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CAT assay is an enzyme assay. CAT stands for the chloramphenicol acetyl transferase, a bacterial enzyme which inactivates the chloramphenicol by acetylating it. CAT assays are many times performed to test the function of a promoter. The gene coding for CAT is linked onto the promoter (transcription control area) from another gene, and the construct is "transfected" into cultured cells. The quantity of CAT enzyme produced is taken to indicate transcriptional activity of the promoter (relative to other promoters which should be tested in parallel). It is simple to perform a CAT assay than it is to do a Northern blot, so CAT assays were common techniques for testing the effects of sequence changes on the promoter function. It is largely supplanted by the reporter gene luciferase.
Prevention of Infection 1. Strict asepsis practiced in ICU. 2. All the invasive lines are covered with sterile-drape. 3. Antibiotics-broad-spectrum ones are adm
Question 1: Describe upon CMC information about drug substance to be submitted to IND for Phase 3 studies. Definition of CMC Explain about CMC information about drug subs
Demonstration of Diversity of Plant and Animal - Taxonomy A third objective is the demonstration of tremendous diversity of the plant and animal world and its relation to man's
Explain Energy demand for Sedentary or Light Activity Lifestyles? These people have occupations that do not demand much physical effort, are not required to walk long distances
migration
Heteromorphic Types - Intra specific Incompatibility Plants of the same species produce flowers that differ in morphology. This involves two (distyly) or three (tristyly) morp
What is the best microscope to get a detailed view of the parts inside of a preserved plant cell? Transmission electron microscope Scanning electron microscope Compound li
Nursing Interventions of a Patient with Acute Alcohol: Intoxication and Withdrawal Some nurses find it very difficult to work with the alcoholics. This is particularly true w
Q. How Bone density affect osseointegration? The most important bone property is density which is influenced by factors such as patient age and genetics. Higher density bones h
#life history
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