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An incubation combination is set up having the single-stranded DNA template, DNA polymerase I, the primer and all 4 deoxyribonucleoside triphosphates (dTTP, dGTP, dCTP, dATP), one of that is radioactively labeled plus a one 2'3' dideoxyribonucleoside triphosphate analog, say ddGTP. Within this incubation the DNA polymerase starts copying template molecules by extending the bound primer. As the new DNA strand is synthesized, every time which dGTP should be incorporated there is a chance which ddGTP will be incorporated instead. If this happens, no further chain elongation can occur because dideoxy analogs lack the 3'-OH group needed to make the next 3'5' phosphodiester bond. Thus this particular chain stops at this point. In this first incubation combination, a huge population of templates is being copied and each new strand will stop randomly at positions where a G must be added to the latest synthesized strand. Thus, for every G in the complementary sequence there will be some new DNA strands which have terminated at that point shown in the figure.
Figure: DNA sequencing by the chain termination (Sanger) method.
List the parameters under which you will evaluate implant prosthesis The various parameters under which implant prosthesis can be evaluated are: i) Assessment of mobility.
wht is the main quality of this phylum
Q. What are the few examples of the control and informative function of organic molecules? Based on genetic information, organic molecules control the entire work of the cell.
What are complementary genes? Does this inheritance pattern obey Mendel's second law? Complementary genes are the different genes that act together to determine a given phenoty
explain protozoa taxonomy
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Culture Conditions The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of l
details and terms used in growth and development?
Select all that are true or correct regarding a buffer: alternately donating and accepting ions a set of chemicals that work together to resist pH change often a weak acid or base
Zygotic or Suspensor Polyembryony Cleavage of the apical cells of the globular or filamentous proembryo produced by the zygote may result in two or more embryos in a seed. Th
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