Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
An incubation combination is set up having the single-stranded DNA template, DNA polymerase I, the primer and all 4 deoxyribonucleoside triphosphates (dTTP, dGTP, dCTP, dATP), one of that is radioactively labeled plus a one 2'3' dideoxyribonucleoside triphosphate analog, say ddGTP. Within this incubation the DNA polymerase starts copying template molecules by extending the bound primer. As the new DNA strand is synthesized, every time which dGTP should be incorporated there is a chance which ddGTP will be incorporated instead. If this happens, no further chain elongation can occur because dideoxy analogs lack the 3'-OH group needed to make the next 3'5' phosphodiester bond. Thus this particular chain stops at this point. In this first incubation combination, a huge population of templates is being copied and each new strand will stop randomly at positions where a G must be added to the latest synthesized strand. Thus, for every G in the complementary sequence there will be some new DNA strands which have terminated at that point shown in the figure.
Figure: DNA sequencing by the chain termination (Sanger) method.
Explain the term - Muscle Contraction Headache This is one of the most common headaches. It is also known as tension or nervous headaches. They result from sustained contractio
The gene is a distinct portion of a cell's DNA. Genes are coded instructions for making all the body needs, mainly proteins. Human beings have about 25,000 genes. Researchers have
Production Efficiency - Ecosystem The maximum amount of solar energy harvested by plants is about 5 per cent but the average for green plants, on the whole is only a small fra
Gluconeogenesis: When diet is deficient incarbohydrates. Glucose or glycogen is formed from noncarbohydrate compounds principally from certain amino acids and the glycerol of
GLANDS - 1 . LACRYMAL GLANDS - Tear glands numbers are 3. Its secretion is aquous & salty, keeps eye ball wet, give nourishment to cornea. Lysozyme also present
RESPIR A T O R Y PIGMENTS - Resp. pig. is generally Hb (Fe is attached to globine by chellate bond). In Earthworm Erythroqruorine present. Haemocyanine (Cu) genera
Can stroke be prevented Brain experts are convinced that several strokes can be prevented with proper attention to lifestyle factors which increase risk, including smoking, exc
the excretory waste products formed breakdown of protein
Determine fat-soluble vitamins Recommended for Infant? ICMR (1990) does not give recommendations for vitamins D, E and K. As you already know, vitamin D is expected to be met
Explain brifly Ribosomes, Endoplasmic Reticulum, Golgi Apparatus ? Ribosomes, Endoplasmic Reticulum, Golgi Apparatus : The cytoplasm contains many ribosomes, which are sphe
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd