Chain termination method, Biology

Assignment Help:

An incubation  combination  is set up having  the single-stranded  DNA template, DNA polymerase  I, the primer and all 4 deoxyribonucleoside triphosphates (dTTP, dGTP, dCTP, dATP), one of that is radioactively  labeled plus a one 2'3' dideoxyribonucleoside triphosphate  analog, say ddGTP. Within this incubation the DNA polymerase   starts copying template molecules   by extending   the bound primer.  As the new DNA  strand  is synthesized,  every  time which dGTP should  be  incorporated   there  is  a  chance  which  ddGTP  will  be  incorporated instead. If this happens, no further chain elongation  can occur because dideoxy analogs  lack  the  3'-OH  group  needed  to  make  the  next  3'5'  phosphodiester bond.  Thus this particular chain stops at this point.  In this first incubation combination, a huge population of templates is being copied and each new strand will stop randomly at positions where a G must be added to the latest synthesized strand.  Thus, for every G in the complementary sequence there will be some new DNA strands which have terminated at that point shown in the figure.

 

541_22.png

Figure: DNA sequencing by the chain termination (Sanger) method.


Related Discussions:- Chain termination method

Genetics, Ask question importance of genetics in human life #Minimum 100 w...

Ask question importance of genetics in human life #Minimum 100 words accepted#

Explain the cori cycle, The  Cori cycle a)  Pyruvate formed from glucos...

The  Cori cycle a)  Pyruvate formed from glucose is converted  to lactate by lactate dehydrogenase in the muscle cell. b)  Lactate is released into  the blood and taken up b

Bacteria is an obligate anaerobe, Q. What is signifying when it is said tha...

Q. What is signifying when it is said that a bacteria is an obligate anaerobe? Obligate anaerobes are those living beings that do not survive in the presence of oxygen. For exa

Explain the miracidium larva, Explain the Miracidium larva? Miracidium ...

Explain the Miracidium larva? Miracidium is the first stage larva that comes out from the fertilized egg. i) Miracidium is an oval, microscopic, flattened larva which appear

Atelectasis and pneumonia - complications of cardiac surgery, Atelectasis a...

Atelectasis and Pneumonia Signs and symptoms are LV failure, hypovolemia, hypervolemia or renal vasoconstriction. Decreased breath sounds, poor ABG and poor cough reflex

Direct use values of biodiversity, Q. Direct use values of biodiversity? ...

Q. Direct use values of biodiversity? Direct use values are for those goods that are ensured directly e.g. food and timber. Maintaining a wide range of components of biological

Dietary management of dyspepsia, Q. Dietary management of dyspepsia? Ke...

Q. Dietary management of dyspepsia? Keeping in mind the etiology, symptoms and complications of dyspepsia it must be clear that treatment and management of [his disorder does n

Bone loss - criteria for endosteal implants, Q. Bone Loss - criteria for en...

Q. Bone Loss - criteria for endosteal implants? Crestal bone loss after intial healing is a primary indicator of the need for initial preventive therapy. Early loss of crestal

Explain adverse effects of interferon alfa, Adverse Effects Of interferon a...

Adverse Effects Of interferon alfa Intramuscular or subcutaneous injection of interferon is commonly associated with an influenza-like syndrome, especially during the first wee

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd