Chain termination method, Biology

Assignment Help:

An incubation  combination  is set up having  the single-stranded  DNA template, DNA polymerase  I, the primer and all 4 deoxyribonucleoside triphosphates (dTTP, dGTP, dCTP, dATP), one of that is radioactively  labeled plus a one 2'3' dideoxyribonucleoside triphosphate  analog, say ddGTP. Within this incubation the DNA polymerase   starts copying template molecules   by extending   the bound primer.  As the new DNA  strand  is synthesized,  every  time which dGTP should  be  incorporated   there  is  a  chance  which  ddGTP  will  be  incorporated instead. If this happens, no further chain elongation  can occur because dideoxy analogs  lack  the  3'-OH  group  needed  to  make  the  next  3'5'  phosphodiester bond.  Thus this particular chain stops at this point.  In this first incubation combination, a huge population of templates is being copied and each new strand will stop randomly at positions where a G must be added to the latest synthesized strand.  Thus, for every G in the complementary sequence there will be some new DNA strands which have terminated at that point shown in the figure.

 

541_22.png

Figure: DNA sequencing by the chain termination (Sanger) method.


Related Discussions:- Chain termination method

Parasitology, how trematodes/nematodes adapt to their parasitic mode of fee...

how trematodes/nematodes adapt to their parasitic mode of feeding

Coronary angiography, Q. Can you explain about Diagnostic coronary angiogra...

Q. Can you explain about Diagnostic coronary angiography? Diagnostic coronary angiography has become one of the primary components of cardiac catheterization. In a coronary ang

Differences between mitosis and meiosis, DIFFERENCES BETWEEN MITOSIS AND...

DIFFERENCES BETWEEN MITOSIS AND MEIOSIS    EVENTS  MITOSIS  MEIOSIS  Occurrence  In all the body cells including

Dietary management for ulcerative colitis, Q. Dietary Management for ulcera...

Q. Dietary Management for ulcerative colitis? Proper dietary management is important for maintaining a good nutritional status of the patient. Long-term management is generally

Acid hydrolysis of non-reducing sugar, Q. Acid Hydrolysis of Non-Reducing S...

Q. Acid Hydrolysis of Non-Reducing Sugar? It involves the acid hydrolysis of non-reducing sugars to reducing sugars. It is also known as the process of inversion. Acid hydrolys

Describe about psycho-social change in children, Describe about Psycho-soci...

Describe about Psycho-social Change in children? Adolescence is a period of maturation for both mind and body. Along with the physic4 growth, emotional and intellectual develop

Zoology, Give the salient features of phylum protozoa

Give the salient features of phylum protozoa

Explain about the biosensors and caramelization, Explain about the Biosenso...

Explain about the Biosensors and Caramelization? Biosensors:  A device that utilizes biological materials to monitor the presence of several chemicals in a substance. Carame

Explain about the biofertilizers, Explain about the Biofertilizers Biof...

Explain about the Biofertilizers Biofertilizers, are the cultures of microorganisms used for inoculating seed or soil or both under ideal conditions to increase the availabilit

Define intermediate level of the numeric pyramid, In the short term what wi...

In the short term what will happen to the levels above and below a population of secondary consumers of a numeric pyramid if a large number of individuals from this population dies

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd