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Chromosome jumping is the technique whereby one begins with a piece of DNA from one area of a chromosome, and obtains clones from nearby areas without cloning everything in between (as in chromosome walking). One round of jumping yields the new clones at the distances of several tens of kb away from the beginning point. In practice, this method is used when classical genetics proves that a known piece of DNA is located on the chromosome close to the gene you would like to clone (like a human disease gene). By cloning the fragments some distance away in both the directions from the known fragment, one might attain (1) fragments further from the required gene (which are discarded); (2) the fragments are even more closely linked to the required gene (in which case one goes for another round of jumping); or (3) fragments from within required gene - the optimal result.
he Lac Repressor protein, which we will talk about a bit later in the course, is important for controlling expression of a number of genes in E. coli. There are about 10 copies of
Please explain the differences between an integral membrane protein, a Davison-Danielli peripheral membrane protein, and a protein in which alpha-helices would not be found.
Composition - Culture Medium A standard plant tissue culture medium (Basal medium) contains all the essential macro elements (Carbon, hydrogen, oxygen nitrogen, phosphorus, su
Define Renal Function & cardiovascular - change related with ageing? Changes associated with the cardiovascular and renal function: The progressive accumulation of athermanous
1. In cattle, the polled condition (H) is dominant to the horned condition (h). A cross between an individual with red coat (R) and white coat (W) results in roan (RW). A polled, r
whats polymorphic
44+(XXY) is in which syndrome?
How do you determine net atp production after complete oxidation to CO2 and H2O using mitochondrial ?-oxidation, the TCA cycle, the mitochondrial electron transport chain and oxida
ENDOPLASMIC RETICULUM It is also known as Endoskeleton of the cell, Endomembranous system of the cell. Garnier (1897) first observed them and called Ergastoplasm. E.R.
What do you understand by Mesocoel? The middle of three coelomic spaces found in tripartate body plan characteristic of deuterostome lineage of animals. Other coelomic compartm
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