growth. During the first year of life, the infant grows and develops far more rapidly than at any
Features of the Gastrulation The significant features of the gastrulation are: a) Proteins of many new types that were not present in the egg or blastula begin to be synthe
Q. What is the group of the phylum Chordata that first colonized the terrestrial environment? From which habitat did they come? Partially aquatic partially terrestrial animals,
Q. Conditions Necessary for Outbreak of staphylococcal food? The following conditions are necessary for an outbreak of staphylococcal food poisoning: 1. The food must conta
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